Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001301

Physical Location & Seq

Genomic Coordinates Forward (+)

27538850 .. 27552736

Gene Structure 13887 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1131600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3162 bp

ATGAACACTTTTCTGGGGTCTCAGTGCATTCCGTCACCGACACCGACACCGACACCATTAATTTGCCACGACATGGCGGCCGTTGAAGCTCGCTGCCGTGTCCCACGGCTTCTGTTGTTAGTAGTAGTAGTAGTACTAGTTGTGTTTGAGTTTATTCTGTATGCGGAGTCCAAGTACGTGCAGTACAATACGACGTCGGGGCTTGTCGCCGGGAAGCTGAACGTGCATTTGGTCCCTCACAGCCACGACGACGTGGGATGGTTGAAGACCATCGATCAGTACTACGTTGGCTCCAATAATTCCATTCAGGGAGCTTGCGTTCAGAACGTGCTGGATTCTCTGGTTCCGGCATTGTTGGCCGATAAGAACCGCAAGTTCATATATGTTGAGCAGGCTTTTTTCCAACGTTGGTGGAGAGAGCAGAGCGAGGGGACTCAGAACATAGTGAAACAGCTAGTCAACGACGGTCAACTTGAACTCATAAATGGGGGGATGTGCATGCACGATGAGGCTGCTACTCATTACATTGACATGATTGATCAGACGACTCTCGGGCATCGGTTTATCAAACAAGAGTTCAATGTGACTCCGAGGATTGGATGGCAGATCGACCCGTTTGGACATTCTGCAGTGCAGGCTTACCTGTTGGGAGCTGAGGTTGGATTCGACTCTCTTTTCTTTGCAAGAATCGACTACCAAGACAGAGCTAAACGGATGGTTGACAAAAGCCTCGAGGTTGTCTGGCGGGGTTCTAAGAGTCTCGGTTCATCTGCATGTTGGATCGTCTATTGCCTTATTTTCACAGGCGTATTTCCTAAGAATTATGAACCTCCTTCTGATAATTTCTACTTTGAAGTTGATGACCCTTCACCTATTGTACAAGATGATACGTTTCTGTTCGACTACAATGTGCCGGATCGCGTTAATGACTTTGTATCAGCTGCAATGTCACAAGCAAACATCACGCGTACGAATCATATCATGTGGACTATGGGAACGGACTTCAAGTATCAATACGCTAATACATGGTTCCGACAGATGGATAAGTTCATCCATTATGTGAATCAAGATGGGCGTGTCAATGCGCTATATTCAACCCCGTCTATATACACTGATGCCAAGTATGCAGCAAATGAATCCTGGCCAATCAAGACCGATGACTTCTTTCCATACGCTGACGTTGTGAATGCTTACTGGACTGGATATTTCACGAGCAGGCCAGCCATCAAAGGATATGTTAGAACACTGGGCGGCTACTATATGGCAGCAAGACAATTGGAGTTTTTCAAGGGGTTGAACAAGTCGGGGCCTAACACAGATTCGTTGGCTGATGCTTTAGCGATTGTTCAACATCATGATGCAGTTAGTGGCACAGAGAAGCAGCACGTGGCGTATGATTATGCAAAACGGCTCTCAATAGGTTACAAGGAGGCGGAGCAGGTTGTTGCGGAATCACTTGCATGCTTGACAGAGTCAAAATCAGAAGCTGGTTGCAAGAGTTCATCAACTAAGTTTCAACAGTGTCCACTTCTAAATATTAGCTATTGTCCCCCATCAGAAGCTGATCTATCAAATGGGAAAACCCTGGTTATTGTTGTCTACAACTCTCTAGGATGGAAGAGAGAGGACATCGTAAAGATTCCTGTTGTCAGTGCAAACGTCACTGTAAGGGATTACACAGGAAAAGAAGTCGAATCACAAATCTTACCTCTGCTTAAGGCATCAGCGGGTCTAAGAAACAATTATGTTAAGGCATATCTGGGTGTATCACCAAGTATCACACCAAGTTACTGGCTTGCATTTTCAGCAACTGTACCACCTCTTGGTTTTAGCACTTATACCGTGTCAAGTGCCACACGAACAGCTACAACTTCAGAGAGACAAACTGCTTACAAGTCAGAAACGAGTCAGAATGATACCATAGAAGTCGGGCCAGGAAAATTGAAACTTGTTTATTCTGGAAATGATGGAAAACTTACTCAATACATCAACACTGGAAGCTTGGTCAACAAGTCGATACAACAAGCTTTTAGTTATTACCCTGGAGATGATGGACATCATGGAAGCGCGGATAAACAGGCCTCGGGAGCTTATATCTTCCGCCCTAATGGTACAGTCCCCATTAACTCCGAAGGGCAGGTTTCTTTTACTGTGCTGAGGGGACCGTTGTTAGATGAAGTACATCAGAGGATCAACTCTTGGATATATCAGGTCACTAGATTGTACAAGGAAAAGGAGCATGCTGAGATTGAATTTACGGTTGGGCCTATACCAATCGATGATGGAATTGGCAAAGAAATCGTGACTAAGATTACAACTGGCATGGAAACCAACAAAAAGTTCTACACAGATTCTAACGCGCGCGATTTTATTGAAAGAATTCGAGACTATAGAAAGGATTGGAACTTGGAATTGAATCAACCCGTCGCAGGAAATTATTATCCGATTAATCTCGGACTCTACACGAAAGACAACAACACGGAGTTTTCAGTATTAGTAGATAGATCTGTGGGTGGATCCAGCATTGTGGATGGGCAATTGGAACTCATGGTTCATAGGAGGTTGACCCATGATGATGACAGAGGCGTTGGAGAAGCTCTAAACGAAACTGTGTGCATTCAAGATGGTTGCAAAGGATTAACAATCATCGGAAAGTACTACCTCAGACTCGATCCATTGGGAGAGGGTGCTAAATGGCGCCGATCTTTTGGTCAGGAGATATATTCTCCATTTCTTTTAGCCTTCACAGAACAAGATGAAGATAGCTGGAAAAGCTCTCATGTCACAACCTTTTCGGGAATGGATCCTTCCTACACTTTAACTGATAATGTCGCGATAATAACCCTCCAGGAGCTGGAAGATGGAAAACTTCTGTTTCGCCTTGCACATTTATACGAGATTGACGAGGACAAGGATCTTTCGGTTATGGCAAGTGTAGAGCTCAAAAAAGTGTTTGCAGATAAGAAGATTAGAAAAGTAACGGAAATGAGTTTATCTGCAAACCAAGAAAGAGCGGAAATGGAGAAGAAGAGACTAGTGTGGAAAGCAGAAGGCTCCTCCGACAAAGAAGCCAAAGTGTTAAGGGGAGGACCTGTTGATCCAACAAAGCTGGTGGTTGATCTTGCTCCAATGGAAATTCGAACGCTTATCATCGACTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Alpha-mann_mid Glyco_hydro_38 Glyco_hydro_38C

Protein Analysis

1054

Amino Acids

118.33

Weight (kDa)

5.58

Isoelectric Point (pI)

35.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	74 - 392	1.9e-80	Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid	PF09261	397 - 489	5.6e-20	Alpha mannosidase middle domain
Laman-like_dom	PF21260	564 - 615	3.8e-08	Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C	PF07748	646 - 861	1.8e-41	Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2	PF17677	943 - 1049	8.8e-07	Glycosyl hydrolases family 38 C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001301)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13980 AT5G13980 AT5G13980
fragaria_vesca	FvH4_5g09420 FvH4_5g09421 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272
malus_domestica	MD06G1131600.v1.1 MD09G1002900.v1.1 MD17G1007000.v1.1
prunus_persica	Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.5G142600_v2.0.a1 Prupe.5G142600_v2.0.a1
pyrus_communis	pycom06g12300 pycom06g12310 pycom111g00240 pycom17g00260
rosa_chinensis	RchiOBHm_Chr2g0176471 RchiOBHm_Chr2g0176481 RchiOBHm_Chr7g0188771 RchiOBHm_Chr7g0188781
rosa_laevigata	RLG00000004661 RLG00000004662 RLG00000022416
rosa_multiflora	Rmu_sc0000303.1_g000057 Rmu_sc0003642.1_g000008 Rmu_sc0011904.1_g000012
rosa_roxburghii	Rroxscaffold_2G00076370 Rroxscaffold_3G00266040 Rroxscaffold_3G00266050
rosa_rugosa	Rorug02G0595000 Rorug06G0493700
rosa_samantha	Rh2AG673300 Rh2BG684600 Rh2CG647400 Rh2DG698200 Rh7AG098300 Rh7AG098400 Rh7BG099900 Rh7BG100200 Rh7CG101600 Rh7CG101700 Rh7DG100100 Rh7DG100200
rosa_wichuraiana	Rw0G010390 Rw2G055220 Rw7G008480 Rw7G008490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	197
AbsI	CCTCGAGG	1 cut(s)	731
Acc36I	ACCTGC	2 cut(s)	1429, 2128
AccB1I	GGYRCC	1 cut(s)	2697
AccB7I	CCANNNNNTGG	3 cut(s)	73, 1823, 2854
AccBSI	CCGCTC	1 cut(s)	3014
AccI	GTMKAC	1 cut(s)	1599
AccII	CGCG	7 cut(s)	921, 967, 2069, 2360, 2362, 2364, 2834
AclI	AACGTT	1 cut(s)	406
AcoI	YGGCCR	3 cut(s)	78, 357, 1142
AcsI	RAATTY	3 cut(s)	2252, 2379, 3135
AcuI	CTGAAG	1 cut(s)	1857
AcvI	CACGTG	1 cut(s)	1387
AcyI	GRCGYC	2 cut(s)	194, 2698
AfiI	CCNNNNNNNGG	6 cut(s)	73, 596, 1039, 1823, 2429, 2854
AflII	CTTAAG	1 cut(s)	1715
AflIII	ACRYGT	1 cut(s)	965
AhlI	ACTAGT	2 cut(s)	136, 3034
AjiI	CACGTC	1 cut(s)	253
AjnI	CCWGG	5 cut(s)	1139, 1584, 1933, 2041, 2847
AjuI	GAANNNNNNNTTGG	6 cut(s)	212, 244, 2244, 2276, 2324, 2356
AloI	GAACNNNNNNTCC	2 cut(s)	303, 335
Alw21I	GWGCWC	1 cut(s)	2943
Alw26I	GTCTC	5 cut(s)	24, 764, 1873, 2379, 3025
AlwNI	CAGNNNCTG	4 cut(s)	1487, 1562, 1811, 2854
Ama87I	CYCGRG	3 cut(s)	551, 731, 2083
AoxI	GGCC	8 cut(s)	78, 357, 1142, 1217, 1307, 1931, 2079, 2264
ApeKI	GCWGC	6 cut(s)	93, 512, 941, 1127, 1265, 1381
ApoI	RAATTY	3 cut(s)	2252, 2379, 3135
AseI	ATTAAT	2 cut(s)	59, 2448
Asp700I	GAANNNNTTC	1 cut(s)	2379
AspLEI	GCGC	5 cut(s)	1087, 2069, 2362, 2364, 2700
AspS9I	GGNCC	6 cut(s)	232, 1307, 1931, 2162, 2264, 3089
AsuC2I	CCSGG	1 cut(s)	211
AsuHPI	GGTGA	3 cut(s)	27, 861, 1761
AsuII	TTCGAA	1 cut(s)	3139
AvaI	CYCGRG	3 cut(s)	551, 731, 2083
AvaII	GGWCC	3 cut(s)	232, 2162, 3089
BalI	TGGCCA	1 cut(s)	1144
BamHI	GGATCC	2 cut(s)	2516, 2803
BanI	GGYRCC	1 cut(s)	2697
BanII	GRGCYC	1 cut(s)	2943
BauI	CACGAG	1 cut(s)	1210
BbrPI	CACGTG	1 cut(s)	1387
BbsI	GAAGAC	1 cut(s)	272
Bbv12I	GWGCWC	1 cut(s)	2943
BbvCI	CCTCAGC	2 cut(s)	654, 2156
BbvI	GCAGC	6 cut(s)	80, 499, 928, 1139, 1277, 1393
BceAI	ACGGC	4 cut(s)	65, 81, 122, 1424
BcgI	CGANNNNNNTGC	4 cut(s)	494, 528, 2281, 2315
BciT130I	CCWGG	5 cut(s)	1141, 1586, 1935, 2043, 2849
BclI	TGATCA	1 cut(s)	538
BcnI	CCSGG	1 cut(s)	211
BcoDI	GTCTC	5 cut(s)	24, 764, 1873, 2379, 3025
BcuI	ACTAGT	2 cut(s)	136, 3034
BfaI	CTAG	5 cut(s)	137, 455, 1610, 2217, 3035
BfmI	CTRYAG	2 cut(s)	627, 2389
BfoI	RGCGCY	1 cut(s)	2701
BfrI	CTTAAG	1 cut(s)	1715
BfuAI	ACCTGC	2 cut(s)	1429, 2128
BglII	AGATCT	1 cut(s)	2504
BisI	GCNGC	8 cut(s)	78, 94, 513, 942, 1128, 1252, 1266, 1382
BlsI	GCNGC	8 cut(s)	79, 95, 514, 943, 1129, 1253, 1267, 1383
BmcAI	AGTACT	3 cut(s)	135, 281, 2657
Bme1390I	CCNGG	6 cut(s)	211, 1141, 1586, 1935, 2043, 2849
Bme18I	GGWCC	3 cut(s)	232, 2162, 3089
BmeT110I	CYCGRG	3 cut(s)	551, 731, 2083
BmgBI	CACGTC	1 cut(s)	253
BmgT120I	GGNCC	6 cut(s)	232, 1307, 1931, 2162, 2264, 3089
BmrFI	CCNGG	6 cut(s)	211, 1141, 1586, 1935, 2043, 2849
BmrI	ACTGGG	1 cut(s)	1256
BmsI	GCATC	5 cut(s)	565, 1105, 1321, 1348, 1730
BmuI	ACTGGG	1 cut(s)	1256
BpiI	GAAGAC	1 cut(s)	272
BpmI	CTGGAG	2 cut(s)	2064, 2831
Bpu10I	CCTNAGC	2 cut(s)	654, 2156
Bpu14I	TTCGAA	1 cut(s)	3139
BpuMI	CCSGG	1 cut(s)	211
Bsa29I	ATCGAT	2 cut(s)	273, 2277
BsaAI	YACGTR	2 cut(s)	178, 1387
BsaBI	GATNNNNATC	1 cut(s)	2055
BsaHI	GRCGYC	2 cut(s)	194, 2698
BsaI	GGTCTC	1 cut(s)	24
BsaJI	CCNNGG	5 cut(s)	104, 590, 1584, 2041, 2082
Bsc4I	CCNNNNNNNGG	6 cut(s)	73, 596, 1039, 1823, 2429, 2854
Bse1I	ACTGG	6 cut(s)	1199, 1204, 1251, 1796, 1999, 2323
Bse3DI	GCAATG	1 cut(s)	951
Bse8I	GATNNNNATC	1 cut(s)	2055
BseBI	CCWGG	5 cut(s)	1141, 1586, 1935, 2043, 2849
BseCI	ATCGAT	2 cut(s)	273, 2277
BseDI	CCNNGG	5 cut(s)	104, 590, 1584, 2041, 2082
BseGI	GGATG	7 cut(s)	263, 498, 605, 720, 1050, 1619, 2536
BseJI	GATNNNNATC	1 cut(s)	2055
BseLI	CCNNNNNNNGG	6 cut(s)	73, 596, 1039, 1823, 2429, 2854
BseMI	GCAATG	1 cut(s)	951
BseMII	CTCAG	6 cut(s)	35, 449, 645, 2147, 2235, 2677
BseNI	ACTGG	6 cut(s)	1199, 1204, 1251, 1796, 1999, 2323
BsePI	GCGCGC	1 cut(s)	2360
BseRI	GAGGAG	1 cut(s)	3046
BseX3I	CGGCCG	1 cut(s)	78
BseXI	GCAGC	6 cut(s)	80, 499, 928, 1139, 1277, 1393
BsgI	GTGCAG	2 cut(s)	200, 653
Bsh1236I	CGCG	7 cut(s)	921, 967, 2069, 2360, 2362, 2364, 2834
Bsh1285I	CGRYCG	1 cut(s)	81
BshFI	GGCC	8 cut(s)	80, 359, 1144, 1219, 1309, 1933, 2081, 2266
BshNI	GGYRCC	1 cut(s)	2697
BshVI	ATCGAT	2 cut(s)	273, 2277
BsiEI	CGRYCG	1 cut(s)	81
BsiHKAI	GWGCWC	1 cut(s)	2943
BsiHKCI	CYCGRG	3 cut(s)	551, 731, 2083
BsiSI	CCGG	3 cut(s)	210, 347, 914
BsiWI	CGTACG	1 cut(s)	968
BslFI	GGGAC	6 cut(s)	86, 218, 445, 1533, 2102, 2175
BslI	CCNNNNNNNGG	6 cut(s)	73, 596, 1039, 1823, 2429, 2854
BsmAI	GTCTC	5 cut(s)	24, 764, 1873, 2379, 3025
BsmFI	GGGAC	6 cut(s)	86, 218, 445, 1533, 2102, 2175
BsmI	GAATGC	3 cut(s)	27, 1192, 2616
BsnI	GGCC	8 cut(s)	80, 359, 1144, 1219, 1309, 1933, 2081, 2266
Bso31I	GGTCTC	1 cut(s)	24
BsoBI	CYCGRG	3 cut(s)	551, 731, 2083
Bsp119I	TTCGAA	1 cut(s)	3139
Bsp1286I	GDGCHC	1 cut(s)	2943
Bsp1407I	TGTACA	2 cut(s)	877, 2223
Bsp68I	TCGCGA	1 cut(s)	2834
BspANI	GGCC	8 cut(s)	80, 359, 1144, 1219, 1309, 1933, 2081, 2266
BspCNI	CTCAG	6 cut(s)	34, 448, 646, 2148, 2236, 2676
BspDI	ATCGAT	2 cut(s)	273, 2277
BspFNI	CGCG	7 cut(s)	921, 967, 2069, 2360, 2362, 2364, 2834
BspHI	TCATGA	1 cut(s)	1354
BspMAI	CTGCAG	1 cut(s)	631
BspMI	ACCTGC	2 cut(s)	1429, 2128
BspT104I	TTCGAA	1 cut(s)	3139
BspT107I	GGYRCC	1 cut(s)	2697
BspTI	CTTAAG	1 cut(s)	1715
BspTNI	GGTCTC	1 cut(s)	24
BsrBI	CCGCTC	1 cut(s)	3014
BsrDI	GCAATG	1 cut(s)	951
BsrGI	TGTACA	2 cut(s)	877, 2223
BsrI	ACTGG	6 cut(s)	1199, 1204, 1251, 1796, 1999, 2323
BssECI	CCNNGG	5 cut(s)	104, 590, 1584, 2041, 2082
BssHII	GCGCGC	1 cut(s)	2360
BssNI	GRCGYC	2 cut(s)	194, 2698
BssSI	CACGAG	1 cut(s)	1210
Bst2BI	CACGAG	1 cut(s)	1210
Bst2UI	CCWGG	5 cut(s)	1141, 1586, 1935, 2043, 2849
Bst6I	CTCTTC	2 cut(s)	1613, 3023
BstACI	GRCGYC	2 cut(s)	194, 2698
BstAFI	CTTAAG	1 cut(s)	1715
BstAUI	TGTACA	2 cut(s)	877, 2223
BstBAI	YACGTR	2 cut(s)	178, 1387
BstBI	TTCGAA	1 cut(s)	3139
BstDSI	CCRYGG	1 cut(s)	104
BstF5I	GGATG	7 cut(s)	263, 498, 605, 720, 1050, 1619, 2536
BstFNI	CGCG	7 cut(s)	921, 967, 2069, 2360, 2362, 2364, 2834
BstH2I	RGCGCY	1 cut(s)	2701
BstHHI	GCGC	5 cut(s)	1087, 2069, 2362, 2364, 2700
BstMAI	GTCTC	5 cut(s)	24, 764, 1873, 2379, 3025
BstMCI	CGRYCG	1 cut(s)	81
BstMWI	GCNNNNNNNGC	8 cut(s)	86, 223, 356, 635, 1124, 1805, 2087, 2772
BstNI	CCWGG	5 cut(s)	1141, 1586, 1935, 2043, 2849
BstNSI	RCATGY	4 cut(s)	502, 777, 1464, 2243
BstSCI	CCNGG	6 cut(s)	209, 1139, 1584, 1933, 2041, 2847
BstSFI	CTRYAG	2 cut(s)	627, 2389
BstUI	CGCG	7 cut(s)	921, 967, 2069, 2360, 2362, 2364, 2834
BstV1I	GCAGC	6 cut(s)	80, 499, 928, 1139, 1277, 1393
BstV2I	GAAGAC	1 cut(s)	272
BstX2I	RGATCY	4 cut(s)	2504, 2516, 2803, 2914
BstXI	CCANNNNNNTGG	2 cut(s)	1791, 2527
BstYI	RGATCY	4 cut(s)	2504, 2516, 2803, 2914
BstZI	CGGCCG	1 cut(s)	78
Bsu15I	ATCGAT	2 cut(s)	273, 2277
BsuRI	GGCC	8 cut(s)	80, 359, 1144, 1219, 1309, 1933, 2081, 2266
BsuTUI	ATCGAT	2 cut(s)	273, 2277
BtgI	CCRYGG	1 cut(s)	104
BtrI	CACGTC	1 cut(s)	253
BtsCI	GGATG	7 cut(s)	263, 498, 605, 720, 1050, 1619, 2536
BtsI	GCAGTG	1 cut(s)	636
BtsIMutI	CAGTG	8 cut(s)	29, 636, 1110, 1244, 1526, 1657, 1662, 1992
BtuMI	TCGCGA	1 cut(s)	2834
BveI	ACCTGC	2 cut(s)	1429, 2128
CaiI	CAGNNNCTG	4 cut(s)	1487, 1562, 1811, 2854
CciI	TCATGA	1 cut(s)	1354
CfoI	GCGC	5 cut(s)	1087, 2069, 2362, 2364, 2700
Cfr13I	GGNCC	6 cut(s)	232, 1307, 1931, 2162, 2264, 3089
ClaI	ATCGAT	2 cut(s)	273, 2277
CspCI	CAANNNNNGTGG	4 cut(s)	392, 427, 3093, 3128
DinI	GGCGCC	1 cut(s)	2699
EaeI	YGGCCR	3 cut(s)	78, 357, 1142
EagI	CGGCCG	1 cut(s)	78
Eam1104I	CTCTTC	2 cut(s)	1613, 3023
EarI	CTCTTC	2 cut(s)	1613, 3023
EciI	GGCGGA	2 cut(s)	1448, 2090
Ecl136II	GAGCTC	1 cut(s)	2941
EclXI	CGGCCG	1 cut(s)	78
Eco147I	AGGCCT	1 cut(s)	2081
Eco24I	GRGCYC	1 cut(s)	2943
Eco31I	GGTCTC	1 cut(s)	24
Eco47I	GGWCC	3 cut(s)	232, 2162, 3089
Eco52I	CGGCCG	1 cut(s)	78
Eco53kI	GAGCTC	1 cut(s)	2941
Eco57I	CTGAAG	1 cut(s)	1857
Eco72I	CACGTG	1 cut(s)	1387
Eco88I	CYCGRG	3 cut(s)	551, 731, 2083
EcoICRI	GAGCTC	1 cut(s)	2941
EcoO109I	RGGNCCY	2 cut(s)	1307, 3089
EcoRI	GAATTC	1 cut(s)	2379
EcoRII	CCWGG	5 cut(s)	1139, 1584, 1933, 2041, 2847
EcoT38I	GRGCYC	1 cut(s)	2943
EgeI	GGCGCC	1 cut(s)	2699
EheI	GGCGCC	1 cut(s)	2699
FaqI	GGGAC	6 cut(s)	86, 218, 445, 1533, 2102, 2175
FauI	CCCGC	2 cut(s)	738, 1720
FbaI	TGATCA	1 cut(s)	538
FblI	GTMKAC	1 cut(s)	1599
Fnu4HI	GCNGC	8 cut(s)	78, 94, 513, 942, 1128, 1252, 1266, 1382
FokI	GGATG	7 cut(s)	270, 505, 612, 727, 1037, 1626, 2543
FriOI	GRGCYC	1 cut(s)	2943
Fsp4HI	GCNGC	8 cut(s)	78, 94, 513, 942, 1128, 1252, 1266, 1382
FspBI	CTAG	5 cut(s)	137, 455, 1610, 2217, 3035
GlaI	GCGC	5 cut(s)	1086, 2068, 2361, 2363, 2699
GluI	GCNGC	8 cut(s)	78, 94, 513, 942, 1128, 1252, 1266, 1382
GsuI	CTGGAG	2 cut(s)	2064, 2831
HaeII	RGCGCY	1 cut(s)	2701
HaeIII	GGCC	8 cut(s)	80, 359, 1144, 1219, 1309, 1933, 2081, 2266
HapII	CCGG	3 cut(s)	210, 347, 914
HhaI	GCGC	5 cut(s)	1087, 2069, 2362, 2364, 2700
Hin1I	GRCGYC	2 cut(s)	194, 2698
Hin6I	GCGC	5 cut(s)	1085, 2067, 2360, 2362, 2698
HinP1I	GCGC	5 cut(s)	1085, 2067, 2360, 2362, 2698
HincII	GTYRAC	5 cut(s)	460, 470, 721, 2008, 2565
HindII	GTYRAC	5 cut(s)	460, 470, 721, 2008, 2565
HindIII	AAGCTT	2 cut(s)	1999, 2025
HpaII	CCGG	3 cut(s)	210, 347, 914
HphI	GGTGA	3 cut(s)	27, 861, 1761
Hpy166II	GTNNAC	8 cut(s)	460, 470, 721, 987, 1526, 1600, 2008, 2565
Hpy8I	GTNNAC	8 cut(s)	460, 470, 721, 987, 1526, 1600, 2008, 2565
Hpy99I	CGWCG	6 cut(s)	196, 199, 251, 254, 467, 2429
HpyAV	CCTTC	6 cut(s)	843, 876, 2126, 2752, 2817, 3044
HpyF10VI	GCNNNNNNNGC	8 cut(s)	86, 223, 356, 635, 1124, 1805, 2087, 2772
Hsp92I	GRCGYC	2 cut(s)	194, 2698
HspAI	GCGC	5 cut(s)	1085, 2067, 2360, 2362, 2698
KasI	GGCGCC	1 cut(s)	2697
Ksp22I	TGATCA	1 cut(s)	538
LmnI	GCTCC	9 cut(s)	296, 311, 650, 1435, 2087, 2236, 2851, 3059, 3130
Lsp1109I	GCAGC	6 cut(s)	80, 499, 928, 1139, 1277, 1393
LweI	GCATC	5 cut(s)	565, 1105, 1321, 1348, 1730
MaeI	CTAG	5 cut(s)	137, 455, 1610, 2217, 3035
MauBI	CGCGCGCG	1 cut(s)	2360
MbiI	CCGCTC	1 cut(s)	3014
MboII	GAAGA	8 cut(s)	277, 1630, 2089, 2771, 2870, 2977, 3037, 3040
MfeI	CAATTG	2 cut(s)	1274, 2537
MflI	RGATCY	4 cut(s)	2504, 2516, 2803, 2914
MhlI	GDGCHC	1 cut(s)	2943
MlsI	TGGCCA	1 cut(s)	1144
MluI	ACGCGT	1 cut(s)	965
MluNI	TGGCCA	1 cut(s)	1144
Mly113I	GGCGCC	1 cut(s)	2698
MmeI	TCCRAC	7 cut(s)	427, 640, 758, 1057, 2569, 3084, 3125
Mox20I	TGGCCA	1 cut(s)	1144
MroXI	GAANNNNTTC	1 cut(s)	2379
MscI	TGGCCA	1 cut(s)	1144
MseI	TTAA	9 cut(s)	59, 924, 1716, 1749, 2124, 2448, 2639, 2819, 3080
MslI	CAYNNNNRTG	5 cut(s)	530, 772, 1356, 1459, 2574
Msp20I	TGGCCA	1 cut(s)	1144
MspA1I	CMGCKG	2 cut(s)	941, 1727
MspCI	CTTAAG	1 cut(s)	1715
MspI	CCGG	3 cut(s)	210, 347, 914
MspR9I	CCNGG	6 cut(s)	211, 1141, 1586, 1935, 2043, 2849
MunI	CAATTG	2 cut(s)	1274, 2537
Mva1269I	GAATGC	3 cut(s)	27, 1192, 2616
MvaI	CCWGG	5 cut(s)	1141, 1586, 1935, 2043, 2849
MvnI	CGCG	7 cut(s)	921, 967, 2069, 2360, 2362, 2364, 2834
MwoI	GCNNNNNNNGC	8 cut(s)	86, 223, 356, 635, 1124, 1805, 2087, 2772
NarI	GGCGCC	1 cut(s)	2698
NciI	CCSGG	1 cut(s)	211
NmuCI	GTSAC	7 cut(s)	33, 583, 948, 1660, 2212, 2302, 2782
NruI	TCGCGA	1 cut(s)	2834
NspI	RCATGY	4 cut(s)	502, 777, 1464, 2243
NspV	TTCGAA	1 cut(s)	3139
PaeI	GCATGC	3 cut(s)	502, 1464, 2243
PaeR7I	CTCGAG	1 cut(s)	731
PagI	TCATGA	1 cut(s)	1354
PauI	GCGCGC	1 cut(s)	2360
PceI	AGGCCT	1 cut(s)	2081
PcsI	WCGNNNNNNNCGW	1 cut(s)	2901
PctI	GAATGC	3 cut(s)	27, 1192, 2616
PdmI	GAANNNNTTC	1 cut(s)	2379
Pfl23II	CGTACG	1 cut(s)	968
PflFI	GACNNNGTC	1 cut(s)	1471
PflMI	CCANNNNNTGG	3 cut(s)	73, 1823, 2854
PfoI	TCCNGGA	1 cut(s)	2847
PkrI	GCNGC	8 cut(s)	79, 95, 514, 943, 1129, 1253, 1267, 1383
PluTI	GGCGCC	1 cut(s)	2701
PmaCI	CACGTG	1 cut(s)	1387
PmlI	CACGTG	1 cut(s)	1387
Ppu21I	YACGTR	2 cut(s)	178, 1387
PpuMI	RGGWCCY	1 cut(s)	3089
PshBI	ATTAAT	2 cut(s)	59, 2448
Psp124BI	GAGCTC	1 cut(s)	2943
Psp1406I	AACGTT	1 cut(s)	406
Psp5II	RGGWCCY	1 cut(s)	3089
Psp6I	CCWGG	5 cut(s)	1139, 1584, 1933, 2041, 2847
PspCI	CACGTG	1 cut(s)	1387
PspGI	CCWGG	5 cut(s)	1139, 1584, 1933, 2041, 2847
PspLI	CGTACG	1 cut(s)	968
PspPI	GGNCC	6 cut(s)	232, 1307, 1931, 2162, 2264, 3089
PspPPI	RGGWCCY	1 cut(s)	3089
PspXI	VCTCGAGB	1 cut(s)	731
PstI	CTGCAG	1 cut(s)	631
PstNI	CAGNNNCTG	4 cut(s)	1487, 1562, 1811, 2854
PsuI	RGATCY	4 cut(s)	2504, 2516, 2803, 2914
PsyI	GACNNNGTC	1 cut(s)	1471
PteI	GCGCGC	1 cut(s)	2360
PvuII	CAGCTG	1 cut(s)	941
RruI	TCGCGA	1 cut(s)	2834
RseI	CAYNNNNRTG	5 cut(s)	530, 772, 1356, 1459, 2574
SacI	GAGCTC	1 cut(s)	2943
SaqAI	TTAA	9 cut(s)	59, 924, 1716, 1749, 2124, 2448, 2639, 2819, 3080
SatI	GCNGC	8 cut(s)	78, 94, 513, 942, 1128, 1252, 1266, 1382
Sau96I	GGNCC	6 cut(s)	232, 1307, 1931, 2162, 2264, 3089
ScaI	AGTACT	3 cut(s)	135, 281, 2657
ScrFI	CCNGG	6 cut(s)	211, 1141, 1586, 1935, 2043, 2849
SduI	GDGCHC	1 cut(s)	2943
SfaNI	GCATC	5 cut(s)	565, 1105, 1321, 1348, 1730
SfcI	CTRYAG	2 cut(s)	627, 2389
SfoI	GGCGCC	1 cut(s)	2699
Sfr274I	CTCGAG	1 cut(s)	731
SfuI	TTCGAA	1 cut(s)	3139
SinI	GGWCC	3 cut(s)	232, 2162, 3089
SlaI	CTCGAG	1 cut(s)	731
SmiMI	CAYNNNNRTG	5 cut(s)	530, 772, 1356, 1459, 2574
SmlI	CTYRAG	2 cut(s)	731, 1715
SmoI	CTYRAG	2 cut(s)	731, 1715
SpeI	ACTAGT	2 cut(s)	136, 3034
SphI	GCATGC	3 cut(s)	502, 1464, 2243
SseBI	AGGCCT	1 cut(s)	2081
SspDI	GGCGCC	1 cut(s)	2697
SspI	AATATT	1 cut(s)	1537
SspMI	CTAG	5 cut(s)	137, 455, 1610, 2217, 3035
SstI	GAGCTC	1 cut(s)	2943
StuI	AGGCCT	1 cut(s)	2081
StyD4I	CCNGG	6 cut(s)	209, 1139, 1584, 1933, 2041, 2847
TaqII	GACCGA	1 cut(s)	1169
TatI	WGTACW	7 cut(s)	133, 183, 279, 877, 2179, 2223, 2655
TauI	GCSGC	2 cut(s)	80, 1254
Tru1I	TTAA	9 cut(s)	59, 924, 1716, 1749, 2124, 2448, 2639, 2819, 3080
Tru9I	TTAA	9 cut(s)	59, 924, 1716, 1749, 2124, 2448, 2639, 2819, 3080
TscAI	CASTG	8 cut(s)	29, 636, 1117, 1251, 1526, 1657, 1669, 1999
TseFI	GTSAC	7 cut(s)	33, 583, 948, 1660, 2212, 2302, 2782
TseI	GCWGC	6 cut(s)	93, 512, 941, 1127, 1265, 1381
Tsp45I	GTSAC	7 cut(s)	33, 583, 948, 1660, 2212, 2302, 2782
TspGWI	ACGGA	5 cut(s)	21, 727, 1013, 2495, 2996
TspRI	CASTG	8 cut(s)	29, 636, 1117, 1251, 1526, 1657, 1669, 1999
Tth111I	GACNNNGTC	1 cut(s)	1471
Van91I	CCANNNNNTGG	3 cut(s)	73, 1823, 2854
Vha464I	CTTAAG	1 cut(s)	1715
VpaK11BI	GGWCC	3 cut(s)	232, 2162, 3089
VspI	ATTAAT	2 cut(s)	59, 2448
XapI	RAATTY	3 cut(s)	2252, 2379, 3135
XceI	RCATGY	4 cut(s)	502, 777, 1464, 2243
XhoI	CTCGAG	1 cut(s)	731
XmiI	GTMKAC	1 cut(s)	1599
XmnI	GAANNNNTTC	1 cut(s)	2379
XspI	CTAG	5 cut(s)	137, 455, 1610, 2217, 3035
ZraI	GACGTC	1 cut(s)	195
ZrmI	AGTACT	3 cut(s)	135, 281, 2657

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.