Rorug01G0231800
ERF Family

Thioesterase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002384
Physical Location & Seq
Forward (+)
33331010 .. 33351431
20422 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0231800.1

Sequence Viewer

Length: 3627 bp
ATGGATGTTGTTAGCTCATCATCATCAGCTCAACAAGCTTCGTCTTCCAGTATTCCTCCTAGTCAATTCACATATCAAGTGTTCTTGAGTTTCAGAGGCAGAAACACTCGCAAGAGTTTCACCGATCATCTCTACACAGCCCTGACCAATGCAGGAATCTGCACTTTCCGAGACGACGATGAGCTCCCGGCCGGAGAAGCCATCAAACCAGAACTAGAGAGAGCCATCCAGCACTCGCGATGTTCTGTTATTGTGTTTTCAAAAGACTACGCGTCGTCCGGATGGTGCCTGGACGAGCTTGTGGCGATCCTTCGATGCAAGAAGAGGTCCGGTCAGTTCGTTTTACCGATCTACTACGACGTCAGCCCATCCCAGGTGAGGAAGCAGACGGGGAGTCTTGCAAAGGCGTTCATCACACACCAGAAGAATCAAACAGCGGAAAAGCTGATTCTGTGGAAGGAAGCACTTACAGAAGTTGCGGCTCTAGCAGGCATGGTGTTAAAGGATCAAGTTGACGGGCACGAGTCAAAATTTATTGAAAATATTGTTAAGGTGATTGAAGGCAAGTTATGTCGAGTCCCCTTATGTGTTGCCCCTTACCTGGTTGGAATTGATTCTCGAGTAAAAAGCATGAATTTATGGTTACAGGATGGATCGTCCAATATTGGTATATTAGGAATTTGTGGAATTGGTGGAATAGGAAAGACAACTATTGCACAAATTGTTTATAATTCAAATTTTGAGAGATTTGAAAGAAGCTGCTTTCTTGAAAATATCAGAGAGATTTTCGAACGGCCAAGTGGTTTTGTTCAAATACAGAAACAGCTTCTTTCGGACATTTTGAATGGGAGAAAAGTGAATATAAGCAGTCCTAGCGAGGGAATGAGTAAAATAAAAGATGCCTTAAGCTCTAAAAAAGTTCTTCTTGTTCTCGATGATGTGGATCACAAGGGCCTAGTTGATGCAATACTTGAGATGAAAGGCTGCTTTTGTTCTGGAAGTAAAATTATAATAACAACAAGGAATGCAGGATTGCTCAAGGGTCGTCGAGATATTAAGGTGCATAATGTTGAAACTTTGAACCATATTGAATCATCGGACCTCTTCAGTAGGCATGCTTTTGGACACAACTGCCCTATTGAAAGTTACGTGAAACTTTCAGACAGGGTAGTGCACCACTGCGAAGGACTTCCGTTAGCTCTTCAAACATTGGGTTCTTCTCTCTCAGGGCAGACTATAGATGTATGGGAAAGTGCATTAAAGAAATCCAAAACTATACCAAACAGTGTAGTAATGAGTAAACTGAGGACGAGCTTCAACTCTTTACAAGATGACCATGATCAGAGTTTATTCCTCCATATTGCATGTTTTTTTATCGGAAAGGACAAGGATATCATTGTCAAAATTCTAGATGCATGTGATTTCTACACAACCGTTGGCATTCAAAATCTCATTGATAGATATCTGGTTACAATTGATGAATGTAATAAGGTGAATATGCATCACATGATTTGTGACATGGGGAGAGAAATTGTTCGTCTGGAATCAAAGGATCCTGAGAAACGTAGTAGGCTATGGCGTCACAAGGACTCTCTTAATGTTCTAAGAGAAAAGACTGGTTCAAATGCAATTGAAGGCCTTGTCCTGAACGTGCCTGTGAACCCTGCACATGGTCCTTCAAGAAATTCAGATATGGTGTTCTTGGATACCAATGCATTTTCAATGATTAACAAACTCCGATTCCTTCATCTTGGTCAAGTACAACTCAATGGAAGTTATGAAAAGTTTCCTAAAGGAGTGAGATGGTTGTGTTGGCGCGAATGTCCTTTGAATTTAATACCAAGTGATTTTCCTATGGAGGTGATGGTTGTTATTGAAATGTCCTGTAGTAGATTGAGACAAGCGTGGAAGGGAGCAAAGTGTCTTCCTTCATTGAAGATCTTTGATCTCAGCCATTCCTCAAACCTTACTGAAACTGGTGACTTCTCATTGGTCCAGAATCTGGAGAGACTTACTCTTAAAGATTGTATCAGCTTAGTTGATGTCCATGAATCCATAGGAAAGCTAGAGAAACTTGTTTACTTGAATTTGAAAGATTGCATAAATTTCATGAAGCTTCCAGAGAACATTCATATGTTAAAATCACTTGAAACACTTATTATATCTGGTTGTTCAAATCTTAGTGAGTTTCCAATGGAGATTAGATACATGGAATCTCTTAAAGTGTTTCAAGCATTTGGAGTTCCAATAGATCAGCTGATCACTATCCCTGGGCAGGTAAATTCACGGCCAAGATTAAGTGTACGTGATTTTTGGGGCTCCTTTCCTGTCTCTCTTGTTGATTTAAATCTTGGAAACTGCAATCTTTCTGAGGATGCATTTCCTATTGATTTTGCTGCATTAGCTTCGTTGAAGAGTTTGGATCTTAGAGGAAATAATTTTGTTACCTTGCCAAGTTCAATCAGAAGCCTTTGTAACCTCGAGACTCTCGATCTTCAATCCTGTACACATCTCAGGTTAATTACAGGTCTACCACCAGTAAGAGATCTGCTGAGCACAGACGGTTGTGTGTCCTTGGAAAAAATAACATTTCAATCACTTCCGTGCATACCAAAGAGAGTATCCATATATGGTGACTCCAAACTAGTTGAGATTCAGCACTGGTACAAGTTAAAACCCATTGGAACGGTTGATGTAGAAATGATCAGGCTTTTGGGTTTGTCCAACTTGGAATCCATGGAACCCATTCTGATGCACACCCCAGATGCAAACTCCCCATCAGAAGGGGAGTCCATGGAACCCATTCGCATCCAGGGACTGCATGAATATGGTATCTTCAGCACATTCTTTCCGGGAAACGGAAATGAGGTTCCAGGACAGTTCAGCCATACAAGTAAAGGGTGTTCCTCAATTTCTTTTCCTGTGCCTATACTTCTTAACCACAAGATCCGAGGCTTCAACATATATTCTGTGTATGCACGGACGACAGATGTTACTACTTTATTGCCACCATTAATCATTGAAGTCAATAATAAGAGCTCGGGTCTGAAGTGGATCTATGTGCCTTCATGTTACGGCATTCCAAGTGATGGAAAAGACATGATATGGTTAAGCCATTGGAGTTTGGGGAACCAATTCAAAGGTGGCGATGTAGTCACTGTCTCAATATTTACGCCAGATAAGTGGCTTCAGGTAAAAGAGTTCGGTGTCCAAGTGGTGCATGAGGAAGCAGAGAAGATGAGTAAGCAACAGAACAGCAGCAATAGTAGAGATCCTAGTTATGGTGATCCCGATGCTTGTGGTGTTAATGAGGTGATTGGTAATAGTGGTGGAGTTTTGTCAGAGTTATATCAGGTCAGGCCGGGGACATACTTCCTCTGCGGTGGACCCTTTTTAGAAAATAATAGATACACATATAACAGATGGAAAAAGGATCGTTGCCTCAGTGGCATCATAGGAGAGTATGATGAAGAAACAGACAAAGCTGAAAGGAAACAGGGGGATAGGACACTAGCAGCAACAGAAGAAGCTGAAGCAGAAAGAGGCATCAATAAATTCTTTGGTTGCAAGAACCTTGTTATCCATGTGCTTATGTCGTTGTTTGAGATTTGTCAAAATATCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4HBT

Protein Analysis

1208

Amino Acids

135.1

Weight (kDa)

6.68

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 25 - 189 1.1e-50 TIR domain
TIR_2 PF13676 27 - 139 6e-16 TIR domain
NB-ARC PF00931 211 - 375 8.8e-19 NB-ARC domain
WHD_ROQ1 PF23282 445 - 515 2.5e-19 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 665 - 742 4.1e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 729, 1010
AatII GACGTC 1 cut(s) 363
Acc36I ACCTGC 1 cut(s) 2266
AccB1I GGYRCC 1 cut(s) 285
AccB7I CCANNNNNTGG 2 cut(s) 2002, 3089
AccI GTMKAC 1 cut(s) 2530
AccII CGCG 3 cut(s) 238, 272, 1818
AccIII TCCGGA 1 cut(s) 278
AciI CCGC 3 cut(s) 437, 479, 3381
AcoI YGGCCR 3 cut(s) 189, 794, 2288
AcuI CTGAAG 5 cut(s) 1090, 2821, 3068, 3173, 3552
AcyI GRCGYC 2 cut(s) 360, 1579
AfaI GTAC 4 cut(s) 1761, 2304, 2506, 2666
AflII CTTAAG 1 cut(s) 904
AflIII ACRYGT 1 cut(s) 270
AhdI GACNNNNNGTC 2 cut(s) 271, 393
AhlI ACTAGT 1 cut(s) 2644
AjnI CCWGG 6 cut(s) 288, 372, 600, 2269, 2811, 2872
AleI CACNNNNGTG 1 cut(s) 2602
AloI GAACNNNNNNTCC 2 cut(s) 2853, 2885
Alw21I GWGCWC 4 cut(s) 186, 1176, 2558, 3041
Alw26I GTCTC 6 cut(s) 165, 1891, 2002, 2335, 2477, 3166
Alw44I GTGCAC 1 cut(s) 1172
AlwNI CAGNNNCTG 2 cut(s) 2002, 2818
Ama87I CYCGRG 3 cut(s) 618, 2480, 3040
Aor13HI TCCGGA 1 cut(s) 278
AoxI GGCC 6 cut(s) 189, 794, 952, 1636, 2288, 3359
ApaLI GTGCAC 1 cut(s) 1172
ApeKI GCWGC 5 cut(s) 759, 984, 2396, 3258, 3515
AseI ATTAAT 1 cut(s) 3014
Asp700I GAANNNNTTC 7 cut(s) 613, 1188, 1533, 1785, 2745, 2802, 3134
AspLEI GCGC 1 cut(s) 1818
AspS9I GGNCC 6 cut(s) 327, 952, 1099, 1673, 1993, 3386
AsuC2I CCSGG 3 cut(s) 188, 2853, 3363
AsuHPI GGTGA 9 cut(s) 112, 388, 565, 1504, 1873, 1991, 2645, 3296, 3325
AsuII TTCGAA 1 cut(s) 789
AvaI CYCGRG 3 cut(s) 618, 2480, 3040
AvaII GGWCC 5 cut(s) 327, 1099, 1673, 1993, 3386
BaeGI GKGCMC 2 cut(s) 522, 1176
BamHI GGATCC 1 cut(s) 1552
BanI GGYRCC 1 cut(s) 285
BanII GRGCYC 3 cut(s) 186, 2321, 3041
BauI CACGAG 1 cut(s) 521
BbsI GAAGAC 2 cut(s) 36, 1916
Bbv12I GWGCWC 4 cut(s) 186, 1176, 2558, 3041
BbvI GCAGC 5 cut(s) 746, 971, 2383, 3270, 3527
BceAI ACGGC 3 cut(s) 809, 2303, 3091
BcgI CGANNNNNNTGC 2 cut(s) 2388, 2422
BciT130I CCWGG 6 cut(s) 290, 374, 602, 2271, 2813, 2874
BciVI GTATCC 2 cut(s) 1699, 2632
BclI TGATCA 3 cut(s) 1339, 2259, 2703
BcnI CCSGG 3 cut(s) 188, 2853, 3363
BcoDI GTCTC 6 cut(s) 165, 1891, 2002, 2335, 2477, 3166
BcuI ACTAGT 1 cut(s) 2644
BfmI CTRYAG 2 cut(s) 1236, 1885
BfrI CTTAAG 1 cut(s) 904
BfuAI ACCTGC 1 cut(s) 2266
BfuI GTATCC 2 cut(s) 1699, 2632
BglI GCCNNNNNGGC 1 cut(s) 3447
BglII AGATCT 2 cut(s) 1938, 2545
BisI GCNGC 6 cut(s) 480, 760, 985, 2397, 3259, 3516
BlpI GCTNAGC 1 cut(s) 2552
BlsI GCNGC 6 cut(s) 481, 761, 986, 2398, 3260, 3517
Bme1390I CCNGG 9 cut(s) 188, 290, 374, 602, 2271, 2813, 2853, 2874, 3363
Bme18I GGWCC 5 cut(s) 327, 1099, 1673, 1993, 3386
BmeRI GACNNNNNGTC 2 cut(s) 271, 393
BmeT110I CYCGRG 3 cut(s) 618, 2480, 3040
BmgT120I GGNCC 6 cut(s) 327, 952, 1099, 1673, 1993, 3386
BmiI GGNNCC 8 cut(s) 287, 1554, 2320, 2742, 2799, 2871, 3131, 3388
BmrFI CCNGG 9 cut(s) 188, 290, 374, 602, 2271, 2813, 2853, 2874, 3363
BpiI GAAGAC 2 cut(s) 36, 1916
BplI GAGNNNNNCTC 2 cut(s) 1999, 2031
BpmI CTGGAG 1 cut(s) 2024
Bpu1102I GCTNAGC 1 cut(s) 2552
Bpu14I TTCGAA 1 cut(s) 789
BpuEI CTTGAG 3 cut(s) 106, 992, 1022
BpuMI CCSGG 3 cut(s) 188, 2853, 3363
BsaAI YACGTR 2 cut(s) 1150, 2306
BsaBI GATNNNNATC 4 cut(s) 942, 1461, 2264, 2346
BsaHI GRCGYC 2 cut(s) 360, 1579
BsaJI CCNNGG 9 cut(s) 372, 2269, 2270, 2574, 2736, 2793, 2812, 2950, 3362
BsaWI WCCGGW 2 cut(s) 278, 329
Bse1I ACTGG 5 cut(s) 48, 1621, 1981, 2537, 2666
Bse8I GATNNNNATC 4 cut(s) 942, 1461, 2264, 2346
BseAI TCCGGA 1 cut(s) 278
BseBI CCWGG 6 cut(s) 290, 374, 602, 2271, 2813, 2874
BseDI CCNNGG 9 cut(s) 372, 2269, 2270, 2574, 2736, 2793, 2812, 2950, 3362
BseGI GGATG 7 cut(s) 10, 225, 287, 368, 655, 2380, 2808
BseJI GATNNNNATC 4 cut(s) 942, 1461, 2264, 2346
BseMII CTCAG 8 cut(s) 1239, 1295, 1548, 1963, 2361, 2527, 2543, 3457
BseNI ACTGG 5 cut(s) 48, 1621, 1981, 2537, 2666
BseSI GKGCMC 2 cut(s) 522, 1176
BseX3I CGGCCG 1 cut(s) 189
BseXI GCAGC 5 cut(s) 746, 971, 2383, 3270, 3527
BsgI GTGCAG 2 cut(s) 145, 1650
Bsh1236I CGCG 3 cut(s) 238, 272, 1818
Bsh1285I CGRYCG 1 cut(s) 192
BshFI GGCC 6 cut(s) 191, 796, 954, 1638, 2290, 3361
BshNI GGYRCC 1 cut(s) 285
BsiEI CGRYCG 1 cut(s) 192
BsiHKAI GWGCWC 4 cut(s) 186, 1176, 2558, 3041
BsiHKCI CYCGRG 3 cut(s) 618, 2480, 3040
BsiSI CCGG 6 cut(s) 188, 192, 279, 330, 2852, 3362
BslFI GGGAC 3 cut(s) 563, 2829, 3379
BsmAI GTCTC 6 cut(s) 165, 1891, 2002, 2335, 2477, 3166
BsmBI CGTCTC 1 cut(s) 165
BsmFI GGGAC 3 cut(s) 563, 2829, 3379
BsmI GAATGC 3 cut(s) 1030, 1440, 3078
BsnI GGCC 6 cut(s) 191, 796, 954, 1638, 2290, 3361
BsoBI CYCGRG 3 cut(s) 618, 2480, 3040
Bsp119I TTCGAA 1 cut(s) 789
Bsp1286I GDGCHC 6 cut(s) 186, 522, 1176, 2321, 2558, 3041
Bsp13I TCCGGA 1 cut(s) 278
Bsp1407I TGTACA 1 cut(s) 2504
Bsp1720I GCTNAGC 1 cut(s) 2552
Bsp19I CCATGG 2 cut(s) 2736, 2793
Bsp68I TCGCGA 1 cut(s) 238
BspACI CCGC 3 cut(s) 437, 479, 3381
BspANI GGCC 6 cut(s) 191, 796, 954, 1638, 2290, 3361
BspCNI CTCAG 8 cut(s) 1238, 1296, 1549, 1962, 2362, 2526, 2544, 3456
BspEI TCCGGA 1 cut(s) 278
BspFNI CGCG 3 cut(s) 238, 272, 1818
BspHI TCATGA 1 cut(s) 2109
BspLI GGNNCC 8 cut(s) 287, 1554, 2320, 2742, 2799, 2871, 3131, 3388
BspMI ACCTGC 1 cut(s) 2266
BspQI GCTCTTC 1 cut(s) 1206
BspT104I TTCGAA 1 cut(s) 789
BspT107I GGYRCC 1 cut(s) 285
BspTI CTTAAG 1 cut(s) 904
BsrGI TGTACA 1 cut(s) 2504
BsrI ACTGG 5 cut(s) 48, 1621, 1981, 2537, 2666
BssECI CCNNGG 9 cut(s) 372, 2269, 2270, 2574, 2736, 2793, 2812, 2950, 3362
BssNI GRCGYC 2 cut(s) 360, 1579
BssSI CACGAG 1 cut(s) 521
BssT1I CCWWGG 3 cut(s) 2574, 2736, 2793
Bst2BI CACGAG 1 cut(s) 521
Bst2UI CCWGG 6 cut(s) 290, 374, 602, 2271, 2813, 2874
Bst4CI ACNGT 6 cut(s) 1286, 1435, 2564, 2689, 2880, 3160
Bst6I CTCTTC 4 cut(s) 317, 1109, 1206, 2408
BstACI GRCGYC 2 cut(s) 360, 1579
BstAFI CTTAAG 1 cut(s) 904
BstAUI TGTACA 1 cut(s) 2504
BstBAI YACGTR 2 cut(s) 1150, 2306
BstBI TTCGAA 1 cut(s) 789
BstC8I GCNNGC 2 cut(s) 490, 1116
BstDSI CCRYGG 2 cut(s) 2736, 2793
BstF5I GGATG 7 cut(s) 10, 225, 287, 368, 655, 2380, 2808
BstFNI CGCG 3 cut(s) 238, 272, 1818
BstHHI GCGC 1 cut(s) 1818
BstMAI GTCTC 6 cut(s) 165, 1891, 2002, 2335, 2477, 3166
BstMCI CGRYCG 1 cut(s) 192
BstMWI GCNNNNNNNGC 6 cut(s) 35, 197, 485, 873, 2402, 3447
BstNI CCWGG 6 cut(s) 290, 374, 602, 2271, 2813, 2874
BstNSI RCATGY 3 cut(s) 1118, 1368, 1419
BstSCI CCNGG 9 cut(s) 186, 288, 372, 600, 2269, 2811, 2851, 2872, 3361
BstSFI CTRYAG 2 cut(s) 1236, 1885
BstSLI GKGCMC 2 cut(s) 522, 1176
BstUI CGCG 3 cut(s) 238, 272, 1818
BstV1I GCAGC 5 cut(s) 746, 971, 2383, 3270, 3527
BstV2I GAAGAC 2 cut(s) 36, 1916
BstX2I RGATCY 7 cut(s) 1552, 1938, 2422, 2545, 2946, 3054, 3271
BstXI CCANNNNNNTGG 1 cut(s) 3183
BstYI RGATCY 7 cut(s) 1552, 1938, 2422, 2545, 2946, 3054, 3271
BstZI CGGCCG 1 cut(s) 189
BsuI GTATCC 2 cut(s) 1699, 2632
BsuRI GGCC 6 cut(s) 191, 796, 954, 1638, 2290, 3361
BtgI CCRYGG 2 cut(s) 2736, 2793
BtgZI GCGATG 2 cut(s) 253, 3162
BtsCI GGATG 7 cut(s) 10, 225, 287, 368, 655, 2380, 2808
BtsI GCAGTG 1 cut(s) 1177
BtsIMutI CAGTG 5 cut(s) 1177, 1291, 2659, 3156, 3451
BtuMI TCGCGA 1 cut(s) 238
BveI ACCTGC 1 cut(s) 2266
Cac8I GCNNGC 2 cut(s) 490, 1116
CaiI CAGNNNCTG 2 cut(s) 2002, 2818
CciI TCATGA 1 cut(s) 2109
CfoI GCGC 1 cut(s) 1818
Cfr13I GGNCC 6 cut(s) 327, 952, 1099, 1673, 1993, 3386
CseI GACGC 2 cut(s) 261, 1568
CsiI ACCWGGT 1 cut(s) 600
Csp6I GTAC 4 cut(s) 1760, 2303, 2505, 2665
CviQI GTAC 4 cut(s) 1760, 2303, 2505, 2665
DraI TTTAAA 1 cut(s) 2346
DriI GACNNNNNGTC 2 cut(s) 271, 393
EaeI YGGCCR 3 cut(s) 189, 794, 2288
EagI CGGCCG 1 cut(s) 189
Eam1104I CTCTTC 4 cut(s) 317, 1109, 1206, 2408
Eam1105I GACNNNNNGTC 2 cut(s) 271, 393
EarI CTCTTC 4 cut(s) 317, 1109, 1206, 2408
Ecl136II GAGCTC 2 cut(s) 184, 3039
EclXI CGGCCG 1 cut(s) 189
Eco130I CCWWGG 3 cut(s) 2574, 2736, 2793
Eco147I AGGCCT 1 cut(s) 1638
Eco24I GRGCYC 3 cut(s) 186, 2321, 3041
Eco32I GATATC 2 cut(s) 1393, 1463
Eco47I GGWCC 5 cut(s) 327, 1099, 1673, 1993, 3386
Eco52I CGGCCG 1 cut(s) 189
Eco53kI GAGCTC 2 cut(s) 184, 3039
Eco57I CTGAAG 5 cut(s) 1090, 2821, 3068, 3173, 3552
Eco88I CYCGRG 3 cut(s) 618, 2480, 3040
EcoICRI GAGCTC 2 cut(s) 184, 3039
EcoO109I RGGNCCY 1 cut(s) 952
EcoRII CCWGG 6 cut(s) 288, 372, 600, 2269, 2811, 2872
EcoRV GATATC 2 cut(s) 1393, 1463
EcoT14I CCWWGG 3 cut(s) 2574, 2736, 2793
EcoT22I ATGCAT 4 cut(s) 1417, 1503, 1717, 2380
EcoT38I GRGCYC 3 cut(s) 186, 2321, 3041
ErhI CCWWGG 3 cut(s) 2574, 2736, 2793
Esp3I CGTCTC 1 cut(s) 165
FalI AAGNNNNNCTT 2 cut(s) 909, 941
FaqI GGGAC 3 cut(s) 563, 2829, 3379
FauNDI CATATG 1 cut(s) 2133
FbaI TGATCA 3 cut(s) 1339, 2259, 2703
FblI GTMKAC 1 cut(s) 2530
Fnu4HI GCNGC 6 cut(s) 480, 760, 985, 2397, 3259, 3516
FokI GGATG 7 cut(s) 17, 212, 294, 355, 662, 2387, 2795
FriOI GRGCYC 3 cut(s) 186, 2321, 3041
Fsp4HI GCNGC 6 cut(s) 480, 760, 985, 2397, 3259, 3516
GlaI GCGC 1 cut(s) 1817
GluI GCNGC 6 cut(s) 480, 760, 985, 2397, 3259, 3516
GsuI CTGGAG 1 cut(s) 2024
HaeIII GGCC 6 cut(s) 191, 796, 954, 1638, 2290, 3361
HapII CCGG 6 cut(s) 188, 192, 279, 330, 2852, 3362
HgaI GACGC 2 cut(s) 261, 1568
HhaI GCGC 1 cut(s) 1818
Hin1I GRCGYC 2 cut(s) 360, 1579
Hin6I GCGC 1 cut(s) 1816
HinP1I GCGC 1 cut(s) 1816
HincII GTYRAC 1 cut(s) 514
HindII GTYRAC 1 cut(s) 514
HindIII AAGCTT 2 cut(s) 36, 2114
HpaII CCGG 6 cut(s) 188, 192, 279, 330, 2852, 3362
HphI GGTGA 9 cut(s) 112, 388, 565, 1504, 1873, 1991, 2645, 3296, 3325
Hpy166II GTNNAC 9 cut(s) 514, 1174, 1301, 1660, 2080, 2303, 2507, 2531, 3386
Hpy8I GTNNAC 9 cut(s) 514, 1174, 1301, 1660, 2080, 2303, 2507, 2531, 3386
Hpy99I CGWCG 4 cut(s) 179, 277, 362, 1050
HpyCH4III ACNGT 6 cut(s) 1286, 1435, 2564, 2689, 2880, 3160
HpyCH4IV ACGT 5 cut(s) 360, 1149, 1564, 1650, 2305
HpyF10VI GCNNNNNNNGC 6 cut(s) 35, 197, 485, 873, 2402, 3447
HpySE526I ACGT 5 cut(s) 360, 1149, 1564, 1650, 2305
Hsp92I GRCGYC 2 cut(s) 360, 1579
HspAI GCGC 1 cut(s) 1816
Kpn2I TCCGGA 1 cut(s) 278
Ksp22I TGATCA 3 cut(s) 1339, 2259, 2703
LguI GCTCTTC 1 cut(s) 1206
LmnI GCTCC 3 cut(s) 189, 1913, 2324
Lsp1109I GCAGC 5 cut(s) 746, 971, 2383, 3270, 3527
MabI ACCWGGT 1 cut(s) 600
MaeII ACGT 5 cut(s) 360, 1149, 1564, 1650, 2305
MfeI CAATTG 2 cut(s) 1473, 1629
MflI RGATCY 7 cut(s) 1552, 1938, 2422, 2545, 2946, 3054, 3271
MhlI GDGCHC 6 cut(s) 186, 522, 1176, 2321, 2558, 3041
MluI ACGCGT 1 cut(s) 270
MlyI GAGTC 7 cut(s) 403, 533, 585, 1583, 2479, 2630, 2798
MmeI TCCRAC 2 cut(s) 586, 2748
Mph1103I ATGCAT 4 cut(s) 1417, 1503, 1717, 2380
MroI TCCGGA 1 cut(s) 278
MroXI GAANNNNTTC 7 cut(s) 613, 1188, 1533, 1785, 2745, 2802, 3134
MslI CAYNNNNRTG 4 cut(s) 2132, 2602, 2750, 2826
MspA1I CMGCKG 2 cut(s) 437, 2257
MspCI CTTAAG 1 cut(s) 904
MspI CCGG 6 cut(s) 188, 192, 279, 330, 2852, 3362
MspR9I CCNGG 9 cut(s) 188, 290, 374, 602, 2271, 2813, 2853, 2874, 3363
MunI CAATTG 2 cut(s) 1473, 1629
Mva1269I GAATGC 3 cut(s) 1030, 1440, 3078
MvaI CCWGG 6 cut(s) 290, 374, 602, 2271, 2813, 2874
MvnI CGCG 3 cut(s) 238, 272, 1818
MwoI GCNNNNNNNGC 6 cut(s) 35, 197, 485, 873, 2402, 3447
NciI CCSGG 3 cut(s) 188, 2853, 3363
NcoI CCATGG 2 cut(s) 2736, 2793
NdeI CATATG 1 cut(s) 2133
NlaIV GGNNCC 8 cut(s) 287, 1554, 2320, 2742, 2799, 2871, 3131, 3388
NmuCI GTSAC 5 cut(s) 1514, 1580, 1979, 2633, 3154
NruI TCGCGA 1 cut(s) 238
NsiI ATGCAT 4 cut(s) 1417, 1503, 1717, 2380
NspI RCATGY 3 cut(s) 1118, 1368, 1419
NspV TTCGAA 1 cut(s) 789
OliI CACNNNNGTG 1 cut(s) 2602
PaeI GCATGC 1 cut(s) 1118
PaeR7I CTCGAG 2 cut(s) 618, 2480
PagI TCATGA 1 cut(s) 2109
PasI CCCWGGG 1 cut(s) 2270
PceI AGGCCT 1 cut(s) 1638
PciSI GCTCTTC 1 cut(s) 1206
PcsI WCGNNNNNNNCGW 1 cut(s) 2487
PctI GAATGC 3 cut(s) 1030, 1440, 3078
PdmI GAANNNNTTC 7 cut(s) 613, 1188, 1533, 1785, 2745, 2802, 3134
PflMI CCANNNNNTGG 2 cut(s) 2002, 3089
PfoI TCCNGGA 2 cut(s) 2851, 2872
PkrI GCNGC 6 cut(s) 481, 761, 986, 2398, 3260, 3517
PleI GAGTC 7 cut(s) 402, 532, 584, 1583, 2479, 2630, 2797
PpsI GAGTC 7 cut(s) 402, 532, 584, 1583, 2479, 2630, 2797
Ppu21I YACGTR 2 cut(s) 1150, 2306
PshBI ATTAAT 1 cut(s) 3014
PsiI TTATAA 2 cut(s) 729, 1010
Psp124BI GAGCTC 2 cut(s) 186, 3041
Psp6I CCWGG 6 cut(s) 288, 372, 600, 2269, 2811, 2872
PspGI CCWGG 6 cut(s) 288, 372, 600, 2269, 2811, 2872
PspN4I GGNNCC 8 cut(s) 287, 1554, 2320, 2742, 2799, 2871, 3131, 3388
PspPI GGNCC 6 cut(s) 327, 952, 1099, 1673, 1993, 3386
PsrI GAACNNNNNNTAC 2 cut(s) 2887, 2919
PstNI CAGNNNCTG 2 cut(s) 2002, 2818
PsuI RGATCY 7 cut(s) 1552, 1938, 2422, 2545, 2946, 3054, 3271
PvuII CAGCTG 1 cut(s) 2257
RruI TCGCGA 1 cut(s) 238
RsaI GTAC 4 cut(s) 1761, 2304, 2506, 2666
RsaNI GTAC 4 cut(s) 1760, 2303, 2505, 2665
RseI CAYNNNNRTG 4 cut(s) 2132, 2602, 2750, 2826
SacI GAGCTC 2 cut(s) 186, 3041
SapI GCTCTTC 1 cut(s) 1206
SatI GCNGC 6 cut(s) 480, 760, 985, 2397, 3259, 3516
Sau96I GGNCC 6 cut(s) 327, 952, 1099, 1673, 1993, 3386
SchI GAGTC 7 cut(s) 403, 533, 585, 1583, 2479, 2630, 2798
ScrFI CCNGG 9 cut(s) 188, 290, 374, 602, 2271, 2813, 2853, 2874, 3363
SduI GDGCHC 6 cut(s) 186, 522, 1176, 2321, 2558, 3041
SexAI ACCWGGT 1 cut(s) 600
SfcI CTRYAG 2 cut(s) 1236, 1885
Sfr274I CTCGAG 2 cut(s) 618, 2480
SfuI TTCGAA 1 cut(s) 789
SinI GGWCC 5 cut(s) 327, 1099, 1673, 1993, 3386
SlaI CTCGAG 2 cut(s) 618, 2480
SmiI ATTTAAAT 1 cut(s) 2346
SmiMI CAYNNNNRTG 4 cut(s) 2132, 2602, 2750, 2826
SmlI CTYRAG 6 cut(s) 85, 618, 904, 971, 1037, 2480
SmoI CTYRAG 6 cut(s) 85, 618, 904, 971, 1037, 2480
SpeI ACTAGT 1 cut(s) 2644
SphI GCATGC 1 cut(s) 1118
SseBI AGGCCT 1 cut(s) 1638
SsiI CCGC 3 cut(s) 437, 479, 3381
SspI AATATT 3 cut(s) 544, 664, 3168
SstI GAGCTC 2 cut(s) 186, 3041
StuI AGGCCT 1 cut(s) 1638
StyD4I CCNGG 9 cut(s) 186, 288, 372, 600, 2269, 2811, 2851, 2872, 3361
StyI CCWWGG 3 cut(s) 2574, 2736, 2793
SwaI ATTTAAAT 1 cut(s) 2346
TaaI ACNGT 6 cut(s) 1286, 1435, 2564, 2689, 2880, 3160
TaiI ACGT 5 cut(s) 363, 1152, 1567, 1653, 2308
TaqI TCGA 8 cut(s) 313, 574, 619, 789, 933, 1048, 2481, 2490
TatI WGTACW 2 cut(s) 1759, 2504
TauI GCSGC 1 cut(s) 482
TscAI CASTG 5 cut(s) 1184, 1291, 2666, 3163, 3451
TseFI GTSAC 5 cut(s) 1514, 1580, 1979, 2633, 3154
TseI GCWGC 5 cut(s) 759, 984, 2396, 3258, 3515
Tsp45I GTSAC 5 cut(s) 1514, 1580, 1979, 2633, 3154
TspGWI ACGGA 4 cut(s) 1182, 2592, 2874, 2995
TspRI CASTG 5 cut(s) 1184, 1291, 2666, 3163, 3451
Van91I CCANNNNNTGG 2 cut(s) 2002, 3089
Vha464I CTTAAG 1 cut(s) 904
VneI GTGCAC 1 cut(s) 1172
VpaK11BI GGWCC 5 cut(s) 327, 1099, 1673, 1993, 3386
VspI ATTAAT 1 cut(s) 3014
XbaI TCTAGA 1 cut(s) 1408
XceI RCATGY 3 cut(s) 1118, 1368, 1419
XcmI CCANNNNNNNNNTGG 1 cut(s) 3140
XhoI CTCGAG 2 cut(s) 618, 2480
XmiI GTMKAC 1 cut(s) 2530
XmnI GAANNNNTTC 7 cut(s) 613, 1188, 1533, 1785, 2745, 2802, 3134
ZraI GACGTC 1 cut(s) 361
Zsp2I ATGCAT 4 cut(s) 1417, 1503, 1717, 2380
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.