Rh4AG163500

BONZAI 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002274
Physical Location & Seq
Forward (+)
41405024 .. 41407730
2707 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG163500.1

Sequence Viewer

Length: 357 bp
ATGGGAGGGTGTTTTTCCGACGTGAAAGGTGGCAAGCAGGCCGTGGGAGGGGCCCATCATCAAGAGAGGCCCATCAACAGGGACGCTGACTCAAACGACGCCGTTGAGCTCTTTAACCGCTCCCGAGGCGTTTTTCCACTGCACACTTCGCTCGAGTTATCGCTGTCAGCAACTAACTTGCTTGATCGTGACATTATGTCAAAGAGTGATCCCATGGTTGTGGTTTATGTGAAGAAAGGACATGGTAAGCTGGAGGAACTAGGGAGAACTGAAGTAATACTGAATAGTTTGAATCCAGTGTGGATTGAGAAAATTACAGTTTCATATCAGTTTGAGGTTGTGCAGCCATTGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009987 GO:0010118 GO:0010941 GO:0016020 GO:0044464 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0060548 GO:0065007 GO:0071944 GO:0090332
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Copine

Protein Analysis

118

Amino Acids

13.06

Weight (kDa)

6.05

Isoelectric Point (pI)

29.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 55 - 113 6e-10 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 120
AciI CCGC 1 cut(s) 118
AclWI GGATC 1 cut(s) 203
AcuI CTGAAG 1 cut(s) 291
AcyI GRCGYC 1 cut(s) 99
AfiI CCNNNNNNNGG 2 cut(s) 48, 78
AgsI TTSAA 1 cut(s) 292
AhdI GACNNNNNGTC 1 cut(s) 196
AjiI CACGTC 1 cut(s) 22
AluBI AGCT 2 cut(s) 109, 250
AluI AGCT 2 cut(s) 109, 250
Alw21I GWGCWC 1 cut(s) 111
AlwI GGATC 1 cut(s) 203
Ama87I CYCGRG 2 cut(s) 123, 152
AoxI GGCC 3 cut(s) 39, 51, 68
ApaI GGGCCC 1 cut(s) 55
ApeKI GCWGC 1 cut(s) 343
AspS9I GGNCC 3 cut(s) 51, 52, 69
AvaI CYCGRG 2 cut(s) 123, 152
BaeGI GKGCMC 1 cut(s) 55
BanII GRGCYC 2 cut(s) 55, 111
Bbv12I GWGCWC 1 cut(s) 111
BccI CCATC 2 cut(s) 63, 80
BceAI ACGGC 2 cut(s) 26, 86
BfaI CTAG 1 cut(s) 260
BisI GCNGC 1 cut(s) 344
BlsI GCNGC 1 cut(s) 345
BmeRI GACNNNNNGTC 1 cut(s) 196
BmeT110I CYCGRG 2 cut(s) 123, 152
BmgBI CACGTC 1 cut(s) 22
BmgT120I GGNCC 3 cut(s) 51, 52, 69
BmiI GGNNCC 2 cut(s) 52, 53
BpmI CTGGAG 1 cut(s) 272
BsaHI GRCGYC 1 cut(s) 99
BsaJI CCNNGG 3 cut(s) 42, 124, 213
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 78
Bse1I ACTGG 1 cut(s) 296
BseDI CCNNGG 3 cut(s) 42, 124, 213
BseLI CCNNNNNNNGG 2 cut(s) 48, 78
BseNI ACTGG 1 cut(s) 296
BseSI GKGCMC 1 cut(s) 55
BsgI GTGCAG 1 cut(s) 125
BshFI GGCC 3 cut(s) 41, 53, 70
BsiHKAI GWGCWC 1 cut(s) 111
BsiHKCI CYCGRG 2 cut(s) 123, 152
BslFI GGGAC 1 cut(s) 95
BslI CCNNNNNNNGG 2 cut(s) 48, 78
BsmFI GGGAC 1 cut(s) 95
BsnI GGCC 3 cut(s) 41, 53, 70
BsoBI CYCGRG 2 cut(s) 123, 152
Bsp120I GGGCCC 1 cut(s) 51
Bsp1286I GDGCHC 2 cut(s) 55, 111
Bsp143I GATC 2 cut(s) 184, 208
Bsp19I CCATGG 1 cut(s) 213
BspACI CCGC 1 cut(s) 118
BspANI GGCC 3 cut(s) 41, 53, 70
BspLI GGNNCC 2 cut(s) 52, 53
BspPI GGATC 1 cut(s) 203
BsrBI CCGCTC 1 cut(s) 120
BsrI ACTGG 1 cut(s) 296
BssECI CCNNGG 3 cut(s) 42, 124, 213
BssMI GATC 2 cut(s) 184, 208
BssNI GRCGYC 1 cut(s) 99
BssT1I CCWWGG 1 cut(s) 213
Bst4CI ACNGT 1 cut(s) 319
BstACI GRCGYC 1 cut(s) 99
BstC8I GCNNGC 2 cut(s) 35, 39
BstDSI CCRYGG 2 cut(s) 42, 213
BstKTI GATC 2 cut(s) 187, 211
BstMBI GATC 2 cut(s) 184, 208
BstMWI GCNNNNNNNGC 2 cut(s) 126, 148
BstSLI GKGCMC 1 cut(s) 55
BstXI CCANNNNNNTGG 1 cut(s) 220
BsuRI GGCC 3 cut(s) 41, 53, 70
BtgI CCRYGG 2 cut(s) 42, 213
BtrI CACGTC 1 cut(s) 22
BtsI GCAGTG 1 cut(s) 137
BtsIMutI CAGTG 2 cut(s) 137, 303
Cac8I GCNNGC 2 cut(s) 35, 39
Cfr13I GGNCC 3 cut(s) 51, 52, 69
CseI GACGC 2 cut(s) 92, 107
CviAII CATG 2 cut(s) 214, 242
CviJI RGCY 6 cut(s) 41, 53, 70, 109, 250, 346
CviKI_1 RGCY 6 cut(s) 41, 53, 70, 109, 250, 346
DpnI GATC 2 cut(s) 186, 210
DpnII GATC 2 cut(s) 184, 208
DriI GACNNNNNGTC 1 cut(s) 196
Eam1105I GACNNNNNGTC 1 cut(s) 196
Ecl136II GAGCTC 1 cut(s) 109
Eco130I CCWWGG 1 cut(s) 213
Eco24I GRGCYC 2 cut(s) 55, 111
Eco53kI GAGCTC 1 cut(s) 109
Eco57I CTGAAG 1 cut(s) 291
Eco88I CYCGRG 2 cut(s) 123, 152
EcoICRI GAGCTC 1 cut(s) 109
EcoO109I RGGNCCY 1 cut(s) 51
EcoT14I CCWWGG 1 cut(s) 213
EcoT38I GRGCYC 2 cut(s) 55, 111
ErhI CCWWGG 1 cut(s) 213
FaeI CATG 2 cut(s) 217, 245
FaiI YATR 5 cut(s) 197, 215, 228, 243, 325
FaqI GGGAC 1 cut(s) 95
FatI CATG 2 cut(s) 213, 241
Fnu4HI GCNGC 1 cut(s) 344
FriOI GRGCYC 2 cut(s) 55, 111
Fsp4HI GCNGC 1 cut(s) 344
FspBI CTAG 1 cut(s) 260
GluI GCNGC 1 cut(s) 344
GsuI CTGGAG 1 cut(s) 272
HaeIII GGCC 3 cut(s) 41, 53, 70
HgaI GACGC 2 cut(s) 92, 107
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 2 cut(s) 217, 245
HinfI GANTC 2 cut(s) 89, 292
Hpy188I TCNGA 1 cut(s) 19
Hpy188III TCNNGA 3 cut(s) 62, 123, 188
Hpy99I CGWCG 2 cut(s) 23, 101
HpyCH4III ACNGT 1 cut(s) 319
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 2 cut(s) 142, 343
HpyF10VI GCNNNNNNNGC 2 cut(s) 126, 148
HpySE526I ACGT 1 cut(s) 21
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 2 cut(s) 217, 245
Kzo9I GATC 2 cut(s) 184, 208
LmnI GCTCC 1 cut(s) 125
LpnPI CCDG 4 cut(s) 23, 64, 236, 309
MaeI CTAG 1 cut(s) 260
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 1 cut(s) 188
MalI GATC 2 cut(s) 186, 210
MbiI CCGCTC 1 cut(s) 120
MboI GATC 2 cut(s) 184, 208
MboII GAAGA 1 cut(s) 244
MhlI GDGCHC 2 cut(s) 55, 111
MluCI AATT 1 cut(s) 312
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 1 cut(s) 42
MnlI CCTC 5 cut(s) 41, 60, 119, 247, 328
MseI TTAA 1 cut(s) 114
MslI CAYNNNNRTG 1 cut(s) 218
MwoI GCNNNNNNNGC 2 cut(s) 126, 148
NcoI CCATGG 1 cut(s) 213
NdeII GATC 2 cut(s) 184, 208
NlaIII CATG 2 cut(s) 217, 245
NlaIV GGNNCC 2 cut(s) 52, 53
NmuCI GTSAC 1 cut(s) 188
PaeR7I CTCGAG 1 cut(s) 152
PfeI GAWTC 1 cut(s) 292
PkrI GCNGC 1 cut(s) 345
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
Psp124BI GAGCTC 1 cut(s) 111
PspN4I GGNNCC 2 cut(s) 52, 53
PspOMI GGGCCC 1 cut(s) 51
PspPI GGNCC 3 cut(s) 51, 52, 69
PspXI VCTCGAGB 1 cut(s) 152
RseI CAYNNNNRTG 1 cut(s) 218
SacI GAGCTC 1 cut(s) 111
SaqAI TTAA 1 cut(s) 114
SatI GCNGC 1 cut(s) 344
Sau3AI GATC 2 cut(s) 184, 208
Sau96I GGNCC 3 cut(s) 51, 52, 69
SchI GAGTC 1 cut(s) 83
SduI GDGCHC 2 cut(s) 55, 111
SetI ASST 5 cut(s) 24, 31, 111, 252, 339
Sfr274I CTCGAG 1 cut(s) 152
SlaI CTCGAG 1 cut(s) 152
SmiMI CAYNNNNRTG 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 152
SmoI CTYRAG 1 cut(s) 152
Sse9I AATT 1 cut(s) 312
SsiI CCGC 1 cut(s) 118
SspMI CTAG 1 cut(s) 260
SstI GAGCTC 1 cut(s) 111
StyI CCWWGG 1 cut(s) 213
TaaI ACNGT 1 cut(s) 319
TaiI ACGT 1 cut(s) 24
TaqI TCGA 1 cut(s) 153
TasI AATT 1 cut(s) 312
TfiI GAWTC 1 cut(s) 292
Tru1I TTAA 1 cut(s) 114
Tru9I TTAA 1 cut(s) 114
TscAI CASTG 2 cut(s) 144, 303
TseFI GTSAC 1 cut(s) 188
TseI GCWGC 1 cut(s) 343
Tsp45I GTSAC 1 cut(s) 188
TspDTI ATGAA 1 cut(s) 312
TspRI CASTG 2 cut(s) 144, 303
XhoI CTCGAG 1 cut(s) 152
XspI CTAG 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.