Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0001301

Physical Location & Seq

Genomic Coordinates Reverse (-)

61249394 .. 61255760

Gene Structure 6367 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000004662

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2736 bp

ATGGCAGTCGTGGCTTGCTTTAGGCTCGTACTAGTATTCACTGTGTTTCTATATGCGGCAGAGTCGAAGTACATTCAGTACAATACGACGTCGGTCCTTGTTCCCGGGAAGCTGAACGTTCATTTGGTCGCTCACAGCCACGACGACGTCGGATGGCTGAAGACCATTGACCAGTACTATGTTGGCTCCAATAATTCCATCCAGGGAGCTTGTGTTCAGAACGTGCTGGATTCTTTGGTTCCGGCACTGTTAGCCGATAAGAACCGCAAGTTCATATACGTTGAGCAGGCATTTTTCCAAAGATGGTGGAGAGATCAGAGCGAGGAGGTGCAAGACACAGTGAAGGAGCTGGTCAGCTCTGGTCAACTGGAGCTCATAAATGGGGGGATGTGTATGCACGAGGAGGCGGCTGCCCATTACATTGATATGATTGATCAGACAACTCTGGGGCATCGATTTATTAAGCAGGAATTCAATTTGACTCCTAGAATCGGTTGGCAAATTGATCCATTTGGACATTCTGCTGTGCAGGCTTACCTTTTGGGGGCTGAGGTTGGATTTGACTCTCTTTTCTTTGCGCGAATTGATTACCAAGACAGAGCTAAACGAAAAGTTGACAAAAGCCTTGAGGTTGTGTGGCGGGGTTCTAAGAGTCTAGGTTCATCTGCGCAGATTTTCGCTGGTGCATTTCCTGCAAATTATGAACCTCCAAGTGATAATTTCTACTTTGAAGTTAATGATGCTGCCCCTATTGTACAAGATGATATGGATTTGTTTGACTACAATGTGCCGGACCGCGTTAATGATTTCGTATCTGCTGCTTTGTCACAAGCGAACATTACTCGGACAAATCATATCATGTGGACCATGGGAACTGATTTTAAGTATCAATATGCTCATACATGGTTTCGTCAGATGGATAAATTCATTAATTATGTGAATCAACAGCACGTGGCTAATGATTATGCAAAACGGCTTTCAATAGGTTATGTGGAGGCAGAGAAAGTTGTCGCAGAATCACTTTCTTGCATGACAGAGCCATCACAAGCTGGTTGCAAGAGTGTTGTAACCAAGTTTCAACAGTGTCCCCTTCTGAATATTAGCTATTGTCCATCGTCAGAAGTTGATCTTTCAAAGGGGAAAGACCTGGTTATTGTTGTCTATAATTCTCTGGGATGGAAAAGAAAGGATGTGATAAAGATTCCTGTTGTCAGTGAAAATGTCATTGTAAAGGACTCTACTGGAAAGGAAATTGAATCACAGATTCTACCCCTGCTTAATGCATCCCTAAGCATAAGAAACAACCTTGTTAAGGCATATCTGGGTATATCTTCGAGTGTGACACCAAGTTATTGGCTTGCATTTTCAGCAACTGTACCACCTCTTGGCTTTAGCACTTACATTGTCTCAAGTGCCAATCAGACAGTTGCAGCTACTACTTCAAAGAGACTTATTACAGAGTACAAGACAGAACTAAGTCAAGATACTGAAATCGAAGTGGGGCCAGGGAACTTGAAACTCATTTATTCTGGAAATGATGGAAAACTAATTGAATACACAAACAGCAGAAGCTCGGTCAAAGAGTCGGTAGATCAATCATTTAGCTACTACCCTGGAGATGATGGAAAGCATGCAAATAAATCTGATTTACAGGCCTCTGGAGCTTATATCTTCCGTCCTAATGGTACATATCCCATTAACTTTGAAGGGAAGGTTTCTCTTACTGTTTTGAGGGGTCCATTGTTAGATGAAGTACACCAGAGGATCAACTCTTGGATATACCAGGTCACTAGACTGTACAAGGAAAAAGAGCATGCTGAGATTGAATTTACTGTTGGGCCTATCCCAATTGATGATGGAATTGGCAAAGAGATTGTAACGAAGATTACAACCAGCATGAAGACTAACAAAAAATTCTACACAGATTCTAATGGGCGTGATTTTATTGAAAGAATTCGAGACTATAGAAAAGACTGGGACCTGGAAGTGAATCAACCTGTTGCTGGAAATTATTATCCGATTAATCTGGGAATGTACACGAAAGATAACAGCAAAGAGCTGTCAGTATTAGTAGATAGATCTGTGGGTGGATCCAGCATTGTGGATGGACAATTGGAACTTATGATTCATAGGAGGTTGATCTATGATGATGCTCGAGGTGTTGGAGAAGCACTAAATGAAACTGTGTGCATTCAAGATGATTGCAAAGGACTAACAGTCACAGGAAAGTACTATCTCAGACTTGATCCTATAGGAGAGGGTGCTAAATGGCGTCGATCAGTTGGTCAGGAGATATACTCTCCATTTCTTTTAGCCTTCACAGAACAGGATGGAGATCAGTGGACAAAATCTCATGTCACAACCTTTTCAGGAATGGATCCTTCCTACAGTTTACCAGATAATGTTGCAATACTAACCATCCAGGAGCTGGAAGATGGAAAAGTTCTCTTTCGCCTGGCACATTTATACGAGATTGAAGAGGACAAGGATCTTGCGGTTATGGCAAGTGTAGAACTAAAAAAAGTGTTTGCAGACAAGAAGATAAAGCAAATAACAGAAATGAGCTTATCTGCTAATCAAGGAAGAGCTGAAATGGAGAAGAAGAGATTGGTCTGGAAAGTAGAAGGCTCCTCTGAAGAAGAACCAAAAGTGTCGAGGGGAGGACCTGTTGATCCAACAAAGCTGGTGGTGGATCTTGCTCCAATGGAAATTCGAACATTTACGATCAACTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Alpha-mann_mid Glyco_hydro_38 Glyco_hydro_38C

Protein Analysis

912

Amino Acids

102.6

Weight (kDa)

5.4

Isoelectric Point (pI)

37.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	39 - 318	2.4e-73	Glycosyl hydrolases family 38 N-terminal domain
Laman-like_dom	PF21260	418 - 469	3.4e-09	Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C	PF07748	502 - 719	4.9e-45	Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2	PF17677	801 - 907	1.3e-07	Glycosyl hydrolases family 38 C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001301)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13980 AT5G13980 AT5G13980
fragaria_vesca	FvH4_5g09420 FvH4_5g09421 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272
malus_domestica	MD06G1131600.v1.1 MD09G1002900.v1.1 MD17G1007000.v1.1
prunus_persica	Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.5G142600_v2.0.a1 Prupe.5G142600_v2.0.a1
pyrus_communis	pycom06g12300 pycom06g12310 pycom111g00240 pycom17g00260
rosa_chinensis	RchiOBHm_Chr2g0176471 RchiOBHm_Chr2g0176481 RchiOBHm_Chr7g0188771 RchiOBHm_Chr7g0188781
rosa_laevigata	RLG00000004661 RLG00000004662 RLG00000022416
rosa_multiflora	Rmu_sc0000303.1_g000057 Rmu_sc0003642.1_g000008 Rmu_sc0011904.1_g000012
rosa_roxburghii	Rroxscaffold_2G00076370 Rroxscaffold_3G00266040 Rroxscaffold_3G00266050
rosa_rugosa	Rorug02G0595000 Rorug06G0493700
rosa_samantha	Rh2AG673300 Rh2BG684600 Rh2CG647400 Rh2DG698200 Rh7AG098300 Rh7AG098400 Rh7BG099900 Rh7BG100200 Rh7CG101600 Rh7CG101700 Rh7DG100100 Rh7DG100200
rosa_wichuraiana	Rw0G010390 Rw2G055220 Rw7G008480 Rw7G008490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	92, 150
Acc16I	TGCGCA	1 cut(s)	669
AccB7I	CCANNNNNTGG	2 cut(s)	1385, 2428
AccII	CGCG	2 cut(s)	580, 798
AciI	CCGC	6 cut(s)	56, 265, 407, 640, 796, 2495
AclI	AACGTT	1 cut(s)	117
AcsI	RAATTY	6 cut(s)	470, 923, 1826, 1913, 1953, 2709
AcuI	CTGAAG	2 cut(s)	179, 2655
AcvI	CACGTG	1 cut(s)	952
AcyI	GRCGYC	3 cut(s)	89, 147, 2272
AfiI	CCNNNNNNNGG	6 cut(s)	491, 544, 1312, 1385, 2003, 2428
AhdI	GACNNNNNGTC	1 cut(s)	2216
AhlI	ACTAGT	1 cut(s)	31
AjnI	CCWGG	8 cut(s)	201, 1146, 1504, 1612, 1782, 1980, 2421, 2454
AjuI	GAANNNNNNNTTGG	6 cut(s)	107, 139, 1818, 1850, 2591, 2623
AloI	GAACNNNNNNTCC	2 cut(s)	198, 230
Alw21I	GWGCWC	1 cut(s)	375
Alw26I	GTCTC	3 cut(s)	1411, 1441, 1953
AlwNI	CAGNNNCTG	2 cut(s)	1373, 2428
Ama87I	CYCGRG	2 cut(s)	104, 2154
AoxI	GGCC	3 cut(s)	1502, 1653, 1838
ApeKI	GCWGC	4 cut(s)	410, 743, 818, 1430
ApoI	RAATTY	6 cut(s)	470, 923, 1826, 1913, 1953, 2709
AseI	ATTAAT	2 cut(s)	930, 2022
Asp700I	GAANNNNTTC	1 cut(s)	1953
AspLEI	GCGC	2 cut(s)	580, 670
AspS9I	GGNCC	8 cut(s)	94, 793, 864, 1502, 1736, 1838, 1978, 2663
AsuC2I	CCSGG	2 cut(s)	105, 106
AsuII	TTCGAA	1 cut(s)	2713
AvaI	CYCGRG	2 cut(s)	104, 2154
AvaII	GGWCC	6 cut(s)	94, 793, 864, 1736, 1978, 2663
BamHI	GGATCC	2 cut(s)	2090, 2377
BanII	GRGCYC	1 cut(s)	375
BauI	CACGAG	1 cut(s)	398
BbrPI	CACGTG	1 cut(s)	952
BbsI	GAAGAC	2 cut(s)	167, 1907
Bbv12I	GWGCWC	1 cut(s)	375
BbvCI	CCTCAGC	1 cut(s)	549
BbvI	GCAGC	4 cut(s)	397, 730, 805, 1442
BceAI	ACGGC	1 cut(s)	989
BciT130I	CCWGG	8 cut(s)	203, 1148, 1506, 1614, 1784, 1982, 2423, 2456
BclI	TGATCA	1 cut(s)	433
BcnI	CCSGG	2 cut(s)	105, 106
BcoDI	GTCTC	3 cut(s)	1411, 1441, 1953
BcuI	ACTAGT	1 cut(s)	31
BfaI	CTAG	5 cut(s)	32, 486, 656, 1791, 2734
BfmI	CTRYAG	3 cut(s)	1963, 2250, 2386
BglII	AGATCT	1 cut(s)	2078
BisI	GCNGC	6 cut(s)	57, 408, 411, 744, 819, 1431
BlsI	GCNGC	6 cut(s)	58, 409, 412, 745, 820, 1432
BmcAI	AGTACT	2 cut(s)	176, 2231
Bme18I	GGWCC	6 cut(s)	94, 793, 864, 1736, 1978, 2663
BmeRI	GACNNNNNGTC	1 cut(s)	2216
BmeT110I	CYCGRG	2 cut(s)	104, 2154
BmgT120I	GGNCC	8 cut(s)	94, 793, 864, 1502, 1736, 1838, 1978, 2663
BmiI	GGNNCC	8 cut(s)	187, 240, 1503, 1737, 1979, 2092, 2379, 2629
BmrI	ACTGGG	1 cut(s)	1984
BmsI	GCATC	4 cut(s)	460, 730, 1292, 2140
BmuI	ACTGGG	1 cut(s)	1984
BoxI	GACNNNNGTC	1 cut(s)	92
BpiI	GAAGAC	2 cut(s)	167, 1907
BplI	GAGNNNNNCTC	2 cut(s)	2282, 2314
BpmI	CTGGAG	3 cut(s)	389, 1635, 1680
Bpu10I	CCTNAGC	2 cut(s)	549, 1289
Bpu14I	TTCGAA	1 cut(s)	2713
BpuEI	CTTGAG	2 cut(s)	647, 1393
BpuMI	CCSGG	2 cut(s)	105, 106
Bsa29I	ATCGAT	1 cut(s)	454
BsaAI	YACGTR	1 cut(s)	952
BsaBI	GATNNNNATC	1 cut(s)	2334
BsaHI	GRCGYC	3 cut(s)	89, 147, 2272
BsaJI	CCNNGG	5 cut(s)	104, 202, 867, 1505, 1612
BsaXI	ACNNNNNCTCC	4 cut(s)	198, 228, 2652, 2682
Bsc4I	CCNNNNNNNGG	6 cut(s)	491, 544, 1312, 1385, 2003, 2428
Bse1I	ACTGG	4 cut(s)	172, 372, 1246, 1979
Bse8I	GATNNNNATC	1 cut(s)	2334
BseBI	CCWGG	8 cut(s)	203, 1148, 1506, 1614, 1784, 1982, 2423, 2456
BseCI	ATCGAT	1 cut(s)	454
BseDI	CCNNGG	5 cut(s)	104, 202, 867, 1505, 1612
BseGI	GGATG	9 cut(s)	158, 198, 393, 1181, 1195, 1283, 2110, 2335, 2418
BseJI	GATNNNNATC	1 cut(s)	2334
BseLI	CCNNNNNNNGG	6 cut(s)	491, 544, 1312, 1385, 2003, 2428
BseMII	CTCAG	3 cut(s)	540, 1809, 2251
BseNI	ACTGG	4 cut(s)	172, 372, 1246, 1979
BseRI	GAGGAG	3 cut(s)	338, 416, 2620
BseXI	GCAGC	4 cut(s)	397, 730, 805, 1442
BsgI	GTGCAG	1 cut(s)	548
Bsh1236I	CGCG	2 cut(s)	580, 798
BshFI	GGCC	3 cut(s)	1504, 1655, 1840
BshVI	ATCGAT	1 cut(s)	454
BsiHKAI	GWGCWC	1 cut(s)	375
BsiHKCI	CYCGRG	2 cut(s)	104, 2154
BsiSI	CCGG	3 cut(s)	105, 242, 791
BslFI	GGGAC	2 cut(s)	1071, 1991
BslI	CCNNNNNNNGG	6 cut(s)	491, 544, 1312, 1385, 2003, 2428
BsmAI	GTCTC	3 cut(s)	1411, 1441, 1953
BsmFI	GGGAC	2 cut(s)	1071, 1991
BsmI	GAATGC	1 cut(s)	2190
BsnI	GGCC	3 cut(s)	1504, 1655, 1840
BsoBI	CYCGRG	2 cut(s)	104, 2154
Bsp119I	TTCGAA	1 cut(s)	2713
Bsp1286I	GDGCHC	1 cut(s)	375
Bsp1407I	TGTACA	3 cut(s)	754, 1797, 2034
Bsp19I	CCATGG	1 cut(s)	867
BspACI	CCGC	6 cut(s)	56, 265, 407, 640, 796, 2495
BspANI	GGCC	3 cut(s)	1504, 1655, 1840
BspCNI	CTCAG	3 cut(s)	541, 1810, 2250
BspDI	ATCGAT	1 cut(s)	454
BspFNI	CGCG	2 cut(s)	580, 798
BspLI	GGNNCC	8 cut(s)	187, 240, 1503, 1737, 1979, 2092, 2379, 2629
BspQI	GCTCTTC	1 cut(s)	2578
BspT104I	TTCGAA	1 cut(s)	2713
BsrGI	TGTACA	3 cut(s)	754, 1797, 2034
BsrI	ACTGG	4 cut(s)	172, 372, 1246, 1979
BssECI	CCNNGG	5 cut(s)	104, 202, 867, 1505, 1612
BssNI	GRCGYC	3 cut(s)	89, 147, 2272
BssSI	CACGAG	1 cut(s)	398
BssT1I	CCWWGG	1 cut(s)	867
Bst2BI	CACGAG	1 cut(s)	398
Bst2UI	CCWGG	8 cut(s)	203, 1148, 1506, 1614, 1784, 1982, 2423, 2456
Bst6I	CTCTTC	3 cut(s)	2472, 2578, 2597
BstACI	GRCGYC	3 cut(s)	89, 147, 2272
BstAPI	GCANNNNNTGC	1 cut(s)	692
BstAUI	TGTACA	3 cut(s)	754, 1797, 2034
BstBAI	YACGTR	1 cut(s)	952
BstBI	TTCGAA	1 cut(s)	2713
BstC8I	GCNNGC	6 cut(s)	16, 288, 531, 1359, 1632, 1815
BstDEI	CTNAG	6 cut(s)	549, 648, 1289, 1475, 1818, 2237
BstDSI	CCRYGG	1 cut(s)	867
BstENI	CCTNNNNNAGG	1 cut(s)	1310
BstF5I	GGATG	9 cut(s)	158, 198, 393, 1181, 1195, 1283, 2110, 2335, 2418
BstFNI	CGCG	2 cut(s)	580, 798
BstHHI	GCGC	2 cut(s)	580, 670
BstMAI	GTCTC	3 cut(s)	1411, 1441, 1953
BstMWI	GCNNNNNNNGC	7 cut(s)	11, 251, 530, 692, 1367, 1661, 2501
BstNI	CCWGG	8 cut(s)	203, 1148, 1506, 1614, 1784, 1982, 2423, 2456
BstNSI	RCATGY	2 cut(s)	1634, 1817
BstPAI	GACNNNNGTC	1 cut(s)	92
BstSFI	CTRYAG	3 cut(s)	1963, 2250, 2386
BstUI	CGCG	2 cut(s)	580, 798
BstV1I	GCAGC	4 cut(s)	397, 730, 805, 1442
BstV2I	GAAGAC	2 cut(s)	167, 1907
BstX2I	RGATCY	5 cut(s)	2078, 2090, 2377, 2488, 2692
BstXI	CCANNNNNNTGG	2 cut(s)	1353, 2101
BstYI	RGATCY	5 cut(s)	2078, 2090, 2377, 2488, 2692
Bsu15I	ATCGAT	1 cut(s)	454
BsuRI	GGCC	3 cut(s)	1504, 1655, 1840
BsuTUI	ATCGAT	1 cut(s)	454
BtgI	CCRYGG	1 cut(s)	867
BtsCI	GGATG	9 cut(s)	158, 198, 393, 1181, 1195, 1283, 2110, 2335, 2418
BtsIMutI	CAGTG	6 cut(s)	39, 245, 345, 1088, 1219, 2345
Cac8I	GCNNGC	6 cut(s)	16, 288, 531, 1359, 1632, 1815
CaiI	CAGNNNCTG	2 cut(s)	1373, 2428
CfoI	GCGC	2 cut(s)	580, 670
Cfr13I	GGNCC	8 cut(s)	94, 793, 864, 1502, 1736, 1838, 1978, 2663
Cfr9I	CCCGGG	1 cut(s)	104
ClaI	ATCGAT	1 cut(s)	454
CpoI	CGGWCCG	1 cut(s)	793
CseI	GACGC	1 cut(s)	2261
CsiI	ACCWGGT	2 cut(s)	1146, 1782
CspCI	CAANNNNNGTGG	4 cut(s)	287, 322, 2667, 2702
CspI	CGGWCCG	1 cut(s)	793
CviAII	CATG	8 cut(s)	859, 868, 903, 1030, 1631, 1814, 1897, 2354
DdeI	CTNAG	6 cut(s)	549, 648, 1289, 1475, 1818, 2237
DriI	GACNNNNNGTC	1 cut(s)	2216
Eam1104I	CTCTTC	3 cut(s)	2472, 2578, 2597
Eam1105I	GACNNNNNGTC	1 cut(s)	2216
EarI	CTCTTC	3 cut(s)	2472, 2578, 2597
Ecl136II	GAGCTC	1 cut(s)	373
Eco130I	CCWWGG	1 cut(s)	867
Eco147I	AGGCCT	1 cut(s)	1655
Eco24I	GRGCYC	1 cut(s)	375
Eco47I	GGWCC	6 cut(s)	94, 793, 864, 1736, 1978, 2663
Eco53kI	GAGCTC	1 cut(s)	373
Eco57I	CTGAAG	2 cut(s)	179, 2655
Eco72I	CACGTG	1 cut(s)	952
Eco88I	CYCGRG	2 cut(s)	104, 2154
EcoICRI	GAGCTC	1 cut(s)	373
EcoNI	CCTNNNNNAGG	1 cut(s)	1310
EcoO109I	RGGNCCY	2 cut(s)	1978, 2663
EcoRI	GAATTC	2 cut(s)	470, 1953
EcoRII	CCWGG	8 cut(s)	201, 1146, 1504, 1612, 1782, 1980, 2421, 2454
EcoT14I	CCWWGG	1 cut(s)	867
EcoT22I	ATGCAT	1 cut(s)	1285
EcoT38I	GRGCYC	1 cut(s)	375
ErhI	CCWWGG	1 cut(s)	867
FaeI	CATG	8 cut(s)	862, 871, 906, 1033, 1634, 1817, 1900, 2357
FalI	AAGNNNNNCTT	2 cut(s)	1113, 1145
FaqI	GGGAC	2 cut(s)	1071, 1991
FatI	CATG	8 cut(s)	858, 867, 902, 1029, 1630, 1813, 1896, 2353
FauI	CCCGC	1 cut(s)	633
FbaI	TGATCA	1 cut(s)	433
Fnu4HI	GCNGC	6 cut(s)	57, 408, 411, 744, 819, 1431
FokI	GGATG	9 cut(s)	165, 185, 400, 1188, 1202, 1270, 2117, 2342, 2405
FriOI	GRGCYC	1 cut(s)	375
Fsp4HI	GCNGC	6 cut(s)	57, 408, 411, 744, 819, 1431
FspBI	CTAG	5 cut(s)	32, 486, 656, 1791, 2734
FspI	TGCGCA	1 cut(s)	669
GlaI	GCGC	2 cut(s)	579, 669
GluI	GCNGC	6 cut(s)	57, 408, 411, 744, 819, 1431
GsuI	CTGGAG	3 cut(s)	389, 1635, 1680
HaeIII	GGCC	3 cut(s)	1504, 1655, 1840
HapII	CCGG	3 cut(s)	105, 242, 791
HgaI	GACGC	1 cut(s)	2261
HhaI	GCGC	2 cut(s)	580, 670
Hin1I	GRCGYC	3 cut(s)	89, 147, 2272
Hin1II	CATG	8 cut(s)	862, 871, 906, 1033, 1634, 1817, 1900, 2357
Hin6I	GCGC	2 cut(s)	578, 668
HinP1I	GCGC	2 cut(s)	578, 668
HincII	GTYRAC	2 cut(s)	365, 616
HindII	GTYRAC	2 cut(s)	365, 616
HpaII	CCGG	3 cut(s)	105, 242, 791
Hpy166II	GTNNAC	7 cut(s)	365, 616, 864, 1756, 2037, 2343, 2393
Hpy188III	TCNNGA	8 cut(s)	1481, 1530, 1659, 1958, 2195, 2288, 2370, 2614
Hpy8I	GTNNAC	7 cut(s)	365, 616, 864, 1756, 2037, 2343, 2393
Hpy99I	CGWCG	6 cut(s)	91, 94, 146, 149, 152, 2277
HpyAV	CCTTC	7 cut(s)	337, 1100, 1700, 1705, 2326, 2391, 2618
HpyCH4IV	ACGT	6 cut(s)	89, 117, 147, 222, 279, 951
HpyF10VI	GCNNNNNNNGC	7 cut(s)	11, 251, 530, 692, 1367, 1661, 2501
HpyF3I	CTNAG	6 cut(s)	549, 648, 1289, 1475, 1818, 2237
HpySE526I	ACGT	6 cut(s)	89, 117, 147, 222, 279, 951
Hsp92I	GRCGYC	3 cut(s)	89, 147, 2272
Hsp92II	CATG	8 cut(s)	862, 871, 906, 1033, 1634, 1817, 1900, 2357
HspAI	GCGC	2 cut(s)	578, 668
Ksp22I	TGATCA	1 cut(s)	433
LguI	GCTCTTC	1 cut(s)	2578
LmnI	GCTCC	8 cut(s)	191, 206, 346, 370, 1661, 2425, 2633, 2704
Lsp1109I	GCAGC	4 cut(s)	397, 730, 805, 1442
LweI	GCATC	4 cut(s)	460, 730, 1292, 2140
MabI	ACCWGGT	2 cut(s)	1146, 1782
MaeI	CTAG	5 cut(s)	32, 486, 656, 1791, 2734
MaeII	ACGT	6 cut(s)	89, 117, 147, 222, 279, 951
MaeIII	GTNAC	7 cut(s)	825, 1066, 1339, 1786, 1876, 2218, 2356
MfeI	CAATTG	2 cut(s)	1848, 2111
MflI	RGATCY	5 cut(s)	2078, 2090, 2377, 2488, 2692
MhlI	GDGCHC	1 cut(s)	375
MlyI	GAGTC	6 cut(s)	71, 475, 557, 661, 1229, 1592
MmeI	TCCRAC	4 cut(s)	130, 535, 2143, 2699
Mph1103I	ATGCAT	1 cut(s)	1285
MroXI	GAANNNNTTC	1 cut(s)	1953
MseI	TTAA	9 cut(s)	462, 735, 801, 882, 930, 1278, 1311, 1698, 2022
MslI	CAYNNNNRTG	1 cut(s)	425
MspI	CCGG	3 cut(s)	105, 242, 791
MunI	CAATTG	2 cut(s)	1848, 2111
Mva1269I	GAATGC	1 cut(s)	2190
MvaI	CCWGG	8 cut(s)	203, 1148, 1506, 1614, 1784, 1982, 2423, 2456
MvnI	CGCG	2 cut(s)	580, 798
MwoI	GCNNNNNNNGC	7 cut(s)	11, 251, 530, 692, 1367, 1661, 2501
NciI	CCSGG	2 cut(s)	105, 106
NcoI	CCATGG	1 cut(s)	867
NlaIII	CATG	8 cut(s)	862, 871, 906, 1033, 1634, 1817, 1900, 2357
NlaIV	GGNNCC	8 cut(s)	187, 240, 1503, 1737, 1979, 2092, 2379, 2629
NmuCI	GTSAC	5 cut(s)	825, 1339, 1786, 2218, 2356
NsbI	TGCGCA	1 cut(s)	669
NsiI	ATGCAT	1 cut(s)	1285
NspI	RCATGY	2 cut(s)	1634, 1817
NspV	TTCGAA	1 cut(s)	2713
PaeI	GCATGC	2 cut(s)	1634, 1817
PaeR7I	CTCGAG	1 cut(s)	2154
PceI	AGGCCT	1 cut(s)	1655
PciSI	GCTCTTC	1 cut(s)	2578
PctI	GAATGC	1 cut(s)	2190
PdmI	GAANNNNTTC	1 cut(s)	1953
PflFI	GACNNNGTC	1 cut(s)	146
PflMI	CCANNNNNTGG	2 cut(s)	1385, 2428
PfoI	TCCNGGA	1 cut(s)	2421
PkrI	GCNGC	6 cut(s)	58, 409, 412, 745, 820, 1432
PleI	GAGTC	6 cut(s)	70, 475, 557, 660, 1229, 1591
PmaCI	CACGTG	1 cut(s)	952
PmlI	CACGTG	1 cut(s)	952
PpsI	GAGTC	6 cut(s)	70, 475, 557, 660, 1229, 1591
Ppu21I	YACGTR	1 cut(s)	952
PpuMI	RGGWCCY	2 cut(s)	1978, 2663
PshAI	GACNNNNGTC	1 cut(s)	92
PshBI	ATTAAT	2 cut(s)	930, 2022
Psp124BI	GAGCTC	1 cut(s)	375
Psp1406I	AACGTT	1 cut(s)	117
Psp5II	RGGWCCY	2 cut(s)	1978, 2663
Psp6I	CCWGG	8 cut(s)	201, 1146, 1504, 1612, 1782, 1980, 2421, 2454
PspCI	CACGTG	1 cut(s)	952
PspGI	CCWGG	8 cut(s)	201, 1146, 1504, 1612, 1782, 1980, 2421, 2454
PspN4I	GGNNCC	8 cut(s)	187, 240, 1503, 1737, 1979, 2092, 2379, 2629
PspPI	GGNCC	8 cut(s)	94, 793, 864, 1502, 1736, 1838, 1978, 2663
PspPPI	RGGWCCY	2 cut(s)	1978, 2663
PspXI	VCTCGAGB	1 cut(s)	2154
PstNI	CAGNNNCTG	2 cut(s)	1373, 2428
PsuI	RGATCY	5 cut(s)	2078, 2090, 2377, 2488, 2692
PsyI	GACNNNGTC	1 cut(s)	146
RseI	CAYNNNNRTG	1 cut(s)	425
Rsr2I	CGGWCCG	1 cut(s)	793
RsrII	CGGWCCG	1 cut(s)	793
SacI	GAGCTC	1 cut(s)	375
SapI	GCTCTTC	1 cut(s)	2578
SaqAI	TTAA	9 cut(s)	462, 735, 801, 882, 930, 1278, 1311, 1698, 2022
SatI	GCNGC	6 cut(s)	57, 408, 411, 744, 819, 1431
Sau96I	GGNCC	8 cut(s)	94, 793, 864, 1502, 1736, 1838, 1978, 2663
ScaI	AGTACT	2 cut(s)	176, 2231
SchI	GAGTC	6 cut(s)	71, 475, 557, 661, 1229, 1592
SduI	GDGCHC	1 cut(s)	375
SexAI	ACCWGGT	2 cut(s)	1146, 1782
SfaNI	GCATC	4 cut(s)	460, 730, 1292, 2140
SfcI	CTRYAG	3 cut(s)	1963, 2250, 2386
Sfr274I	CTCGAG	1 cut(s)	2154
SfuI	TTCGAA	1 cut(s)	2713
SinI	GGWCC	6 cut(s)	94, 793, 864, 1736, 1978, 2663
SlaI	CTCGAG	1 cut(s)	2154
SmaI	CCCGGG	1 cut(s)	106
SmiMI	CAYNNNNRTG	1 cut(s)	425
SmlI	CTYRAG	3 cut(s)	626, 1408, 2154
SmoI	CTYRAG	3 cut(s)	626, 1408, 2154
SpeI	ACTAGT	1 cut(s)	31
SphI	GCATGC	2 cut(s)	1634, 1817
SseBI	AGGCCT	1 cut(s)	1655
SsiI	CCGC	6 cut(s)	56, 265, 407, 640, 796, 2495
SspI	AATATT	1 cut(s)	1099
SspMI	CTAG	5 cut(s)	32, 486, 656, 1791, 2734
SstI	GAGCTC	1 cut(s)	375
StuI	AGGCCT	1 cut(s)	1655
StyI	CCWWGG	1 cut(s)	867
TaiI	ACGT	6 cut(s)	92, 120, 150, 225, 282, 954
TaqI	TCGA	9 cut(s)	65, 454, 1334, 1494, 1957, 2155, 2275, 2654, 2713
TaqII	GACCGA	2 cut(s)	82, 1564
TatI	WGTACW	9 cut(s)	69, 78, 174, 754, 1461, 1753, 1797, 2034, 2229
TauI	GCSGC	2 cut(s)	59, 410
Tru1I	TTAA	9 cut(s)	462, 735, 801, 882, 930, 1278, 1311, 1698, 2022
Tru9I	TTAA	9 cut(s)	462, 735, 801, 882, 930, 1278, 1311, 1698, 2022
TscAI	CASTG	6 cut(s)	46, 252, 345, 1088, 1219, 2345
TseFI	GTSAC	5 cut(s)	825, 1339, 1786, 2218, 2356
TseI	GCWGC	4 cut(s)	410, 743, 818, 1430
Tsp45I	GTSAC	5 cut(s)	825, 1339, 1786, 2218, 2356
TspDTI	ATGAA	9 cut(s)	110, 262, 651, 717, 916, 1764, 1913, 2117, 2193
TspGWI	ACGGA	1 cut(s)	1664
TspMI	CCCGGG	1 cut(s)	104
TspRI	CASTG	6 cut(s)	46, 252, 345, 1088, 1219, 2345
Tth111I	GACNNNGTC	1 cut(s)	146
Van91I	CCANNNNNTGG	2 cut(s)	1385, 2428
VpaK11BI	GGWCC	6 cut(s)	94, 793, 864, 1736, 1978, 2663
VspI	ATTAAT	2 cut(s)	930, 2022
XagI	CCTNNNNNAGG	1 cut(s)	1310
XapI	RAATTY	6 cut(s)	470, 923, 1826, 1913, 1953, 2709
XceI	RCATGY	2 cut(s)	1634, 1817
XcmI	CCANNNNNNNNNTGG	2 cut(s)	179, 2425
XhoI	CTCGAG	1 cut(s)	2154
XmaI	CCCGGG	1 cut(s)	104
XmnI	GAANNNNTTC	1 cut(s)	1953
XspI	CTAG	5 cut(s)	32, 486, 656, 1791, 2734
ZraI	GACGTC	2 cut(s)	90, 148
ZrmI	AGTACT	2 cut(s)	176, 2231
Zsp2I	ATGCAT	1 cut(s)	1285

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.