Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0001301

Physical Location & Seq

Genomic Coordinates Forward (+)

464171 .. 472168

Gene Structure 7998 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1007000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2988 bp

ATGGCCACGACTTGGTCCCTCTTGTGCATGCTTACGTTGGTCGTCGGATTTCTAGTCGCCGACTCCAAGTTTATTGCATACAACACTTCCCAAGGCATAGTCCCCGGCAAAATCAATGTCCATTTGGTCCCTCACACCCACGACGACGTTGGCTGGTTAAAGACCGTTGATCAGTATTATGTTGGCTCCAACAATTCCATCCAGGCATTCTTCCAGCGGTGGTGGAGAGATCAGAGTGACGCCGTCCAGAGTATAGTCAAGCAGCTAGTCACCTCGGGTCAATTAGAATTCATAAATGGGGGAATGTGTATGCATGATGAGGCTGCAACGCATTATATCGATATGATAGATCAGACGACTCTAGGGCATCGCTTTATTAAAAAGGAATTTGACATGACTCCAAGAATTGGTTGGCAAATTGACCCCTTTGGGCATTCGGCAGTCCAGGCTTATTTGTTGGGAGCAGAAGCGGGATTCGACTCACTTTTCTTTGGTCGAATAGATTACCAGGATAGGGCTAAGCGCAAAAATGACAAGAGCCTTGAATTTATTTGGCAGGGTTCGAAGAGTCTCGGTTCATCTGCTCAGATTTTTTCTGGTGCATTCCCTGAGGCTTATGAGCCTCCATCTGGTTTCTATTTTGAAGTTAATGATGATTCCCCAATTGTTCAGGATGACATCACTTTGTTTGATTACAATGTACATGATCGTGTTAATGACTTTGTTGCTACTGCAGTATCACAGGCTAACATAACTCGAACGAATCATATAATGTGGACCATGGGAACAGATTTCATGTATCAGTATGCACACACATGGTTCCGACAAATGGACAAACTCATTCACTATGTCAACATGGATGGACGTGTTAATGCTCTGTATTCTACTCCATCCATATATACGGATGCAAAGTATGCTACAAATGAATCATGGCCAATCAAGACCGGTGATTTCTTTCCTTATGCAGATCGTGCAAATGCATACTGGACAGGGTACTTCACAAGTAGACCAGCCTTGAAACATTACGTTAGAACAATGAGTGGCTATTATTTGGCTGCAAGGCAACTAGAATTTTTTATAGGAAGGACTGATTCAGGGCCCAACACAGATTCATTGGCAGATGCCTTGGCAATTGCTCAACATCATGATGCAGTCACTGGTACAGAGAAGCAGCATGTTGCCAATGATTATGCAAAAAGATTGGCCATAGGCTACACGGAGGCCGAGCAAGTAGTTGCCACGTCACTTGCTCACTTGGTTGAGCCTGCATCATACACTGGATCTGTAAATCCAACTACGAAGTTTCAACAGTGCCCACTTCTGAATATCAGCTATTGCCCTGCATCAGAAATGAATTTTTCTGAAGCGAAACAATTGATTGTCATGGTGTACAATTCCCTAGGATGGAAGAGGGATGATGTAATTCGAATTCCTATTATCAATGAAGATGTCACTGTTCATGATTCTGAGGGAAGAGAAATTGAATCACAGCTTCTCCCACTAGATGATGCCCATGTGGGCTTAAGGAACTACTATGCTAAGGCATACTTGGGTCAAACTCCAACAAAGACACCAAACTATTGGCTTGCGTTTACAGTATCCGTGCCACCACTCGGTTTCAACACATACACTATCTCGCTTTTGGGTAAAGGTGCTGGTTCCACCAGATCATCTGTACAGACATTTCAAAGTAGGGAAGAGTCCACTATTGAAGTTGGTCAAGGAAATGTGAAACTCACGTTTTCTACAAATCAAGGCAAAATGACTAAATATGTCAACAGAAGAAGCTTGGTTGAAGAATTGGTCGAACAGTCTTACGGTTTTTATAATGGATATAATGGCAGTGATGATAAAGCTCCTCTGATTCCTCAGAATGCCGGGGCATATATCTTCCGTCCAAATGGCACGTTTCTCATAAAACCTGGAGAAAAGGCTTCTCTGACTGTCGTGCGTGGACCAGTAATAGATGAAGTGCATCAACAAATTAATGCATGGATATATCAGGTCACCAGAATTCACAAAGAGAAGGAGCATGTTGAATTCGAGTTTATTGTTGGGCCATTACCTACTGATGACGGAATTGGCAAAGAAGTTGTAACTCAACTAGCCACTACCATGGCTACCAATAAAACGTTCTACACTGATTCTAATGGGCGTGATTTTATTGAAAGGATTTGCGACTATAGAACAGATTGGGACCTGAAAGTGCACCAACCTATAGCCGGAAATTATTATCCAATTAATCTTGGGATATACATGCAAGATAACAGTACAGAATTTTCGGTTTTGGTGGATCGATCTATAGGAGGGTCCAGCACAGTGGATGGACAGATAGAGCTGATGCTCCACAGGAGACTACTACTTGATGACTCAAGAGGTGTGGCAGAGGCTCTAAATGAAACTGTCTGTATTGACAATGTTTGCAGTGGACTAAGAATCCAAGGAAAATTTTATTTCAGAATCGACCCCCTGGGAGAAGGTGCCAAGTGGCGTCGCACTTTTGGTCAGGAGATATATTCCCCTCTTTTACTGGCCTTTTCTGAACAGGATGGAGATAACTGGAAGAATTCTCATGTAACCACTTTTTCAGGGGTTGGTTCCTCGTACAGTTTACCTAATAACGTCGCTCTTATAACTCTTCAGGAGCTTGATGATGGAAAAGTTCTCCTTCGATTAGCACATTTATATGAGATTGGAGAGGACAGGGATCTTTCTGTAATGGCAAATGTAGAGTTGAAGCAGCTCTTCCCCAGGAAGAAGATTGGTAAACTTACAGAGATGAACTTATCGGCAAATCAAGAACGGACAGAAATGGAGAAAAAGAGACTCAATTGGAAGGTGGAAGAAGGCTCTTCATCAGAAGCAAAGGTTGCGAGGGGTGGAACTGTTGATCCTGCAAAACTGGTTGTGGAACTTGCACCAATGGAAATCCGAACCTTTCTCGTTGAGTTTAACCAAAGCTTCAATCATTATTCATTTGATGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0001775 GO:0002252 GO:0002263 GO:0002274 GO:0002275 GO:0002283 GO:0002366 GO:0002376 GO:0002443 GO:0002444 GO:0002446 GO:0003674 GO:0003824 GO:0004553 GO:0004559 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005766 GO:0005773 GO:0005775 GO:0005975 GO:0005996 GO:0006013 GO:0006464 GO:0006517 GO:0006807 GO:0006810 GO:0006887 GO:0006955 GO:0008150 GO:0008152 GO:0009056 GO:0009100 GO:0009311 GO:0009313 GO:0009987 GO:0012505 GO:0015923 GO:0016052 GO:0016192 GO:0016787 GO:0016798 GO:0019318 GO:0019538 GO:0030141 GO:0031410 GO:0031974 GO:0031982 GO:0031983 GO:0032940 GO:0034774 GO:0035578 GO:0036211 GO:0036230 GO:0042119 GO:0042582 GO:0043170 GO:0043202 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043299 GO:0043312 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044281 GO:0044421 GO:0044422 GO:0044424 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045055 GO:0045321 GO:0046903 GO:0050896 GO:0051179 GO:0051234 GO:0060205 GO:0070013 GO:0071704 GO:0097708 GO:0099503 GO:1901135 GO:1901564 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Alpha-mann_mid Glyco_hydro_38 Glyco_hydro_38C

Protein Analysis

996

Amino Acids

112.23

Weight (kDa)

5.47

Isoelectric Point (pI)

37.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	39 - 66	4.1e-06	Glycosyl hydrolases family 38 N-terminal domain
Glyco_hydro_38N	PF01074	68 - 322	8.5e-61	Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid	PF09261	327 - 419	2e-21	Alpha mannosidase middle domain
Laman-like_dom	PF21260	494 - 545	9.8e-10	Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C	PF07748	573 - 792	6.8e-46	Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2	PF17677	874 - 980	3.9e-08	Glycosyl hydrolases family 38 C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001301)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13980 AT5G13980 AT5G13980
fragaria_vesca	FvH4_5g09420 FvH4_5g09421 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272
malus_domestica	MD06G1131600.v1.1 MD09G1002900.v1.1 MD17G1007000.v1.1
prunus_persica	Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.5G142600_v2.0.a1 Prupe.5G142600_v2.0.a1
pyrus_communis	pycom06g12300 pycom06g12310 pycom111g00240 pycom17g00260
rosa_chinensis	RchiOBHm_Chr2g0176471 RchiOBHm_Chr2g0176481 RchiOBHm_Chr7g0188771 RchiOBHm_Chr7g0188781
rosa_laevigata	RLG00000004661 RLG00000004662 RLG00000022416
rosa_multiflora	Rmu_sc0000303.1_g000057 Rmu_sc0003642.1_g000008 Rmu_sc0011904.1_g000012
rosa_roxburghii	Rroxscaffold_2G00076370 Rroxscaffold_3G00266040 Rroxscaffold_3G00266050
rosa_rugosa	Rorug02G0595000 Rorug06G0493700
rosa_samantha	Rh2AG673300 Rh2BG684600 Rh2CG647400 Rh2DG698200 Rh7AG098300 Rh7AG098400 Rh7BG099900 Rh7BG100200 Rh7CG101600 Rh7CG101700 Rh7DG100100 Rh7DG100200
rosa_wichuraiana	Rw0G010390 Rw2G055220 Rw7G008480 Rw7G008490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1827, 2633
AccB1I	GGYRCC	1 cut(s)	2480
AccB7I	CCANNNNNTGG	2 cut(s)	12, 407
AccI	GTMKAC	1 cut(s)	1006
AciI	CCGC	2 cut(s)	217, 470
AclI	AACGTT	1 cut(s)	2132
AclWI	GGATC	4 cut(s)	1288, 2301, 2715, 2885
AcoI	YGGCCR	3 cut(s)	3, 932, 1203
AcuI	CTGAAG	2 cut(s)	1383, 2624
AcyI	GRCGYC	2 cut(s)	240, 2491
AfaI	GTAC	7 cut(s)	702, 995, 1162, 1391, 1677, 2272, 2606
AfiI	CCNNNNNNNGG	8 cut(s)	12, 407, 514, 629, 829, 1404, 1613, 2222
AflII	CTTAAG	1 cut(s)	1522
AflIII	ACRYGT	1 cut(s)	865
AgeI	ACCGGT	1 cut(s)	944
AjiI	CACGTC	2 cut(s)	866, 1242
AjnI	CCWGG	6 cut(s)	201, 444, 507, 1921, 2469, 2750
AjuI	GAANNNNNNNTTGG	2 cut(s)	2117, 2149
AloI	GAACNNNNNNTCC	2 cut(s)	2875, 2907
AluBI	AGCT	9 cut(s)	265, 1332, 1492, 1788, 1856, 2338, 2647, 2743, 2961
AluI	AGCT	9 cut(s)	265, 1332, 1492, 1788, 1856, 2338, 2647, 2743, 2961
Alw21I	GWGCWC	1 cut(s)	2211
Alw26I	GTCTC	3 cut(s)	575, 2347, 2818
Alw44I	GTGCAC	1 cut(s)	2207
AlwI	GGATC	4 cut(s)	1288, 2301, 2715, 2885
AlwNI	CAGNNNCTG	1 cut(s)	1157
Ama87I	CYCGRG	1 cut(s)	274
AoxI	GGCC	7 cut(s)	3, 932, 1097, 1203, 1221, 2057, 2532
ApaI	GGGCCC	1 cut(s)	1101
ApaLI	GTGCAC	1 cut(s)	2207
ApeKI	GCWGC	5 cut(s)	262, 323, 1055, 1171, 2740
ArsI	GACNNNNNNTTYG	2 cut(s)	102, 134
AseI	ATTAAT	2 cut(s)	1986, 2241
AsiGI	ACCGGT	1 cut(s)	944
AspA2I	CCTAGG	1 cut(s)	1399
AspLEI	GCGC	1 cut(s)	525
AspS9I	GGNCC	9 cut(s)	15, 127, 777, 1097, 1098, 1955, 2057, 2197, 2310
AsuC2I	CCSGG	2 cut(s)	105, 1879
AsuHPI	GGTGA	3 cut(s)	262, 959, 1999
AsuII	TTCGAA	2 cut(s)	563, 1426
AvaI	CYCGRG	1 cut(s)	274
AvaII	GGWCC	6 cut(s)	15, 127, 777, 1955, 2197, 2310
AvrII	CCTAGG	1 cut(s)	1399
AxyI	CCTNAGG	1 cut(s)	609
BaeGI	GKGCMC	3 cut(s)	1101, 1316, 2211
BaeI	ACNNNNGTAYC	2 cut(s)	720, 753
BalI	TGGCCA	3 cut(s)	5, 934, 1205
BanI	GGYRCC	1 cut(s)	2480
BanII	GRGCYC	1 cut(s)	1101
Bbv12I	GWGCWC	1 cut(s)	2211
BbvI	GCAGC	5 cut(s)	274, 310, 1042, 1183, 2752
BccI	CCATC	8 cut(s)	206, 634, 854, 898, 1398, 2318, 2543, 2648
BceAI	ACGGC	1 cut(s)	227
BciT130I	CCWGG	6 cut(s)	203, 446, 509, 1923, 2471, 2752
BciVI	GTATCC	1 cut(s)	1609
BclI	TGATCA	1 cut(s)	169
BcnI	CCSGG	2 cut(s)	105, 1879
BcoDI	GTCTC	3 cut(s)	575, 2347, 2818
BfaI	CTAG	7 cut(s)	53, 266, 362, 1067, 1400, 1502, 2105
BfmI	CTRYAG	4 cut(s)	732, 2182, 2217, 2301
BfrI	CTTAAG	1 cut(s)	1522
BfuI	GTATCC	1 cut(s)	1609
BisI	GCNGC	5 cut(s)	263, 324, 1056, 1172, 2741
BlnI	CCTAGG	1 cut(s)	1399
BlpI	GCTNAGC	1 cut(s)	519
BlsI	GCNGC	5 cut(s)	264, 325, 1057, 1173, 2742
Bme1390I	CCNGG	8 cut(s)	105, 203, 446, 509, 1879, 1923, 2471, 2752
Bme18I	GGWCC	6 cut(s)	15, 127, 777, 1955, 2197, 2310
BmeT110I	CYCGRG	1 cut(s)	274
BmgBI	CACGTC	2 cut(s)	866, 1242
BmgT120I	GGNCC	9 cut(s)	15, 127, 777, 1097, 1098, 1955, 2057, 2197, 2310
BmrFI	CCNGG	8 cut(s)	105, 203, 446, 509, 1879, 1923, 2471, 2752
BpmI	CTGGAG	1 cut(s)	1944
Bpu10I	CCTNAGC	1 cut(s)	1539
Bpu1102I	GCTNAGC	1 cut(s)	519
Bpu14I	TTCGAA	2 cut(s)	563, 1426
BpuEI	CTTGAG	1 cut(s)	2356
BpuMI	CCSGG	2 cut(s)	105, 1879
Bsa29I	ATCGAT	2 cut(s)	339, 2296
BsaHI	GRCGYC	2 cut(s)	240, 2491
BsaWI	WCCGGW	1 cut(s)	944
BsaXI	ACNNNNNCTCC	2 cut(s)	1479, 1509
Bsc4I	CCNNNNNNNGG	8 cut(s)	12, 407, 514, 629, 829, 1404, 1613, 2222
Bse118I	RCCGGY	1 cut(s)	944
Bse1I	ACTGG	7 cut(s)	989, 1162, 1282, 1958, 2535, 2564, 2907
Bse21I	CCTNAGG	1 cut(s)	609
BseBI	CCWGG	6 cut(s)	203, 446, 509, 1923, 2471, 2752
BseCI	ATCGAT	2 cut(s)	339, 2296
BseGI	GGATG	9 cut(s)	198, 679, 865, 890, 910, 1409, 1420, 2329, 2554
BseLI	CCNNNNNNNGG	8 cut(s)	12, 407, 514, 629, 829, 1404, 1613, 2222
BseMII	CTCAG	4 cut(s)	599, 600, 1458, 1883
BseNI	ACTGG	7 cut(s)	989, 1162, 1282, 1958, 2535, 2564, 2907
BseRI	GAGGAG	1 cut(s)	1848
BseSI	GKGCMC	3 cut(s)	1101, 1316, 2211
BseXI	GCAGC	5 cut(s)	274, 310, 1042, 1183, 2752
BshFI	GGCC	7 cut(s)	5, 934, 1099, 1205, 1223, 2059, 2534
BshNI	GGYRCC	1 cut(s)	2480
BshTI	ACCGGT	1 cut(s)	944
BshVI	ATCGAT	2 cut(s)	339, 2296
BsiHKAI	GWGCWC	1 cut(s)	2211
BsiHKCI	CYCGRG	1 cut(s)	274
BsiSI	CCGG	4 cut(s)	105, 945, 1878, 2223
BslFI	GGGAC	3 cut(s)	86, 113, 2210
BslI	CCNNNNNNNGG	8 cut(s)	12, 407, 514, 629, 829, 1404, 1613, 2222
BsmAI	GTCTC	3 cut(s)	575, 2347, 2818
BsmFI	GGGAC	3 cut(s)	86, 113, 2210
BsmI	GAATGC	4 cut(s)	206, 433, 602, 1879
BsnI	GGCC	7 cut(s)	5, 934, 1099, 1205, 1223, 2059, 2534
BsoBI	CYCGRG	1 cut(s)	274
Bsp119I	TTCGAA	2 cut(s)	563, 1426
Bsp120I	GGGCCC	1 cut(s)	1097
Bsp1286I	GDGCHC	3 cut(s)	1101, 1316, 2211
Bsp1407I	TGTACA	3 cut(s)	700, 1389, 1675
Bsp1720I	GCTNAGC	1 cut(s)	519
Bsp19I	CCATGG	2 cut(s)	780, 2115
BspACI	CCGC	2 cut(s)	217, 470
BspANI	GGCC	7 cut(s)	5, 934, 1099, 1205, 1223, 2059, 2534
BspCNI	CTCAG	4 cut(s)	598, 601, 1459, 1882
BspDI	ATCGAT	2 cut(s)	339, 2296
BspHI	TCATGA	2 cut(s)	1144, 1459
BspMAI	CTGCAG	1 cut(s)	736
BspPI	GGATC	4 cut(s)	1288, 2301, 2715, 2885
BspQI	GCTCTTC	2 cut(s)	2750, 2857
BspT104I	TTCGAA	2 cut(s)	563, 1426
BspT107I	GGYRCC	1 cut(s)	2480
BspTI	CTTAAG	1 cut(s)	1522
BsrFI	RCCGGY	1 cut(s)	944
BsrGI	TGTACA	3 cut(s)	700, 1389, 1675
BsrI	ACTGG	7 cut(s)	989, 1162, 1282, 1958, 2535, 2564, 2907
BssAI	RCCGGY	1 cut(s)	944
BssNI	GRCGYC	2 cut(s)	240, 2491
BssT1I	CCWWGG	6 cut(s)	91, 780, 1125, 1399, 2115, 2440
Bst2UI	CCWGG	6 cut(s)	203, 446, 509, 1923, 2471, 2752
Bst6I	CTCTTC	7 cut(s)	560, 1403, 1468, 1692, 2643, 2750, 2857
BstACI	GRCGYC	2 cut(s)	240, 2491
BstAFI	CTTAAG	1 cut(s)	1522
BstAPI	GCANNNNNTGC	3 cut(s)	914, 971, 2870
BstAUI	TGTACA	3 cut(s)	700, 1389, 1675
BstBI	TTCGAA	2 cut(s)	563, 1426
BstC8I	GCNNGC	3 cut(s)	29, 1266, 1587
BstDEI	CTNAG	7 cut(s)	519, 585, 609, 1467, 1539, 1869, 2432
BstDSI	CCRYGG	2 cut(s)	780, 2115
BstEII	GGTNACC	1 cut(s)	2005
BstF5I	GGATG	9 cut(s)	198, 679, 865, 890, 910, 1409, 1420, 2329, 2554
BstHHI	GCGC	1 cut(s)	525
BstMAI	GTCTC	3 cut(s)	575, 2347, 2818
BstMWI	GCNNNNNNNGC	4 cut(s)	446, 914, 971, 2870
BstNI	CCWGG	6 cut(s)	203, 446, 509, 1923, 2471, 2752
BstNSI	RCATGY	4 cut(s)	31, 1178, 2036, 2260
BstPI	GGTNACC	1 cut(s)	2005
BstSCI	CCNGG	8 cut(s)	103, 201, 444, 507, 1877, 1921, 2469, 2750
BstSFI	CTRYAG	4 cut(s)	732, 2182, 2217, 2301
BstSLI	GKGCMC	3 cut(s)	1101, 1316, 2211
BstV1I	GCAGC	5 cut(s)	274, 310, 1042, 1183, 2752
BstX2I	RGATCY	2 cut(s)	1280, 2707
BstXI	CCANNNNNNTGG	3 cut(s)	1581, 2116, 2320
BstYI	RGATCY	2 cut(s)	1280, 2707
Bsu15I	ATCGAT	2 cut(s)	339, 2296
Bsu36I	CCTNAGG	1 cut(s)	609
BsuI	GTATCC	1 cut(s)	1609
BsuRI	GGCC	7 cut(s)	5, 934, 1099, 1205, 1223, 2059, 2534
BsuTUI	ATCGAT	2 cut(s)	339, 2296
BtgI	CCRYGG	2 cut(s)	780, 2115
BtgZI	GCGATG	1 cut(s)	353
BtrI	CACGTC	2 cut(s)	866, 1242
BtsCI	GGATG	9 cut(s)	198, 679, 865, 890, 910, 1409, 1420, 2329, 2554
BtsI	GCAGTG	2 cut(s)	1849, 2431
BtsIMutI	CAGTG	8 cut(s)	1155, 1275, 1316, 1452, 1849, 2139, 2325, 2431
Cac8I	GCNNGC	3 cut(s)	29, 1266, 1587
CaiI	CAGNNNCTG	1 cut(s)	1157
CciI	TCATGA	2 cut(s)	1144, 1459
CfoI	GCGC	1 cut(s)	525
Cfr10I	RCCGGY	1 cut(s)	944
Cfr13I	GGNCC	9 cut(s)	15, 127, 777, 1097, 1098, 1955, 2057, 2197, 2310
ClaI	ATCGAT	2 cut(s)	339, 2296
CseI	GACGC	2 cut(s)	248, 2480
Csp6I	GTAC	7 cut(s)	701, 994, 1161, 1390, 1676, 2271, 2605
CspAI	ACCGGT	1 cut(s)	944
CspCI	CAANNNNNGTGG	4 cut(s)	1228, 1263, 2361, 2396
CviQI	GTAC	7 cut(s)	701, 994, 1161, 1390, 1676, 2271, 2605
DdeI	CTNAG	7 cut(s)	519, 585, 609, 1467, 1539, 1869, 2432
EaeI	YGGCCR	3 cut(s)	3, 932, 1203
Eam1104I	CTCTTC	7 cut(s)	560, 1403, 1468, 1692, 2643, 2750, 2857
EarI	CTCTTC	7 cut(s)	560, 1403, 1468, 1692, 2643, 2750, 2857
Eco130I	CCWWGG	6 cut(s)	91, 780, 1125, 1399, 2115, 2440
Eco24I	GRGCYC	1 cut(s)	1101
Eco47I	GGWCC	6 cut(s)	15, 127, 777, 1955, 2197, 2310
Eco57I	CTGAAG	2 cut(s)	1383, 2624
Eco81I	CCTNAGG	1 cut(s)	609
Eco88I	CYCGRG	1 cut(s)	274
Eco91I	GGTNACC	1 cut(s)	2005
EcoO109I	RGGNCCY	2 cut(s)	1097, 2197
EcoO65I	GGTNACC	1 cut(s)	2005
EcoRI	GAATTC	5 cut(s)	287, 1428, 2013, 2039, 2566
EcoRII	CCWGG	6 cut(s)	201, 444, 507, 1921, 2469, 2750
EcoT14I	CCWWGG	6 cut(s)	91, 780, 1125, 1399, 2115, 2440
EcoT22I	ATGCAT	3 cut(s)	315, 982, 1993
EcoT38I	GRGCYC	1 cut(s)	1101
ErhI	CCWWGG	6 cut(s)	91, 780, 1125, 1399, 2115, 2440
FalI	AAGNNNNNCTT	6 cut(s)	1532, 1564, 2652, 2684, 2729, 2761
FaqI	GGGAC	3 cut(s)	86, 113, 2210
FauI	CCCGC	1 cut(s)	463
FbaI	TGATCA	1 cut(s)	169
FblI	GTMKAC	1 cut(s)	1006
Fnu4HI	GCNGC	5 cut(s)	263, 324, 1056, 1172, 2741
FokI	GGATG	9 cut(s)	185, 686, 872, 877, 917, 1416, 1427, 2336, 2561
FriOI	GRGCYC	1 cut(s)	1101
Fsp4HI	GCNGC	5 cut(s)	263, 324, 1056, 1172, 2741
FspBI	CTAG	7 cut(s)	53, 266, 362, 1067, 1400, 1502, 2105
GlaI	GCGC	1 cut(s)	524
GluI	GCNGC	5 cut(s)	263, 324, 1056, 1172, 2741
GsuI	CTGGAG	1 cut(s)	1944
HaeIII	GGCC	7 cut(s)	5, 934, 1099, 1205, 1223, 2059, 2534
HapII	CCGG	4 cut(s)	105, 945, 1878, 2223
HgaI	GACGC	2 cut(s)	248, 2480
HhaI	GCGC	1 cut(s)	525
Hin1I	GRCGYC	2 cut(s)	240, 2491
Hin6I	GCGC	1 cut(s)	523
HinP1I	GCGC	1 cut(s)	523
HincII	GTYRAC	2 cut(s)	853, 1777
HindII	GTYRAC	2 cut(s)	853, 1777
HindIII	AAGCTT	2 cut(s)	1786, 2959
HpaII	CCGG	4 cut(s)	105, 945, 1878, 2223
HphI	GGTGA	3 cut(s)	262, 959, 1999
Hpy188III	TCNNGA	9 cut(s)	247, 671, 940, 1145, 1460, 2373, 2507, 2642, 2798
Hpy99I	CGWCG	5 cut(s)	47, 146, 149, 2496, 2627
HpyAV	CCTTC	6 cut(s)	1077, 2020, 2471, 2678, 2830, 2840
HpyCH4IV	ACGT	9 cut(s)	35, 147, 865, 1026, 1241, 1739, 1907, 2132, 2622
HpyF10VI	GCNNNNNNNGC	4 cut(s)	446, 914, 971, 2870
HpyF3I	CTNAG	7 cut(s)	519, 585, 609, 1467, 1539, 1869, 2432
HpySE526I	ACGT	9 cut(s)	35, 147, 865, 1026, 1241, 1739, 1907, 2132, 2622
Hsp92I	GRCGYC	2 cut(s)	240, 2491
HspAI	GCGC	1 cut(s)	523
Ksp22I	TGATCA	1 cut(s)	169
LguI	GCTCTTC	2 cut(s)	2750, 2857
LmnI	GCTCC	6 cut(s)	191, 461, 1861, 2029, 2349, 2644
Lsp1109I	GCAGC	5 cut(s)	274, 310, 1042, 1183, 2752
MaeI	CTAG	7 cut(s)	53, 266, 362, 1067, 1400, 1502, 2105
MaeII	ACGT	9 cut(s)	35, 147, 865, 1026, 1241, 1739, 1907, 2132, 2622
MaeIII	GTNAC	8 cut(s)	236, 268, 1153, 1242, 1450, 2005, 2095, 2575
MfeI	CAATTG	4 cut(s)	663, 1131, 1373, 2830
MflI	RGATCY	2 cut(s)	1280, 2707
MhlI	GDGCHC	3 cut(s)	1101, 1316, 2211
MlsI	TGGCCA	3 cut(s)	5, 934, 1205
MluNI	TGGCCA	3 cut(s)	5, 934, 1205
MlyI	GAGTC	8 cut(s)	56, 352, 391, 473, 577, 1709, 2363, 2820
MmeI	TCCRAC	5 cut(s)	25, 213, 847, 1316, 1586
Mox20I	TGGCCA	3 cut(s)	5, 934, 1205
Mph1103I	ATGCAT	3 cut(s)	315, 982, 1993
MscI	TGGCCA	3 cut(s)	5, 934, 1205
MseI	TTAA	9 cut(s)	158, 378, 648, 714, 870, 1523, 1986, 2241, 2952
MslI	CAYNNNNRTG	5 cut(s)	708, 814, 1146, 2114, 2685
Msp20I	TGGCCA	3 cut(s)	5, 934, 1205
MspA1I	CMGCKG	1 cut(s)	217
MspCI	CTTAAG	1 cut(s)	1522
MspI	CCGG	4 cut(s)	105, 945, 1878, 2223
MspR9I	CCNGG	8 cut(s)	105, 203, 446, 509, 1879, 1923, 2471, 2752
MunI	CAATTG	4 cut(s)	663, 1131, 1373, 2830
Mva1269I	GAATGC	4 cut(s)	206, 433, 602, 1879
MvaI	CCWGG	6 cut(s)	203, 446, 509, 1923, 2471, 2752
MwoI	GCNNNNNNNGC	4 cut(s)	446, 914, 971, 2870
NciI	CCSGG	2 cut(s)	105, 1879
NcoI	CCATGG	2 cut(s)	780, 2115
NmeAIII	GCCGAG	1 cut(s)	1249
NmuCI	GTSAC	6 cut(s)	236, 268, 1153, 1242, 1450, 2005
NsiI	ATGCAT	3 cut(s)	315, 982, 1993
NspI	RCATGY	4 cut(s)	31, 1178, 2036, 2260
NspV	TTCGAA	2 cut(s)	563, 1426
PaeI	GCATGC	1 cut(s)	31
PagI	TCATGA	2 cut(s)	1144, 1459
PasI	CCCWGGG	1 cut(s)	2470
PciSI	GCTCTTC	2 cut(s)	2750, 2857
PctI	GAATGC	4 cut(s)	206, 433, 602, 1879
PflFI	GACNNNGTC	2 cut(s)	13, 242
PflMI	CCANNNNNTGG	2 cut(s)	12, 407
PinAI	ACCGGT	1 cut(s)	944
PkrI	GCNGC	5 cut(s)	264, 325, 1057, 1173, 2742
PleI	GAGTC	8 cut(s)	56, 352, 391, 473, 576, 1708, 2363, 2820
PpsI	GAGTC	8 cut(s)	56, 352, 391, 473, 576, 1708, 2363, 2820
PpuMI	RGGWCCY	1 cut(s)	2197
PshBI	ATTAAT	2 cut(s)	1986, 2241
PsiI	TTATAA	2 cut(s)	1827, 2633
Psp1406I	AACGTT	1 cut(s)	2132
Psp5II	RGGWCCY	1 cut(s)	2197
Psp6I	CCWGG	6 cut(s)	201, 444, 507, 1921, 2469, 2750
PspEI	GGTNACC	1 cut(s)	2005
PspGI	CCWGG	6 cut(s)	201, 444, 507, 1921, 2469, 2750
PspOMI	GGGCCC	1 cut(s)	1097
PspPI	GGNCC	9 cut(s)	15, 127, 777, 1097, 1098, 1955, 2057, 2197, 2310
PspPPI	RGGWCCY	1 cut(s)	2197
PstI	CTGCAG	1 cut(s)	736
PstNI	CAGNNNCTG	1 cut(s)	1157
PsuI	RGATCY	2 cut(s)	1280, 2707
PsyI	GACNNNGTC	2 cut(s)	13, 242
RsaI	GTAC	7 cut(s)	702, 995, 1162, 1391, 1677, 2272, 2606
RsaNI	GTAC	7 cut(s)	701, 994, 1161, 1390, 1676, 2271, 2605
RseI	CAYNNNNRTG	5 cut(s)	708, 814, 1146, 2114, 2685
SapI	GCTCTTC	2 cut(s)	2750, 2857
SaqAI	TTAA	9 cut(s)	158, 378, 648, 714, 870, 1523, 1986, 2241, 2952
SatI	GCNGC	5 cut(s)	263, 324, 1056, 1172, 2741
Sau96I	GGNCC	9 cut(s)	15, 127, 777, 1097, 1098, 1955, 2057, 2197, 2310
SchI	GAGTC	8 cut(s)	56, 352, 391, 473, 577, 1709, 2363, 2820
ScrFI	CCNGG	8 cut(s)	105, 203, 446, 509, 1879, 1923, 2471, 2752
SduI	GDGCHC	3 cut(s)	1101, 1316, 2211
SfcI	CTRYAG	4 cut(s)	732, 2182, 2217, 2301
SfuI	TTCGAA	2 cut(s)	563, 1426
SinI	GGWCC	6 cut(s)	15, 127, 777, 1955, 2197, 2310
SmiMI	CAYNNNNRTG	5 cut(s)	708, 814, 1146, 2114, 2685
SmlI	CTYRAG	2 cut(s)	1522, 2371
SmoI	CTYRAG	2 cut(s)	1522, 2371
SphI	GCATGC	1 cut(s)	31
SsiI	CCGC	2 cut(s)	217, 470
SspMI	CTAG	7 cut(s)	53, 266, 362, 1067, 1400, 1502, 2105
StyD4I	CCNGG	8 cut(s)	103, 201, 444, 507, 1877, 1921, 2469, 2750
StyI	CCWWGG	6 cut(s)	91, 780, 1125, 1399, 2115, 2440
TaiI	ACGT	9 cut(s)	38, 150, 868, 1029, 1244, 1742, 1910, 2135, 2625
TatI	WGTACW	4 cut(s)	700, 1389, 1675, 2270
Tru1I	TTAA	9 cut(s)	158, 378, 648, 714, 870, 1523, 1986, 2241, 2952
Tru9I	TTAA	9 cut(s)	158, 378, 648, 714, 870, 1523, 1986, 2241, 2952
TscAI	CASTG	8 cut(s)	1162, 1282, 1316, 1459, 1849, 2146, 2325, 2431
TseFI	GTSAC	6 cut(s)	236, 268, 1153, 1242, 1450, 2005
TseI	GCWGC	5 cut(s)	262, 323, 1055, 1171, 2740
Tsp45I	GTSAC	6 cut(s)	236, 268, 1153, 1242, 1450, 2005
TspGWI	ACGGA	6 cut(s)	917, 1232, 1591, 1883, 2091, 2818
TspRI	CASTG	8 cut(s)	1162, 1282, 1316, 1459, 1849, 2146, 2325, 2431
Tth111I	GACNNNGTC	2 cut(s)	13, 242
Van91I	CCANNNNNTGG	2 cut(s)	12, 407
Vha464I	CTTAAG	1 cut(s)	1522
VneI	GTGCAC	1 cut(s)	2207
VpaK11BI	GGWCC	6 cut(s)	15, 127, 777, 1955, 2197, 2310
VspI	ATTAAT	2 cut(s)	1986, 2241
XceI	RCATGY	4 cut(s)	31, 1178, 2036, 2260
XcmI	CCANNNNNNNNNTGG	2 cut(s)	146, 408
XmaJI	CCTAGG	1 cut(s)	1399
XmiI	GTMKAC	1 cut(s)	1006
XspI	CTAG	7 cut(s)	53, 266, 362, 1067, 1400, 1502, 2105
Zsp2I	ATGCAT	3 cut(s)	315, 982, 1993

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.