Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0001152

Physical Location & Seq

Genomic Coordinates Forward (+)

26546774 .. 26549743

Gene Structure 2970 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1158800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1989 bp

ATGGTTAGGACACAAGAGAATGCCTGGAAGATTGGTCTAGCAGGCTTCCTTTTTCCAAGCGCTGTTACCTCAAGCCTCGTCTACCCGCTAGCCCGATCCATTCCAGGACTCACAGGAGGGATACTCTTTCTTTTCTTTACTACATTTTCTTTATCCTTCACTTATTTTCCTACCGTGGCTGAGGCCTTGGATGAACTCAACCTCATGACTTCCGAGTTAGGGCAACTCGCCTTGTCTTCTGCCATGCTCAACGATGTGATGCAATGGTTCTTCATGGCAATACGTTACATATACAGCCACGGAATCGAGGCCTTGGTTTCCTTTAAAATACCCAAAGGAAAAGAAGTGAGTGAAAGTTATGTGGTTGCAATACAACTTGGAGTTCTAGTCATGGCATTTATCACCGACGCCTTAGGCACAAAGCCTACTACGGGTGCTATAATTCTAGGGTTAGTCATACCAAATCGACCACCTCTTGGTACAGCATTGATAAAAAAGACAGAATTCATGGTCTCCGAATTTCTTCTCCCGGTGTTCTTCTTTTGTGCAGGCCTCAGTGTGAATGTGTATTCGATTCACGATTGGGAAAGTTTCAGCAAACTTCAAGTCTTGATTGTTGAATCCTATGTCACAAAATTTTTGGGAGTTACATTGGCTGCAATGTGCTGCAAAATTAAGTTCAAACATAGTCTGTTGCTCAGCATGATCATGAGCATCAAGGGTTTAATAGAACCTTCCATTTACAGTCGCTGGAGGGTCCTTAAGCAAATGGATCAACAATCTTATACTCAAATCATCTTGTCTTCGCTGGGTCTGACCATGGCCATAGCACCATTTATACGATTCTCGTGCATACTTCATGTACACCTCAGACAATCAAGTAGCATACACGACTGCATTAGAAATATTCAATCATTACTGGCAAGCAACTCGGAAACATTTCAAATCCTTCGCTGTGTCCACAACGAAGAAAGTGTACGCAACATGATCACCCTCCTAGAGGCGTCAAACCCAACAGAAGCAAGCCCGATTTGTGCCTACATTTTCCATGCAGTCGAGCTCACTGTCAGCGCAGCACCACTACTAGTCCGTCACACGAAACAGAAGAAGAAATTTACAAACACAAGTGTACCCACGAATGTTCATATAATGCGAGCCTTTGAGAATTACTCGAAAAATTCGAAAGGGCCGGTTGTAATTGAAGCCTTCACTATGACCGCCCCCTATATAAGCATGCATGAGACGATTTGCCATGAAGCCAATGACAAGAAAGTTCCTCTAATTATCATACCATTCCATGAAAACCACAAGGATGTGATTGCTCCCAACTGTCCGACTAAGGCAATACGCCAGTTCAACATTAACGTACAATCATATGCTCCATGTACAGTTGGGATACTAGTTGATAGAAGCCTGTCTTGTCGGCTGAGCTTGAATCACTTCTCTTACAATGTTGCGGTGTTTTTCATTGGCGGGCCTGATGATCGCGAGGCATTAGCTTACTCAGCACGCATGCCGGGTAATACCAAAGTTTCCATCTCGGTGTTCAGGTTTACTTTGGCGGAGACAGTAATAGAAGAAGAGCAGCTGAAAGAAACGAAGCTCGACCAGGCTTTGGTTGACGAGTTTAAGCTAAGCAACATTGGGAATGATCACTTGAATTGGCATGAAATGGAGGTGGCTGACGGCGTGCAGCTTATGGATGCCATTAGGCAGTCACATGGCGATTACGACCTTGTGATGGTGGGACGGAGACACTTGGAAATGTCACCAAGGAATGAAGAAACGGTAGATTTTATGGAGAACGCGGAGCTGGGAGCGATCGGCGACATGCTTGCTTCCTCAGATTTTTGTGGTGGAATGGTAAATGTGTTGGTGATGCAAGAAAGTAGGGAATTCGTACTTCGAACCGCTTTGATGAAAAATTTAACGAGGTATTATAAGTTGACATACATTGCAAAACCAGCTTGCAGGGGACAAACTAGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0042592 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

663

Amino Acids

74.09

Weight (kDa)

6.83

Isoelectric Point (pI)

42.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	8 - 94	1.6e-06	Sodium/hydrogen exchanger, transmembrane
Na_H_Exchanger	PF00999	114 - 270	2.3e-14	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	346 - 473	8.3e-07	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	483 - 629	5.7e-08	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001152)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g43690 FvH4_7g25211 FvH4_7g25250 FvH4_7g25290 FvH4_7g25310
malus_domestica	MD01G1158700.v1.1 MD01G1158800.v1.1 MD07G1227200.v1.1 MD07G1227600.v1.1
prunus_persica	Prupe.2G257000_v2.0.a1 Prupe.2G257100_v2.0.a1
pyrus_communis	pycom01g17540
rosa_chinensis	RchiOBHm_Chr1g0371191 RchiOBHm_Chr1g0371201 RchiOBHm_Chr1g0371211
rosa_laevigata	RLG00000026978 RLG00000026979 RLG00000026980 RLG00000026981 RLG00000026983 RLG00000036771
rosa_multiflora	Rmu_co8222838.1_g000001 Rmu_sc0000631.1_g000023 Rmu_sc0001018.1_g000042 Rmu_sc0001018.1_g000044 Rmu_sc0001018.1_g000049 Rmu_sc0001018.1_g000050 Rmu_sc0001018.1_g000051 Rmu_sc0008081.1_g000003
rosa_roxburghii	Rroxscaffold_4G00285900 Rroxscaffold_4G00285910
rosa_rugosa	Rorug01G0361700 Rorug01G0361900 Rorug01G0362000 Rorug01G0362200 Rorug05G0460600
rosa_samantha	Rh1AG372000 Rh1AG372200 Rh1AG372300 Rh1BG335100 Rh1BG335500 Rh1BG335700 Rh1BG335800 Rh1CG348600 Rh1CG349000 Rh1CG349100 Rh1CG349200 Rh1DG366500 Rh1DG366900 Rh1DG367000 Rh5BG537300 Rh5CG560800 Rh5DG548900
rosa_wichuraiana	Rw1G032720 Rw1G032730 Rw1G032740 Rw5G047750 Rw5G047880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1941
AccB7I	CCANNNNNTGG	2 cut(s)	476, 1615
AccI	GTMKAC	1 cut(s)	81
AccII	CGCG	2 cut(s)	1488, 1808
AciI	CCGC	7 cut(s)	86, 1218, 1457, 1473, 1562, 1808, 1911
AclWI	GGATC	2 cut(s)	90, 780
AcoI	YGGCCR	1 cut(s)	822
AcsI	RAATTY	7 cut(s)	503, 518, 635, 1112, 1177, 1895, 1924
AcyI	GRCGYC	2 cut(s)	408, 1004
AfaI	GTAC	7 cut(s)	481, 864, 978, 1131, 1368, 1387, 1902
AfeI	AGCGCT	1 cut(s)	61
AfiI	CCNNNNNNNGG	6 cut(s)	219, 431, 476, 1000, 1615, 1741
AflII	CTTAAG	1 cut(s)	761
AgsI	TTSAA	9 cut(s)	605, 620, 682, 911, 944, 1202, 1357, 1435, 1660
AhlI	ACTAGT	2 cut(s)	1084, 1399
AjnI	CCWGG	3 cut(s)	23, 103, 1608
Alw21I	GWGCWC	1 cut(s)	1062
Alw26I	GTCTC	4 cut(s)	517, 1235, 1559, 1747
AlwI	GGATC	2 cut(s)	90, 780
AlwNI	CAGNNNCTG	1 cut(s)	750
Aor51HI	AGCGCT	1 cut(s)	61
AoxI	GGCC	6 cut(s)	183, 309, 550, 822, 1187, 1475
ApeKI	GCWGC	5 cut(s)	656, 666, 1073, 1585, 1693
ApoI	RAATTY	7 cut(s)	503, 518, 635, 1112, 1177, 1895, 1924
ArsI	GACNNNNNNTTYG	2 cut(s)	591, 623
Asp700I	GAANNNNTTC	3 cut(s)	522, 939, 1439
AspLEI	GCGC	2 cut(s)	62, 1073
AspS9I	GGNCC	3 cut(s)	757, 1187, 1475
AsuC2I	CCSGG	2 cut(s)	530, 1518
AsuHPI	GGTGA	4 cut(s)	394, 982, 1761, 1888
AsuII	TTCGAA	2 cut(s)	1181, 1906
AsuNHI	GCTAGC	1 cut(s)	88
AvaII	GGWCC	1 cut(s)	757
AxyI	CCTNAGG	1 cut(s)	412
BaeI	ACNNNNGTAYC	2 cut(s)	1113, 1146
BalI	TGGCCA	1 cut(s)	824
BanII	GRGCYC	1 cut(s)	1062
BarI	GAAGNNNNNNTAC	2 cut(s)	960, 992
BauI	CACGAG	1 cut(s)	847
BbsI	GAAGAC	2 cut(s)	228, 795
Bbv12I	GWGCWC	1 cut(s)	1062
BbvCI	CCTCAGC	1 cut(s)	180
BbvI	GCAGC	5 cut(s)	643, 653, 1085, 1597, 1705
BccI	CCATC	2 cut(s)	1544, 1735
BceAI	ACGGC	1 cut(s)	1702
BcgI	CGANNNNNNTGC	6 cut(s)	831, 865, 912, 946, 1817, 1851
BciT130I	CCWGG	3 cut(s)	25, 105, 1610
BciVI	GTATCC	2 cut(s)	114, 1389
BclI	TGATCA	3 cut(s)	705, 987, 1651
BcnI	CCSGG	2 cut(s)	530, 1518
BcoDI	GTCTC	4 cut(s)	517, 1235, 1559, 1747
BcuI	ACTAGT	2 cut(s)	1084, 1399
BfaI	CTAG	9 cut(s)	38, 89, 386, 446, 998, 1085, 1400, 1983, 1987
BfoI	RGCGCY	1 cut(s)	63
BfrI	CTTAAG	1 cut(s)	761
BfuI	GTATCC	2 cut(s)	114, 1389
BisI	GCNGC	5 cut(s)	657, 667, 1074, 1586, 1694
BlpI	GCTNAGC	3 cut(s)	698, 1427, 1634
BlsI	GCNGC	5 cut(s)	658, 668, 1075, 1587, 1695
Bme1390I	CCNGG	5 cut(s)	25, 105, 530, 1518, 1610
Bme18I	GGWCC	1 cut(s)	757
BmgT120I	GGNCC	3 cut(s)	757, 1187, 1475
BmiI	GGNNCC	1 cut(s)	758
BmrFI	CCNGG	5 cut(s)	25, 105, 530, 1518, 1610
BmsI	GCATC	4 cut(s)	249, 723, 1693, 1869
BmtI	GCTAGC	1 cut(s)	92
BpiI	GAAGAC	2 cut(s)	228, 795
BplI	GAGNNNNNCTC	4 cut(s)	108, 140, 1154, 1186
BpmI	CTGGAG	1 cut(s)	772
Bpu10I	CCTNAGC	1 cut(s)	180
Bpu1102I	GCTNAGC	3 cut(s)	698, 1427, 1634
Bpu14I	TTCGAA	2 cut(s)	1181, 1906
BpuEI	CTTGAG	1 cut(s)	55
BpuMI	CCSGG	2 cut(s)	530, 1518
BsaHI	GRCGYC	2 cut(s)	408, 1004
BsaI	GGTCTC	1 cut(s)	517
BsaJI	CCNNGG	6 cut(s)	174, 186, 298, 312, 819, 1772
BsaXI	ACNNNNNCTCC	2 cut(s)	372, 402
Bsc4I	CCNNNNNNNGG	6 cut(s)	219, 431, 476, 1000, 1615, 1741
Bse118I	RCCGGY	1 cut(s)	1189
Bse1I	ACTGG	2 cut(s)	924, 1351
Bse21I	CCTNAGG	1 cut(s)	412
Bse3DI	GCAATG	3 cut(s)	269, 666, 1953
BseBI	CCWGG	3 cut(s)	25, 105, 1610
BseDI	CCNNGG	6 cut(s)	174, 186, 298, 312, 819, 1772
BseGI	GGATG	3 cut(s)	196, 1318, 1708
BseLI	CCNNNNNNNGG	6 cut(s)	219, 431, 476, 1000, 1615, 1741
BseMI	GCAATG	3 cut(s)	269, 666, 1953
BseMII	CTCAG	7 cut(s)	171, 568, 712, 883, 1418, 1518, 1857
BseNI	ACTGG	2 cut(s)	924, 1351
BseXI	GCAGC	5 cut(s)	643, 653, 1085, 1597, 1705
BseYI	CCCAGC	2 cut(s)	808, 1813
BsgI	GTGCAG	2 cut(s)	567, 1712
Bsh1236I	CGCG	2 cut(s)	1488, 1808
Bsh1285I	CGRYCG	1 cut(s)	1824
BshFI	GGCC	6 cut(s)	185, 311, 552, 824, 1189, 1477
BsiEI	CGRYCG	1 cut(s)	1824
BsiHKAI	GWGCWC	1 cut(s)	1062
BsiSI	CCGG	3 cut(s)	530, 1190, 1517
BslFI	GGGAC	1 cut(s)	1761
BslI	CCNNNNNNNGG	6 cut(s)	219, 431, 476, 1000, 1615, 1741
BsmAI	GTCTC	4 cut(s)	517, 1235, 1559, 1747
BsmBI	CGTCTC	1 cut(s)	1235
BsmFI	GGGAC	1 cut(s)	1761
BsmI	GAATGC	1 cut(s)	25
BsnI	GGCC	6 cut(s)	185, 311, 552, 824, 1189, 1477
Bso31I	GGTCTC	1 cut(s)	517
Bsp119I	TTCGAA	2 cut(s)	1181, 1906
Bsp1286I	GDGCHC	1 cut(s)	1062
Bsp1407I	TGTACA	2 cut(s)	862, 1385
Bsp143I	GATC	7 cut(s)	95, 705, 772, 987, 1483, 1651, 1821
Bsp1720I	GCTNAGC	3 cut(s)	698, 1427, 1634
Bsp19I	CCATGG	1 cut(s)	819
Bsp68I	TCGCGA	1 cut(s)	1488
BspACI	CCGC	7 cut(s)	86, 1218, 1457, 1473, 1562, 1808, 1911
BspANI	GGCC	6 cut(s)	185, 311, 552, 824, 1189, 1477
BspCNI	CTCAG	7 cut(s)	172, 567, 711, 882, 1419, 1517, 1856
BspFNI	CGCG	2 cut(s)	1488, 1808
BspHI	TCATGA	2 cut(s)	204, 708
BspLI	GGNNCC	1 cut(s)	758
BspOI	GCTAGC	1 cut(s)	92
BspPI	GGATC	2 cut(s)	90, 780
BspQI	GCTCTTC	1 cut(s)	1575
BspT104I	TTCGAA	2 cut(s)	1181, 1906
BspTI	CTTAAG	1 cut(s)	761
BspTNI	GGTCTC	1 cut(s)	517
BsrDI	GCAATG	3 cut(s)	269, 666, 1953
BsrFI	RCCGGY	1 cut(s)	1189
BsrGI	TGTACA	2 cut(s)	862, 1385
BsrI	ACTGG	2 cut(s)	924, 1351
BssAI	RCCGGY	1 cut(s)	1189
BssECI	CCNNGG	6 cut(s)	174, 186, 298, 312, 819, 1772
BssMI	GATC	7 cut(s)	95, 705, 772, 987, 1483, 1651, 1821
BssNI	GRCGYC	2 cut(s)	408, 1004
BssSI	CACGAG	1 cut(s)	847
BssT1I	CCWWGG	4 cut(s)	186, 312, 819, 1772
Bst2BI	CACGAG	1 cut(s)	847
Bst2UI	CCWGG	3 cut(s)	25, 105, 1610
Bst4CI	ACNGT	7 cut(s)	175, 746, 1066, 1331, 1390, 1570, 1789
Bst6I	CTCTTC	1 cut(s)	1575
BstACI	GRCGYC	2 cut(s)	408, 1004
BstAFI	CTTAAG	1 cut(s)	761
BstAUI	TGTACA	2 cut(s)	862, 1385
BstBI	TTCGAA	2 cut(s)	1181, 1906
BstDSI	CCRYGG	3 cut(s)	174, 298, 819
BstENI	CCTNNNNNAGG	1 cut(s)	998
BstF5I	GGATG	3 cut(s)	196, 1318, 1708
BstFNI	CGCG	2 cut(s)	1488, 1808
BstH2I	RGCGCY	1 cut(s)	63
BstHHI	GCGC	2 cut(s)	62, 1073
BstKTI	GATC	7 cut(s)	98, 708, 775, 990, 1486, 1654, 1824
BstMAI	GTCTC	4 cut(s)	517, 1235, 1559, 1747
BstMBI	GATC	7 cut(s)	95, 705, 772, 987, 1483, 1651, 1821
BstMCI	CGRYCG	1 cut(s)	1824
BstMWI	GCNNNNNNNGC	2 cut(s)	1505, 1964
BstNI	CCWGG	3 cut(s)	25, 105, 1610
BstNSI	RCATGY	3 cut(s)	1237, 1516, 1834
BstSCI	CCNGG	5 cut(s)	23, 103, 528, 1516, 1608
BstUI	CGCG	2 cut(s)	1488, 1808
BstV1I	GCAGC	5 cut(s)	643, 653, 1085, 1597, 1705
BstV2I	GAAGAC	2 cut(s)	228, 795
Bsu36I	CCTNAGG	1 cut(s)	412
BsuI	GTATCC	2 cut(s)	114, 1389
BsuRI	GGCC	6 cut(s)	185, 311, 552, 824, 1189, 1477
BtgI	CCRYGG	3 cut(s)	174, 298, 819
BtsCI	GGATG	3 cut(s)	196, 1318, 1708
BtsIMutI	CAGTG	2 cut(s)	562, 1062
BtuMI	TCGCGA	1 cut(s)	1488
CaiI	CAGNNNCTG	1 cut(s)	750
CciI	TCATGA	2 cut(s)	204, 708
CfoI	GCGC	2 cut(s)	62, 1073
Cfr10I	RCCGGY	1 cut(s)	1189
Cfr13I	GGNCC	3 cut(s)	757, 1187, 1475
CseI	GACGC	2 cut(s)	416, 993
Csp6I	GTAC	7 cut(s)	480, 863, 977, 1130, 1367, 1386, 1901
CviQI	GTAC	7 cut(s)	480, 863, 977, 1130, 1367, 1386, 1901
DpnI	GATC	7 cut(s)	97, 707, 774, 989, 1485, 1653, 1823
DpnII	GATC	7 cut(s)	95, 705, 772, 987, 1483, 1651, 1821
DraI	TTTAAA	1 cut(s)	325
EaeI	YGGCCR	1 cut(s)	822
Eam1104I	CTCTTC	1 cut(s)	1575
EarI	CTCTTC	1 cut(s)	1575
EciI	GGCGGA	1 cut(s)	1577
Ecl136II	GAGCTC	1 cut(s)	1060
Eco130I	CCWWGG	4 cut(s)	186, 312, 819, 1772
Eco147I	AGGCCT	3 cut(s)	185, 311, 552
Eco24I	GRGCYC	1 cut(s)	1062
Eco31I	GGTCTC	1 cut(s)	517
Eco47I	GGWCC	1 cut(s)	757
Eco47III	AGCGCT	1 cut(s)	61
Eco53kI	GAGCTC	1 cut(s)	1060
Eco81I	CCTNAGG	1 cut(s)	412
EcoICRI	GAGCTC	1 cut(s)	1060
EcoNI	CCTNNNNNAGG	1 cut(s)	998
EcoO109I	RGGNCCY	1 cut(s)	757
EcoRI	GAATTC	2 cut(s)	503, 1895
EcoRII	CCWGG	3 cut(s)	23, 103, 1608
EcoT14I	CCWWGG	4 cut(s)	186, 312, 819, 1772
EcoT22I	ATGCAT	1 cut(s)	1239
EcoT38I	GRGCYC	1 cut(s)	1062
ErhI	CCWWGG	4 cut(s)	186, 312, 819, 1772
Esp3I	CGTCTC	1 cut(s)	1235
FalI	AAGNNNNNCTT	2 cut(s)	1402, 1434
FaqI	GGGAC	1 cut(s)	1761
FauI	CCCGC	2 cut(s)	93, 1466
FauNDI	CATATG	1 cut(s)	1375
FbaI	TGATCA	3 cut(s)	705, 987, 1651
FblI	GTMKAC	1 cut(s)	81
Fnu4HI	GCNGC	5 cut(s)	657, 667, 1074, 1586, 1694
FokI	GGATG	3 cut(s)	203, 1325, 1715
FriOI	GRGCYC	1 cut(s)	1062
Fsp4HI	GCNGC	5 cut(s)	657, 667, 1074, 1586, 1694
FspBI	CTAG	9 cut(s)	38, 89, 386, 446, 998, 1085, 1400, 1983, 1987
GlaI	GCGC	2 cut(s)	61, 1072
GluI	GCNGC	5 cut(s)	657, 667, 1074, 1586, 1694
GsaI	CCCAGC	2 cut(s)	812, 1817
GsuI	CTGGAG	1 cut(s)	772
HaeII	RGCGCY	1 cut(s)	63
HaeIII	GGCC	6 cut(s)	185, 311, 552, 824, 1189, 1477
HapII	CCGG	3 cut(s)	530, 1190, 1517
HgaI	GACGC	2 cut(s)	416, 993
HhaI	GCGC	2 cut(s)	62, 1073
Hin1I	GRCGYC	2 cut(s)	408, 1004
Hin6I	GCGC	2 cut(s)	60, 1071
HinP1I	GCGC	2 cut(s)	60, 1071
HincII	GTYRAC	2 cut(s)	1621, 1947
HindII	GTYRAC	2 cut(s)	1621, 1947
HinfI	GANTC	6 cut(s)	108, 303, 574, 620, 843, 1435
HpaII	CCGG	3 cut(s)	530, 1190, 1517
HphI	GGTGA	4 cut(s)	394, 982, 1761, 1888
Hpy166II	GTNNAC	8 cut(s)	82, 865, 961, 977, 1130, 1554, 1621, 1947
Hpy188I	TCNGA	7 cut(s)	214, 517, 816, 872, 934, 1335, 1846
Hpy188III	TCNNGA	5 cut(s)	205, 578, 610, 709, 1487
Hpy8I	GTNNAC	8 cut(s)	82, 865, 961, 977, 1130, 1554, 1621, 1947
Hpy99I	CGWCG	1 cut(s)	410
HpyAV	CCTTC	4 cut(s)	166, 744, 959, 1216
HpyCH4III	ACNGT	7 cut(s)	175, 746, 1066, 1331, 1390, 1570, 1789
HpyCH4IV	ACGT	2 cut(s)	283, 1365
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1505, 1964
HpySE526I	ACGT	2 cut(s)	283, 1365
Hsp92I	GRCGYC	2 cut(s)	408, 1004
HspAI	GCGC	2 cut(s)	60, 1071
Ksp22I	TGATCA	3 cut(s)	705, 987, 1651
Kzo9I	GATC	7 cut(s)	95, 705, 772, 987, 1483, 1651, 1821
LguI	GCTCTTC	1 cut(s)	1575
LmnI	GCTCC	4 cut(s)	1327, 1384, 1810, 1817
Lsp1109I	GCAGC	5 cut(s)	643, 653, 1085, 1597, 1705
LweI	GCATC	4 cut(s)	249, 723, 1693, 1869
MaeI	CTAG	9 cut(s)	38, 89, 386, 446, 998, 1085, 1400, 1983, 1987
MaeII	ACGT	2 cut(s)	283, 1365
MaeIII	GTNAC	7 cut(s)	64, 284, 628, 646, 1091, 1716, 1767
MalI	GATC	7 cut(s)	97, 707, 774, 989, 1485, 1653, 1823
MboI	GATC	7 cut(s)	95, 705, 772, 987, 1483, 1651, 1821
MhlI	GDGCHC	1 cut(s)	1062
MlsI	TGGCCA	1 cut(s)	824
MluNI	TGGCCA	1 cut(s)	824
MlyI	GAGTC	1 cut(s)	102
MmeI	TCCRAC	1 cut(s)	1358
Mox20I	TGGCCA	1 cut(s)	824
Mph1103I	ATGCAT	1 cut(s)	1239
MroXI	GAANNNNTTC	3 cut(s)	522, 939, 1439
MscI	TGGCCA	1 cut(s)	824
MseI	TTAA	7 cut(s)	324, 675, 725, 762, 1362, 1629, 1928
MslI	CAYNNNNRTG	3 cut(s)	707, 1311, 1541
Msp20I	TGGCCA	1 cut(s)	824
MspA1I	CMGCKG	1 cut(s)	1588
MspCI	CTTAAG	1 cut(s)	761
MspI	CCGG	3 cut(s)	530, 1190, 1517
MspR9I	CCNGG	5 cut(s)	25, 105, 530, 1518, 1610
Mva1269I	GAATGC	1 cut(s)	25
MvaI	CCWGG	3 cut(s)	25, 105, 1610
MvnI	CGCG	2 cut(s)	1488, 1808
MwoI	GCNNNNNNNGC	2 cut(s)	1505, 1964
NciI	CCSGG	2 cut(s)	530, 1518
NcoI	CCATGG	1 cut(s)	819
NdeI	CATATG	1 cut(s)	1375
NdeII	GATC	7 cut(s)	95, 705, 772, 987, 1483, 1651, 1821
NheI	GCTAGC	1 cut(s)	88
NlaIV	GGNNCC	1 cut(s)	758
NmuCI	GTSAC	4 cut(s)	628, 1091, 1716, 1767
NruI	TCGCGA	1 cut(s)	1488
NsiI	ATGCAT	1 cut(s)	1239
NspI	RCATGY	3 cut(s)	1237, 1516, 1834
NspV	TTCGAA	2 cut(s)	1181, 1906
PaeI	GCATGC	2 cut(s)	1237, 1516
PagI	TCATGA	2 cut(s)	204, 708
PceI	AGGCCT	3 cut(s)	185, 311, 552
PciSI	GCTCTTC	1 cut(s)	1575
PcsI	WCGNNNNNNNCGW	1 cut(s)	1178
PctI	GAATGC	1 cut(s)	25
PdmI	GAANNNNTTC	3 cut(s)	522, 939, 1439
PfeI	GAWTC	5 cut(s)	303, 574, 620, 843, 1435
PflMI	CCANNNNNTGG	2 cut(s)	476, 1615
PfoI	TCCNGGA	1 cut(s)	103
PkrI	GCNGC	5 cut(s)	658, 668, 1075, 1587, 1695
Ple19I	CGATCG	1 cut(s)	1824
PleI	GAGTC	1 cut(s)	102
PpsI	GAGTC	1 cut(s)	102
PpuMI	RGGWCCY	1 cut(s)	757
PsiI	TTATAA	1 cut(s)	1941
Psp124BI	GAGCTC	1 cut(s)	1062
Psp5II	RGGWCCY	1 cut(s)	757
Psp6I	CCWGG	3 cut(s)	23, 103, 1608
PspFI	CCCAGC	2 cut(s)	808, 1813
PspGI	CCWGG	3 cut(s)	23, 103, 1608
PspN4I	GGNNCC	1 cut(s)	758
PspPI	GGNCC	3 cut(s)	757, 1187, 1475
PspPPI	RGGWCCY	1 cut(s)	757
PstNI	CAGNNNCTG	1 cut(s)	750
PvuI	CGATCG	1 cut(s)	1824
PvuII	CAGCTG	1 cut(s)	1588
RruI	TCGCGA	1 cut(s)	1488
RsaI	GTAC	7 cut(s)	481, 864, 978, 1131, 1368, 1387, 1902
RsaNI	GTAC	7 cut(s)	480, 863, 977, 1130, 1367, 1386, 1901
RseI	CAYNNNNRTG	3 cut(s)	707, 1311, 1541
SacI	GAGCTC	1 cut(s)	1062
SapI	GCTCTTC	1 cut(s)	1575
SaqAI	TTAA	7 cut(s)	324, 675, 725, 762, 1362, 1629, 1928
SatI	GCNGC	5 cut(s)	657, 667, 1074, 1586, 1694
Sau3AI	GATC	7 cut(s)	95, 705, 772, 987, 1483, 1651, 1821
Sau96I	GGNCC	3 cut(s)	757, 1187, 1475
SchI	GAGTC	1 cut(s)	102
ScrFI	CCNGG	5 cut(s)	25, 105, 530, 1518, 1610
SduI	GDGCHC	1 cut(s)	1062
SfaNI	GCATC	4 cut(s)	249, 723, 1693, 1869
SfuI	TTCGAA	2 cut(s)	1181, 1906
SinI	GGWCC	1 cut(s)	757
SmiMI	CAYNNNNRTG	3 cut(s)	707, 1311, 1541
SmlI	CTYRAG	2 cut(s)	70, 761
SmoI	CTYRAG	2 cut(s)	70, 761
SpeI	ACTAGT	2 cut(s)	1084, 1399
SphI	GCATGC	2 cut(s)	1237, 1516
SseBI	AGGCCT	3 cut(s)	185, 311, 552
SsiI	CCGC	7 cut(s)	86, 1218, 1457, 1473, 1562, 1808, 1911
SspI	AATATT	1 cut(s)	907
SspMI	CTAG	9 cut(s)	38, 89, 386, 446, 998, 1085, 1400, 1983, 1987
SstI	GAGCTC	1 cut(s)	1062
StuI	AGGCCT	3 cut(s)	185, 311, 552
StyD4I	CCNGG	5 cut(s)	23, 103, 528, 1516, 1608
StyI	CCWWGG	4 cut(s)	186, 312, 819, 1772
TaaI	ACNGT	7 cut(s)	175, 746, 1066, 1331, 1390, 1570, 1789
TaiI	ACGT	2 cut(s)	286, 1368
TaqI	TCGA	8 cut(s)	306, 466, 572, 1056, 1172, 1181, 1605, 1906
TatI	WGTACW	2 cut(s)	862, 1385
TfiI	GAWTC	5 cut(s)	303, 574, 620, 843, 1435
Tru1I	TTAA	7 cut(s)	324, 675, 725, 762, 1362, 1629, 1928
Tru9I	TTAA	7 cut(s)	324, 675, 725, 762, 1362, 1629, 1928
TscAI	CASTG	2 cut(s)	562, 1069
TseFI	GTSAC	4 cut(s)	628, 1091, 1716, 1767
TseI	GCWGC	5 cut(s)	656, 666, 1073, 1585, 1693
Tsp45I	GTSAC	4 cut(s)	628, 1091, 1716, 1767
TspGWI	ACGGA	3 cut(s)	315, 1079, 1765
TspRI	CASTG	2 cut(s)	562, 1069
Van91I	CCANNNNNTGG	2 cut(s)	476, 1615
Vha464I	CTTAAG	1 cut(s)	761
VpaK11BI	GGWCC	1 cut(s)	757
XagI	CCTNNNNNAGG	1 cut(s)	998
XapI	RAATTY	7 cut(s)	503, 518, 635, 1112, 1177, 1895, 1924
XceI	RCATGY	3 cut(s)	1237, 1516, 1834
XmiI	GTMKAC	1 cut(s)	81
XmnI	GAANNNNTTC	3 cut(s)	522, 939, 1439
XspI	CTAG	9 cut(s)	38, 89, 386, 446, 998, 1085, 1400, 1983, 1987
Zsp2I	ATGCAT	1 cut(s)	1239

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.