Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0001152

Physical Location & Seq

Genomic Coordinates Forward (+)

84298071 .. 84300416

Gene Structure 2346 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000036771

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1536 bp

ATGCTGTTGATTGTGAAGAAAACACCTGAAGGAGAAGAAGTAAAGGAAATCTATATAGTTCCGATACTTCTTGGGGTTCTAGTCATGGCCTTTATCACCGACGCCCTAGGCCAAGTATATCAATTGGCTCCTTTTCTTCTAGGTTTAATCATACCAGATGGACCACCACTTCTTGGTGCAACATTAACAGCCAAGACCGAATTCTTGGTCTCAGAACTTCTTCTGTCAGTGTTCTTTTTTCTTATTGGCAACAGCGTAAATGTGTTTTTGATTGACGATTGGACTAAGTTTGCCAAACTTCAGATTATTATTCTTGTGTTATTTGTTACAAAGATAGTGGGAGTTACAGTGGCTGGCTTGTGCTGCAAAATTAGCTTGAAAAATAGTCTCATCCTCAGCATGATCATGAGTATGAAGGGCATAATAGAGTTTATGATTTATCATAAATGGATGACCATGAAGTTTATAGATGAACAATTTTTCACTCAGCTGATCTTGTCTGCACTAGTGATCACAATGATTGTAACACCAATTGTACAATTCTCATACACTCCTCAAAAACAACTCCGAAGATCAACTAAAGATTCACAGGTCAGAAACATCCAATCACAACGAAATAACTCCGGAACTATTCAGATTCTTTGTTGTGTTCACAATGAAGAAAGTGCCCGCAGCATCATCACCTTCTTAGAAGCTTCAAATCCAACAGAAGCAAGCCCCATATGCATGCACATTGTCCACGTAGTTGATTTATTTGGCAGTTCTGCACCACAATTGACACCTTACAAGAAGAAAAAGAAATTTAGGCGGTTGGATTCACCTACACATCAAATGATGCGGGCCTTCGAGAATTACTCAGAAAACTCAGGAGGACCAGTGATAATTCATCCATATACTATGGTGGCTCCTTATAAATCCATGCACGAGACCATTTTTCGTCTAGCTCGGGAAAAGCTCGCTCCTCTGATTATCATCTCATTCCCTGATATAACCACCACCATTGACAAAGGCTTGCCTTGTCGGCCAAGTGTCTCCAACTTCTCTTACATTGTGGCCGTATTTTTCATTGGTGGACCAGATGACCGTGAGACATTAGCATATGCATCACCCATGGTAGACAACCCTGACGTGGGCATCACAGTGTTCAGGATTCTAGTGCGTATCAAGAAGATAGACAGTTCTGAGGAAGAGGAGAGAGAGTTTAAGCTAGACGAGTCATTGTTGGATGAGTTTAAGCTAAGAAACACCAGGAATGATAGGTTGAATTGGCGTGATATTGAGGTGGATTATGGTGTTGAGACTGTTGAGGCTGTGAAGGCTATTAAAAATTTGCAGGGTGAGTACGACCTAGTGATGGTGGGCCGGCGACATTTGGATGTGTCGTTGAGGGATGAAGAAATGAGAGACTTATTGCACAATCCAGAGCTGGGAGTGATTGGTGACATGCTCGCTTCTTCAGATTTTTGTGGTGGAATGGTGAATGTGTTGGTGATGCAAGAAAGTAGGAGCTTGAGTTGTGGAGCTTATTGTAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0042592 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

512

Amino Acids

57.67

Weight (kDa)

5.93

Isoelectric Point (pI)

43.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	8 - 179	1.5e-17	Sodium/hydrogen exchanger, transmembrane
CHX17_C	PF23259	350 - 500	3.7e-09	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001152)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g43690 FvH4_7g25211 FvH4_7g25250 FvH4_7g25290 FvH4_7g25310
malus_domestica	MD01G1158700.v1.1 MD01G1158800.v1.1 MD07G1227200.v1.1 MD07G1227600.v1.1
prunus_persica	Prupe.2G257000_v2.0.a1 Prupe.2G257100_v2.0.a1
pyrus_communis	pycom01g17540
rosa_chinensis	RchiOBHm_Chr1g0371191 RchiOBHm_Chr1g0371201 RchiOBHm_Chr1g0371211
rosa_laevigata	RLG00000026978 RLG00000026979 RLG00000026980 RLG00000026981 RLG00000026983 RLG00000036771
rosa_multiflora	Rmu_co8222838.1_g000001 Rmu_sc0000631.1_g000023 Rmu_sc0001018.1_g000042 Rmu_sc0001018.1_g000044 Rmu_sc0001018.1_g000049 Rmu_sc0001018.1_g000050 Rmu_sc0001018.1_g000051 Rmu_sc0008081.1_g000003
rosa_roxburghii	Rroxscaffold_4G00285900 Rroxscaffold_4G00285910
rosa_rugosa	Rorug01G0361700 Rorug01G0361900 Rorug01G0362000 Rorug01G0362200 Rorug05G0460600
rosa_samantha	Rh1AG372000 Rh1AG372200 Rh1AG372300 Rh1BG335100 Rh1BG335500 Rh1BG335700 Rh1BG335800 Rh1CG348600 Rh1CG349000 Rh1CG349100 Rh1CG349200 Rh1DG366500 Rh1DG366900 Rh1DG367000 Rh5BG537300 Rh5CG560800 Rh5DG548900
rosa_wichuraiana	Rw1G032720 Rw1G032730 Rw1G032740 Rw5G047750 Rw5G047880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	912
AccB7I	CCANNNNNTGG	1 cut(s)	173
AccI	GTMKAC	1 cut(s)	1116
AccIII	TCCGGA	1 cut(s)	623
AciI	CCGC	3 cut(s)	670, 808, 838
AcoI	YGGCCR	2 cut(s)	1022, 1053
AcsI	RAATTY	3 cut(s)	200, 800, 1327
AcuI	CTGAAG	3 cut(s)	48, 284, 1440
AcyI	GRCGYC	1 cut(s)	102
AfaI	GTAC	2 cut(s)	537, 1343
AfiI	CCNNNNNNNGG	5 cut(s)	173, 1129, 1130, 1354, 1427
AgsI	TTSAA	3 cut(s)	379, 699, 1264
AhlI	ACTAGT	1 cut(s)	505
AjiI	CACGTC	1 cut(s)	1129
AjnI	CCWGG	1 cut(s)	1247
AjuI	GAANNNNNNNTTGG	2 cut(s)	185, 217
Alw26I	GTCTC	7 cut(s)	214, 392, 920, 1036, 1082, 1292, 1398
AlwNI	CAGNNNCTG	1 cut(s)	353
Ama87I	CYCGRG	1 cut(s)	945
Aor13HI	TCCGGA	1 cut(s)	623
AoxI	GGCC	6 cut(s)	87, 109, 840, 1022, 1053, 1360
ApeKI	GCWGC	2 cut(s)	363, 672
ApoI	RAATTY	3 cut(s)	200, 800, 1327
ArsI	GACNNNNNNTTYG	2 cut(s)	192, 224
Asp700I	GAANNNNTTC	1 cut(s)	219
AspA2I	CCTAGG	1 cut(s)	106
AspS9I	GGNCC	5 cut(s)	161, 840, 872, 1073, 1360
AsuHPI	GGTGA	8 cut(s)	88, 673, 810, 1098, 1349, 1451, 1489, 1501
AvaI	CYCGRG	1 cut(s)	945
AvaII	GGWCC	3 cut(s)	161, 872, 1073
AvrII	CCTAGG	1 cut(s)	106
BaeGI	GKGCMC	1 cut(s)	670
BauI	CACGAG	1 cut(s)	923
BbvCI	CCTCAGC	1 cut(s)	395
BbvI	GCAGC	2 cut(s)	350, 684
BccI	CCATC	2 cut(s)	152, 1348
BceAI	ACGGC	1 cut(s)	1040
BciT130I	CCWGG	1 cut(s)	1249
BclI	TGATCA	2 cut(s)	402, 510
BcoDI	GTCTC	7 cut(s)	214, 392, 920, 1036, 1082, 1292, 1398
BcuI	ACTAGT	1 cut(s)	505
BfaI	CTAG	8 cut(s)	80, 107, 140, 506, 941, 1154, 1208, 1349
BglI	GCCNNNNNGGC	1 cut(s)	1021
BisI	GCNGC	2 cut(s)	364, 673
BlnI	CCTAGG	1 cut(s)	106
BlsI	GCNGC	2 cut(s)	365, 674
Bme1390I	CCNGG	1 cut(s)	1249
Bme18I	GGWCC	3 cut(s)	161, 872, 1073
BmeT110I	CYCGRG	1 cut(s)	945
BmgBI	CACGTC	1 cut(s)	1129
BmgT120I	GGNCC	5 cut(s)	161, 840, 872, 1073, 1360
BmiI	GGNNCC	2 cut(s)	129, 906
BmrFI	CCNGG	1 cut(s)	1249
BmsI	GCATC	5 cut(s)	684, 825, 1112, 1143, 1482
BplI	GAGNNNNNCTC	2 cut(s)	839, 871
Bpu10I	CCTNAGC	1 cut(s)	395
BpuEI	CTTGAG	1 cut(s)	1531
BsaAI	YACGTR	1 cut(s)	742
BsaBI	GATNNNNATC	1 cut(s)	971
BsaHI	GRCGYC	1 cut(s)	102
BsaI	GGTCTC	2 cut(s)	214, 920
BsaJI	CCNNGG	2 cut(s)	106, 1110
BsaWI	WCCGGW	1 cut(s)	623
Bsc4I	CCNNNNNNNGG	5 cut(s)	173, 1129, 1130, 1354, 1427
Bse118I	RCCGGY	1 cut(s)	1362
Bse1I	ACTGG	1 cut(s)	875
Bse8I	GATNNNNATC	1 cut(s)	971
BseAI	TCCGGA	1 cut(s)	623
BseBI	CCWGG	1 cut(s)	1249
BseDI	CCNNGG	2 cut(s)	106, 1110
BseGI	GGATG	7 cut(s)	390, 456, 600, 886, 1231, 1381, 1396
BseJI	GATNNNNATC	1 cut(s)	971
BseLI	CCNNNNNNNGG	5 cut(s)	173, 1129, 1130, 1354, 1427
BseMII	CTCAG	6 cut(s)	225, 409, 500, 870, 879, 1173
BseNI	ACTGG	1 cut(s)	875
BseRI	GAGGAG	3 cut(s)	543, 951, 1205
BseSI	GKGCMC	1 cut(s)	670
BseXI	GCAGC	2 cut(s)	350, 684
BseYI	CCCAGC	1 cut(s)	1426
BsgI	GTGCAG	2 cut(s)	486, 750
BshFI	GGCC	6 cut(s)	89, 111, 842, 1024, 1055, 1362
BsiHKCI	CYCGRG	1 cut(s)	945
BsiSI	CCGG	2 cut(s)	624, 1363
BslI	CCNNNNNNNGG	5 cut(s)	173, 1129, 1130, 1354, 1427
BsmAI	GTCTC	7 cut(s)	214, 392, 920, 1036, 1082, 1292, 1398
BsnI	GGCC	6 cut(s)	89, 111, 842, 1024, 1055, 1362
Bso31I	GGTCTC	2 cut(s)	214, 920
BsoBI	CYCGRG	1 cut(s)	945
Bsp1286I	GDGCHC	1 cut(s)	670
Bsp13I	TCCGGA	1 cut(s)	623
Bsp1407I	TGTACA	1 cut(s)	535
Bsp143I	GATC	4 cut(s)	402, 492, 510, 572
Bsp19I	CCATGG	1 cut(s)	1110
BspACI	CCGC	3 cut(s)	670, 808, 838
BspANI	GGCC	6 cut(s)	89, 111, 842, 1024, 1055, 1362
BspCNI	CTCAG	6 cut(s)	224, 408, 499, 869, 878, 1174
BspEI	TCCGGA	1 cut(s)	623
BspHI	TCATGA	1 cut(s)	405
BspLI	GGNNCC	2 cut(s)	129, 906
BspTNI	GGTCTC	2 cut(s)	214, 920
BsrFI	RCCGGY	1 cut(s)	1362
BsrGI	TGTACA	1 cut(s)	535
BsrI	ACTGG	1 cut(s)	875
BssAI	RCCGGY	1 cut(s)	1362
BssECI	CCNNGG	2 cut(s)	106, 1110
BssMI	GATC	4 cut(s)	402, 492, 510, 572
BssNI	GRCGYC	1 cut(s)	102
BssSI	CACGAG	1 cut(s)	923
BssT1I	CCWWGG	2 cut(s)	106, 1110
Bst2BI	CACGAG	1 cut(s)	923
Bst2UI	CCWGG	1 cut(s)	1249
Bst4CI	ACNGT	5 cut(s)	349, 1085, 1141, 1178, 1303
Bst6I	CTCTTC	1 cut(s)	1182
BstACI	GRCGYC	1 cut(s)	102
BstAUI	TGTACA	1 cut(s)	535
BstBAI	YACGTR	1 cut(s)	742
BstC8I	GCNNGC	9 cut(s)	355, 670, 715, 728, 840, 957, 1013, 1364, 1449
BstDEI	CTNAG	9 cut(s)	211, 285, 395, 486, 688, 856, 865, 1182, 1238
BstDSI	CCRYGG	1 cut(s)	1110
BstF5I	GGATG	7 cut(s)	390, 456, 600, 886, 1231, 1381, 1396
BstKTI	GATC	4 cut(s)	405, 495, 513, 575
BstMAI	GTCTC	7 cut(s)	214, 392, 920, 1036, 1082, 1292, 1398
BstMBI	GATC	4 cut(s)	402, 492, 510, 572
BstMWI	GCNNNNNNNGC	5 cut(s)	363, 372, 723, 1021, 1316
BstNI	CCWGG	1 cut(s)	1249
BstNSI	RCATGY	2 cut(s)	730, 1447
BstSCI	CCNGG	1 cut(s)	1247
BstSLI	GKGCMC	1 cut(s)	670
BstV1I	GCAGC	2 cut(s)	350, 684
BsuRI	GGCC	6 cut(s)	89, 111, 842, 1024, 1055, 1362
BtgI	CCRYGG	1 cut(s)	1110
BtrI	CACGTC	1 cut(s)	1129
BtsCI	GGATG	7 cut(s)	390, 456, 600, 886, 1231, 1381, 1396
BtsIMutI	CAGTG	4 cut(s)	234, 354, 882, 1146
Cac8I	GCNNGC	9 cut(s)	355, 670, 715, 728, 840, 957, 1013, 1364, 1449
CaiI	CAGNNNCTG	1 cut(s)	353
CciI	TCATGA	1 cut(s)	405
Cfr10I	RCCGGY	1 cut(s)	1362
Cfr13I	GGNCC	5 cut(s)	161, 840, 872, 1073, 1360
CseI	GACGC	1 cut(s)	110
Csp6I	GTAC	2 cut(s)	536, 1342
CspCI	CAANNNNNGTGG	4 cut(s)	318, 353, 982, 1017
CviAII	CATG	8 cut(s)	85, 400, 406, 457, 727, 919, 1111, 1444
CviQI	GTAC	2 cut(s)	536, 1342
DdeI	CTNAG	9 cut(s)	211, 285, 395, 486, 688, 856, 865, 1182, 1238
DpnI	GATC	4 cut(s)	404, 494, 512, 574
DpnII	GATC	4 cut(s)	402, 492, 510, 572
EaeI	YGGCCR	2 cut(s)	1022, 1053
Eam1104I	CTCTTC	1 cut(s)	1182
EarI	CTCTTC	1 cut(s)	1182
Eco130I	CCWWGG	2 cut(s)	106, 1110
Eco31I	GGTCTC	2 cut(s)	214, 920
Eco47I	GGWCC	3 cut(s)	161, 872, 1073
Eco57I	CTGAAG	3 cut(s)	48, 284, 1440
Eco88I	CYCGRG	1 cut(s)	945
EcoRI	GAATTC	1 cut(s)	200
EcoRII	CCWGG	1 cut(s)	1247
EcoT14I	CCWWGG	2 cut(s)	106, 1110
EcoT22I	ATGCAT	2 cut(s)	728, 1105
ErhI	CCWWGG	2 cut(s)	106, 1110
FaeI	CATG	8 cut(s)	88, 403, 409, 460, 730, 922, 1114, 1447
FatI	CATG	8 cut(s)	84, 399, 405, 456, 726, 918, 1110, 1443
FauI	CCCGC	2 cut(s)	677, 831
FauNDI	CATATG	2 cut(s)	722, 1099
FbaI	TGATCA	2 cut(s)	402, 510
FblI	GTMKAC	1 cut(s)	1116
Fnu4HI	GCNGC	2 cut(s)	364, 673
FokI	GGATG	7 cut(s)	377, 463, 587, 873, 1238, 1388, 1403
Fsp4HI	GCNGC	2 cut(s)	364, 673
FspBI	CTAG	8 cut(s)	80, 107, 140, 506, 941, 1154, 1208, 1349
GluI	GCNGC	2 cut(s)	364, 673
GsaI	CCCAGC	1 cut(s)	1430
HaeIII	GGCC	6 cut(s)	89, 111, 842, 1024, 1055, 1362
HapII	CCGG	2 cut(s)	624, 1363
HgaI	GACGC	1 cut(s)	110
Hin1I	GRCGYC	1 cut(s)	102
Hin1II	CATG	8 cut(s)	88, 403, 409, 460, 730, 922, 1114, 1447
HindIII	AAGCTT	1 cut(s)	693
HinfI	GANTC	5 cut(s)	584, 637, 815, 1150, 1214
HpaII	CCGG	2 cut(s)	624, 1363
HphI	GGTGA	8 cut(s)	88, 673, 810, 1098, 1349, 1451, 1489, 1501
Hpy166II	GTNNAC	4 cut(s)	652, 739, 1073, 1117
Hpy188III	TCNNGA	8 cut(s)	406, 624, 847, 867, 947, 1147, 1165, 1421
Hpy8I	GTNNAC	4 cut(s)	652, 739, 1073, 1117
Hpy99I	CGWCG	1 cut(s)	104
HpyAV	CCTTC	5 cut(s)	23, 409, 694, 853, 1309
HpyCH4III	ACNGT	5 cut(s)	349, 1085, 1141, 1178, 1303
HpyCH4IV	ACGT	2 cut(s)	741, 1128
HpyF10VI	GCNNNNNNNGC	5 cut(s)	363, 372, 723, 1021, 1316
HpyF3I	CTNAG	9 cut(s)	211, 285, 395, 486, 688, 856, 865, 1182, 1238
HpySE526I	ACGT	2 cut(s)	741, 1128
Hsp92I	GRCGYC	1 cut(s)	102
Hsp92II	CATG	8 cut(s)	88, 403, 409, 460, 730, 922, 1114, 1447
Kpn2I	TCCGGA	1 cut(s)	623
KroI	GCCGGC	1 cut(s)	1362
KroNI	GCCGGC	1 cut(s)	1364
Ksp22I	TGATCA	2 cut(s)	402, 510
Kzo9I	GATC	4 cut(s)	402, 492, 510, 572
LmnI	GCTCC	5 cut(s)	133, 910, 964, 1506, 1520
Lsp1109I	GCAGC	2 cut(s)	350, 684
LweI	GCATC	5 cut(s)	684, 825, 1112, 1143, 1482
MaeI	CTAG	8 cut(s)	80, 107, 140, 506, 941, 1154, 1208, 1349
MaeII	ACGT	2 cut(s)	741, 1128
MaeIII	GTNAC	5 cut(s)	325, 343, 523, 1439, 1529
MalI	GATC	4 cut(s)	404, 494, 512, 574
MboI	GATC	4 cut(s)	402, 492, 510, 572
MfeI	CAATTG	3 cut(s)	122, 531, 773
MhlI	GDGCHC	1 cut(s)	670
MlyI	GAGTC	1 cut(s)	1223
MmeI	TCCRAC	4 cut(s)	728, 792, 1059, 1203
MnlI	CCTC	9 cut(s)	404, 564, 863, 972, 1177, 1183, 1273, 1300, 1380
Mph1103I	ATGCAT	2 cut(s)	728, 1105
MroI	TCCGGA	1 cut(s)	623
MroNI	GCCGGC	1 cut(s)	1362
MroXI	GAANNNNTTC	1 cut(s)	219
MseI	TTAA	5 cut(s)	146, 185, 1203, 1233, 1323
MslI	CAYNNNNRTG	5 cut(s)	404, 410, 725, 1139, 1374
MspA1I	CMGCKG	1 cut(s)	490
MspI	CCGG	2 cut(s)	624, 1363
MspR9I	CCNGG	1 cut(s)	1249
MunI	CAATTG	3 cut(s)	122, 531, 773
MvaI	CCWGG	1 cut(s)	1249
MwoI	GCNNNNNNNGC	5 cut(s)	363, 372, 723, 1021, 1316
NaeI	GCCGGC	1 cut(s)	1364
NcoI	CCATGG	1 cut(s)	1110
NdeI	CATATG	2 cut(s)	722, 1099
NdeII	GATC	4 cut(s)	402, 492, 510, 572
NgoMIV	GCCGGC	1 cut(s)	1362
NlaIII	CATG	8 cut(s)	88, 403, 409, 460, 730, 922, 1114, 1447
NlaIV	GGNNCC	2 cut(s)	129, 906
NmuCI	GTSAC	1 cut(s)	1439
NsiI	ATGCAT	2 cut(s)	728, 1105
NspI	RCATGY	2 cut(s)	730, 1447
PaeI	GCATGC	1 cut(s)	730
PagI	TCATGA	1 cut(s)	405
PdiI	GCCGGC	1 cut(s)	1364
PdmI	GAANNNNTTC	1 cut(s)	219
PfeI	GAWTC	4 cut(s)	584, 637, 815, 1150
PflMI	CCANNNNNTGG	1 cut(s)	173
PkrI	GCNGC	2 cut(s)	365, 674
PleI	GAGTC	1 cut(s)	1222
PpsI	GAGTC	1 cut(s)	1222
Ppu21I	YACGTR	1 cut(s)	742
PsiI	TTATAA	1 cut(s)	912
Psp6I	CCWGG	1 cut(s)	1247
PspFI	CCCAGC	1 cut(s)	1426
PspGI	CCWGG	1 cut(s)	1247
PspN4I	GGNNCC	2 cut(s)	129, 906
PspPI	GGNCC	5 cut(s)	161, 840, 872, 1073, 1360
PstNI	CAGNNNCTG	1 cut(s)	353
PvuII	CAGCTG	1 cut(s)	490
RsaI	GTAC	2 cut(s)	537, 1343
RsaNI	GTAC	2 cut(s)	536, 1342
RseI	CAYNNNNRTG	5 cut(s)	404, 410, 725, 1139, 1374
SaqAI	TTAA	5 cut(s)	146, 185, 1203, 1233, 1323
SatI	GCNGC	2 cut(s)	364, 673
Sau3AI	GATC	4 cut(s)	402, 492, 510, 572
Sau96I	GGNCC	5 cut(s)	161, 840, 872, 1073, 1360
SchI	GAGTC	1 cut(s)	1223
ScrFI	CCNGG	1 cut(s)	1249
SduI	GDGCHC	1 cut(s)	670
SfaNI	GCATC	5 cut(s)	684, 825, 1112, 1143, 1482
SinI	GGWCC	3 cut(s)	161, 872, 1073
SmiMI	CAYNNNNRTG	5 cut(s)	404, 410, 725, 1139, 1374
SmlI	CTYRAG	1 cut(s)	1510
SmoI	CTYRAG	1 cut(s)	1510
SpeI	ACTAGT	1 cut(s)	505
SphI	GCATGC	1 cut(s)	730
SsiI	CCGC	3 cut(s)	670, 808, 838
SspMI	CTAG	8 cut(s)	80, 107, 140, 506, 941, 1154, 1208, 1349
StyD4I	CCNGG	1 cut(s)	1247
StyI	CCWWGG	2 cut(s)	106, 1110
TaaI	ACNGT	5 cut(s)	349, 1085, 1141, 1178, 1303
TaiI	ACGT	2 cut(s)	744, 1131
TaqI	TCGA	1 cut(s)	846
TaqII	GACCGA	1 cut(s)	212
TatI	WGTACW	1 cut(s)	535
TfiI	GAWTC	4 cut(s)	584, 637, 815, 1150
Tru1I	TTAA	5 cut(s)	146, 185, 1203, 1233, 1323
Tru9I	TTAA	5 cut(s)	146, 185, 1203, 1233, 1323
TscAI	CASTG	4 cut(s)	234, 354, 882, 1146
TseFI	GTSAC	1 cut(s)	1439
TseI	GCWGC	2 cut(s)	363, 672
Tsp45I	GTSAC	1 cut(s)	1439
TspDTI	ATGAA	7 cut(s)	428, 473, 486, 672, 875, 1054, 1407
TspRI	CASTG	4 cut(s)	234, 354, 882, 1146
Van91I	CCANNNNNTGG	1 cut(s)	173
VpaK11BI	GGWCC	3 cut(s)	161, 872, 1073
XapI	RAATTY	3 cut(s)	200, 800, 1327
XceI	RCATGY	2 cut(s)	730, 1447
XmaJI	CCTAGG	1 cut(s)	106
XmiI	GTMKAC	1 cut(s)	1116
XmnI	GAANNNNTTC	1 cut(s)	219
XspI	CTAG	8 cut(s)	80, 107, 140, 506, 941, 1154, 1208, 1349
Zsp2I	ATGCAT	2 cut(s)	728, 1105

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.