Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0001301

Physical Location & Seq

Genomic Coordinates Forward (+)

7732094 .. 7738986

Gene Structure 6893 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G008490.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3066 bp

ATGGCGGTTGAAGCTTGTTGCTTACGGCTTCTCGTGGTACTAATTGTGTTTCTAAATGTAGAATCCAAGTTCATGGCCTACAATACAACGTCGCAGATTGTCCCCGGAAAGCTTAACGTTCATTTAGTTCCTCACACCCATGACGATGTCGGATGGCGGAAAACCGTTGACCAGTACTACACCGGTGCCAATAATTCTCTCCTGGACGCTCCCGCTTGTGTTCGAAACGTGTTGGATTCTTTAGTTCCCGCATTGTTGGCCGATAAGAACCGCAAGTTCATATATGCTGAGCAGGCATTTTTTCAACTATGGTGGAGAGAACAGAGCGAGGCGATCCAAGACACAGTCAAGAAGCTAGTCGGCTCTGGCCAACTGGAATTCATAAATGGGGGGATGTGCATGCAAGATGAGGCTGCTACGCATTACATTGATATGATTGATCAGACGACTCTAGGGCATCGATTTATTAAGCAAGAGTTCAATGTGACTCCAAGAGTTGGCTGGCAAATCGATTCGTTTGGACATTCTTCTGTGCAGGCTTACCTTTTGGCGGCTGAGGTTGGCTTTGATTCCCTATTTTTTGGACGAATCGACTACCAAGATATAGCTACGAGGAAAAATGAGAAAAGCCTTGAGGTTGTGTGGCGGGGTTCTAAGAGTCTCGGTTCATCTGCACAGATTTTTGCTGGTGCATTCCCTACGCTATATGTTCCTCCTTCTCATAATTTCTACTTTGACACTGATGACGTCCCCCCTCTTGTACAAGATGATATGAATCTTGGTGACTACAATGTGGCAGATCGCATTAATGATTTTGTATCTGAAGCAATATCACAAGCAAACATTACTCGTACAAATCATATCATGTGGACCATGGGAAAGGATTTCGCGTATCAATATGCTAATTCATGGTTCAGTCAGATGGACAAATTCATTCATCATGTGAATCAAGATGGGCGTGTCAATGCCCTATACTCAACCCCATCAATATACACCGATGCCAAACATGCAACAAATGAGTCCTGGCCAATCAAGAGTGATGACTTCTTTCCATATGCTAGTGAAGTGAATGCATACTGGACTGGATATTTTACAAGCAGGCCAGCCCTCAAACGTTATGTTAGAATGATGAGTAGCTACTATTTGGCAGCAAGGCAATTGGAATTTTTTACGGGAATAAGTAAATCAGGGCCTAACACAGATTCTCTGGCTGATGCTTTAGCAATTGCTCAACATCATGATGCAGTTAGCGGCACATCTAGGCAGCACGTAGCTGATGATTATACAAAACGACTTTCAATAGGTTACAAGGAGGCTGAGAAGGTTGTTGCAGAATCACTTTCTTGCTTGATAAGTTCAAGATTAAGCACTGGTTGCAAGAGTGTAGTAACAAAGTTTCAACAGTGTCCACTTCTGAATATTAGCTATTGCCCCCCGTCAGAAGTTGATCTTTCAAAGGGAAAACACCTGGTTATTGTTGTCTATAACCCTCTGGGATGGAAAAGAGAGGATGTGATAAAGATTCCTGTTGTCAGTGAAAAAGTCACTGTAAAGGACTCCACCGGAAAGGAAATTGAATCACAGATTCTACCGCTGCTTAATGCATCTGTCAGCATAAGAAATGACCATGTTAAGGCATATTTGGGTATAGCACCGAGTGTGACCACAAGTTATTGGCTTGCATTTTCAGCAACTGTACCACCTCTTGGTTTTAGCACTTACATGGTCTCAAGTGCTCTCACACAGACAGCTACTACTTCAGAGAGACATACAGTGTACAAGACAGAAACAAGTCAAAACGAAACAATAAAAGTTGGGCCTGGAAACTTGAAACTCATTTATTCTGGAAACGATGGAAAACTGATTCAATACACAAACAGCAGAAGCTCGGTCAAAGAGTTGGTAACGCAATCATTTAGCTACTACCCTGGAGATGATGGAAGTGTGAAGGTTTTAGGTGAGGATGGAAATCTGCATTCTCAGGCCTCAGGAGCATATGTCTTCCGTCCTAATGGTACATATCCCATTAAATCCGAAGGGCAGGTTTCTTTGACTATTTTAAGGGGACCATTGTTGGATGAAATACATGAAAGGATCAACTCTTGGATATATCAGGTCACTAGAGTGTACAGGGAAAAAGAGCATGCTGAGATTGAATTTACTATAGGGCCTATACCAGTTGATGATAGAATAGGCAAAGAGATTGTAACTAAGATTACAACCAGCATGAGAACCAACAAACAATTCTACACAGATTCTAACGGGCGCGATTTTATTCAAAGAATTCGAGACTACAGAAAAGACTGGGACCTGGAAGTAAACCAACCTGTCGCAGGAAACTATTATCCGATTAATCTGGGAATCTATACTAAAGATAACAACACAGAGCTGTCAGTATTAGTGGATAGATCTGTAGGGGGATCCAGCATTGTGGACGGGCAATTGGAACTGATGCTTCATAGGAGATTGCTTGCAGATGATGACAAAGGTATTGCAGAACAACTAGATGAAATGGTGTGCATTGAACATGATTGCAAAGGACTAACAATTACAGGAAAGTACCACCTGAGAATTGATCCTTTAGGAGAGGGTGCTAAGTGGCGTCGATCATTTGGTCAGGAGATATATTCTCCACTTCTATTAGCCTTCACAGAACAAAATGGAGATGACTGGACAAACTCTCATGTCACAACCTTTTCGGGAATGGATCCTTCCTACACTTTACCCGATAATGTTGCAATTATAACTCTCCAGGAGCTGGAAGATGGGAAAGTTCTCTTTCGCCTGGCGCATTTATACGAGATTGGAGAGGACAAGCATCTTTCAGTTATGGCAAGTGTGGAACTGAAAAAGGTGTTTGCAAACAAGAAGATAAAGCATATAACAGAAATGAGCTTATCTGCTAACCAAGAAAGAAGGGAAATGGAAAGGAAGAGACTGGTTTGGAATGTGCAAGGCTCTTCTGAGGAAGGACGGAAAGGCTTGAGGGGAGGACCTGTTGATCCAACAAAGCTAGTGGTGAATCTTGCTCCAATGGAAATTCGAACATTTACCATCGACTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Alpha-mann_mid Glyco_hydro_38 Glyco_hydro_38C

Protein Analysis

1021

Amino Acids

114.9

Weight (kDa)

5.92

Isoelectric Point (pI)

34.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	39 - 353	2.2e-78	Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid	PF09261	358 - 450	2.4e-22	Alpha mannosidase middle domain
Laman-like_dom	PF21260	553 - 576	6.6e-09	Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C	PF07748	607 - 827	5.5e-42	Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2	PF17677	911 - 1017	2.7e-07	Glycosyl hydrolases family 38 C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001301)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13980 AT5G13980 AT5G13980
fragaria_vesca	FvH4_5g09420 FvH4_5g09421 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272
malus_domestica	MD06G1131600.v1.1 MD09G1002900.v1.1 MD17G1007000.v1.1
prunus_persica	Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.5G142600_v2.0.a1 Prupe.5G142600_v2.0.a1
pyrus_communis	pycom06g12300 pycom06g12310 pycom111g00240 pycom17g00260
rosa_chinensis	RchiOBHm_Chr2g0176471 RchiOBHm_Chr2g0176481 RchiOBHm_Chr7g0188771 RchiOBHm_Chr7g0188781
rosa_laevigata	RLG00000004661 RLG00000004662 RLG00000022416
rosa_multiflora	Rmu_sc0000303.1_g000057 Rmu_sc0003642.1_g000008 Rmu_sc0011904.1_g000012
rosa_roxburghii	Rroxscaffold_2G00076370 Rroxscaffold_3G00266040 Rroxscaffold_3G00266050
rosa_rugosa	Rorug02G0595000 Rorug06G0493700
rosa_samantha	Rh2AG673300 Rh2BG684600 Rh2CG647400 Rh2DG698200 Rh7AG098300 Rh7AG098400 Rh7BG099900 Rh7BG100200 Rh7CG101600 Rh7CG101700 Rh7DG100100 Rh7DG100200
rosa_wichuraiana	Rw0G010390 Rw2G055220 Rw7G008480 Rw7G008490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2744
AatII	GACGTC	1 cut(s)	750
Acc36I	ACCTGC	1 cut(s)	2032
AccB1I	GGYRCC	1 cut(s)	185
AccB7I	CCANNNNNTGG	3 cut(s)	497, 1706, 2758
AccII	CGCG	2 cut(s)	890, 2268
AciI	CCGC	9 cut(s)	5, 157, 213, 249, 271, 551, 646, 1251, 1592
AclI	AACGTT	2 cut(s)	117, 1114
AclWI	GGATC	8 cut(s)	328, 2102, 2415, 2428, 2570, 2702, 2715, 2996
AcoI	YGGCCR	3 cut(s)	258, 367, 1025
AcsI	RAATTY	6 cut(s)	377, 929, 1163, 2156, 2283, 3039
AcuI	CTGAAG	2 cut(s)	843, 1743
AcyI	GRCGYC	2 cut(s)	747, 2602
AdeI	CACNNNGTG	1 cut(s)	1658
AfaI	GTAC	9 cut(s)	39, 176, 762, 853, 1698, 1778, 2017, 2129, 2561
AfiI	CCNNNNNNNGG	6 cut(s)	497, 550, 1633, 1706, 2333, 2758
AflIII	ACRYGT	1 cut(s)	228
AgeI	ACCGGT	1 cut(s)	182
AjnI	CCWGG	8 cut(s)	201, 1022, 1467, 1819, 1927, 2310, 2751, 2784
AjuI	GAANNNNNNNTTGG	2 cut(s)	2228, 2260
AleI	CACNNNNGTG	1 cut(s)	2123
Alw21I	GWGCWC	1 cut(s)	1738
Alw26I	GTCTC	5 cut(s)	665, 1732, 1759, 2283, 2929
AlwI	GGATC	8 cut(s)	328, 2102, 2415, 2428, 2570, 2702, 2715, 2996
AlwNI	CAGNNNCTG	2 cut(s)	1694, 2758
AoxI	GGCC	9 cut(s)	75, 258, 367, 1025, 1100, 1190, 1817, 1983, 2168
ApeKI	GCWGC	4 cut(s)	413, 1148, 1264, 1594
ApoI	RAATTY	6 cut(s)	377, 929, 1163, 2156, 2283, 3039
AseI	ATTAAT	2 cut(s)	807, 2352
AsiGI	ACCGGT	1 cut(s)	182
AspLEI	GCGC	2 cut(s)	2268, 2791
AspS9I	GGNCC	7 cut(s)	870, 1190, 1817, 2066, 2168, 2308, 2993
AsuC2I	CCSGG	1 cut(s)	105
AsuHPI	GGTGA	3 cut(s)	794, 1970, 3031
AsuII	TTCGAA	2 cut(s)	223, 3043
AvaII	GGWCC	4 cut(s)	870, 2066, 2308, 2993
AxyI	CCTNAGG	1 cut(s)	1987
BalI	TGGCCA	2 cut(s)	369, 1027
BamHI	GGATCC	2 cut(s)	2420, 2707
BanI	GGYRCC	1 cut(s)	185
BauI	CACGAG	1 cut(s)	32
BbsI	GAAGAC	1 cut(s)	1993
Bbv12I	GWGCWC	1 cut(s)	1738
BbvCI	CCTCAGC	1 cut(s)	555
BbvI	GCAGC	4 cut(s)	400, 1160, 1276, 1581
BceAI	ACGGC	1 cut(s)	41
BcgI	CGANNNNNNTGC	2 cut(s)	2717, 2751
BciT130I	CCWGG	8 cut(s)	203, 1024, 1469, 1821, 1929, 2312, 2753, 2786
BclI	TGATCA	1 cut(s)	439
BcnI	CCSGG	1 cut(s)	105
BcoDI	GTCTC	5 cut(s)	665, 1732, 1759, 2283, 2929
BfaI	CTAG	7 cut(s)	356, 452, 1059, 1260, 2121, 2504, 3014
BfmI	CTRYAG	3 cut(s)	2163, 2293, 2412
BfuAI	ACCTGC	1 cut(s)	2032
BglII	AGATCT	1 cut(s)	2408
BisI	GCNGC	6 cut(s)	414, 552, 1149, 1252, 1265, 1595
BlpI	GCTNAGC	1 cut(s)	288
BlsI	GCNGC	6 cut(s)	415, 553, 1150, 1253, 1266, 1596
BmcAI	AGTACT	1 cut(s)	176
Bme1390I	CCNGG	9 cut(s)	105, 203, 1024, 1469, 1821, 1929, 2312, 2753, 2786
Bme18I	GGWCC	4 cut(s)	870, 2066, 2308, 2993
BmgT120I	GGNCC	7 cut(s)	870, 1190, 1817, 2066, 2168, 2308, 2993
BmiI	GGNNCC	5 cut(s)	187, 2067, 2309, 2422, 2709
BmrFI	CCNGG	9 cut(s)	105, 203, 1024, 1469, 1821, 1929, 2312, 2753, 2786
BmrI	ACTGGG	1 cut(s)	2314
BmsI	GCATC	7 cut(s)	466, 988, 1204, 1231, 1613, 2442, 2827
BmuI	ACTGGG	1 cut(s)	2314
BpiI	GAAGAC	1 cut(s)	1993
BpmI	CTGGAG	2 cut(s)	1950, 2735
Bpu10I	CCTNAGC	1 cut(s)	555
Bpu1102I	GCTNAGC	1 cut(s)	288
Bpu14I	TTCGAA	2 cut(s)	223, 3043
BpuEI	CTTGAG	3 cut(s)	653, 1714, 3004
BpuMI	CCSGG	1 cut(s)	105
Bsa29I	ATCGAT	2 cut(s)	460, 510
BsaAI	YACGTR	1 cut(s)	1270
BsaBI	GATNNNNATC	2 cut(s)	774, 1968
BsaHI	GRCGYC	2 cut(s)	747, 2602
BsaI	GGTCTC	1 cut(s)	1732
BsaJI	CCNNGG	3 cut(s)	103, 873, 1927
BsaWI	WCCGGW	2 cut(s)	182, 1562
BsaXI	ACNNNNNCTCC	4 cut(s)	1983, 2013, 2982, 3012
Bsc4I	CCNNNNNNNGG	6 cut(s)	497, 550, 1633, 1706, 2333, 2758
Bse118I	RCCGGY	1 cut(s)	182
Bse1I	ACTGG	9 cut(s)	172, 378, 1082, 1087, 1375, 2177, 2309, 2675, 2943
Bse21I	CCTNAGG	1 cut(s)	1987
Bse8I	GATNNNNATC	2 cut(s)	774, 1968
BseBI	CCWGG	8 cut(s)	203, 1024, 1469, 1821, 1929, 2312, 2753, 2786
BseCI	ATCGAT	2 cut(s)	460, 510
BseDI	CCNNGG	3 cut(s)	103, 873, 1927
BseGI	GGATG	6 cut(s)	158, 399, 1502, 1516, 1969, 2083
BseJI	GATNNNNATC	2 cut(s)	774, 1968
BseLI	CCNNNNNNNGG	6 cut(s)	497, 550, 1633, 1706, 2333, 2758
BseMII	CTCAG	8 cut(s)	279, 546, 1308, 1994, 2001, 2139, 2558, 2955
BseNI	ACTGG	9 cut(s)	172, 378, 1082, 1087, 1375, 2177, 2309, 2675, 2943
BseXI	GCAGC	4 cut(s)	400, 1160, 1276, 1581
BsgI	GTGCAG	2 cut(s)	554, 657
Bsh1236I	CGCG	2 cut(s)	890, 2268
BshFI	GGCC	9 cut(s)	77, 260, 369, 1027, 1102, 1192, 1819, 1985, 2170
BshNI	GGYRCC	1 cut(s)	185
BshTI	ACCGGT	1 cut(s)	182
BshVI	ATCGAT	2 cut(s)	460, 510
BsiHKAI	GWGCWC	1 cut(s)	1738
BsiSI	CCGG	3 cut(s)	105, 183, 1563
BslFI	GGGAC	4 cut(s)	86, 734, 2079, 2321
BslI	CCNNNNNNNGG	6 cut(s)	497, 550, 1633, 1706, 2333, 2758
BsmAI	GTCTC	5 cut(s)	665, 1732, 1759, 2283, 2929
BsmFI	GGGAC	4 cut(s)	86, 734, 2079, 2321
BsmI	GAATGC	3 cut(s)	692, 1075, 1975
BsnI	GGCC	9 cut(s)	77, 260, 369, 1027, 1102, 1192, 1819, 1985, 2170
Bso31I	GGTCTC	1 cut(s)	1732
Bsp119I	TTCGAA	2 cut(s)	223, 3043
Bsp1286I	GDGCHC	1 cut(s)	1738
Bsp1407I	TGTACA	3 cut(s)	760, 1776, 2127
Bsp1720I	GCTNAGC	1 cut(s)	288
Bsp19I	CCATGG	1 cut(s)	873
BspACI	CCGC	9 cut(s)	5, 157, 213, 249, 271, 551, 646, 1251, 1592
BspANI	GGCC	9 cut(s)	77, 260, 369, 1027, 1102, 1192, 1819, 1985, 2170
BspCNI	CTCAG	8 cut(s)	280, 547, 1309, 1993, 2000, 2140, 2559, 2956
BspDI	ATCGAT	2 cut(s)	460, 510
BspFNI	CGCG	2 cut(s)	890, 2268
BspHI	TCATGA	1 cut(s)	1237
BspLI	GGNNCC	5 cut(s)	187, 2067, 2309, 2422, 2709
BspMI	ACCTGC	1 cut(s)	2032
BspPI	GGATC	8 cut(s)	328, 2102, 2415, 2428, 2570, 2702, 2715, 2996
BspQI	GCTCTTC	1 cut(s)	2965
BspT104I	TTCGAA	2 cut(s)	223, 3043
BspT107I	GGYRCC	1 cut(s)	185
BspTNI	GGTCTC	1 cut(s)	1732
BsrFI	RCCGGY	1 cut(s)	182
BsrGI	TGTACA	3 cut(s)	760, 1776, 2127
BsrI	ACTGG	9 cut(s)	172, 378, 1082, 1087, 1375, 2177, 2309, 2675, 2943
BssAI	RCCGGY	1 cut(s)	182
BssECI	CCNNGG	3 cut(s)	103, 873, 1927
BssNI	GRCGYC	2 cut(s)	747, 2602
BssSI	CACGAG	1 cut(s)	32
BssT1I	CCWWGG	1 cut(s)	873
Bst2BI	CACGAG	1 cut(s)	32
Bst2UI	CCWGG	8 cut(s)	203, 1024, 1469, 1821, 1929, 2312, 2753, 2786
Bst4CI	ACNGT	6 cut(s)	166, 346, 1404, 1549, 1696, 1774
Bst6I	CTCTTC	2 cut(s)	2927, 2965
BstACI	GRCGYC	2 cut(s)	747, 2602
BstAPI	GCANNNNNTGC	1 cut(s)	1374
BstAUI	TGTACA	3 cut(s)	760, 1776, 2127
BstBAI	YACGTR	1 cut(s)	1270
BstBI	TTCGAA	2 cut(s)	223, 3043
BstC8I	GCNNGC	9 cut(s)	294, 401, 503, 537, 1100, 1104, 1680, 2145, 2472
BstDSI	CCRYGG	1 cut(s)	873
BstENI	CCTNNNNNAGG	1 cut(s)	2331
BstF5I	GGATG	6 cut(s)	158, 399, 1502, 1516, 1969, 2083
BstFNI	CGCG	2 cut(s)	890, 2268
BstHHI	GCGC	2 cut(s)	2268, 2791
BstMAI	GTCTC	5 cut(s)	665, 1732, 1759, 2283, 2929
BstMWI	GCNNNNNNNGC	7 cut(s)	11, 257, 293, 1007, 1374, 1688, 1991
BstNI	CCWGG	8 cut(s)	203, 1024, 1469, 1821, 1929, 2312, 2753, 2786
BstNSI	RCATGY	3 cut(s)	403, 1010, 2147
BstSCI	CCNGG	9 cut(s)	103, 201, 1022, 1467, 1819, 1927, 2310, 2751, 2784
BstSFI	CTRYAG	3 cut(s)	2163, 2293, 2412
BstUI	CGCG	2 cut(s)	890, 2268
BstV1I	GCAGC	4 cut(s)	400, 1160, 1276, 1581
BstV2I	GAAGAC	1 cut(s)	1993
BstX2I	RGATCY	3 cut(s)	2408, 2420, 2707
BstXI	CCANNNNNNTGG	2 cut(s)	73, 2431
BstYI	RGATCY	3 cut(s)	2408, 2420, 2707
Bsu15I	ATCGAT	2 cut(s)	460, 510
Bsu36I	CCTNAGG	1 cut(s)	1987
BsuRI	GGCC	9 cut(s)	77, 260, 369, 1027, 1102, 1192, 1819, 1985, 2170
BsuTUI	ATCGAT	2 cut(s)	460, 510
BtgI	CCRYGG	1 cut(s)	873
BtsCI	GGATG	6 cut(s)	158, 399, 1502, 1516, 1969, 2083
BtsIMutI	CAGTG	6 cut(s)	738, 1368, 1409, 1540, 1545, 1779
BveI	ACCTGC	1 cut(s)	2032
Cac8I	GCNNGC	9 cut(s)	294, 401, 503, 537, 1100, 1104, 1680, 2145, 2472
CaiI	CAGNNNCTG	2 cut(s)	1694, 2758
CciI	TCATGA	1 cut(s)	1237
CfoI	GCGC	2 cut(s)	2268, 2791
Cfr10I	RCCGGY	1 cut(s)	182
Cfr13I	GGNCC	7 cut(s)	870, 1190, 1817, 2066, 2168, 2308, 2993
ClaI	ATCGAT	2 cut(s)	460, 510
CseI	GACGC	2 cut(s)	215, 2591
CsiI	ACCWGGT	1 cut(s)	1467
Csp6I	GTAC	9 cut(s)	38, 175, 761, 852, 1697, 1777, 2016, 2128, 2560
CspAI	ACCGGT	1 cut(s)	182
CspCI	CAANNNNNGTGG	4 cut(s)	293, 328, 2997, 3032
CviQI	GTAC	9 cut(s)	38, 175, 761, 852, 1697, 1777, 2016, 2128, 2560
DraIII	CACNNNGTG	1 cut(s)	1658
EaeI	YGGCCR	3 cut(s)	258, 367, 1025
Eam1104I	CTCTTC	2 cut(s)	2927, 2965
EarI	CTCTTC	2 cut(s)	2927, 2965
EciI	GGCGGA	1 cut(s)	172
Eco130I	CCWWGG	1 cut(s)	873
Eco147I	AGGCCT	1 cut(s)	1985
Eco31I	GGTCTC	1 cut(s)	1732
Eco47I	GGWCC	4 cut(s)	870, 2066, 2308, 2993
Eco57I	CTGAAG	2 cut(s)	843, 1743
Eco81I	CCTNAGG	1 cut(s)	1987
EcoNI	CCTNNNNNAGG	1 cut(s)	2331
EcoO109I	RGGNCCY	4 cut(s)	1190, 2168, 2308, 2993
EcoRI	GAATTC	2 cut(s)	377, 2283
EcoRII	CCWGG	8 cut(s)	201, 1022, 1467, 1819, 1927, 2310, 2751, 2784
EcoT14I	CCWWGG	1 cut(s)	873
EcoT22I	ATGCAT	2 cut(s)	1075, 1606
ErhI	CCWWGG	1 cut(s)	873
FalI	AAGNNNNNCTT	2 cut(s)	1434, 1466
FaqI	GGGAC	4 cut(s)	86, 734, 2079, 2321
FauI	CCCGC	3 cut(s)	220, 256, 639
FauNDI	CATATG	2 cut(s)	1054, 1996
FbaI	TGATCA	1 cut(s)	439
Fnu4HI	GCNGC	6 cut(s)	414, 552, 1149, 1252, 1265, 1595
FokI	GGATG	6 cut(s)	165, 406, 1509, 1523, 1976, 2090
Fsp4HI	GCNGC	6 cut(s)	414, 552, 1149, 1252, 1265, 1595
FspBI	CTAG	7 cut(s)	356, 452, 1059, 1260, 2121, 2504, 3014
GlaI	GCGC	2 cut(s)	2267, 2790
GluI	GCNGC	6 cut(s)	414, 552, 1149, 1252, 1265, 1595
GsuI	CTGGAG	2 cut(s)	1950, 2735
HaeIII	GGCC	9 cut(s)	77, 260, 369, 1027, 1102, 1192, 1819, 1985, 2170
HapII	CCGG	3 cut(s)	105, 183, 1563
HgaI	GACGC	2 cut(s)	215, 2591
HhaI	GCGC	2 cut(s)	2268, 2791
Hin1I	GRCGYC	2 cut(s)	747, 2602
Hin6I	GCGC	2 cut(s)	2266, 2789
HinP1I	GCGC	2 cut(s)	2266, 2789
HincII	GTYRAC	1 cut(s)	169
HindII	GTYRAC	1 cut(s)	169
HindIII	AAGCTT	2 cut(s)	12, 110
HpaII	CCGG	3 cut(s)	105, 183, 1563
HphI	GGTGA	3 cut(s)	794, 1970, 3031
Hpy166II	GTNNAC	7 cut(s)	169, 870, 1409, 1777, 2128, 2320, 2434
Hpy8I	GTNNAC	7 cut(s)	169, 870, 1409, 1777, 2128, 2320, 2434
Hpy99I	CGWCG	2 cut(s)	94, 2607
HpyAV	CCTTC	8 cut(s)	726, 1315, 1942, 2030, 2656, 2721, 2910, 2963
HpyCH4III	ACNGT	6 cut(s)	166, 346, 1404, 1549, 1696, 1774
HpyCH4IV	ACGT	6 cut(s)	89, 117, 228, 747, 1114, 1269
HpyF10VI	GCNNNNNNNGC	7 cut(s)	11, 257, 293, 1007, 1374, 1688, 1991
HpySE526I	ACGT	6 cut(s)	89, 117, 228, 747, 1114, 1269
Hsp92I	GRCGYC	2 cut(s)	747, 2602
HspAI	GCGC	2 cut(s)	2266, 2789
Ksp22I	TGATCA	1 cut(s)	439
LguI	GCTCTTC	1 cut(s)	2965
LmnI	GCTCC	4 cut(s)	214, 1991, 2755, 3034
Lsp1109I	GCAGC	4 cut(s)	400, 1160, 1276, 1581
LweI	GCATC	7 cut(s)	466, 988, 1204, 1231, 1613, 2442, 2827
MabI	ACCWGGT	1 cut(s)	1467
MaeI	CTAG	7 cut(s)	356, 452, 1059, 1260, 2121, 2504, 3014
MaeII	ACGT	6 cut(s)	89, 117, 228, 747, 1114, 1269
MboII	GAAGA	6 cut(s)	519, 1993, 2774, 2881, 2944, 2952
MfeI	CAATTG	3 cut(s)	1157, 1224, 2441
MflI	RGATCY	3 cut(s)	2408, 2420, 2707
MhlI	GDGCHC	1 cut(s)	1738
MlsI	TGGCCA	2 cut(s)	369, 1027
MluNI	TGGCCA	2 cut(s)	369, 1027
MlyI	GAGTC	5 cut(s)	442, 481, 667, 1028, 1550
MmeI	TCCRAC	4 cut(s)	130, 213, 2055, 3029
Mox20I	TGGCCA	2 cut(s)	369, 1027
Mph1103I	ATGCAT	2 cut(s)	1075, 1606
MscI	TGGCCA	2 cut(s)	369, 1027
MseI	TTAA	9 cut(s)	114, 468, 807, 1364, 1599, 1632, 2028, 2060, 2352
MslI	CAYNNNNRTG	6 cut(s)	138, 144, 431, 1239, 1721, 2123
Msp20I	TGGCCA	2 cut(s)	369, 1027
MspA1I	CMGCKG	1 cut(s)	1594
MspI	CCGG	3 cut(s)	105, 183, 1563
MspR9I	CCNGG	9 cut(s)	105, 203, 1024, 1469, 1821, 1929, 2312, 2753, 2786
MunI	CAATTG	3 cut(s)	1157, 1224, 2441
Mva1269I	GAATGC	3 cut(s)	692, 1075, 1975
MvaI	CCWGG	8 cut(s)	203, 1024, 1469, 1821, 1929, 2312, 2753, 2786
MvnI	CGCG	2 cut(s)	890, 2268
MwoI	GCNNNNNNNGC	7 cut(s)	11, 257, 293, 1007, 1374, 1688, 1991
NciI	CCSGG	1 cut(s)	105
NcoI	CCATGG	1 cut(s)	873
NdeI	CATATG	2 cut(s)	1054, 1996
NlaIV	GGNNCC	5 cut(s)	187, 2067, 2309, 2422, 2709
NmuCI	GTSAC	6 cut(s)	484, 782, 1543, 1660, 2116, 2686
NsiI	ATGCAT	2 cut(s)	1075, 1606
NspI	RCATGY	3 cut(s)	403, 1010, 2147
NspV	TTCGAA	2 cut(s)	223, 3043
OliI	CACNNNNGTG	1 cut(s)	2123
PaeI	GCATGC	2 cut(s)	403, 2147
PagI	TCATGA	1 cut(s)	1237
PceI	AGGCCT	1 cut(s)	1985
PciSI	GCTCTTC	1 cut(s)	2965
PctI	GAATGC	3 cut(s)	692, 1075, 1975
PflFI	GACNNNGTC	2 cut(s)	146, 344
PflMI	CCANNNNNTGG	3 cut(s)	497, 1706, 2758
PfoI	TCCNGGA	2 cut(s)	201, 2751
PinAI	ACCGGT	1 cut(s)	182
PkrI	GCNGC	6 cut(s)	415, 553, 1150, 1253, 1266, 1596
PleI	GAGTC	5 cut(s)	442, 481, 666, 1027, 1550
PpsI	GAGTC	5 cut(s)	442, 481, 666, 1027, 1550
Ppu21I	YACGTR	1 cut(s)	1270
PpuMI	RGGWCCY	2 cut(s)	2308, 2993
PshBI	ATTAAT	2 cut(s)	807, 2352
PsiI	TTATAA	1 cut(s)	2744
Psp1406I	AACGTT	2 cut(s)	117, 1114
Psp5II	RGGWCCY	2 cut(s)	2308, 2993
Psp6I	CCWGG	8 cut(s)	201, 1022, 1467, 1819, 1927, 2310, 2751, 2784
PspGI	CCWGG	8 cut(s)	201, 1022, 1467, 1819, 1927, 2310, 2751, 2784
PspN4I	GGNNCC	5 cut(s)	187, 2067, 2309, 2422, 2709
PspPI	GGNCC	7 cut(s)	870, 1190, 1817, 2066, 2168, 2308, 2993
PspPPI	RGGWCCY	2 cut(s)	2308, 2993
PstNI	CAGNNNCTG	2 cut(s)	1694, 2758
PsuI	RGATCY	3 cut(s)	2408, 2420, 2707
PsyI	GACNNNGTC	2 cut(s)	146, 344
RsaI	GTAC	9 cut(s)	39, 176, 762, 853, 1698, 1778, 2017, 2129, 2561
RsaNI	GTAC	9 cut(s)	38, 175, 761, 852, 1697, 1777, 2016, 2128, 2560
RseI	CAYNNNNRTG	6 cut(s)	138, 144, 431, 1239, 1721, 2123
SapI	GCTCTTC	1 cut(s)	2965
SaqAI	TTAA	9 cut(s)	114, 468, 807, 1364, 1599, 1632, 2028, 2060, 2352
SatI	GCNGC	6 cut(s)	414, 552, 1149, 1252, 1265, 1595
Sau96I	GGNCC	7 cut(s)	870, 1190, 1817, 2066, 2168, 2308, 2993
ScaI	AGTACT	1 cut(s)	176
SchI	GAGTC	5 cut(s)	442, 481, 667, 1028, 1550
ScrFI	CCNGG	9 cut(s)	105, 203, 1024, 1469, 1821, 1929, 2312, 2753, 2786
SduI	GDGCHC	1 cut(s)	1738
SexAI	ACCWGGT	1 cut(s)	1467
SfaNI	GCATC	7 cut(s)	466, 988, 1204, 1231, 1613, 2442, 2827
SfcI	CTRYAG	3 cut(s)	2163, 2293, 2412
SfuI	TTCGAA	2 cut(s)	223, 3043
SgrAI	CRCCGGYG	1 cut(s)	182
SinI	GGWCC	4 cut(s)	870, 2066, 2308, 2993
SmiMI	CAYNNNNRTG	6 cut(s)	138, 144, 431, 1239, 1721, 2123
SmlI	CTYRAG	3 cut(s)	632, 1729, 2983
SmoI	CTYRAG	3 cut(s)	632, 1729, 2983
SphI	GCATGC	2 cut(s)	403, 2147
SseBI	AGGCCT	1 cut(s)	1985
SsiI	CCGC	9 cut(s)	5, 157, 213, 249, 271, 551, 646, 1251, 1592
SspI	AATATT	1 cut(s)	1420
SspMI	CTAG	7 cut(s)	356, 452, 1059, 1260, 2121, 2504, 3014
StuI	AGGCCT	1 cut(s)	1985
StyD4I	CCNGG	9 cut(s)	103, 201, 1022, 1467, 1819, 1927, 2310, 2751, 2784
StyI	CCWWGG	1 cut(s)	873
TaaI	ACNGT	6 cut(s)	166, 346, 1404, 1549, 1696, 1774
TaiI	ACGT	6 cut(s)	92, 120, 231, 750, 1117, 1272
TaqI	TCGA	8 cut(s)	223, 460, 510, 591, 2287, 2605, 3043, 3057
TaqII	GACCGA	1 cut(s)	1879
TatI	WGTACW	4 cut(s)	174, 760, 1776, 2127
TauI	GCSGC	2 cut(s)	554, 1254
Tru1I	TTAA	9 cut(s)	114, 468, 807, 1364, 1599, 1632, 2028, 2060, 2352
Tru9I	TTAA	9 cut(s)	114, 468, 807, 1364, 1599, 1632, 2028, 2060, 2352
TscAI	CASTG	6 cut(s)	745, 1375, 1409, 1540, 1552, 1779
TseFI	GTSAC	6 cut(s)	484, 782, 1543, 1660, 2116, 2686
TseI	GCWGC	4 cut(s)	413, 1148, 1264, 1594
Tsp45I	GTSAC	6 cut(s)	484, 782, 1543, 1660, 2116, 2686
TspGWI	ACGGA	2 cut(s)	1994, 2989
TspRI	CASTG	6 cut(s)	745, 1375, 1409, 1540, 1552, 1779
Tth111I	GACNNNGTC	2 cut(s)	146, 344
Van91I	CCANNNNNTGG	3 cut(s)	497, 1706, 2758
VpaK11BI	GGWCC	4 cut(s)	870, 2066, 2308, 2993
VspI	ATTAAT	2 cut(s)	807, 2352
XagI	CCTNNNNNAGG	1 cut(s)	2331
XapI	RAATTY	6 cut(s)	377, 929, 1163, 2156, 2283, 3039
XceI	RCATGY	3 cut(s)	403, 1010, 2147
XcmI	CCANNNNNNNNNTGG	1 cut(s)	498
XspI	CTAG	7 cut(s)	356, 452, 1059, 1260, 2121, 2504, 3014
ZraI	GACGTC	1 cut(s)	748
ZrmI	AGTACT	1 cut(s)	176
Zsp2I	ATGCAT	2 cut(s)	1075, 1606

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.