RLG00000028313
ERF Family

Thioesterase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002384
Physical Location & Seq
Forward (+)
21325762 .. 21327310
1549 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028313

Sequence Viewer

Length: 543 bp
ATGGCAAAGCCCCCAATTGCTCTCTCAGAGAACTTTGCTTCCAATTCCAGAGCAACAATTTCCAAAGACATTCCTGAAGAACACTTGAACCAACTGCTGAGGTTTCTTGAAAGAGTGGGGATCTCTGGCAATGTCCCAGATTATTGCAATACCGAAGACTTCTATTCCGACCTCATTGGTGGCATCCTCAAGCCCCTGACAGTCGTACGAGGCCGCATCACTTGCTTGGTCTCTGTAAAACCTGCTGTTACTAATAAATTTGGAGGATTTCATGGAGGGGCTATTGCTGCTGTTGCTGAGGCTGTGTCATTTGCTACTGTAAGAACGGTCGTGGCTGAGGATAAGGAGCTTTTTCTAGGGGAACTAAGCATCTCTTACCTCTCTTCTGCACCAAAGAATGCAGAGGTGATAATTGATGGATCTGTAGTGCGGAGTGGGAGAAACTTAACTGTGGTAGCACTGGAGTTCAAGCTGAAGAAAACTGGGAACTTAGTGTACACTGCTCGTGCTACCTTATATCACATGCCCGTTTCGAAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4HBT

Protein Analysis

181

Amino Acids

19.4

Weight (kDa)

8.89

Isoelectric Point (pI)

25.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HBT_3 PF13622 86 - 171 2.6e-06 Acyl-CoA thioesterase N-terminal domain
4HBT PF03061 88 - 161 1.4e-08 Thioesterase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 250
AciI CCGC 2 cut(s) 214, 430
AclWI GGATC 2 cut(s) 128, 427
AcsI RAATTY 1 cut(s) 257
AcuI CTGAAG 2 cut(s) 96, 494
AfaI GTAC 2 cut(s) 207, 497
AgsI TTSAA 3 cut(s) 88, 110, 469
AloI GAACNNNNNNTCC 2 cut(s) 23, 55
AluBI AGCT 2 cut(s) 349, 472
AluI AGCT 2 cut(s) 349, 472
Alw26I GTCTC 1 cut(s) 235
AlwI GGATC 2 cut(s) 128, 427
AoxI GGCC 1 cut(s) 211
ApeKI GCWGC 1 cut(s) 287
ApoI RAATTY 1 cut(s) 257
AsuHPI GGTGA 1 cut(s) 418
AsuII TTCGAA 1 cut(s) 533
BauI CACGAG 1 cut(s) 504
BbsI GAAGAC 1 cut(s) 162
BbvCI CCTCAGC 3 cut(s) 98, 297, 336
BbvI GCAGC 1 cut(s) 274
BccI CCATC 1 cut(s) 410
BcoDI GTCTC 1 cut(s) 235
BfaI CTAG 1 cut(s) 356
BfmI CTRYAG 1 cut(s) 423
BfuAI ACCTGC 1 cut(s) 250
BisI GCNGC 2 cut(s) 214, 288
BlsI GCNGC 2 cut(s) 215, 289
BmrI ACTGGG 1 cut(s) 492
BmsI GCATC 3 cut(s) 192, 225, 378
BmuI ACTGGG 1 cut(s) 492
BpiI GAAGAC 1 cut(s) 162
BpmI CTGGAG 1 cut(s) 482
Bpu10I CCTNAGC 3 cut(s) 98, 297, 336
Bpu14I TTCGAA 1 cut(s) 533
BpuEI CTTGAG 1 cut(s) 173
BsaI GGTCTC 1 cut(s) 235
Bse1I ACTGG 2 cut(s) 465, 487
Bse3DI GCAATG 1 cut(s) 136
BseGI GGATG 1 cut(s) 183
BseMI GCAATG 1 cut(s) 136
BseMII CTCAG 4 cut(s) 39, 89, 288, 327
BseNI ACTGG 2 cut(s) 465, 487
BseXI GCAGC 1 cut(s) 274
BsgI GTGCAG 1 cut(s) 372
Bsh1285I CGRYCG 1 cut(s) 330
BshFI GGCC 1 cut(s) 213
BsiEI CGRYCG 1 cut(s) 330
BsiWI CGTACG 1 cut(s) 205
BslFI GGGAC 1 cut(s) 119
BsmAI GTCTC 1 cut(s) 235
BsmFI GGGAC 1 cut(s) 119
BsmI GAATGC 1 cut(s) 403
BsnI GGCC 1 cut(s) 213
Bso31I GGTCTC 1 cut(s) 235
Bsp119I TTCGAA 1 cut(s) 533
Bsp1407I TGTACA 1 cut(s) 495
Bsp143I GATC 2 cut(s) 120, 419
BspACI CCGC 2 cut(s) 214, 430
BspANI GGCC 1 cut(s) 213
BspCNI CTCAG 4 cut(s) 38, 90, 289, 328
BspMI ACCTGC 1 cut(s) 250
BspPI GGATC 2 cut(s) 128, 427
BspT104I TTCGAA 1 cut(s) 533
BspTNI GGTCTC 1 cut(s) 235
BsrDI GCAATG 1 cut(s) 136
BsrGI TGTACA 1 cut(s) 495
BsrI ACTGG 2 cut(s) 465, 487
BssMI GATC 2 cut(s) 120, 419
BssSI CACGAG 1 cut(s) 504
Bst2BI CACGAG 1 cut(s) 504
Bst4CI ACNGT 4 cut(s) 202, 319, 328, 451
Bst6I CTCTTC 1 cut(s) 388
BstAPI GCANNNNNTGC 1 cut(s) 222
BstAUI TGTACA 1 cut(s) 495
BstBI TTCGAA 1 cut(s) 533
BstDEI CTNAG 6 cut(s) 25, 98, 297, 336, 365, 490
BstF5I GGATG 1 cut(s) 183
BstKTI GATC 2 cut(s) 123, 422
BstMAI GTCTC 1 cut(s) 235
BstMBI GATC 2 cut(s) 120, 419
BstMCI CGRYCG 1 cut(s) 330
BstMWI GCNNNNNNNGC 3 cut(s) 222, 287, 293
BstNSI RCATGY 1 cut(s) 526
BstSFI CTRYAG 1 cut(s) 423
BstV1I GCAGC 1 cut(s) 274
BstV2I GAAGAC 1 cut(s) 162
BstX2I RGATCY 2 cut(s) 120, 419
BstYI RGATCY 2 cut(s) 120, 419
BsuRI GGCC 1 cut(s) 213
BtsCI GGATG 1 cut(s) 183
BtsI GCAGTG 1 cut(s) 498
BtsIMutI CAGTG 2 cut(s) 458, 498
BveI ACCTGC 1 cut(s) 250
Csp6I GTAC 2 cut(s) 206, 496
CviAII CATG 2 cut(s) 272, 523
CviJI RGCY 8 cut(s) 10, 193, 213, 281, 302, 335, 349, 472
CviKI_1 RGCY 8 cut(s) 10, 193, 213, 281, 302, 335, 349, 472
CviQI GTAC 2 cut(s) 206, 496
DdeI CTNAG 6 cut(s) 25, 98, 297, 336, 365, 490
DpnI GATC 2 cut(s) 122, 421
DpnII GATC 2 cut(s) 120, 419
Eam1104I CTCTTC 1 cut(s) 388
EarI CTCTTC 1 cut(s) 388
Eco31I GGTCTC 1 cut(s) 235
Eco57I CTGAAG 2 cut(s) 96, 494
FaeI CATG 2 cut(s) 275, 526
FaiI YATR 3 cut(s) 273, 517, 524
FalI AAGNNNNNCTT 2 cut(s) 358, 390
FaqI GGGAC 1 cut(s) 119
FatI CATG 2 cut(s) 271, 522
Fnu4HI GCNGC 2 cut(s) 214, 288
FokI GGATG 1 cut(s) 170
Fsp4HI GCNGC 2 cut(s) 214, 288
FspBI CTAG 1 cut(s) 356
GluI GCNGC 2 cut(s) 214, 288
GsuI CTGGAG 1 cut(s) 482
HaeIII GGCC 1 cut(s) 213
Hin1II CATG 2 cut(s) 275, 526
HphI GGTGA 1 cut(s) 418
Hpy166II GTNNAC 2 cut(s) 496, 498
Hpy188I TCNGA 2 cut(s) 28, 169
Hpy188III TCNNGA 3 cut(s) 48, 74, 107
Hpy8I GTNNAC 2 cut(s) 496, 498
HpyCH4III ACNGT 4 cut(s) 202, 319, 328, 451
HpyCH4V TGCA 3 cut(s) 147, 389, 401
HpyF10VI GCNNNNNNNGC 3 cut(s) 222, 287, 293
HpyF3I CTNAG 6 cut(s) 25, 98, 297, 336, 365, 490
Hsp92II CATG 2 cut(s) 275, 526
Kzo9I GATC 2 cut(s) 120, 419
LmnI GCTCC 1 cut(s) 346
LpnPI CCDG 8 cut(s) 61, 87, 111, 150, 209, 255, 446, 468
Lsp1109I GCAGC 1 cut(s) 274
LweI GCATC 3 cut(s) 192, 225, 378
MaeI CTAG 1 cut(s) 356
MaeIII GTNAC 1 cut(s) 247
MalI GATC 2 cut(s) 122, 421
MboI GATC 2 cut(s) 120, 419
MboII GAAGA 4 cut(s) 89, 167, 375, 487
MfeI CAATTG 1 cut(s) 15
MflI RGATCY 2 cut(s) 120, 419
MluCI AATT 6 cut(s) 15, 43, 57, 257, 411, 536
MmeI TCCRAC 1 cut(s) 192
MseI TTAA 1 cut(s) 446
MunI CAATTG 1 cut(s) 15
Mva1269I GAATGC 1 cut(s) 403
MwoI GCNNNNNNNGC 3 cut(s) 222, 287, 293
NdeII GATC 2 cut(s) 120, 419
NlaIII CATG 2 cut(s) 275, 526
NspI RCATGY 1 cut(s) 526
NspV TTCGAA 1 cut(s) 533
PctI GAATGC 1 cut(s) 403
Pfl23II CGTACG 1 cut(s) 205
PkrI GCNGC 2 cut(s) 215, 289
PspLI CGTACG 1 cut(s) 205
PsrI GAACNNNNNNTAC 2 cut(s) 479, 511
PsuI RGATCY 2 cut(s) 120, 419
RsaI GTAC 2 cut(s) 207, 497
RsaNI GTAC 2 cut(s) 206, 496
SaqAI TTAA 1 cut(s) 446
SatI GCNGC 2 cut(s) 214, 288
Sau3AI GATC 2 cut(s) 120, 419
SetI ASST 8 cut(s) 104, 174, 244, 351, 381, 408, 474, 515
SfaNI GCATC 3 cut(s) 192, 225, 378
SfcI CTRYAG 1 cut(s) 423
SfuI TTCGAA 1 cut(s) 533
SmlI CTYRAG 1 cut(s) 188
SmoI CTYRAG 1 cut(s) 188
Sse9I AATT 6 cut(s) 15, 43, 57, 257, 411, 536
SsiI CCGC 2 cut(s) 214, 430
SspMI CTAG 1 cut(s) 356
TaaI ACNGT 4 cut(s) 202, 319, 328, 451
TaqI TCGA 1 cut(s) 533
TasI AATT 6 cut(s) 15, 43, 57, 257, 411, 536
TatI WGTACW 1 cut(s) 495
TauI GCSGC 1 cut(s) 216
Tru1I TTAA 1 cut(s) 446
Tru9I TTAA 1 cut(s) 446
TscAI CASTG 2 cut(s) 465, 505
TseI GCWGC 1 cut(s) 287
TspDTI ATGAA 1 cut(s) 260
TspRI CASTG 2 cut(s) 465, 505
XapI RAATTY 1 cut(s) 257
XceI RCATGY 1 cut(s) 526
XspI CTAG 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.