Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0001301

Physical Location & Seq

Genomic Coordinates Reverse (-)

59412606 .. 59419723

Gene Structure 7118 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00266040.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2550 bp

ATGGCGTTTGAAGCTTGTTGCTTACGGCTGCTCGTGGTACTAATTGTGTTTCTAAATGTAGAATCCAAGTTCATGGCTTACAATACAGCGTCGCAGATTGTCCCCGGAAAGCTTAACGTTCATTTAGTTCCTCACACCCATGACGATGTCGGATGGCGGAAAACAGTTGACCAGTACTACACCGGTGCCAATAATTCTCTCCTGGCATTTTTTCAACTATGGTGGAGAGAACAGAGCGAGGCGATCCGAGACACAGTCAAGCAGCTAGTCAGCTCTGGCCAACTGGAATTCATAAATGGGGGGATGTGCATGCACGATGAGGCTGCTACGCATTACATTGATATGATTGATCAGACGACTCTAGGGCATCGATTTATTAAGCAAGAGTTCAATGTGACTCCAAGAGTTGGCTGGCAAATCGATTCGTTTGGACATTCTTCTGTGCAGGCTTACCTTTTGGCGGCTGAGATTTTTGCTGGTGCATTCCCTAGGCTATATGTTCCTCCTTCTGATAATTTCTACTTTGACACTGATGACGTCCTTGCCCCTTTTGTACAAGATGATATGAATCTTGGTGACTACAATGTGGCAGATCGCGTTAATGATTTTGTATCTGAAGCAATATCACAAGCAAACATTACTCGTACAAATCATATCATGTGGACCATGGGAAAGGATTTCGCATATCAATATGCTAATTCATGGTTCAGTCAGATGGACAAATTCATTCATCATGTGAATCAAGTTAGTTCATCAGTGCATGCTGATGGGCGTGTCAATGCCCTATACTCAACCCCGTCAATATACACCGATGCCAAATATGCAACAAATGAGTCCTGGCCAATCAAGAGCAGCAAGGCAATTGGAATTTTTAAGGGAATAAGTAAATCAGGGCCTAACACAGATTCTCTGGCTGATGCTTTAGCAATTGCTCAACATCATGATGCAGTTAGTGGCACATCTAGGCAGCATGTAGCTGATGATTATACAAAACGACTTTCAATAGGTTATAAGGAGGCTGAGAAGGTTGTTGCAGAATCACTTTCTTGCTTGATAAGTTCAAGATCAAGCACTGGTTGCAAGAGTGTTATTGTTGTCTATAACCCTCTGGGATGGAAAAGAGAGGATGTGATAAAGATTCCTGTTGTCAGTGAAAAAGTCACTGTAAAGGACTCCACCGGAAAGGAAATTGAATCACAGATTCTACCGCTGCTTAATGCATCTGTCAGCATAAGAAATGACCATGTTAAGGCATATTTGGGTATAGCACCGAGTGTGACCACAAGTTATTGGCTTGCATTTTCAGCAACTGTACCACCTCTTGGTTTTAGCACTTACATGGTCTCAAGTGCTGCCTCACAGACAGCTACTACTTCAGAGAGACATACAGTGTACAAGACAGAAACAAGTCAAAATGAAACAATAAAAGTTGGGCCTGGAAACTTGAAACTCATTTATTCTGGAAACGATGGAAAACTGATTCAGTACACAAACAGCAGAAGCTCGGTCAAAGAGTTGGTAAGGCAATCATTTAGCTACTACCCTGGAGATGATGGAAGTGTGGTGGATTACGGTGAGGATGGAAATCCGCATTTTCAGGTCACTAGAGTGTACAGGGAAAAAGAGCATGCTGAGATTGAATTTACTATAGGGCCTATACCAGTTGATGATGGAATAGGCAAAGAGATTGTAACTAAGATTACAACCAGCATGAGAACCAACAAACAATTCTACACAGATTCTAACGGGCGCGATTTTATTCAAAGAATTCGAGACCGCAGAAAAGACTGGGACCTGGAAGTAAACCAACCTGTTGCAGGAAACTATTATCCGATTAATCTGGGAATCTATACGAAAGATAACAACACAGAGCTGTCAGTATTAGTGGATAGATCTGTAGGGGGATCCAGCATTGTGGACGGGCAATTGGAACTGATGCTTCATAGGAGGTTGCTTGCAGATGATGACAAAGGTATTGCAGAACAACTAAATGAAACGGTGTGCATTGAACATGATTGCAAAGGGCTAACAATTACAGGAAAGTACCACCTGAGAATTGATCCTTTAGGAGAGGGTGCTAAGTGGCGTCGATCATTTGGTCAGGAGATATATTCTCCATTTCTATTAGCCTTCACAGAACAAAATGGAGATGACTGGACAAACTCTCATGTCACAACCTTTTCGGGAATGGATCCTTCCTACACTTTACCTGATAATGTTGCAATTATAACTCTCCAGGAGCTGGAAGATGGGAAAGTTCTCTTTCGCCTGGCGCATTTATACGAGGTTGAAGAGGACAAGCATCTTTCAGTTATGGCAAGTGTGGAACTGAAAAAGGTGTTTGCAAAGAAGAAGATAAAGCATATAACAGAAATGAGCTTATCTGCTAACCAAGAAAGAAGGGAAATGGAAAGGAAGAGACTGGCTTGGAATGTGCAAGGCTCTTCTGAGGAAGGACCGAAAGGCTTGAGGGGAGGACCTGTTGATCCAACAAAGCTAGTGGTGAATCTTGCTCCAATGGAAATTCGAACATTTACCATCGACTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Alpha-mann_mid Glyco_hydro_38 Glyco_hydro_38C

Protein Analysis

849

Amino Acids

95.21

Weight (kDa)

5.76

Isoelectric Point (pI)

35.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	69 - 283	8.3e-40	Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid	PF09261	290 - 351	7.8e-11	Alpha mannosidase middle domain
Laman-like_dom	PF21260	425 - 448	5.3e-09	Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C	PF07748	533 - 655	4.7e-28	Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2	PF17677	739 - 845	2.5e-08	Glycosyl hydrolases family 38 C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001301)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13980 AT5G13980 AT5G13980
fragaria_vesca	FvH4_5g09420 FvH4_5g09421 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272
malus_domestica	MD06G1131600.v1.1 MD09G1002900.v1.1 MD17G1007000.v1.1
prunus_persica	Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.5G142600_v2.0.a1 Prupe.5G142600_v2.0.a1
pyrus_communis	pycom06g12300 pycom06g12310 pycom111g00240 pycom17g00260
rosa_chinensis	RchiOBHm_Chr2g0176471 RchiOBHm_Chr2g0176481 RchiOBHm_Chr7g0188771 RchiOBHm_Chr7g0188781
rosa_laevigata	RLG00000004661 RLG00000004662 RLG00000022416
rosa_multiflora	Rmu_sc0000303.1_g000057 Rmu_sc0003642.1_g000008 Rmu_sc0011904.1_g000012
rosa_roxburghii	Rroxscaffold_2G00076370 Rroxscaffold_3G00266040 Rroxscaffold_3G00266050
rosa_rugosa	Rorug02G0595000 Rorug06G0493700
rosa_samantha	Rh2AG673300 Rh2BG684600 Rh2CG647400 Rh2DG698200 Rh7AG098300 Rh7AG098400 Rh7BG099900 Rh7BG100200 Rh7CG101600 Rh7CG101700 Rh7DG100100 Rh7DG100200
rosa_wichuraiana	Rw0G010390 Rw2G055220 Rw7G008480 Rw7G008490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1011, 2228
AatII	GACGTC	1 cut(s)	540
AccB1I	GGYRCC	1 cut(s)	185
AccB7I	CCANNNNNTGG	3 cut(s)	407, 1322, 2242
AccII	CGCG	2 cut(s)	597, 1752
AciI	CCGC	5 cut(s)	157, 461, 1208, 1589, 1777
AclI	AACGTT	1 cut(s)	117
AclWI	GGATC	7 cut(s)	238, 1899, 1912, 2054, 2186, 2199, 2480
AcoI	YGGCCR	2 cut(s)	277, 839
AcsI	RAATTY	6 cut(s)	287, 722, 867, 1640, 1767, 2523
AcuI	CTGAAG	2 cut(s)	636, 1359
AcyI	GRCGYC	2 cut(s)	537, 2086
AdeI	CACNNNGTG	1 cut(s)	1274
AfaI	GTAC	9 cut(s)	39, 176, 555, 646, 1314, 1394, 1487, 1613, 2045
AfiI	CCNNNNNNNGG	6 cut(s)	407, 460, 1249, 1322, 1817, 2242
AgeI	ACCGGT	1 cut(s)	182
AjnI	CCWGG	7 cut(s)	201, 836, 1435, 1543, 1794, 2235, 2268
AjuI	GAANNNNNNNTTGG	2 cut(s)	1712, 1744
AleI	CACNNNNGTG	1 cut(s)	1607
Alw26I	GTCTC	5 cut(s)	243, 1348, 1375, 1767, 2413
AlwI	GGATC	7 cut(s)	238, 1899, 1912, 2054, 2186, 2199, 2480
AlwNI	CAGNNNCTG	2 cut(s)	1310, 2242
AoxI	GGCC	5 cut(s)	277, 839, 893, 1433, 1652
ApeKI	GCWGC	7 cut(s)	28, 262, 323, 852, 967, 1210, 1352
ApoI	RAATTY	6 cut(s)	287, 722, 867, 1640, 1767, 2523
AseI	ATTAAT	1 cut(s)	1836
AsiGI	ACCGGT	1 cut(s)	182
AspA2I	CCTAGG	1 cut(s)	488
AspLEI	GCGC	2 cut(s)	1752, 2275
AspS9I	GGNCC	7 cut(s)	663, 893, 1433, 1652, 1792, 2456, 2477
AsuC2I	CCSGG	1 cut(s)	105
AsuHPI	GGTGA	3 cut(s)	587, 1586, 2515
AsuII	TTCGAA	1 cut(s)	2527
AvaII	GGWCC	4 cut(s)	663, 1792, 2456, 2477
AvrII	CCTAGG	1 cut(s)	488
BalI	TGGCCA	2 cut(s)	279, 841
BamHI	GGATCC	2 cut(s)	1904, 2191
BanI	GGYRCC	1 cut(s)	185
BauI	CACGAG	1 cut(s)	32
BbvI	GCAGC	7 cut(s)	15, 274, 310, 864, 979, 1197, 1339
BceAI	ACGGC	1 cut(s)	41
BcgI	CGANNNNNNTGC	2 cut(s)	305, 339
BciT130I	CCWGG	7 cut(s)	203, 838, 1437, 1545, 1796, 2237, 2270
BclI	TGATCA	1 cut(s)	349
BcnI	CCSGG	1 cut(s)	105
BcoDI	GTCTC	5 cut(s)	243, 1348, 1375, 1767, 2413
BfaI	CTAG	7 cut(s)	266, 362, 489, 963, 1605, 2498, 2548
BfmI	CTRYAG	2 cut(s)	1647, 1896
BglII	AGATCT	1 cut(s)	1892
BisI	GCNGC	8 cut(s)	29, 263, 324, 462, 853, 968, 1211, 1353
BlnI	CCTAGG	1 cut(s)	488
BlsI	GCNGC	8 cut(s)	30, 264, 325, 463, 854, 969, 1212, 1354
BmcAI	AGTACT	1 cut(s)	176
Bme1390I	CCNGG	8 cut(s)	105, 203, 838, 1437, 1545, 1796, 2237, 2270
Bme18I	GGWCC	4 cut(s)	663, 1792, 2456, 2477
BmgT120I	GGNCC	7 cut(s)	663, 893, 1433, 1652, 1792, 2456, 2477
BmiI	GGNNCC	4 cut(s)	187, 1793, 1906, 2193
BmrFI	CCNGG	8 cut(s)	105, 203, 838, 1437, 1545, 1796, 2237, 2270
BmrI	ACTGGG	1 cut(s)	1798
BmsI	GCATC	7 cut(s)	376, 802, 907, 934, 1229, 1926, 2311
BmuI	ACTGGG	1 cut(s)	1798
BpmI	CTGGAG	2 cut(s)	1566, 2219
Bpu14I	TTCGAA	1 cut(s)	2527
BpuEI	CTTGAG	2 cut(s)	1330, 2488
BpuMI	CCSGG	1 cut(s)	105
Bsa29I	ATCGAT	2 cut(s)	370, 420
BsaBI	GATNNNNATC	2 cut(s)	567, 1584
BsaHI	GRCGYC	2 cut(s)	537, 2086
BsaI	GGTCTC	2 cut(s)	1348, 1767
BsaJI	CCNNGG	4 cut(s)	103, 488, 666, 1543
BsaWI	WCCGGW	2 cut(s)	182, 1178
BsaXI	ACNNNNNCTCC	2 cut(s)	2466, 2496
Bsc4I	CCNNNNNNNGG	6 cut(s)	407, 460, 1249, 1322, 1817, 2242
Bse118I	RCCGGY	1 cut(s)	182
Bse1I	ACTGG	7 cut(s)	172, 288, 1078, 1661, 1793, 2159, 2427
Bse8I	GATNNNNATC	2 cut(s)	567, 1584
BseBI	CCWGG	7 cut(s)	203, 838, 1437, 1545, 1796, 2237, 2270
BseCI	ATCGAT	2 cut(s)	370, 420
BseDI	CCNNGG	4 cut(s)	103, 488, 666, 1543
BseGI	GGATG	5 cut(s)	158, 309, 1118, 1132, 1585
BseJI	GATNNNNATC	2 cut(s)	567, 1584
BseLI	CCNNNNNNNGG	6 cut(s)	407, 460, 1249, 1322, 1817, 2242
BseMII	CTCAG	5 cut(s)	456, 1011, 1623, 2042, 2439
BseNI	ACTGG	7 cut(s)	172, 288, 1078, 1661, 1793, 2159, 2427
BseXI	GCAGC	7 cut(s)	15, 274, 310, 864, 979, 1197, 1339
BsgI	GTGCAG	1 cut(s)	464
Bsh1236I	CGCG	2 cut(s)	597, 1752
BshFI	GGCC	5 cut(s)	279, 841, 895, 1435, 1654
BshNI	GGYRCC	1 cut(s)	185
BshTI	ACCGGT	1 cut(s)	182
BshVI	ATCGAT	2 cut(s)	370, 420
BsiSI	CCGG	3 cut(s)	105, 183, 1179
BslFI	GGGAC	2 cut(s)	86, 1805
BslI	CCNNNNNNNGG	6 cut(s)	407, 460, 1249, 1322, 1817, 2242
BsmAI	GTCTC	5 cut(s)	243, 1348, 1375, 1767, 2413
BsmFI	GGGAC	2 cut(s)	86, 1805
BsmI	GAATGC	1 cut(s)	482
BsnI	GGCC	5 cut(s)	279, 841, 895, 1435, 1654
Bso31I	GGTCTC	2 cut(s)	1348, 1767
Bsp119I	TTCGAA	1 cut(s)	2527
Bsp1407I	TGTACA	3 cut(s)	553, 1392, 1611
Bsp19I	CCATGG	1 cut(s)	666
BspACI	CCGC	5 cut(s)	157, 461, 1208, 1589, 1777
BspANI	GGCC	5 cut(s)	279, 841, 895, 1435, 1654
BspCNI	CTCAG	5 cut(s)	457, 1012, 1624, 2043, 2440
BspDI	ATCGAT	2 cut(s)	370, 420
BspFNI	CGCG	2 cut(s)	597, 1752
BspHI	TCATGA	1 cut(s)	940
BspLI	GGNNCC	4 cut(s)	187, 1793, 1906, 2193
BspPI	GGATC	7 cut(s)	238, 1899, 1912, 2054, 2186, 2199, 2480
BspQI	GCTCTTC	1 cut(s)	2449
BspT104I	TTCGAA	1 cut(s)	2527
BspT107I	GGYRCC	1 cut(s)	185
BspTNI	GGTCTC	2 cut(s)	1348, 1767
BsrFI	RCCGGY	1 cut(s)	182
BsrGI	TGTACA	3 cut(s)	553, 1392, 1611
BsrI	ACTGG	7 cut(s)	172, 288, 1078, 1661, 1793, 2159, 2427
BssAI	RCCGGY	1 cut(s)	182
BssECI	CCNNGG	4 cut(s)	103, 488, 666, 1543
BssNI	GRCGYC	2 cut(s)	537, 2086
BssSI	CACGAG	1 cut(s)	32
BssT1I	CCWWGG	2 cut(s)	488, 666
Bst2BI	CACGAG	1 cut(s)	32
Bst2UI	CCWGG	7 cut(s)	203, 838, 1437, 1545, 1796, 2237, 2270
Bst4CI	ACNGT	7 cut(s)	166, 256, 1165, 1312, 1390, 1574, 1999
Bst6I	CTCTTC	3 cut(s)	2286, 2411, 2449
BstACI	GRCGYC	2 cut(s)	537, 2086
BstAPI	GCANNNNNTGC	1 cut(s)	1077
BstAUI	TGTACA	3 cut(s)	553, 1392, 1611
BstBI	TTCGAA	1 cut(s)	2527
BstC8I	GCNNGC	7 cut(s)	311, 413, 447, 762, 1296, 1629, 1956
BstDEI	CTNAG	7 cut(s)	465, 1020, 1632, 1695, 2051, 2079, 2448
BstDSI	CCRYGG	1 cut(s)	666
BstENI	CCTNNNNNAGG	1 cut(s)	1815
BstF5I	GGATG	5 cut(s)	158, 309, 1118, 1132, 1585
BstFNI	CGCG	2 cut(s)	597, 1752
BstHHI	GCGC	2 cut(s)	1752, 2275
BstMAI	GTCTC	5 cut(s)	243, 1348, 1375, 1767, 2413
BstMWI	GCNNNNNNNGC	4 cut(s)	11, 821, 1077, 1304
BstNI	CCWGG	7 cut(s)	203, 838, 1437, 1545, 1796, 2237, 2270
BstNSI	RCATGY	4 cut(s)	313, 764, 974, 1631
BstSCI	CCNGG	8 cut(s)	103, 201, 836, 1435, 1543, 1794, 2235, 2268
BstSFI	CTRYAG	2 cut(s)	1647, 1896
BstUI	CGCG	2 cut(s)	597, 1752
BstV1I	GCAGC	7 cut(s)	15, 274, 310, 864, 979, 1197, 1339
BstX2I	RGATCY	3 cut(s)	1892, 1904, 2191
BstXI	CCANNNNNNTGG	2 cut(s)	73, 1915
BstYI	RGATCY	3 cut(s)	1892, 1904, 2191
Bsu15I	ATCGAT	2 cut(s)	370, 420
BsuRI	GGCC	5 cut(s)	279, 841, 895, 1435, 1654
BsuTUI	ATCGAT	2 cut(s)	370, 420
BtgI	CCRYGG	1 cut(s)	666
BtsCI	GGATG	5 cut(s)	158, 309, 1118, 1132, 1585
BtsIMutI	CAGTG	6 cut(s)	528, 762, 1071, 1156, 1161, 1395
Cac8I	GCNNGC	7 cut(s)	311, 413, 447, 762, 1296, 1629, 1956
CaiI	CAGNNNCTG	2 cut(s)	1310, 2242
CciI	TCATGA	1 cut(s)	940
CfoI	GCGC	2 cut(s)	1752, 2275
Cfr10I	RCCGGY	1 cut(s)	182
Cfr13I	GGNCC	7 cut(s)	663, 893, 1433, 1652, 1792, 2456, 2477
ClaI	ATCGAT	2 cut(s)	370, 420
CseI	GACGC	2 cut(s)	78, 2075
Csp6I	GTAC	9 cut(s)	38, 175, 554, 645, 1313, 1393, 1486, 1612, 2044
CspAI	ACCGGT	1 cut(s)	182
CspCI	CAANNNNNGTGG	4 cut(s)	203, 238, 2481, 2516
CviQI	GTAC	9 cut(s)	38, 175, 554, 645, 1313, 1393, 1486, 1612, 2044
DdeI	CTNAG	7 cut(s)	465, 1020, 1632, 1695, 2051, 2079, 2448
DraIII	CACNNNGTG	1 cut(s)	1274
EaeI	YGGCCR	2 cut(s)	277, 839
Eam1104I	CTCTTC	3 cut(s)	2286, 2411, 2449
EarI	CTCTTC	3 cut(s)	2286, 2411, 2449
EciI	GGCGGA	1 cut(s)	172
Eco130I	CCWWGG	2 cut(s)	488, 666
Eco31I	GGTCTC	2 cut(s)	1348, 1767
Eco47I	GGWCC	4 cut(s)	663, 1792, 2456, 2477
Eco57I	CTGAAG	2 cut(s)	636, 1359
EcoNI	CCTNNNNNAGG	1 cut(s)	1815
EcoO109I	RGGNCCY	4 cut(s)	893, 1652, 1792, 2477
EcoRI	GAATTC	2 cut(s)	287, 1767
EcoRII	CCWGG	7 cut(s)	201, 836, 1435, 1543, 1794, 2235, 2268
EcoT14I	CCWWGG	2 cut(s)	488, 666
EcoT22I	ATGCAT	1 cut(s)	1222
ErhI	CCWWGG	2 cut(s)	488, 666
FaqI	GGGAC	2 cut(s)	86, 1805
FbaI	TGATCA	1 cut(s)	349
Fnu4HI	GCNGC	8 cut(s)	29, 263, 324, 462, 853, 968, 1211, 1353
FokI	GGATG	5 cut(s)	165, 316, 1125, 1139, 1592
Fsp4HI	GCNGC	8 cut(s)	29, 263, 324, 462, 853, 968, 1211, 1353
FspBI	CTAG	7 cut(s)	266, 362, 489, 963, 1605, 2498, 2548
GlaI	GCGC	2 cut(s)	1751, 2274
GluI	GCNGC	8 cut(s)	29, 263, 324, 462, 853, 968, 1211, 1353
GsuI	CTGGAG	2 cut(s)	1566, 2219
HaeIII	GGCC	5 cut(s)	279, 841, 895, 1435, 1654
HapII	CCGG	3 cut(s)	105, 183, 1179
HgaI	GACGC	2 cut(s)	78, 2075
HhaI	GCGC	2 cut(s)	1752, 2275
Hin1I	GRCGYC	2 cut(s)	537, 2086
Hin6I	GCGC	2 cut(s)	1750, 2273
HinP1I	GCGC	2 cut(s)	1750, 2273
HincII	GTYRAC	1 cut(s)	169
HindII	GTYRAC	1 cut(s)	169
HindIII	AAGCTT	2 cut(s)	12, 110
HpaII	CCGG	3 cut(s)	105, 183, 1179
HphI	GGTGA	3 cut(s)	587, 1586, 2515
Hpy166II	GTNNAC	7 cut(s)	169, 663, 1393, 1488, 1612, 1804, 1918
Hpy188I	TCNGA	9 cut(s)	152, 248, 354, 511, 616, 714, 1378, 1833, 2449
Hpy188III	TCNNGA	7 cut(s)	847, 941, 1062, 1461, 1772, 2102, 2184
Hpy8I	GTNNAC	7 cut(s)	169, 663, 1393, 1488, 1612, 1804, 1918
Hpy99I	CGWCG	2 cut(s)	94, 2091
HpyAV	CCTTC	6 cut(s)	516, 1018, 2140, 2205, 2394, 2447
HpyCH4III	ACNGT	7 cut(s)	166, 256, 1165, 1312, 1390, 1574, 1999
HpyCH4IV	ACGT	2 cut(s)	117, 537
HpyF10VI	GCNNNNNNNGC	4 cut(s)	11, 821, 1077, 1304
HpyF3I	CTNAG	7 cut(s)	465, 1020, 1632, 1695, 2051, 2079, 2448
HpySE526I	ACGT	2 cut(s)	117, 537
Hsp92I	GRCGYC	2 cut(s)	537, 2086
HspAI	GCGC	2 cut(s)	1750, 2273
Ksp22I	TGATCA	1 cut(s)	349
LguI	GCTCTTC	1 cut(s)	2449
LmnI	GCTCC	2 cut(s)	2239, 2518
Lsp1109I	GCAGC	7 cut(s)	15, 274, 310, 864, 979, 1197, 1339
LweI	GCATC	7 cut(s)	376, 802, 907, 934, 1229, 1926, 2311
MaeI	CTAG	7 cut(s)	266, 362, 489, 963, 1605, 2498, 2548
MaeII	ACGT	2 cut(s)	117, 537
MaeIII	GTNAC	7 cut(s)	394, 575, 1159, 1276, 1600, 1690, 2170
MboII	GAAGA	7 cut(s)	429, 2258, 2303, 2362, 2365, 2428, 2436
MfeI	CAATTG	3 cut(s)	861, 927, 1925
MflI	RGATCY	3 cut(s)	1892, 1904, 2191
MlsI	TGGCCA	2 cut(s)	279, 841
MluNI	TGGCCA	2 cut(s)	279, 841
MlyI	GAGTC	4 cut(s)	352, 391, 842, 1166
MmeI	TCCRAC	2 cut(s)	130, 2513
Mox20I	TGGCCA	2 cut(s)	279, 841
Mph1103I	ATGCAT	1 cut(s)	1222
MscI	TGGCCA	2 cut(s)	279, 841
MseI	TTAA	7 cut(s)	114, 378, 600, 873, 1215, 1248, 1836
MslI	CAYNNNNRTG	7 cut(s)	138, 144, 341, 765, 942, 1337, 1607
Msp20I	TGGCCA	2 cut(s)	279, 841
MspA1I	CMGCKG	1 cut(s)	1210
MspI	CCGG	3 cut(s)	105, 183, 1179
MspR9I	CCNGG	8 cut(s)	105, 203, 838, 1437, 1545, 1796, 2237, 2270
MunI	CAATTG	3 cut(s)	861, 927, 1925
Mva1269I	GAATGC	1 cut(s)	482
MvaI	CCWGG	7 cut(s)	203, 838, 1437, 1545, 1796, 2237, 2270
MvnI	CGCG	2 cut(s)	597, 1752
MwoI	GCNNNNNNNGC	4 cut(s)	11, 821, 1077, 1304
NciI	CCSGG	1 cut(s)	105
NcoI	CCATGG	1 cut(s)	666
NlaIV	GGNNCC	4 cut(s)	187, 1793, 1906, 2193
NmuCI	GTSAC	6 cut(s)	394, 575, 1159, 1276, 1600, 2170
NsiI	ATGCAT	1 cut(s)	1222
NspI	RCATGY	4 cut(s)	313, 764, 974, 1631
NspV	TTCGAA	1 cut(s)	2527
OliI	CACNNNNGTG	1 cut(s)	1607
PaeI	GCATGC	3 cut(s)	313, 764, 1631
PagI	TCATGA	1 cut(s)	940
PciSI	GCTCTTC	1 cut(s)	2449
PctI	GAATGC	1 cut(s)	482
PflFI	GACNNNGTC	2 cut(s)	146, 254
PflMI	CCANNNNNTGG	3 cut(s)	407, 1322, 2242
PfoI	TCCNGGA	1 cut(s)	2235
PinAI	ACCGGT	1 cut(s)	182
PkrI	GCNGC	8 cut(s)	30, 264, 325, 463, 854, 969, 1212, 1354
PleI	GAGTC	4 cut(s)	352, 391, 841, 1166
PpsI	GAGTC	4 cut(s)	352, 391, 841, 1166
PpuMI	RGGWCCY	2 cut(s)	1792, 2477
PshBI	ATTAAT	1 cut(s)	1836
PsiI	TTATAA	2 cut(s)	1011, 2228
Psp1406I	AACGTT	1 cut(s)	117
Psp5II	RGGWCCY	2 cut(s)	1792, 2477
Psp6I	CCWGG	7 cut(s)	201, 836, 1435, 1543, 1794, 2235, 2268
PspGI	CCWGG	7 cut(s)	201, 836, 1435, 1543, 1794, 2235, 2268
PspN4I	GGNNCC	4 cut(s)	187, 1793, 1906, 2193
PspPI	GGNCC	7 cut(s)	663, 893, 1433, 1652, 1792, 2456, 2477
PspPPI	RGGWCCY	2 cut(s)	1792, 2477
PstNI	CAGNNNCTG	2 cut(s)	1310, 2242
PsuI	RGATCY	3 cut(s)	1892, 1904, 2191
PsyI	GACNNNGTC	2 cut(s)	146, 254
RsaI	GTAC	9 cut(s)	39, 176, 555, 646, 1314, 1394, 1487, 1613, 2045
RsaNI	GTAC	9 cut(s)	38, 175, 554, 645, 1313, 1393, 1486, 1612, 2044
RseI	CAYNNNNRTG	7 cut(s)	138, 144, 341, 765, 942, 1337, 1607
SapI	GCTCTTC	1 cut(s)	2449
SaqAI	TTAA	7 cut(s)	114, 378, 600, 873, 1215, 1248, 1836
SatI	GCNGC	8 cut(s)	29, 263, 324, 462, 853, 968, 1211, 1353
Sau96I	GGNCC	7 cut(s)	663, 893, 1433, 1652, 1792, 2456, 2477
ScaI	AGTACT	1 cut(s)	176
SchI	GAGTC	4 cut(s)	352, 391, 842, 1166
ScrFI	CCNGG	8 cut(s)	105, 203, 838, 1437, 1545, 1796, 2237, 2270
SfaNI	GCATC	7 cut(s)	376, 802, 907, 934, 1229, 1926, 2311
SfcI	CTRYAG	2 cut(s)	1647, 1896
SfuI	TTCGAA	1 cut(s)	2527
SgrAI	CRCCGGYG	1 cut(s)	182
SinI	GGWCC	4 cut(s)	663, 1792, 2456, 2477
SmiMI	CAYNNNNRTG	7 cut(s)	138, 144, 341, 765, 942, 1337, 1607
SmlI	CTYRAG	2 cut(s)	1345, 2467
SmoI	CTYRAG	2 cut(s)	1345, 2467
SphI	GCATGC	3 cut(s)	313, 764, 1631
SsiI	CCGC	5 cut(s)	157, 461, 1208, 1589, 1777
SspMI	CTAG	7 cut(s)	266, 362, 489, 963, 1605, 2498, 2548
StyD4I	CCNGG	8 cut(s)	103, 201, 836, 1435, 1543, 1794, 2235, 2268
StyI	CCWWGG	2 cut(s)	488, 666
TaaI	ACNGT	7 cut(s)	166, 256, 1165, 1312, 1390, 1574, 1999
TaiI	ACGT	2 cut(s)	120, 540
TaqI	TCGA	6 cut(s)	370, 420, 1771, 2089, 2527, 2541
TaqII	GACCGA	2 cut(s)	1495, 2473
TatI	WGTACW	5 cut(s)	174, 553, 1392, 1485, 1611
TauI	GCSGC	1 cut(s)	464
Tru1I	TTAA	7 cut(s)	114, 378, 600, 873, 1215, 1248, 1836
Tru9I	TTAA	7 cut(s)	114, 378, 600, 873, 1215, 1248, 1836
TscAI	CASTG	6 cut(s)	535, 762, 1078, 1156, 1168, 1395
TseFI	GTSAC	6 cut(s)	394, 575, 1159, 1276, 1600, 2170
TseI	GCWGC	7 cut(s)	28, 262, 323, 852, 967, 1210, 1352
Tsp45I	GTSAC	6 cut(s)	394, 575, 1159, 1276, 1600, 2170
TspRI	CASTG	6 cut(s)	535, 762, 1078, 1156, 1168, 1395
Tth111I	GACNNNGTC	2 cut(s)	146, 254
Van91I	CCANNNNNTGG	3 cut(s)	407, 1322, 2242
VpaK11BI	GGWCC	4 cut(s)	663, 1792, 2456, 2477
VspI	ATTAAT	1 cut(s)	1836
XagI	CCTNNNNNAGG	1 cut(s)	1815
XapI	RAATTY	6 cut(s)	287, 722, 867, 1640, 1767, 2523
XceI	RCATGY	4 cut(s)	313, 764, 974, 1631
XcmI	CCANNNNNNNNNTGG	1 cut(s)	408
XmaJI	CCTAGG	1 cut(s)	488
XspI	CTAG	7 cut(s)	266, 362, 489, 963, 1605, 2498, 2548
ZraI	GACGTC	1 cut(s)	538
ZrmI	AGTACT	1 cut(s)	176
Zsp2I	ATGCAT	1 cut(s)	1222

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.