Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb7

Orthogroup ID

OG0001152

Physical Location & Seq

Genomic Coordinates Forward (+)

19355659 .. 19358181

Gene Structure 2523 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_7g25290.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2079 bp

ATGGGTATAATGGGCATTATATTCTCCATCTTCTTAACTGCAATAAAACTGGACGTGTGCATGATGAAAAGGACAGGGCTTTCTTTGAAGATTGGTGCTCCAGGGTTCATTTTCACCCTTGCGGTATCCTTAGGCCTCGGAATCCCACTAGCCAGCTCTATTCCTGGAATGCCAGGAGGAATTCTCCCCCTTTTTTTGTTTACTATGCTTTCTTTCAGTTTCTCCCCTGGTGTAGCTCAGGCCTTAGACGACCTCAACCTTATGAACTCTGAGTTGGGCCAACTCGCCATGTTCTCTGTTTTGCTCGTTGAAATCATACAATGGTTGATCTCAACGGCACAAGACATAGCTACTAAAGATAATGTAAGCCACGGAGCTTTGACCTTTCTTTCGGTAGTGATTATGGCTCTGGTGATTATCTTTGCCATACGCCCGCTTATGGTATGGATTGTTAAGTCCACACCAGAAGGACAAGAAGTAAAGGAGGTTTATGTAGTAGCAATACTACTTGGTGCACTAATCCTAGCGTTTGTATCCGACGCAATAGGGTGCTTCTTCATTTTAGGTCCTTTTCTGCTTGGGTTAGTCATACCAGACGGACCGCCTTTGGGTGCAGCAATAATAGCCAAGACTGAATTCTTGGTCTCTGAATGTCTTCTTCCAATCTTCTTTCTTCGTATCGGCTTCTTCTCAGACATACTTACTATTTCAAATTGGAAGAGTTTTACCACATTGCAGGCCATCATCGGTGCCGCATATCTGGCAAAGTTGTTCACAGTCACCGTGGCTGCATTGTGCTGCAAAATTAGTTTGAGAAATAGTCTCTTGCTCGGTGTGATGATGAATATCAGGGGTATTATAGAAGTAGTCATTTTTTCTCGATGGAGGCACACTGAGGTCTTAGATGACCAATTTTATACCCAACTACTATTATCTGTGCTGATTATGAACATGATTTCAACATCATTGTTACATTTCACGTACAGGGCTCATCTTAGACTTGGGCCATCACTCAAAGGTCCTCGCATTAAAAACATCCAATCAGTTCAAAGAAACTATCCTTTCGACATTCTTTGTTGTATCCACAACGAGCAAACTATCCGCAACCTCACCTCCATCCTAGAAGCCTCAAACCCAACAGAAGAGAGCCCCATTTGCGCCTACATTATCCAAGCAGTCGAGCTAGTGGGGAGGGCTATACCTGTGCTAGTCCCTTACAAGAAGAAGAAAAAGGGGAAAAAGTGTAGCCGATTAAATTCACTCACACATCAACTGATGCAAGCCTTCAAGAACTACTCGGAGAACTCAAGAGGACCTGTCTTGATCGAATCATTCACTATGATCGCTCCCTATAAATTGATGCATGAGACTATTTGCCGTCTAGTGGATGACAATCACGTACCTTTGGTTATCCTCCCATTTCATGATAACCCCTGGATCTGCAACACCGTGACTACTGCAATACGCCAATTCAATAAAAATATGCTGCGATATTCATCTTCTACAGTTGGAATACTAGTCGAAAGAGGGTTCCCCGGTCCGGTGACCATGTCCCATTTCTCATATAATGTGGCTGCATTTTTCATTGGCGGACCAGATGATCGCGAGGCTTTGGCTTTAGCAGCACGTATGTTAGGCAACCAAAATGTGGCCGTCACAATATACAGGATTCGTATTATGCACCAGGGTAACTATAATGAAGACGAGGAGAATGAAGCTAGGTTGGATGATGCAATGGTGGATGAGTTTAAGCTGAGCAGTATTGGGAATGATTGCCTTAAATGGCGAGATATCGAGGTTGAGGACACTGAACAGCTTATGGATGCAATTATGGATTCGCAAGGGGATTATGATCTTGTAATGGTCGGAAGGCGGCACGCAGACAATTCATTGAGGGATGAGGAAATGTCAGAGTTTGTGCATTATGCGGAGCTGGGAATGATCGGCGACATGCTTGCTTCTTCAGATTTCTGTGGTGGAATGGTGAATGTTTTGGTGCTGCAAGAAAGTACGGAACTTAGTAAAAATTATGGATCGACTCATGGGCGTGACGTCTCACAAAATTTAGGATTGAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0042592 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

693

Amino Acids

76.71

Weight (kDa)

5.75

Isoelectric Point (pI)

38.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	5 - 325	2.5e-24	Sodium/hydrogen exchanger, transmembrane
CHX17_C	PF23259	522 - 671	9.4e-07	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001152)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g43690 FvH4_7g25211 FvH4_7g25250 FvH4_7g25290 FvH4_7g25310
malus_domestica	MD01G1158700.v1.1 MD01G1158800.v1.1 MD07G1227200.v1.1 MD07G1227600.v1.1
prunus_persica	Prupe.2G257000_v2.0.a1 Prupe.2G257100_v2.0.a1
pyrus_communis	pycom01g17540
rosa_chinensis	RchiOBHm_Chr1g0371191 RchiOBHm_Chr1g0371201 RchiOBHm_Chr1g0371211
rosa_laevigata	RLG00000026978 RLG00000026979 RLG00000026980 RLG00000026981 RLG00000026983 RLG00000036771
rosa_multiflora	Rmu_co8222838.1_g000001 Rmu_sc0000631.1_g000023 Rmu_sc0001018.1_g000042 Rmu_sc0001018.1_g000044 Rmu_sc0001018.1_g000049 Rmu_sc0001018.1_g000050 Rmu_sc0001018.1_g000051 Rmu_sc0008081.1_g000003
rosa_roxburghii	Rroxscaffold_4G00285900 Rroxscaffold_4G00285910
rosa_rugosa	Rorug01G0361700 Rorug01G0361900 Rorug01G0362000 Rorug01G0362200 Rorug05G0460600
rosa_samantha	Rh1AG372000 Rh1AG372200 Rh1AG372300 Rh1BG335100 Rh1BG335500 Rh1BG335700 Rh1BG335800 Rh1CG348600 Rh1CG349000 Rh1CG349100 Rh1CG349200 Rh1DG366500 Rh1DG366900 Rh1DG367000 Rh5BG537300 Rh5CG560800 Rh5DG548900
rosa_wichuraiana	Rw1G032720 Rw1G032730 Rw1G032740 Rw5G047750 Rw5G047880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	2055
AccB1I	GGYRCC	1 cut(s)	749
AccB7I	CCANNNNNTGG	1 cut(s)	1648
AccII	CGCG	1 cut(s)	1605
AciI	CCGC	8 cut(s)	122, 434, 602, 753, 1102, 1590, 1873, 1928
AclWI	GGATC	2 cut(s)	1445, 2041
AcoI	YGGCCR	1 cut(s)	1650
AcsI	RAATTY	4 cut(s)	180, 635, 1255, 2062
AcuI	CTGAAG	1 cut(s)	1947
AcyI	GRCGYC	1 cut(s)	2052
AfaI	GTAC	3 cut(s)	983, 1401, 2011
AfiI	CCNNNNNNNGG	5 cut(s)	439, 608, 1384, 1540, 1648
AflIII	ACRYGT	1 cut(s)	54
AgsI	TTSAA	8 cut(s)	88, 311, 711, 960, 1049, 1288, 1474, 2074
AhlI	ACTAGT	1 cut(s)	1516
AjiI	CACGTC	1 cut(s)	55
AjnI	CCWGG	6 cut(s)	100, 163, 172, 226, 1433, 1683
AjuI	GAANNNNNNNTTGG	4 cut(s)	257, 289, 620, 652
AluBI	AGCT	9 cut(s)	156, 236, 350, 377, 1183, 1718, 1753, 1816, 1933
AluI	AGCT	9 cut(s)	156, 236, 350, 377, 1183, 1718, 1753, 1816, 1933
Alw21I	GWGCWC	2 cut(s)	100, 517
Alw26I	GTCTC	4 cut(s)	649, 827, 1361, 2059
Alw44I	GTGCAC	1 cut(s)	513
AlwI	GGATC	2 cut(s)	1445, 2041
AoxI	GGCC	6 cut(s)	133, 240, 277, 738, 1004, 1650
ApaLI	GTGCAC	1 cut(s)	513
ApeKI	GCWGC	7 cut(s)	614, 788, 798, 1486, 1574, 1622, 1999
ApoI	RAATTY	4 cut(s)	180, 635, 1255, 2062
ArsI	GACNNNNNNTTYG	2 cut(s)	373, 405
Asp700I	GAANNNNTTC	1 cut(s)	654
AspLEI	GCGC	1 cut(s)	1160
AspS9I	GGNCC	8 cut(s)	277, 566, 599, 1004, 1019, 1313, 1538, 1592
AsuC2I	CCSGG	1 cut(s)	1536
AsuHPI	GGTGA	6 cut(s)	106, 424, 772, 1102, 1555, 1996
AvaII	GGWCC	6 cut(s)	566, 599, 1019, 1313, 1538, 1592
AxyI	CCTNAGG	1 cut(s)	130
BaeGI	GKGCMC	1 cut(s)	517
BanI	GGYRCC	1 cut(s)	749
BanII	GRGCYC	2 cut(s)	991, 1151
BbsI	GAAGAC	2 cut(s)	647, 1707
Bbv12I	GWGCWC	2 cut(s)	100, 517
BbvI	GCAGC	7 cut(s)	626, 775, 785, 1473, 1561, 1634, 1986
BccI	CCATC	5 cut(s)	35, 749, 876, 1015, 1124
BceAI	ACGGC	3 cut(s)	351, 1362, 1637
BcgI	CGANNNNNNTGC	2 cut(s)	1937, 1971
BciT130I	CCWGG	6 cut(s)	102, 165, 174, 228, 1435, 1685
BciVI	GTATCC	3 cut(s)	136, 544, 1091
BcnI	CCSGG	1 cut(s)	1536
BcoDI	GTCTC	4 cut(s)	649, 827, 1361, 2059
BcuI	ACTAGT	1 cut(s)	1516
BfaI	CTAG	8 cut(s)	149, 524, 1121, 1184, 1208, 1382, 1517, 1719
BfmI	CTRYAG	1 cut(s)	1503
BfuI	GTATCC	3 cut(s)	136, 544, 1091
BisI	GCNGC	9 cut(s)	615, 753, 789, 799, 1487, 1575, 1623, 1874, 2000
BlpI	GCTNAGC	1 cut(s)	1754
BlsI	GCNGC	9 cut(s)	616, 754, 790, 800, 1488, 1576, 1624, 1875, 2001
Bme1390I	CCNGG	7 cut(s)	102, 165, 174, 228, 1435, 1536, 1685
Bme18I	GGWCC	6 cut(s)	566, 599, 1019, 1313, 1538, 1592
BmgBI	CACGTC	1 cut(s)	55
BmgT120I	GGNCC	8 cut(s)	277, 566, 599, 1004, 1019, 1313, 1538, 1592
BmiI	GGNNCC	2 cut(s)	751, 1532
BmrFI	CCNGG	7 cut(s)	102, 165, 174, 228, 1435, 1536, 1685
BmsI	GCATC	4 cut(s)	1266, 1350, 1720, 1813
BpiI	GAAGAC	2 cut(s)	647, 1707
BplI	GAGNNNNNCTC	2 cut(s)	168, 200
BpmI	CTGGAG	1 cut(s)	84
Bpu10I	CCTNAGC	1 cut(s)	237
Bpu1102I	GCTNAGC	1 cut(s)	1754
BpuEI	CTTGAG	1 cut(s)	1291
BpuMI	CCSGG	1 cut(s)	1536
BsaAI	YACGTR	3 cut(s)	981, 1399, 1628
BsaBI	GATNNNNATC	4 cut(s)	845, 1392, 1495, 1851
BsaHI	GRCGYC	1 cut(s)	2052
BsaI	GGTCTC	1 cut(s)	649
BsaJI	CCNNGG	8 cut(s)	101, 136, 226, 370, 783, 1433, 1534, 1684
BsaWI	WCCGGW	1 cut(s)	1540
BsaXI	ACNNNNNCTCC	2 cut(s)	1097, 1127
Bsc4I	CCNNNNNNNGG	5 cut(s)	439, 608, 1384, 1540, 1648
Bse1I	ACTGG	1 cut(s)	54
Bse21I	CCTNAGG	1 cut(s)	130
Bse3DI	GCAATG	2 cut(s)	731, 1740
Bse8I	GATNNNNATC	4 cut(s)	845, 1392, 1495, 1851
BseBI	CCWGG	6 cut(s)	102, 165, 174, 228, 1435, 1685
BseDI	CCNNGG	8 cut(s)	101, 136, 226, 370, 783, 1433, 1534, 1684
BseGI	GGATG	7 cut(s)	1035, 1116, 1393, 1732, 1747, 1828, 1903
BseJI	GATNNNNATC	4 cut(s)	845, 1392, 1495, 1851
BseLI	CCNNNNNNNGG	5 cut(s)	439, 608, 1384, 1540, 1648
BseMI	GCAATG	2 cut(s)	731, 1740
BseMII	CTCAG	5 cut(s)	251, 261, 705, 885, 1745
BseNI	ACTGG	1 cut(s)	54
BseRI	GAGGAG	1 cut(s)	1721
BseSI	GKGCMC	1 cut(s)	517
BseXI	GCAGC	7 cut(s)	626, 775, 785, 1473, 1561, 1634, 1986
BseYI	CCCAGC	1 cut(s)	1933
BsgI	GTGCAG	1 cut(s)	633
Bsh1236I	CGCG	1 cut(s)	1605
BshFI	GGCC	6 cut(s)	135, 242, 279, 740, 1006, 1652
BshNI	GGYRCC	1 cut(s)	749
BsiHKAI	GWGCWC	2 cut(s)	100, 517
BsiSI	CCGG	2 cut(s)	1536, 1541
BslFI	GGGAC	2 cut(s)	1196, 1537
BslI	CCNNNNNNNGG	5 cut(s)	439, 608, 1384, 1540, 1648
BsmAI	GTCTC	4 cut(s)	649, 827, 1361, 2059
BsmBI	CGTCTC	1 cut(s)	2059
BsmFI	GGGAC	2 cut(s)	1196, 1537
BsmI	GAATGC	1 cut(s)	174
BsnI	GGCC	6 cut(s)	135, 242, 279, 740, 1006, 1652
Bso31I	GGTCTC	1 cut(s)	649
Bsp1286I	GDGCHC	4 cut(s)	100, 517, 991, 1151
Bsp143I	GATC	8 cut(s)	327, 1323, 1341, 1437, 1600, 1852, 1941, 2033
Bsp1720I	GCTNAGC	1 cut(s)	1754
Bsp68I	TCGCGA	1 cut(s)	1605
BspACI	CCGC	8 cut(s)	122, 434, 602, 753, 1102, 1590, 1873, 1928
BspANI	GGCC	6 cut(s)	135, 242, 279, 740, 1006, 1652
BspCNI	CTCAG	5 cut(s)	250, 262, 704, 886, 1746
BspFNI	CGCG	1 cut(s)	1605
BspHI	TCATGA	1 cut(s)	1423
BspLI	GGNNCC	2 cut(s)	751, 1532
BspPI	GGATC	2 cut(s)	1445, 2041
BspT107I	GGYRCC	1 cut(s)	749
BspTNI	GGTCTC	1 cut(s)	649
BsrDI	GCAATG	2 cut(s)	731, 1740
BsrI	ACTGG	1 cut(s)	54
BssECI	CCNNGG	8 cut(s)	101, 136, 226, 370, 783, 1433, 1534, 1684
BssMI	GATC	8 cut(s)	327, 1323, 1341, 1437, 1600, 1852, 1941, 2033
BssNI	GRCGYC	1 cut(s)	2052
Bst2UI	CCWGG	6 cut(s)	102, 165, 174, 228, 1435, 1685
Bst4CI	ACNGT	4 cut(s)	778, 784, 1450, 1507
Bst6I	CTCTTC	2 cut(s)	713, 1137
BstACI	GRCGYC	1 cut(s)	2052
BstBAI	YACGTR	3 cut(s)	981, 1399, 1628
BstC8I	GCNNGC	6 cut(s)	154, 434, 738, 1281, 1878, 1956
BstDSI	CCRYGG	2 cut(s)	370, 783
BstEII	GGTNACC	1 cut(s)	1543
BstF5I	GGATG	7 cut(s)	1035, 1116, 1393, 1732, 1747, 1828, 1903
BstFNI	CGCG	1 cut(s)	1605
BstHHI	GCGC	1 cut(s)	1160
BstKTI	GATC	8 cut(s)	330, 1326, 1344, 1440, 1603, 1855, 1944, 2036
BstMAI	GTCTC	4 cut(s)	649, 827, 1361, 2059
BstMBI	GATC	8 cut(s)	327, 1323, 1341, 1437, 1600, 1852, 1941, 2033
BstMWI	GCNNNNNNNGC	4 cut(s)	623, 761, 1155, 1622
BstNI	CCWGG	6 cut(s)	102, 165, 174, 228, 1435, 1685
BstNSI	RCATGY	1 cut(s)	1954
BstPI	GGTNACC	1 cut(s)	1543
BstSCI	CCNGG	7 cut(s)	100, 163, 172, 226, 1433, 1534, 1683
BstSFI	CTRYAG	1 cut(s)	1503
BstSLI	GKGCMC	1 cut(s)	517
BstUI	CGCG	1 cut(s)	1605
BstV1I	GCAGC	7 cut(s)	626, 775, 785, 1473, 1561, 1634, 1986
BstV2I	GAAGAC	2 cut(s)	647, 1707
BstX2I	RGATCY	1 cut(s)	1437
BstYI	RGATCY	1 cut(s)	1437
Bsu36I	CCTNAGG	1 cut(s)	130
BsuI	GTATCC	3 cut(s)	136, 544, 1091
BsuRI	GGCC	6 cut(s)	135, 242, 279, 740, 1006, 1652
BtgI	CCRYGG	2 cut(s)	370, 783
BtrI	CACGTC	1 cut(s)	55
BtsCI	GGATG	7 cut(s)	1035, 1116, 1393, 1732, 1747, 1828, 1903
BtsIMutI	CAGTG	2 cut(s)	891, 1806
BtuMI	TCGCGA	1 cut(s)	1605
Cac8I	GCNNGC	6 cut(s)	154, 434, 738, 1281, 1878, 1956
CciI	TCATGA	1 cut(s)	1423
CfoI	GCGC	1 cut(s)	1160
Cfr13I	GGNCC	8 cut(s)	277, 566, 599, 1004, 1019, 1313, 1538, 1592
CpoI	CGGWCCG	2 cut(s)	599, 1538
CseI	GACGC	1 cut(s)	548
Csp6I	GTAC	3 cut(s)	982, 1400, 2010
CspI	CGGWCCG	2 cut(s)	599, 1538
CviAII	CATG	8 cut(s)	61, 289, 952, 1364, 1424, 1549, 1951, 2042
CviQI	GTAC	3 cut(s)	982, 1400, 2010
DpnI	GATC	8 cut(s)	329, 1325, 1343, 1439, 1602, 1854, 1943, 2035
DpnII	GATC	8 cut(s)	327, 1323, 1341, 1437, 1600, 1852, 1941, 2033
EaeI	YGGCCR	1 cut(s)	1650
Eam1104I	CTCTTC	2 cut(s)	713, 1137
EarI	CTCTTC	2 cut(s)	713, 1137
EciI	GGCGGA	1 cut(s)	1605
Eco147I	AGGCCT	2 cut(s)	135, 242
Eco24I	GRGCYC	2 cut(s)	991, 1151
Eco31I	GGTCTC	1 cut(s)	649
Eco32I	GATATC	1 cut(s)	1792
Eco47I	GGWCC	6 cut(s)	566, 599, 1019, 1313, 1538, 1592
Eco57I	CTGAAG	1 cut(s)	1947
Eco81I	CCTNAGG	1 cut(s)	130
Eco91I	GGTNACC	1 cut(s)	1543
EcoO109I	RGGNCCY	3 cut(s)	566, 1019, 1313
EcoO65I	GGTNACC	1 cut(s)	1543
EcoRI	GAATTC	2 cut(s)	180, 635
EcoRII	CCWGG	6 cut(s)	100, 163, 172, 226, 1433, 1683
EcoRV	GATATC	1 cut(s)	1792
EcoT22I	ATGCAT	1 cut(s)	1365
EcoT38I	GRGCYC	2 cut(s)	991, 1151
Esp3I	CGTCTC	1 cut(s)	2059
FaeI	CATG	8 cut(s)	64, 292, 955, 1367, 1427, 1552, 1954, 2045
FaqI	GGGAC	2 cut(s)	1196, 1537
FatI	CATG	8 cut(s)	60, 288, 951, 1363, 1423, 1548, 1950, 2041
FauI	CCCGC	1 cut(s)	441
Fnu4HI	GCNGC	9 cut(s)	615, 753, 789, 799, 1487, 1575, 1623, 1874, 2000
FokI	GGATG	7 cut(s)	1022, 1103, 1400, 1739, 1754, 1835, 1910
FriOI	GRGCYC	2 cut(s)	991, 1151
Fsp4HI	GCNGC	9 cut(s)	615, 753, 789, 799, 1487, 1575, 1623, 1874, 2000
FspBI	CTAG	8 cut(s)	149, 524, 1121, 1184, 1208, 1382, 1517, 1719
GlaI	GCGC	1 cut(s)	1159
GluI	GCNGC	9 cut(s)	615, 753, 789, 799, 1487, 1575, 1623, 1874, 2000
GsaI	CCCAGC	1 cut(s)	1937
GsuI	CTGGAG	1 cut(s)	84
HaeIII	GGCC	6 cut(s)	135, 242, 279, 740, 1006, 1652
HapII	CCGG	2 cut(s)	1536, 1541
HgaI	GACGC	1 cut(s)	548
HhaI	GCGC	1 cut(s)	1160
Hin1I	GRCGYC	1 cut(s)	2052
Hin1II	CATG	8 cut(s)	64, 292, 955, 1367, 1427, 1552, 1954, 2045
Hin6I	GCGC	1 cut(s)	1158
HinP1I	GCGC	1 cut(s)	1158
HinfI	GANTC	5 cut(s)	141, 1328, 1669, 1835, 2038
HpaII	CCGG	2 cut(s)	1536, 1541
HphI	GGTGA	6 cut(s)	106, 424, 772, 1102, 1555, 1996
Hpy166II	GTNNAC	4 cut(s)	201, 459, 515, 774
Hpy188I	TCNGA	9 cut(s)	140, 271, 538, 649, 694, 1300, 1868, 1912, 1966
Hpy188III	TCNNGA	6 cut(s)	879, 1288, 1308, 1321, 1424, 1604
Hpy8I	GTNNAC	4 cut(s)	201, 459, 515, 774
Hpy99I	CGWCG	1 cut(s)	542
HpyAV	CCTTC	3 cut(s)	461, 1294, 1863
HpyCH4III	ACNGT	4 cut(s)	778, 784, 1450, 1507
HpyCH4IV	ACGT	5 cut(s)	54, 980, 1398, 1627, 2052
HpyF10VI	GCNNNNNNNGC	4 cut(s)	623, 761, 1155, 1622
HpySE526I	ACGT	5 cut(s)	54, 980, 1398, 1627, 2052
Hsp92I	GRCGYC	1 cut(s)	2052
Hsp92II	CATG	8 cut(s)	64, 292, 955, 1367, 1427, 1552, 1954, 2045
HspAI	GCGC	1 cut(s)	1158
Kzo9I	GATC	8 cut(s)	327, 1323, 1341, 1437, 1600, 1852, 1941, 2033
LmnI	GCTCC	4 cut(s)	103, 374, 1351, 1930
Lsp1109I	GCAGC	7 cut(s)	626, 775, 785, 1473, 1561, 1634, 1986
LweI	GCATC	4 cut(s)	1266, 1350, 1720, 1813
MaeI	CTAG	8 cut(s)	149, 524, 1121, 1184, 1208, 1382, 1517, 1719
MaeII	ACGT	5 cut(s)	54, 980, 1398, 1627, 2052
MaeIII	GTNAC	7 cut(s)	778, 969, 1450, 1543, 1654, 1688, 2048
MalI	GATC	8 cut(s)	329, 1325, 1343, 1439, 1602, 1854, 1943, 2035
MboI	GATC	8 cut(s)	327, 1323, 1341, 1437, 1600, 1852, 1941, 2033
MflI	RGATCY	1 cut(s)	1437
MhlI	GDGCHC	4 cut(s)	100, 517, 991, 1151
MlyI	GAGTC	1 cut(s)	2032
MmeI	TCCRAC	4 cut(s)	561, 1489, 1704, 1846
Mph1103I	ATGCAT	1 cut(s)	1365
MroXI	GAANNNNTTC	1 cut(s)	654
MseI	TTAA	6 cut(s)	35, 453, 1029, 1253, 1749, 1779
MslI	CAYNNNNRTG	1 cut(s)	2046
MspI	CCGG	2 cut(s)	1536, 1541
MspR9I	CCNGG	7 cut(s)	102, 165, 174, 228, 1435, 1536, 1685
Mva1269I	GAATGC	1 cut(s)	174
MvaI	CCWGG	6 cut(s)	102, 165, 174, 228, 1435, 1685
MvnI	CGCG	1 cut(s)	1605
MwoI	GCNNNNNNNGC	4 cut(s)	623, 761, 1155, 1622
NciI	CCSGG	1 cut(s)	1536
NdeII	GATC	8 cut(s)	327, 1323, 1341, 1437, 1600, 1852, 1941, 2033
NlaIII	CATG	8 cut(s)	64, 292, 955, 1367, 1427, 1552, 1954, 2045
NlaIV	GGNNCC	2 cut(s)	751, 1532
NmuCI	GTSAC	5 cut(s)	778, 1450, 1543, 1654, 2048
NruI	TCGCGA	1 cut(s)	1605
NsiI	ATGCAT	1 cut(s)	1365
NspI	RCATGY	1 cut(s)	1954
PagI	TCATGA	1 cut(s)	1423
PceI	AGGCCT	2 cut(s)	135, 242
PctI	GAATGC	1 cut(s)	174
PdmI	GAANNNNTTC	1 cut(s)	654
PfeI	GAWTC	4 cut(s)	141, 1328, 1669, 1835
PflFI	GACNNNGTC	1 cut(s)	1549
PflMI	CCANNNNNTGG	1 cut(s)	1648
PfoI	TCCNGGA	1 cut(s)	163
PkrI	GCNGC	9 cut(s)	616, 754, 790, 800, 1488, 1576, 1624, 1875, 2001
PleI	GAGTC	1 cut(s)	2032
PpsI	GAGTC	1 cut(s)	2032
Ppu21I	YACGTR	3 cut(s)	981, 1399, 1628
PpuMI	RGGWCCY	3 cut(s)	566, 1019, 1313
Psp5II	RGGWCCY	3 cut(s)	566, 1019, 1313
Psp6I	CCWGG	6 cut(s)	100, 163, 172, 226, 1433, 1683
PspEI	GGTNACC	1 cut(s)	1543
PspFI	CCCAGC	1 cut(s)	1933
PspGI	CCWGG	6 cut(s)	100, 163, 172, 226, 1433, 1683
PspN4I	GGNNCC	2 cut(s)	751, 1532
PspPI	GGNCC	8 cut(s)	277, 566, 599, 1004, 1019, 1313, 1538, 1592
PspPPI	RGGWCCY	3 cut(s)	566, 1019, 1313
PsuI	RGATCY	1 cut(s)	1437
PsyI	GACNNNGTC	1 cut(s)	1549
RruI	TCGCGA	1 cut(s)	1605
RsaI	GTAC	3 cut(s)	983, 1401, 2011
RsaNI	GTAC	3 cut(s)	982, 1400, 2010
RseI	CAYNNNNRTG	1 cut(s)	2046
Rsr2I	CGGWCCG	2 cut(s)	599, 1538
RsrII	CGGWCCG	2 cut(s)	599, 1538
SaqAI	TTAA	6 cut(s)	35, 453, 1029, 1253, 1749, 1779
SatI	GCNGC	9 cut(s)	615, 753, 789, 799, 1487, 1575, 1623, 1874, 2000
Sau3AI	GATC	8 cut(s)	327, 1323, 1341, 1437, 1600, 1852, 1941, 2033
Sau96I	GGNCC	8 cut(s)	277, 566, 599, 1004, 1019, 1313, 1538, 1592
SchI	GAGTC	1 cut(s)	2032
ScrFI	CCNGG	7 cut(s)	102, 165, 174, 228, 1435, 1536, 1685
SduI	GDGCHC	4 cut(s)	100, 517, 991, 1151
SfaNI	GCATC	4 cut(s)	1266, 1350, 1720, 1813
SfcI	CTRYAG	1 cut(s)	1503
SinI	GGWCC	6 cut(s)	566, 599, 1019, 1313, 1538, 1592
SmiMI	CAYNNNNRTG	1 cut(s)	2046
SmlI	CTYRAG	1 cut(s)	1306
SmoI	CTYRAG	1 cut(s)	1306
SpeI	ACTAGT	1 cut(s)	1516
SseBI	AGGCCT	2 cut(s)	135, 242
SsiI	CCGC	8 cut(s)	122, 434, 602, 753, 1102, 1590, 1873, 1928
SspMI	CTAG	8 cut(s)	149, 524, 1121, 1184, 1208, 1382, 1517, 1719
StuI	AGGCCT	2 cut(s)	135, 242
StyD4I	CCNGG	7 cut(s)	100, 163, 172, 226, 1433, 1534, 1683
TaaI	ACNGT	4 cut(s)	778, 784, 1450, 1507
TaiI	ACGT	5 cut(s)	57, 983, 1401, 1630, 2055
TaqI	TCGA	7 cut(s)	880, 1065, 1179, 1326, 1521, 1794, 2036
TauI	GCSGC	2 cut(s)	755, 1876
TfiI	GAWTC	4 cut(s)	141, 1328, 1669, 1835
Tru1I	TTAA	6 cut(s)	35, 453, 1029, 1253, 1749, 1779
Tru9I	TTAA	6 cut(s)	35, 453, 1029, 1253, 1749, 1779
TscAI	CASTG	2 cut(s)	898, 1813
TseFI	GTSAC	5 cut(s)	778, 1450, 1543, 1654, 2048
TseI	GCWGC	7 cut(s)	614, 788, 798, 1486, 1574, 1622, 1999
Tsp45I	GTSAC	5 cut(s)	778, 1450, 1543, 1654, 2048
TspGWI	ACGGA	3 cut(s)	387, 612, 2027
TspRI	CASTG	2 cut(s)	898, 1813
Tth111I	GACNNNGTC	1 cut(s)	1549
Van91I	CCANNNNNTGG	1 cut(s)	1648
VneI	GTGCAC	1 cut(s)	513
VpaK11BI	GGWCC	6 cut(s)	566, 599, 1019, 1313, 1538, 1592
XapI	RAATTY	4 cut(s)	180, 635, 1255, 2062
XceI	RCATGY	1 cut(s)	1954
XmnI	GAANNNNTTC	1 cut(s)	654
XspI	CTAG	8 cut(s)	149, 524, 1121, 1184, 1208, 1382, 1517, 1719
ZraI	GACGTC	1 cut(s)	2053
Zsp2I	ATGCAT	1 cut(s)	1365

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.