Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0001301

Physical Location & Seq

Genomic Coordinates Reverse (-)

39660967 .. 39669547

Gene Structure 8581 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g54272.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3102 bp

ATGGCGACCAAGACTCCTGGTTTACTCTTCTTCTTGGCTCTTCTTCTTCTGGTCGCTGACTCGAAATTTATGGTATATGAGACCTCGCAAGGGATAGTCCCCGGCAAACTGAACGTGCACTTGGTCCCTCACACCCACGACGACGTCGGATGGTTGAAGACCGTTGATCAGTATTACGTTGGCTCCAACAATTCCATCCAGGGCGCTTGTGTTCAAAATGTGTTGGACTCTTTGATTCCTGCTCTGCTGGCTGACAAGAGTCGCAAATTTGTCTATGTTGAAATTGCCTTTTTCCAGCGCTGGTGGAGAGAGCAGAGTGATGCTGTCCAGCGTATAACCAAGGACCTTGTCAGCTCCGGTCAACTCGAGTTCATAAATGGGGGCATGTGTATGCATGATGAAGCAGCTACCCATTATGTTGACATGGTTGACCAGACCACTCTGGGGCACCGCTTTCTTAAAAGGGAATTCAATGTCACTCCAAGAATTGGATGGCAAATTGATCCTTTTGGACATTCGGCTGTCCAGGCCTACTTGTTGGGAGCTGAAGTTGGATTCGACTCACTTTTCTTTGGTCGAATAGATTACCAGGACAGGGAAAAACGCAAAAATGAGAAGAGTCTTGAGTTTGTTTGGCGGGGTTCTAAGAGTCTCGGTTCATCTGCGGAGATATTTTCTGGTGCTTTCCCTGAGAATTATGAACCTCCTTCTGGTTTCTATTTTGAAGTTAATGATCCTTCCCCAATAGTCCAGGATGACATCACTTTGTTTGATTACAATGTTCAAGAACGAGTTAATGACTTCATTGCGGCTGCAGTAAAACAGGCTAACATAACTCGAACAAATCATATTATGTGGACCATGGGAACAGATTTCAAGTATCAGTATGCACACACATGGTTCCGACAAATGGACAAGCTCATTCATTATGTCAACAAGGATGGACGTGTTAATGCTCTGTACTCTACTCCCTCCATATACACCGATGCAAAATATGCTTCAAATGAATCTTGGCCAATCAAGACTGATGACTTCTTTCCTTATGCAGATCGTATTAATGCTTACTGGACAGGATACTTCACAAGCAGACCAGCCTTGAAACACTATGTTCGAACAATGAGTGGCTATTATTTGGCTGCTAGGCAACTAGAATTTTTCAAAGGAAGGAGTAATTCAGGGCCTAATACAGACTCATTGGCAGATGCCTTGGCAATTGCTCAACATCATGATGCTGTCACTGGTACAGAAAAGCAGCATGTTGCCAATGATTACGCAAAAAGATTGTCAATAGGATACATGGAGTCTGAGGAACTAGTTGCAACTTCACTTGCTCACTTGGTTGAGTCTGCTTCTGGAAACCCAACAACAAAGTTTCAACAGTGTGTACTTCTGAATATAAGCTATTGTCCTTCATCAGAAGTAACTTTGTCTCAGGGGAAGAAATTGATTGTCGTGGTGTACAATTCTCTTGGATGGAAGAGGGATGACACAGTCCGAATTCCTGTTGTCAATGAAGCTATCATTGTTCATGATTCTGAGGGAAAAGAAGTCAAGTCACAGCTTCTTCCTCTAGATGATGTCCATCTGGGCTTAAGGAACCACTATGTTAAGGCATACTTGGGTCAATTTCCAAATAAGACACCAAAGTATTGGCTTGTGTTTACAGTATCCGTACCACCACTTGGATTCAACACATACACTATCTCGGGTGCCGAAGGGGCAGGTGCTAGCACGACCAGATCATCTGTATACACCATTCAAAGCAAGGAAAAGTCAACTATTGAAGTTGGTCAAGGAAATGTGAAACTCACTTTTTCCACAGATGAAGGAAAAATGATTAATTATGTCAATAGCAGAAGCTTGGTGGAAGAATCGGTTCAGCAGTCTTACAGCTTTTATAATGGGTATAATGGCAGTTATGATAAACCTCCTCTGATTCCTCAGAATGCTGGGGCATATATCTTCCGGCCTAATGGTTCATCTTTCGTAACACCTGAAGAAAAGGTTCCTTTGACCGTTATGCAAGGACCTGTAATAGATGAAGTACATCAACAGATTAATTCATGGATACATCAGGTCACCAGGCTTCACAAGGAGAAGGAGCATGTTGAAGTTGAATTTATTGTTGGGCCTATACCTATTAATGACGGAATCGGAAAAGAAGTTGTAACTCAACTGAACACTACCATGGCTACCAACAAAACATTCTATACTGATTCTAATGGGCGTGATTTTATCAAAAGGATTCGGGACTACAGAACAGATTGGGACTTCAAAGTGAACCAACCTGTTGCTGGAAATTATTATCCAGTATGTTTGTTTCTGTGTCTTCCTCACAGTATGATTAATCTTGGGATTTACATGCAAGATGATAAGAAAGAGTTTTCAGTTTTGGTGGATCGATCACTAGGAGGATCCAGTATAGTGGATGGTCAGATAGAGCTGATGCTTCACAGGAGACTACTACTTGATGACTCAAGAGGTGTTGCCGAGGCTCTAAATGAAACAGTTTGTGTTTCCGATGAATGCACAGGACTAAGAGTCCTAGGGAAACTATACTTCAGGATTGACCCCTTGGGAGATGGAGCTAAGTGGCGTCGTTCTTTTGGCCAGGAGATATATTCACCTCTTCTACTAGCGTTTACTGAACAGGATGGAGATGACTGGAAGAATTCTCATGAAACCACTTTTTCTGGGATCGATTCCTCATATAGTTTACCTGATAATGTTGCTCTTATAACTCTTCAGGAGCTTGATGATGGAAAAGTTCTCATTCGCTTAGCACATTTATACGAGGTTGGAGAGGACAAGGATTTTTCTGTAATGGCAAATGTAGAGCTGAAGAAGCTCTTCCCCAGGAAGAAGATTGGCAAAGTGACGGAAATGAACTTATCGGCTAATCAAGAGAGGACAGATATGGAGAGAAAGAGGCTAGTATGGAAAGCGGAAGAGGGGTCTTCAGGAACAGCAAAGGGTGTGAGGGGTGGGCCTGTTGATCCTGCAAAACTGGTTGTGGAACTTGCCCCAATGGAAATCCGCACCTTTCTTATCGACTTGGAGCAGAATTTGCAGCGCCACCATTATCATGTAGTGGATGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0001775 GO:0002252 GO:0002263 GO:0002274 GO:0002275 GO:0002283 GO:0002366 GO:0002376 GO:0002443 GO:0002444 GO:0002446 GO:0003674 GO:0003824 GO:0004553 GO:0004559 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005766 GO:0005773 GO:0005775 GO:0005975 GO:0005996 GO:0006013 GO:0006464 GO:0006517 GO:0006807 GO:0006810 GO:0006887 GO:0006955 GO:0008150 GO:0008152 GO:0009056 GO:0009100 GO:0009311 GO:0009313 GO:0009987 GO:0012505 GO:0015923 GO:0016052 GO:0016192 GO:0016787 GO:0016798 GO:0019318 GO:0019538 GO:0030141 GO:0031410 GO:0031974 GO:0031982 GO:0031983 GO:0032940 GO:0034774 GO:0035578 GO:0036211 GO:0036230 GO:0042119 GO:0042582 GO:0043170 GO:0043202 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043299 GO:0043312 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044281 GO:0044421 GO:0044422 GO:0044424 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045055 GO:0045321 GO:0046903 GO:0050896 GO:0051179 GO:0051234 GO:0060205 GO:0070013 GO:0071704 GO:0097708 GO:0099503 GO:1901135 GO:1901564 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Alpha-mann_mid Glyco_hydro_38 Glyco_hydro_38C

Protein Analysis

1034

Amino Acids

116.71

Weight (kDa)

5.85

Isoelectric Point (pI)

37.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	38 - 325	1.3e-80	Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid	PF09261	330 - 422	3.9e-21	Alpha mannosidase middle domain
Laman-like_dom	PF21260	493 - 544	7.6e-11	Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C	PF07748	573 - 792	9.4e-47	Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2	PF17677	874 - 980	1.6e-08	Glycosyl hydrolases family 38 C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001301)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13980 AT5G13980 AT5G13980
fragaria_vesca	FvH4_5g09420 FvH4_5g09421 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272
malus_domestica	MD06G1131600.v1.1 MD09G1002900.v1.1 MD17G1007000.v1.1
prunus_persica	Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.5G142600_v2.0.a1 Prupe.5G142600_v2.0.a1
pyrus_communis	pycom06g12300 pycom06g12310 pycom111g00240 pycom17g00260
rosa_chinensis	RchiOBHm_Chr2g0176471 RchiOBHm_Chr2g0176481 RchiOBHm_Chr7g0188771 RchiOBHm_Chr7g0188781
rosa_laevigata	RLG00000004661 RLG00000004662 RLG00000022416
rosa_multiflora	Rmu_sc0000303.1_g000057 Rmu_sc0003642.1_g000008 Rmu_sc0011904.1_g000012
rosa_roxburghii	Rroxscaffold_2G00076370 Rroxscaffold_3G00266040 Rroxscaffold_3G00266050
rosa_rugosa	Rorug02G0595000 Rorug06G0493700
rosa_samantha	Rh2AG673300 Rh2BG684600 Rh2CG647400 Rh2DG698200 Rh7AG098300 Rh7AG098400 Rh7BG099900 Rh7BG100200 Rh7CG101600 Rh7CG101700 Rh7DG100100 Rh7DG100200
rosa_wichuraiana	Rw0G010390 Rw2G055220 Rw7G008480 Rw7G008490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1899, 2738
AarI	CACCTGC	1 cut(s)	1712
AatII	GACGTC	1 cut(s)	147
Acc36I	ACCTGC	1 cut(s)	1712
AccB1I	GGYRCC	2 cut(s)	447, 1709
AccB7I	CCANNNNNTGG	2 cut(s)	488, 1682
AccI	GTMKAC	1 cut(s)	1749
AciI	CCGC	6 cut(s)	451, 637, 665, 809, 2945, 3037
AclWI	GGATC	7 cut(s)	497, 728, 2406, 2409, 2422, 2705, 2990
AcoI	YGGCCR	2 cut(s)	1013, 2608
AcsI	RAATTY	8 cut(s)	65, 266, 467, 1151, 1497, 2117, 2671, 3064
AcuI	CTGAAG	6 cut(s)	567, 2016, 2545, 2729, 2861, 2943
AcyI	GRCGYC	2 cut(s)	144, 2596
AfaI	GTAC	6 cut(s)	962, 1243, 1386, 1460, 1674, 2046
AfeI	AGCGCT	1 cut(s)	299
AfiI	CCNNNNNNNGG	8 cut(s)	90, 444, 488, 595, 710, 910, 1682, 2294
AflII	CTTAAG	1 cut(s)	1591
AflIII	ACRYGT	1 cut(s)	946
AhdI	GACNNNNNGTC	1 cut(s)	2540
AhlI	ACTAGT	1 cut(s)	1312
AjiI	CACGTC	1 cut(s)	947
AjnI	CCWGG	8 cut(s)	16, 198, 525, 588, 750, 2081, 2610, 2855
AjuI	GAANNNNNNNTTGG	6 cut(s)	104, 136, 2109, 2141, 2189, 2221
Alw21I	GWGCWC	1 cut(s)	120
Alw26I	GTCTC	4 cut(s)	74, 656, 1434, 2452
Alw44I	GTGCAC	1 cut(s)	116
AlwI	GGATC	7 cut(s)	497, 728, 2406, 2409, 2422, 2705, 2990
Ama87I	CYCGRG	2 cut(s)	365, 1705
Aor51HI	AGCGCT	1 cut(s)	299
AoxI	GGCC	7 cut(s)	528, 1013, 1178, 1967, 2129, 2608, 2987
ApaLI	GTGCAC	1 cut(s)	116
ApeKI	GCWGC	5 cut(s)	404, 812, 1136, 1252, 3070
ApoI	RAATTY	8 cut(s)	65, 266, 467, 1151, 1497, 2117, 2671, 3064
ArsI	GACNNNNNNTTYG	2 cut(s)	1268, 1300
AseI	ATTAAT	5 cut(s)	1056, 1839, 2058, 2142, 2346
Asp700I	GAANNNNTTC	3 cut(s)	1875, 2004, 2849
AspA2I	CCTAGG	1 cut(s)	2545
AspLEI	GCGC	3 cut(s)	206, 300, 3075
AspS9I	GGNCC	7 cut(s)	124, 343, 858, 1178, 2027, 2129, 2987
AsuC2I	CCSGG	1 cut(s)	102
AsuHPI	GGTGA	2 cut(s)	2071, 2616
AsuII	TTCGAA	1 cut(s)	1111
AsuNHI	GCTAGC	1 cut(s)	1727
AvaI	CYCGRG	2 cut(s)	365, 1705
AvaII	GGWCC	4 cut(s)	124, 343, 858, 2027
AvrII	CCTAGG	1 cut(s)	2545
BaeGI	GKGCMC	2 cut(s)	120, 450
BalI	TGGCCA	2 cut(s)	1015, 2610
BamHI	GGATCC	1 cut(s)	2414
BanI	GGYRCC	2 cut(s)	447, 1709
BbsI	GAAGAC	3 cut(s)	164, 2321, 2949
Bbv12I	GWGCWC	1 cut(s)	120
BbvI	GCAGC	5 cut(s)	416, 799, 1123, 1264, 3082
BciT130I	CCWGG	8 cut(s)	18, 200, 527, 590, 752, 2083, 2612, 2857
BciVI	GTATCC	4 cut(s)	1067, 1286, 1678, 2061
BclI	TGATCA	1 cut(s)	166
BcnI	CCSGG	1 cut(s)	102
BcoDI	GTCTC	4 cut(s)	74, 656, 1434, 2452
BcuI	ACTAGT	1 cut(s)	1312
BfaI	CTAG	9 cut(s)	1140, 1148, 1313, 1571, 1728, 2408, 2546, 2636, 2933
BfmI	CTRYAG	2 cut(s)	813, 2254
BfoI	RGCGCY	3 cut(s)	207, 301, 3076
BfrI	CTTAAG	1 cut(s)	1591
BfuAI	ACCTGC	1 cut(s)	1712
BfuI	GTATCC	4 cut(s)	1067, 1286, 1678, 2061
BglI	GCCNNNNNGGC	1 cut(s)	1718
BisI	GCNGC	6 cut(s)	405, 810, 813, 1137, 1253, 3071
BlnI	CCTAGG	1 cut(s)	2545
BlpI	GCTNAGC	1 cut(s)	2779
BlsI	GCNGC	6 cut(s)	406, 811, 814, 1138, 1254, 3072
Bme1390I	CCNGG	9 cut(s)	18, 102, 200, 527, 590, 752, 2083, 2612, 2857
Bme18I	GGWCC	4 cut(s)	124, 343, 858, 2027
BmeRI	GACNNNNNGTC	1 cut(s)	2540
BmeT110I	CYCGRG	2 cut(s)	365, 1705
BmgBI	CACGTC	1 cut(s)	947
BmgT120I	GGNCC	7 cut(s)	124, 343, 858, 1178, 2027, 2129, 2987
BmiI	GGNNCC	8 cut(s)	126, 184, 449, 902, 1598, 1711, 2007, 2416
BmrFI	CCNGG	9 cut(s)	18, 102, 200, 527, 590, 752, 2083, 2612, 2857
BmsI	GCATC	6 cut(s)	310, 976, 1192, 1219, 2436, 3085
BmtI	GCTAGC	1 cut(s)	1731
BoxI	GACNNNNGTC	1 cut(s)	258
BpiI	GAAGAC	3 cut(s)	164, 2321, 2949
Bpu1102I	GCTNAGC	1 cut(s)	2779
Bpu14I	TTCGAA	1 cut(s)	1111
BpuEI	CTTGAG	2 cut(s)	644, 2461
BpuMI	CCSGG	1 cut(s)	102
Bsa29I	ATCGAT	2 cut(s)	2401, 2700
BsaBI	GATNNNNATC	1 cut(s)	1581
BsaHI	GRCGYC	2 cut(s)	144, 2596
BsaI	GGTCTC	1 cut(s)	74
BsaWI	WCCGGW	1 cut(s)	356
BsaXI	ACNNNNNCTCC	5 cut(s)	28, 167, 197, 534, 564
Bsc4I	CCNNNNNNNGG	8 cut(s)	90, 444, 488, 595, 710, 910, 1682, 2294
Bse1I	ACTGG	6 cut(s)	1070, 1243, 2309, 2418, 2669, 3012
Bse3DI	GCAATG	1 cut(s)	804
Bse8I	GATNNNNATC	1 cut(s)	1581
BseBI	CCWGG	8 cut(s)	18, 200, 527, 590, 752, 2083, 2612, 2857
BseCI	ATCGAT	2 cut(s)	2401, 2700
BseJI	GATNNNNATC	1 cut(s)	1581
BseLI	CCNNNNNNNGG	8 cut(s)	90, 444, 488, 595, 710, 910, 1682, 2294
BseMI	GCAATG	1 cut(s)	804
BseMII	CTCAG	5 cut(s)	681, 1296, 1445, 1527, 1955
BseNI	ACTGG	6 cut(s)	1070, 1243, 2309, 2418, 2669, 3012
BseRI	GAGGAG	1 cut(s)	1920
BseSI	GKGCMC	2 cut(s)	120, 450
BseXI	GCAGC	5 cut(s)	416, 799, 1123, 1264, 3082
BseYI	CCCAGC	1 cut(s)	1949
BshFI	GGCC	7 cut(s)	530, 1015, 1180, 1969, 2131, 2610, 2989
BshNI	GGYRCC	2 cut(s)	447, 1709
BshVI	ATCGAT	2 cut(s)	2401, 2700
BsiHKAI	GWGCWC	1 cut(s)	120
BsiHKCI	CYCGRG	2 cut(s)	365, 1705
BsiSI	CCGG	3 cut(s)	102, 357, 1966
BslFI	GGGAC	4 cut(s)	83, 110, 2264, 2282
BslI	CCNNNNNNNGG	8 cut(s)	90, 444, 488, 595, 710, 910, 1682, 2294
BsmAI	GTCTC	4 cut(s)	74, 656, 1434, 2452
BsmFI	GGGAC	4 cut(s)	83, 110, 2264, 2282
BsmI	GAATGC	2 cut(s)	1951, 2531
BsnI	GGCC	7 cut(s)	530, 1015, 1180, 1969, 2131, 2610, 2989
Bso31I	GGTCTC	1 cut(s)	74
BsoBI	CYCGRG	2 cut(s)	365, 1705
Bsp119I	TTCGAA	1 cut(s)	1111
Bsp1286I	GDGCHC	2 cut(s)	120, 450
Bsp1407I	TGTACA	1 cut(s)	1458
Bsp1720I	GCTNAGC	1 cut(s)	2779
Bsp19I	CCATGG	2 cut(s)	861, 2187
BspACI	CCGC	6 cut(s)	451, 637, 665, 809, 2945, 3037
BspANI	GGCC	7 cut(s)	530, 1015, 1180, 1969, 2131, 2610, 2989
BspCNI	CTCAG	5 cut(s)	682, 1297, 1444, 1528, 1954
BspDI	ATCGAT	2 cut(s)	2401, 2700
BspHI	TCATGA	3 cut(s)	1225, 1528, 2677
BspLI	GGNNCC	8 cut(s)	126, 184, 449, 902, 1598, 1711, 2007, 2416
BspMAI	CTGCAG	1 cut(s)	817
BspMI	ACCTGC	1 cut(s)	1712
BspOI	GCTAGC	1 cut(s)	1731
BspPI	GGATC	7 cut(s)	497, 728, 2406, 2409, 2422, 2705, 2990
BspQI	GCTCTTC	2 cut(s)	45, 2855
BspT104I	TTCGAA	1 cut(s)	1111
BspT107I	GGYRCC	2 cut(s)	447, 1709
BspTI	CTTAAG	1 cut(s)	1591
BspTNI	GGTCTC	1 cut(s)	74
BsrDI	GCAATG	1 cut(s)	804
BsrGI	TGTACA	1 cut(s)	1458
BsrI	ACTGG	6 cut(s)	1070, 1243, 2309, 2418, 2669, 3012
BssNAI	GTATAC	1 cut(s)	1750
BssNI	GRCGYC	2 cut(s)	144, 2596
BssT1I	CCWWGG	6 cut(s)	339, 861, 1206, 2187, 2545, 2574
Bst1107I	GTATAC	1 cut(s)	1750
Bst2UI	CCWGG	8 cut(s)	18, 200, 527, 590, 752, 2083, 2612, 2857
Bst4CI	ACNGT	7 cut(s)	163, 1380, 1492, 1666, 2017, 2339, 2509
Bst6I	CTCTTC	8 cut(s)	32, 45, 611, 1472, 2634, 2748, 2855, 2943
BstACI	GRCGYC	2 cut(s)	144, 2596
BstAFI	CTTAAG	1 cut(s)	1591
BstAPI	GCANNNNNTGC	2 cut(s)	995, 3067
BstAUI	TGTACA	1 cut(s)	1458
BstBI	TTCGAA	1 cut(s)	1111
BstC8I	GCNNGC	2 cut(s)	249, 1729
BstDEI	CTNAG	9 cut(s)	645, 690, 1305, 1431, 1536, 1941, 2537, 2589, 2779
BstDSI	CCRYGG	2 cut(s)	861, 2187
BstEII	GGTNACC	1 cut(s)	2077
BstH2I	RGCGCY	3 cut(s)	207, 301, 3076
BstHHI	GCGC	3 cut(s)	206, 300, 3075
BstMAI	GTCTC	4 cut(s)	74, 656, 1434, 2452
BstMWI	GCNNNNNNNGC	6 cut(s)	248, 527, 995, 1718, 2845, 3067
BstNI	CCWGG	8 cut(s)	18, 200, 527, 590, 752, 2083, 2612, 2857
BstNSI	RCATGY	4 cut(s)	388, 1259, 2108, 2365
BstPAI	GACNNNNGTC	1 cut(s)	258
BstPI	GGTNACC	1 cut(s)	2077
BstSCI	CCNGG	9 cut(s)	16, 100, 198, 525, 588, 750, 2081, 2610, 2855
BstSFI	CTRYAG	2 cut(s)	813, 2254
BstSLI	GKGCMC	2 cut(s)	120, 450
BstV1I	GCAGC	5 cut(s)	416, 799, 1123, 1264, 3082
BstV2I	GAAGAC	3 cut(s)	164, 2321, 2949
BstX2I	RGATCY	1 cut(s)	2414
BstXI	CCANNNNNNTGG	2 cut(s)	1650, 2425
BstYI	RGATCY	1 cut(s)	2414
BstZ17I	GTATAC	1 cut(s)	1750
Bsu15I	ATCGAT	2 cut(s)	2401, 2700
BsuI	GTATCC	4 cut(s)	1067, 1286, 1678, 2061
BsuRI	GGCC	7 cut(s)	530, 1015, 1180, 1969, 2131, 2610, 2989
BsuTUI	ATCGAT	2 cut(s)	2401, 2700
BtgI	CCRYGG	2 cut(s)	861, 2187
BtrI	CACGTC	1 cut(s)	947
BtsIMutI	CAGTG	2 cut(s)	1236, 1385
BveI	ACCTGC	1 cut(s)	1712
Cac8I	GCNNGC	2 cut(s)	249, 1729
CciI	TCATGA	3 cut(s)	1225, 1528, 2677
CfoI	GCGC	3 cut(s)	206, 300, 3075
Cfr13I	GGNCC	7 cut(s)	124, 343, 858, 1178, 2027, 2129, 2987
ClaI	ATCGAT	2 cut(s)	2401, 2700
CseI	GACGC	1 cut(s)	2585
Csp6I	GTAC	6 cut(s)	961, 1242, 1385, 1459, 1673, 2045
CviQI	GTAC	6 cut(s)	961, 1242, 1385, 1459, 1673, 2045
DdeI	CTNAG	9 cut(s)	645, 690, 1305, 1431, 1536, 1941, 2537, 2589, 2779
DriI	GACNNNNNGTC	1 cut(s)	2540
EaeI	YGGCCR	2 cut(s)	1013, 2608
Eam1104I	CTCTTC	8 cut(s)	32, 45, 611, 1472, 2634, 2748, 2855, 2943
Eam1105I	GACNNNNNGTC	1 cut(s)	2540
EarI	CTCTTC	8 cut(s)	32, 45, 611, 1472, 2634, 2748, 2855, 2943
Eco130I	CCWWGG	6 cut(s)	339, 861, 1206, 2187, 2545, 2574
Eco147I	AGGCCT	1 cut(s)	530
Eco31I	GGTCTC	1 cut(s)	74
Eco47I	GGWCC	4 cut(s)	124, 343, 858, 2027
Eco47III	AGCGCT	1 cut(s)	299
Eco57I	CTGAAG	6 cut(s)	567, 2016, 2545, 2729, 2861, 2943
Eco88I	CYCGRG	2 cut(s)	365, 1705
Eco91I	GGTNACC	1 cut(s)	2077
EcoO109I	RGGNCCY	3 cut(s)	343, 1178, 2027
EcoO65I	GGTNACC	1 cut(s)	2077
EcoRI	GAATTC	3 cut(s)	467, 1497, 2671
EcoRII	CCWGG	8 cut(s)	16, 198, 525, 588, 750, 2081, 2610, 2855
EcoT14I	CCWWGG	6 cut(s)	339, 861, 1206, 2187, 2545, 2574
EcoT22I	ATGCAT	2 cut(s)	396, 3100
ErhI	CCWWGG	6 cut(s)	339, 861, 1206, 2187, 2545, 2574
FalI	AAGNNNNNCTT	4 cut(s)	1601, 1633, 2834, 2866
FaqI	GGGAC	4 cut(s)	83, 110, 2264, 2282
FauI	CCCGC	1 cut(s)	630
FbaI	TGATCA	1 cut(s)	166
FblI	GTMKAC	1 cut(s)	1749
Fnu4HI	GCNGC	6 cut(s)	405, 810, 813, 1137, 1253, 3071
FokI	GGATG	9 cut(s)	162, 182, 504, 767, 953, 1485, 1496, 2441, 2666
Fsp4HI	GCNGC	6 cut(s)	405, 810, 813, 1137, 1253, 3071
FspBI	CTAG	9 cut(s)	1140, 1148, 1313, 1571, 1728, 2408, 2546, 2636, 2933
GlaI	GCGC	3 cut(s)	205, 299, 3074
GluI	GCNGC	6 cut(s)	405, 810, 813, 1137, 1253, 3071
GsaI	CCCAGC	1 cut(s)	1953
HaeII	RGCGCY	3 cut(s)	207, 301, 3076
HaeIII	GGCC	7 cut(s)	530, 1015, 1180, 1969, 2131, 2610, 2989
HapII	CCGG	3 cut(s)	102, 357, 1966
HgaI	GACGC	1 cut(s)	2585
HhaI	GCGC	3 cut(s)	206, 300, 3075
Hin1I	GRCGYC	2 cut(s)	144, 2596
Hin6I	GCGC	3 cut(s)	204, 298, 3073
HinP1I	GCGC	3 cut(s)	204, 298, 3073
HincII	GTYRAC	5 cut(s)	362, 421, 430, 934, 1776
HindII	GTYRAC	5 cut(s)	362, 421, 430, 934, 1776
HindIII	AAGCTT	1 cut(s)	1858
HpaII	CCGG	3 cut(s)	102, 357, 1966
HphI	GGTGA	2 cut(s)	2071, 2616
Hpy99I	CGWCG	4 cut(s)	143, 146, 149, 2601
HpyAV	CCTTC	7 cut(s)	717, 747, 1158, 1419, 1709, 1820, 2092
HpyCH4III	ACNGT	7 cut(s)	163, 1380, 1492, 1666, 2017, 2339, 2509
HpyCH4IV	ACGT	4 cut(s)	114, 144, 177, 946
HpyF10VI	GCNNNNNNNGC	6 cut(s)	248, 527, 995, 1718, 2845, 3067
HpyF3I	CTNAG	9 cut(s)	645, 690, 1305, 1431, 1536, 1941, 2537, 2589, 2779
HpySE526I	ACGT	4 cut(s)	114, 144, 177, 946
Hsp92I	GRCGYC	2 cut(s)	144, 2596
HspAI	GCGC	3 cut(s)	204, 298, 3073
Ksp22I	TGATCA	1 cut(s)	166
LguI	GCTCTTC	2 cut(s)	45, 2855
LmnI	GCTCC	7 cut(s)	188, 359, 542, 2101, 2585, 2749, 3058
Lsp1109I	GCAGC	5 cut(s)	416, 799, 1123, 1264, 3082
LweI	GCATC	6 cut(s)	310, 976, 1192, 1219, 2436, 3085
MaeI	CTAG	9 cut(s)	1140, 1148, 1313, 1571, 1728, 2408, 2546, 2636, 2933
MaeII	ACGT	4 cut(s)	114, 144, 177, 946
MaeIII	GTNAC	8 cut(s)	475, 1234, 1420, 1554, 1987, 2077, 2167, 2875
MfeI	CAATTG	1 cut(s)	1212
MflI	RGATCY	1 cut(s)	2414
MhlI	GDGCHC	2 cut(s)	120, 450
MlsI	TGGCCA	2 cut(s)	1015, 2610
MluNI	TGGCCA	2 cut(s)	1015, 2610
MmeI	TCCRAC	6 cut(s)	127, 204, 210, 532, 928, 2779
Mox20I	TGGCCA	2 cut(s)	1015, 2610
Mph1103I	ATGCAT	2 cut(s)	396, 3100
MroXI	GAANNNNTTC	3 cut(s)	1875, 2004, 2849
MscI	TGGCCA	2 cut(s)	1015, 2610
MslI	CAYNNNNRTG	5 cut(s)	389, 895, 1227, 2186, 3084
Msp20I	TGGCCA	2 cut(s)	1015, 2610
MspCI	CTTAAG	1 cut(s)	1591
MspI	CCGG	3 cut(s)	102, 357, 1966
MspR9I	CCNGG	9 cut(s)	18, 102, 200, 527, 590, 752, 2083, 2612, 2857
MunI	CAATTG	1 cut(s)	1212
Mva1269I	GAATGC	2 cut(s)	1951, 2531
MvaI	CCWGG	8 cut(s)	18, 200, 527, 590, 752, 2083, 2612, 2857
MwoI	GCNNNNNNNGC	6 cut(s)	248, 527, 995, 1718, 2845, 3067
NciI	CCSGG	1 cut(s)	102
NcoI	CCATGG	2 cut(s)	861, 2187
NheI	GCTAGC	1 cut(s)	1727
NlaIV	GGNNCC	8 cut(s)	126, 184, 449, 902, 1598, 1711, 2007, 2416
NmeAIII	GCCGAG	1 cut(s)	2515
NmuCI	GTSAC	5 cut(s)	475, 1234, 1554, 2077, 2875
NsiI	ATGCAT	2 cut(s)	396, 3100
NspI	RCATGY	4 cut(s)	388, 1259, 2108, 2365
NspV	TTCGAA	1 cut(s)	1111
PaeR7I	CTCGAG	1 cut(s)	365
PagI	TCATGA	3 cut(s)	1225, 1528, 2677
PaqCI	CACCTGC	1 cut(s)	1712
PceI	AGGCCT	1 cut(s)	530
PciSI	GCTCTTC	2 cut(s)	45, 2855
PctI	GAATGC	2 cut(s)	1951, 2531
PdmI	GAANNNNTTC	3 cut(s)	1875, 2004, 2849
PflFI	GACNNNGTC	3 cut(s)	143, 347, 1490
PflMI	CCANNNNNTGG	2 cut(s)	488, 1682
PfoI	TCCNGGA	1 cut(s)	750
PkrI	GCNGC	6 cut(s)	406, 811, 814, 1138, 1254, 3072
PpuMI	RGGWCCY	2 cut(s)	343, 2027
PshAI	GACNNNNGTC	1 cut(s)	258
PshBI	ATTAAT	5 cut(s)	1056, 1839, 2058, 2142, 2346
PsiI	TTATAA	2 cut(s)	1899, 2738
Psp5II	RGGWCCY	2 cut(s)	343, 2027
Psp6I	CCWGG	8 cut(s)	16, 198, 525, 588, 750, 2081, 2610, 2855
PspEI	GGTNACC	1 cut(s)	2077
PspFI	CCCAGC	1 cut(s)	1949
PspGI	CCWGG	8 cut(s)	16, 198, 525, 588, 750, 2081, 2610, 2855
PspN4I	GGNNCC	8 cut(s)	126, 184, 449, 902, 1598, 1711, 2007, 2416
PspPI	GGNCC	7 cut(s)	124, 343, 858, 1178, 2027, 2129, 2987
PspPPI	RGGWCCY	2 cut(s)	343, 2027
PspXI	VCTCGAGB	1 cut(s)	365
PstI	CTGCAG	1 cut(s)	817
PsuI	RGATCY	1 cut(s)	2414
PsyI	GACNNNGTC	3 cut(s)	143, 347, 1490
RsaI	GTAC	6 cut(s)	962, 1243, 1386, 1460, 1674, 2046
RsaNI	GTAC	6 cut(s)	961, 1242, 1385, 1459, 1673, 2045
RseI	CAYNNNNRTG	5 cut(s)	389, 895, 1227, 2186, 3084
SapI	GCTCTTC	2 cut(s)	45, 2855
SatI	GCNGC	6 cut(s)	405, 810, 813, 1137, 1253, 3071
Sau96I	GGNCC	7 cut(s)	124, 343, 858, 1178, 2027, 2129, 2987
ScrFI	CCNGG	9 cut(s)	18, 102, 200, 527, 590, 752, 2083, 2612, 2857
SduI	GDGCHC	2 cut(s)	120, 450
SfaNI	GCATC	6 cut(s)	310, 976, 1192, 1219, 2436, 3085
SfcI	CTRYAG	2 cut(s)	813, 2254
Sfr274I	CTCGAG	1 cut(s)	365
SfuI	TTCGAA	1 cut(s)	1111
SinI	GGWCC	4 cut(s)	124, 343, 858, 2027
SlaI	CTCGAG	1 cut(s)	365
SmiMI	CAYNNNNRTG	5 cut(s)	389, 895, 1227, 2186, 3084
SmlI	CTYRAG	4 cut(s)	365, 623, 1591, 2476
SmoI	CTYRAG	4 cut(s)	365, 623, 1591, 2476
SpeI	ACTAGT	1 cut(s)	1312
SseBI	AGGCCT	1 cut(s)	530
SsiI	CCGC	6 cut(s)	451, 637, 665, 809, 2945, 3037
SspMI	CTAG	9 cut(s)	1140, 1148, 1313, 1571, 1728, 2408, 2546, 2636, 2933
StuI	AGGCCT	1 cut(s)	530
StyD4I	CCNGG	9 cut(s)	16, 100, 198, 525, 588, 750, 2081, 2610, 2855
StyI	CCWWGG	6 cut(s)	339, 861, 1206, 2187, 2545, 2574
TaaI	ACNGT	7 cut(s)	163, 1380, 1492, 1666, 2017, 2339, 2509
TaiI	ACGT	4 cut(s)	117, 147, 180, 949
TaqI	TCGA	9 cut(s)	62, 366, 558, 577, 838, 1111, 2401, 2700, 3051
TatI	WGTACW	4 cut(s)	960, 1384, 1458, 2044
TauI	GCSGC	1 cut(s)	812
TscAI	CASTG	2 cut(s)	1243, 1385
TseFI	GTSAC	5 cut(s)	475, 1234, 1554, 2077, 2875
TseI	GCWGC	5 cut(s)	404, 812, 1136, 1252, 3070
Tsp45I	GTSAC	5 cut(s)	475, 1234, 1554, 2077, 2875
TspGWI	ACGGA	3 cut(s)	1660, 2163, 2894
TspRI	CASTG	2 cut(s)	1243, 1385
Tth111I	GACNNNGTC	3 cut(s)	143, 347, 1490
Van91I	CCANNNNNTGG	2 cut(s)	488, 1682
Vha464I	CTTAAG	1 cut(s)	1591
VneI	GTGCAC	1 cut(s)	116
VpaK11BI	GGWCC	4 cut(s)	124, 343, 858, 2027
VspI	ATTAAT	5 cut(s)	1056, 1839, 2058, 2142, 2346
XapI	RAATTY	8 cut(s)	65, 266, 467, 1151, 1497, 2117, 2671, 3064
XbaI	TCTAGA	1 cut(s)	1570
XceI	RCATGY	4 cut(s)	388, 1259, 2108, 2365
XcmI	CCANNNNNNNNNTGG	2 cut(s)	489, 2291
XhoI	CTCGAG	1 cut(s)	365
XmaJI	CCTAGG	1 cut(s)	2545
XmiI	GTMKAC	1 cut(s)	1749
XmnI	GAANNNNTTC	3 cut(s)	1875, 2004, 2849
XspI	CTAG	9 cut(s)	1140, 1148, 1313, 1571, 1728, 2408, 2546, 2636, 2933
ZraI	GACGTC	1 cut(s)	145
Zsp2I	ATGCAT	2 cut(s)	396, 3100

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.