Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Contig00444

Orthogroup ID

OG0001301

Physical Location & Seq

Genomic Coordinates Forward (+)

4169 .. 11124

Gene Structure 6956 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw0G010390.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2838 bp

ATGCAAAGGAACTCTGTCCATTCTCATAATGTGTTTTTCCACATGAAAACCATTGGCTCTTATAATAAGTTCGAATTAAGCAACCTTAATTGTAAAAACTTACTTGATAATTTAAAACTTTGTTTCACGATTCTTTATACACGCGCCGCGGGCATAATTCCTAGTAATGACAAAATATGGGCGAGAGAACAGAGCGAGGCGATCCAAGACACAGTCAAGCAGCTAGTCGGCTCTGGCCAACTGGAATTCATAAATGGGGGGATGTGCATGCAAGATGAGGCTGCTACGCATTACATTGATATGATTGATCAGACGACTCTAGGGCATCGATTTATTAAGCAAGAGTTCAATGTGACTCCAAGAGTTGGCTGGCAAATCGATTCGTTTGGACATTCTTCTGTGCAGGCTTACCTTTTGGCGGCTGAGGTTGGCTTTGATTCCCTATTTTTTGGACGAATCGACTACCAAGATATAGCTACGAGGAAAAATGAGAAAAGCCTTGAGGTTATTTTTGCTGGTGCATTGCCTACGCTATATGTTCCTCCTTCTCATAATTTCTACTTTGACACTGATGACGTCCTTGTCCCTCTTGTACAAGATGATATGAATCTTGGTGACTACAACGTGGCAGATCGCGTTAATGATTTTGTATCTGAAGCAATATCACAAGCAAACATTACTCGTACAAATCATATCATGTGGACCATGGGAAAGGATTTCGCGTATCAATATGCTAATTCATGGTTCAGTCAGATGGACAAATTCGTTCATCATGTGAATCAAGATGGGCGTGTCAATGCCCTATACTCAACCCCATCAATATACACCGATGCGAAACATGCAACAAATGAGTCCTGGCCAATCAAGAGTGATGACTTCTTTCCATATGCTAGTGAAGTGAATGCATACTGGACTGGATATTTTACAAGCAGGCCAGCCCTCAAACGTTATGTTAGAATGATGAGTAGCTACTATTCGGCAGCAAGGCAATTGGAATTTTTTACGGGAATAAGTAAATCAGGGCCTAACACAGATTCTCTGGCTGATGCTTTAGCAATTGCTCAACATCATGATGCAGTTAGTGGCAGATCTAGGCAGCACGTAGCTGATGATTATACAAAACGACTTTCAATAGGTTACAAGGAGGCTGAGAAGGTTGTTGCAGAATCACTTTCTTGCTTGATAAGTTCAAGATTAAGCACTGGTTGCAAGAGTGTAGTAACAAAGTTTCAACAGTGTCCACTTCTGAATATTAGCTATTGCCCCCCGTCAGAAGTTGATCTTTCAAAGGGAAAACACCTGATTCCTGTTGTCAGTGAAAAAGTCACTGTAAAGGACTCCACCGGAAAGGAAATTGAATCACAGATTCTACCGCTGCTTAATGCATCTGTCAGCATAAGAAATGACCATGTTAAGGCATATTTGGGTATAGCACCGAGTGTGGCCACAAGTTATTGGCTTGCATTTTCAGCAACTGTACCACCTCTTGGTTTTAGCACTTACATGGTCTCAAGTGCTGCCACACAGACAGCTACTACTTCAGAGAGACATACAGTGTACAAGACAGAAACAAGTCAAAATGAAACAATAAAAGTTGGGCCAGGAAACTTGAAACTCATTTATTCTGGAAACGATGGAAAACTGATTCAGTACACAAACAGCAGAAGCTCGGTCAAAGAGTTGGTAATGCAATCATTTAGCTACTACCCTGGAGATGATGGAAGTGTGAAGGTTTTCGGTGAGGATGGAAATCTGTTCTCAGGAGCATATGTCTTCCGTCCTAATGGTACATATCCCATTAAATCCGAAGGGCAGGTTTCTTTGACTATTTTAAGGGGACCATTGTTGGATGAAATACATGAAAGGATCAACTCTTGGATATATCAGGTCACTAGAGTGTACAGGGAAAAAGAGCATGCTGAGATTGAATTTACTATAGGGCCTATACCAGTTGATGATGGAATAGGCAAAGATTTTGTAACTAAGATTACAACCAGCATGAGAACCAACAAACAATTCTACACAGATTCTAACGGGCGCGATTTTATTCAAAGAATTCGAGACTACAGAAAAGACTGGGACCTGGAAGTAAACCAACCTGTCGCAGGAAACTATTATCCGATTAATCTGGGAATCTATACGAAAGATAACAACACAGAGCTGTCAGTATTAGTGGATAGATCTGTAGGGGGATCCAGCATTGTGGACGGACAATTGGAACTGATGCTTCGTAGGAGATTGCTTGTAGGTGATGACAATGGTATTACAGAACAACTAAATGAAACGGTGTGCATTGAACATGATTGCAAAGGACTAACAATTACAGGAAAGTACCACCTGAGAATTGATCCTTTAGGAGAGGGTGCTAAGTGGCGTCGATCATTTGGTCAGGAGATATATTCTCCACTTCTGTTAGCCTTCACAGAACAAAATGGAGATGACTGGACAAACTCTCATGTCACAACCTTTTCGGGAATGGATCCTTCCTACACTTTACCTGATAATGTTGCAATTATAACTCTCCAGGAGCTGGAAGATGGGAAAGTTCTCTTTCGCCTGGCGCATTTATACGAGATTGAAGAGGACAAGCATCTTTCAGTTATGGCAAGTGTGGAACTGAAAAAGGTGTTTGCAAACAAGAAGATAAAGCATATAACCGAAATGAGCTTATCTGCTAACCAAGAAAGAAGGGAATTGGAAAGGAAGAGACTGGTTTGGAATGTGCAAGGCTCTTCTGAGGAAGGACCGAAAGGCTTGAGGGGACGACCTGTTGATCCAACAAAGCTAGTGCTGAATCTTGCTCCAATGGAAATTCGAACATTTACCATCGACTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Alpha-mann_mid Glyco_hydro_38 Glyco_hydro_38C

Protein Analysis

945

Amino Acids

106.52

Weight (kDa)

5.91

Isoelectric Point (pI)

35.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	60 - 297	1.9e-49	Glycosyl hydrolases family 38 N-terminal domain
Alpha-mann_mid	PF09261	302 - 394	1.3e-21	Alpha mannosidase middle domain
Laman-like_dom	PF21260	480 - 503	1.5e-08	Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C	PF07748	534 - 749	7e-39	Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2	PF17677	835 - 941	1.9e-07	Glycosyl hydrolases family 38 C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001301)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13980 AT5G13980 AT5G13980
fragaria_vesca	FvH4_5g09420 FvH4_5g09421 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272
malus_domestica	MD06G1131600.v1.1 MD09G1002900.v1.1 MD17G1007000.v1.1
prunus_persica	Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.5G142600_v2.0.a1 Prupe.5G142600_v2.0.a1
pyrus_communis	pycom06g12300 pycom06g12310 pycom111g00240 pycom17g00260
rosa_chinensis	RchiOBHm_Chr2g0176471 RchiOBHm_Chr2g0176481 RchiOBHm_Chr7g0188771 RchiOBHm_Chr7g0188781
rosa_laevigata	RLG00000004661 RLG00000004662 RLG00000022416
rosa_multiflora	Rmu_sc0000303.1_g000057 Rmu_sc0003642.1_g000008 Rmu_sc0011904.1_g000012
rosa_roxburghii	Rroxscaffold_2G00076370 Rroxscaffold_3G00266040 Rroxscaffold_3G00266050
rosa_rugosa	Rorug02G0595000 Rorug06G0493700
rosa_samantha	Rh2AG673300 Rh2BG684600 Rh2CG647400 Rh2DG698200 Rh7AG098300 Rh7AG098400 Rh7BG099900 Rh7BG100200 Rh7CG101600 Rh7CG101700 Rh7DG100100 Rh7DG100200
rosa_wichuraiana	Rw0G010390 Rw2G055220 Rw7G008480 Rw7G008490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	63, 2516
AasI	GACNNNNNNGTC	1 cut(s)	581
AatII	GACGTC	1 cut(s)	579
Acc36I	ACCTGC	1 cut(s)	1804
AccB7I	CCANNNNNTGG	3 cut(s)	365, 1487, 2530
AccII	CGCG	5 cut(s)	144, 149, 636, 722, 2040
AciI	CCGC	4 cut(s)	147, 149, 419, 1373
AclI	AACGTT	1 cut(s)	946
AclWI	GGATC	8 cut(s)	196, 1874, 2187, 2200, 2342, 2474, 2487, 2768
AcoI	YGGCCR	3 cut(s)	235, 857, 1443
AcsI	RAATTY	6 cut(s)	245, 761, 995, 1928, 2055, 2811
AcuI	CTGAAG	2 cut(s)	675, 1524
AcyI	GRCGYC	2 cut(s)	576, 2374
AdeI	CACNNNGTG	1 cut(s)	1439
AfaI	GTAC	8 cut(s)	594, 685, 1479, 1559, 1652, 1789, 1901, 2333
AfiI	CCNNNNNNNGG	6 cut(s)	365, 418, 1414, 1487, 2105, 2530
AjnI	CCWGG	6 cut(s)	854, 1600, 1708, 2082, 2523, 2556
AjuI	GAANNNNNNNTTGG	2 cut(s)	2000, 2032
AleI	CACNNNNGTG	1 cut(s)	1895
Alw26I	GTCTC	4 cut(s)	1513, 1540, 2055, 2701
AlwI	GGATC	8 cut(s)	196, 1874, 2187, 2200, 2342, 2474, 2487, 2768
AlwNI	CAGNNNCTG	2 cut(s)	1475, 2530
AoxI	GGCC	7 cut(s)	235, 857, 932, 1022, 1443, 1598, 1940
ApeKI	GCWGC	6 cut(s)	220, 281, 980, 1096, 1375, 1517
ApoI	RAATTY	6 cut(s)	245, 761, 995, 1928, 2055, 2811
AseI	ATTAAT	1 cut(s)	2124
Asp700I	GAANNNNTTC	1 cut(s)	1733
AspLEI	GCGC	3 cut(s)	146, 2040, 2563
AspS9I	GGNCC	7 cut(s)	702, 1022, 1598, 1838, 1940, 2080, 2744
AsuHPI	GGTGA	3 cut(s)	626, 1751, 2261
AsuII	TTCGAA	2 cut(s)	72, 2815
AvaII	GGWCC	4 cut(s)	702, 1838, 2080, 2744
BalI	TGGCCA	3 cut(s)	237, 859, 1445
BamHI	GGATCC	2 cut(s)	2192, 2479
BbsI	GAAGAC	1 cut(s)	1765
BbvCI	CCTCAGC	1 cut(s)	423
BbvI	GCAGC	6 cut(s)	232, 268, 992, 1108, 1362, 1504
BccI	CCATC	9 cut(s)	748, 779, 823, 1628, 1712, 1739, 1952, 2531, 2834
BciT130I	CCWGG	6 cut(s)	856, 1602, 1710, 2084, 2525, 2558
BclI	TGATCA	1 cut(s)	307
BcoDI	GTCTC	4 cut(s)	1513, 1540, 2055, 2701
BfaI	CTAG	8 cut(s)	162, 224, 320, 891, 1092, 1893, 2786, 2836
BfmI	CTRYAG	3 cut(s)	1935, 2065, 2184
BfuAI	ACCTGC	1 cut(s)	1804
BglII	AGATCT	2 cut(s)	1088, 2180
BisI	GCNGC	8 cut(s)	147, 221, 282, 420, 981, 1097, 1376, 1518
BlsI	GCNGC	8 cut(s)	148, 222, 283, 421, 982, 1098, 1377, 1519
Bme1390I	CCNGG	6 cut(s)	856, 1602, 1710, 2084, 2525, 2558
Bme18I	GGWCC	4 cut(s)	702, 1838, 2080, 2744
BmgT120I	GGNCC	7 cut(s)	702, 1022, 1598, 1838, 1940, 2080, 2744
BmiI	GGNNCC	4 cut(s)	1839, 2081, 2194, 2481
BmrFI	CCNGG	6 cut(s)	856, 1602, 1710, 2084, 2525, 2558
BmrI	ACTGGG	1 cut(s)	2086
BmsI	GCATC	7 cut(s)	334, 820, 1036, 1063, 1394, 2214, 2599
BmuI	ACTGGG	1 cut(s)	2086
BpiI	GAAGAC	1 cut(s)	1765
BpmI	CTGGAG	2 cut(s)	1731, 2507
Bpu10I	CCTNAGC	1 cut(s)	423
Bpu14I	TTCGAA	2 cut(s)	72, 2815
BpuEI	CTTGAG	3 cut(s)	521, 1495, 2776
Bsa29I	ATCGAT	2 cut(s)	328, 378
BsaAI	YACGTR	1 cut(s)	1102
BsaBI	GATNNNNATC	2 cut(s)	606, 1749
BsaHI	GRCGYC	2 cut(s)	576, 2374
BsaI	GGTCTC	1 cut(s)	1513
BsaJI	CCNNGG	3 cut(s)	147, 705, 1708
BsaWI	WCCGGW	1 cut(s)	1343
BsaXI	ACNNNNNCTCC	2 cut(s)	1755, 1785
Bsc4I	CCNNNNNNNGG	6 cut(s)	365, 418, 1414, 1487, 2105, 2530
Bse1I	ACTGG	8 cut(s)	246, 914, 919, 1207, 1949, 2081, 2447, 2715
Bse3DI	GCAATG	1 cut(s)	521
Bse8I	GATNNNNATC	2 cut(s)	606, 1749
BseBI	CCWGG	6 cut(s)	856, 1602, 1710, 2084, 2525, 2558
BseCI	ATCGAT	2 cut(s)	328, 378
BseDI	CCNNGG	3 cut(s)	147, 705, 1708
BseGI	GGATG	3 cut(s)	267, 1750, 1855
BseJI	GATNNNNATC	2 cut(s)	606, 1749
BseLI	CCNNNNNNNGG	6 cut(s)	365, 418, 1414, 1487, 2105, 2530
BseMI	GCAATG	1 cut(s)	521
BseMII	CTCAG	6 cut(s)	414, 1140, 1773, 1911, 2330, 2727
BseNI	ACTGG	8 cut(s)	246, 914, 919, 1207, 1949, 2081, 2447, 2715
BseXI	GCAGC	6 cut(s)	232, 268, 992, 1108, 1362, 1504
BsgI	GTGCAG	1 cut(s)	422
Bsh1236I	CGCG	5 cut(s)	144, 149, 636, 722, 2040
BshFI	GGCC	7 cut(s)	237, 859, 934, 1024, 1445, 1600, 1942
BshVI	ATCGAT	2 cut(s)	328, 378
BsiSI	CCGG	1 cut(s)	1344
BslFI	GGGAC	4 cut(s)	569, 1851, 2093, 2775
BslI	CCNNNNNNNGG	6 cut(s)	365, 418, 1414, 1487, 2105, 2530
BsmAI	GTCTC	4 cut(s)	1513, 1540, 2055, 2701
BsmFI	GGGAC	4 cut(s)	569, 1851, 2093, 2775
BsmI	GAATGC	1 cut(s)	907
BsnI	GGCC	7 cut(s)	237, 859, 934, 1024, 1445, 1600, 1942
Bso31I	GGTCTC	1 cut(s)	1513
Bsp119I	TTCGAA	2 cut(s)	72, 2815
Bsp1407I	TGTACA	3 cut(s)	592, 1557, 1899
Bsp19I	CCATGG	1 cut(s)	705
BspACI	CCGC	4 cut(s)	147, 149, 419, 1373
BspANI	GGCC	7 cut(s)	237, 859, 934, 1024, 1445, 1600, 1942
BspCNI	CTCAG	6 cut(s)	415, 1141, 1772, 1912, 2331, 2728
BspDI	ATCGAT	2 cut(s)	328, 378
BspFNI	CGCG	5 cut(s)	144, 149, 636, 722, 2040
BspHI	TCATGA	1 cut(s)	1069
BspLI	GGNNCC	4 cut(s)	1839, 2081, 2194, 2481
BspMI	ACCTGC	1 cut(s)	1804
BspPI	GGATC	8 cut(s)	196, 1874, 2187, 2200, 2342, 2474, 2487, 2768
BspQI	GCTCTTC	1 cut(s)	2737
BspT104I	TTCGAA	2 cut(s)	72, 2815
BspTNI	GGTCTC	1 cut(s)	1513
BsrDI	GCAATG	1 cut(s)	521
BsrGI	TGTACA	3 cut(s)	592, 1557, 1899
BsrI	ACTGG	8 cut(s)	246, 914, 919, 1207, 1949, 2081, 2447, 2715
BssECI	CCNNGG	3 cut(s)	147, 705, 1708
BssNI	GRCGYC	2 cut(s)	576, 2374
BssT1I	CCWWGG	1 cut(s)	705
Bst2UI	CCWGG	6 cut(s)	856, 1602, 1710, 2084, 2525, 2558
Bst4CI	ACNGT	6 cut(s)	214, 1236, 1330, 1477, 1555, 2287
Bst6I	CTCTTC	3 cut(s)	2574, 2699, 2737
BstACI	GRCGYC	2 cut(s)	576, 2374
BstAPI	GCANNNNNTGC	1 cut(s)	1206
BstAUI	TGTACA	3 cut(s)	592, 1557, 1899
BstBAI	YACGTR	1 cut(s)	1102
BstBI	TTCGAA	2 cut(s)	72, 2815
BstC8I	GCNNGC	8 cut(s)	151, 269, 371, 405, 932, 936, 1461, 1917
BstDEI	CTNAG	8 cut(s)	423, 1149, 1759, 1920, 1983, 2339, 2367, 2736
BstDSI	CCRYGG	2 cut(s)	147, 705
BstENI	CCTNNNNNAGG	1 cut(s)	2103
BstF5I	GGATG	3 cut(s)	267, 1750, 1855
BstFNI	CGCG	5 cut(s)	144, 149, 636, 722, 2040
BstHHI	GCGC	3 cut(s)	146, 2040, 2563
BstMAI	GTCTC	4 cut(s)	1513, 1540, 2055, 2701
BstMWI	GCNNNNNNNGC	4 cut(s)	150, 839, 1206, 1469
BstNI	CCWGG	6 cut(s)	856, 1602, 1710, 2084, 2525, 2558
BstNSI	RCATGY	3 cut(s)	271, 842, 1919
BstSCI	CCNGG	6 cut(s)	854, 1600, 1708, 2082, 2523, 2556
BstSFI	CTRYAG	3 cut(s)	1935, 2065, 2184
BstUI	CGCG	5 cut(s)	144, 149, 636, 722, 2040
BstV1I	GCAGC	6 cut(s)	232, 268, 992, 1108, 1362, 1504
BstV2I	GAAGAC	1 cut(s)	1765
BstX2I	RGATCY	4 cut(s)	1088, 2180, 2192, 2479
BstXI	CCANNNNNNTGG	1 cut(s)	2203
BstYI	RGATCY	4 cut(s)	1088, 2180, 2192, 2479
Bsu15I	ATCGAT	2 cut(s)	328, 378
BsuRI	GGCC	7 cut(s)	237, 859, 934, 1024, 1445, 1600, 1942
BsuTUI	ATCGAT	2 cut(s)	328, 378
BtgI	CCRYGG	2 cut(s)	147, 705
BtsCI	GGATG	3 cut(s)	267, 1750, 1855
BtsIMutI	CAGTG	6 cut(s)	567, 1200, 1241, 1321, 1326, 1560
BveI	ACCTGC	1 cut(s)	1804
Cac8I	GCNNGC	8 cut(s)	151, 269, 371, 405, 932, 936, 1461, 1917
CaiI	CAGNNNCTG	2 cut(s)	1475, 2530
CciI	TCATGA	1 cut(s)	1069
CfoI	GCGC	3 cut(s)	146, 2040, 2563
Cfr13I	GGNCC	7 cut(s)	702, 1022, 1598, 1838, 1940, 2080, 2744
Cfr42I	CCGCGG	1 cut(s)	150
ClaI	ATCGAT	2 cut(s)	328, 378
CseI	GACGC	1 cut(s)	2363
Csp6I	GTAC	8 cut(s)	593, 684, 1478, 1558, 1651, 1788, 1900, 2332
CviQI	GTAC	8 cut(s)	593, 684, 1478, 1558, 1651, 1788, 1900, 2332
DdeI	CTNAG	8 cut(s)	423, 1149, 1759, 1920, 1983, 2339, 2367, 2736
DraI	TTTAAA	1 cut(s)	114
DraIII	CACNNNGTG	1 cut(s)	1439
DrdI	GACNNNNNNGTC	1 cut(s)	581
DseDI	GACNNNNNNGTC	1 cut(s)	581
EaeI	YGGCCR	3 cut(s)	235, 857, 1443
Eam1104I	CTCTTC	3 cut(s)	2574, 2699, 2737
EarI	CTCTTC	3 cut(s)	2574, 2699, 2737
Eco130I	CCWWGG	1 cut(s)	705
Eco31I	GGTCTC	1 cut(s)	1513
Eco47I	GGWCC	4 cut(s)	702, 1838, 2080, 2744
Eco57I	CTGAAG	2 cut(s)	675, 1524
EcoNI	CCTNNNNNAGG	1 cut(s)	2103
EcoO109I	RGGNCCY	3 cut(s)	1022, 1940, 2080
EcoRI	GAATTC	2 cut(s)	245, 2055
EcoRII	CCWGG	6 cut(s)	854, 1600, 1708, 2082, 2523, 2556
EcoT14I	CCWWGG	1 cut(s)	705
EcoT22I	ATGCAT	2 cut(s)	907, 1387
ErhI	CCWWGG	1 cut(s)	705
FalI	AAGNNNNNCTT	2 cut(s)	1266, 1298
FaqI	GGGAC	4 cut(s)	569, 1851, 2093, 2775
FauI	CCCGC	1 cut(s)	142
FauNDI	CATATG	2 cut(s)	886, 1768
FbaI	TGATCA	1 cut(s)	307
Fnu4HI	GCNGC	8 cut(s)	147, 221, 282, 420, 981, 1097, 1376, 1518
FokI	GGATG	3 cut(s)	274, 1757, 1862
Fsp4HI	GCNGC	8 cut(s)	147, 221, 282, 420, 981, 1097, 1376, 1518
FspBI	CTAG	8 cut(s)	162, 224, 320, 891, 1092, 1893, 2786, 2836
GlaI	GCGC	3 cut(s)	145, 2039, 2562
GluI	GCNGC	8 cut(s)	147, 221, 282, 420, 981, 1097, 1376, 1518
GsuI	CTGGAG	2 cut(s)	1731, 2507
HaeIII	GGCC	7 cut(s)	237, 859, 934, 1024, 1445, 1600, 1942
HapII	CCGG	1 cut(s)	1344
HgaI	GACGC	1 cut(s)	2363
HhaI	GCGC	3 cut(s)	146, 2040, 2563
Hin1I	GRCGYC	2 cut(s)	576, 2374
Hin6I	GCGC	3 cut(s)	144, 2038, 2561
HinP1I	GCGC	3 cut(s)	144, 2038, 2561
HpaII	CCGG	1 cut(s)	1344
HphI	GGTGA	3 cut(s)	626, 1751, 2261
Hpy166II	GTNNAC	7 cut(s)	702, 1241, 1558, 1653, 1900, 2092, 2206
Hpy188I	TCNGA	9 cut(s)	312, 655, 753, 1248, 1273, 1543, 1807, 2121, 2737
Hpy8I	GTNNAC	7 cut(s)	702, 1241, 1558, 1653, 1900, 2092, 2206
Hpy99I	CGWCG	1 cut(s)	2379
HpyAV	CCTTC	8 cut(s)	555, 1147, 1723, 1802, 2428, 2493, 2682, 2735
HpyCH4III	ACNGT	6 cut(s)	214, 1236, 1330, 1477, 1555, 2287
HpyCH4IV	ACGT	4 cut(s)	576, 624, 946, 1101
HpyF10VI	GCNNNNNNNGC	4 cut(s)	150, 839, 1206, 1469
HpyF3I	CTNAG	8 cut(s)	423, 1149, 1759, 1920, 1983, 2339, 2367, 2736
HpySE526I	ACGT	4 cut(s)	576, 624, 946, 1101
Hsp92I	GRCGYC	2 cut(s)	576, 2374
HspAI	GCGC	3 cut(s)	144, 2038, 2561
Ksp22I	TGATCA	1 cut(s)	307
KspI	CCGCGG	1 cut(s)	150
LguI	GCTCTTC	1 cut(s)	2737
LmnI	GCTCC	3 cut(s)	1763, 2527, 2806
Lsp1109I	GCAGC	6 cut(s)	232, 268, 992, 1108, 1362, 1504
LweI	GCATC	7 cut(s)	334, 820, 1036, 1063, 1394, 2214, 2599
MaeI	CTAG	8 cut(s)	162, 224, 320, 891, 1092, 1893, 2786, 2836
MaeII	ACGT	4 cut(s)	576, 624, 946, 1101
MaeIII	GTNAC	8 cut(s)	352, 614, 1136, 1219, 1324, 1888, 1978, 2458
MboII	GAAGA	7 cut(s)	387, 1765, 2546, 2591, 2653, 2716, 2724
MfeI	CAATTG	3 cut(s)	989, 1056, 2213
MflI	RGATCY	4 cut(s)	1088, 2180, 2192, 2479
MlsI	TGGCCA	3 cut(s)	237, 859, 1445
MluNI	TGGCCA	3 cut(s)	237, 859, 1445
MlyI	GAGTC	4 cut(s)	310, 349, 860, 1331
MmeI	TCCRAC	2 cut(s)	1827, 2801
Mox20I	TGGCCA	3 cut(s)	237, 859, 1445
Mph1103I	ATGCAT	2 cut(s)	907, 1387
MroXI	GAANNNNTTC	1 cut(s)	1733
MscI	TGGCCA	3 cut(s)	237, 859, 1445
MslI	CAYNNNNRTG	4 cut(s)	299, 1071, 1502, 1895
Msp20I	TGGCCA	3 cut(s)	237, 859, 1445
MspA1I	CMGCKG	2 cut(s)	149, 1375
MspI	CCGG	1 cut(s)	1344
MspR9I	CCNGG	6 cut(s)	856, 1602, 1710, 2084, 2525, 2558
MunI	CAATTG	3 cut(s)	989, 1056, 2213
Mva1269I	GAATGC	1 cut(s)	907
MvaI	CCWGG	6 cut(s)	856, 1602, 1710, 2084, 2525, 2558
MvnI	CGCG	5 cut(s)	144, 149, 636, 722, 2040
MwoI	GCNNNNNNNGC	4 cut(s)	150, 839, 1206, 1469
NcoI	CCATGG	1 cut(s)	705
NdeI	CATATG	2 cut(s)	886, 1768
NlaIV	GGNNCC	4 cut(s)	1839, 2081, 2194, 2481
NmuCI	GTSAC	5 cut(s)	352, 614, 1324, 1888, 2458
NsiI	ATGCAT	2 cut(s)	907, 1387
NspI	RCATGY	3 cut(s)	271, 842, 1919
NspV	TTCGAA	2 cut(s)	72, 2815
OliI	CACNNNNGTG	1 cut(s)	1895
PaeI	GCATGC	2 cut(s)	271, 1919
PagI	TCATGA	1 cut(s)	1069
PciSI	GCTCTTC	1 cut(s)	2737
PctI	GAATGC	1 cut(s)	907
PdmI	GAANNNNTTC	1 cut(s)	1733
PflFI	GACNNNGTC	1 cut(s)	212
PflMI	CCANNNNNTGG	3 cut(s)	365, 1487, 2530
PfoI	TCCNGGA	1 cut(s)	2523
PkrI	GCNGC	8 cut(s)	148, 222, 283, 421, 982, 1098, 1377, 1519
PleI	GAGTC	4 cut(s)	310, 349, 859, 1331
PpsI	GAGTC	4 cut(s)	310, 349, 859, 1331
Ppu21I	YACGTR	1 cut(s)	1102
PpuMI	RGGWCCY	1 cut(s)	2080
PshBI	ATTAAT	1 cut(s)	2124
PsiI	TTATAA	2 cut(s)	63, 2516
Psp1406I	AACGTT	1 cut(s)	946
Psp5II	RGGWCCY	1 cut(s)	2080
Psp6I	CCWGG	6 cut(s)	854, 1600, 1708, 2082, 2523, 2556
PspGI	CCWGG	6 cut(s)	854, 1600, 1708, 2082, 2523, 2556
PspN4I	GGNNCC	4 cut(s)	1839, 2081, 2194, 2481
PspPI	GGNCC	7 cut(s)	702, 1022, 1598, 1838, 1940, 2080, 2744
PspPPI	RGGWCCY	1 cut(s)	2080
PstNI	CAGNNNCTG	2 cut(s)	1475, 2530
PsuI	RGATCY	4 cut(s)	1088, 2180, 2192, 2479
PsyI	GACNNNGTC	1 cut(s)	212
RsaI	GTAC	8 cut(s)	594, 685, 1479, 1559, 1652, 1789, 1901, 2333
RsaNI	GTAC	8 cut(s)	593, 684, 1478, 1558, 1651, 1788, 1900, 2332
RseI	CAYNNNNRTG	4 cut(s)	299, 1071, 1502, 1895
SacII	CCGCGG	1 cut(s)	150
SapI	GCTCTTC	1 cut(s)	2737
SatI	GCNGC	8 cut(s)	147, 221, 282, 420, 981, 1097, 1376, 1518
Sau96I	GGNCC	7 cut(s)	702, 1022, 1598, 1838, 1940, 2080, 2744
SchI	GAGTC	4 cut(s)	310, 349, 860, 1331
ScrFI	CCNGG	6 cut(s)	856, 1602, 1710, 2084, 2525, 2558
SfaNI	GCATC	7 cut(s)	334, 820, 1036, 1063, 1394, 2214, 2599
SfcI	CTRYAG	3 cut(s)	1935, 2065, 2184
Sfr303I	CCGCGG	1 cut(s)	150
SfuI	TTCGAA	2 cut(s)	72, 2815
SgrBI	CCGCGG	1 cut(s)	150
SinI	GGWCC	4 cut(s)	702, 1838, 2080, 2744
SmiMI	CAYNNNNRTG	4 cut(s)	299, 1071, 1502, 1895
SmlI	CTYRAG	3 cut(s)	500, 1510, 2755
SmoI	CTYRAG	3 cut(s)	500, 1510, 2755
SphI	GCATGC	2 cut(s)	271, 1919
SsiI	CCGC	4 cut(s)	147, 149, 419, 1373
SspI	AATATT	1 cut(s)	1252
SspMI	CTAG	8 cut(s)	162, 224, 320, 891, 1092, 1893, 2786, 2836
StyD4I	CCNGG	6 cut(s)	854, 1600, 1708, 2082, 2523, 2556
StyI	CCWWGG	1 cut(s)	705
TaaI	ACNGT	6 cut(s)	214, 1236, 1330, 1477, 1555, 2287
TaiI	ACGT	4 cut(s)	579, 627, 949, 1104
TaqI	TCGA	8 cut(s)	72, 328, 378, 459, 2059, 2377, 2815, 2829
TaqII	GACCGA	2 cut(s)	1660, 2761
TatI	WGTACW	4 cut(s)	592, 1557, 1650, 1899
TauI	GCSGC	2 cut(s)	149, 422
TscAI	CASTG	6 cut(s)	574, 1207, 1241, 1321, 1333, 1560
TseFI	GTSAC	5 cut(s)	352, 614, 1324, 1888, 2458
TseI	GCWGC	6 cut(s)	220, 281, 980, 1096, 1375, 1517
Tsp45I	GTSAC	5 cut(s)	352, 614, 1324, 1888, 2458
TspDTI	ATGAA	9 cut(s)	59, 238, 620, 729, 758, 1596, 1866, 1875, 2295
TspGWI	ACGGA	2 cut(s)	1766, 2223
TspRI	CASTG	6 cut(s)	574, 1207, 1241, 1321, 1333, 1560
Tth111I	GACNNNGTC	1 cut(s)	212
Van91I	CCANNNNNTGG	3 cut(s)	365, 1487, 2530
VpaK11BI	GGWCC	4 cut(s)	702, 1838, 2080, 2744
VspI	ATTAAT	1 cut(s)	2124
XagI	CCTNNNNNAGG	1 cut(s)	2103
XapI	RAATTY	6 cut(s)	245, 761, 995, 1928, 2055, 2811
XceI	RCATGY	3 cut(s)	271, 842, 1919
XcmI	CCANNNNNNNNNTGG	1 cut(s)	366
XmnI	GAANNNNTTC	1 cut(s)	1733
XspI	CTAG	8 cut(s)	162, 224, 320, 891, 1092, 1893, 2786, 2836
ZraI	GACGTC	1 cut(s)	577
Zsp2I	ATGCAT	2 cut(s)	907, 1387

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.