Prupe.5G142600_v2.0.a1

Lysosomal alpha-mannosidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0001301
Physical Location & Seq
Forward (+)
13207624 .. 13214941
7318 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G142600.1

Sequence Viewer

Length: 3045 bp
ATGGCGCTTGAAGCTTGCTTTCGGCTGCTCTTGCTACTGCTTCTGTTTCTAATTGCAGAACCCAAGTACGTGCAGTACAATACGACGTCGCGCCTCGTCCCCGGGAAGCTCAACGTTCATTTAGTCCCTCACACCCACGACGACGTCGGATGGTTGAAGACCGTTGACCAGTACTATGTTGGCTCTAACAATTCCATTCAGGGAGCTTGTGTTCAAAACGTGCTGGATTCTTTGGTTCCCGCATTGCTGGCCGATAAGAACCGCAAGTTCATATATGTTGAGCAGGCGTTTTTTCAACGTTGGTGGAGAGAGCAGAGTGAGGGAACCCAGAACACGGTCAGGCGGCTAGTCAACAATGGTCAACTGGAACTCATAAATGGGGGGATGTGCATGCATGATGAGGCTGCTCCTCATTACATCGATATGATCGATCAGACGACCCTCGGGCATCAATTTATTAAAGAAGAATTCAATATGACTCCAAGAATTGGCTGGCAAATCGACCCATTTGGACATTCTGCAGTGCAGGCTTACCTTTTGGGGGCCGAGGCTGGATTTGACTCTCTTTTCTTTGCGCGAATCGACTACCAAGACAGAGAGAAACGGAAAAATGAGAAAAGCCTTGAGGTTGTCTGGAGGGGTTCTAAGAGTCTCGGTTCATCTGCACAGATTTTTGCTGGTGCATTCCCTAAGAATTATGAACCTCCTACTGATAATTTCTACTTTGAAGTTAATGACGAATCCCCTATTGTGCAAGATGATATGGATCTGTTTGACTACAATGTGCCCGATCGCGTTAATGAGTTTGTATCTGCTGCAATATCACAAGCAAACATTACTCGTACAAATCATATCATGTGGACCATGGGAACGGACTTCAAGTATCAGTATGCTAATTCATGGTTCAGGCAGATGGATAAGTTCATTCATTATGTGAATCAAGATGGACGTGTCAATGCCCTATACTCAACCCCATCAATATACACTGATGCCAAGTATGCAGCAAATGAATCCTGGCCGATCAAGAGTGACGACTTCTTTCCATATGCTGACAAAGTGAATGCTTACTGGACTGGATATTTTACAAGCAGGCCAGCCATAAAAGGCTATGTTAGAGCTTTGAGCGGCTACTATTTGGCAGCAAGACAATTGGAGTTTTTTAAGGGAATGAGTAAATCGAGGCCTAACACAGATTCTTTGGCTGATGCATTAGCAATTGCTCAACATCATGATGCAGTTAGTGGCACAGAGAAACAGCATGTGGCTGATGACTATGCAAAACGACTTTCAATAGGTTACAATGAGGCTGAGAAGGTTGTTGCAGAATCACTTGCTTGCATGACAGAGTCGAGATCAGAAGCTGGCTGCAAGAGTCCATCAACTAAGTTTCAACAGTGTCCACTTCTGAATATTAGCTATTGTCCCCCATCAGAAGCTGATCTGTCAAATGGGAAATCGCTGGTTATTGTTGTCTATAACTCTCTTGGATGGAAGAGAGAGGATATCATCAAGATTCCTGTTGTCAGTGCAAATGTCACTGTAAGGGACTTTACAGGAAAAGAAATTGAATCACAACTCCTACCTCTGCTTAATGCGTCAGTCGGCATAAGAAATGACCATGTTAGGGCATATCTGGGAATATCTCCAAGTGTGACCCCAAGTTATTGGCTTACATTTTCAGCAACTGTACCACCTCTTGGTTTTAGCACTTACATTGTCTCAAGTGCCACACAGACAGCTACTAGTTCAGCGAGACGAACAGTATACAAGTCAGAAGCAAGTCAAAATGATACCATAGAAGTTGGGCCAGGAAACTTGAAACTTATTTATTCTGGAAATAAAGGGAAACTTACTCAATACTTTAACAGTAGAAGCTCGGTCAAAGAGTCAATAGAACAATCATTCAGCTATTACGCTGGAGATGATGGAAGTGTGGATAAACAGGCTGATGGAGCATACATCTTCCGCCCTAATGGTACATATCCCATCCAGTCTGAAGGACAGGATCATTTAACTGTTCTGAGGGGACCATTGCTAGATGAAGTACATCAGAGGATCAACTCTTGGATATATCAGGTCACTAGAGTGTACAAGGAAAAAGAGCATGCTGAGATTGAATTTACAGTTGGGCCTATACCAATTGGTGATGGAATTGGAAAAGAGATTGTAACTAAGATTACAACCAGCATGGAAACTAACAAAACATTCTACACAGATTCCAACGGGCGTGATTTTATTGAAAGAATTCGAGACTATAGAAAAGACTGGGACTTGCAAGTGAATCAACCTGTTGCAGGAAATTATTATCCGATTAACCTCGGAATCTATGCGAAAGATAACAACACAGAGATGTCAGTACTAGTAGATAGATCTGTAGGTGGATCCAGCATTGTGGATGGGCAATTGGAACTGATGGTTCACAGGAGGTTGCTCCATGATGATGACAGAGGTGTTGAAGAACCACTAAATGAAACTGTGTGCATTCAAGATATTTGCAAAGGACTAACAATCACGGGAAAGTACTATCTCAGACTCGATCCTCTAGGAGAGGGTGCTAAATGGCGTCGATCATTTGGTCAGGAGATATATTCTCCATTTCTTTTAGCCTTTACAGAACAGGAAGGAGATAACTGGACAAGTTCTCATGTGACAACCTTTTCATGGATGGATCCTTCCTACGTTTTACCTGATAATGTTGCAATAATAACCCTCCAGGAGCTGGAGGATGGGAAACTTCTGTTTCGTCTCGCACATTTATACGAGATTGAAGAGGATAAGGATCTTTCAGTTATGGCAAGTGTAGAGCTCAAAAAGGTGTTTGCAGATAAGAAGATAAACAAAGTGGCAGAAATGAGTTTATCTGCTAACCAAGAAAGAGCAGAGATGGAGAAGAAGAGACTGACGTGGAAAGTGGAAGGCTCCTCTGAAGAAGAAGATGCGGCCAAAGTGATGAGGGGAGGGCCTGTTGATCCAACAAAGCTGGTGGTGGATGTTGCTCCAATGGAAATTCGAACATTCATCATCGACTTCTATTCCAAGCATTAG

Protein Analysis

1015

Amino Acids

114.72

Weight (kDa)

5.37

Isoelectric Point (pI)

40.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 89, 147
AccB7I CCANNNNNTGG 3 cut(s) 488, 1697, 2719
AccBSI CCGCTC 1 cut(s) 1125
AccI GTMKAC 1 cut(s) 1764
AccII CGCG 3 cut(s) 91, 577, 795
AciI CCGC 6 cut(s) 240, 262, 343, 1125, 1966, 2939
AclI AACGTT 2 cut(s) 114, 298
AcoI YGGCCR 3 cut(s) 249, 1016, 2940
AcsI RAATTY 4 cut(s) 467, 2117, 2244, 3006
AcuI CTGAAG 2 cut(s) 2016, 2946
AcyI GRCGYC 3 cut(s) 86, 144, 2563
AfiI CCNNNNNNNGG 8 cut(s) 334, 488, 541, 1624, 1697, 2294, 2661, 2719
AflIII ACRYGT 1 cut(s) 949
AhlI ACTAGT 2 cut(s) 1742, 2359
AjiI CACGTC 2 cut(s) 950, 2904
AjnI CCWGG 3 cut(s) 1013, 1807, 2712
AjuI GAANNNNNNNTTGG 2 cut(s) 2109, 2141
AleI CACNNNNGTG 1 cut(s) 2084
AloI GAACNNNNNNTCC 2 cut(s) 195, 227
Alw21I GWGCWC 1 cut(s) 2808
Alw26I GTCTC 6 cut(s) 656, 1723, 1747, 2244, 2750, 2890
AlwNI CAGNNNCTG 4 cut(s) 1361, 1436, 1685, 2719
Ama87I CYCGRG 2 cut(s) 101, 443
AoxI GGCC 9 cut(s) 249, 543, 1016, 1091, 1181, 1805, 2129, 2940, 2960
ApeKI GCWGC 6 cut(s) 25, 404, 815, 1001, 1139, 1365
ApoI RAATTY 4 cut(s) 467, 2117, 2244, 3006
Asp700I GAANNNNTTC 1 cut(s) 2244
AspLEI GCGC 3 cut(s) 7, 93, 577
AspS9I GGNCC 6 cut(s) 543, 861, 1805, 2027, 2129, 2960
AsuC2I CCSGG 2 cut(s) 102, 103
AsuHPI GGTGA 1 cut(s) 2156
AsuII TTCGAA 1 cut(s) 3010
AvaI CYCGRG 2 cut(s) 101, 443
AvaII GGWCC 2 cut(s) 861, 2027
BaeGI GKGCMC 1 cut(s) 789
BamHI GGATCC 2 cut(s) 2381, 2668
BanII GRGCYC 1 cut(s) 2808
BbsI GAAGAC 1 cut(s) 164
Bbv12I GWGCWC 1 cut(s) 2808
BbvI GCAGC 6 cut(s) 12, 391, 802, 1013, 1151, 1352
BciT130I CCWGG 3 cut(s) 1015, 1809, 2714
BcnI CCSGG 2 cut(s) 102, 103
BcoDI GTCTC 6 cut(s) 656, 1723, 1747, 2244, 2750, 2890
BcuI ACTAGT 2 cut(s) 1742, 2359
BfaI CTAG 6 cut(s) 347, 1743, 2036, 2082, 2360, 2543
BfmI CTRYAG 3 cut(s) 519, 2254, 2373
BfoI RGCGCY 1 cut(s) 8
BglII AGATCT 1 cut(s) 2369
BisI GCNGC 9 cut(s) 26, 344, 405, 816, 1002, 1126, 1140, 1366, 2940
BlsI GCNGC 9 cut(s) 27, 345, 406, 817, 1003, 1127, 1141, 1367, 2941
BmcAI AGTACT 3 cut(s) 173, 2358, 2522
Bme1390I CCNGG 5 cut(s) 102, 103, 1015, 1809, 2714
Bme18I GGWCC 2 cut(s) 861, 2027
BmeT110I CYCGRG 2 cut(s) 101, 443
BmgBI CACGTC 2 cut(s) 950, 2904
BmgT120I GGNCC 6 cut(s) 543, 861, 1805, 2027, 2129, 2960
BmiI GGNNCC 7 cut(s) 237, 325, 544, 2028, 2383, 2670, 2920
BmrFI CCNGG 5 cut(s) 102, 103, 1015, 1809, 2714
BmrI ACTGGG 1 cut(s) 2275
BmsI GCATC 5 cut(s) 457, 979, 1195, 1222, 2926
BmuI ACTGGG 1 cut(s) 2275
BpiI GAAGAC 1 cut(s) 164
BpmI CTGGAG 4 cut(s) 655, 1938, 2696, 2741
Bpu14I TTCGAA 1 cut(s) 3010
BpuEI CTTGAG 2 cut(s) 644, 1705
BpuMI CCSGG 2 cut(s) 102, 103
Bsa29I ATCGAT 2 cut(s) 420, 429
BsaAI YACGTR 1 cut(s) 70
BsaBI GATNNNNATC 2 cut(s) 765, 2778
BsaHI GRCGYC 3 cut(s) 86, 144, 2563
BsaJI CCNNGG 6 cut(s) 100, 101, 442, 546, 864, 2317
BsaXI ACNNNNNCTCC 4 cut(s) 195, 225, 2949, 2979
Bsc4I CCNNNNNNNGG 8 cut(s) 334, 488, 541, 1624, 1697, 2294, 2661, 2719
Bse1I ACTGG 7 cut(s) 169, 369, 1073, 1078, 1990, 2270, 2636
Bse3DI GCAATG 2 cut(s) 242, 2030
Bse8I GATNNNNATC 2 cut(s) 765, 2778
BseBI CCWGG 3 cut(s) 1015, 1809, 2714
BseCI ATCGAT 2 cut(s) 420, 429
BseDI CCNNGG 6 cut(s) 100, 101, 442, 546, 864, 2317
BseGI GGATG 8 cut(s) 155, 390, 1493, 1986, 2401, 2670, 2731, 2995
BseJI GATNNNNATC 2 cut(s) 765, 2778
BseLI CCNNNNNNNGG 8 cut(s) 334, 488, 541, 1624, 1697, 2294, 2661, 2719
BseMI GCAATG 2 cut(s) 242, 2030
BseMII CTCAG 4 cut(s) 1299, 2012, 2100, 2542
BseNI ACTGG 7 cut(s) 169, 369, 1073, 1078, 1990, 2270, 2636
BseRI GAGGAG 2 cut(s) 399, 2911
BseSI GKGCMC 1 cut(s) 789
BseXI GCAGC 6 cut(s) 12, 391, 802, 1013, 1151, 1352
BsgI GTGCAG 3 cut(s) 92, 545, 648
Bsh1236I CGCG 3 cut(s) 91, 577, 795
Bsh1285I CGRYCG 1 cut(s) 793
BshFI GGCC 9 cut(s) 251, 545, 1018, 1093, 1183, 1807, 2131, 2942, 2962
BshVI ATCGAT 2 cut(s) 420, 429
BsiEI CGRYCG 1 cut(s) 793
BsiHKAI GWGCWC 1 cut(s) 2808
BsiHKCI CYCGRG 2 cut(s) 101, 443
BsiSI CCGG 1 cut(s) 102
BslFI GGGAC 6 cut(s) 83, 110, 1407, 1559, 2040, 2282
BslI CCNNNNNNNGG 8 cut(s) 334, 488, 541, 1624, 1697, 2294, 2661, 2719
BsmAI GTCTC 6 cut(s) 656, 1723, 1747, 2244, 2750, 2890
BsmBI CGTCTC 2 cut(s) 1747, 2750
BsmFI GGGAC 6 cut(s) 83, 110, 1407, 1559, 2040, 2282
BsmI GAATGC 3 cut(s) 683, 1066, 2481
BsnI GGCC 9 cut(s) 251, 545, 1018, 1093, 1183, 1807, 2131, 2942, 2962
BsoBI CYCGRG 2 cut(s) 101, 443
Bsp119I TTCGAA 1 cut(s) 3010
Bsp1286I GDGCHC 2 cut(s) 789, 2808
Bsp1407I TGTACA 1 cut(s) 2088
Bsp19I CCATGG 1 cut(s) 864
BspACI CCGC 6 cut(s) 240, 262, 343, 1125, 1966, 2939
BspANI GGCC 9 cut(s) 251, 545, 1018, 1093, 1183, 1807, 2131, 2942, 2962
BspCNI CTCAG 4 cut(s) 1300, 2013, 2101, 2541
BspDI ATCGAT 2 cut(s) 420, 429
BspFNI CGCG 3 cut(s) 91, 577, 795
BspHI TCATGA 1 cut(s) 1228
BspLI GGNNCC 7 cut(s) 237, 325, 544, 2028, 2383, 2670, 2920
BspMAI CTGCAG 1 cut(s) 523
BspT104I TTCGAA 1 cut(s) 3010
BsrBI CCGCTC 1 cut(s) 1125
BsrDI GCAATG 2 cut(s) 242, 2030
BsrGI TGTACA 1 cut(s) 2088
BsrI ACTGG 7 cut(s) 169, 369, 1073, 1078, 1990, 2270, 2636
BssECI CCNNGG 6 cut(s) 100, 101, 442, 546, 864, 2317
BssNAI GTATAC 1 cut(s) 1765
BssNI GRCGYC 3 cut(s) 86, 144, 2563
BssT1I CCWWGG 1 cut(s) 864
Bst1107I GTATAC 1 cut(s) 1765
Bst2UI CCWGG 3 cut(s) 1015, 1809, 2714
Bst6I CTCTTC 3 cut(s) 1487, 2763, 2888
BstACI GRCGYC 3 cut(s) 86, 144, 2563
BstAUI TGTACA 1 cut(s) 2088
BstBAI YACGTR 1 cut(s) 70
BstBI TTCGAA 1 cut(s) 3010
BstDEI CTNAG 8 cut(s) 645, 690, 1308, 1383, 2021, 2109, 2172, 2528
BstDSI CCRYGG 1 cut(s) 864
BstENI CCTNNNNNAGG 1 cut(s) 2292
BstF5I GGATG 8 cut(s) 155, 390, 1493, 1986, 2401, 2670, 2731, 2995
BstFNI CGCG 3 cut(s) 91, 577, 795
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 3 cut(s) 7, 93, 577
BstMAI GTCTC 6 cut(s) 656, 1723, 1747, 2244, 2750, 2890
BstMCI CGRYCG 1 cut(s) 793
BstMWI GCNNNNNNNGC 6 cut(s) 11, 31, 248, 527, 998, 1952
BstNI CCWGG 3 cut(s) 1015, 1809, 2714
BstNSI RCATGY 3 cut(s) 394, 1262, 2108
BstSCI CCNGG 5 cut(s) 100, 101, 1013, 1807, 2712
BstSFI CTRYAG 3 cut(s) 519, 2254, 2373
BstSLI GKGCMC 1 cut(s) 789
BstUI CGCG 3 cut(s) 91, 577, 795
BstV1I GCAGC 6 cut(s) 12, 391, 802, 1013, 1151, 1352
BstV2I GAAGAC 1 cut(s) 164
BstX2I RGATCY 5 cut(s) 766, 2369, 2381, 2668, 2779
BstXI CCANNNNNNTGG 2 cut(s) 1665, 2392
BstYI RGATCY 5 cut(s) 766, 2369, 2381, 2668, 2779
BstZ17I GTATAC 1 cut(s) 1765
Bsu15I ATCGAT 2 cut(s) 420, 429
BsuRI GGCC 9 cut(s) 251, 545, 1018, 1093, 1183, 1807, 2131, 2942, 2962
BsuTUI ATCGAT 2 cut(s) 420, 429
BtgI CCRYGG 1 cut(s) 864
BtrI CACGTC 2 cut(s) 950, 2904
BtsCI GGATG 8 cut(s) 155, 390, 1493, 1986, 2401, 2670, 2731, 2995
BtsI GCAGTG 1 cut(s) 528
BtsIMutI CAGTG 5 cut(s) 528, 984, 1400, 1531, 1536
CaiI CAGNNNCTG 4 cut(s) 1361, 1436, 1685, 2719
CciI TCATGA 1 cut(s) 1228
CfoI GCGC 3 cut(s) 7, 93, 577
Cfr13I GGNCC 6 cut(s) 543, 861, 1805, 2027, 2129, 2960
Cfr9I CCCGGG 1 cut(s) 101
ClaI ATCGAT 2 cut(s) 420, 429
CseI GACGC 2 cut(s) 1584, 2552
CspCI CAANNNNNGTGG 4 cut(s) 284, 319, 2964, 2999
DdeI CTNAG 8 cut(s) 645, 690, 1308, 1383, 2021, 2109, 2172, 2528
EaeI YGGCCR 3 cut(s) 249, 1016, 2940
Eam1104I CTCTTC 3 cut(s) 1487, 2763, 2888
EarI CTCTTC 3 cut(s) 1487, 2763, 2888
EciI GGCGGA 1 cut(s) 1955
Ecl136II GAGCTC 1 cut(s) 2806
Eco130I CCWWGG 1 cut(s) 864
Eco147I AGGCCT 1 cut(s) 1183
Eco24I GRGCYC 1 cut(s) 2808
Eco32I GATATC 1 cut(s) 1504
Eco47I GGWCC 2 cut(s) 861, 2027
Eco53kI GAGCTC 1 cut(s) 2806
Eco57I CTGAAG 2 cut(s) 2016, 2946
Eco88I CYCGRG 2 cut(s) 101, 443
EcoICRI GAGCTC 1 cut(s) 2806
EcoNI CCTNNNNNAGG 1 cut(s) 2292
EcoO109I RGGNCCY 1 cut(s) 2960
EcoRI GAATTC 2 cut(s) 467, 2244
EcoRII CCWGG 3 cut(s) 1013, 1807, 2712
EcoRV GATATC 1 cut(s) 1504
EcoT14I CCWWGG 1 cut(s) 864
EcoT22I ATGCAT 2 cut(s) 396, 1210
EcoT38I GRGCYC 1 cut(s) 2808
ErhI CCWWGG 1 cut(s) 864
Esp3I CGTCTC 2 cut(s) 1747, 2750
FalI AAGNNNNNCTT 2 cut(s) 1833, 1865
FaqI GGGAC 6 cut(s) 83, 110, 1407, 1559, 2040, 2282
FauI CCCGC 1 cut(s) 247
FauNDI CATATG 1 cut(s) 1045
FblI GTMKAC 1 cut(s) 1764
Fnu4HI GCNGC 9 cut(s) 26, 344, 405, 816, 1002, 1126, 1140, 1366, 2940
FokI GGATG 8 cut(s) 162, 397, 1500, 1973, 2408, 2677, 2738, 3002
FriOI GRGCYC 1 cut(s) 2808
Fsp4HI GCNGC 9 cut(s) 26, 344, 405, 816, 1002, 1126, 1140, 1366, 2940
FspBI CTAG 6 cut(s) 347, 1743, 2036, 2082, 2360, 2543
GlaI GCGC 3 cut(s) 6, 92, 576
GluI GCNGC 9 cut(s) 26, 344, 405, 816, 1002, 1126, 1140, 1366, 2940
GsuI CTGGAG 4 cut(s) 655, 1938, 2696, 2741
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 9 cut(s) 251, 545, 1018, 1093, 1183, 1807, 2131, 2942, 2962
HapII CCGG 1 cut(s) 102
HgaI GACGC 2 cut(s) 1584, 2552
HhaI GCGC 3 cut(s) 7, 93, 577
Hin1I GRCGYC 3 cut(s) 86, 144, 2563
Hin6I GCGC 3 cut(s) 5, 91, 575
HinP1I GCGC 3 cut(s) 5, 91, 575
HincII GTYRAC 3 cut(s) 166, 352, 362
HindII GTYRAC 3 cut(s) 166, 352, 362
HindIII AAGCTT 1 cut(s) 12
HpaII CCGG 1 cut(s) 102
HphI GGTGA 1 cut(s) 2156
Hpy166II GTNNAC 8 cut(s) 166, 352, 362, 861, 1400, 1765, 2089, 2419
Hpy8I GTNNAC 8 cut(s) 166, 352, 362, 861, 1400, 1765, 2089, 2419
Hpy99I CGWCG 6 cut(s) 88, 91, 143, 146, 149, 2568
HpyAV CCTTC 5 cut(s) 1306, 1991, 2615, 2682, 2909
HpyCH4IV ACGT 9 cut(s) 69, 86, 114, 144, 219, 298, 949, 2679, 2903
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 31, 248, 527, 998, 1952
HpyF3I CTNAG 8 cut(s) 645, 690, 1308, 1383, 2021, 2109, 2172, 2528
HpySE526I ACGT 9 cut(s) 69, 86, 114, 144, 219, 298, 949, 2679, 2903
Hsp92I GRCGYC 3 cut(s) 86, 144, 2563
HspAI GCGC 3 cut(s) 5, 91, 575
LmnI GCTCC 7 cut(s) 203, 412, 1952, 2436, 2716, 2924, 3001
Lsp1109I GCAGC 6 cut(s) 12, 391, 802, 1013, 1151, 1352
LweI GCATC 5 cut(s) 457, 979, 1195, 1222, 2926
MaeI CTAG 6 cut(s) 347, 1743, 2036, 2082, 2360, 2543
MaeII ACGT 9 cut(s) 69, 86, 114, 144, 219, 298, 949, 2679, 2903
MaeIII GTNAC 7 cut(s) 1028, 1295, 1534, 1651, 2077, 2167, 2647
MbiI CCGCTC 1 cut(s) 1125
MfeI CAATTG 4 cut(s) 1148, 1215, 2139, 2402
MflI RGATCY 5 cut(s) 766, 2369, 2381, 2668, 2779
MhlI GDGCHC 2 cut(s) 789, 2808
MlyI GAGTC 7 cut(s) 472, 554, 658, 1355, 1381, 1895, 2526
MmeI TCCRAC 3 cut(s) 127, 2244, 2996
Mph1103I ATGCAT 2 cut(s) 396, 1210
MroXI GAANNNNTTC 1 cut(s) 2244
MseI TTAA 8 cut(s) 459, 732, 798, 1161, 1590, 1863, 2012, 2313
MslI CAYNNNNRTG 5 cut(s) 422, 1230, 2084, 2348, 2439
MspI CCGG 1 cut(s) 102
MspR9I CCNGG 5 cut(s) 102, 103, 1015, 1809, 2714
MunI CAATTG 4 cut(s) 1148, 1215, 2139, 2402
Mva1269I GAATGC 3 cut(s) 683, 1066, 2481
MvaI CCWGG 3 cut(s) 1015, 1809, 2714
MvnI CGCG 3 cut(s) 91, 577, 795
MwoI GCNNNNNNNGC 6 cut(s) 11, 31, 248, 527, 998, 1952
NciI CCSGG 2 cut(s) 102, 103
NcoI CCATGG 1 cut(s) 864
NdeI CATATG 1 cut(s) 1045
NlaIV GGNNCC 7 cut(s) 237, 325, 544, 2028, 2383, 2670, 2920
NmeAIII GCCGAG 1 cut(s) 571
NmuCI GTSAC 5 cut(s) 1028, 1534, 1651, 2077, 2647
NsiI ATGCAT 2 cut(s) 396, 1210
NspI RCATGY 3 cut(s) 394, 1262, 2108
NspV TTCGAA 1 cut(s) 3010
OliI CACNNNNGTG 1 cut(s) 2084
PaeI GCATGC 2 cut(s) 394, 2108
PagI TCATGA 1 cut(s) 1228
PceI AGGCCT 1 cut(s) 1183
PcsI WCGNNNNNNNCGW 1 cut(s) 426
PctI GAATGC 3 cut(s) 683, 1066, 2481
PdmI GAANNNNTTC 1 cut(s) 2244
PflFI GACNNNGTC 2 cut(s) 143, 1345
PflMI CCANNNNNTGG 3 cut(s) 488, 1697, 2719
PfoI TCCNGGA 1 cut(s) 2712
PkrI GCNGC 9 cut(s) 27, 345, 406, 817, 1003, 1127, 1141, 1367, 2941
Ple19I CGATCG 1 cut(s) 793
PleI GAGTC 7 cut(s) 472, 554, 657, 1354, 1380, 1894, 2526
PpsI GAGTC 7 cut(s) 472, 554, 657, 1354, 1380, 1894, 2526
Ppu21I YACGTR 1 cut(s) 70
Psp124BI GAGCTC 1 cut(s) 2808
Psp1406I AACGTT 2 cut(s) 114, 298
Psp6I CCWGG 3 cut(s) 1013, 1807, 2712
PspGI CCWGG 3 cut(s) 1013, 1807, 2712
PspN4I GGNNCC 7 cut(s) 237, 325, 544, 2028, 2383, 2670, 2920
PspPI GGNCC 6 cut(s) 543, 861, 1805, 2027, 2129, 2960
PstI CTGCAG 1 cut(s) 523
PstNI CAGNNNCTG 4 cut(s) 1361, 1436, 1685, 2719
PsuI RGATCY 5 cut(s) 766, 2369, 2381, 2668, 2779
PsyI GACNNNGTC 2 cut(s) 143, 1345
PvuI CGATCG 1 cut(s) 793
RseI CAYNNNNRTG 5 cut(s) 422, 1230, 2084, 2348, 2439
SacI GAGCTC 1 cut(s) 2808
SaqAI TTAA 8 cut(s) 459, 732, 798, 1161, 1590, 1863, 2012, 2313
SatI GCNGC 9 cut(s) 26, 344, 405, 816, 1002, 1126, 1140, 1366, 2940
Sau96I GGNCC 6 cut(s) 543, 861, 1805, 2027, 2129, 2960
ScaI AGTACT 3 cut(s) 173, 2358, 2522
SchI GAGTC 7 cut(s) 472, 554, 658, 1355, 1381, 1895, 2526
ScrFI CCNGG 5 cut(s) 102, 103, 1015, 1809, 2714
SduI GDGCHC 2 cut(s) 789, 2808
SfaNI GCATC 5 cut(s) 457, 979, 1195, 1222, 2926
SfcI CTRYAG 3 cut(s) 519, 2254, 2373
SfuI TTCGAA 1 cut(s) 3010
SinI GGWCC 2 cut(s) 861, 2027
SmaI CCCGGG 1 cut(s) 103
SmiMI CAYNNNNRTG 5 cut(s) 422, 1230, 2084, 2348, 2439
SmlI CTYRAG 2 cut(s) 623, 1720
SmoI CTYRAG 2 cut(s) 623, 1720
SpeI ACTAGT 2 cut(s) 1742, 2359
SphI GCATGC 2 cut(s) 394, 2108
SseBI AGGCCT 1 cut(s) 1183
SsiI CCGC 6 cut(s) 240, 262, 343, 1125, 1966, 2939
SspI AATATT 1 cut(s) 1411
SspMI CTAG 6 cut(s) 347, 1743, 2036, 2082, 2360, 2543
SstI GAGCTC 1 cut(s) 2808
StuI AGGCCT 1 cut(s) 1183
StyD4I CCNGG 5 cut(s) 100, 101, 1013, 1807, 2712
StyI CCWWGG 1 cut(s) 864
TaiI ACGT 9 cut(s) 72, 89, 117, 147, 222, 301, 952, 2682, 2906
TaqII GACCGA 1 cut(s) 1867
TatI WGTACW 6 cut(s) 75, 171, 2044, 2088, 2356, 2520
TauI GCSGC 3 cut(s) 346, 1128, 2942
Tru1I TTAA 8 cut(s) 459, 732, 798, 1161, 1590, 1863, 2012, 2313
Tru9I TTAA 8 cut(s) 459, 732, 798, 1161, 1590, 1863, 2012, 2313
TscAI CASTG 5 cut(s) 528, 991, 1400, 1531, 1543
TseFI GTSAC 5 cut(s) 1028, 1534, 1651, 2077, 2647
TseI GCWGC 6 cut(s) 25, 404, 815, 1001, 1139, 1365
Tsp45I GTSAC 5 cut(s) 1028, 1534, 1651, 2077, 2647
TspGWI ACGGA 2 cut(s) 619, 887
TspMI CCCGGG 1 cut(s) 101
TspRI CASTG 5 cut(s) 528, 991, 1400, 1531, 1543
Tth111I GACNNNGTC 2 cut(s) 143, 1345
Van91I CCANNNNNTGG 3 cut(s) 488, 1697, 2719
VpaK11BI GGWCC 2 cut(s) 861, 2027
XagI CCTNNNNNAGG 1 cut(s) 2292
XapI RAATTY 4 cut(s) 467, 2117, 2244, 3006
XceI RCATGY 3 cut(s) 394, 1262, 2108
XcmI CCANNNNNNNNNTGG 2 cut(s) 176, 489
XmaI CCCGGG 1 cut(s) 101
XmiI GTMKAC 1 cut(s) 1764
XmnI GAANNNNTTC 1 cut(s) 2244
XspI CTAG 6 cut(s) 347, 1743, 2036, 2082, 2360, 2543
ZraI GACGTC 2 cut(s) 87, 145
ZrmI AGTACT 3 cut(s) 173, 2358, 2522
Zsp2I ATGCAT 2 cut(s) 396, 1210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.