FvH4_6g04801

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002274
Physical Location & Seq
Reverse (-)
2650234 .. 2651207
974 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g04801.t1

Sequence Viewer

Length: 666 bp
ATGGGAGGTTCTGCCTCGAAAAAGCTATCGAGCCGATCAGAAAGGTCCTGCAGCAGCAGGAATGGCTACAGTCCAGAATGTTTTGATTATGTTTTCGACTGCTTATCCCCACGATGTTCATACTTGAATGAACCAACTGCAGATGAACCTAGCGCTTCTTCTTCTTCTTATTCCGAACCAGGTCGTGACACTGCAAATTCACACCCCCTGCCATCAGCTCGAGTACAACCATTTTCATACGAAGATGAAGAAGAGAAGCAGATCGATATGGCGATTACGGCATCTCTGGAAGTACCAAACTTCCGTAGGGAATCTTCTACCTCGAGTATGAGCTCTAGCTCTGCATCCGATGACGAAGAAGAGCGGCAGATTGATACGGCGATTTGGGCCTCTCTGAACCCTAGCTATGGTGAAGAATGGTCATCTGAGTCTGAGAGTGGTAGTAGCTCTTCTTCTTCTTCCCAGCCAAACGAAGATTCAAATCATTTCAGTGGCGAATTAGGAGGATGTGGGATATGTTTCGAAGTCATTTCAATGCCAGCAATATGTGACCCCTGTGGGCATGTGAGCTGCCACGATTGTTTGCAACGGATTCAGAGCAGTACTGCTAGTAGGTGTCCTCGTTGTCGTGGAGGAATCACCAAAGTGATGAGGATTTATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

222

Amino Acids

24.0

Weight (kDa)

4.62

Isoelectric Point (pI)

81.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 170 - 216 3.6e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 170 - 209 8.6e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 170 - 207 1e-05 RING-type zinc-finger
zf-C3HC4 PF00097 170 - 209 9.5e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 364
AciI CCGC 1 cut(s) 364
AcsI RAATTY 1 cut(s) 196
AfaI GTAC 3 cut(s) 225, 294, 604
AfeI AGCGCT 1 cut(s) 154
AfiI CCNNNNNNNGG 1 cut(s) 407
AgsI TTSAA 3 cut(s) 127, 480, 534
AjnI CCWGG 1 cut(s) 178
AjuI GAANNNNNNNTTGG 2 cut(s) 460, 492
AleI CACNNNNGTG 1 cut(s) 644
AluBI AGCT 7 cut(s) 25, 218, 333, 339, 405, 447, 570
AluI AGCT 7 cut(s) 25, 218, 333, 339, 405, 447, 570
Alw21I GWGCWC 1 cut(s) 335
Ama87I CYCGRG 2 cut(s) 219, 322
Aor51HI AGCGCT 1 cut(s) 154
AoxI GGCC 1 cut(s) 387
ApeKI GCWGC 3 cut(s) 51, 54, 570
ApoI RAATTY 1 cut(s) 196
AspLEI GCGC 1 cut(s) 155
AspS9I GGNCC 2 cut(s) 45, 387
AsuHPI GGTGA 2 cut(s) 422, 631
AsuII TTCGAA 1 cut(s) 522
AvaI CYCGRG 2 cut(s) 219, 322
AvaII GGWCC 1 cut(s) 45
BanII GRGCYC 1 cut(s) 335
BarI GAAGNNNNNNTAC 6 cut(s) 298, 330, 433, 436, 465, 468
Bbv12I GWGCWC 1 cut(s) 335
BbvI GCAGC 3 cut(s) 63, 66, 557
BccI CCATC 1 cut(s) 220
BceAI ACGGC 2 cut(s) 294, 393
BciT130I CCWGG 1 cut(s) 180
BfaI CTAG 4 cut(s) 150, 336, 402, 609
BfmI CTRYAG 3 cut(s) 49, 67, 138
BfoI RGCGCY 1 cut(s) 156
BisI GCNGC 4 cut(s) 52, 55, 365, 571
BlsI GCNGC 4 cut(s) 53, 56, 366, 572
BmcAI AGTACT 1 cut(s) 604
Bme1390I CCNGG 1 cut(s) 180
Bme18I GGWCC 1 cut(s) 45
BmeT110I CYCGRG 2 cut(s) 219, 322
BmgT120I GGNCC 2 cut(s) 45, 387
BmrFI CCNGG 1 cut(s) 180
BmsI GCATC 2 cut(s) 290, 353
Bpu14I TTCGAA 1 cut(s) 522
Bsa29I ATCGAT 1 cut(s) 264
BsaBI GATNNNNATC 1 cut(s) 480
Bsc4I CCNNNNNNNGG 1 cut(s) 407
Bse8I GATNNNNATC 1 cut(s) 480
BseBI CCWGG 1 cut(s) 180
BseCI ATCGAT 1 cut(s) 264
BseGI GGATG 2 cut(s) 344, 512
BseJI GATNNNNATC 1 cut(s) 480
BseLI CCNNNNNNNGG 1 cut(s) 407
BseMII CTCAG 2 cut(s) 417, 423
BseXI GCAGC 3 cut(s) 63, 66, 557
BseYI CCCAGC 1 cut(s) 462
BshFI GGCC 1 cut(s) 389
BshVI ATCGAT 1 cut(s) 264
BsiHKAI GWGCWC 1 cut(s) 335
BsiHKCI CYCGRG 2 cut(s) 219, 322
BslI CCNNNNNNNGG 1 cut(s) 407
BsnI GGCC 1 cut(s) 389
BsoBI CYCGRG 2 cut(s) 219, 322
Bsp119I TTCGAA 1 cut(s) 522
Bsp1286I GDGCHC 1 cut(s) 335
Bsp143I GATC 2 cut(s) 35, 261
BspACI CCGC 1 cut(s) 364
BspANI GGCC 1 cut(s) 389
BspCNI CTCAG 2 cut(s) 418, 424
BspDI ATCGAT 1 cut(s) 264
BspMAI CTGCAG 2 cut(s) 53, 142
BspQI GCTCTTC 2 cut(s) 354, 454
BspT104I TTCGAA 1 cut(s) 522
BsrBI CCGCTC 1 cut(s) 364
BssMI GATC 2 cut(s) 35, 261
Bst2UI CCWGG 1 cut(s) 180
Bst4CI ACNGT 1 cut(s) 71
Bst6I CTCTTC 3 cut(s) 246, 354, 454
BstBI TTCGAA 1 cut(s) 522
BstC8I GCNNGC 1 cut(s) 540
BstDEI CTNAG 2 cut(s) 426, 432
BstF5I GGATG 2 cut(s) 344, 512
BstH2I RGCGCY 1 cut(s) 156
BstHHI GCGC 1 cut(s) 155
BstKTI GATC 2 cut(s) 38, 264
BstMBI GATC 2 cut(s) 35, 261
BstMWI GCNNNNNNNGC 3 cut(s) 63, 278, 386
BstNI CCWGG 1 cut(s) 180
BstNSI RCATGY 1 cut(s) 566
BstSCI CCNGG 1 cut(s) 178
BstSFI CTRYAG 3 cut(s) 49, 67, 138
BstV1I GCAGC 3 cut(s) 63, 66, 557
Bsu15I ATCGAT 1 cut(s) 264
BsuRI GGCC 1 cut(s) 389
BsuTUI ATCGAT 1 cut(s) 264
BtsCI GGATG 2 cut(s) 344, 512
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 2 cut(s) 189, 496
Cac8I GCNNGC 1 cut(s) 540
CfoI GCGC 1 cut(s) 155
Cfr13I GGNCC 2 cut(s) 45, 387
ClaI ATCGAT 1 cut(s) 264
CsiI ACCWGGT 1 cut(s) 178
Csp6I GTAC 3 cut(s) 224, 293, 603
CviAII CATG 1 cut(s) 563
CviQI GTAC 3 cut(s) 224, 293, 603
DdeI CTNAG 2 cut(s) 426, 432
DpnI GATC 2 cut(s) 37, 263
DpnII GATC 2 cut(s) 35, 261
Eam1104I CTCTTC 3 cut(s) 246, 354, 454
EarI CTCTTC 3 cut(s) 246, 354, 454
Ecl136II GAGCTC 1 cut(s) 333
Eco24I GRGCYC 1 cut(s) 335
Eco47I GGWCC 1 cut(s) 45
Eco47III AGCGCT 1 cut(s) 154
Eco53kI GAGCTC 1 cut(s) 333
Eco88I CYCGRG 2 cut(s) 219, 322
EcoICRI GAGCTC 1 cut(s) 333
EcoO109I RGGNCCY 1 cut(s) 45
EcoRII CCWGG 1 cut(s) 178
EcoT38I GRGCYC 1 cut(s) 335
FaeI CATG 1 cut(s) 566
FatI CATG 1 cut(s) 562
Fnu4HI GCNGC 4 cut(s) 52, 55, 365, 571
FokI GGATG 2 cut(s) 331, 519
FriOI GRGCYC 1 cut(s) 335
Fsp4HI GCNGC 4 cut(s) 52, 55, 365, 571
FspBI CTAG 4 cut(s) 150, 336, 402, 609
GlaI GCGC 1 cut(s) 154
GluI GCNGC 4 cut(s) 52, 55, 365, 571
GsaI CCCAGC 1 cut(s) 466
HaeII RGCGCY 1 cut(s) 156
HaeIII GGCC 1 cut(s) 389
HhaI GCGC 1 cut(s) 155
Hin1II CATG 1 cut(s) 566
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HinfI GANTC 5 cut(s) 311, 428, 476, 592, 636
HphI GGTGA 2 cut(s) 422, 631
Hpy188I TCNGA 7 cut(s) 40, 175, 349, 396, 427, 433, 597
Hpy188III TCNNGA 3 cut(s) 74, 185, 287
HpyCH4III ACNGT 1 cut(s) 71
HpyCH4V TGCA 5 cut(s) 51, 140, 194, 344, 586
HpyF10VI GCNNNNNNNGC 3 cut(s) 63, 278, 386
HpyF3I CTNAG 2 cut(s) 426, 432
Hsp92II CATG 1 cut(s) 566
HspAI GCGC 1 cut(s) 153
Kzo9I GATC 2 cut(s) 35, 261
LguI GCTCTTC 2 cut(s) 354, 454
Lsp1109I GCAGC 3 cut(s) 63, 66, 557
LweI GCATC 2 cut(s) 290, 353
MabI ACCWGGT 1 cut(s) 178
MaeI CTAG 4 cut(s) 150, 336, 402, 609
MaeIII GTNAC 2 cut(s) 185, 548
MalI GATC 2 cut(s) 37, 263
MbiI CCGCTC 1 cut(s) 364
MboI GATC 2 cut(s) 35, 261
MhlI GDGCHC 1 cut(s) 335
MluCI AATT 2 cut(s) 196, 497
MlyI GAGTC 1 cut(s) 437
MnlI CCTC 7 cut(s) 25, 331, 400, 497, 626, 630, 645
MslI CAYNNNNRTG 3 cut(s) 489, 533, 644
MspR9I CCNGG 1 cut(s) 180
MvaI CCWGG 1 cut(s) 180
MwoI GCNNNNNNNGC 3 cut(s) 63, 278, 386
NdeII GATC 2 cut(s) 35, 261
NlaIII CATG 1 cut(s) 566
NmuCI GTSAC 2 cut(s) 185, 548
NspI RCATGY 1 cut(s) 566
NspV TTCGAA 1 cut(s) 522
OliI CACNNNNGTG 1 cut(s) 644
PaeR7I CTCGAG 2 cut(s) 219, 322
PciSI GCTCTTC 2 cut(s) 354, 454
PfeI GAWTC 4 cut(s) 311, 476, 592, 636
PkrI GCNGC 4 cut(s) 53, 56, 366, 572
PleI GAGTC 1 cut(s) 436
PpsI GAGTC 1 cut(s) 436
PpuMI RGGWCCY 1 cut(s) 45
Psp124BI GAGCTC 1 cut(s) 335
Psp5II RGGWCCY 1 cut(s) 45
Psp6I CCWGG 1 cut(s) 178
PspFI CCCAGC 1 cut(s) 462
PspGI CCWGG 1 cut(s) 178
PspPI GGNCC 2 cut(s) 45, 387
PspPPI RGGWCCY 1 cut(s) 45
PspXI VCTCGAGB 2 cut(s) 219, 322
PstI CTGCAG 2 cut(s) 53, 142
RsaI GTAC 3 cut(s) 225, 294, 604
RsaNI GTAC 3 cut(s) 224, 293, 603
RseI CAYNNNNRTG 3 cut(s) 489, 533, 644
SacI GAGCTC 1 cut(s) 335
SapI GCTCTTC 2 cut(s) 354, 454
SatI GCNGC 4 cut(s) 52, 55, 365, 571
Sau3AI GATC 2 cut(s) 35, 261
Sau96I GGNCC 2 cut(s) 45, 387
ScaI AGTACT 1 cut(s) 604
SchI GAGTC 1 cut(s) 437
ScrFI CCNGG 1 cut(s) 180
SduI GDGCHC 1 cut(s) 335
SexAI ACCWGGT 1 cut(s) 178
SfaNI GCATC 2 cut(s) 290, 353
SfcI CTRYAG 3 cut(s) 49, 67, 138
Sfr274I CTCGAG 2 cut(s) 219, 322
SfuI TTCGAA 1 cut(s) 522
SinI GGWCC 1 cut(s) 45
SlaI CTCGAG 2 cut(s) 219, 322
SmiMI CAYNNNNRTG 3 cut(s) 489, 533, 644
SmlI CTYRAG 2 cut(s) 219, 322
SmoI CTYRAG 2 cut(s) 219, 322
Sse9I AATT 2 cut(s) 196, 497
SsiI CCGC 1 cut(s) 364
SspMI CTAG 4 cut(s) 150, 336, 402, 609
SstI GAGCTC 1 cut(s) 335
StyD4I CCNGG 1 cut(s) 178
TaaI ACNGT 1 cut(s) 71
TaqI TCGA 7 cut(s) 17, 29, 96, 220, 264, 323, 522
TasI AATT 2 cut(s) 196, 497
TatI WGTACW 2 cut(s) 223, 602
TauI GCSGC 1 cut(s) 367
TfiI GAWTC 4 cut(s) 311, 476, 592, 636
TscAI CASTG 2 cut(s) 196, 496
TseFI GTSAC 2 cut(s) 185, 548
TseI GCWGC 3 cut(s) 51, 54, 570
Tsp45I GTSAC 2 cut(s) 185, 548
TspDTI ATGAA 5 cut(s) 108, 144, 159, 225, 261
TspGWI ACGGA 2 cut(s) 293, 604
TspRI CASTG 2 cut(s) 196, 496
VpaK11BI GGWCC 1 cut(s) 45
XapI RAATTY 1 cut(s) 196
XceI RCATGY 1 cut(s) 566
XhoI CTCGAG 2 cut(s) 219, 322
XspI CTAG 4 cut(s) 150, 336, 402, 609
ZrmI AGTACT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.