Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0001152

Physical Location & Seq

Genomic Coordinates Reverse (-)

7079830 .. 7087623

Gene Structure 7794 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00285910.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2226 bp

ATGGAAGCCTCTCGCACACGAGTTCGGTGCCGTAACGATGGCAAGTCGGCGACCGAGGGAGGTGAGGCGAGTGTGGCGATTGCTAAAGGGCGTAACCAGGTTATAAGGGACAAACTATTTAATCCGAAACATGCGTCGTTGTGGGCAACAATGGGTCTGCTGGGCGCTACATATTCCATGTTCATAACTGCAGTAAAATTTGACATAAGCATGATCAAAGGGACAAAGCGTTCTTTGAAGATTGGTGTTCCAGGGTTGCTTATTACCGTTGCAGTATCCTTAGGCCTCGGATACCCGCTAGCCAGCTGCATTGCTGGAATCAAAGGGGGATTCTTCCCCATATTTTTATTTTCTATTATTTCTTTCACTTTCTTCCCTGATATAGCTCCGGCCTTAGAGGACCTCAACCTTATGAATTCTGAGTTGGGTCAAATCGCCATGTCCTCGGCTTTGCATTTTGAAATGATACAATGGTTGTTCTCAACAGTACAAGACATAGCTACTAAAGATAAAGCAATCCACGGAGCTTTGACATTCATTTCCGCAGTGGTATTGCTTGTTTTCATTGTTTATGTCATACGCCCGCTTATGGTATCAATTGCTAAGAACACACCCGAAGGACAACAAGTAAAGGAGTTTTATGTAGTAGCAATACTAGTTGGGGCACTAGTCCTGTCGTTTATATCCGACGCAATAGGTTGCACCTTTGTTCTTGGTCCTGTAATTCTTGGGTTTGTCATACCAGATGGACCACCTCTGGGTGCAACATTAGTAACCAAGACTGAATTGTTGGTCTCTGAACTTCTTCTTCCCGTGTTCTTTTTTCGTATTGGCTTCAGGATAAACATACTGTCGATTAGCGATTGGAAGAGTTTTACCACATTGCAGGCCATCATTGGTGCATCATATGTGGCAAAGATGGTGGCAGTTACCGGGGCTGCATTGTGCTGCAAAATTAGTTTGAGAAATAGTCTCTTGCTCGGCATAATATTGAATATCCGGGGTATTATAGATTTGACCCTTTTCTCTCGATGGAGGTATAGTCAGCTCCTAAATGAACACGTTTATACTCAACTAGTGTTATCTGTGCTGATTGGGAACATGATTGCAACATCATTGGTACATTTCACATACAGACCTCATTTAAGACTGGGGCCATCACCCAAAGGTCCACGAATTAGAAACATTCAATCAGTTCAAAGCAACTCTCCTTTCGACATTCTTTGTTGTATCCACAACGAGGAAAGTATCCGCAACCTCACCTCCCTCCTAGAAGCCTCAAACCCAACAGAAGCAAGCCCTATTTGCGCTTACATTATCCAAGCAGTTGAGCTTGTGGGGCGGGCTGTACCTGTACTAGTCCCTTATAAGAAGAAGAAGGAGGGAAACAAATTTTACCGAATAAATTCACTCACACATCATATGATGCAAGCCTTTTTGAACTACTCGGAAAACTCAAGTGGACCTGTCTTGATCCAATCATTCACTATGATCGCGCCCTATAAATCGATGCATGAGACCATTTGCCGTCTAGTGGATGACAATCTCGTACCTTTGGTTATCCTCCCATTTCATGAAAACCACCAGGTTATGGTTGACACCAACGTGACTGCCTCAATACGACAATTCAACAACAATGTGCAGGAATATTCATCGTGTACAGTTGGAATACTAGTTGAAAGAGGGTTGCCAGGTCGGCTGACCATGTCCCATTTCTCATATAATGTGGCTACATTTTTCGTTGGCGGACCAGATGACCGCGAGGCTTTGGCCTTAGCAGCACGTATGTTAGGCAACCAGAATGTGGTCCTCACAGTATACAAGTTTATTGTGCGGAAAAAATTAAGCGAGGGTGACTATAATGAAGACGAGGAGATGGAGACCGGGCTGGATGAGTCGATGGTGGATGAGTTTAAGCTGAGGAATAATGGGAATGACGGCTTGAATTGGCGAGATATCGAGGTTGAGGACTCTATACAGCTTATTGATGCAATTATGAACTCGCAGGGGGATTATGATCTTGTAATGGTTGGAAGGCGGCATGCGGACAGTTCATTGAGGGATGAGGAAATGTCAGAATTTGTGCATTATGCGGAGCTGGGAATGATCGGTGACATGCTTGCTTCTTCAGATTTCTGTGGTGGAATGGTGAATGTTTTGGTGTTGCAAGAAAGCAAGGAACTTAGTAGAAATTATGGATCAGCTTTTCGAAAGATTTGGGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0042592 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

741

Amino Acids

82.02

Weight (kDa)

6.34

Isoelectric Point (pI)

38.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	50 - 374	1.7e-27	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	437 - 568	3.7e-06	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	574 - 724	6.8e-06	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001152)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g43690 FvH4_7g25211 FvH4_7g25250 FvH4_7g25290 FvH4_7g25310
malus_domestica	MD01G1158700.v1.1 MD01G1158800.v1.1 MD07G1227200.v1.1 MD07G1227600.v1.1
prunus_persica	Prupe.2G257000_v2.0.a1 Prupe.2G257100_v2.0.a1
pyrus_communis	pycom01g17540
rosa_chinensis	RchiOBHm_Chr1g0371191 RchiOBHm_Chr1g0371201 RchiOBHm_Chr1g0371211
rosa_laevigata	RLG00000026978 RLG00000026979 RLG00000026980 RLG00000026981 RLG00000026983 RLG00000036771
rosa_multiflora	Rmu_co8222838.1_g000001 Rmu_sc0000631.1_g000023 Rmu_sc0001018.1_g000042 Rmu_sc0001018.1_g000044 Rmu_sc0001018.1_g000049 Rmu_sc0001018.1_g000050 Rmu_sc0001018.1_g000051 Rmu_sc0008081.1_g000003
rosa_roxburghii	Rroxscaffold_4G00285900 Rroxscaffold_4G00285910
rosa_rugosa	Rorug01G0361700 Rorug01G0361900 Rorug01G0362000 Rorug01G0362200 Rorug05G0460600
rosa_samantha	Rh1AG372000 Rh1AG372200 Rh1AG372300 Rh1BG335100 Rh1BG335500 Rh1BG335700 Rh1BG335800 Rh1CG348600 Rh1CG349000 Rh1CG349100 Rh1CG349200 Rh1DG366500 Rh1DG366900 Rh1DG367000 Rh5BG537300 Rh5CG560800 Rh5DG548900
rosa_wichuraiana	Rw1G032720 Rw1G032730 Rw1G032740 Rw5G047750 Rw5G047880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	104, 1368
AccB1I	GGYRCC	1 cut(s)	27
AccB7I	CCANNNNNTGG	2 cut(s)	1591, 1804
AccI	GTMKAC	1 cut(s)	1818
AccII	CGCG	2 cut(s)	1496, 1761
AclWI	GGATC	2 cut(s)	1468, 2206
AcsI	RAATTY	5 cut(s)	197, 415, 1391, 1405, 2078
AcuI	CTGAAG	2 cut(s)	820, 2112
AfaI	GTAC	6 cut(s)	489, 1122, 1350, 1356, 1551, 1660
AfiI	CCNNNNNNNGG	6 cut(s)	589, 758, 1240, 1534, 1591, 1804
AflIII	ACRYGT	1 cut(s)	1060
AgsI	TTSAA	9 cut(s)	238, 461, 994, 1190, 1199, 1441, 1630, 1679, 1945
AhlI	ACTAGT	5 cut(s)	655, 667, 1075, 1357, 1672
AjnI	CCWGG	4 cut(s)	96, 250, 1584, 1690
AjuI	GAANNNNNNNTTGG	2 cut(s)	407, 439
AleI	CACNNNNGTG	1 cut(s)	1604
AloI	GAACNNNNNNTCC	2 cut(s)	214, 246
Alw26I	GTCTC	4 cut(s)	799, 977, 1511, 1874
AlwI	GGATC	2 cut(s)	1468, 2206
AoxI	GGCC	5 cut(s)	283, 390, 888, 1154, 1770
ApeKI	GCWGC	4 cut(s)	306, 938, 948, 1778
ApoI	RAATTY	5 cut(s)	197, 415, 1391, 1405, 2078
ArsI	GACNNNNNNTTYG	2 cut(s)	119, 151
Asp700I	GAANNNNTTC	2 cut(s)	804, 1405
AspLEI	GCGC	3 cut(s)	167, 1310, 1498
AspS9I	GGNCC	8 cut(s)	400, 716, 749, 1154, 1169, 1463, 1748, 1807
AsuC2I	CCSGG	3 cut(s)	934, 1001, 1885
AsuHPI	GGTGA	6 cut(s)	74, 1152, 1252, 1865, 2123, 2161
AsuII	TTCGAA	1 cut(s)	2209
AsuNHI	GCTAGC	1 cut(s)	298
AvaII	GGWCC	7 cut(s)	400, 716, 749, 1169, 1463, 1748, 1807
AxyI	CCTNAGG	1 cut(s)	280
BaeGI	GKGCMC	1 cut(s)	667
BaeI	ACNNNNGTAYC	2 cut(s)	458, 491
BanI	GGYRCC	1 cut(s)	27
BauI	CACGAG	1 cut(s)	18
BbsI	GAAGAC	1 cut(s)	1872
BbvCI	CCTCAGC	1 cut(s)	1919
BbvI	GCAGC	4 cut(s)	293, 925, 935, 1790
BccI	CCATC	8 cut(s)	32, 740, 899, 913, 1026, 1165, 1870, 1894
BceAI	ACGGC	3 cut(s)	15, 1512, 1954
BcgI	CGANNNNNNTGC	2 cut(s)	9, 43
BciT130I	CCWGG	4 cut(s)	98, 252, 1586, 1692
BciVI	GTATCC	4 cut(s)	284, 286, 1241, 1259
BclI	TGATCA	1 cut(s)	213
BcnI	CCSGG	3 cut(s)	934, 1001, 1885
BcoDI	GTCTC	4 cut(s)	799, 977, 1511, 1874
BcuI	ACTAGT	5 cut(s)	655, 667, 1075, 1357, 1672
BfaI	CTAG	8 cut(s)	299, 656, 668, 1076, 1271, 1358, 1532, 1673
BfmI	CTRYAG	1 cut(s)	189
BfoI	RGCGCY	1 cut(s)	168
BfuI	GTATCC	4 cut(s)	284, 286, 1241, 1259
BglI	GCCNNNNNGGC	1 cut(s)	1696
BisI	GCNGC	5 cut(s)	307, 939, 949, 1779, 2039
BlsI	GCNGC	5 cut(s)	308, 940, 950, 1780, 2040
Bme1390I	CCNGG	7 cut(s)	98, 252, 934, 1001, 1586, 1692, 1885
Bme18I	GGWCC	7 cut(s)	400, 716, 749, 1169, 1463, 1748, 1807
BmgT120I	GGNCC	8 cut(s)	400, 716, 749, 1154, 1169, 1463, 1748, 1807
BmiI	GGNNCC	2 cut(s)	29, 1155
BmrFI	CCNGG	7 cut(s)	98, 252, 934, 1001, 1586, 1692, 1885
BmrI	ACTGGG	1 cut(s)	1160
BmsI	GCATC	4 cut(s)	911, 1416, 1500, 1978
BmtI	GCTAGC	1 cut(s)	302
BmuI	ACTGGG	1 cut(s)	1160
BpiI	GAAGAC	1 cut(s)	1872
Bpu10I	CCTNAGC	2 cut(s)	1774, 1919
Bpu14I	TTCGAA	1 cut(s)	2209
BpuEI	CTTGAG	1 cut(s)	1441
BpuMI	CCSGG	3 cut(s)	934, 1001, 1885
Bsa29I	ATCGAT	1 cut(s)	1508
BsaAI	YACGTR	1 cut(s)	1784
BsaBI	GATNNNNATC	2 cut(s)	1542, 2016
BsaI	GGTCTC	3 cut(s)	799, 1511, 1874
BsaJI	CCNNGG	7 cut(s)	54, 251, 286, 444, 520, 933, 1000
BsaXI	ACNNNNNCTCC	4 cut(s)	1027, 1057, 1247, 1277
Bsc4I	CCNNNNNNNGG	6 cut(s)	589, 758, 1240, 1534, 1591, 1804
Bse1I	ACTGG	1 cut(s)	1155
Bse21I	CCTNAGG	1 cut(s)	280
Bse3DI	GCAATG	2 cut(s)	309, 881
Bse8I	GATNNNNATC	2 cut(s)	1542, 2016
BseBI	CCWGG	4 cut(s)	98, 252, 1586, 1692
BseCI	ATCGAT	1 cut(s)	1508
BseDI	CCNNGG	7 cut(s)	54, 251, 286, 444, 520, 933, 1000
BseGI	GGATG	4 cut(s)	1543, 1897, 1912, 2068
BseJI	GATNNNNATC	2 cut(s)	1542, 2016
BseLI	CCNNNNNNNGG	6 cut(s)	589, 758, 1240, 1534, 1591, 1804
BseMI	GCAATG	2 cut(s)	309, 881
BseMII	CTCAG	2 cut(s)	411, 1910
BseNI	ACTGG	1 cut(s)	1155
BseRI	GAGGAG	1 cut(s)	1886
BseSI	GKGCMC	1 cut(s)	667
BseXI	GCAGC	4 cut(s)	293, 925, 935, 1790
BseYI	CCCAGC	2 cut(s)	160, 2098
BsgI	GTGCAG	1 cut(s)	1661
Bsh1236I	CGCG	2 cut(s)	1496, 1761
Bsh1285I	CGRYCG	1 cut(s)	54
BshFI	GGCC	5 cut(s)	285, 392, 890, 1156, 1772
BshNI	GGYRCC	1 cut(s)	27
BshVI	ATCGAT	1 cut(s)	1508
BsiEI	CGRYCG	1 cut(s)	54
BsiSI	CCGG	4 cut(s)	389, 933, 1000, 1884
BslFI	GGGAC	4 cut(s)	122, 235, 1346, 1693
BslI	CCNNNNNNNGG	6 cut(s)	589, 758, 1240, 1534, 1591, 1804
BsmAI	GTCTC	4 cut(s)	799, 977, 1511, 1874
BsmFI	GGGAC	4 cut(s)	122, 235, 1346, 1693
BsnI	GGCC	5 cut(s)	285, 392, 890, 1156, 1772
Bso31I	GGTCTC	3 cut(s)	799, 1511, 1874
Bsp119I	TTCGAA	1 cut(s)	2209
Bsp1286I	GDGCHC	1 cut(s)	667
Bsp1407I	TGTACA	1 cut(s)	1658
Bsp143I	GATC	6 cut(s)	213, 1473, 1491, 2017, 2106, 2198
BspANI	GGCC	5 cut(s)	285, 392, 890, 1156, 1772
BspCNI	CTCAG	2 cut(s)	412, 1911
BspDI	ATCGAT	1 cut(s)	1508
BspFNI	CGCG	2 cut(s)	1496, 1761
BspHI	TCATGA	1 cut(s)	1573
BspLI	GGNNCC	2 cut(s)	29, 1155
BspMAI	CTGCAG	1 cut(s)	193
BspOI	GCTAGC	1 cut(s)	302
BspPI	GGATC	2 cut(s)	1468, 2206
BspT104I	TTCGAA	1 cut(s)	2209
BspT107I	GGYRCC	1 cut(s)	27
BspTNI	GGTCTC	3 cut(s)	799, 1511, 1874
BsrDI	GCAATG	2 cut(s)	309, 881
BsrGI	TGTACA	1 cut(s)	1658
BsrI	ACTGG	1 cut(s)	1155
BssECI	CCNNGG	7 cut(s)	54, 251, 286, 444, 520, 933, 1000
BssMI	GATC	6 cut(s)	213, 1473, 1491, 2017, 2106, 2198
BssNAI	GTATAC	1 cut(s)	1819
BssSI	CACGAG	1 cut(s)	18
Bst1107I	GTATAC	1 cut(s)	1819
Bst2BI	CACGAG	1 cut(s)	18
Bst2UI	CCWGG	4 cut(s)	98, 252, 1586, 1692
Bst4CI	ACNGT	6 cut(s)	268, 487, 852, 1663, 1816, 2051
Bst6I	CTCTTC	1 cut(s)	863
BstAUI	TGTACA	1 cut(s)	1658
BstBAI	YACGTR	1 cut(s)	1784
BstBI	TTCGAA	1 cut(s)	2209
BstC8I	GCNNGC	9 cut(s)	300, 304, 584, 888, 1297, 1344, 1431, 2043, 2121
BstDEI	CTNAG	7 cut(s)	280, 394, 420, 603, 1774, 1919, 2183
BstDSI	CCRYGG	1 cut(s)	520
BstF5I	GGATG	4 cut(s)	1543, 1897, 1912, 2068
BstFNI	CGCG	2 cut(s)	1496, 1761
BstH2I	RGCGCY	1 cut(s)	168
BstHHI	GCGC	3 cut(s)	167, 1310, 1498
BstKTI	GATC	6 cut(s)	216, 1476, 1494, 2020, 2109, 2201
BstMAI	GTCTC	4 cut(s)	799, 977, 1511, 1874
BstMBI	GATC	6 cut(s)	213, 1473, 1491, 2017, 2106, 2198
BstMCI	CGRYCG	1 cut(s)	54
BstMWI	GCNNNNNNNGC	5 cut(s)	74, 1305, 1339, 1696, 1778
BstNI	CCWGG	4 cut(s)	98, 252, 1586, 1692
BstNSI	RCATGY	3 cut(s)	134, 2045, 2119
BstSCI	CCNGG	7 cut(s)	96, 250, 932, 999, 1584, 1690, 1883
BstSFI	CTRYAG	1 cut(s)	189
BstSLI	GKGCMC	1 cut(s)	667
BstUI	CGCG	2 cut(s)	1496, 1761
BstV1I	GCAGC	4 cut(s)	293, 925, 935, 1790
BstV2I	GAAGAC	1 cut(s)	1872
BstZ17I	GTATAC	1 cut(s)	1819
Bsu15I	ATCGAT	1 cut(s)	1508
Bsu36I	CCTNAGG	1 cut(s)	280
BsuI	GTATCC	4 cut(s)	284, 286, 1241, 1259
BsuRI	GGCC	5 cut(s)	285, 392, 890, 1156, 1772
BsuTUI	ATCGAT	1 cut(s)	1508
BtgI	CCRYGG	1 cut(s)	520
BtsCI	GGATG	4 cut(s)	1543, 1897, 1912, 2068
BtsI	GCAGTG	1 cut(s)	552
BtsIMutI	CAGTG	1 cut(s)	552
Cac8I	GCNNGC	9 cut(s)	300, 304, 584, 888, 1297, 1344, 1431, 2043, 2121
CciI	TCATGA	1 cut(s)	1573
CfoI	GCGC	3 cut(s)	167, 1310, 1498
Cfr13I	GGNCC	8 cut(s)	400, 716, 749, 1154, 1169, 1463, 1748, 1807
ClaI	ATCGAT	1 cut(s)	1508
CseI	GACGC	2 cut(s)	123, 698
CsiI	ACCWGGT	2 cut(s)	96, 1584
Csp6I	GTAC	6 cut(s)	488, 1121, 1349, 1355, 1550, 1659
CspCI	CAANNNNNGTGG	2 cut(s)	903, 938
CviQI	GTAC	6 cut(s)	488, 1121, 1349, 1355, 1550, 1659
DdeI	CTNAG	7 cut(s)	280, 394, 420, 603, 1774, 1919, 2183
DpnI	GATC	6 cut(s)	215, 1475, 1493, 2019, 2108, 2200
DpnII	GATC	6 cut(s)	213, 1473, 1491, 2017, 2106, 2198
Eam1104I	CTCTTC	1 cut(s)	863
EarI	CTCTTC	1 cut(s)	863
EciI	GGCGGA	1 cut(s)	1761
Eco147I	AGGCCT	1 cut(s)	285
Eco31I	GGTCTC	3 cut(s)	799, 1511, 1874
Eco32I	GATATC	1 cut(s)	1957
Eco47I	GGWCC	7 cut(s)	400, 716, 749, 1169, 1463, 1748, 1807
Eco57I	CTGAAG	2 cut(s)	820, 2112
Eco81I	CCTNAGG	1 cut(s)	280
EcoO109I	RGGNCCY	1 cut(s)	400
EcoRI	GAATTC	1 cut(s)	415
EcoRII	CCWGG	4 cut(s)	96, 250, 1584, 1690
EcoRV	GATATC	1 cut(s)	1957
EcoT22I	ATGCAT	1 cut(s)	1515
FaqI	GGGAC	4 cut(s)	122, 235, 1346, 1693
FauI	CCCGC	3 cut(s)	303, 591, 1335
FauNDI	CATATG	2 cut(s)	907, 1422
FbaI	TGATCA	1 cut(s)	213
FblI	GTMKAC	1 cut(s)	1818
Fnu4HI	GCNGC	5 cut(s)	307, 939, 949, 1779, 2039
FokI	GGATG	4 cut(s)	1550, 1904, 1919, 2075
Fsp4HI	GCNGC	5 cut(s)	307, 939, 949, 1779, 2039
FspBI	CTAG	8 cut(s)	299, 656, 668, 1076, 1271, 1358, 1532, 1673
GlaI	GCGC	3 cut(s)	166, 1309, 1497
GluI	GCNGC	5 cut(s)	307, 939, 949, 1779, 2039
GsaI	CCCAGC	2 cut(s)	164, 2102
HaeII	RGCGCY	1 cut(s)	168
HaeIII	GGCC	5 cut(s)	285, 392, 890, 1156, 1772
HapII	CCGG	4 cut(s)	389, 933, 1000, 1884
HgaI	GACGC	2 cut(s)	123, 698
HhaI	GCGC	3 cut(s)	167, 1310, 1498
Hin6I	GCGC	3 cut(s)	165, 1308, 1496
HinP1I	GCGC	3 cut(s)	165, 1308, 1496
HincII	GTYRAC	1 cut(s)	1597
HindII	GTYRAC	1 cut(s)	1597
HinfI	GANTC	4 cut(s)	318, 330, 1895, 1970
HpaII	CCGG	4 cut(s)	389, 933, 1000, 1884
HphI	GGTGA	6 cut(s)	74, 1152, 1252, 1865, 2123, 2161
Hpy166II	GTNNAC	5 cut(s)	1172, 1463, 1597, 1659, 1819
Hpy188I	TCNGA	8 cut(s)	126, 290, 421, 688, 799, 1450, 2077, 2131
Hpy188III	TCNNGA	4 cut(s)	838, 1029, 1471, 1574
Hpy8I	GTNNAC	5 cut(s)	1172, 1463, 1597, 1659, 1819
Hpy99I	CGWCG	2 cut(s)	139, 692
HpyAV	CCTTC	3 cut(s)	611, 1372, 2028
HpyCH4III	ACNGT	6 cut(s)	268, 487, 852, 1663, 1816, 2051
HpyCH4IV	ACGT	3 cut(s)	1062, 1605, 1783
HpyF10VI	GCNNNNNNNGC	5 cut(s)	74, 1305, 1339, 1696, 1778
HpyF3I	CTNAG	7 cut(s)	280, 394, 420, 603, 1774, 1919, 2183
HpySE526I	ACGT	3 cut(s)	1062, 1605, 1783
HspAI	GCGC	3 cut(s)	165, 1308, 1496
Ksp22I	TGATCA	1 cut(s)	213
Kzo9I	GATC	6 cut(s)	213, 1473, 1491, 2017, 2106, 2198
LmnI	GCTCC	4 cut(s)	391, 524, 1053, 2095
Lsp1109I	GCAGC	4 cut(s)	293, 925, 935, 1790
LweI	GCATC	4 cut(s)	911, 1416, 1500, 1978
MabI	ACCWGGT	2 cut(s)	96, 1584
MaeI	CTAG	8 cut(s)	299, 656, 668, 1076, 1271, 1358, 1532, 1673
MaeII	ACGT	3 cut(s)	1062, 1605, 1783
MaeIII	GTNAC	7 cut(s)	32, 92, 772, 928, 1606, 1853, 2111
MalI	GATC	6 cut(s)	215, 1475, 1493, 2019, 2108, 2200
MboI	GATC	6 cut(s)	213, 1473, 1491, 2017, 2106, 2198
MfeI	CAATTG	1 cut(s)	597
MhlI	GDGCHC	1 cut(s)	667
MlyI	GAGTC	2 cut(s)	1904, 1964
MmeI	TCCRAC	3 cut(s)	711, 1645, 2011
Mph1103I	ATGCAT	1 cut(s)	1515
MroXI	GAANNNNTTC	2 cut(s)	804, 1405
MseI	TTAA	4 cut(s)	120, 1145, 1844, 1914
MslI	CAYNNNNRTG	2 cut(s)	209, 1604
MspA1I	CMGCKG	1 cut(s)	306
MspI	CCGG	4 cut(s)	389, 933, 1000, 1884
MspR9I	CCNGG	7 cut(s)	98, 252, 934, 1001, 1586, 1692, 1885
MunI	CAATTG	1 cut(s)	597
MvaI	CCWGG	4 cut(s)	98, 252, 1586, 1692
MvnI	CGCG	2 cut(s)	1496, 1761
MwoI	GCNNNNNNNGC	5 cut(s)	74, 1305, 1339, 1696, 1778
NciI	CCSGG	3 cut(s)	934, 1001, 1885
NdeI	CATATG	2 cut(s)	907, 1422
NdeII	GATC	6 cut(s)	213, 1473, 1491, 2017, 2106, 2198
NheI	GCTAGC	1 cut(s)	298
NlaIV	GGNNCC	2 cut(s)	29, 1155
NmeAIII	GCCGAG	2 cut(s)	425, 960
NmuCI	GTSAC	3 cut(s)	1606, 1853, 2111
NsiI	ATGCAT	1 cut(s)	1515
NspI	RCATGY	3 cut(s)	134, 2045, 2119
NspV	TTCGAA	1 cut(s)	2209
OliI	CACNNNNGTG	1 cut(s)	1604
PaeI	GCATGC	1 cut(s)	2045
PagI	TCATGA	1 cut(s)	1573
PceI	AGGCCT	1 cut(s)	285
PdmI	GAANNNNTTC	2 cut(s)	804, 1405
PfeI	GAWTC	2 cut(s)	318, 330
PflFI	GACNNNGTC	1 cut(s)	1705
PflMI	CCANNNNNTGG	2 cut(s)	1591, 1804
PkrI	GCNGC	5 cut(s)	308, 940, 950, 1780, 2040
PleI	GAGTC	2 cut(s)	1903, 1964
PpsI	GAGTC	2 cut(s)	1903, 1964
Ppu21I	YACGTR	1 cut(s)	1784
PpuMI	RGGWCCY	1 cut(s)	400
PsiI	TTATAA	2 cut(s)	104, 1368
Psp5II	RGGWCCY	1 cut(s)	400
Psp6I	CCWGG	4 cut(s)	96, 250, 1584, 1690
PspFI	CCCAGC	2 cut(s)	160, 2098
PspGI	CCWGG	4 cut(s)	96, 250, 1584, 1690
PspN4I	GGNNCC	2 cut(s)	29, 1155
PspPI	GGNCC	8 cut(s)	400, 716, 749, 1154, 1169, 1463, 1748, 1807
PspPPI	RGGWCCY	1 cut(s)	400
PstI	CTGCAG	1 cut(s)	193
PsyI	GACNNNGTC	1 cut(s)	1705
PvuII	CAGCTG	1 cut(s)	306
RsaI	GTAC	6 cut(s)	489, 1122, 1350, 1356, 1551, 1660
RsaNI	GTAC	6 cut(s)	488, 1121, 1349, 1355, 1550, 1659
RseI	CAYNNNNRTG	2 cut(s)	209, 1604
SaqAI	TTAA	4 cut(s)	120, 1145, 1844, 1914
SatI	GCNGC	5 cut(s)	307, 939, 949, 1779, 2039
Sau3AI	GATC	6 cut(s)	213, 1473, 1491, 2017, 2106, 2198
Sau96I	GGNCC	8 cut(s)	400, 716, 749, 1154, 1169, 1463, 1748, 1807
SchI	GAGTC	2 cut(s)	1904, 1964
ScrFI	CCNGG	7 cut(s)	98, 252, 934, 1001, 1586, 1692, 1885
SduI	GDGCHC	1 cut(s)	667
SexAI	ACCWGGT	2 cut(s)	96, 1584
SfaNI	GCATC	4 cut(s)	911, 1416, 1500, 1978
SfcI	CTRYAG	1 cut(s)	189
SfuI	TTCGAA	1 cut(s)	2209
SinI	GGWCC	7 cut(s)	400, 716, 749, 1169, 1463, 1748, 1807
SmiMI	CAYNNNNRTG	2 cut(s)	209, 1604
SmlI	CTYRAG	1 cut(s)	1456
SmoI	CTYRAG	1 cut(s)	1456
SpeI	ACTAGT	5 cut(s)	655, 667, 1075, 1357, 1672
SphI	GCATGC	1 cut(s)	2045
SseBI	AGGCCT	1 cut(s)	285
SspI	AATATT	2 cut(s)	990, 1649
SspMI	CTAG	8 cut(s)	299, 656, 668, 1076, 1271, 1358, 1532, 1673
StuI	AGGCCT	1 cut(s)	285
StyD4I	CCNGG	7 cut(s)	96, 250, 932, 999, 1584, 1690, 1883
TaaI	ACNGT	6 cut(s)	268, 487, 852, 1663, 1816, 2051
TaiI	ACGT	3 cut(s)	1065, 1608, 1786
TaqI	TCGA	7 cut(s)	854, 1030, 1215, 1508, 1898, 1959, 2209
TaqII	GACCGA	1 cut(s)	68
TatI	WGTACW	3 cut(s)	487, 1354, 1658
TauI	GCSGC	1 cut(s)	2041
TfiI	GAWTC	2 cut(s)	318, 330
Tru1I	TTAA	4 cut(s)	120, 1145, 1844, 1914
Tru9I	TTAA	4 cut(s)	120, 1145, 1844, 1914
TscAI	CASTG	1 cut(s)	552
TseFI	GTSAC	3 cut(s)	1606, 1853, 2111
TseI	GCWGC	4 cut(s)	306, 938, 948, 1778
Tsp45I	GTSAC	3 cut(s)	1606, 1853, 2111
TspGWI	ACGGA	1 cut(s)	537
TspRI	CASTG	1 cut(s)	552
Tth111I	GACNNNGTC	1 cut(s)	1705
Van91I	CCANNNNNTGG	2 cut(s)	1591, 1804
VpaK11BI	GGWCC	7 cut(s)	400, 716, 749, 1169, 1463, 1748, 1807
XapI	RAATTY	5 cut(s)	197, 415, 1391, 1405, 2078
XceI	RCATGY	3 cut(s)	134, 2045, 2119
XmiI	GTMKAC	1 cut(s)	1818
XmnI	GAANNNNTTC	2 cut(s)	804, 1405
XspI	CTAG	8 cut(s)	299, 656, 668, 1076, 1271, 1358, 1532, 1673
Zsp2I	ATGCAT	1 cut(s)	1515

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.