FvH4_7g13461
ERF Family

Thioesterase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002384
Physical Location & Seq
Reverse (-)
12073859 .. 12075482
1624 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g13461.t1

Sequence Viewer

Length: 546 bp
ATGAGGAAGATGGCAAACCCGTCGACTATTGAGACAGAGAGCTTTGCTTCCACAGCTTCAATCTCAAAGGACCTCCCTGAAAACCATGTCAAGCATCTCCTTCAGTTTCTCAAATTATCGGGTGTTTCTGGCCCTCTTCCAGATCACTGCAATACTAAAGACTTCTACTCCTACCTCGTTCTCGGCATCCTCAAGCCGCTCAACATTCATCGAGGCCGTGTCACCTGTCTTGTCTCCGTAAAGCCTGCTTTTTCTAACTTTTTTGGTGGATTCCATGGAGGAGCTATTGCTGCTGTTGCTGAGGCTGTGGCGTTCGCCACTGCAAGAACGGTTGTGGCTGAGGATAAGGAGCTTTTTCTAGGGGAACTAAGCATCGCTTACCTCTCTTCTGCTCCAAAGAATGCAGAGGTGATAGCTGATGGATCTGTAGTGCGGAGTGGAAGAAATCTGACTGTAATAGCACAGGAGTTCAAGCTGAAGGAAACCGGGAAATTGATCTACACCGCTCGTGCTACCTTCTATCACACGCCTGTTTCAAAATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4HBT

Protein Analysis

182

Amino Acids

19.57

Weight (kDa)

9.25

Isoelectric Point (pI)

29.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HBT PF03061 89 - 164 1.8e-09 Thioesterase superfamily
4HBT_3 PF13622 89 - 173 1e-05 Acyl-CoA thioesterase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 199, 506
AccI GTMKAC 1 cut(s) 23
AciI CCGC 3 cut(s) 197, 433, 504
AclWI GGATC 1 cut(s) 430
AcuI CTGAAG 2 cut(s) 86, 497
AgsI TTSAA 3 cut(s) 60, 472, 537
AluBI AGCT 6 cut(s) 42, 56, 284, 352, 416, 475
AluI AGCT 6 cut(s) 42, 56, 284, 352, 416, 475
Alw26I GTCTC 2 cut(s) 26, 238
AlwI GGATC 1 cut(s) 430
AoxI GGCC 2 cut(s) 130, 214
ApeKI GCWGC 1 cut(s) 290
AspS9I GGNCC 2 cut(s) 70, 131
AsuC2I CCSGG 1 cut(s) 487
AsuHPI GGTGA 2 cut(s) 214, 421
AvaII GGWCC 1 cut(s) 70
BauI CACGAG 1 cut(s) 507
BbvCI CCTCAGC 2 cut(s) 300, 339
BbvI GCAGC 1 cut(s) 277
BccI CCATC 2 cut(s) 4, 413
BceAI ACGGC 1 cut(s) 201
BcgI CGANNNNNNTGC 1 cut(s) 37
BcnI CCSGG 1 cut(s) 487
BcoDI GTCTC 2 cut(s) 26, 238
BfaI CTAG 1 cut(s) 359
BfmI CTRYAG 1 cut(s) 426
BisI GCNGC 2 cut(s) 197, 291
BlsI GCNGC 2 cut(s) 198, 292
Bme1390I CCNGG 1 cut(s) 487
Bme18I GGWCC 1 cut(s) 70
BmgT120I GGNCC 2 cut(s) 70, 131
BmrFI CCNGG 1 cut(s) 487
BmsI GCATC 3 cut(s) 103, 195, 381
Bpu10I CCTNAGC 2 cut(s) 300, 339
BpuEI CTTGAG 1 cut(s) 176
BpuMI CCSGG 1 cut(s) 487
BsaJI CCNNGG 1 cut(s) 274
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
BseDI CCNNGG 1 cut(s) 274
BseGI GGATG 1 cut(s) 186
BseMII CTCAG 2 cut(s) 291, 330
BseRI GAGGAG 1 cut(s) 294
BseXI GCAGC 1 cut(s) 277
BshFI GGCC 2 cut(s) 132, 216
BsiSI CCGG 1 cut(s) 486
BsmAI GTCTC 2 cut(s) 26, 238
BsmI GAATGC 1 cut(s) 406
BsnI GGCC 2 cut(s) 132, 216
Bsp143I GATC 3 cut(s) 142, 422, 495
Bsp19I CCATGG 1 cut(s) 274
BspACI CCGC 3 cut(s) 197, 433, 504
BspANI GGCC 2 cut(s) 132, 216
BspCNI CTCAG 2 cut(s) 292, 331
BspPI GGATC 1 cut(s) 430
BsrBI CCGCTC 2 cut(s) 199, 506
BssECI CCNNGG 1 cut(s) 274
BssMI GATC 3 cut(s) 142, 422, 495
BssSI CACGAG 1 cut(s) 507
BssT1I CCWWGG 1 cut(s) 274
Bst2BI CACGAG 1 cut(s) 507
Bst4CI ACNGT 2 cut(s) 331, 454
Bst6I CTCTTC 2 cut(s) 141, 391
BstC8I GCNNGC 1 cut(s) 246
BstDEI CTNAG 3 cut(s) 300, 339, 368
BstDSI CCRYGG 1 cut(s) 274
BstF5I GGATG 1 cut(s) 186
BstKTI GATC 3 cut(s) 145, 425, 498
BstMAI GTCTC 2 cut(s) 26, 238
BstMBI GATC 3 cut(s) 142, 422, 495
BstMWI GCNNNNNNNGC 3 cut(s) 53, 290, 296
BstSCI CCNGG 1 cut(s) 485
BstSFI CTRYAG 1 cut(s) 426
BstV1I GCAGC 1 cut(s) 277
BstX2I RGATCY 1 cut(s) 422
BstYI RGATCY 1 cut(s) 422
BsuRI GGCC 2 cut(s) 132, 216
BtgI CCRYGG 1 cut(s) 274
BtgZI GCGATG 1 cut(s) 358
BtsCI GGATG 1 cut(s) 186
BtsI GCAGTG 2 cut(s) 145, 318
BtsIMutI CAGTG 2 cut(s) 145, 318
Cac8I GCNNGC 1 cut(s) 246
Cfr13I GGNCC 2 cut(s) 70, 131
CviAII CATG 2 cut(s) 86, 275
DdeI CTNAG 3 cut(s) 300, 339, 368
DpnI GATC 3 cut(s) 144, 424, 497
DpnII GATC 3 cut(s) 142, 422, 495
Eam1104I CTCTTC 2 cut(s) 141, 391
EarI CTCTTC 2 cut(s) 141, 391
Eco130I CCWWGG 1 cut(s) 274
Eco47I GGWCC 1 cut(s) 70
Eco57I CTGAAG 2 cut(s) 86, 497
EcoO109I RGGNCCY 1 cut(s) 70
EcoT14I CCWWGG 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 274
FaeI CATG 2 cut(s) 89, 278
FaiI YATR 3 cut(s) 87, 276, 544
FalI AAGNNNNNCTT 2 cut(s) 361, 393
FatI CATG 2 cut(s) 85, 274
FblI GTMKAC 1 cut(s) 23
Fnu4HI GCNGC 2 cut(s) 197, 291
FokI GGATG 1 cut(s) 173
Fsp4HI GCNGC 2 cut(s) 197, 291
FspBI CTAG 1 cut(s) 359
GluI GCNGC 2 cut(s) 197, 291
HaeIII GGCC 2 cut(s) 132, 216
HapII CCGG 1 cut(s) 486
Hin1II CATG 2 cut(s) 89, 278
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HinfI GANTC 1 cut(s) 270
HpaII CCGG 1 cut(s) 486
HphI GGTGA 2 cut(s) 214, 421
Hpy166II GTNNAC 1 cut(s) 24
Hpy188I TCNGA 1 cut(s) 450
Hpy188III TCNNGA 1 cut(s) 140
Hpy8I GTNNAC 1 cut(s) 24
Hpy99I CGWCG 1 cut(s) 25
HpyAV CCTTC 3 cut(s) 110, 472, 526
HpyCH4III ACNGT 2 cut(s) 331, 454
HpyCH4V TGCA 3 cut(s) 150, 323, 404
HpyF10VI GCNNNNNNNGC 3 cut(s) 53, 290, 296
HpyF3I CTNAG 3 cut(s) 300, 339, 368
Hsp92II CATG 2 cut(s) 89, 278
Kzo9I GATC 3 cut(s) 142, 422, 495
LmnI GCTCC 3 cut(s) 281, 349, 397
LpnPI CCDG 7 cut(s) 90, 114, 153, 238, 258, 449, 499
Lsp1109I GCAGC 1 cut(s) 277
LweI GCATC 3 cut(s) 103, 195, 381
MaeI CTAG 1 cut(s) 359
MaeIII GTNAC 1 cut(s) 220
MalI GATC 3 cut(s) 144, 424, 497
MbiI CCGCTC 2 cut(s) 199, 506
MboI GATC 3 cut(s) 142, 422, 495
MboII GAAGA 4 cut(s) 19, 128, 378, 453
MflI RGATCY 1 cut(s) 422
MluCI AATT 3 cut(s) 113, 491, 539
MspI CCGG 1 cut(s) 486
MspR9I CCNGG 1 cut(s) 487
Mva1269I GAATGC 1 cut(s) 406
MwoI GCNNNNNNNGC 3 cut(s) 53, 290, 296
NciI CCSGG 1 cut(s) 487
NcoI CCATGG 1 cut(s) 274
NdeII GATC 3 cut(s) 142, 422, 495
NlaIII CATG 2 cut(s) 89, 278
NmeAIII GCCGAG 1 cut(s) 162
NmuCI GTSAC 1 cut(s) 220
PctI GAATGC 1 cut(s) 406
PfeI GAWTC 1 cut(s) 270
PkrI GCNGC 2 cut(s) 198, 292
PpuMI RGGWCCY 1 cut(s) 70
Psp5II RGGWCCY 1 cut(s) 70
PspPI GGNCC 2 cut(s) 70, 131
PspPPI RGGWCCY 1 cut(s) 70
PsuI RGATCY 1 cut(s) 422
SalI GTCGAC 1 cut(s) 22
SatI GCNGC 2 cut(s) 197, 291
Sau3AI GATC 3 cut(s) 142, 422, 495
Sau96I GGNCC 2 cut(s) 70, 131
ScrFI CCNGG 1 cut(s) 487
SfaNI GCATC 3 cut(s) 103, 195, 381
SfcI CTRYAG 1 cut(s) 426
SinI GGWCC 1 cut(s) 70
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
Sse9I AATT 3 cut(s) 113, 491, 539
SsiI CCGC 3 cut(s) 197, 433, 504
SspMI CTAG 1 cut(s) 359
StyD4I CCNGG 1 cut(s) 485
StyI CCWWGG 1 cut(s) 274
TaaI ACNGT 2 cut(s) 331, 454
TaqI TCGA 2 cut(s) 23, 211
TasI AATT 3 cut(s) 113, 491, 539
TauI GCSGC 1 cut(s) 199
TfiI GAWTC 1 cut(s) 270
TscAI CASTG 2 cut(s) 152, 325
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 1 cut(s) 290
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 1 cut(s) 197
TspGWI ACGGA 1 cut(s) 226
TspRI CASTG 2 cut(s) 152, 325
VpaK11BI GGWCC 1 cut(s) 70
XmiI GTMKAC 1 cut(s) 23
XspI CTAG 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.