RLG00000028312
ERF Family

Thioesterase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0002384
Physical Location & Seq
Forward (+)
21323193 .. 21325299
2107 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028312

Sequence Viewer

Length: 537 bp
ATGGCAAAGCCCTGTGGTACTCTGGCAGAGAGGTTTGCTTGCACAGCCTCAATTTCAGATGACATCTCTGAACAACAGCTCAAGGATACGCTGCATTTTCTCAAACGAGTGGGTATCTCCGGCCCTGTTCCAGATCACTGCGAGACCAAAGACTTCTACTCCTACCTCGTTCGTGGCATCCTTAAGCCCCTCAACATTCAACGAGGCCGTGTCACCTGCCTCGTCTCCGTTAAACCTGCTTTTATCAACTCTTTTGGTGGATTCCATGGAGGAGCTATAGCTGCTGTTGCTGAGGCTGTGTTGGTAGCTACTGCGAGAACGGTTGTGGCTGAGGATAAGGAGCTTTTTCTTGGGGAACTAAGCATCTCTTACCTCTCTTCTGCTCCAAAGAATGCAGAGGTGATAGTTGATGGATCTGTAGTTCGGAGTGGAAGAAATCTTACTGTAATAGCACAGGAGTTCAAGCTCAAGAAAACTGGGAACTTGATCTACACTGCTCGTGCTACCTTCTATCACATGCCTGTTTCGAAATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4HBT

Protein Analysis

179

Amino Acids

19.26

Weight (kDa)

9.08

Isoelectric Point (pI)

29.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
4HBT_3 PF13622 84 - 170 1.3e-06 Acyl-CoA thioesterase N-terminal domain
4HBT PF03061 86 - 159 2.9e-09 Thioesterase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 224
Acc36I ACCTGC 2 cut(s) 224, 244
AclWI GGATC 1 cut(s) 421
AfaI GTAC 1 cut(s) 19
AflII CTTAAG 1 cut(s) 182
AgsI TTSAA 2 cut(s) 200, 463
AloI GAACNNNNNNTCC 2 cut(s) 405, 437
AluBI AGCT 6 cut(s) 79, 275, 281, 308, 343, 466
AluI AGCT 6 cut(s) 79, 275, 281, 308, 343, 466
Alw26I GTCTC 2 cut(s) 137, 229
AlwI GGATC 1 cut(s) 421
AoxI GGCC 2 cut(s) 121, 205
ApeKI GCWGC 2 cut(s) 91, 281
AspS9I GGNCC 1 cut(s) 122
AsuHPI GGTGA 2 cut(s) 205, 412
AsuII TTCGAA 1 cut(s) 527
BarI GAAGNNNNNNTAC 2 cut(s) 424, 456
BauI CACGAG 1 cut(s) 498
BbvCI CCTCAGC 2 cut(s) 291, 330
BbvI GCAGC 2 cut(s) 78, 268
BccI CCATC 1 cut(s) 404
BceAI ACGGC 1 cut(s) 192
BciVI GTATCC 1 cut(s) 79
BcoDI GTCTC 2 cut(s) 137, 229
BfmI CTRYAG 2 cut(s) 276, 417
BfrI CTTAAG 1 cut(s) 182
BfuAI ACCTGC 2 cut(s) 224, 244
BfuI GTATCC 1 cut(s) 79
BisI GCNGC 2 cut(s) 92, 282
BlsI GCNGC 2 cut(s) 93, 283
BmgT120I GGNCC 1 cut(s) 122
BmrI ACTGGG 1 cut(s) 486
BmsI GCATC 2 cut(s) 186, 372
BmuI ACTGGG 1 cut(s) 486
Bpu10I CCTNAGC 2 cut(s) 291, 330
Bpu14I TTCGAA 1 cut(s) 527
BpuEI CTTGAG 2 cut(s) 65, 452
BsaI GGTCTC 1 cut(s) 137
BsaJI CCNNGG 1 cut(s) 265
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bse1I ACTGG 1 cut(s) 481
BseDI CCNNGG 1 cut(s) 265
BseGI GGATG 1 cut(s) 177
BseMII CTCAG 2 cut(s) 282, 321
BseNI ACTGG 1 cut(s) 481
BseRI GAGGAG 1 cut(s) 285
BseXI GCAGC 2 cut(s) 78, 268
BshFI GGCC 2 cut(s) 123, 207
BsiSI CCGG 1 cut(s) 120
BsmAI GTCTC 2 cut(s) 137, 229
BsmBI CGTCTC 1 cut(s) 229
BsmI GAATGC 1 cut(s) 397
BsnI GGCC 2 cut(s) 123, 207
Bso31I GGTCTC 1 cut(s) 137
Bsp119I TTCGAA 1 cut(s) 527
Bsp143I GATC 3 cut(s) 133, 413, 486
Bsp19I CCATGG 1 cut(s) 265
BspANI GGCC 2 cut(s) 123, 207
BspCNI CTCAG 2 cut(s) 283, 322
BspMI ACCTGC 2 cut(s) 224, 244
BspPI GGATC 1 cut(s) 421
BspT104I TTCGAA 1 cut(s) 527
BspTI CTTAAG 1 cut(s) 182
BspTNI GGTCTC 1 cut(s) 137
BsrI ACTGG 1 cut(s) 481
BssECI CCNNGG 1 cut(s) 265
BssMI GATC 3 cut(s) 133, 413, 486
BssSI CACGAG 1 cut(s) 498
BssT1I CCWWGG 1 cut(s) 265
Bst2BI CACGAG 1 cut(s) 498
Bst4CI ACNGT 2 cut(s) 322, 445
Bst6I CTCTTC 1 cut(s) 382
BstAFI CTTAAG 1 cut(s) 182
BstBI TTCGAA 1 cut(s) 527
BstC8I GCNNGC 1 cut(s) 40
BstDEI CTNAG 3 cut(s) 291, 330, 359
BstDSI CCRYGG 1 cut(s) 265
BstF5I GGATG 1 cut(s) 177
BstKTI GATC 3 cut(s) 136, 416, 489
BstMAI GTCTC 2 cut(s) 137, 229
BstMBI GATC 3 cut(s) 133, 413, 486
BstMWI GCNNNNNNNGC 3 cut(s) 44, 281, 287
BstNSI RCATGY 1 cut(s) 520
BstSFI CTRYAG 2 cut(s) 276, 417
BstV1I GCAGC 2 cut(s) 78, 268
BstX2I RGATCY 1 cut(s) 413
BstYI RGATCY 1 cut(s) 413
BsuI GTATCC 1 cut(s) 79
BsuRI GGCC 2 cut(s) 123, 207
BtgI CCRYGG 1 cut(s) 265
BtsCI GGATG 1 cut(s) 177
BtsI GCAGTG 2 cut(s) 136, 492
BtsIMutI CAGTG 2 cut(s) 136, 492
BveI ACCTGC 2 cut(s) 224, 244
Cac8I GCNNGC 1 cut(s) 40
Cfr13I GGNCC 1 cut(s) 122
Csp6I GTAC 1 cut(s) 18
CviAII CATG 2 cut(s) 266, 517
CviQI GTAC 1 cut(s) 18
DdeI CTNAG 3 cut(s) 291, 330, 359
DpnI GATC 3 cut(s) 135, 415, 488
DpnII GATC 3 cut(s) 133, 413, 486
Eam1104I CTCTTC 1 cut(s) 382
EarI CTCTTC 1 cut(s) 382
Eco130I CCWWGG 1 cut(s) 265
Eco31I GGTCTC 1 cut(s) 137
EcoT14I CCWWGG 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 265
Esp3I CGTCTC 1 cut(s) 229
FaeI CATG 2 cut(s) 269, 520
FaiI YATR 4 cut(s) 267, 278, 518, 535
FalI AAGNNNNNCTT 2 cut(s) 352, 384
FatI CATG 2 cut(s) 265, 516
Fnu4HI GCNGC 2 cut(s) 92, 282
FokI GGATG 1 cut(s) 164
Fsp4HI GCNGC 2 cut(s) 92, 282
GluI GCNGC 2 cut(s) 92, 282
HaeIII GGCC 2 cut(s) 123, 207
HapII CCGG 1 cut(s) 120
Hin1II CATG 2 cut(s) 269, 520
HinfI GANTC 1 cut(s) 261
HpaII CCGG 1 cut(s) 120
HphI GGTGA 2 cut(s) 205, 412
Hpy188I TCNGA 3 cut(s) 58, 70, 426
Hpy188III TCNNGA 2 cut(s) 131, 469
HpyAV CCTTC 1 cut(s) 517
HpyCH4III ACNGT 2 cut(s) 322, 445
HpyCH4V TGCA 3 cut(s) 42, 94, 395
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 281, 287
HpyF3I CTNAG 3 cut(s) 291, 330, 359
Hsp92II CATG 2 cut(s) 269, 520
Kzo9I GATC 3 cut(s) 133, 413, 486
LmnI GCTCC 3 cut(s) 272, 340, 388
LpnPI CCDG 9 cut(s) 8, 25, 133, 138, 144, 229, 249, 440, 462
Lsp1109I GCAGC 2 cut(s) 78, 268
LweI GCATC 2 cut(s) 186, 372
MaeIII GTNAC 1 cut(s) 211
MalI GATC 3 cut(s) 135, 415, 488
MboI GATC 3 cut(s) 133, 413, 486
MboII GAAGA 2 cut(s) 369, 444
MflI RGATCY 1 cut(s) 413
MluCI AATT 2 cut(s) 51, 530
MseI TTAA 2 cut(s) 183, 231
MspCI CTTAAG 1 cut(s) 182
MspI CCGG 1 cut(s) 120
Mva1269I GAATGC 1 cut(s) 397
MwoI GCNNNNNNNGC 3 cut(s) 44, 281, 287
NcoI CCATGG 1 cut(s) 265
NdeII GATC 3 cut(s) 133, 413, 486
NlaIII CATG 2 cut(s) 269, 520
NmuCI GTSAC 1 cut(s) 211
NspI RCATGY 1 cut(s) 520
NspV TTCGAA 1 cut(s) 527
PaqCI CACCTGC 1 cut(s) 224
PctI GAATGC 1 cut(s) 397
PfeI GAWTC 1 cut(s) 261
PkrI GCNGC 2 cut(s) 93, 283
PspPI GGNCC 1 cut(s) 122
PsrI GAACNNNNNNTAC 2 cut(s) 473, 505
PsuI RGATCY 1 cut(s) 413
RsaI GTAC 1 cut(s) 19
RsaNI GTAC 1 cut(s) 18
SaqAI TTAA 2 cut(s) 183, 231
SatI GCNGC 2 cut(s) 92, 282
Sau3AI GATC 3 cut(s) 133, 413, 486
Sau96I GGNCC 1 cut(s) 122
SfaNI GCATC 2 cut(s) 186, 372
SfcI CTRYAG 2 cut(s) 276, 417
SfuI TTCGAA 1 cut(s) 527
SmlI CTYRAG 3 cut(s) 80, 182, 467
SmoI CTYRAG 3 cut(s) 80, 182, 467
Sse9I AATT 2 cut(s) 51, 530
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 2 cut(s) 322, 445
TaqI TCGA 1 cut(s) 527
TasI AATT 2 cut(s) 51, 530
TfiI GAWTC 1 cut(s) 261
Tru1I TTAA 2 cut(s) 183, 231
Tru9I TTAA 2 cut(s) 183, 231
TscAI CASTG 2 cut(s) 143, 499
TseFI GTSAC 1 cut(s) 211
TseI GCWGC 2 cut(s) 91, 281
Tsp45I GTSAC 1 cut(s) 211
TspGWI ACGGA 1 cut(s) 217
TspRI CASTG 2 cut(s) 143, 499
Vha464I CTTAAG 1 cut(s) 182
XceI RCATGY 1 cut(s) 520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.