Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0001301

Physical Location & Seq

Genomic Coordinates Forward (+)

303551 .. 311619

Gene Structure 8069 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1002900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3123 bp

ATGGCGGCGACTCCGTCCCTCTTGTGCTTGCTCACGTTGCTCGTCGGATTTCTAGTCGCCGACTCCAAGTTTATTGCATACAACACTTCCCAAGGCATAGTCCCCGGCAAAATCAATGTCCATTTGGTCCCTCACACCCACGACGACGTTGGCTGGTTAAAGACCGTTGATCAGTATTATGTTGGCTCCAACAATTCCATCCAGGGGGCTTGCGTGCAAAATGTGTTGGATTCTTTGGTTCCTGCTCTGCTGGCTGACAAGAATCGCAAATTCATTTATGCATTCTTCCAGCGGTGGTGGAGAGATCAGAGTGACTCCGTCCAGAGTATAGTCAAGCAGCTAGTCACCTCGGGTCAATTAGAGTTCATAAATGGGGGAATGTGCATGCATGATGAGGCTGCAACACATTATATTGATATGATAGATCAGACGACTCTAGGGCATCAGTTTATTAAAAGGGAATTTGATGTGACTCCAAGAATTGGTTGGCAAATTGACCCATTTGGGCATTCGGCTGTCCAGGCTTACTTGTTGGGAGCAGAAGTTGGATTTGACTCACTTTTCTTCGGGCGAATAGATTACCAGGACAGGGCTAAGCGCAAAATTGAGAAGAGCCTTGAATTTGTTTGGCGGGGTTCGAAGAGTCTCAGTTCATCTGCTCAGATTTTTTCTGGTGCATTCCCAGAGAACTATGAGCCTCCATCTGGTTTCTACTTTGAAGTTAATGATAATTCCCCAATTGTTCAGGATGACATCACTTTGTTTGATTACAATGTTCAAGATCGTGTTAATGACTTTGTTGCTGCTGCAGTAGCACAGGCTAACATAACTCGAACAAATCATATAATGTGGACCATGGGAACAGATTTCAAGTATCAGTATGCACACACATGGTTCCGACAAATGGACAAGCTCATTCATTATGTCAACAAGGATGGACGTGTTAATGCTCTGTATTCTACTCCATCCATATATACGGATGCAAAGTATGCTACAAATGAATCATGGCCAATCAAGACAGATGATTTCTTTCCTTATGCAGATCGTACAAATGCTTACTGGACAGGGTACTTCACAAGTAGACCAGCCTTGAAACATTACGTTAGAGCAATGAGTGGCTATTATTTGGCTGCCAGGCAACTAGAATATTTTAAAGGAAGGACTGGTTCAGGGCCCAACACAGACTCGTTGGCAGATGCCTTAGCAATTGCTCAACATCATGATGCAGTCACTGGTACAGAGAAGCAGCATGTTGCCAATGATTATGCAAAAAGGTTGGCGATAGGCTACACGGAGGCCGAGCAAGTAGTTGCCACGTCACTTGCTCACTTGGTTGAGTCTGCATCATACACTGGATTTGTAGATCCAACTACAAAGTTTCAACAGTGCCCACTTCTAAATATCAGCTACTGCCCTGCATCAGAAGTGAATTTATCCCAAGGGAAGCAATTGGTTGTCATGGTGTACAATTCCCTTGGATGGAAGAGGGATGATGTAATTCGAATTCCTGTTATCAATGAAGATGTCACTGTTCATGATTCTGAGGGAAGAGAAATTGAATCACAGCTTCTCCCACTAGATGATGCCCATGTGGGCTTAAGGAACTACTATGCTAAGGCATACTTGGGTCAAACACCAACAAAGACACCAAACTATTGGCTTGCATTTACAGTATCCGTGCCACCACTTGGTTTCAGCACATACACTATCTCAGCTGCCAAAGGAGCAGGTGCTGGTTCCACCAGATCATCTGTACAGACATTTCAAAGTAGGGAAGAGTCCACTATTGAAGTTGGTCAAGGAAATGTGAAACTCACGTTTTCTACAAATCAAGGCAAACTGACCAATTATGTCAATAGAAGAAGCTTGGTAAGTAATGCACCTAACAGTCGCGTAGTTACTATACCTGATGATTGCATTTCCTTATCCACCAAATTGGTCGAACAGTCTTACAGTTTTTATAACGGATATAATGGCAGTGATGATAAAGCTCCTCTGATTCCTCAGAATGCTGGGGCTTATATCTTCCGTCCAAATGGCACGTTTCTCATAAAACCTGGAGAAAAGGCTTCTCTGACTGTCGTGCGTGGACCAGTAATAGATGAAGTACATCAACATATTAATTCATGGATATATCAGGTCACCAGAATTCACAAAGAGAAGGAGCATGTTGAAGTCGAGTTTATTGTTGGGCCATTACCTACTGATGACGGAATTGGAAAAGAAGTTGTAACTCAACTGGCCACTACCATGGCTACCAACAAAACGTTCTACACTGATTCTAATGGGCGTGATTTTATTAAAAGGATTCGCGACTATAGAACAGATTGGGACCTGAAAGTGCACCAACCTATAGCTGGAAATTATTATCCAATTAATCTTGGGATATACATGCAAGATATTAGTACAGAATTTTCGGTTTTGGTGGACCGATCTGTAGGAGGGTCCAGCACAGTGGATGGACAGATAGAGTTGATGCTCCACAGGAGACTACTGCTTGATGACTCAAGAGGTGTGGCGGAGGCTCTAAATGAAACTGTCTGTATTGACAACGTTTGCAGTGGACTAAGAATCCAAGGAAAATTTTATTTCAGAATCGATCCCCTGGGAGAAGGTGCCAAGTGGCGTCGTACTTTCGGTCAGGAGATATATTCCCCTCTTTTACTAGCTTTTTCTGAACAGGATGGAGATAACCGGAAGAATTCTCATGTAACCACGTTTTCAGGGGTTGGTTCCTCATACAGTTTACCTGATAATGTCGCTCTTATAACTCTTCAGGAGCTTGATGATGGAAAAGTTCTCCTCCGATTAGCACATTTATATGAGATTGGAGAGGACAGGGATCTTTCTGTAATGACAAATGTAGAGTTGAAGCAGCTCTTCCCCAAGAAGAAGATCGGTAAACTTACAGAGATGAACTTGTCGGCAAATCAAGAACGGACAGAAATGGAGAAAAAGAGACTCAATTGGAAGGTGGAAGAAGGCTCTTCATCAGAAGCAAAGGTTTCGAGGGGTGGACCTGTTGATCCTACGAAACTGGTTGTGGAACTTGCACCAATGGAAATCCGAACCTTTCTTGTTGAGTTTAACGAAAGCTTCCATCGTTATTTATTTGATGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0001775 GO:0002252 GO:0002263 GO:0002274 GO:0002275 GO:0002283 GO:0002366 GO:0002376 GO:0002443 GO:0002444 GO:0002446 GO:0003674 GO:0003824 GO:0004553 GO:0004559 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005766 GO:0005773 GO:0005775 GO:0005975 GO:0005996 GO:0006013 GO:0006464 GO:0006517 GO:0006807 GO:0006810 GO:0006887 GO:0006955 GO:0008150 GO:0008152 GO:0009056 GO:0009100 GO:0009311 GO:0009313 GO:0009987 GO:0012505 GO:0015923 GO:0016052 GO:0016192 GO:0016787 GO:0016798 GO:0019318 GO:0019538 GO:0030141 GO:0031410 GO:0031974 GO:0031982 GO:0031983 GO:0032940 GO:0034774 GO:0035578 GO:0036211 GO:0036230 GO:0042119 GO:0042582 GO:0043170 GO:0043202 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043299 GO:0043312 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044281 GO:0044421 GO:0044422 GO:0044424 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045055 GO:0045321 GO:0046903 GO:0050896 GO:0051179 GO:0051234 GO:0060205 GO:0070013 GO:0071704 GO:0097708 GO:0099503 GO:1901135 GO:1901564 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Alpha-mann_mid Glyco_hydro_38 Glyco_hydro_38C

Protein Analysis

1041

Amino Acids

116.86

Weight (kDa)

5.85

Isoelectric Point (pI)

36.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	39 - 347	1.2e-79	Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid	PF09261	352 - 444	3.8e-21	Alpha mannosidase middle domain
Laman-like_dom	PF21260	519 - 570	4e-10	Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C	PF07748	647 - 837	6.1e-41	Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2	PF17677	919 - 1025	5.5e-08	Glycosyl hydrolases family 38 C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001301)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13980 AT5G13980 AT5G13980
fragaria_vesca	FvH4_5g09420 FvH4_5g09421 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272
malus_domestica	MD06G1131600.v1.1 MD09G1002900.v1.1 MD17G1007000.v1.1
prunus_persica	Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.5G142600_v2.0.a1 Prupe.5G142600_v2.0.a1
pyrus_communis	pycom06g12300 pycom06g12310 pycom111g00240 pycom17g00260
rosa_chinensis	RchiOBHm_Chr2g0176471 RchiOBHm_Chr2g0176481 RchiOBHm_Chr7g0188771 RchiOBHm_Chr7g0188781
rosa_laevigata	RLG00000004661 RLG00000004662 RLG00000022416
rosa_multiflora	Rmu_sc0000303.1_g000057 Rmu_sc0003642.1_g000008 Rmu_sc0011904.1_g000012
rosa_roxburghii	Rroxscaffold_2G00076370 Rroxscaffold_3G00266040 Rroxscaffold_3G00266050
rosa_rugosa	Rorug02G0595000 Rorug06G0493700
rosa_samantha	Rh2AG673300 Rh2BG684600 Rh2CG647400 Rh2DG698200 Rh7AG098300 Rh7AG098400 Rh7BG099900 Rh7BG100200 Rh7CG101600 Rh7CG101700 Rh7DG100100 Rh7DG100200
rosa_wichuraiana	Rw0G010390 Rw2G055220 Rw7G008480 Rw7G008490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1962, 2768
AarI	CACCTGC	1 cut(s)	1718
Acc36I	ACCTGC	1 cut(s)	1718
AccB1I	GGYRCC	1 cut(s)	2615
AccB7I	CCANNNNNTGG	2 cut(s)	482, 1688
AccI	GTMKAC	1 cut(s)	1081
AccII	CGCG	2 cut(s)	1893, 2313
AciI	CCGC	4 cut(s)	5, 292, 631, 2519
AclI	AACGTT	2 cut(s)	2267, 2553
AclWI	GGATC	4 cut(s)	1358, 2594, 2850, 3020
AcoI	YGGCCR	2 cut(s)	1007, 2241
AcsI	RAATTY	9 cut(s)	269, 461, 620, 1429, 1503, 2148, 2411, 2582, 2701
AcuI	CTGAAG	1 cut(s)	2759
AcyI	GRCGYC	1 cut(s)	2626
AfaI	GTAC	8 cut(s)	1048, 1070, 1237, 1466, 1755, 2109, 2407, 2632
AfiI	CCNNNNNNNGG	8 cut(s)	204, 482, 589, 704, 904, 1479, 1688, 2357
AflII	CTTAAG	1 cut(s)	1597
AflIII	ACRYGT	1 cut(s)	940
AjiI	CACGTC	2 cut(s)	941, 1317
AjnI	CCWGG	6 cut(s)	201, 519, 582, 1133, 2056, 2604
AjuI	GAANNNNNNNTTGG	2 cut(s)	2252, 2284
Alw21I	GWGCWC	1 cut(s)	2346
Alw26I	GTCTC	3 cut(s)	650, 2482, 2953
Alw44I	GTGCAC	1 cut(s)	2342
AlwI	GGATC	4 cut(s)	1358, 2594, 2850, 3020
AlwNI	CAGNNNCTG	3 cut(s)	1232, 1410, 1733
Ama87I	CYCGRG	1 cut(s)	349
AoxI	GGCC	5 cut(s)	1007, 1172, 1296, 2192, 2241
ApaI	GGGCCC	1 cut(s)	1176
ApaLI	GTGCAC	1 cut(s)	2342
ApeKI	GCWGC	8 cut(s)	337, 398, 803, 806, 1130, 1246, 1715, 2875
ApoI	RAATTY	9 cut(s)	269, 461, 620, 1429, 1503, 2148, 2411, 2582, 2701
ArsI	GACNNNNNNTTYG	2 cut(s)	102, 134
AseI	ATTAAT	2 cut(s)	2121, 2376
AspLEI	GCGC	1 cut(s)	600
AsuC2I	CCSGG	1 cut(s)	105
AsuHPI	GGTGA	2 cut(s)	337, 2134
AsuII	TTCGAA	2 cut(s)	638, 1501
AvaI	CYCGRG	1 cut(s)	349
AvaII	GGWCC	7 cut(s)	127, 852, 2090, 2332, 2428, 2445, 3017
BaeGI	GKGCMC	3 cut(s)	1176, 1391, 2346
BalI	TGGCCA	2 cut(s)	1009, 2243
BanI	GGYRCC	1 cut(s)	2615
BanII	GRGCYC	1 cut(s)	1176
Bbv12I	GWGCWC	1 cut(s)	2346
BbvI	GCAGC	8 cut(s)	349, 385, 790, 793, 1117, 1258, 1702, 2887
BccI	CCATC	9 cut(s)	206, 709, 929, 973, 1473, 2453, 2678, 2783, 3108
BcgI	CGANNNNNNTGC	2 cut(s)	2988, 3022
BciT130I	CCWGG	6 cut(s)	203, 521, 584, 1135, 2058, 2606
BciVI	GTATCC	1 cut(s)	1684
BclI	TGATCA	1 cut(s)	169
BcnI	CCSGG	1 cut(s)	105
BcoDI	GTCTC	3 cut(s)	650, 2482, 2953
BfaI	CTAG	6 cut(s)	53, 341, 437, 1142, 1577, 2666
BfmI	CTRYAG	4 cut(s)	807, 2317, 2352, 2436
BfrI	CTTAAG	1 cut(s)	1597
BfuAI	ACCTGC	1 cut(s)	1718
BfuI	GTATCC	1 cut(s)	1684
BisI	GCNGC	9 cut(s)	6, 338, 399, 804, 807, 1131, 1247, 1716, 2876
BlpI	GCTNAGC	1 cut(s)	594
BlsI	GCNGC	9 cut(s)	7, 339, 400, 805, 808, 1132, 1248, 1717, 2877
Bme1390I	CCNGG	7 cut(s)	105, 203, 521, 584, 1135, 2058, 2606
Bme18I	GGWCC	7 cut(s)	127, 852, 2090, 2332, 2428, 2445, 3017
BmeT110I	CYCGRG	1 cut(s)	349
BmgBI	CACGTC	2 cut(s)	941, 1317
BmrFI	CCNGG	7 cut(s)	105, 203, 521, 584, 1135, 2058, 2606
BmsI	GCATC	9 cut(s)	451, 970, 1186, 1213, 1352, 1427, 1573, 2466, 3106
BpmI	CTGGAG	1 cut(s)	2079
Bpu10I	CCTNAGC	2 cut(s)	1201, 1614
Bpu1102I	GCTNAGC	1 cut(s)	594
Bpu14I	TTCGAA	2 cut(s)	638, 1501
BpuEI	CTTGAG	1 cut(s)	2491
BpuMI	CCSGG	1 cut(s)	105
Bsa29I	ATCGAT	1 cut(s)	2598
BsaHI	GRCGYC	1 cut(s)	2626
BsaWI	WCCGGW	1 cut(s)	2694
BsaXI	ACNNNNNCTCC	4 cut(s)	528, 558, 1554, 1584
Bsc4I	CCNNNNNNNGG	8 cut(s)	204, 482, 589, 704, 904, 1479, 1688, 2357
Bse1I	ACTGG	7 cut(s)	1064, 1168, 1237, 1357, 2093, 2244, 3042
Bse3DI	GCAATG	1 cut(s)	1116
BseBI	CCWGG	6 cut(s)	203, 521, 584, 1135, 2058, 2606
BseCI	ATCGAT	1 cut(s)	2598
BseGI	GGATG	9 cut(s)	198, 754, 940, 965, 985, 1484, 1495, 2464, 2689
BseLI	CCNNNNNNNGG	8 cut(s)	204, 482, 589, 704, 904, 1479, 1688, 2357
BseMI	GCAATG	1 cut(s)	1116
BseMII	CTCAG	5 cut(s)	661, 674, 1533, 1725, 2018
BseNI	ACTGG	7 cut(s)	1064, 1168, 1237, 1357, 2093, 2244, 3042
BseRI	GAGGAG	2 cut(s)	1983, 2792
BseSI	GKGCMC	3 cut(s)	1176, 1391, 2346
BseXI	GCAGC	8 cut(s)	349, 385, 790, 793, 1117, 1258, 1702, 2887
BseYI	CCCAGC	1 cut(s)	2012
Bsh1236I	CGCG	2 cut(s)	1893, 2313
BshFI	GGCC	5 cut(s)	1009, 1174, 1298, 2194, 2243
BshNI	GGYRCC	1 cut(s)	2615
BshVI	ATCGAT	1 cut(s)	2598
BsiHKAI	GWGCWC	1 cut(s)	2346
BsiHKCI	CYCGRG	1 cut(s)	349
BsiSI	CCGG	2 cut(s)	105, 2695
BslFI	GGGAC	3 cut(s)	86, 113, 2345
BslI	CCNNNNNNNGG	8 cut(s)	204, 482, 589, 704, 904, 1479, 1688, 2357
BsmAI	GTCTC	3 cut(s)	650, 2482, 2953
BsmFI	GGGAC	3 cut(s)	86, 113, 2345
BsmI	GAATGC	4 cut(s)	281, 508, 677, 2014
BsnI	GGCC	5 cut(s)	1009, 1174, 1298, 2194, 2243
BsoBI	CYCGRG	1 cut(s)	349
Bsp119I	TTCGAA	2 cut(s)	638, 1501
Bsp120I	GGGCCC	1 cut(s)	1172
Bsp1286I	GDGCHC	3 cut(s)	1176, 1391, 2346
Bsp1407I	TGTACA	2 cut(s)	1464, 1753
Bsp1720I	GCTNAGC	1 cut(s)	594
Bsp19I	CCATGG	2 cut(s)	855, 2250
Bsp68I	TCGCGA	1 cut(s)	2313
BspACI	CCGC	4 cut(s)	5, 292, 631, 2519
BspANI	GGCC	5 cut(s)	1009, 1174, 1298, 2194, 2243
BspCNI	CTCAG	5 cut(s)	660, 673, 1534, 1724, 2017
BspDI	ATCGAT	1 cut(s)	2598
BspFNI	CGCG	2 cut(s)	1893, 2313
BspHI	TCATGA	2 cut(s)	1219, 1534
BspMAI	CTGCAG	1 cut(s)	811
BspMI	ACCTGC	1 cut(s)	1718
BspPI	GGATC	4 cut(s)	1358, 2594, 2850, 3020
BspQI	GCTCTTC	3 cut(s)	605, 2885, 2992
BspT104I	TTCGAA	2 cut(s)	638, 1501
BspT107I	GGYRCC	1 cut(s)	2615
BspTI	CTTAAG	1 cut(s)	1597
BsrDI	GCAATG	1 cut(s)	1116
BsrGI	TGTACA	2 cut(s)	1464, 1753
BsrI	ACTGG	7 cut(s)	1064, 1168, 1237, 1357, 2093, 2244, 3042
BssNI	GRCGYC	1 cut(s)	2626
BssT1I	CCWWGG	6 cut(s)	91, 855, 1438, 1474, 2250, 2575
Bst2UI	CCWGG	6 cut(s)	203, 521, 584, 1135, 2058, 2606
Bst6I	CTCTTC	8 cut(s)	605, 635, 1478, 1543, 1770, 2778, 2885, 2992
BstACI	GRCGYC	1 cut(s)	2626
BstAFI	CTTAAG	1 cut(s)	1597
BstAPI	GCANNNNNTGC	1 cut(s)	989
BstAUI	TGTACA	2 cut(s)	1464, 1753
BstBI	TTCGAA	2 cut(s)	638, 1501
BstC8I	GCNNGC	6 cut(s)	29, 211, 215, 252, 386, 1662
BstDEI	CTNAG	9 cut(s)	594, 647, 660, 1201, 1542, 1614, 1711, 2004, 2567
BstDSI	CCRYGG	2 cut(s)	855, 2250
BstEII	GGTNACC	1 cut(s)	2140
BstF5I	GGATG	9 cut(s)	198, 754, 940, 965, 985, 1484, 1495, 2464, 2689
BstFNI	CGCG	2 cut(s)	1893, 2313
BstHHI	GCGC	1 cut(s)	600
BstMAI	GTCTC	3 cut(s)	650, 2482, 2953
BstMWI	GCNNNNNNNGC	6 cut(s)	37, 251, 521, 812, 989, 1724
BstNI	CCWGG	6 cut(s)	203, 521, 584, 1135, 2058, 2606
BstNSI	RCATGY	4 cut(s)	388, 1253, 2171, 2395
BstPI	GGTNACC	1 cut(s)	2140
BstSCI	CCNGG	7 cut(s)	103, 201, 519, 582, 1133, 2056, 2604
BstSFI	CTRYAG	4 cut(s)	807, 2317, 2352, 2436
BstSLI	GKGCMC	3 cut(s)	1176, 1391, 2346
BstUI	CGCG	2 cut(s)	1893, 2313
BstV1I	GCAGC	8 cut(s)	349, 385, 790, 793, 1117, 1258, 1702, 2887
BstX2I	RGATCY	2 cut(s)	1363, 2842
BstXI	CCANNNNNNTGG	4 cut(s)	1656, 1936, 2251, 2455
BstYI	RGATCY	2 cut(s)	1363, 2842
Bsu15I	ATCGAT	1 cut(s)	2598
BsuI	GTATCC	1 cut(s)	1684
BsuRI	GGCC	5 cut(s)	1009, 1174, 1298, 2194, 2243
BsuTUI	ATCGAT	1 cut(s)	2598
BtgI	CCRYGG	2 cut(s)	855, 2250
BtrI	CACGTC	2 cut(s)	941, 1317
BtsCI	GGATG	9 cut(s)	198, 754, 940, 965, 985, 1484, 1495, 2464, 2689
BtsI	GCAGTG	2 cut(s)	1984, 2566
BtsIMutI	CAGTG	8 cut(s)	1230, 1350, 1391, 1527, 1984, 2274, 2460, 2566
BtuMI	TCGCGA	1 cut(s)	2313
BveI	ACCTGC	1 cut(s)	1718
Cac8I	GCNNGC	6 cut(s)	29, 211, 215, 252, 386, 1662
CaiI	CAGNNNCTG	3 cut(s)	1232, 1410, 1733
CciI	TCATGA	2 cut(s)	1219, 1534
CfoI	GCGC	1 cut(s)	600
ClaI	ATCGAT	1 cut(s)	2598
CseI	GACGC	1 cut(s)	2615
Csp6I	GTAC	8 cut(s)	1047, 1069, 1236, 1465, 1754, 2108, 2406, 2631
CspCI	CAANNNNNGTGG	4 cut(s)	1303, 1338, 2496, 2531
CviQI	GTAC	8 cut(s)	1047, 1069, 1236, 1465, 1754, 2108, 2406, 2631
DdeI	CTNAG	9 cut(s)	594, 647, 660, 1201, 1542, 1614, 1711, 2004, 2567
DraI	TTTAAA	1 cut(s)	1153
EaeI	YGGCCR	2 cut(s)	1007, 2241
Eam1104I	CTCTTC	8 cut(s)	605, 635, 1478, 1543, 1770, 2778, 2885, 2992
EarI	CTCTTC	8 cut(s)	605, 635, 1478, 1543, 1770, 2778, 2885, 2992
EciI	GGCGGA	1 cut(s)	2534
Eco130I	CCWWGG	6 cut(s)	91, 855, 1438, 1474, 2250, 2575
Eco24I	GRGCYC	1 cut(s)	1176
Eco47I	GGWCC	7 cut(s)	127, 852, 2090, 2332, 2428, 2445, 3017
Eco57I	CTGAAG	1 cut(s)	2759
Eco88I	CYCGRG	1 cut(s)	349
Eco91I	GGTNACC	1 cut(s)	2140
EcoO109I	RGGNCCY	2 cut(s)	1172, 2332
EcoO65I	GGTNACC	1 cut(s)	2140
EcoRI	GAATTC	3 cut(s)	1503, 2148, 2701
EcoRII	CCWGG	6 cut(s)	201, 519, 582, 1133, 2056, 2604
EcoT14I	CCWWGG	6 cut(s)	91, 855, 1438, 1474, 2250, 2575
EcoT22I	ATGCAT	2 cut(s)	283, 390
EcoT38I	GRGCYC	1 cut(s)	1176
ErhI	CCWWGG	6 cut(s)	91, 855, 1438, 1474, 2250, 2575
FalI	AAGNNNNNCTT	4 cut(s)	1607, 1639, 2864, 2896
FaqI	GGGAC	3 cut(s)	86, 113, 2345
FauI	CCCGC	1 cut(s)	624
FbaI	TGATCA	1 cut(s)	169
FblI	GTMKAC	1 cut(s)	1081
Fnu4HI	GCNGC	9 cut(s)	6, 338, 399, 804, 807, 1131, 1247, 1716, 2876
FokI	GGATG	9 cut(s)	185, 761, 947, 952, 992, 1491, 1502, 2471, 2696
FriOI	GRGCYC	1 cut(s)	1176
Fsp4HI	GCNGC	9 cut(s)	6, 338, 399, 804, 807, 1131, 1247, 1716, 2876
FspBI	CTAG	6 cut(s)	53, 341, 437, 1142, 1577, 2666
GlaI	GCGC	1 cut(s)	599
GluI	GCNGC	9 cut(s)	6, 338, 399, 804, 807, 1131, 1247, 1716, 2876
GsaI	CCCAGC	1 cut(s)	2016
GsuI	CTGGAG	1 cut(s)	2079
HaeIII	GGCC	5 cut(s)	1009, 1174, 1298, 2194, 2243
HapII	CCGG	2 cut(s)	105, 2695
HgaI	GACGC	1 cut(s)	2615
HhaI	GCGC	1 cut(s)	600
Hin1I	GRCGYC	1 cut(s)	2626
Hin6I	GCGC	1 cut(s)	598
HinP1I	GCGC	1 cut(s)	598
HincII	GTYRAC	1 cut(s)	928
HindII	GTYRAC	1 cut(s)	928
HindIII	AAGCTT	2 cut(s)	1864, 3094
HpaII	CCGG	2 cut(s)	105, 2695
HphI	GGTGA	2 cut(s)	337, 2134
Hpy99I	CGWCG	4 cut(s)	47, 146, 149, 2631
HpyAV	CCTTC	5 cut(s)	1152, 2155, 2606, 2965, 2975
HpyF10VI	GCNNNNNNNGC	6 cut(s)	37, 251, 521, 812, 989, 1724
HpyF3I	CTNAG	9 cut(s)	594, 647, 660, 1201, 1542, 1614, 1711, 2004, 2567
Hsp92I	GRCGYC	1 cut(s)	2626
HspAI	GCGC	1 cut(s)	598
Ksp22I	TGATCA	1 cut(s)	169
LguI	GCTCTTC	3 cut(s)	605, 2885, 2992
LmnI	GCTCC	7 cut(s)	191, 536, 1724, 1996, 2164, 2484, 2779
Lsp1109I	GCAGC	8 cut(s)	349, 385, 790, 793, 1117, 1258, 1702, 2887
LweI	GCATC	9 cut(s)	451, 970, 1186, 1213, 1352, 1427, 1573, 2466, 3106
MaeI	CTAG	6 cut(s)	53, 341, 437, 1142, 1577, 2666
MfeI	CAATTG	4 cut(s)	738, 1206, 1448, 2965
MflI	RGATCY	2 cut(s)	1363, 2842
MhlI	GDGCHC	3 cut(s)	1176, 1391, 2346
MlsI	TGGCCA	2 cut(s)	1009, 2243
MluNI	TGGCCA	2 cut(s)	1009, 2243
MmeI	TCCRAC	6 cut(s)	25, 207, 213, 526, 922, 1391
Mox20I	TGGCCA	2 cut(s)	1009, 2243
Mph1103I	ATGCAT	2 cut(s)	283, 390
MscI	TGGCCA	2 cut(s)	1009, 2243
MslI	CAYNNNNRTG	4 cut(s)	889, 1221, 2249, 2820
Msp20I	TGGCCA	2 cut(s)	1009, 2243
MspA1I	CMGCKG	2 cut(s)	292, 1715
MspCI	CTTAAG	1 cut(s)	1597
MspI	CCGG	2 cut(s)	105, 2695
MspR9I	CCNGG	7 cut(s)	105, 203, 521, 584, 1135, 2058, 2606
MunI	CAATTG	4 cut(s)	738, 1206, 1448, 2965
Mva1269I	GAATGC	4 cut(s)	281, 508, 677, 2014
MvaI	CCWGG	6 cut(s)	203, 521, 584, 1135, 2058, 2606
MvnI	CGCG	2 cut(s)	1893, 2313
MwoI	GCNNNNNNNGC	6 cut(s)	37, 251, 521, 812, 989, 1724
NciI	CCSGG	1 cut(s)	105
NcoI	CCATGG	2 cut(s)	855, 2250
NmeAIII	GCCGAG	1 cut(s)	1324
NmuCI	GTSAC	7 cut(s)	311, 343, 469, 1228, 1317, 1525, 2140
NruI	TCGCGA	1 cut(s)	2313
NsiI	ATGCAT	2 cut(s)	283, 390
NspI	RCATGY	4 cut(s)	388, 1253, 2171, 2395
NspV	TTCGAA	2 cut(s)	638, 1501
PaeI	GCATGC	1 cut(s)	388
PagI	TCATGA	2 cut(s)	1219, 1534
PaqCI	CACCTGC	1 cut(s)	1718
PasI	CCCWGGG	1 cut(s)	2605
PciSI	GCTCTTC	3 cut(s)	605, 2885, 2992
PctI	GAATGC	4 cut(s)	281, 508, 677, 2014
PflFI	GACNNNGTC	2 cut(s)	13, 317
PflMI	CCANNNNNTGG	2 cut(s)	482, 1688
PkrI	GCNGC	9 cut(s)	7, 339, 400, 805, 808, 1132, 1248, 1717, 2877
PpuMI	RGGWCCY	1 cut(s)	2332
PshBI	ATTAAT	2 cut(s)	2121, 2376
PsiI	TTATAA	2 cut(s)	1962, 2768
Psp1406I	AACGTT	2 cut(s)	2267, 2553
Psp5II	RGGWCCY	1 cut(s)	2332
Psp6I	CCWGG	6 cut(s)	201, 519, 582, 1133, 2056, 2604
PspEI	GGTNACC	1 cut(s)	2140
PspFI	CCCAGC	1 cut(s)	2012
PspGI	CCWGG	6 cut(s)	201, 519, 582, 1133, 2056, 2604
PspOMI	GGGCCC	1 cut(s)	1172
PspPPI	RGGWCCY	1 cut(s)	2332
PstI	CTGCAG	1 cut(s)	811
PstNI	CAGNNNCTG	3 cut(s)	1232, 1410, 1733
PsuI	RGATCY	2 cut(s)	1363, 2842
PsyI	GACNNNGTC	2 cut(s)	13, 317
PvuII	CAGCTG	1 cut(s)	1715
RruI	TCGCGA	1 cut(s)	2313
RsaI	GTAC	8 cut(s)	1048, 1070, 1237, 1466, 1755, 2109, 2407, 2632
RsaNI	GTAC	8 cut(s)	1047, 1069, 1236, 1465, 1754, 2108, 2406, 2631
RseI	CAYNNNNRTG	4 cut(s)	889, 1221, 2249, 2820
SapI	GCTCTTC	3 cut(s)	605, 2885, 2992
SatI	GCNGC	9 cut(s)	6, 338, 399, 804, 807, 1131, 1247, 1716, 2876
ScrFI	CCNGG	7 cut(s)	105, 203, 521, 584, 1135, 2058, 2606
SduI	GDGCHC	3 cut(s)	1176, 1391, 2346
SfaNI	GCATC	9 cut(s)	451, 970, 1186, 1213, 1352, 1427, 1573, 2466, 3106
SfcI	CTRYAG	4 cut(s)	807, 2317, 2352, 2436
SfuI	TTCGAA	2 cut(s)	638, 1501
SinI	GGWCC	7 cut(s)	127, 852, 2090, 2332, 2428, 2445, 3017
SmiMI	CAYNNNNRTG	4 cut(s)	889, 1221, 2249, 2820
SmlI	CTYRAG	2 cut(s)	1597, 2506
SmoI	CTYRAG	2 cut(s)	1597, 2506
SphI	GCATGC	1 cut(s)	388
SsiI	CCGC	4 cut(s)	5, 292, 631, 2519
SspI	AATATT	1 cut(s)	1148
SspMI	CTAG	6 cut(s)	53, 341, 437, 1142, 1577, 2666
StyD4I	CCNGG	7 cut(s)	103, 201, 519, 582, 1133, 2056, 2604
StyI	CCWWGG	6 cut(s)	91, 855, 1438, 1474, 2250, 2575
TaqI	TCGA	7 cut(s)	638, 832, 1501, 1941, 2178, 2598, 3008
TaqII	GACCGA	2 cut(s)	2445, 2627
TatI	WGTACW	4 cut(s)	1464, 1753, 2107, 2405
TauI	GCSGC	1 cut(s)	8
TscAI	CASTG	8 cut(s)	1237, 1357, 1391, 1534, 1984, 2281, 2460, 2566
TseFI	GTSAC	7 cut(s)	311, 343, 469, 1228, 1317, 1525, 2140
TseI	GCWGC	8 cut(s)	337, 398, 803, 806, 1130, 1246, 1715, 2875
Tsp45I	GTSAC	7 cut(s)	311, 343, 469, 1228, 1317, 1525, 2140
TspGWI	ACGGA	8 cut(s)	307, 992, 1307, 1666, 1980, 2018, 2226, 2953
TspRI	CASTG	8 cut(s)	1237, 1357, 1391, 1534, 1984, 2281, 2460, 2566
Tth111I	GACNNNGTC	2 cut(s)	13, 317
Van91I	CCANNNNNTGG	2 cut(s)	482, 1688
Vha464I	CTTAAG	1 cut(s)	1597
VneI	GTGCAC	1 cut(s)	2342
VpaK11BI	GGWCC	7 cut(s)	127, 852, 2090, 2332, 2428, 2445, 3017
VspI	ATTAAT	2 cut(s)	2121, 2376
XapI	RAATTY	9 cut(s)	269, 461, 620, 1429, 1503, 2148, 2411, 2582, 2701
XceI	RCATGY	4 cut(s)	388, 1253, 2171, 2395
XcmI	CCANNNNNNNNNTGG	3 cut(s)	146, 483, 2354
XmiI	GTMKAC	1 cut(s)	1081
XspI	CTAG	6 cut(s)	53, 341, 437, 1142, 1577, 2666
Zsp2I	ATGCAT	2 cut(s)	283, 390

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.