Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb7

Orthogroup ID

OG0001152

Physical Location & Seq

Genomic Coordinates Forward (+)

19363196 .. 19366872

Gene Structure 3677 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_7g25310.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2379 bp

ATGGAAGCCATGAGAAATGACAGCGTCTGTTCACTGGGTTCCGGTTCGAAAGCCATTTGGCATGTTAATCCCCTAGATTTTCCATTTCCCCTAATGATGGTTCAAATCATTGTGATTGTTCTTACGTCCCAAGTTCTATATATCTGTATGAGGCCTTTGGGACAACATAAGTTTGTCTCCCATCTCCTGGCTGGCATAATCTTGGGGCCTTGTGTGATGGGGAACAATGCTAAATACCGCCGCATATTTTTGGGAGAGGAAGCAAATATGGGGTTGTTTGACACAATATCTAAAATAGGCGTGGTATACTCCCTATTCCTGGTCTCAGTGAAATTTGATGTATCCATGCTCAGAAGGACGGCGAAGAATGCCTGGAAGATTTCTCTAGCAAGCTTTATTTTTCCTCCGATTGTCTTCTTCGCCACTGTATACCCGATATTCAGCAAAGTTTCTGGATTCACCGGAGGATTTATGTTGCTCGTCTTGTTTGTGGGCACCTTAACTTTCAGCTTTTTTCCGGTAATAGTTCAGGTGTTGGAGGAACTCAACCTTATGACTTCGGAATTGGGTCAACTTGCCTTGTCCTCTTCGATGTTTAGCGATGCAATGCAATGGGTCTTCTCAGTAATCACCCTGGTAACAATGAAAAAGGATGCGGTTGAAGGAATAGGGACATTGTTTTCCTTATTGGCGTTGCTAGCTTTTAGTATCTTTGTCCTGCGCCCACTGATGAAATTGATCACTAAGAGATTACCGGAAGGAAAAGAAGTTGACCAAAATTTCGTAATCGCAGTACAACTCGGGGTTCTAGTTATGGCCTTCGTTTCCGACATTATAGGTATGAGGCATACTTCTGGTCCTATAATTCTGGGATTAGTCATGCCATATGGACAAGAACTGGGAGCAACAATAGTCGCCAGGACGGAAACCTTGGTAACAGAATTTTTTATGCCATTGTACTTTTTTCGAATCGGCTCGGGAATAGATGTGACTAGCATTTCCGATTGGAAGAGTTTTGCCAAGCTTGAGACTATAATTGTTGTAAGTTATGCTGCCAAGTTTATGGGAGTTTCAGTGGCCGGAATGTTCGCCAAACTTAGCTTCAAAAACAGTCTCGTCCTCAGCATGGTAATGAGTATCAAGGGACTAGTTGATCTCATAGCTTATACTCGATGGCGTGACATGCAGATCATTGATAAACAACTTTATACTCAACTCGTGTTGTCTATGGTTGGGATCACCATGCTGTTAACACCATTGATAAGATTTTTTTACGTTCTTAAAATACGAAGAGGATCATCAATGAGCGACCCACAGTTCAGACTTAATATTCAGTCATTGCCACCCAGGAATGAAATCTTTCGGATTCTCTGTTGTGTCCACAATGAAGAAAGTGTCCCCAACATCATCAACCTCCTGGAAGTCTCAAATCCAACGGAAGCGAGCCCGATTACTGCCTATGTTGTACATGCAGTCGAGTTCATAGGACGTTCTGCACCACTGCTAGTCCCTTACAAGCACCACAAAATTAGGCGGTCATATTCACTCACTAATAAAATGGTGCAAGCTTTCAAGAATTACTCCAAAAGCTCAAGAGGACCGGTCATGATTAGACCCTACACGATGGTCGCTCCTTATAAATCGATGCACGAGACTATTTCTCGTCTTGCTCTGGATAAGTTCGTACCTCTGATCATCATCCCATTCCACGAGAACCACCAGTCTCTTGTCCTCTCTAATATGACAGGCGCCATACGCCAATTCAACTATAATGTACAAGCATATGCACCGTGTACGGTGGGAATACTAGTTGACAGAGGCTTTCCTCTTCAGATGAATATTCACAACTTCACCTACAGTGTGGTTGTGTTTTTCATTGGTGGACCAGATGACCGTGAGGCATTAGCCTACGCGTCACGCATGCTCGACAACCCTAATGTGGAAATGACAGTGTTCAGATTCATCGTGCGTGTTATTGAGACAGAAACTAAACTAGACGAGGAGATAGAGTTCAGCCTCGACGAGTCGTTGATCGACGAGTTTAAGCTAGGGAACCTTGGGAATGAACGGTTGAATTGGAATGAGATCGAGGTGGAAAATGCTGTGCAGGTTATGACTGCCATTACCAAGTCGCAGGGACAATACGATCTTGCGATGGTTGGGCGACGACATGCGGATGTGTCGTTGAAGGATGAAGAAATGGTAGACTTTATGCAGAATCCAGAGCTCGGAGTGATAGGCGACATGCTTGCTTCTGCGGATTTTTGTAACGGAAGGGTGAACGTGTTGGTGATGCAAGAGAGCAGGGAGTTGAGAATTGGAGCTTTCCGTTCCGGCGGCTCCGGGAGAAATTCGGATTCTAAATCTGGGTTTAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0042592 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

793

Amino Acids

88.79

Weight (kDa)

8.46

Isoelectric Point (pI)

36.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	45 - 421	2e-37	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	513 - 608	6.2e-06	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	619 - 767	5.3e-09	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001152)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g43690 FvH4_7g25211 FvH4_7g25250 FvH4_7g25290 FvH4_7g25310
malus_domestica	MD01G1158700.v1.1 MD01G1158800.v1.1 MD07G1227200.v1.1 MD07G1227600.v1.1
prunus_persica	Prupe.2G257000_v2.0.a1 Prupe.2G257100_v2.0.a1
pyrus_communis	pycom01g17540
rosa_chinensis	RchiOBHm_Chr1g0371191 RchiOBHm_Chr1g0371201 RchiOBHm_Chr1g0371211
rosa_laevigata	RLG00000026978 RLG00000026979 RLG00000026980 RLG00000026981 RLG00000026983 RLG00000036771
rosa_multiflora	Rmu_co8222838.1_g000001 Rmu_sc0000631.1_g000023 Rmu_sc0001018.1_g000042 Rmu_sc0001018.1_g000044 Rmu_sc0001018.1_g000049 Rmu_sc0001018.1_g000050 Rmu_sc0001018.1_g000051 Rmu_sc0008081.1_g000003
rosa_roxburghii	Rroxscaffold_4G00285900 Rroxscaffold_4G00285910
rosa_rugosa	Rorug01G0361700 Rorug01G0361900 Rorug01G0362000 Rorug01G0362200 Rorug05G0460600
rosa_samantha	Rh1AG372000 Rh1AG372200 Rh1AG372300 Rh1BG335100 Rh1BG335500 Rh1BG335700 Rh1BG335800 Rh1CG348600 Rh1CG349000 Rh1CG349100 Rh1CG349200 Rh1DG366500 Rh1DG366900 Rh1DG367000 Rh5BG537300 Rh5CG560800 Rh5DG548900
rosa_wichuraiana	Rw1G032720 Rw1G032730 Rw1G032740 Rw5G047750 Rw5G047880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1638
Acc36I	ACCTGC	1 cut(s)	2098
AccB1I	GGYRCC	2 cut(s)	494, 1748
AccB7I	CCANNNNNTGG	1 cut(s)	187
AccI	GTMKAC	3 cut(s)	306, 429, 2205
AccII	CGCG	1 cut(s)	1913
AciI	CCGC	7 cut(s)	238, 241, 656, 1534, 2174, 2258, 2337
AclWI	GGATC	2 cut(s)	1244, 1303
AcoI	YGGCCR	1 cut(s)	1077
AcsI	RAATTY	4 cut(s)	332, 778, 941, 2350
AcuI	CTGAAG	1 cut(s)	1814
AcyI	GRCGYC	1 cut(s)	1749
AfaI	GTAC	6 cut(s)	795, 959, 1467, 1686, 1776, 1795
AfiI	CCNNNNNNNGG	6 cut(s)	97, 187, 319, 1126, 1939, 2228
AflIII	ACRYGT	2 cut(s)	1911, 2283
AgeI	ACCGGT	1 cut(s)	1600
AgsI	TTSAA	7 cut(s)	104, 662, 1105, 1573, 1765, 2074, 2188
AhlI	ACTAGT	2 cut(s)	1147, 1807
AjnI	CCWGG	7 cut(s)	186, 318, 371, 633, 917, 1346, 1416
AjuI	GAANNNNNNNTTGG	2 cut(s)	1085, 1117
AloI	GAACNNNNNNTCC	2 cut(s)	1994, 2026
Alw21I	GWGCWC	1 cut(s)	2229
Alw26I	GTCTC	8 cut(s)	181, 328, 1022, 1118, 1429, 1646, 1728, 1973
AlwI	GGATC	2 cut(s)	1244, 1303
AlwNI	CAGNNNCTG	1 cut(s)	27
Ama87I	CYCGRG	2 cut(s)	800, 976
AoxI	GGCC	4 cut(s)	152, 206, 816, 1077
ApeKI	GCWGC	1 cut(s)	1052
ApoI	RAATTY	4 cut(s)	332, 778, 941, 2350
ArsI	GACNNNNNNTTYG	2 cut(s)	764, 796
AsiGI	ACCGGT	1 cut(s)	1600
Asp700I	GAANNNNTTC	1 cut(s)	1359
AspLEI	GCGC	2 cut(s)	723, 1751
AspS9I	GGNCC	4 cut(s)	206, 857, 1598, 1883
AsuC2I	CCSGG	1 cut(s)	2344
AsuHPI	GGTGA	6 cut(s)	451, 622, 1231, 1843, 2290, 2302
AsuII	TTCGAA	2 cut(s)	47, 967
AsuNHI	GCTAGC	1 cut(s)	697
AvaI	CYCGRG	2 cut(s)	800, 976
AvaII	GGWCC	3 cut(s)	857, 1598, 1883
BaeGI	GKGCMC	1 cut(s)	497
BanI	GGYRCC	2 cut(s)	494, 1748
BanII	GRGCYC	2 cut(s)	1448, 2229
BauI	CACGAG	3 cut(s)	1217, 1649, 1709
BbsI	GAAGAC	2 cut(s)	406, 610
Bbv12I	GWGCWC	1 cut(s)	2229
BbvCI	CCTCAGC	1 cut(s)	1121
BbvI	GCAGC	1 cut(s)	1039
BccI	CCATC	6 cut(s)	91, 189, 211, 1167, 1618, 2149
BceAI	ACGGC	1 cut(s)	375
BcgI	CGANNNNNNTGC	2 cut(s)	2231, 2265
BciT130I	CCWGG	7 cut(s)	188, 320, 373, 635, 919, 1348, 1418
BciVI	GTATCC	1 cut(s)	352
BclI	TGATCA	2 cut(s)	738, 1692
BcnI	CCSGG	1 cut(s)	2344
BcoDI	GTCTC	8 cut(s)	181, 328, 1022, 1118, 1429, 1646, 1728, 1973
BcuI	ACTAGT	2 cut(s)	1147, 1807
BfmI	CTRYAG	1 cut(s)	1855
BfoI	RGCGCY	1 cut(s)	1752
BfuAI	ACCTGC	1 cut(s)	2098
BfuI	GTATCC	1 cut(s)	352
BisI	GCNGC	3 cut(s)	241, 1053, 2338
BlsI	GCNGC	3 cut(s)	242, 1054, 2339
Bme1390I	CCNGG	8 cut(s)	188, 320, 373, 635, 919, 1348, 1418, 2344
Bme18I	GGWCC	3 cut(s)	857, 1598, 1883
BmeT110I	CYCGRG	2 cut(s)	800, 976
BmgT120I	GGNCC	4 cut(s)	206, 857, 1598, 1883
BmiI	GGNNCC	6 cut(s)	40, 207, 496, 1750, 2054, 2341
BmrFI	CCNGG	8 cut(s)	188, 320, 373, 635, 919, 1348, 1418, 2344
BmrI	ACTGGG	2 cut(s)	44, 908
BmsI	GCATC	4 cut(s)	592, 643, 1635, 2283
BmtI	GCTAGC	1 cut(s)	701
BmuI	ACTGGG	2 cut(s)	44, 908
BpiI	GAAGAC	2 cut(s)	406, 610
Bpu10I	CCTNAGC	1 cut(s)	1121
Bpu14I	TTCGAA	2 cut(s)	47, 967
BpuEI	CTTGAG	2 cut(s)	1046, 1576
BpuMI	CCSGG	1 cut(s)	2344
Bsa29I	ATCGAT	1 cut(s)	1643
BsaBI	GATNNNNATC	1 cut(s)	1697
BsaHI	GRCGYC	1 cut(s)	1749
BsaI	GGTCTC	1 cut(s)	328
BsaJI	CCNNGG	4 cut(s)	633, 930, 1346, 2056
BsaWI	WCCGGW	5 cut(s)	41, 461, 517, 754, 1600
BsaXI	ACNNNNNCTCC	4 cut(s)	1059, 1089, 1994, 2024
Bsc4I	CCNNNNNNNGG	6 cut(s)	97, 187, 319, 1126, 1939, 2228
Bse118I	RCCGGY	1 cut(s)	1600
Bse1I	ACTGG	3 cut(s)	39, 903, 1720
Bse3DI	GCAATG	3 cut(s)	612, 617, 1337
Bse8I	GATNNNNATC	1 cut(s)	1697
BseBI	CCWGG	7 cut(s)	188, 320, 373, 635, 919, 1348, 1418
BseCI	ATCGAT	1 cut(s)	1643
BseDI	CCNNGG	4 cut(s)	633, 930, 1346, 2056
BseGI	GGATG	4 cut(s)	658, 1698, 2182, 2197
BseJI	GATNNNNATC	1 cut(s)	1697
BseLI	CCNNNNNNNGG	6 cut(s)	97, 187, 319, 1126, 1939, 2228
BseMI	GCAATG	3 cut(s)	612, 617, 1337
BseMII	CTCAG	4 cut(s)	339, 364, 636, 1135
BseNI	ACTGG	3 cut(s)	39, 903, 1720
BseRI	GAGGAG	1 cut(s)	2015
BseSI	GKGCMC	1 cut(s)	497
BseXI	GCAGC	1 cut(s)	1039
BsgI	GTGCAG	2 cut(s)	1479, 2126
Bsh1236I	CGCG	1 cut(s)	1913
BshFI	GGCC	4 cut(s)	154, 208, 818, 1079
BshNI	GGYRCC	2 cut(s)	494, 1748
BshTI	ACCGGT	1 cut(s)	1600
BshVI	ATCGAT	1 cut(s)	1643
BsiHKAI	GWGCWC	1 cut(s)	2229
BsiHKCI	CYCGRG	2 cut(s)	800, 976
BsiSI	CCGG	8 cut(s)	42, 462, 518, 755, 1080, 1601, 2334, 2343
BslFI	GGGAC	7 cut(s)	112, 174, 685, 1158, 1382, 1493, 2151
BslI	CCNNNNNNNGG	6 cut(s)	97, 187, 319, 1126, 1939, 2228
BsmAI	GTCTC	8 cut(s)	181, 328, 1022, 1118, 1429, 1646, 1728, 1973
BsmFI	GGGAC	7 cut(s)	112, 174, 685, 1158, 1382, 1493, 2151
BsmI	GAATGC	1 cut(s)	373
BsnI	GGCC	4 cut(s)	154, 208, 818, 1079
Bso31I	GGTCTC	1 cut(s)	328
BsoBI	CYCGRG	2 cut(s)	800, 976
Bsp119I	TTCGAA	2 cut(s)	47, 967
Bsp1286I	GDGCHC	3 cut(s)	497, 1448, 2229
Bsp1407I	TGTACA	2 cut(s)	1465, 1774
Bsp143I	GATC	9 cut(s)	738, 1153, 1188, 1236, 1295, 1692, 2031, 2085, 2146
BspACI	CCGC	7 cut(s)	238, 241, 656, 1534, 2174, 2258, 2337
BspANI	GGCC	4 cut(s)	154, 208, 818, 1079
BspCNI	CTCAG	4 cut(s)	338, 363, 635, 1134
BspDI	ATCGAT	1 cut(s)	1643
BspFNI	CGCG	1 cut(s)	1913
BspHI	TCATGA	1 cut(s)	1605
BspLI	GGNNCC	6 cut(s)	40, 207, 496, 1750, 2054, 2341
BspMI	ACCTGC	1 cut(s)	2098
BspOI	GCTAGC	1 cut(s)	701
BspPI	GGATC	2 cut(s)	1244, 1303
BspT104I	TTCGAA	2 cut(s)	47, 967
BspT107I	GGYRCC	2 cut(s)	494, 1748
BspTNI	GGTCTC	1 cut(s)	328
BsrDI	GCAATG	3 cut(s)	612, 617, 1337
BsrFI	RCCGGY	1 cut(s)	1600
BsrGI	TGTACA	2 cut(s)	1465, 1774
BsrI	ACTGG	3 cut(s)	39, 903, 1720
BssAI	RCCGGY	1 cut(s)	1600
BssECI	CCNNGG	4 cut(s)	633, 930, 1346, 2056
BssMI	GATC	9 cut(s)	738, 1153, 1188, 1236, 1295, 1692, 2031, 2085, 2146
BssNAI	GTATAC	2 cut(s)	307, 430
BssNI	GRCGYC	1 cut(s)	1749
BssSI	CACGAG	3 cut(s)	1217, 1649, 1709
BssT1I	CCWWGG	2 cut(s)	930, 2056
Bst1107I	GTATAC	2 cut(s)	307, 430
Bst2BI	CACGAG	3 cut(s)	1217, 1649, 1709
Bst2UI	CCWGG	7 cut(s)	188, 320, 373, 635, 919, 1348, 1418
Bst4CI	ACNGT	9 cut(s)	427, 1112, 1317, 1791, 1798, 1859, 1895, 1951, 2070
Bst6I	CTCTTC	4 cut(s)	592, 1004, 1285, 1833
BstACI	GRCGYC	1 cut(s)	1749
BstAUI	TGTACA	2 cut(s)	1465, 1774
BstBI	TTCGAA	2 cut(s)	47, 967
BstC8I	GCNNGC	7 cut(s)	193, 391, 699, 1444, 1566, 1922, 2250
BstDEI	CTNAG	6 cut(s)	325, 350, 622, 744, 1097, 1121
BstF5I	GGATG	4 cut(s)	658, 1698, 2182, 2197
BstFNI	CGCG	1 cut(s)	1913
BstH2I	RGCGCY	1 cut(s)	1752
BstHHI	GCGC	2 cut(s)	723, 1751
BstKTI	GATC	9 cut(s)	741, 1156, 1191, 1239, 1298, 1695, 2034, 2088, 2149
BstMAI	GTCTC	8 cut(s)	181, 328, 1022, 1118, 1429, 1646, 1728, 1973
BstMBI	GATC	9 cut(s)	738, 1153, 1188, 1236, 1295, 1692, 2031, 2085, 2146
BstMWI	GCNNNNNNNGC	4 cut(s)	368, 698, 1183, 1755
BstNI	CCWGG	7 cut(s)	188, 320, 373, 635, 919, 1348, 1418
BstNSI	RCATGY	6 cut(s)	65, 1186, 1472, 1924, 2174, 2248
BstSCI	CCNGG	8 cut(s)	186, 318, 371, 633, 917, 1346, 1416, 2342
BstSFI	CTRYAG	1 cut(s)	1855
BstSLI	GKGCMC	1 cut(s)	497
BstUI	CGCG	1 cut(s)	1913
BstV1I	GCAGC	1 cut(s)	1039
BstV2I	GAAGAC	2 cut(s)	406, 610
BstXI	CCANNNNNNTGG	1 cut(s)	1063
BstZ17I	GTATAC	2 cut(s)	307, 430
Bsu15I	ATCGAT	1 cut(s)	1643
BsuI	GTATCC	1 cut(s)	352
BsuRI	GGCC	4 cut(s)	154, 208, 818, 1079
BsuTUI	ATCGAT	1 cut(s)	1643
BtgZI	GCGATG	2 cut(s)	615, 2168
BtsCI	GGATG	4 cut(s)	658, 1698, 2182, 2197
BtsI	GCAGTG	1 cut(s)	1499
BtsIMutI	CAGTG	8 cut(s)	32, 333, 423, 725, 1080, 1499, 1864, 1956
BveI	ACCTGC	1 cut(s)	2098
Cac8I	GCNNGC	7 cut(s)	193, 391, 699, 1444, 1566, 1922, 2250
CaiI	CAGNNNCTG	1 cut(s)	27
CciI	TCATGA	1 cut(s)	1605
CfoI	GCGC	2 cut(s)	723, 1751
Cfr10I	RCCGGY	1 cut(s)	1600
Cfr13I	GGNCC	4 cut(s)	206, 857, 1598, 1883
ClaI	ATCGAT	1 cut(s)	1643
CseI	GACGC	2 cut(s)	13, 1902
Csp6I	GTAC	6 cut(s)	794, 958, 1466, 1685, 1775, 1794
CspAI	ACCGGT	1 cut(s)	1600
CviQI	GTAC	6 cut(s)	794, 958, 1466, 1685, 1775, 1794
DdeI	CTNAG	6 cut(s)	325, 350, 622, 744, 1097, 1121
DinI	GGCGCC	1 cut(s)	1750
DpnI	GATC	9 cut(s)	740, 1155, 1190, 1238, 1297, 1694, 2033, 2087, 2148
DpnII	GATC	9 cut(s)	738, 1153, 1188, 1236, 1295, 1692, 2031, 2085, 2146
EaeI	YGGCCR	1 cut(s)	1077
Eam1104I	CTCTTC	4 cut(s)	592, 1004, 1285, 1833
EarI	CTCTTC	4 cut(s)	592, 1004, 1285, 1833
Ecl136II	GAGCTC	1 cut(s)	2227
Eco130I	CCWWGG	2 cut(s)	930, 2056
Eco147I	AGGCCT	1 cut(s)	154
Eco24I	GRGCYC	2 cut(s)	1448, 2229
Eco31I	GGTCTC	1 cut(s)	328
Eco47I	GGWCC	3 cut(s)	857, 1598, 1883
Eco53kI	GAGCTC	1 cut(s)	2227
Eco57I	CTGAAG	1 cut(s)	1814
Eco88I	CYCGRG	2 cut(s)	800, 976
EcoICRI	GAGCTC	1 cut(s)	2227
EcoO109I	RGGNCCY	1 cut(s)	206
EcoRII	CCWGG	7 cut(s)	186, 318, 371, 633, 917, 1346, 1416
EcoT14I	CCWWGG	2 cut(s)	930, 2056
EcoT38I	GRGCYC	2 cut(s)	1448, 2229
EgeI	GGCGCC	1 cut(s)	1750
EheI	GGCGCC	1 cut(s)	1750
ErhI	CCWWGG	2 cut(s)	930, 2056
FaqI	GGGAC	7 cut(s)	112, 174, 685, 1158, 1382, 1493, 2151
FauNDI	CATATG	2 cut(s)	886, 1783
FbaI	TGATCA	2 cut(s)	738, 1692
FblI	GTMKAC	3 cut(s)	306, 429, 2205
Fnu4HI	GCNGC	3 cut(s)	241, 1053, 2338
FokI	GGATG	4 cut(s)	665, 1685, 2189, 2204
FriOI	GRGCYC	2 cut(s)	1448, 2229
Fsp4HI	GCNGC	3 cut(s)	241, 1053, 2338
GlaI	GCGC	2 cut(s)	722, 1750
GluI	GCNGC	3 cut(s)	241, 1053, 2338
HaeII	RGCGCY	1 cut(s)	1752
HaeIII	GGCC	4 cut(s)	154, 208, 818, 1079
HapII	CCGG	8 cut(s)	42, 462, 518, 755, 1080, 1601, 2334, 2343
HgaI	GACGC	2 cut(s)	13, 1902
HhaI	GCGC	2 cut(s)	723, 1751
Hin1I	GRCGYC	1 cut(s)	1749
Hin6I	GCGC	2 cut(s)	721, 1749
HinP1I	GCGC	2 cut(s)	721, 1749
HincII	GTYRAC	4 cut(s)	572, 772, 1251, 1813
HindII	GTYRAC	4 cut(s)	572, 772, 1251, 1813
HindIII	AAGCTT	3 cut(s)	391, 1022, 1566
HinfI	GANTC	7 cut(s)	456, 969, 1366, 1959, 2024, 2218, 2357
HpaI	GTTAAC	1 cut(s)	1251
HpaII	CCGG	8 cut(s)	42, 462, 518, 755, 1080, 1601, 2334, 2343
HphI	GGTGA	6 cut(s)	451, 622, 1231, 1843, 2290, 2302
Hpy188III	TCNNGA	7 cut(s)	453, 978, 1573, 1593, 1606, 1673, 2222
Hpy99I	CGWCG	3 cut(s)	2024, 2039, 2169
HpyAV	CCTTC	6 cut(s)	348, 656, 752, 829, 2182, 2268
HpyCH4III	ACNGT	9 cut(s)	427, 1112, 1317, 1791, 1798, 1859, 1895, 1951, 2070
HpyCH4IV	ACGT	4 cut(s)	125, 1275, 1489, 2283
HpyF10VI	GCNNNNNNNGC	4 cut(s)	368, 698, 1183, 1755
HpyF3I	CTNAG	6 cut(s)	325, 350, 622, 744, 1097, 1121
HpySE526I	ACGT	4 cut(s)	125, 1275, 1489, 2283
Hsp92I	GRCGYC	1 cut(s)	1749
HspAI	GCGC	2 cut(s)	721, 1749
KasI	GGCGCC	1 cut(s)	1748
Ksp22I	TGATCA	2 cut(s)	738, 1692
KspAI	GTTAAC	1 cut(s)	1251
Kzo9I	GATC	9 cut(s)	738, 1153, 1188, 1236, 1295, 1692, 2031, 2085, 2146
LmnI	GCTCC	4 cut(s)	902, 1636, 2321, 2345
Lsp1109I	GCAGC	1 cut(s)	1039
LweI	GCATC	4 cut(s)	592, 643, 1635, 2283
MaeII	ACGT	4 cut(s)	125, 1275, 1489, 2283
MaeIII	GTNAC	6 cut(s)	637, 934, 988, 1178, 1914, 2267
MalI	GATC	9 cut(s)	740, 1155, 1190, 1238, 1297, 1694, 2033, 2087, 2148
MboI	GATC	9 cut(s)	738, 1153, 1188, 1236, 1295, 1692, 2031, 2085, 2146
MhlI	GDGCHC	3 cut(s)	497, 1448, 2229
MluI	ACGCGT	1 cut(s)	1911
Mly113I	GGCGCC	1 cut(s)	1749
MlyI	GAGTC	1 cut(s)	2033
MmeI	TCCRAC	3 cut(s)	516, 852, 1457
MroXI	GAANNNNTTC	1 cut(s)	1359
MseI	TTAA	8 cut(s)	66, 500, 1250, 1281, 1326, 2043, 2373, 2377
MslI	CAYNNNNRTG	2 cut(s)	1130, 2175
MspI	CCGG	8 cut(s)	42, 462, 518, 755, 1080, 1601, 2334, 2343
MspR9I	CCNGG	8 cut(s)	188, 320, 373, 635, 919, 1348, 1418, 2344
Mva1269I	GAATGC	1 cut(s)	373
MvaI	CCWGG	7 cut(s)	188, 320, 373, 635, 919, 1348, 1418
MvnI	CGCG	1 cut(s)	1913
MwoI	GCNNNNNNNGC	4 cut(s)	368, 698, 1183, 1755
NarI	GGCGCC	1 cut(s)	1749
NciI	CCSGG	1 cut(s)	2344
NdeI	CATATG	2 cut(s)	886, 1783
NdeII	GATC	9 cut(s)	738, 1153, 1188, 1236, 1295, 1692, 2031, 2085, 2146
NheI	GCTAGC	1 cut(s)	697
NlaIV	GGNNCC	6 cut(s)	40, 207, 496, 1750, 2054, 2341
NmuCI	GTSAC	3 cut(s)	988, 1178, 1914
NspI	RCATGY	6 cut(s)	65, 1186, 1472, 1924, 2174, 2248
NspV	TTCGAA	2 cut(s)	47, 967
PacI	TTAATTAA	1 cut(s)	2377
PaeI	GCATGC	1 cut(s)	1924
PagI	TCATGA	1 cut(s)	1605
PceI	AGGCCT	1 cut(s)	154
PctI	GAATGC	1 cut(s)	373
PdmI	GAANNNNTTC	1 cut(s)	1359
PfeI	GAWTC	6 cut(s)	456, 969, 1366, 1959, 2218, 2357
PflFI	GACNNNGTC	1 cut(s)	23
PflMI	CCANNNNNTGG	1 cut(s)	187
PfoI	TCCNGGA	2 cut(s)	1416, 2342
PinAI	ACCGGT	1 cut(s)	1600
PkrI	GCNGC	3 cut(s)	242, 1054, 2339
PleI	GAGTC	1 cut(s)	2032
PluTI	GGCGCC	1 cut(s)	1752
PpsI	GAGTC	1 cut(s)	2032
PsiI	TTATAA	1 cut(s)	1638
Psp124BI	GAGCTC	1 cut(s)	2229
Psp6I	CCWGG	7 cut(s)	186, 318, 371, 633, 917, 1346, 1416
PspGI	CCWGG	7 cut(s)	186, 318, 371, 633, 917, 1346, 1416
PspN4I	GGNNCC	6 cut(s)	40, 207, 496, 1750, 2054, 2341
PspPI	GGNCC	4 cut(s)	206, 857, 1598, 1883
PstNI	CAGNNNCTG	1 cut(s)	27
PsyI	GACNNNGTC	1 cut(s)	23
RsaI	GTAC	6 cut(s)	795, 959, 1467, 1686, 1776, 1795
RsaNI	GTAC	6 cut(s)	794, 958, 1466, 1685, 1775, 1794
RseI	CAYNNNNRTG	2 cut(s)	1130, 2175
SacI	GAGCTC	1 cut(s)	2229
SaqAI	TTAA	8 cut(s)	66, 500, 1250, 1281, 1326, 2043, 2373, 2377
SatI	GCNGC	3 cut(s)	241, 1053, 2338
Sau3AI	GATC	9 cut(s)	738, 1153, 1188, 1236, 1295, 1692, 2031, 2085, 2146
Sau96I	GGNCC	4 cut(s)	206, 857, 1598, 1883
SchI	GAGTC	1 cut(s)	2033
ScrFI	CCNGG	8 cut(s)	188, 320, 373, 635, 919, 1348, 1418, 2344
SduI	GDGCHC	3 cut(s)	497, 1448, 2229
SfaNI	GCATC	4 cut(s)	592, 643, 1635, 2283
SfcI	CTRYAG	1 cut(s)	1855
SfoI	GGCGCC	1 cut(s)	1750
SfuI	TTCGAA	2 cut(s)	47, 967
SinI	GGWCC	3 cut(s)	857, 1598, 1883
SmiMI	CAYNNNNRTG	2 cut(s)	1130, 2175
SmlI	CTYRAG	2 cut(s)	1025, 1591
SmoI	CTYRAG	2 cut(s)	1025, 1591
SpeI	ACTAGT	2 cut(s)	1147, 1807
SphI	GCATGC	1 cut(s)	1924
SseBI	AGGCCT	1 cut(s)	154
SsiI	CCGC	7 cut(s)	238, 241, 656, 1534, 2174, 2258, 2337
SspDI	GGCGCC	1 cut(s)	1748
SspI	AATATT	2 cut(s)	1330, 1840
SstI	GAGCTC	1 cut(s)	2229
StuI	AGGCCT	1 cut(s)	154
StyD4I	CCNGG	8 cut(s)	186, 318, 371, 633, 917, 1346, 1416, 2342
StyI	CCWWGG	2 cut(s)	930, 2056
TaaI	ACNGT	9 cut(s)	427, 1112, 1317, 1791, 1798, 1859, 1895, 1951, 2070
TaiI	ACGT	4 cut(s)	128, 1278, 1492, 2286
TatI	WGTACW	4 cut(s)	793, 957, 1465, 1774
TauI	GCSGC	2 cut(s)	243, 2340
TfiI	GAWTC	6 cut(s)	456, 969, 1366, 1959, 2218, 2357
Tru1I	TTAA	8 cut(s)	66, 500, 1250, 1281, 1326, 2043, 2373, 2377
Tru9I	TTAA	8 cut(s)	66, 500, 1250, 1281, 1326, 2043, 2373, 2377
TscAI	CASTG	8 cut(s)	39, 333, 430, 732, 1080, 1506, 1864, 1956
TseFI	GTSAC	3 cut(s)	988, 1178, 1914
TseI	GCWGC	1 cut(s)	1052
Tsp45I	GTSAC	3 cut(s)	988, 1178, 1914
TspGWI	ACGGA	4 cut(s)	938, 1451, 2286, 2318
TspRI	CASTG	8 cut(s)	39, 333, 430, 732, 1080, 1506, 1864, 1956
Tth111I	GACNNNGTC	1 cut(s)	23
Van91I	CCANNNNNTGG	1 cut(s)	187
VpaK11BI	GGWCC	3 cut(s)	857, 1598, 1883
XapI	RAATTY	4 cut(s)	332, 778, 941, 2350
XceI	RCATGY	6 cut(s)	65, 1186, 1472, 1924, 2174, 2248
XcmI	CCANNNNNNNNNTGG	1 cut(s)	188
XmiI	GTMKAC	3 cut(s)	306, 429, 2205
XmnI	GAANNNNTTC	1 cut(s)	1359

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.