Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0001301

Physical Location & Seq

Genomic Coordinates Forward (+)

233742 .. 242009

Gene Structure 8268 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00076370.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3114 bp

ATGGCGACCACGACTCCTCGTTTACTCTTCTTCTTCTTCTTCTGTGTGCTTATCTCGGTCGGGGGGATTCTAGTCGCCGACTCCAAATTTATGGTCTATAACACCTCCCAAGGGATAGTCCCCGGTAAACTTAACGTCCACTTGGTCCCTCACACCCACGACGACGTCGGATGGTTGAAGACCGTTGATCAGTATTATGTTGGCTCCAACAATTCCATCCAGGGGGCTTGTGTGCAAAATGTGTTGGACTCTTTGATTCCTGCTCTGTTGGCTGACAAGAATCGGAAATTCATCTACGTTGAAATTGCCTTTTTCCAGCGCTGGTGGAGAGATCAGAGTGATGCCGTCCAGCGTATAGTCACGGACCTTGTCAGCTCGGGTCAACTAGAATTCATAAATGGGGGCATGTGTATGCATGATGAAGCAGCTACGCATTATGTTGACATGATTGATCAGACCACTCTTGGGCATCGCTTTATTAAAACGGAATTCCATGTGACTCCAAGAATTGGATGGCAAATTGATCCTTTTGGACATTCGGCTGTCCAGGCCTACCTGTTGGGAGCACAAGTTGGATTCGACTCACTTTACTTTGGTCGAATAGATTACCAGGACAGGGAAAAACGCAAAAATGACAAGAGTCTTGAGTTTGTTTGGCAGGGTTCTAAGAGTCTCGGTTTATCTGCTGAGATTTTTTCTGGTGCTTTCCCTGAGAATTATGAACCTCCTTCTGGTTTCTATTTTGAAGTTAATGATAAATCCCCAATAGTCCAGGATGACATCACTTTGTTTGATTACAATGTTCAAGAACGAGTTAATGACTTTGTTGCTGCTGCAGTATCACAGGCTAACATAACTCGAACAAATCATATTATGTGGACCATGGGAACAGACTTCAAGTATCAGTATGCACACACCTGGTTCCGTCAAATGGACAAGCTCATTCATTATGTCAACAAGGATGGACGTGTTAATGCTCTGTACTCTACTCCTTCCATATATACTGATGCAAAATATGCTACAAATGAATCTTGGCCAATCAAGACTGGTGACTTCTTTCCTTATGCAGATCGCATTAATGCTTACTGGACAGGATACTTCACAAGCAGACCAGCCTTGAAACACTACGTTAGAACAATGAGTGGCTATTATTTGGCTGCTAGGCAATTAGAATTTTTTAAAGGAAGGAGTACTTCAGGGCCCAATACGGACTCATTGGCAGATGCCTTAGCAATTGCTCAACATCATGATGCTGTCACTGGTACAGAAAAGCAGCACGTTGCCAATGATTATGCAAAAAGATTGTCAATAGGATACATGGAGTCTGAGCAACTAGTTGCAACTTCACTTGCTCACTTGGTTGAGTCTGCATCATATACTGGATCTGGAAACCCAACTACGAAGTTTCAACAGTGTCTACTTCTGAATATAAGCTATTGCCCTGCATCAGAAGTAACTTTGTCTCAGGGGAAAAAATTGATTGTCGTGGCGTACAATTCTCTTGGATGGAAGAGGGATGACACAGTCCGAATTCCTGTTATCAATGAAGATGTCATTGTTCATGATTCTGAGGGAAAAGCAATCGAGTCACAGCTTCTTCCTCTAGATGATGTCCATCTGGGCTTAAGGAACTACTATGTTAAGGCATACTTGGGTCAAACTCCAACAAAGACACCAAAGTATTGGCTTGTGTTTACAGTATCCGTACCACCACTTGGTTTTAGCACATACACTATCTCGGGTGCCGAAGGGGCAGGTGCTAGCTCAACCAGATCATCTGTATACACCTTTCAAAGCAGAGAAAAGTCGACTATTGAAGTTGGTCAAGGAAATGTGAAACTCTCTTTTTCCACAGATCAAGGCAAAATGACTAATTATGTCAATAGCAGAAGCTTGGTGGAAGAATCGGTTCAGCAGTCTTACAGCTTTTATAATGGGTATAATGGCAGTAATGATAAGGCTCCGCTGATTCCTCAGAATTCTGGGGCATATGTTTTCCGGCCTAATGGAACATTTCTCATAACACCCGAAGAAAAGGCTTCTTTGACTGTTATACGTGGACCAGTAATAGATGAAGTACATCAACAGATTAATTCGTGGATACATCAGATCACTAGACTTCACAAGGAAAAGGAGCATGTTGAAGTTGAGTTTATTGTTGGCCCTATACCTACTGATGATGGAATTGGAAAAGAAGTTGTAACTCAATTAGACACTGCCATGGCTACCAACAAAACGTTCTACACTGATTCTAATGGGCGTGATTTTATCAAAAGGATTCGCGACTATAGAACAGATTGGGACTTCAAAGTGAACCAACCTGTTGCTGGAAATTATTACCCAATTAATCTGGGGATTTACATGCAAGATAATAGGACAGAATTTTCAGTTTTGGTGGATCGGTCACTAGGGGGATCCAGCACAGTGGATGGTCAGATAGAGCTGATGCTTCACAGGAGACTACTACTTGATGACTCAAGAGGTGTTGCCGAGGCTCTAAATGAAACTGTCTGTGTTTCCGATGAATGCACAGGACTAAGAGTCCTAGGGAAATTTTACTTCAGAATTGACCCCTTGGGAGATGGTGCTAAGTGGCGTCGTTCTTTTGGCCAGGAGATATATTCACCTCTTCTCCTAGCCTTTGCTGAACAGGATGGAGATAACTGGAAGAATTCTCATGCAACCACTTTTTCTGGGATTGATTCCTCGTATAGTTTACCTGATAATGTTGCTCTTATAACTCTTCAGGAGCTTGATGATGGAAAAATTCTCATTCGCTTAGCACATTTATACGAGGTTGGAGAGGACAAGGATTTTTCTGTAAGGGCAAATGTAGAGCTGAAGAAGCTTTTCCCCAGGAAGAAGATTGGCAAAGCGACAGAAATGAACTTATCAGCTAATCAAGAAAGGACAGAAATGGAGAGAAAGAGACTGGTATGGAAAAAGGAAGGGGGCTCGTCAGGAACGGAAAAGGGTGTGAGGGGTGGACCTGTTGATCCTGCAAAACTAGTTGTGGAACTTGCCCCAATGGAGATCCGCACCTTTCTCATAGACTTGGAGCAGAAGTTCTTCCACCGCCGCCATCATCATCATCATGATGTATCTGATGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0001775 GO:0002252 GO:0002263 GO:0002274 GO:0002275 GO:0002283 GO:0002366 GO:0002376 GO:0002443 GO:0002444 GO:0002446 GO:0003674 GO:0003824 GO:0004553 GO:0004559 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005766 GO:0005773 GO:0005775 GO:0005975 GO:0005996 GO:0006013 GO:0006464 GO:0006517 GO:0006807 GO:0006810 GO:0006887 GO:0006955 GO:0008150 GO:0008152 GO:0009056 GO:0009100 GO:0009311 GO:0009313 GO:0009987 GO:0012505 GO:0015923 GO:0016052 GO:0016192 GO:0016787 GO:0016798 GO:0019318 GO:0019538 GO:0030141 GO:0031410 GO:0031974 GO:0031982 GO:0031983 GO:0032940 GO:0034774 GO:0035578 GO:0036211 GO:0036230 GO:0042119 GO:0042582 GO:0043170 GO:0043202 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043299 GO:0043312 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044281 GO:0044421 GO:0044422 GO:0044424 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045055 GO:0045321 GO:0046903 GO:0050896 GO:0051179 GO:0051234 GO:0060205 GO:0070013 GO:0071704 GO:0097708 GO:0099503 GO:1901135 GO:1901564 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Alpha-mann_mid Glyco_hydro_38 Glyco_hydro_38C

Protein Analysis

1037

Amino Acids

117.05

Weight (kDa)

6.06

Isoelectric Point (pI)

36.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	45 - 356	8e-83	Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid	PF09261	361 - 453	3.4e-21	Alpha mannosidase middle domain
Laman-like_dom	PF21260	528 - 579	5.7e-12	Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C	PF07748	608 - 827	5.9e-47	Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2	PF17677	909 - 1015	9.7e-09	Glycosyl hydrolases family 38 C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001301)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13980 AT5G13980 AT5G13980
fragaria_vesca	FvH4_5g09420 FvH4_5g09421 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272
malus_domestica	MD06G1131600.v1.1 MD09G1002900.v1.1 MD17G1007000.v1.1
prunus_persica	Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.5G142600_v2.0.a1 Prupe.5G142600_v2.0.a1
pyrus_communis	pycom06g12300 pycom06g12310 pycom111g00240 pycom17g00260
rosa_chinensis	RchiOBHm_Chr2g0176471 RchiOBHm_Chr2g0176481 RchiOBHm_Chr7g0188771 RchiOBHm_Chr7g0188781
rosa_laevigata	RLG00000004661 RLG00000004662 RLG00000022416
rosa_multiflora	Rmu_sc0000303.1_g000057 Rmu_sc0003642.1_g000008 Rmu_sc0011904.1_g000012
rosa_roxburghii	Rroxscaffold_2G00076370 Rroxscaffold_3G00266040 Rroxscaffold_3G00266050
rosa_rugosa	Rorug02G0595000 Rorug06G0493700
rosa_samantha	Rh2AG673300 Rh2BG684600 Rh2CG647400 Rh2DG698200 Rh7AG098300 Rh7AG098400 Rh7BG099900 Rh7BG100200 Rh7CG101600 Rh7CG101700 Rh7DG100100 Rh7DG100200
rosa_wichuraiana	Rw0G010390 Rw2G055220 Rw7G008480 Rw7G008490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1932, 2738
AarI	CACCTGC	1 cut(s)	1745
AatII	GACGTC	1 cut(s)	168
Acc36I	ACCTGC	1 cut(s)	1745
AccB1I	GGYRCC	1 cut(s)	1742
AccB7I	CCANNNNNTGG	2 cut(s)	509, 1715
AccI	GTMKAC	3 cut(s)	1417, 1782, 1808
AccII	CGCG	1 cut(s)	2283
AciI	CCGC	4 cut(s)	1964, 3037, 3076, 3079
AclI	AACGTT	1 cut(s)	2237
AclWI	GGATC	7 cut(s)	518, 1390, 2406, 2409, 2422, 2990, 3028
AcoI	YGGCCR	2 cut(s)	1034, 2608
AcuI	CTGAAG	4 cut(s)	1179, 2545, 2729, 2861
AcyI	GRCGYC	2 cut(s)	165, 2596
AfaI	GTAC	6 cut(s)	983, 1192, 1264, 1493, 1707, 2079
AfeI	AGCGCT	1 cut(s)	320
AfiI	CCNNNNNNNGG	9 cut(s)	111, 222, 465, 509, 616, 731, 931, 1715, 2327
AflII	CTTAAG	1 cut(s)	1624
AflIII	ACRYGT	1 cut(s)	967
AhdI	GACNNNNNGTC	1 cut(s)	2540
AhlI	ACTAGT	2 cut(s)	1333, 3007
AjiI	CACGTC	1 cut(s)	968
AjnI	CCWGG	7 cut(s)	219, 546, 609, 771, 917, 2610, 2855
AjuI	GAANNNNNNNTTGG	2 cut(s)	2222, 2254
AloI	GAACNNNNNNTCC	2 cut(s)	3050, 3082
Alw21I	GWGCWC	1 cut(s)	568
Alw26I	GTCTC	4 cut(s)	677, 1467, 2452, 2923
AlwI	GGATC	7 cut(s)	518, 1390, 2406, 2409, 2422, 2990, 3028
Ama87I	CYCGRG	2 cut(s)	376, 1738
Aor51HI	AGCGCT	1 cut(s)	320
AoxI	GGCC	6 cut(s)	549, 1034, 1199, 2000, 2161, 2608
ApaI	GGGCCC	1 cut(s)	1203
ApeKI	GCWGC	5 cut(s)	425, 830, 833, 1157, 1273
ArsI	GACNNNNNNTTYG	4 cut(s)	78, 110, 1289, 1321
AseI	ATTAAT	3 cut(s)	1077, 2091, 2346
Asp700I	GAANNNNTTC	3 cut(s)	1908, 2849, 3068
AspA2I	CCTAGG	1 cut(s)	2545
AspLEI	GCGC	1 cut(s)	321
AspS9I	GGNCC	8 cut(s)	145, 364, 879, 1199, 1200, 2060, 2162, 2987
AsuC2I	CCSGG	1 cut(s)	123
AsuHPI	GGTGA	2 cut(s)	1061, 2616
AsuNHI	GCTAGC	1 cut(s)	1760
AvaI	CYCGRG	2 cut(s)	376, 1738
AvaII	GGWCC	5 cut(s)	145, 364, 879, 2060, 2987
AvrII	CCTAGG	1 cut(s)	2545
BaeGI	GKGCMC	1 cut(s)	1203
BalI	TGGCCA	2 cut(s)	1036, 2610
BamHI	GGATCC	1 cut(s)	2414
BanI	GGYRCC	1 cut(s)	1742
BanII	GRGCYC	2 cut(s)	1203, 2957
BbsI	GAAGAC	1 cut(s)	185
Bbv12I	GWGCWC	1 cut(s)	568
BbvI	GCAGC	5 cut(s)	437, 817, 820, 1144, 1285
BceAI	ACGGC	1 cut(s)	329
BciT130I	CCWGG	7 cut(s)	221, 548, 611, 773, 919, 2612, 2857
BciVI	GTATCC	4 cut(s)	1088, 1307, 1711, 2094
BclI	TGATCA	2 cut(s)	187, 451
BcnI	CCSGG	1 cut(s)	123
BcoDI	GTCTC	4 cut(s)	677, 1467, 2452, 2923
BcuI	ACTAGT	2 cut(s)	1333, 3007
BfmI	CTRYAG	2 cut(s)	834, 2287
BfoI	RGCGCY	1 cut(s)	322
BfrI	CTTAAG	1 cut(s)	1624
BfuAI	ACCTGC	1 cut(s)	1745
BfuI	GTATCC	4 cut(s)	1088, 1307, 1711, 2094
BglI	GCCNNNNNGGC	1 cut(s)	1751
BisI	GCNGC	6 cut(s)	426, 831, 834, 1158, 1274, 3079
BlnI	CCTAGG	1 cut(s)	2545
BlpI	GCTNAGC	1 cut(s)	2779
BlsI	GCNGC	6 cut(s)	427, 832, 835, 1159, 1275, 3080
BmcAI	AGTACT	1 cut(s)	1192
Bme1390I	CCNGG	8 cut(s)	123, 221, 548, 611, 773, 919, 2612, 2857
Bme18I	GGWCC	5 cut(s)	145, 364, 879, 2060, 2987
BmeRI	GACNNNNNGTC	1 cut(s)	2540
BmeT110I	CYCGRG	2 cut(s)	376, 1738
BmgBI	CACGTC	1 cut(s)	968
BmgT120I	GGNCC	8 cut(s)	145, 364, 879, 1199, 1200, 2060, 2162, 2987
BmiI	GGNNCC	7 cut(s)	147, 205, 923, 1201, 1744, 1962, 2416
BmrFI	CCNGG	8 cut(s)	123, 221, 548, 611, 773, 919, 2612, 2857
BmsI	GCATC	8 cut(s)	331, 478, 997, 1213, 1240, 1379, 1454, 2436
BmtI	GCTAGC	1 cut(s)	1764
BoxI	GACNNNNGTC	1 cut(s)	639
BpiI	GAAGAC	1 cut(s)	185
Bpu10I	CCTNAGC	1 cut(s)	1228
Bpu1102I	GCTNAGC	1 cut(s)	2779
BpuEI	CTTGAG	2 cut(s)	665, 2461
BpuMI	CCSGG	1 cut(s)	123
BsaAI	YACGTR	1 cut(s)	2057
BsaBI	GATNNNNATC	1 cut(s)	1614
BsaHI	GRCGYC	2 cut(s)	165, 2596
BsaJI	CCNNGG	9 cut(s)	109, 121, 220, 882, 2220, 2490, 2545, 2574, 2855
BsaXI	ACNNNNNCTCC	7 cut(s)	28, 555, 585, 2616, 2646, 3050, 3080
Bsc4I	CCNNNNNNNGG	9 cut(s)	111, 222, 465, 509, 616, 731, 931, 1715, 2327
Bse1I	ACTGG	7 cut(s)	1051, 1091, 1264, 1384, 2063, 2669, 2937
Bse8I	GATNNNNATC	1 cut(s)	1614
BseBI	CCWGG	7 cut(s)	221, 548, 611, 773, 919, 2612, 2857
BseDI	CCNNGG	9 cut(s)	109, 121, 220, 882, 2220, 2490, 2545, 2574, 2855
BseGI	GGATG	9 cut(s)	176, 216, 518, 781, 967, 1511, 1522, 2434, 2659
BseJI	GATNNNNATC	1 cut(s)	1614
BseLI	CCNNNNNNNGG	9 cut(s)	111, 222, 465, 509, 616, 731, 931, 1715, 2327
BseMII	CTCAG	6 cut(s)	678, 702, 1317, 1478, 1560, 1988
BseNI	ACTGG	7 cut(s)	1051, 1091, 1264, 1384, 2063, 2669, 2937
BseRI	GAGGAG	1 cut(s)	6
BseSI	GKGCMC	1 cut(s)	1203
BseXI	GCAGC	5 cut(s)	437, 817, 820, 1144, 1285
Bsh1236I	CGCG	1 cut(s)	2283
Bsh1285I	CGRYCG	1 cut(s)	60
BshFI	GGCC	6 cut(s)	551, 1036, 1201, 2002, 2163, 2610
BshNI	GGYRCC	1 cut(s)	1742
BsiEI	CGRYCG	1 cut(s)	60
BsiHKAI	GWGCWC	1 cut(s)	568
BsiHKCI	CYCGRG	2 cut(s)	376, 1738
BsiSI	CCGG	2 cut(s)	123, 1999
BslFI	GGGAC	3 cut(s)	104, 131, 2315
BslI	CCNNNNNNNGG	9 cut(s)	111, 222, 465, 509, 616, 731, 931, 1715, 2327
BsmAI	GTCTC	4 cut(s)	677, 1467, 2452, 2923
BsmFI	GGGAC	3 cut(s)	104, 131, 2315
BsmI	GAATGC	1 cut(s)	2531
BsnI	GGCC	6 cut(s)	551, 1036, 1201, 2002, 2163, 2610
BsoBI	CYCGRG	2 cut(s)	376, 1738
Bsp120I	GGGCCC	1 cut(s)	1199
Bsp1286I	GDGCHC	3 cut(s)	568, 1203, 2957
Bsp1720I	GCTNAGC	1 cut(s)	2779
Bsp19I	CCATGG	2 cut(s)	882, 2220
Bsp68I	TCGCGA	1 cut(s)	2283
BspACI	CCGC	4 cut(s)	1964, 3037, 3076, 3079
BspANI	GGCC	6 cut(s)	551, 1036, 1201, 2002, 2163, 2610
BspCNI	CTCAG	6 cut(s)	679, 703, 1318, 1477, 1561, 1987
BspFNI	CGCG	1 cut(s)	2283
BspHI	TCATGA	3 cut(s)	1246, 1561, 3094
BspLI	GGNNCC	7 cut(s)	147, 205, 923, 1201, 1744, 1962, 2416
BspMAI	CTGCAG	1 cut(s)	838
BspMI	ACCTGC	1 cut(s)	1745
BspOI	GCTAGC	1 cut(s)	1764
BspPI	GGATC	7 cut(s)	518, 1390, 2406, 2409, 2422, 2990, 3028
BspT107I	GGYRCC	1 cut(s)	1742
BspTI	CTTAAG	1 cut(s)	1624
BsrI	ACTGG	7 cut(s)	1051, 1091, 1264, 1384, 2063, 2669, 2937
BssECI	CCNNGG	9 cut(s)	109, 121, 220, 882, 2220, 2490, 2545, 2574, 2855
BssNAI	GTATAC	1 cut(s)	1783
BssNI	GRCGYC	2 cut(s)	165, 2596
BssT1I	CCWWGG	5 cut(s)	109, 882, 2220, 2545, 2574
Bst1107I	GTATAC	1 cut(s)	1783
Bst2UI	CCWGG	7 cut(s)	221, 548, 611, 773, 919, 2612, 2857
Bst4CI	ACNGT	7 cut(s)	184, 1413, 1525, 1699, 2050, 2425, 2509
Bst6I	CTCTTC	4 cut(s)	32, 1505, 2634, 2748
BstACI	GRCGYC	2 cut(s)	165, 2596
BstAFI	CTTAAG	1 cut(s)	1624
BstAPI	GCANNNNNTGC	1 cut(s)	1016
BstBAI	YACGTR	1 cut(s)	2057
BstC8I	GCNNGC	1 cut(s)	1762
BstDSI	CCRYGG	2 cut(s)	882, 2220
BstF5I	GGATG	9 cut(s)	176, 216, 518, 781, 967, 1511, 1522, 2434, 2659
BstFNI	CGCG	1 cut(s)	2283
BstH2I	RGCGCY	1 cut(s)	322
BstHHI	GCGC	1 cut(s)	321
BstMAI	GTCTC	4 cut(s)	677, 1467, 2452, 2923
BstMCI	CGRYCG	1 cut(s)	60
BstMWI	GCNNNNNNNGC	5 cut(s)	269, 548, 1016, 1751, 2845
BstNI	CCWGG	7 cut(s)	221, 548, 611, 773, 919, 2612, 2857
BstNSI	RCATGY	3 cut(s)	409, 2141, 2365
BstPAI	GACNNNNGTC	1 cut(s)	639
BstSCI	CCNGG	8 cut(s)	121, 219, 546, 609, 771, 917, 2610, 2855
BstSFI	CTRYAG	2 cut(s)	834, 2287
BstSLI	GKGCMC	1 cut(s)	1203
BstUI	CGCG	1 cut(s)	2283
BstV1I	GCAGC	5 cut(s)	437, 817, 820, 1144, 1285
BstV2I	GAAGAC	1 cut(s)	185
BstX2I	RGATCY	3 cut(s)	1382, 2414, 3033
BstXI	CCANNNNNNTGG	3 cut(s)	91, 1683, 2425
BstYI	RGATCY	3 cut(s)	1382, 2414, 3033
BstZ17I	GTATAC	1 cut(s)	1783
BsuI	GTATCC	4 cut(s)	1088, 1307, 1711, 2094
BsuRI	GGCC	6 cut(s)	551, 1036, 1201, 2002, 2163, 2610
BtgI	CCRYGG	2 cut(s)	882, 2220
BtgZI	GCGATG	1 cut(s)	455
BtrI	CACGTC	1 cut(s)	968
BtsCI	GGATG	9 cut(s)	176, 216, 518, 781, 967, 1511, 1522, 2434, 2659
BtsI	GCAGTG	1 cut(s)	2214
BtsIMutI	CAGTG	5 cut(s)	1257, 1418, 2214, 2244, 2430
BtuMI	TCGCGA	1 cut(s)	2283
BveI	ACCTGC	1 cut(s)	1745
Cac8I	GCNNGC	1 cut(s)	1762
CciI	TCATGA	3 cut(s)	1246, 1561, 3094
CfoI	GCGC	1 cut(s)	321
Cfr13I	GGNCC	8 cut(s)	145, 364, 879, 1199, 1200, 2060, 2162, 2987
CseI	GACGC	1 cut(s)	2585
CsiI	ACCWGGT	1 cut(s)	917
Csp6I	GTAC	6 cut(s)	982, 1191, 1263, 1492, 1706, 2078
CviQI	GTAC	6 cut(s)	982, 1191, 1263, 1492, 1706, 2078
DraI	TTTAAA	1 cut(s)	1180
DriI	GACNNNNNGTC	1 cut(s)	2540
EaeI	YGGCCR	2 cut(s)	1034, 2608
Eam1104I	CTCTTC	4 cut(s)	32, 1505, 2634, 2748
Eam1105I	GACNNNNNGTC	1 cut(s)	2540
EarI	CTCTTC	4 cut(s)	32, 1505, 2634, 2748
Eco130I	CCWWGG	5 cut(s)	109, 882, 2220, 2545, 2574
Eco147I	AGGCCT	1 cut(s)	551
Eco24I	GRGCYC	2 cut(s)	1203, 2957
Eco47I	GGWCC	5 cut(s)	145, 364, 879, 2060, 2987
Eco47III	AGCGCT	1 cut(s)	320
Eco57I	CTGAAG	4 cut(s)	1179, 2545, 2729, 2861
Eco88I	CYCGRG	2 cut(s)	376, 1738
EcoO109I	RGGNCCY	1 cut(s)	1199
EcoRI	GAATTC	5 cut(s)	389, 488, 1530, 1978, 2671
EcoRII	CCWGG	7 cut(s)	219, 546, 609, 771, 917, 2610, 2855
EcoT14I	CCWWGG	5 cut(s)	109, 882, 2220, 2545, 2574
EcoT22I	ATGCAT	1 cut(s)	417
EcoT38I	GRGCYC	2 cut(s)	1203, 2957
ErhI	CCWWGG	5 cut(s)	109, 882, 2220, 2545, 2574
FalI	AAGNNNNNCTT	4 cut(s)	1177, 1209, 1634, 1666
FaqI	GGGAC	3 cut(s)	104, 131, 2315
FauNDI	CATATG	1 cut(s)	1990
FbaI	TGATCA	2 cut(s)	187, 451
FblI	GTMKAC	3 cut(s)	1417, 1782, 1808
Fnu4HI	GCNGC	6 cut(s)	426, 831, 834, 1158, 1274, 3079
FokI	GGATG	9 cut(s)	183, 203, 525, 788, 974, 1518, 1529, 2441, 2666
FriOI	GRGCYC	2 cut(s)	1203, 2957
Fsp4HI	GCNGC	6 cut(s)	426, 831, 834, 1158, 1274, 3079
GlaI	GCGC	1 cut(s)	320
GluI	GCNGC	6 cut(s)	426, 831, 834, 1158, 1274, 3079
HaeII	RGCGCY	1 cut(s)	322
HaeIII	GGCC	6 cut(s)	551, 1036, 1201, 2002, 2163, 2610
HapII	CCGG	2 cut(s)	123, 1999
HgaI	GACGC	1 cut(s)	2585
HhaI	GCGC	1 cut(s)	321
Hin1I	GRCGYC	2 cut(s)	165, 2596
Hin6I	GCGC	1 cut(s)	319
HinP1I	GCGC	1 cut(s)	319
HincII	GTYRAC	4 cut(s)	383, 442, 955, 1809
HindII	GTYRAC	4 cut(s)	383, 442, 955, 1809
HindIII	AAGCTT	2 cut(s)	1891, 2846
HpaII	CCGG	2 cut(s)	123, 1999
HphI	GGTGA	2 cut(s)	1061, 2616
Hpy99I	CGWCG	4 cut(s)	164, 167, 170, 2601
HpyAV	CCTTC	5 cut(s)	738, 1002, 1179, 1742, 2942
HpyCH4III	ACNGT	7 cut(s)	184, 1413, 1525, 1699, 2050, 2425, 2509
HpyCH4IV	ACGT	8 cut(s)	135, 165, 297, 967, 1128, 1278, 2056, 2237
HpyF10VI	GCNNNNNNNGC	5 cut(s)	269, 548, 1016, 1751, 2845
HpySE526I	ACGT	8 cut(s)	135, 165, 297, 967, 1128, 1278, 2056, 2237
Hsp92I	GRCGYC	2 cut(s)	165, 2596
HspAI	GCGC	1 cut(s)	319
Ksp22I	TGATCA	2 cut(s)	187, 451
LmnI	GCTCC	6 cut(s)	209, 563, 1966, 2134, 2749, 3058
Lsp1109I	GCAGC	5 cut(s)	437, 817, 820, 1144, 1285
LweI	GCATC	8 cut(s)	331, 478, 997, 1213, 1240, 1379, 1454, 2436
MabI	ACCWGGT	1 cut(s)	917
MaeII	ACGT	8 cut(s)	135, 165, 297, 967, 1128, 1278, 2056, 2237
MaeIII	GTNAC	8 cut(s)	358, 496, 1049, 1255, 1453, 1587, 2200, 2403
MfeI	CAATTG	1 cut(s)	1233
MflI	RGATCY	3 cut(s)	1382, 2414, 3033
MhlI	GDGCHC	3 cut(s)	568, 1203, 2957
MlsI	TGGCCA	2 cut(s)	1036, 2610
MluNI	TGGCCA	2 cut(s)	1036, 2610
MmeI	TCCRAC	6 cut(s)	148, 225, 231, 553, 1688, 2779
Mox20I	TGGCCA	2 cut(s)	1036, 2610
Mph1103I	ATGCAT	1 cut(s)	417
MroXI	GAANNNNTTC	3 cut(s)	1908, 2849, 3068
MscI	TGGCCA	2 cut(s)	1036, 2610
MslI	CAYNNNNRTG	5 cut(s)	410, 1248, 2219, 3093, 3096
Msp20I	TGGCCA	2 cut(s)	1036, 2610
MspA1I	CMGCKG	1 cut(s)	1966
MspCI	CTTAAG	1 cut(s)	1624
MspI	CCGG	2 cut(s)	123, 1999
MspR9I	CCNGG	8 cut(s)	123, 221, 548, 611, 773, 919, 2612, 2857
MunI	CAATTG	1 cut(s)	1233
Mva1269I	GAATGC	1 cut(s)	2531
MvaI	CCWGG	7 cut(s)	221, 548, 611, 773, 919, 2612, 2857
MvnI	CGCG	1 cut(s)	2283
MwoI	GCNNNNNNNGC	5 cut(s)	269, 548, 1016, 1751, 2845
NciI	CCSGG	1 cut(s)	123
NcoI	CCATGG	2 cut(s)	882, 2220
NdeI	CATATG	1 cut(s)	1990
NheI	GCTAGC	1 cut(s)	1760
NlaIV	GGNNCC	7 cut(s)	147, 205, 923, 1201, 1744, 1962, 2416
NmeAIII	GCCGAG	1 cut(s)	2515
NmuCI	GTSAC	6 cut(s)	358, 496, 1049, 1255, 1587, 2403
NruI	TCGCGA	1 cut(s)	2283
NsiI	ATGCAT	1 cut(s)	417
NspI	RCATGY	3 cut(s)	409, 2141, 2365
PagI	TCATGA	3 cut(s)	1246, 1561, 3094
PaqCI	CACCTGC	1 cut(s)	1745
PceI	AGGCCT	1 cut(s)	551
PctI	GAATGC	1 cut(s)	2531
PdmI	GAANNNNTTC	3 cut(s)	1908, 2849, 3068
PflFI	GACNNNGTC	3 cut(s)	164, 368, 1523
PflMI	CCANNNNNTGG	2 cut(s)	509, 1715
PfoI	TCCNGGA	1 cut(s)	771
PkrI	GCNGC	6 cut(s)	427, 832, 835, 1159, 1275, 3080
Ppu21I	YACGTR	1 cut(s)	2057
PshAI	GACNNNNGTC	1 cut(s)	639
PshBI	ATTAAT	3 cut(s)	1077, 2091, 2346
PsiI	TTATAA	2 cut(s)	1932, 2738
Psp1406I	AACGTT	1 cut(s)	2237
Psp6I	CCWGG	7 cut(s)	219, 546, 609, 771, 917, 2610, 2855
PspGI	CCWGG	7 cut(s)	219, 546, 609, 771, 917, 2610, 2855
PspN4I	GGNNCC	7 cut(s)	147, 205, 923, 1201, 1744, 1962, 2416
PspOMI	GGGCCC	1 cut(s)	1199
PspPI	GGNCC	8 cut(s)	145, 364, 879, 1199, 1200, 2060, 2162, 2987
PstI	CTGCAG	1 cut(s)	838
PsuI	RGATCY	3 cut(s)	1382, 2414, 3033
PsyI	GACNNNGTC	3 cut(s)	164, 368, 1523
RruI	TCGCGA	1 cut(s)	2283
RsaI	GTAC	6 cut(s)	983, 1192, 1264, 1493, 1707, 2079
RsaNI	GTAC	6 cut(s)	982, 1191, 1263, 1492, 1706, 2078
RseI	CAYNNNNRTG	5 cut(s)	410, 1248, 2219, 3093, 3096
SalI	GTCGAC	1 cut(s)	1807
SatI	GCNGC	6 cut(s)	426, 831, 834, 1158, 1274, 3079
Sau96I	GGNCC	8 cut(s)	145, 364, 879, 1199, 1200, 2060, 2162, 2987
ScaI	AGTACT	1 cut(s)	1192
ScrFI	CCNGG	8 cut(s)	123, 221, 548, 611, 773, 919, 2612, 2857
SduI	GDGCHC	3 cut(s)	568, 1203, 2957
SexAI	ACCWGGT	1 cut(s)	917
SfaNI	GCATC	8 cut(s)	331, 478, 997, 1213, 1240, 1379, 1454, 2436
SfcI	CTRYAG	2 cut(s)	834, 2287
SinI	GGWCC	5 cut(s)	145, 364, 879, 2060, 2987
SmiMI	CAYNNNNRTG	5 cut(s)	410, 1248, 2219, 3093, 3096
SmlI	CTYRAG	3 cut(s)	644, 1624, 2476
SmoI	CTYRAG	3 cut(s)	644, 1624, 2476
SpeI	ACTAGT	2 cut(s)	1333, 3007
SseBI	AGGCCT	1 cut(s)	551
SsiI	CCGC	4 cut(s)	1964, 3037, 3076, 3079
StuI	AGGCCT	1 cut(s)	551
StyD4I	CCNGG	8 cut(s)	121, 219, 546, 609, 771, 917, 2610, 2855
StyI	CCWWGG	5 cut(s)	109, 882, 2220, 2545, 2574
TaaI	ACNGT	7 cut(s)	184, 1413, 1525, 1699, 2050, 2425, 2509
TaiI	ACGT	8 cut(s)	138, 168, 300, 970, 1131, 1281, 2059, 2240
TaqI	TCGA	5 cut(s)	579, 598, 859, 1584, 1808
TaqII	GACCGA	2 cut(s)	46, 2391
TatI	WGTACW	3 cut(s)	981, 1190, 2077
TauI	GCSGC	1 cut(s)	3081
TscAI	CASTG	5 cut(s)	1264, 1418, 2221, 2251, 2430
TseFI	GTSAC	6 cut(s)	358, 496, 1049, 1255, 1587, 2403
TseI	GCWGC	5 cut(s)	425, 830, 833, 1157, 1273
Tsp45I	GTSAC	6 cut(s)	358, 496, 1049, 1255, 1587, 2403
TspGWI	ACGGA	6 cut(s)	377, 500, 914, 1223, 1693, 2981
TspRI	CASTG	5 cut(s)	1264, 1418, 2221, 2251, 2430
Tth111I	GACNNNGTC	3 cut(s)	164, 368, 1523
Van91I	CCANNNNNTGG	2 cut(s)	509, 1715
Vha464I	CTTAAG	1 cut(s)	1624
VpaK11BI	GGWCC	5 cut(s)	145, 364, 879, 2060, 2987
VspI	ATTAAT	3 cut(s)	1077, 2091, 2346
XbaI	TCTAGA	1 cut(s)	1603
XceI	RCATGY	3 cut(s)	409, 2141, 2365
XcmI	CCANNNNNNNNNTGG	2 cut(s)	510, 2324
XmaJI	CCTAGG	1 cut(s)	2545
XmiI	GTMKAC	3 cut(s)	1417, 1782, 1808
XmnI	GAANNNNTTC	3 cut(s)	1908, 2849, 3068
ZraI	GACGTC	1 cut(s)	166
ZrmI	AGTACT	1 cut(s)	1192
Zsp2I	ATGCAT	1 cut(s)	417

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.