Rroxscaffold_4G00302290
ERF Family

Thioesterase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002384
Physical Location & Seq
Forward (+)
22691812 .. 22694130
2319 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00302290.1

Sequence Viewer

Length: 573 bp
ATGAGGAAAATGGCAAAACCCTGTGGTACTCTGGCAGAGGGGTTTGCTTGCACAGCCTCAATTTCAGATGACGTCCCTGAAAAACAGGTCAAGGATACGCTGCATTTTCTCAAACAAGTGGGTATCTCCGGCCCTGTTCCAGATCACTGCGAGACCAAAGACTTCTACTCCTACCTCGTTCGTGGCATCCTTAAGCCCCTCAACATTCAACGAGGCCGTGTCACCTGCCTTGTCTCCGTTAAACCTGCTTTTATTGTAAAGCCTTCCATGCTTCTTCTCTCTAACTCTTTTGGTGGATTCCATGGAGGAGCTATAGCTGCTGTTGCTGAGGCTGTGTCAGTAGCTACTGCAAGAACGGTTGTGGCTGAGGATAAGGAGCTTTTTCTTGGGGAACTAAGCATCTCTTATCTCTCTTCTGCTCCAATGAATGCAGAGGTGATAGTTGATGGATCTGTAGTTCGGAGTGGAAGAAATCTTACTGTAATAGCACAGGAGTTCAAGCTCAAGAAAACTGGGAACTTGATCTACATTGCTCGTGCTACCTTCTATCACATGCCTGTTTCGAAATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4HBT

Protein Analysis

190

Amino Acids

20.49

Weight (kDa)

9.19

Isoelectric Point (pI)

28.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HBT PF03061 98 - 171 1.8e-09 Thioesterase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 233
AatII GACGTC 1 cut(s) 75
Acc36I ACCTGC 2 cut(s) 233, 253
AclWI GGATC 1 cut(s) 457
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 1 cut(s) 28
AflII CTTAAG 1 cut(s) 191
AgsI TTSAA 2 cut(s) 209, 499
AloI GAACNNNNNNTCC 2 cut(s) 441, 473
AluBI AGCT 5 cut(s) 311, 317, 344, 379, 502
AluI AGCT 5 cut(s) 311, 317, 344, 379, 502
Alw26I GTCTC 2 cut(s) 146, 238
AlwI GGATC 1 cut(s) 457
AoxI GGCC 2 cut(s) 130, 214
ApeKI GCWGC 2 cut(s) 100, 317
AspS9I GGNCC 1 cut(s) 131
AsuHPI GGTGA 2 cut(s) 214, 448
AsuII TTCGAA 1 cut(s) 563
BarI GAAGNNNNNNTAC 2 cut(s) 460, 492
BauI CACGAG 1 cut(s) 534
BbvCI CCTCAGC 2 cut(s) 327, 366
BbvI GCAGC 2 cut(s) 87, 304
BccI CCATC 1 cut(s) 440
BceAI ACGGC 1 cut(s) 201
BciVI GTATCC 1 cut(s) 88
BcoDI GTCTC 2 cut(s) 146, 238
BfmI CTRYAG 2 cut(s) 312, 453
BfrI CTTAAG 1 cut(s) 191
BfuAI ACCTGC 2 cut(s) 233, 253
BfuI GTATCC 1 cut(s) 88
BisI GCNGC 2 cut(s) 101, 318
BlsI GCNGC 2 cut(s) 102, 319
BmgT120I GGNCC 1 cut(s) 131
BmrI ACTGGG 1 cut(s) 522
BmsI GCATC 2 cut(s) 195, 408
BmuI ACTGGG 1 cut(s) 522
Bpu10I CCTNAGC 2 cut(s) 327, 366
Bpu14I TTCGAA 1 cut(s) 563
BpuEI CTTGAG 1 cut(s) 488
BsaHI GRCGYC 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 146
BsaJI CCNNGG 1 cut(s) 301
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bse1I ACTGG 1 cut(s) 517
Bse3DI GCAATG 1 cut(s) 528
BseDI CCNNGG 1 cut(s) 301
BseGI GGATG 1 cut(s) 186
BseMI GCAATG 1 cut(s) 528
BseMII CTCAG 2 cut(s) 318, 357
BseNI ACTGG 1 cut(s) 517
BseRI GAGGAG 1 cut(s) 321
BseXI GCAGC 2 cut(s) 87, 304
BshFI GGCC 2 cut(s) 132, 216
BsiSI CCGG 1 cut(s) 129
BslFI GGGAC 1 cut(s) 59
BsmAI GTCTC 2 cut(s) 146, 238
BsmFI GGGAC 1 cut(s) 59
BsmI GAATGC 1 cut(s) 433
BsnI GGCC 2 cut(s) 132, 216
Bso31I GGTCTC 1 cut(s) 146
Bsp119I TTCGAA 1 cut(s) 563
Bsp143I GATC 3 cut(s) 142, 449, 522
Bsp19I CCATGG 1 cut(s) 301
BspANI GGCC 2 cut(s) 132, 216
BspCNI CTCAG 2 cut(s) 319, 358
BspMI ACCTGC 2 cut(s) 233, 253
BspPI GGATC 1 cut(s) 457
BspT104I TTCGAA 1 cut(s) 563
BspTI CTTAAG 1 cut(s) 191
BspTNI GGTCTC 1 cut(s) 146
BsrDI GCAATG 1 cut(s) 528
BsrI ACTGG 1 cut(s) 517
BssECI CCNNGG 1 cut(s) 301
BssMI GATC 3 cut(s) 142, 449, 522
BssNI GRCGYC 1 cut(s) 72
BssSI CACGAG 1 cut(s) 534
BssT1I CCWWGG 1 cut(s) 301
Bst2BI CACGAG 1 cut(s) 534
Bst4CI ACNGT 2 cut(s) 358, 481
Bst6I CTCTTC 1 cut(s) 418
BstACI GRCGYC 1 cut(s) 72
BstAFI CTTAAG 1 cut(s) 191
BstBI TTCGAA 1 cut(s) 563
BstC8I GCNNGC 1 cut(s) 49
BstDEI CTNAG 3 cut(s) 327, 366, 395
BstDSI CCRYGG 1 cut(s) 301
BstF5I GGATG 1 cut(s) 186
BstKTI GATC 3 cut(s) 145, 452, 525
BstMAI GTCTC 2 cut(s) 146, 238
BstMBI GATC 3 cut(s) 142, 449, 522
BstMWI GCNNNNNNNGC 4 cut(s) 53, 268, 317, 323
BstNSI RCATGY 1 cut(s) 556
BstSFI CTRYAG 2 cut(s) 312, 453
BstV1I GCAGC 2 cut(s) 87, 304
BstX2I RGATCY 1 cut(s) 449
BstYI RGATCY 1 cut(s) 449
BsuI GTATCC 1 cut(s) 88
BsuRI GGCC 2 cut(s) 132, 216
BtgI CCRYGG 1 cut(s) 301
BtsCI GGATG 1 cut(s) 186
BtsI GCAGTG 1 cut(s) 145
BtsIMutI CAGTG 1 cut(s) 145
BveI ACCTGC 2 cut(s) 233, 253
Cac8I GCNNGC 1 cut(s) 49
Cfr13I GGNCC 1 cut(s) 131
Csp6I GTAC 1 cut(s) 27
CviAII CATG 3 cut(s) 268, 302, 553
CviQI GTAC 1 cut(s) 27
DdeI CTNAG 3 cut(s) 327, 366, 395
DpnI GATC 3 cut(s) 144, 451, 524
DpnII GATC 3 cut(s) 142, 449, 522
Eam1104I CTCTTC 1 cut(s) 418
EarI CTCTTC 1 cut(s) 418
Eco130I CCWWGG 1 cut(s) 301
Eco31I GGTCTC 1 cut(s) 146
EcoT14I CCWWGG 1 cut(s) 301
ErhI CCWWGG 1 cut(s) 301
FaeI CATG 3 cut(s) 271, 305, 556
FaiI YATR 5 cut(s) 269, 303, 314, 554, 571
FalI AAGNNNNNCTT 2 cut(s) 388, 420
FaqI GGGAC 1 cut(s) 59
FatI CATG 3 cut(s) 267, 301, 552
Fnu4HI GCNGC 2 cut(s) 101, 318
FokI GGATG 1 cut(s) 173
Fsp4HI GCNGC 2 cut(s) 101, 318
GluI GCNGC 2 cut(s) 101, 318
HaeIII GGCC 2 cut(s) 132, 216
HapII CCGG 1 cut(s) 129
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 3 cut(s) 271, 305, 556
HinfI GANTC 1 cut(s) 297
HpaII CCGG 1 cut(s) 129
HphI GGTGA 2 cut(s) 214, 448
Hpy188I TCNGA 2 cut(s) 67, 462
Hpy188III TCNNGA 2 cut(s) 140, 505
HpyAV CCTTC 2 cut(s) 273, 553
HpyCH4III ACNGT 2 cut(s) 358, 481
HpyCH4IV ACGT 1 cut(s) 72
HpyCH4V TGCA 4 cut(s) 51, 103, 350, 431
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 268, 317, 323
HpyF3I CTNAG 3 cut(s) 327, 366, 395
HpySE526I ACGT 1 cut(s) 72
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 3 cut(s) 271, 305, 556
Kzo9I GATC 3 cut(s) 142, 449, 522
LmnI GCTCC 3 cut(s) 308, 376, 424
Lsp1109I GCAGC 2 cut(s) 87, 304
LweI GCATC 2 cut(s) 195, 408
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 1 cut(s) 220
MalI GATC 3 cut(s) 144, 451, 524
MboI GATC 3 cut(s) 142, 449, 522
MboII GAAGA 3 cut(s) 266, 405, 480
MflI RGATCY 1 cut(s) 449
MluCI AATT 2 cut(s) 60, 566
MnlI CCTC 9 cut(s) 31, 67, 185, 206, 209, 299, 322, 361, 427
MseI TTAA 2 cut(s) 192, 240
MspCI CTTAAG 1 cut(s) 191
MspI CCGG 1 cut(s) 129
Mva1269I GAATGC 1 cut(s) 433
MwoI GCNNNNNNNGC 4 cut(s) 53, 268, 317, 323
NcoI CCATGG 1 cut(s) 301
NdeII GATC 3 cut(s) 142, 449, 522
NlaIII CATG 3 cut(s) 271, 305, 556
NmuCI GTSAC 1 cut(s) 220
NspI RCATGY 1 cut(s) 556
NspV TTCGAA 1 cut(s) 563
PaqCI CACCTGC 1 cut(s) 233
PctI GAATGC 1 cut(s) 433
PfeI GAWTC 1 cut(s) 297
PkrI GCNGC 2 cut(s) 102, 319
PspPI GGNCC 1 cut(s) 131
PsrI GAACNNNNNNTAC 2 cut(s) 509, 541
PsuI RGATCY 1 cut(s) 449
RsaI GTAC 1 cut(s) 28
RsaNI GTAC 1 cut(s) 27
SaqAI TTAA 2 cut(s) 192, 240
SatI GCNGC 2 cut(s) 101, 318
Sau3AI GATC 3 cut(s) 142, 449, 522
Sau96I GGNCC 1 cut(s) 131
SfaNI GCATC 2 cut(s) 195, 408
SfcI CTRYAG 2 cut(s) 312, 453
SfuI TTCGAA 1 cut(s) 563
SmlI CTYRAG 2 cut(s) 191, 503
SmoI CTYRAG 2 cut(s) 191, 503
Sse9I AATT 2 cut(s) 60, 566
StyI CCWWGG 1 cut(s) 301
TaaI ACNGT 2 cut(s) 358, 481
TaiI ACGT 1 cut(s) 75
TaqI TCGA 1 cut(s) 563
TasI AATT 2 cut(s) 60, 566
TfiI GAWTC 1 cut(s) 297
Tru1I TTAA 2 cut(s) 192, 240
Tru9I TTAA 2 cut(s) 192, 240
TscAI CASTG 1 cut(s) 152
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 2 cut(s) 100, 317
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 1 cut(s) 440
TspGWI ACGGA 1 cut(s) 226
TspRI CASTG 1 cut(s) 152
Vha464I CTTAAG 1 cut(s) 191
XceI RCATGY 1 cut(s) 556
ZraI GACGTC 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.