MD07G1126000.v1.1
ERF Family

Thioesterase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002384
Physical Location & Seq
Forward (+)
17784075 .. 17786394
2320 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1126000.v1.1.491

Sequence Viewer

Length: 540 bp
ATGGTGAAACCCTCCATTGAAATCACAGAGGACTTCGCTTCCGCAGCAAAAATTGCAGACGACATTCCTCGGGAAGACGTAATCAGAGTTCTGAATTTTCTACAAGGAGTTGGGATCTCGGACCCCGTGCCAGATGAGTTCGATGCTAGAAAGGATACCTACTCCGACATCATTCGTGACCTCCTGAAGCCCCTTCACATATCCCGTGGTCGTGTCACCTGCCTCGTATCTGTGAAACCTGCTGTCACTAATTTCTATTCCGGATTTCATGGAGGAGCAATTGCTGCTGTTGCTGAGGCTGTGTCTGTAGCTTGTGCAAGAACCGTCGTGGCTAATGATAAGGAGCTTTTTCTGGGGGAACTGAGCGTTTCTTACCTCTCTAGTGCAACAAAGAATGAAGAGGTGATAGTTGACTCATCTGTTGTTCGGAGTGGAAGAAATCTGAGCGTGATAGCTCTTGAATTGAAACTCAAGAAAACCGGGCGCTTGGTTTACACTGCTCGTGCTACGTTCTACCATACGCCTGTTGCAAAATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4HBT

Protein Analysis

180

Amino Acids

19.41

Weight (kDa)

6.83

Isoelectric Point (pI)

28.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HBT_3 PF13622 88 - 171 2e-07 Acyl-CoA thioesterase N-terminal domain
4HBT PF03061 90 - 161 1.4e-08 Thioesterase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 227
Acc36I ACCTGC 2 cut(s) 227, 247
AccIII TCCGGA 1 cut(s) 260
AciI CCGC 1 cut(s) 42
AclWI GGATC 1 cut(s) 122
AcsI RAATTY 1 cut(s) 94
AcuI CTGAAG 1 cut(s) 206
AgsI TTSAA 3 cut(s) 20, 461, 466
AluBI AGCT 3 cut(s) 311, 346, 455
AluI AGCT 3 cut(s) 311, 346, 455
AlwI GGATC 1 cut(s) 122
Ama87I CYCGRG 1 cut(s) 69
Aor13HI TCCGGA 1 cut(s) 260
ApeKI GCWGC 2 cut(s) 44, 284
ApoI RAATTY 1 cut(s) 94
AspLEI GCGC 1 cut(s) 486
AspS9I GGNCC 1 cut(s) 121
AsuC2I CCSGG 1 cut(s) 481
AsuHPI GGTGA 3 cut(s) 16, 208, 415
AvaI CYCGRG 1 cut(s) 69
AvaII GGWCC 1 cut(s) 121
BauI CACGAG 1 cut(s) 501
BbsI GAAGAC 1 cut(s) 81
BbvCI CCTCAGC 1 cut(s) 294
BbvI GCAGC 2 cut(s) 56, 271
BciVI GTATCC 1 cut(s) 148
BcnI CCSGG 1 cut(s) 481
BfaI CTAG 2 cut(s) 147, 381
BfmI CTRYAG 1 cut(s) 306
BfoI RGCGCY 1 cut(s) 487
BfuAI ACCTGC 2 cut(s) 227, 247
BfuI GTATCC 1 cut(s) 148
BisI GCNGC 2 cut(s) 45, 285
BlsI GCNGC 2 cut(s) 46, 286
Bme1390I CCNGG 1 cut(s) 481
Bme18I GGWCC 1 cut(s) 121
BmeT110I CYCGRG 1 cut(s) 69
BmgT120I GGNCC 1 cut(s) 121
BmiI GGNNCC 1 cut(s) 123
BmrFI CCNGG 1 cut(s) 481
BmsI GCATC 1 cut(s) 133
BpiI GAAGAC 1 cut(s) 81
Bpu10I CCTNAGC 1 cut(s) 294
BpuEI CTTGAG 1 cut(s) 455
BpuMI CCSGG 1 cut(s) 481
BsaJI CCNNGG 2 cut(s) 68, 205
BsaWI WCCGGW 1 cut(s) 260
BseAI TCCGGA 1 cut(s) 260
BseDI CCNNGG 2 cut(s) 68, 205
BseMII CTCAG 3 cut(s) 285, 353, 434
BseRI GAGGAG 1 cut(s) 288
BseXI GCAGC 2 cut(s) 56, 271
BsiHKCI CYCGRG 1 cut(s) 69
BsiSI CCGG 2 cut(s) 261, 480
BsoBI CYCGRG 1 cut(s) 69
Bsp13I TCCGGA 1 cut(s) 260
Bsp143I GATC 1 cut(s) 114
BspACI CCGC 1 cut(s) 42
BspCNI CTCAG 3 cut(s) 286, 354, 435
BspEI TCCGGA 1 cut(s) 260
BspLI GGNNCC 1 cut(s) 123
BspMI ACCTGC 2 cut(s) 227, 247
BspPI GGATC 1 cut(s) 122
BssECI CCNNGG 2 cut(s) 68, 205
BssMI GATC 1 cut(s) 114
BssSI CACGAG 1 cut(s) 501
Bst2BI CACGAG 1 cut(s) 501
Bst4CI ACNGT 1 cut(s) 325
Bst6I CTCTTC 1 cut(s) 393
BstAPI GCANNNNNTGC 2 cut(s) 53, 284
BstDEI CTNAG 3 cut(s) 294, 362, 443
BstDSI CCRYGG 1 cut(s) 205
BstH2I RGCGCY 1 cut(s) 487
BstHHI GCGC 1 cut(s) 486
BstKTI GATC 1 cut(s) 117
BstMBI GATC 1 cut(s) 114
BstMWI GCNNNNNNNGC 4 cut(s) 44, 53, 284, 290
BstSCI CCNGG 1 cut(s) 479
BstSFI CTRYAG 1 cut(s) 306
BstV1I GCAGC 2 cut(s) 56, 271
BstV2I GAAGAC 1 cut(s) 81
BstX2I RGATCY 1 cut(s) 114
BstYI RGATCY 1 cut(s) 114
BsuI GTATCC 1 cut(s) 148
BtgI CCRYGG 1 cut(s) 205
BtsI GCAGTG 1 cut(s) 495
BtsIMutI CAGTG 1 cut(s) 495
BveI ACCTGC 2 cut(s) 227, 247
CfoI GCGC 1 cut(s) 486
Cfr13I GGNCC 1 cut(s) 121
CviAII CATG 1 cut(s) 269
CviJI RGCY 6 cut(s) 190, 299, 311, 332, 346, 455
CviKI_1 RGCY 6 cut(s) 190, 299, 311, 332, 346, 455
DdeI CTNAG 3 cut(s) 294, 362, 443
DpnI GATC 1 cut(s) 116
DpnII GATC 1 cut(s) 114
Eam1104I CTCTTC 1 cut(s) 393
EarI CTCTTC 1 cut(s) 393
Eco47I GGWCC 1 cut(s) 121
Eco57I CTGAAG 1 cut(s) 206
Eco88I CYCGRG 1 cut(s) 69
FaeI CATG 1 cut(s) 272
FaiI YATR 4 cut(s) 200, 270, 519, 538
FatI CATG 1 cut(s) 268
Fnu4HI GCNGC 2 cut(s) 45, 285
Fsp4HI GCNGC 2 cut(s) 45, 285
FspBI CTAG 2 cut(s) 147, 381
GlaI GCGC 1 cut(s) 485
GluI GCNGC 2 cut(s) 45, 285
HaeII RGCGCY 1 cut(s) 487
HapII CCGG 2 cut(s) 261, 480
HhaI GCGC 1 cut(s) 486
Hin1II CATG 1 cut(s) 272
Hin6I GCGC 1 cut(s) 484
HinP1I GCGC 1 cut(s) 484
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HinfI GANTC 1 cut(s) 413
HpaII CCGG 2 cut(s) 261, 480
HphI GGTGA 3 cut(s) 16, 208, 415
Hpy166II GTNNAC 2 cut(s) 412, 493
Hpy188I TCNGA 6 cut(s) 86, 93, 121, 166, 429, 444
Hpy188III TCNNGA 6 cut(s) 71, 176, 184, 261, 458, 472
Hpy8I GTNNAC 2 cut(s) 412, 493
Hpy99I CGWCG 1 cut(s) 329
HpyAV CCTTC 1 cut(s) 203
HpyCH4III ACNGT 1 cut(s) 325
HpyCH4IV ACGT 2 cut(s) 78, 509
HpyCH4V TGCA 4 cut(s) 56, 317, 386, 530
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 53, 284, 290
HpyF3I CTNAG 3 cut(s) 294, 362, 443
HpySE526I ACGT 2 cut(s) 78, 509
Hsp92II CATG 1 cut(s) 272
HspAI GCGC 1 cut(s) 484
Kpn2I TCCGGA 1 cut(s) 260
Kzo9I GATC 1 cut(s) 114
LmnI GCTCC 2 cut(s) 275, 343
LpnPI CCDG 7 cut(s) 144, 197, 232, 252, 274, 338, 493
Lsp1109I GCAGC 2 cut(s) 56, 271
LweI GCATC 1 cut(s) 133
MaeI CTAG 2 cut(s) 147, 381
MaeII ACGT 2 cut(s) 78, 509
MaeIII GTNAC 3 cut(s) 176, 214, 244
MalI GATC 1 cut(s) 116
MboI GATC 1 cut(s) 114
MboII GAAGA 3 cut(s) 86, 410, 447
MfeI CAATTG 1 cut(s) 279
MflI RGATCY 1 cut(s) 114
MluCI AATT 6 cut(s) 51, 94, 250, 279, 461, 533
MlyI GAGTC 1 cut(s) 407
MmeI TCCRAC 1 cut(s) 189
MnlI CCTC 9 cut(s) 22, 22, 78, 191, 233, 266, 289, 386, 394
MroI TCCGGA 1 cut(s) 260
MspI CCGG 2 cut(s) 261, 480
MspR9I CCNGG 1 cut(s) 481
MunI CAATTG 1 cut(s) 279
MwoI GCNNNNNNNGC 4 cut(s) 44, 53, 284, 290
NciI CCSGG 1 cut(s) 481
NdeII GATC 1 cut(s) 114
NlaIII CATG 1 cut(s) 272
NlaIV GGNNCC 1 cut(s) 123
NmuCI GTSAC 3 cut(s) 176, 214, 244
PaqCI CACCTGC 1 cut(s) 227
PkrI GCNGC 2 cut(s) 46, 286
PleI GAGTC 1 cut(s) 407
PpsI GAGTC 1 cut(s) 407
PspN4I GGNNCC 1 cut(s) 123
PspPI GGNCC 1 cut(s) 121
PsrI GAACNNNNNNTAC 2 cut(s) 72, 104
PsuI RGATCY 1 cut(s) 114
SatI GCNGC 2 cut(s) 45, 285
Sau3AI GATC 1 cut(s) 114
Sau96I GGNCC 1 cut(s) 121
SchI GAGTC 1 cut(s) 407
ScrFI CCNGG 1 cut(s) 481
SfaNI GCATC 1 cut(s) 133
SfcI CTRYAG 1 cut(s) 306
SinI GGWCC 1 cut(s) 121
SmlI CTYRAG 1 cut(s) 470
SmoI CTYRAG 1 cut(s) 470
Sse9I AATT 6 cut(s) 51, 94, 250, 279, 461, 533
SsiI CCGC 1 cut(s) 42
SspMI CTAG 2 cut(s) 147, 381
StyD4I CCNGG 1 cut(s) 479
TaaI ACNGT 1 cut(s) 325
TaiI ACGT 2 cut(s) 81, 512
TaqI TCGA 1 cut(s) 141
TasI AATT 6 cut(s) 51, 94, 250, 279, 461, 533
TscAI CASTG 1 cut(s) 502
TseFI GTSAC 3 cut(s) 176, 214, 244
TseI GCWGC 2 cut(s) 44, 284
Tsp45I GTSAC 3 cut(s) 176, 214, 244
TspDTI ATGAA 2 cut(s) 257, 411
TspRI CASTG 1 cut(s) 502
VpaK11BI GGWCC 1 cut(s) 121
XapI RAATTY 1 cut(s) 94
XspI CTAG 2 cut(s) 147, 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.