Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0001301

Physical Location & Seq

Genomic Coordinates Reverse (-)

82682196 .. 82690479

Gene Structure 8284 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G055220.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3108 bp

ATGGCGACCACGACTCCTCGTTTACTCTTCTTCTTCTTCTGTGTGCTTTTCTCGGTCGGGGGGATTCTAGTCGCTGACTCCAAATTTATGGTCTATAACACCTCCCAAGGGATAGTCGCCGATAAACTTAACGTCCACTTGGTCCCTCACACCCACGACGACGTCGGATGGTTGAAGACCGTTGATCAGTATTATGTTGGCTCCAACAATTCCATCCAGGGGGCTTGTGTGCAAAATGTGTTGGACTCTCTGATTCCTGCTCTGTTGGCTGACAAGAATCGGAAATTCATCTACGTTGAAATTGCCTTTTTCCAGCGCTGGTGGAGAGATCAGAGTGATGCCGTCCAGCGTATAGTCACGGACCTTGTCCGCTCGGGTCAACTAGAATTCATAAATGGGGGCATGTGTATGCATGATGAAGCAGCTACGCATTATGTTGACATGATTGATCAGACCACTCTTGGGCATCGCTTTATTAAAACGGAATTCGATGTGACTCCAAGAATTGGATGGCAAATTGATCCTTTTGGACATTCGGCTGTCCAGGCCTACTTGTTGGGAGCACAAGTTGGATTCGACTCACTTTACTTTGGTCGAATAGATTACCAGGACAGGGAAAAACGCAAAAATGACAAGAGTCTGGAGTTTGTTTGGCAGGGTTCTAAGAGTCTCGGTTCATCTGCTGAGATTTTTTCTGGTGCTTTCCCTGAGAATTATGAACCTCCTTCTGGTTTCTATTTTGAAGTTAATGATAAATCCCCAATAGTCCAGGATGACATCACTTTGTTTGATTACAATGTTCAAGAACGAGTTAATGACTTTGTTGCTGCTGCAGTATCACAGGCTAACATAACTCGAACAAATCATATTATGTGGACCATGGGAACAGACTTCAAGTATCAGTATGCACACACCTGGTTCCGTCAAATGGACAAGCTCATTCATTATGTCAACAAGGATGGACGTGTTAATGCTCTGTACTCTACTCCTTCCATATATACTGATGCAAAATATGCTACAAATGAATCTTGGCCAATCAAGACTGGTGACTTCTTTCCTTATGCAGATCGCATTAATGCTTACTGGACAGGATACTTCACAAGCAGACCAGCCTTGAAACACTACGTTAGAACAATGAGTGGCTATTATTTGGCTGCTAGGCAACTAGAATTTTTTAAAGGAAGGAGTACTTCAGGGCCCAATACGGACTCACTGGCAGATGCCTTAGCAATTGCTCAACATCATGATGCTGTCACTGGTACAGAAAAGCAGCACGTTGCCAATGATTATGCAAAAAGATTGTCAATAGGATACATGGAGTCTGAGCAACTAGTTGCAACTTCACTTGCTCACTTGGTTGAGTCTGCATCATATACTGGATCTGGAAACCCAACTACAAAGTTTCAACAGTGTCTACTTCTGAATATAAGCTATTGCCCTGCATCAGAAGTAACTTTGTCTCAGGGGAAAAAATTGATTGTCGTGGTGTACAATTCTCTTGGATGGAAGAGGGATGACACAGTCCGAATTCCTGTTATCAATGAAGATGTCATTGTTCATGATTCTGAGGGAAAAGCAATCGAGTCACAGCTTCTTCCTCTAGATGATGTCCATCTGGGCTTAAGGAACTACTATGTTAAGGCATACTTGGGTCAAACTCCAACAAAGACACCAAAGTATTGGCTTGTGTTTACAGTATCCGTACCACCACTTGGTTTTAGCACATACACTATCTCGGGTGCCGAAGGGGCAGGTGCTAGCTCAACCAGATCATCTGTATACACCTTTCAAAGCAGAGAAAAGTCGACTATTGAAGTTGGTCAAGGAAATGTGAAACTCTCTTTTTCCACAGATCAAGGCAAAATGACTAATTATGTCAATAGCAGAAGCTTGGTGGAAGAATCGGTTCAGCAGTCTTACAGCTTTTATAATGGGTATAATGGCAGTAATGATAAGGCTCCGCTGATTCCTCAGAATTCTGGGGCATATGTTTTCCGGCCTAATGGAACATTTCTCATAACACCCGAAGAAAAGGCTTCTTTGACTGTTATACGTGGACCAGTAATAGATGAAGTACATCAACAGATTAATTCGTGGATACATCAGATCACTAGACTTCACAAGGAAAAGGAGCATGTTGAAGTTGAGTTTATTGTTGGCCCTATACCTACTGATGATGGAATTGGAAAAGAAGTTGTAACTCAATTAGACACTGCCATGGCTACCAACAAAACGTTCTACACTGATTCTAATGGGCGTGATTTTATCAAAAGGATTCGCGACTATAGAACAGATTGGGACTTCAAAGTGAACCAACCTGTTGCTGGAAATTATTACCCAATTAATCTTGGGATTTACATGCAAGATAATAGGACAGAATTTTCAGTTTTGGTGGATCGGTCACTAGGGGGATCCAGCACAGTGGATGGTCAGATAGAACTGATGCTTCACAGGAGACTACTACTTGATGACTCAAGAGGTGTTGCCGAGGCTCTAAATGAAACTGTCTGTGTTTCCGATGAATGCACAGGACTAAGAGTCCTAGGGAAATTTTACTTCAGAATTGACCCCTTGGGAGATGGTGCTAAGTGGCGTCGTTCTTTTGGCCAGGAGATATATTCACCTCTTCTCCTAGCCTTTGCTGAACAGGATGGAGATAACTGGAAGAATTCTCATGCAACCACTTTTTCTGGGATTGATTCCTCGTATAGTTTACCTGATAATGTTGCTCTTATAACTCTTCAGGAGCTTGATGATGGAAAAATTCTCATTCGCTTAGCACATTTATACGAGGTTGGAGAGGACAAGGATTTTTCTGTAATGGCAAATGTAGAGCTGAAGAAGCTTTTCCCCAGGAAGAAGATTGGCAAAGCGACAGAAATGAACTTATCAGCTAATCAAGAAAGGACAGAAATGGAGAGAAAGAGACTGGTATGGAAAAAGGAAGGGGGCTCGTCAGGAACGGAAAAGGGTGTGAGGGGTGGACCTGTTGATCCTGCAAAACTAGTTGTGGAACTTGCCCCAATGGAAATCCGCACCTTTCTCATTGACTTGGAGCAGAAGTTCTTCCACCGCCGCCATCATCATGATGATGTATCTGATGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0001775 GO:0002252 GO:0002263 GO:0002274 GO:0002275 GO:0002283 GO:0002366 GO:0002376 GO:0002443 GO:0002444 GO:0002446 GO:0003674 GO:0003824 GO:0004553 GO:0004559 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005766 GO:0005773 GO:0005775 GO:0005975 GO:0005996 GO:0006013 GO:0006464 GO:0006517 GO:0006807 GO:0006810 GO:0006887 GO:0006955 GO:0008150 GO:0008152 GO:0009056 GO:0009100 GO:0009311 GO:0009313 GO:0009987 GO:0012505 GO:0015923 GO:0016052 GO:0016192 GO:0016787 GO:0016798 GO:0019318 GO:0019538 GO:0030141 GO:0031410 GO:0031974 GO:0031982 GO:0031983 GO:0032940 GO:0034774 GO:0035578 GO:0036211 GO:0036230 GO:0042119 GO:0042582 GO:0043170 GO:0043202 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043299 GO:0043312 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044281 GO:0044421 GO:0044422 GO:0044424 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045055 GO:0045321 GO:0046903 GO:0050896 GO:0051179 GO:0051234 GO:0060205 GO:0070013 GO:0071704 GO:0097708 GO:0099503 GO:1901135 GO:1901564 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Alpha-mann_mid Glyco_hydro_38 Glyco_hydro_38C

Protein Analysis

1035

Amino Acids

116.84

Weight (kDa)

5.86

Isoelectric Point (pI)

36.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	44 - 355	5.9e-83	Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid	PF09261	360 - 452	3.3e-21	Alpha mannosidase middle domain
Laman-like_dom	PF21260	527 - 578	5.7e-12	Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C	PF07748	607 - 826	5.9e-47	Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2	PF17677	908 - 1014	1e-08	Glycosyl hydrolases family 38 C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001301)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13980 AT5G13980 AT5G13980
fragaria_vesca	FvH4_5g09420 FvH4_5g09421 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272
malus_domestica	MD06G1131600.v1.1 MD09G1002900.v1.1 MD17G1007000.v1.1
prunus_persica	Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.5G142600_v2.0.a1 Prupe.5G142600_v2.0.a1
pyrus_communis	pycom06g12300 pycom06g12310 pycom111g00240 pycom17g00260
rosa_chinensis	RchiOBHm_Chr2g0176471 RchiOBHm_Chr2g0176481 RchiOBHm_Chr7g0188771 RchiOBHm_Chr7g0188781
rosa_laevigata	RLG00000004661 RLG00000004662 RLG00000022416
rosa_multiflora	Rmu_sc0000303.1_g000057 Rmu_sc0003642.1_g000008 Rmu_sc0011904.1_g000012
rosa_roxburghii	Rroxscaffold_2G00076370 Rroxscaffold_3G00266040 Rroxscaffold_3G00266050
rosa_rugosa	Rorug02G0595000 Rorug06G0493700
rosa_samantha	Rh2AG673300 Rh2BG684600 Rh2CG647400 Rh2DG698200 Rh7AG098300 Rh7AG098400 Rh7BG099900 Rh7BG100200 Rh7CG101600 Rh7CG101700 Rh7DG100100 Rh7DG100200
rosa_wichuraiana	Rw0G010390 Rw2G055220 Rw7G008480 Rw7G008490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1929, 2735
AarI	CACCTGC	1 cut(s)	1742
AatII	GACGTC	1 cut(s)	165
Acc36I	ACCTGC	1 cut(s)	1742
AccB1I	GGYRCC	1 cut(s)	1739
AccB7I	CCANNNNNTGG	2 cut(s)	506, 1712
AccBSI	CCGCTC	1 cut(s)	372
AccI	GTMKAC	3 cut(s)	1414, 1779, 1805
AccII	CGCG	1 cut(s)	2280
AciI	CCGC	5 cut(s)	370, 1961, 3034, 3073, 3076
AclI	AACGTT	1 cut(s)	2234
AclWI	GGATC	6 cut(s)	515, 1387, 2403, 2406, 2419, 2987
AcoI	YGGCCR	2 cut(s)	1031, 2605
AcuI	CTGAAG	4 cut(s)	1176, 2542, 2726, 2858
AcyI	GRCGYC	2 cut(s)	162, 2593
AfaI	GTAC	6 cut(s)	980, 1189, 1261, 1490, 1704, 2076
AfeI	AGCGCT	1 cut(s)	317
AfiI	CCNNNNNNNGG	9 cut(s)	108, 219, 462, 506, 613, 728, 928, 1712, 2324
AflII	CTTAAG	1 cut(s)	1621
AflIII	ACRYGT	1 cut(s)	964
AhdI	GACNNNNNGTC	1 cut(s)	2537
AhlI	ACTAGT	2 cut(s)	1330, 3004
AjiI	CACGTC	1 cut(s)	965
AjnI	CCWGG	7 cut(s)	216, 543, 606, 768, 914, 2607, 2852
AjuI	GAANNNNNNNTTGG	2 cut(s)	2219, 2251
AloI	GAACNNNNNNTCC	2 cut(s)	3047, 3079
Alw21I	GWGCWC	1 cut(s)	565
Alw26I	GTCTC	4 cut(s)	674, 1464, 2449, 2920
AlwI	GGATC	6 cut(s)	515, 1387, 2403, 2406, 2419, 2987
Ama87I	CYCGRG	2 cut(s)	373, 1735
Aor51HI	AGCGCT	1 cut(s)	317
AoxI	GGCC	6 cut(s)	546, 1031, 1196, 1997, 2158, 2605
ApaI	GGGCCC	1 cut(s)	1200
ApeKI	GCWGC	5 cut(s)	422, 827, 830, 1154, 1270
ArsI	GACNNNNNNTTYG	4 cut(s)	75, 107, 1286, 1318
AseI	ATTAAT	3 cut(s)	1074, 2088, 2343
Asp700I	GAANNNNTTC	3 cut(s)	1905, 2846, 3065
AspA2I	CCTAGG	1 cut(s)	2542
AspLEI	GCGC	1 cut(s)	318
AspS9I	GGNCC	8 cut(s)	142, 361, 876, 1196, 1197, 2057, 2159, 2984
AsuHPI	GGTGA	2 cut(s)	1058, 2613
AsuNHI	GCTAGC	1 cut(s)	1757
AvaI	CYCGRG	2 cut(s)	373, 1735
AvaII	GGWCC	5 cut(s)	142, 361, 876, 2057, 2984
AvrII	CCTAGG	1 cut(s)	2542
BaeGI	GKGCMC	1 cut(s)	1200
BalI	TGGCCA	2 cut(s)	1033, 2607
BamHI	GGATCC	1 cut(s)	2411
BanI	GGYRCC	1 cut(s)	1739
BanII	GRGCYC	2 cut(s)	1200, 2954
BbsI	GAAGAC	1 cut(s)	182
Bbv12I	GWGCWC	1 cut(s)	565
BbvI	GCAGC	5 cut(s)	434, 814, 817, 1141, 1282
BceAI	ACGGC	1 cut(s)	326
BciT130I	CCWGG	7 cut(s)	218, 545, 608, 770, 916, 2609, 2854
BciVI	GTATCC	4 cut(s)	1085, 1304, 1708, 2091
BclI	TGATCA	2 cut(s)	184, 448
BcoDI	GTCTC	4 cut(s)	674, 1464, 2449, 2920
BcuI	ACTAGT	2 cut(s)	1330, 3004
BfmI	CTRYAG	2 cut(s)	831, 2284
BfoI	RGCGCY	1 cut(s)	319
BfrI	CTTAAG	1 cut(s)	1621
BfuAI	ACCTGC	1 cut(s)	1742
BfuI	GTATCC	4 cut(s)	1085, 1304, 1708, 2091
BglI	GCCNNNNNGGC	1 cut(s)	1748
BisI	GCNGC	6 cut(s)	423, 828, 831, 1155, 1271, 3076
BlnI	CCTAGG	1 cut(s)	2542
BlpI	GCTNAGC	1 cut(s)	2776
BlsI	GCNGC	6 cut(s)	424, 829, 832, 1156, 1272, 3077
BmcAI	AGTACT	1 cut(s)	1189
Bme1390I	CCNGG	7 cut(s)	218, 545, 608, 770, 916, 2609, 2854
Bme18I	GGWCC	5 cut(s)	142, 361, 876, 2057, 2984
BmeRI	GACNNNNNGTC	1 cut(s)	2537
BmeT110I	CYCGRG	2 cut(s)	373, 1735
BmgBI	CACGTC	1 cut(s)	965
BmgT120I	GGNCC	8 cut(s)	142, 361, 876, 1196, 1197, 2057, 2159, 2984
BmiI	GGNNCC	7 cut(s)	144, 202, 920, 1198, 1741, 1959, 2413
BmrFI	CCNGG	7 cut(s)	218, 545, 608, 770, 916, 2609, 2854
BmsI	GCATC	8 cut(s)	328, 475, 994, 1210, 1237, 1376, 1451, 2433
BmtI	GCTAGC	1 cut(s)	1761
BoxI	GACNNNNGTC	1 cut(s)	636
BpiI	GAAGAC	1 cut(s)	182
BpmI	CTGGAG	1 cut(s)	662
Bpu10I	CCTNAGC	1 cut(s)	1225
Bpu1102I	GCTNAGC	1 cut(s)	2776
BpuEI	CTTGAG	1 cut(s)	2458
BsaAI	YACGTR	1 cut(s)	2054
BsaBI	GATNNNNATC	1 cut(s)	1611
BsaHI	GRCGYC	2 cut(s)	162, 2593
BsaJI	CCNNGG	8 cut(s)	106, 217, 879, 2217, 2487, 2542, 2571, 2852
BsaXI	ACNNNNNCTCC	7 cut(s)	28, 552, 582, 2613, 2643, 3047, 3077
Bsc4I	CCNNNNNNNGG	9 cut(s)	108, 219, 462, 506, 613, 728, 928, 1712, 2324
Bse1I	ACTGG	8 cut(s)	1048, 1088, 1218, 1261, 1381, 2060, 2666, 2934
Bse8I	GATNNNNATC	1 cut(s)	1611
BseBI	CCWGG	7 cut(s)	218, 545, 608, 770, 916, 2609, 2854
BseDI	CCNNGG	8 cut(s)	106, 217, 879, 2217, 2487, 2542, 2571, 2852
BseGI	GGATG	9 cut(s)	173, 213, 515, 778, 964, 1508, 1519, 2431, 2656
BseJI	GATNNNNATC	1 cut(s)	1611
BseLI	CCNNNNNNNGG	9 cut(s)	108, 219, 462, 506, 613, 728, 928, 1712, 2324
BseMII	CTCAG	6 cut(s)	675, 699, 1314, 1475, 1557, 1985
BseNI	ACTGG	8 cut(s)	1048, 1088, 1218, 1261, 1381, 2060, 2666, 2934
BseRI	GAGGAG	1 cut(s)	6
BseSI	GKGCMC	1 cut(s)	1200
BseXI	GCAGC	5 cut(s)	434, 814, 817, 1141, 1282
Bsh1236I	CGCG	1 cut(s)	2280
Bsh1285I	CGRYCG	1 cut(s)	57
BshFI	GGCC	6 cut(s)	548, 1033, 1198, 1999, 2160, 2607
BshNI	GGYRCC	1 cut(s)	1739
BsiEI	CGRYCG	1 cut(s)	57
BsiHKAI	GWGCWC	1 cut(s)	565
BsiHKCI	CYCGRG	2 cut(s)	373, 1735
BsiSI	CCGG	1 cut(s)	1996
BslFI	GGGAC	2 cut(s)	128, 2312
BslI	CCNNNNNNNGG	9 cut(s)	108, 219, 462, 506, 613, 728, 928, 1712, 2324
BsmAI	GTCTC	4 cut(s)	674, 1464, 2449, 2920
BsmFI	GGGAC	2 cut(s)	128, 2312
BsmI	GAATGC	1 cut(s)	2528
BsnI	GGCC	6 cut(s)	548, 1033, 1198, 1999, 2160, 2607
BsoBI	CYCGRG	2 cut(s)	373, 1735
Bsp120I	GGGCCC	1 cut(s)	1196
Bsp1286I	GDGCHC	3 cut(s)	565, 1200, 2954
Bsp1407I	TGTACA	1 cut(s)	1488
Bsp1720I	GCTNAGC	1 cut(s)	2776
Bsp19I	CCATGG	2 cut(s)	879, 2217
Bsp68I	TCGCGA	1 cut(s)	2280
BspACI	CCGC	5 cut(s)	370, 1961, 3034, 3073, 3076
BspANI	GGCC	6 cut(s)	548, 1033, 1198, 1999, 2160, 2607
BspCNI	CTCAG	6 cut(s)	676, 700, 1315, 1474, 1558, 1984
BspFNI	CGCG	1 cut(s)	2280
BspHI	TCATGA	3 cut(s)	1243, 1558, 3085
BspLI	GGNNCC	7 cut(s)	144, 202, 920, 1198, 1741, 1959, 2413
BspMAI	CTGCAG	1 cut(s)	835
BspMI	ACCTGC	1 cut(s)	1742
BspOI	GCTAGC	1 cut(s)	1761
BspPI	GGATC	6 cut(s)	515, 1387, 2403, 2406, 2419, 2987
BspT107I	GGYRCC	1 cut(s)	1739
BspTI	CTTAAG	1 cut(s)	1621
BsrBI	CCGCTC	1 cut(s)	372
BsrGI	TGTACA	1 cut(s)	1488
BsrI	ACTGG	8 cut(s)	1048, 1088, 1218, 1261, 1381, 2060, 2666, 2934
BssECI	CCNNGG	8 cut(s)	106, 217, 879, 2217, 2487, 2542, 2571, 2852
BssNAI	GTATAC	1 cut(s)	1780
BssNI	GRCGYC	2 cut(s)	162, 2593
BssT1I	CCWWGG	5 cut(s)	106, 879, 2217, 2542, 2571
Bst1107I	GTATAC	1 cut(s)	1780
Bst2UI	CCWGG	7 cut(s)	218, 545, 608, 770, 916, 2609, 2854
Bst4CI	ACNGT	7 cut(s)	181, 1410, 1522, 1696, 2047, 2422, 2506
Bst6I	CTCTTC	4 cut(s)	32, 1502, 2631, 2745
BstACI	GRCGYC	2 cut(s)	162, 2593
BstAFI	CTTAAG	1 cut(s)	1621
BstAPI	GCANNNNNTGC	1 cut(s)	1013
BstAUI	TGTACA	1 cut(s)	1488
BstBAI	YACGTR	1 cut(s)	2054
BstC8I	GCNNGC	1 cut(s)	1759
BstDSI	CCRYGG	2 cut(s)	879, 2217
BstF5I	GGATG	9 cut(s)	173, 213, 515, 778, 964, 1508, 1519, 2431, 2656
BstFNI	CGCG	1 cut(s)	2280
BstH2I	RGCGCY	1 cut(s)	319
BstHHI	GCGC	1 cut(s)	318
BstMAI	GTCTC	4 cut(s)	674, 1464, 2449, 2920
BstMCI	CGRYCG	1 cut(s)	57
BstMWI	GCNNNNNNNGC	5 cut(s)	266, 545, 1013, 1748, 2842
BstNI	CCWGG	7 cut(s)	218, 545, 608, 770, 916, 2609, 2854
BstNSI	RCATGY	3 cut(s)	406, 2138, 2362
BstPAI	GACNNNNGTC	1 cut(s)	636
BstSCI	CCNGG	7 cut(s)	216, 543, 606, 768, 914, 2607, 2852
BstSFI	CTRYAG	2 cut(s)	831, 2284
BstSLI	GKGCMC	1 cut(s)	1200
BstUI	CGCG	1 cut(s)	2280
BstV1I	GCAGC	5 cut(s)	434, 814, 817, 1141, 1282
BstV2I	GAAGAC	1 cut(s)	182
BstX2I	RGATCY	2 cut(s)	1379, 2411
BstXI	CCANNNNNNTGG	3 cut(s)	88, 1680, 2422
BstYI	RGATCY	2 cut(s)	1379, 2411
BstZ17I	GTATAC	1 cut(s)	1780
BsuI	GTATCC	4 cut(s)	1085, 1304, 1708, 2091
BsuRI	GGCC	6 cut(s)	548, 1033, 1198, 1999, 2160, 2607
BtgI	CCRYGG	2 cut(s)	879, 2217
BtgZI	GCGATG	1 cut(s)	452
BtrI	CACGTC	1 cut(s)	965
BtsCI	GGATG	9 cut(s)	173, 213, 515, 778, 964, 1508, 1519, 2431, 2656
BtsI	GCAGTG	1 cut(s)	2211
BtsIMutI	CAGTG	6 cut(s)	1211, 1254, 1415, 2211, 2241, 2427
BtuMI	TCGCGA	1 cut(s)	2280
BveI	ACCTGC	1 cut(s)	1742
Cac8I	GCNNGC	1 cut(s)	1759
CciI	TCATGA	3 cut(s)	1243, 1558, 3085
CfoI	GCGC	1 cut(s)	318
Cfr13I	GGNCC	8 cut(s)	142, 361, 876, 1196, 1197, 2057, 2159, 2984
CseI	GACGC	1 cut(s)	2582
CsiI	ACCWGGT	1 cut(s)	914
Csp6I	GTAC	6 cut(s)	979, 1188, 1260, 1489, 1703, 2075
CviQI	GTAC	6 cut(s)	979, 1188, 1260, 1489, 1703, 2075
DraI	TTTAAA	1 cut(s)	1177
DriI	GACNNNNNGTC	1 cut(s)	2537
EaeI	YGGCCR	2 cut(s)	1031, 2605
Eam1104I	CTCTTC	4 cut(s)	32, 1502, 2631, 2745
Eam1105I	GACNNNNNGTC	1 cut(s)	2537
EarI	CTCTTC	4 cut(s)	32, 1502, 2631, 2745
Eco130I	CCWWGG	5 cut(s)	106, 879, 2217, 2542, 2571
Eco147I	AGGCCT	1 cut(s)	548
Eco24I	GRGCYC	2 cut(s)	1200, 2954
Eco47I	GGWCC	5 cut(s)	142, 361, 876, 2057, 2984
Eco47III	AGCGCT	1 cut(s)	317
Eco57I	CTGAAG	4 cut(s)	1176, 2542, 2726, 2858
Eco88I	CYCGRG	2 cut(s)	373, 1735
EcoO109I	RGGNCCY	1 cut(s)	1196
EcoRI	GAATTC	5 cut(s)	386, 485, 1527, 1975, 2668
EcoRII	CCWGG	7 cut(s)	216, 543, 606, 768, 914, 2607, 2852
EcoT14I	CCWWGG	5 cut(s)	106, 879, 2217, 2542, 2571
EcoT22I	ATGCAT	1 cut(s)	414
EcoT38I	GRGCYC	2 cut(s)	1200, 2954
ErhI	CCWWGG	5 cut(s)	106, 879, 2217, 2542, 2571
FalI	AAGNNNNNCTT	4 cut(s)	1174, 1206, 1631, 1663
FaqI	GGGAC	2 cut(s)	128, 2312
FauNDI	CATATG	1 cut(s)	1987
FbaI	TGATCA	2 cut(s)	184, 448
FblI	GTMKAC	3 cut(s)	1414, 1779, 1805
Fnu4HI	GCNGC	6 cut(s)	423, 828, 831, 1155, 1271, 3076
FokI	GGATG	9 cut(s)	180, 200, 522, 785, 971, 1515, 1526, 2438, 2663
FriOI	GRGCYC	2 cut(s)	1200, 2954
Fsp4HI	GCNGC	6 cut(s)	423, 828, 831, 1155, 1271, 3076
GlaI	GCGC	1 cut(s)	317
GluI	GCNGC	6 cut(s)	423, 828, 831, 1155, 1271, 3076
GsuI	CTGGAG	1 cut(s)	662
HaeII	RGCGCY	1 cut(s)	319
HaeIII	GGCC	6 cut(s)	548, 1033, 1198, 1999, 2160, 2607
HapII	CCGG	1 cut(s)	1996
HgaI	GACGC	1 cut(s)	2582
HhaI	GCGC	1 cut(s)	318
Hin1I	GRCGYC	2 cut(s)	162, 2593
Hin6I	GCGC	1 cut(s)	316
HinP1I	GCGC	1 cut(s)	316
HincII	GTYRAC	4 cut(s)	380, 439, 952, 1806
HindII	GTYRAC	4 cut(s)	380, 439, 952, 1806
HindIII	AAGCTT	2 cut(s)	1888, 2843
HpaII	CCGG	1 cut(s)	1996
HphI	GGTGA	2 cut(s)	1058, 2613
Hpy99I	CGWCG	4 cut(s)	161, 164, 167, 2598
HpyAV	CCTTC	5 cut(s)	735, 999, 1176, 1739, 2939
HpyCH4III	ACNGT	7 cut(s)	181, 1410, 1522, 1696, 2047, 2422, 2506
HpyCH4IV	ACGT	8 cut(s)	132, 162, 294, 964, 1125, 1275, 2053, 2234
HpyF10VI	GCNNNNNNNGC	5 cut(s)	266, 545, 1013, 1748, 2842
HpySE526I	ACGT	8 cut(s)	132, 162, 294, 964, 1125, 1275, 2053, 2234
Hsp92I	GRCGYC	2 cut(s)	162, 2593
HspAI	GCGC	1 cut(s)	316
Ksp22I	TGATCA	2 cut(s)	184, 448
LmnI	GCTCC	6 cut(s)	206, 560, 1963, 2131, 2746, 3055
Lsp1109I	GCAGC	5 cut(s)	434, 814, 817, 1141, 1282
LweI	GCATC	8 cut(s)	328, 475, 994, 1210, 1237, 1376, 1451, 2433
MabI	ACCWGGT	1 cut(s)	914
MaeII	ACGT	8 cut(s)	132, 162, 294, 964, 1125, 1275, 2053, 2234
MaeIII	GTNAC	8 cut(s)	355, 493, 1046, 1252, 1450, 1584, 2197, 2400
MbiI	CCGCTC	1 cut(s)	372
MfeI	CAATTG	1 cut(s)	1230
MflI	RGATCY	2 cut(s)	1379, 2411
MhlI	GDGCHC	3 cut(s)	565, 1200, 2954
MlsI	TGGCCA	2 cut(s)	1033, 2607
MluNI	TGGCCA	2 cut(s)	1033, 2607
MmeI	TCCRAC	6 cut(s)	145, 222, 228, 550, 1685, 2776
Mox20I	TGGCCA	2 cut(s)	1033, 2607
Mph1103I	ATGCAT	1 cut(s)	414
MroXI	GAANNNNTTC	3 cut(s)	1905, 2846, 3065
MscI	TGGCCA	2 cut(s)	1033, 2607
MslI	CAYNNNNRTG	6 cut(s)	407, 1245, 2216, 3084, 3087, 3090
Msp20I	TGGCCA	2 cut(s)	1033, 2607
MspA1I	CMGCKG	1 cut(s)	1963
MspCI	CTTAAG	1 cut(s)	1621
MspI	CCGG	1 cut(s)	1996
MspR9I	CCNGG	7 cut(s)	218, 545, 608, 770, 916, 2609, 2854
MunI	CAATTG	1 cut(s)	1230
Mva1269I	GAATGC	1 cut(s)	2528
MvaI	CCWGG	7 cut(s)	218, 545, 608, 770, 916, 2609, 2854
MvnI	CGCG	1 cut(s)	2280
MwoI	GCNNNNNNNGC	5 cut(s)	266, 545, 1013, 1748, 2842
NcoI	CCATGG	2 cut(s)	879, 2217
NdeI	CATATG	1 cut(s)	1987
NheI	GCTAGC	1 cut(s)	1757
NlaIV	GGNNCC	7 cut(s)	144, 202, 920, 1198, 1741, 1959, 2413
NmeAIII	GCCGAG	1 cut(s)	2512
NmuCI	GTSAC	6 cut(s)	355, 493, 1046, 1252, 1584, 2400
NruI	TCGCGA	1 cut(s)	2280
NsiI	ATGCAT	1 cut(s)	414
NspI	RCATGY	3 cut(s)	406, 2138, 2362
PagI	TCATGA	3 cut(s)	1243, 1558, 3085
PaqCI	CACCTGC	1 cut(s)	1742
PceI	AGGCCT	1 cut(s)	548
PctI	GAATGC	1 cut(s)	2528
PdmI	GAANNNNTTC	3 cut(s)	1905, 2846, 3065
PflFI	GACNNNGTC	3 cut(s)	161, 365, 1520
PflMI	CCANNNNNTGG	2 cut(s)	506, 1712
PfoI	TCCNGGA	1 cut(s)	768
PkrI	GCNGC	6 cut(s)	424, 829, 832, 1156, 1272, 3077
Ppu21I	YACGTR	1 cut(s)	2054
PshAI	GACNNNNGTC	1 cut(s)	636
PshBI	ATTAAT	3 cut(s)	1074, 2088, 2343
PsiI	TTATAA	2 cut(s)	1929, 2735
Psp1406I	AACGTT	1 cut(s)	2234
Psp6I	CCWGG	7 cut(s)	216, 543, 606, 768, 914, 2607, 2852
PspGI	CCWGG	7 cut(s)	216, 543, 606, 768, 914, 2607, 2852
PspN4I	GGNNCC	7 cut(s)	144, 202, 920, 1198, 1741, 1959, 2413
PspOMI	GGGCCC	1 cut(s)	1196
PspPI	GGNCC	8 cut(s)	142, 361, 876, 1196, 1197, 2057, 2159, 2984
PstI	CTGCAG	1 cut(s)	835
PsuI	RGATCY	2 cut(s)	1379, 2411
PsyI	GACNNNGTC	3 cut(s)	161, 365, 1520
RruI	TCGCGA	1 cut(s)	2280
RsaI	GTAC	6 cut(s)	980, 1189, 1261, 1490, 1704, 2076
RsaNI	GTAC	6 cut(s)	979, 1188, 1260, 1489, 1703, 2075
RseI	CAYNNNNRTG	6 cut(s)	407, 1245, 2216, 3084, 3087, 3090
SalI	GTCGAC	1 cut(s)	1804
SatI	GCNGC	6 cut(s)	423, 828, 831, 1155, 1271, 3076
Sau96I	GGNCC	8 cut(s)	142, 361, 876, 1196, 1197, 2057, 2159, 2984
ScaI	AGTACT	1 cut(s)	1189
ScrFI	CCNGG	7 cut(s)	218, 545, 608, 770, 916, 2609, 2854
SduI	GDGCHC	3 cut(s)	565, 1200, 2954
SexAI	ACCWGGT	1 cut(s)	914
SfaNI	GCATC	8 cut(s)	328, 475, 994, 1210, 1237, 1376, 1451, 2433
SfcI	CTRYAG	2 cut(s)	831, 2284
SinI	GGWCC	5 cut(s)	142, 361, 876, 2057, 2984
SmiMI	CAYNNNNRTG	6 cut(s)	407, 1245, 2216, 3084, 3087, 3090
SmlI	CTYRAG	2 cut(s)	1621, 2473
SmoI	CTYRAG	2 cut(s)	1621, 2473
SpeI	ACTAGT	2 cut(s)	1330, 3004
SseBI	AGGCCT	1 cut(s)	548
SsiI	CCGC	5 cut(s)	370, 1961, 3034, 3073, 3076
StuI	AGGCCT	1 cut(s)	548
StyD4I	CCNGG	7 cut(s)	216, 543, 606, 768, 914, 2607, 2852
StyI	CCWWGG	5 cut(s)	106, 879, 2217, 2542, 2571
TaaI	ACNGT	7 cut(s)	181, 1410, 1522, 1696, 2047, 2422, 2506
TaiI	ACGT	8 cut(s)	135, 165, 297, 967, 1128, 1278, 2056, 2237
TaqI	TCGA	6 cut(s)	489, 576, 595, 856, 1581, 1805
TaqII	GACCGA	2 cut(s)	43, 2388
TatI	WGTACW	4 cut(s)	978, 1187, 1488, 2074
TauI	GCSGC	1 cut(s)	3078
TscAI	CASTG	6 cut(s)	1218, 1261, 1415, 2218, 2248, 2427
TseFI	GTSAC	6 cut(s)	355, 493, 1046, 1252, 1584, 2400
TseI	GCWGC	5 cut(s)	422, 827, 830, 1154, 1270
Tsp45I	GTSAC	6 cut(s)	355, 493, 1046, 1252, 1584, 2400
TspGWI	ACGGA	6 cut(s)	374, 497, 911, 1220, 1690, 2978
TspRI	CASTG	6 cut(s)	1218, 1261, 1415, 2218, 2248, 2427
Tth111I	GACNNNGTC	3 cut(s)	161, 365, 1520
Van91I	CCANNNNNTGG	2 cut(s)	506, 1712
Vha464I	CTTAAG	1 cut(s)	1621
VpaK11BI	GGWCC	5 cut(s)	142, 361, 876, 2057, 2984
VspI	ATTAAT	3 cut(s)	1074, 2088, 2343
XbaI	TCTAGA	1 cut(s)	1600
XceI	RCATGY	3 cut(s)	406, 2138, 2362
XcmI	CCANNNNNNNNNTGG	2 cut(s)	507, 2321
XmaJI	CCTAGG	1 cut(s)	2542
XmiI	GTMKAC	3 cut(s)	1414, 1779, 1805
XmnI	GAANNNNTTC	3 cut(s)	1905, 2846, 3065
ZraI	GACGTC	1 cut(s)	163
ZrmI	AGTACT	1 cut(s)	1189
Zsp2I	ATGCAT	1 cut(s)	414

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.