Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0001301

Physical Location & Seq

Genomic Coordinates Reverse (-)

83199530 .. 83207448

Gene Structure 7919 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000022416

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2691 bp

ATGGCGACCACGACTTCTCGTTTACCCTTCTTCTTCTGTGTGCTTATCTCGGTCGGAATTCTAGTCGCCGAGTCCAAATTTATGGTCTATAACACCTCCCAAGGGATAGTCCCCGGTAAAGTTAACGTCCACTTGGTCCCTCACACCCACGACGACGTCGGATGGTTGAAGACCGTTGATCAGTACTATGTTGGCTCCAACAATTCCATCCAGGGGGCTTGTGTGCAAAATGTGTTGGACTCTTTGATTCCTGCTCTGTTGGCTGACAAGAATCGGAAATTCATCTACGTTGAAATTGCTTTTTTCCAGCGCTGGTGGAGAGATCAGAGTGATGCCGTCCAGCGTATAGTCACTGACCTTGTCAGCTCGGGTCAACTAGAATTCATAAATGGGGGCATGTGTATGCATGATGAAGCAGCTACGCATTATGTTGACATGATTGATCAGACCACTCTTGGGCATCGCTTTATTAAAAGGGAATTCGATGTGACTCCAAGAATTGGATGGCAAATTGATCCTTTTGGACATTCGGCTGTCCAGGCCTACTTGTTGGGAGCACAAGTTGGATTCGACTCACTTTACTTTGGTCGAATAGATTACCAGGACAGGGAAAAACGCAAAAATGACAAGAGTCTTGAGTTTGTTTGGCAGGGTTCTAAGAGTCTCGGTTCATCTGCTGAGATTTTTTCTGGTGCTTTCCCTGAGAATTATGAACCTCCTTCTGATTTCTATTTTGAAGTTAATGATAAATCCCCAATAGTCCAGGATGACATCACTTTGTTTGATTACAATGTTCAAGAACGAGTTAATGACTTTGTTGCTGCTGCAGTATCACAGGCTAACATAACTCGAACAAATCATATTATGTGGACCATGGGAACAGACTTCAAGTATCAGTATGCACACACCTGGTTCCGTCAAATGGACAAGCTCATTCATTATGTCAACAAGTCTGAGCAACTAGTTGCAACTTCACTTGCTCACTTGGTTGAGTCTGCATCATATACTGGATCTGGAAACCCAACTACAAAGTTTCAACAGTGTCTACTTCTGAATATAAGTTATTGCCCTGCATCAGAAGTAACTTTGTCTCAGGGGAAAAAATTGATTGTCGTGGTGTACAATTCTCTTGGATGGAAGAGGGATGACACAGTCCGAATTCCTGTTATCAATGAAGATGTCATTGTTCATGATTCTGAGGGAAAAGCAATCGATTCACAGCTTCTTCCTCTAGATGATGTCCATCTGGGCTTAAGGAACTACTATGTTAAGGCATACTTGGGTCAAACTCCAACAAAGACACCAAAGTATTGGCTTGTGTTTACAGTATCCGTACCACCACTTGGTTTTAGCACATACACTATCTCGGGTGCCGAAGGGGCAGGTGCTAGCTCAACCAGATCATCTGTATACACCTTTCAAAGCAGAGAAAAGTTGACTATTGAAGTTGGTCAAGGAAATGTGAAACTCTCTTTTTCCACAGATCAAGGCAAAATGACTAATTATGTCAATAGCAGAAGCTTGGTGGAAGAATCGGTTCAGCAGTCTTACAGCTTTTATAATGGGTATAATGGCAGTAATGATAAGGCTCCTCTGATTCCTCAGTTTGGCACAGGGATGAGTGTCACCATTGCTTCTTTGACTGTTATACGTGGACCAGTAATAGATGAAGTACATCAACAGATTAATTCGTGGATACATCAGATCACTAGACTTCACAAGGAAAAGGAGCATGTTGAAGTTGAGTTTATTGTTGGCCCTATACCTACTGATGATGGAATTGGAAAAGAAGTTGTAACTCAATTAGACACTGCCATGGCTACCAACAAAACGTTCTACACTGATTCTAATGGGCGTGATTTTATCAAAAGGATTCGCGACTATAGAACAGATTGGGACTTCAAAGTGAACCAACCGGTTGCTGGAAATTATTACCCAATTAATCTTGGGATATACATGCAAGATAATAGGATAGAATTTTCAGTTTTGGTCGATCGGTCACTAGGGGGATCCAGCACAGTGGATGGTCAGATAGAGCTGATGCTTCACAGGAGACTACTACTTGATGACTCAAGAGGTGTTGCCGAGGCTCTAAATGAAACTGTCTGTGTTTCCGATGAATGCACAGGACTAAGAGTCCTAGGGAAATTTTACTTCAGAATTGACCCTTTGGGAGATGGTGCTAAGTGGCGTCGTTCTTTTGGTCAGGAGATATATTCACCTCTTCTCCTAGCCTTTGCTGAACAGGATGGAGATAACTGGAAGAATTCTCATGCAACCACTTTTTCTGGGATTGATTCCTCATATAGTTTACCTGATAATGTTGCTCTTATAACTCTTCAGGAGCTTGATGATGGAAAAATTCTCATTCGCTTAGCACATTTATACGAGGTTGGGGAGGACAAGGATTTTTCTGTAATGGCAAATGTAGAGCTGAAGAAGCTCTTCCCCAGGAAGAAGATTGGCAAAGCGACAGAAATGAACTTATCAGCTAATCAAGAAAGGACAGAAATGGAGAGAAAGAGACTGGTATGGAAAAAGGAAGGGGGCTCTTCAGGAACGGAAAAGGGTGTGAGGGGTGGACCTGTTGATCCTGCAAAACTAGTTGTGGAACTTGCCCCAATGGAAATCCGCACCTTTCTCATTGACTTGGAGCAGGAGTTCCACCGCCGCCATCATGATGATGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0001775 GO:0002252 GO:0002263 GO:0002274 GO:0002275 GO:0002283 GO:0002366 GO:0002376 GO:0002443 GO:0002444 GO:0002446 GO:0003674 GO:0003824 GO:0004553 GO:0004559 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005766 GO:0005773 GO:0005775 GO:0005975 GO:0005996 GO:0006013 GO:0006464 GO:0006517 GO:0006807 GO:0006810 GO:0006887 GO:0006955 GO:0008150 GO:0008152 GO:0009056 GO:0009100 GO:0009311 GO:0009313 GO:0009987 GO:0012505 GO:0015923 GO:0016052 GO:0016192 GO:0016787 GO:0016798 GO:0019318 GO:0019538 GO:0030141 GO:0031410 GO:0031974 GO:0031982 GO:0031983 GO:0032940 GO:0034774 GO:0035578 GO:0036211 GO:0036230 GO:0042119 GO:0042582 GO:0043170 GO:0043202 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043299 GO:0043312 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044281 GO:0044421 GO:0044422 GO:0044424 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045055 GO:0045321 GO:0046903 GO:0050896 GO:0051179 GO:0051234 GO:0060205 GO:0070013 GO:0071704 GO:0097708 GO:0099503 GO:1901135 GO:1901564 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Alpha-mann_mid Glyco_hydro_38 Glyco_hydro_38C

Protein Analysis

897

Amino Acids

101.04

Weight (kDa)

5.61

Isoelectric Point (pI)

37.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_38N	PF01074	42 - 322	1.4e-77	Glycosyl hydrolases family 38 N-terminal domain
Laman-like_dom	PF21260	404 - 455	3.2e-12	Lysosomal alpha-mannosidase-like, central domain
Glyco_hydro_38C	PF07748	484 - 692	5e-41	Glycosyl hydrolases family 38 C-terminal domain
Glyco_hydro38C2	PF17677	774 - 880	8.3e-09	Glycosyl hydrolases family 38 C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0001301)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13980 AT5G13980 AT5G13980
fragaria_vesca	FvH4_5g09420 FvH4_5g09421 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272 FvH4_6g54272
malus_domestica	MD06G1131600.v1.1 MD09G1002900.v1.1 MD17G1007000.v1.1
prunus_persica	Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.3G313300_v2.0.a1 Prupe.5G142600_v2.0.a1 Prupe.5G142600_v2.0.a1
pyrus_communis	pycom06g12300 pycom06g12310 pycom111g00240 pycom17g00260
rosa_chinensis	RchiOBHm_Chr2g0176471 RchiOBHm_Chr2g0176481 RchiOBHm_Chr7g0188771 RchiOBHm_Chr7g0188781
rosa_laevigata	RLG00000004661 RLG00000004662 RLG00000022416
rosa_multiflora	Rmu_sc0000303.1_g000057 Rmu_sc0003642.1_g000008 Rmu_sc0011904.1_g000012
rosa_roxburghii	Rroxscaffold_2G00076370 Rroxscaffold_3G00266040 Rroxscaffold_3G00266050
rosa_rugosa	Rorug02G0595000 Rorug06G0493700
rosa_samantha	Rh2AG673300 Rh2BG684600 Rh2CG647400 Rh2DG698200 Rh7AG098300 Rh7AG098400 Rh7BG099900 Rh7BG100200 Rh7CG101600 Rh7CG101700 Rh7DG100100 Rh7DG100200
rosa_wichuraiana	Rw0G010390 Rw2G055220 Rw7G008480 Rw7G008490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1560, 2333
AarI	CACCTGC	1 cut(s)	1373
AatII	GACGTC	1 cut(s)	159
Acc36I	ACCTGC	1 cut(s)	1373
AccB1I	GGYRCC	1 cut(s)	1370
AccB7I	CCANNNNNTGG	2 cut(s)	500, 1343
AccI	GTMKAC	2 cut(s)	1045, 1410
AccII	CGCG	1 cut(s)	1878
AciI	CCGC	3 cut(s)	2632, 2668, 2671
AclI	AACGTT	1 cut(s)	1832
AclWI	GGATC	5 cut(s)	509, 1018, 2004, 2017, 2585
AcuI	CTGAAG	4 cut(s)	2140, 2324, 2456, 2538
AcyI	GRCGYC	2 cut(s)	156, 2191
AfaI	GTAC	4 cut(s)	185, 1121, 1335, 1674
AfeI	AGCGCT	1 cut(s)	311
AfiI	CCNNNNNNNGG	9 cut(s)	102, 213, 456, 500, 607, 922, 1343, 1607, 1922
AflII	CTTAAG	1 cut(s)	1252
AgeI	ACCGGT	1 cut(s)	1915
AhdI	GACNNNNNGTC	1 cut(s)	2135
AhlI	ACTAGT	2 cut(s)	961, 2602
AjnI	CCWGG	6 cut(s)	210, 537, 600, 762, 908, 2450
AjuI	GAANNNNNNNTTGG	2 cut(s)	1817, 1849
AloI	GAACNNNNNNTCC	2 cut(s)	2645, 2677
Alw21I	GWGCWC	1 cut(s)	559
Alw26I	GTCTC	4 cut(s)	668, 1095, 2047, 2518
AlwI	GGATC	5 cut(s)	509, 1018, 2004, 2017, 2585
Ama87I	CYCGRG	2 cut(s)	367, 1366
Aor51HI	AGCGCT	1 cut(s)	311
AoxI	GGCC	2 cut(s)	540, 1756
ApeKI	GCWGC	3 cut(s)	416, 821, 824
ArsI	GACNNNNNNTTYG	2 cut(s)	69, 101
AseI	ATTAAT	2 cut(s)	1686, 1941
AsiGI	ACCGGT	1 cut(s)	1915
Asp700I	GAANNNNTTC	2 cut(s)	1536, 2444
AspA2I	CCTAGG	1 cut(s)	2140
AspLEI	GCGC	1 cut(s)	312
AspS9I	GGNCC	5 cut(s)	136, 870, 1655, 1757, 2582
AsuC2I	CCSGG	1 cut(s)	114
AsuHPI	GGTGA	2 cut(s)	1618, 2211
AsuNHI	GCTAGC	1 cut(s)	1388
AvaI	CYCGRG	2 cut(s)	367, 1366
AvaII	GGWCC	4 cut(s)	136, 870, 1655, 2582
AvrII	CCTAGG	1 cut(s)	2140
BamHI	GGATCC	1 cut(s)	2009
BanI	GGYRCC	1 cut(s)	1370
BanII	GRGCYC	1 cut(s)	2552
BbsI	GAAGAC	1 cut(s)	176
Bbv12I	GWGCWC	1 cut(s)	559
BbvI	GCAGC	3 cut(s)	428, 808, 811
BceAI	ACGGC	1 cut(s)	320
BciT130I	CCWGG	6 cut(s)	212, 539, 602, 764, 910, 2452
BciVI	GTATCC	2 cut(s)	1339, 1689
BclI	TGATCA	2 cut(s)	178, 442
BcnI	CCSGG	1 cut(s)	114
BcoDI	GTCTC	4 cut(s)	668, 1095, 2047, 2518
BcuI	ACTAGT	2 cut(s)	961, 2602
BfmI	CTRYAG	2 cut(s)	825, 1882
BfoI	RGCGCY	1 cut(s)	313
BfrI	CTTAAG	1 cut(s)	1252
BfuAI	ACCTGC	1 cut(s)	1373
BfuI	GTATCC	2 cut(s)	1339, 1689
BglI	GCCNNNNNGGC	1 cut(s)	1379
BisI	GCNGC	4 cut(s)	417, 822, 825, 2671
BlnI	CCTAGG	1 cut(s)	2140
BlpI	GCTNAGC	1 cut(s)	2374
BlsI	GCNGC	4 cut(s)	418, 823, 826, 2672
BmcAI	AGTACT	1 cut(s)	185
Bme1390I	CCNGG	7 cut(s)	114, 212, 539, 602, 764, 910, 2452
Bme18I	GGWCC	4 cut(s)	136, 870, 1655, 2582
BmeRI	GACNNNNNGTC	1 cut(s)	2135
BmeT110I	CYCGRG	2 cut(s)	367, 1366
BmgT120I	GGNCC	5 cut(s)	136, 870, 1655, 1757, 2582
BmiI	GGNNCC	6 cut(s)	138, 196, 914, 1372, 1590, 2011
BmrFI	CCNGG	7 cut(s)	114, 212, 539, 602, 764, 910, 2452
BmsI	GCATC	6 cut(s)	322, 469, 1007, 1082, 2031, 2674
BmtI	GCTAGC	1 cut(s)	1392
BoxI	GACNNNNGTC	1 cut(s)	630
BpiI	GAAGAC	1 cut(s)	176
Bpu1102I	GCTNAGC	1 cut(s)	2374
BpuEI	CTTGAG	2 cut(s)	656, 2056
BpuMI	CCSGG	1 cut(s)	114
Bsa29I	ATCGAT	1 cut(s)	1212
BsaAI	YACGTR	1 cut(s)	1652
BsaBI	GATNNNNATC	1 cut(s)	1242
BsaHI	GRCGYC	2 cut(s)	156, 2191
BsaJI	CCNNGG	8 cut(s)	100, 112, 211, 873, 1815, 2085, 2140, 2450
BsaWI	WCCGGW	1 cut(s)	1915
BsaXI	ACNNNNNCTCC	6 cut(s)	546, 576, 2211, 2241, 2645, 2675
Bsc4I	CCNNNNNNNGG	9 cut(s)	102, 213, 456, 500, 607, 922, 1343, 1607, 1922
Bse118I	RCCGGY	1 cut(s)	1915
Bse1I	ACTGG	4 cut(s)	1012, 1658, 2264, 2532
Bse3DI	GCAATG	1 cut(s)	1629
Bse8I	GATNNNNATC	1 cut(s)	1242
BseBI	CCWGG	6 cut(s)	212, 539, 602, 764, 910, 2452
BseCI	ATCGAT	1 cut(s)	1212
BseDI	CCNNGG	8 cut(s)	100, 112, 211, 873, 1815, 2085, 2140, 2450
BseGI	GGATG	9 cut(s)	167, 207, 509, 772, 1139, 1150, 1623, 2029, 2254
BseJI	GATNNNNATC	1 cut(s)	1242
BseLI	CCNNNNNNNGG	9 cut(s)	102, 213, 456, 500, 607, 922, 1343, 1607, 1922
BseMI	GCAATG	1 cut(s)	1629
BseMII	CTCAG	6 cut(s)	669, 693, 945, 1106, 1188, 1616
BseNI	ACTGG	4 cut(s)	1012, 1658, 2264, 2532
BseRI	GAGGAG	1 cut(s)	1581
BseXI	GCAGC	3 cut(s)	428, 808, 811
Bsh1236I	CGCG	1 cut(s)	1878
Bsh1285I	CGRYCG	2 cut(s)	54, 1996
BshFI	GGCC	2 cut(s)	542, 1758
BshNI	GGYRCC	1 cut(s)	1370
BshTI	ACCGGT	1 cut(s)	1915
BshVI	ATCGAT	1 cut(s)	1212
BsiEI	CGRYCG	2 cut(s)	54, 1996
BsiHKAI	GWGCWC	1 cut(s)	559
BsiHKCI	CYCGRG	2 cut(s)	367, 1366
BsiSI	CCGG	2 cut(s)	114, 1916
BslFI	GGGAC	3 cut(s)	95, 122, 1910
BslI	CCNNNNNNNGG	9 cut(s)	102, 213, 456, 500, 607, 922, 1343, 1607, 1922
BsmAI	GTCTC	4 cut(s)	668, 1095, 2047, 2518
BsmFI	GGGAC	3 cut(s)	95, 122, 1910
BsmI	GAATGC	1 cut(s)	2126
BsnI	GGCC	2 cut(s)	542, 1758
BsoBI	CYCGRG	2 cut(s)	367, 1366
Bsp1286I	GDGCHC	2 cut(s)	559, 2552
Bsp1407I	TGTACA	1 cut(s)	1119
Bsp1720I	GCTNAGC	1 cut(s)	2374
Bsp19I	CCATGG	2 cut(s)	873, 1815
Bsp68I	TCGCGA	1 cut(s)	1878
BspACI	CCGC	3 cut(s)	2632, 2668, 2671
BspANI	GGCC	2 cut(s)	542, 1758
BspCNI	CTCAG	6 cut(s)	670, 694, 946, 1105, 1189, 1615
BspDI	ATCGAT	1 cut(s)	1212
BspFNI	CGCG	1 cut(s)	1878
BspHI	TCATGA	2 cut(s)	1189, 2677
BspLI	GGNNCC	6 cut(s)	138, 196, 914, 1372, 1590, 2011
BspMAI	CTGCAG	1 cut(s)	829
BspMI	ACCTGC	1 cut(s)	1373
BspOI	GCTAGC	1 cut(s)	1392
BspPI	GGATC	5 cut(s)	509, 1018, 2004, 2017, 2585
BspQI	GCTCTTC	2 cut(s)	2450, 2557
BspT107I	GGYRCC	1 cut(s)	1370
BspTI	CTTAAG	1 cut(s)	1252
BsrDI	GCAATG	1 cut(s)	1629
BsrFI	RCCGGY	1 cut(s)	1915
BsrGI	TGTACA	1 cut(s)	1119
BsrI	ACTGG	4 cut(s)	1012, 1658, 2264, 2532
BssAI	RCCGGY	1 cut(s)	1915
BssECI	CCNNGG	8 cut(s)	100, 112, 211, 873, 1815, 2085, 2140, 2450
BssNAI	GTATAC	1 cut(s)	1411
BssNI	GRCGYC	2 cut(s)	156, 2191
BssT1I	CCWWGG	4 cut(s)	100, 873, 1815, 2140
Bst1107I	GTATAC	1 cut(s)	1411
Bst2UI	CCWGG	6 cut(s)	212, 539, 602, 764, 910, 2452
Bst4CI	ACNGT	7 cut(s)	175, 1041, 1153, 1327, 1645, 2020, 2104
Bst6I	CTCTTC	5 cut(s)	1133, 2229, 2343, 2450, 2557
BstACI	GRCGYC	2 cut(s)	156, 2191
BstAFI	CTTAAG	1 cut(s)	1252
BstAUI	TGTACA	1 cut(s)	1119
BstBAI	YACGTR	1 cut(s)	1652
BstC8I	GCNNGC	1 cut(s)	1390
BstDSI	CCRYGG	2 cut(s)	873, 1815
BstF5I	GGATG	9 cut(s)	167, 207, 509, 772, 1139, 1150, 1623, 2029, 2254
BstFNI	CGCG	1 cut(s)	1878
BstH2I	RGCGCY	1 cut(s)	313
BstHHI	GCGC	1 cut(s)	312
BstMAI	GTCTC	4 cut(s)	668, 1095, 2047, 2518
BstMCI	CGRYCG	2 cut(s)	54, 1996
BstMWI	GCNNNNNNNGC	4 cut(s)	260, 539, 1379, 2440
BstNI	CCWGG	6 cut(s)	212, 539, 602, 764, 910, 2452
BstNSI	RCATGY	3 cut(s)	400, 1736, 1960
BstPAI	GACNNNNGTC	1 cut(s)	630
BstSCI	CCNGG	7 cut(s)	112, 210, 537, 600, 762, 908, 2450
BstSFI	CTRYAG	2 cut(s)	825, 1882
BstUI	CGCG	1 cut(s)	1878
BstV1I	GCAGC	3 cut(s)	428, 808, 811
BstV2I	GAAGAC	1 cut(s)	176
BstX2I	RGATCY	2 cut(s)	1010, 2009
BstXI	CCANNNNNNTGG	3 cut(s)	82, 1311, 2020
BstYI	RGATCY	2 cut(s)	1010, 2009
BstZ17I	GTATAC	1 cut(s)	1411
Bsu15I	ATCGAT	1 cut(s)	1212
BsuI	GTATCC	2 cut(s)	1339, 1689
BsuRI	GGCC	2 cut(s)	542, 1758
BsuTUI	ATCGAT	1 cut(s)	1212
BtgI	CCRYGG	2 cut(s)	873, 1815
BtgZI	GCGATG	1 cut(s)	446
BtsCI	GGATG	9 cut(s)	167, 207, 509, 772, 1139, 1150, 1623, 2029, 2254
BtsI	GCAGTG	1 cut(s)	1809
BtsIMutI	CAGTG	5 cut(s)	351, 1046, 1809, 1839, 2025
BtuMI	TCGCGA	1 cut(s)	1878
BveI	ACCTGC	1 cut(s)	1373
Cac8I	GCNNGC	1 cut(s)	1390
CciI	TCATGA	2 cut(s)	1189, 2677
CfoI	GCGC	1 cut(s)	312
Cfr10I	RCCGGY	1 cut(s)	1915
Cfr13I	GGNCC	5 cut(s)	136, 870, 1655, 1757, 2582
ClaI	ATCGAT	1 cut(s)	1212
CseI	GACGC	1 cut(s)	2180
CsiI	ACCWGGT	1 cut(s)	908
Csp6I	GTAC	4 cut(s)	184, 1120, 1334, 1673
CspAI	ACCGGT	1 cut(s)	1915
CviQI	GTAC	4 cut(s)	184, 1120, 1334, 1673
DriI	GACNNNNNGTC	1 cut(s)	2135
Eam1104I	CTCTTC	5 cut(s)	1133, 2229, 2343, 2450, 2557
Eam1105I	GACNNNNNGTC	1 cut(s)	2135
EarI	CTCTTC	5 cut(s)	1133, 2229, 2343, 2450, 2557
Eco130I	CCWWGG	4 cut(s)	100, 873, 1815, 2140
Eco147I	AGGCCT	1 cut(s)	542
Eco24I	GRGCYC	1 cut(s)	2552
Eco47I	GGWCC	4 cut(s)	136, 870, 1655, 2582
Eco47III	AGCGCT	1 cut(s)	311
Eco57I	CTGAAG	4 cut(s)	2140, 2324, 2456, 2538
Eco88I	CYCGRG	2 cut(s)	367, 1366
EcoRI	GAATTC	5 cut(s)	57, 380, 479, 1158, 2266
EcoRII	CCWGG	6 cut(s)	210, 537, 600, 762, 908, 2450
EcoT14I	CCWWGG	4 cut(s)	100, 873, 1815, 2140
EcoT22I	ATGCAT	2 cut(s)	408, 2689
EcoT38I	GRGCYC	1 cut(s)	2552
ErhI	CCWWGG	4 cut(s)	100, 873, 1815, 2140
FalI	AAGNNNNNCTT	4 cut(s)	1262, 1294, 2429, 2461
FaqI	GGGAC	3 cut(s)	95, 122, 1910
FbaI	TGATCA	2 cut(s)	178, 442
FblI	GTMKAC	2 cut(s)	1045, 1410
Fnu4HI	GCNGC	4 cut(s)	417, 822, 825, 2671
FokI	GGATG	9 cut(s)	174, 194, 516, 779, 1146, 1157, 1630, 2036, 2261
FriOI	GRGCYC	1 cut(s)	2552
Fsp4HI	GCNGC	4 cut(s)	417, 822, 825, 2671
GlaI	GCGC	1 cut(s)	311
GluI	GCNGC	4 cut(s)	417, 822, 825, 2671
HaeII	RGCGCY	1 cut(s)	313
HaeIII	GGCC	2 cut(s)	542, 1758
HapII	CCGG	2 cut(s)	114, 1916
HgaI	GACGC	1 cut(s)	2180
HhaI	GCGC	1 cut(s)	312
Hin1I	GRCGYC	2 cut(s)	156, 2191
Hin6I	GCGC	1 cut(s)	310
HinP1I	GCGC	1 cut(s)	310
HincII	GTYRAC	5 cut(s)	124, 374, 433, 946, 1437
HindII	GTYRAC	5 cut(s)	124, 374, 433, 946, 1437
HindIII	AAGCTT	1 cut(s)	1519
HpaI	GTTAAC	1 cut(s)	124
HpaII	CCGG	2 cut(s)	114, 1916
HphI	GGTGA	2 cut(s)	1618, 2211
Hpy99I	CGWCG	4 cut(s)	155, 158, 161, 2196
HpyAV	CCTTC	4 cut(s)	37, 729, 1370, 2537
HpyCH4III	ACNGT	7 cut(s)	175, 1041, 1153, 1327, 1645, 2020, 2104
HpyCH4IV	ACGT	5 cut(s)	126, 156, 288, 1651, 1832
HpyF10VI	GCNNNNNNNGC	4 cut(s)	260, 539, 1379, 2440
HpySE526I	ACGT	5 cut(s)	126, 156, 288, 1651, 1832
Hsp92I	GRCGYC	2 cut(s)	156, 2191
HspAI	GCGC	1 cut(s)	310
Ksp22I	TGATCA	2 cut(s)	178, 442
KspAI	GTTAAC	1 cut(s)	124
LguI	GCTCTTC	2 cut(s)	2450, 2557
LmnI	GCTCC	6 cut(s)	200, 554, 1594, 1729, 2344, 2653
Lsp1109I	GCAGC	3 cut(s)	428, 808, 811
LweI	GCATC	6 cut(s)	322, 469, 1007, 1082, 2031, 2674
MabI	ACCWGGT	1 cut(s)	908
MaeII	ACGT	5 cut(s)	126, 156, 288, 1651, 1832
MaeIII	GTNAC	6 cut(s)	349, 487, 1081, 1624, 1795, 1998
MflI	RGATCY	2 cut(s)	1010, 2009
MhlI	GDGCHC	2 cut(s)	559, 2552
MlyI	GAGTC	9 cut(s)	80, 233, 484, 566, 640, 670, 1001, 2063, 2145
MmeI	TCCRAC	6 cut(s)	34, 139, 216, 222, 544, 1316
Mph1103I	ATGCAT	2 cut(s)	408, 2689
MroXI	GAANNNNTTC	2 cut(s)	1536, 2444
MseI	TTAA	8 cut(s)	123, 471, 741, 807, 1253, 1269, 1686, 1941
MslI	CAYNNNNRTG	5 cut(s)	401, 1616, 1814, 2679, 2682
MspCI	CTTAAG	1 cut(s)	1252
MspI	CCGG	2 cut(s)	114, 1916
MspR9I	CCNGG	7 cut(s)	114, 212, 539, 602, 764, 910, 2452
Mva1269I	GAATGC	1 cut(s)	2126
MvaI	CCWGG	6 cut(s)	212, 539, 602, 764, 910, 2452
MvnI	CGCG	1 cut(s)	1878
MwoI	GCNNNNNNNGC	4 cut(s)	260, 539, 1379, 2440
NciI	CCSGG	1 cut(s)	114
NcoI	CCATGG	2 cut(s)	873, 1815
NheI	GCTAGC	1 cut(s)	1388
NlaIV	GGNNCC	6 cut(s)	138, 196, 914, 1372, 1590, 2011
NmeAIII	GCCGAG	2 cut(s)	94, 2110
NmuCI	GTSAC	4 cut(s)	349, 487, 1624, 1998
NruI	TCGCGA	1 cut(s)	1878
NsiI	ATGCAT	2 cut(s)	408, 2689
NspI	RCATGY	3 cut(s)	400, 1736, 1960
PagI	TCATGA	2 cut(s)	1189, 2677
PaqCI	CACCTGC	1 cut(s)	1373
PceI	AGGCCT	1 cut(s)	542
PciSI	GCTCTTC	2 cut(s)	2450, 2557
PctI	GAATGC	1 cut(s)	2126
PdmI	GAANNNNTTC	2 cut(s)	1536, 2444
PflFI	GACNNNGTC	3 cut(s)	155, 359, 1151
PflMI	CCANNNNNTGG	2 cut(s)	500, 1343
PfoI	TCCNGGA	1 cut(s)	762
PinAI	ACCGGT	1 cut(s)	1915
PkrI	GCNGC	4 cut(s)	418, 823, 826, 2672
Ple19I	CGATCG	1 cut(s)	1996
PleI	GAGTC	9 cut(s)	79, 233, 484, 566, 639, 669, 1000, 2063, 2144
PpsI	GAGTC	9 cut(s)	79, 233, 484, 566, 639, 669, 1000, 2063, 2144
Ppu21I	YACGTR	1 cut(s)	1652
PshAI	GACNNNNGTC	1 cut(s)	630
PshBI	ATTAAT	2 cut(s)	1686, 1941
PsiI	TTATAA	2 cut(s)	1560, 2333
Psp1406I	AACGTT	1 cut(s)	1832
Psp6I	CCWGG	6 cut(s)	210, 537, 600, 762, 908, 2450
PspGI	CCWGG	6 cut(s)	210, 537, 600, 762, 908, 2450
PspN4I	GGNNCC	6 cut(s)	138, 196, 914, 1372, 1590, 2011
PspPI	GGNCC	5 cut(s)	136, 870, 1655, 1757, 2582
PstI	CTGCAG	1 cut(s)	829
PsuI	RGATCY	2 cut(s)	1010, 2009
PsyI	GACNNNGTC	3 cut(s)	155, 359, 1151
PvuI	CGATCG	1 cut(s)	1996
RruI	TCGCGA	1 cut(s)	1878
RsaI	GTAC	4 cut(s)	185, 1121, 1335, 1674
RsaNI	GTAC	4 cut(s)	184, 1120, 1334, 1673
RseI	CAYNNNNRTG	5 cut(s)	401, 1616, 1814, 2679, 2682
SapI	GCTCTTC	2 cut(s)	2450, 2557
SaqAI	TTAA	8 cut(s)	123, 471, 741, 807, 1253, 1269, 1686, 1941
SatI	GCNGC	4 cut(s)	417, 822, 825, 2671
Sau96I	GGNCC	5 cut(s)	136, 870, 1655, 1757, 2582
ScaI	AGTACT	1 cut(s)	185
SchI	GAGTC	9 cut(s)	80, 233, 484, 566, 640, 670, 1001, 2063, 2145
ScrFI	CCNGG	7 cut(s)	114, 212, 539, 602, 764, 910, 2452
SduI	GDGCHC	2 cut(s)	559, 2552
SexAI	ACCWGGT	1 cut(s)	908
SfaNI	GCATC	6 cut(s)	322, 469, 1007, 1082, 2031, 2674
SfcI	CTRYAG	2 cut(s)	825, 1882
SinI	GGWCC	4 cut(s)	136, 870, 1655, 2582
SmiMI	CAYNNNNRTG	5 cut(s)	401, 1616, 1814, 2679, 2682
SmlI	CTYRAG	3 cut(s)	635, 1252, 2071
SmoI	CTYRAG	3 cut(s)	635, 1252, 2071
SpeI	ACTAGT	2 cut(s)	961, 2602
SseBI	AGGCCT	1 cut(s)	542
SsiI	CCGC	3 cut(s)	2632, 2668, 2671
StuI	AGGCCT	1 cut(s)	542
StyD4I	CCNGG	7 cut(s)	112, 210, 537, 600, 762, 908, 2450
StyI	CCWWGG	4 cut(s)	100, 873, 1815, 2140
TaaI	ACNGT	7 cut(s)	175, 1041, 1153, 1327, 1645, 2020, 2104
TaiI	ACGT	5 cut(s)	129, 159, 291, 1654, 1835
TaqI	TCGA	6 cut(s)	483, 570, 589, 850, 1212, 1992
TaqII	GACCGA	2 cut(s)	40, 1986
TatI	WGTACW	3 cut(s)	183, 1119, 1672
TauI	GCSGC	1 cut(s)	2673
Tru1I	TTAA	8 cut(s)	123, 471, 741, 807, 1253, 1269, 1686, 1941
Tru9I	TTAA	8 cut(s)	123, 471, 741, 807, 1253, 1269, 1686, 1941
TscAI	CASTG	5 cut(s)	358, 1046, 1816, 1846, 2025
TseFI	GTSAC	4 cut(s)	349, 487, 1624, 1998
TseI	GCWGC	3 cut(s)	416, 821, 824
Tsp45I	GTSAC	4 cut(s)	349, 487, 1624, 1998
TspGWI	ACGGA	3 cut(s)	905, 1321, 2576
TspRI	CASTG	5 cut(s)	358, 1046, 1816, 1846, 2025
Tth111I	GACNNNGTC	3 cut(s)	155, 359, 1151
Van91I	CCANNNNNTGG	2 cut(s)	500, 1343
Vha464I	CTTAAG	1 cut(s)	1252
VpaK11BI	GGWCC	4 cut(s)	136, 870, 1655, 2582
VspI	ATTAAT	2 cut(s)	1686, 1941
XbaI	TCTAGA	1 cut(s)	1231
XceI	RCATGY	3 cut(s)	400, 1736, 1960
XcmI	CCANNNNNNNNNTGG	2 cut(s)	501, 1919
XmaJI	CCTAGG	1 cut(s)	2140
XmiI	GTMKAC	2 cut(s)	1045, 1410
XmnI	GAANNNNTTC	2 cut(s)	1536, 2444
ZraI	GACGTC	1 cut(s)	157
ZrmI	AGTACT	1 cut(s)	185
Zsp2I	ATGCAT	2 cut(s)	408, 2689

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.