FvH4_7g30390

DNA-directed primase polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001804
Physical Location & Seq
Forward (+)
22145571 .. 22149864
4294 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g30390.t7

Sequence Viewer

Length: 1752 bp
ATGGACGACGTTGATCGCTTGTTCGAGTGCTTCAAGTGCGGCATCTCTCCTCCACAATCTGCTTTGAGAGAGAGAAAGAGAATCAAGAGCAAACCGAATCAGAGGAGTAAGGCCCAGGAAGTTGATTCTCCGCGGTCGCCGGAGCAAATAAAGAAGATTGCATCAGATTTACAGCTTTCTGCAGAAAAAGGTGGTTCTGCAACAGCTGAAGCGAAAAGCGTTAATCGTAGGAAGCAGTTATCTCCGGTTGTGTTTTACGGGTCTCCAAATGGAGTGCCTCCAAAGCAACCATCAAGGCTGTTGCTGCGTGTGTTGCGTGAGATACGTGTTGATCTCACTGCGCAAAAACGATTGAGTTCAAGGAAGGAAGTATGGGCTACATTTCCGAGGCAGGAGGAAGCAATGCAGTTTGCCAAACGGAACGAAAATGTTCATGTTTTTAGTTATCAAGATCACTTTAGTGGACAAAGAAGGTTCCTTGTTTCAAGTTATGACGAGTTTTGGAGAAGATACAAATTTATGGATTCCAAGTTTCGTCACCATTACGAAGTCATTCAGGAGGGTTTACCATGCCACCTTTATTTTGACCTGGAATTCAATAAGAAAGACAATACAGACAGAAATGGAGATGAAATGGTTGATCTGTTAATATCAGTTGTTTTCGAAGCGTTGCTTGAAAAGTATTCTATTCAAGGGAACATGGAGTGGATAATTGAGCTTGATTCCTCTACGGAAGCAAAGTTCTCTCGACATCTAATTGTTCGTATCCAAAAGACTGCTTTTAAGGACAACTCACATATAGGTGGATTTGTCACTGAGATATGCTCACGGATATCGAGTGCAAAGGGGAGGGATGGAAGATTTGAGAAATTGTTTATTACAAAAGATTCAAGCTCTGGAGATTTGGAGCCTAGTCACCTATTTGTGGACACTGCTGTGTATACAAGGAATCGTTGTTTTCGTCTAGCTCTATCATCAAAGGCAGGGAAGAAGTCGGTGCTTCTACCTACTGGGCGCTTCAAAGCTAAGGACATGATGCAGTCTGAAGAGGAGATGTTCATGGCGTCGTTGATCTGCAATTTGGATGCTGATTGTGAGAAGCTTCTAGTCTGCAAGTCTGATCTTGATTGTATAAAGACCCTGCATTTTGATACAGAGGTAAACCCTAGTTTTGGAAAATCTTACAGTTGGCCCCAAGAATTTGCACTGAGTGGTTGCACCAGTGATGCTTCAGCAACATACTTCATGGGGAAATCTCCATTTCCAGCTTTGGATGCATTTGTGGAATTTGTTGCTACAATTGGAAATGTATCAGGTAAAATTCGCAGCTGGTACTGGTTCTCAGAGTTTGGACTCATGGTCTACAGTATGTCGAGAAACAGATACTGTGAGCGAATCGGCAGACAGCACAAAAGCAATCATGTGATATACATTGCTGACCTCAGAAGGGGTGTTTATTATCAGAAATGTCATGATCCTGATTGCAGAGGTTATCGTTCTCCATCACGTCCAATTCCGTGGAATATTGTCCGTGACCTGGTGCATGAATGTGAAAAAGATCATGAACGTGTTCTTTTTGATGATGAGAACACGAAAAGCAGTGGTGTTACAGATTCATGGTGGCTTGAAGCCATAAAAGTTGCAGATGAAGTTGAAAATAAGCAAAATTCATTAGAGCTCAGCAATCCGAAAACCTTGATCCAGAAGATGACGATGACTGGTGGACTGCTGCTGCATCCCAAGTTGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Herpes_UL52

Protein Analysis

584

Amino Acids

67.2

Weight (kDa)

8.79

Isoelectric Point (pI)

49.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Herpes_UL52 PF03121 449 - 510 2e-12 Herpesviridae UL52/UL70 DNA primase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 342
AccI GTMKAC 2 cut(s) 941, 1362
AccII CGCG 1 cut(s) 133
AciI CCGC 3 cut(s) 39, 131, 133
AclWI GGATC 2 cut(s) 1469, 1693
AcsI RAATTY 6 cut(s) 515, 593, 1199, 1286, 1320, 1666
AcuI CTGAAG 3 cut(s) 228, 1065, 1215
AcyI GRCGYC 1 cut(s) 1064
AdeI CACNNNGTG 1 cut(s) 1211
AfaI GTAC 1 cut(s) 1334
AfiI CCNNNNNNNGG 4 cut(s) 925, 1172, 1447, 1537
AflIII ACRYGT 2 cut(s) 325, 1567
AhdI GACNNNNNGTC 1 cut(s) 1358
AjiI CACGTC 1 cut(s) 1508
AjnI CCWGG 3 cut(s) 114, 588, 1536
AleI CACNNNNGTG 2 cut(s) 459, 935
AloI GAACNNNNNNTCC 2 cut(s) 725, 757
Alw21I GWGCWC 1 cut(s) 1680
Alw26I GTCTC 1 cut(s) 267
AlwI GGATC 2 cut(s) 1469, 1693
AlwNI CAGNNNCTG 1 cut(s) 1386
AoxI GGCC 2 cut(s) 111, 1190
ApeKI GCWGC 4 cut(s) 304, 1326, 1729, 1732
ApoI RAATTY 6 cut(s) 515, 593, 1199, 1286, 1320, 1666
Asp700I GAANNNNTTC 3 cut(s) 429, 552, 1569
AspLEI GCGC 2 cut(s) 343, 1017
AspS9I GGNCC 2 cut(s) 112, 1191
AsuHPI GGTGA 2 cut(s) 530, 908
AsuII TTCGAA 1 cut(s) 663
BaeI ACNNNNGTAYC 2 cut(s) 1143, 1176
BanII GRGCYC 1 cut(s) 1680
Bbv12I GWGCWC 1 cut(s) 1680
BbvI GCAGC 4 cut(s) 291, 1338, 1716, 1719
BccI CCATC 3 cut(s) 298, 848, 1510
BciT130I CCWGG 3 cut(s) 116, 590, 1538
BciVI GTATCC 1 cut(s) 776
BcoDI GTCTC 1 cut(s) 267
BfaI CTAG 4 cut(s) 912, 965, 1106, 1167
BfmI CTRYAG 2 cut(s) 180, 1363
BfoI RGCGCY 1 cut(s) 1018
BfuI GTATCC 1 cut(s) 776
BisI GCNGC 5 cut(s) 40, 305, 1327, 1730, 1733
BlpI GCTNAGC 1 cut(s) 1679
BlsI GCNGC 5 cut(s) 41, 306, 1328, 1731, 1734
Bme1390I CCNGG 3 cut(s) 116, 590, 1538
BmeRI GACNNNNNGTC 1 cut(s) 1358
BmgBI CACGTC 1 cut(s) 1508
BmgT120I GGNCC 2 cut(s) 112, 1191
BmiI GGNNCC 3 cut(s) 476, 909, 1193
BmrFI CCNGG 3 cut(s) 116, 590, 1538
BmrI ACTGGG 1 cut(s) 1020
BmsI GCATC 7 cut(s) 51, 170, 1026, 1075, 1216, 1264, 1744
BmuI ACTGGG 1 cut(s) 1020
BplI GAGNNNNNCTC 2 cut(s) 809, 841
BpmI CTGGAG 1 cut(s) 918
Bpu10I CCTNAGC 1 cut(s) 1026
Bpu1102I GCTNAGC 1 cut(s) 1679
Bpu14I TTCGAA 1 cut(s) 663
BsaAI YACGTR 1 cut(s) 326
BsaHI GRCGYC 1 cut(s) 1064
BsaI GGTCTC 1 cut(s) 267
BsaJI CCNNGG 4 cut(s) 114, 131, 386, 1517
BsaWI WCCGGW 1 cut(s) 244
Bsc4I CCNNNNNNNGG 4 cut(s) 925, 1172, 1447, 1537
Bse1I ACTGG 4 cut(s) 1015, 1221, 1340, 1723
Bse3DI GCAATG 2 cut(s) 408, 1431
BseBI CCWGG 3 cut(s) 116, 590, 1538
BseDI CCNNGG 4 cut(s) 114, 131, 386, 1517
BseGI GGATG 4 cut(s) 859, 1090, 1279, 1735
BseLI CCNNNNNNNGG 4 cut(s) 925, 1172, 1447, 1537
BseMI GCAATG 2 cut(s) 408, 1431
BseMII CTCAG 5 cut(s) 807, 1199, 1356, 1456, 1693
BseNI ACTGG 4 cut(s) 1015, 1221, 1340, 1723
BseRI GAGGAG 3 cut(s) 39, 118, 1064
BseXI GCAGC 4 cut(s) 291, 1338, 1716, 1719
Bsh1236I CGCG 1 cut(s) 133
Bsh1285I CGRYCG 1 cut(s) 137
BshFI GGCC 2 cut(s) 113, 1192
BsiEI CGRYCG 1 cut(s) 137
BsiHKAI GWGCWC 1 cut(s) 1680
BsiSI CCGG 2 cut(s) 140, 245
BslI CCNNNNNNNGG 4 cut(s) 925, 1172, 1447, 1537
BsmAI GTCTC 1 cut(s) 267
BsnI GGCC 2 cut(s) 113, 1192
Bso31I GGTCTC 1 cut(s) 267
Bsp119I TTCGAA 1 cut(s) 663
Bsp1286I GDGCHC 1 cut(s) 1680
Bsp143I GATC 9 cut(s) 13, 331, 451, 640, 1071, 1120, 1474, 1558, 1698
Bsp1720I GCTNAGC 1 cut(s) 1679
BspACI CCGC 3 cut(s) 39, 131, 133
BspANI GGCC 2 cut(s) 113, 1192
BspCNI CTCAG 5 cut(s) 808, 1200, 1355, 1455, 1692
BspFNI CGCG 1 cut(s) 133
BspHI TCATGA 2 cut(s) 1471, 1561
BspLI GGNNCC 3 cut(s) 476, 909, 1193
BspMAI CTGCAG 1 cut(s) 184
BspPI GGATC 2 cut(s) 1469, 1693
BspT104I TTCGAA 1 cut(s) 663
BspTNI GGTCTC 1 cut(s) 267
BsrDI GCAATG 2 cut(s) 408, 1431
BsrI ACTGG 4 cut(s) 1015, 1221, 1340, 1723
BssECI CCNNGG 4 cut(s) 114, 131, 386, 1517
BssMI GATC 9 cut(s) 13, 331, 451, 640, 1071, 1120, 1474, 1558, 1698
BssNAI GTATAC 1 cut(s) 942
BssNI GRCGYC 1 cut(s) 1064
Bst1107I GTATAC 1 cut(s) 942
Bst2UI CCWGG 3 cut(s) 116, 590, 1538
Bst4CI ACNGT 3 cut(s) 1187, 1367, 1388
Bst6I CTCTTC 1 cut(s) 1041
BstACI GRCGYC 1 cut(s) 1064
BstBAI YACGTR 1 cut(s) 326
BstBI TTCGAA 1 cut(s) 663
BstDEI CTNAG 6 cut(s) 816, 1026, 1208, 1342, 1442, 1679
BstDSI CCRYGG 2 cut(s) 131, 1517
BstF5I GGATG 4 cut(s) 859, 1090, 1279, 1735
BstFNI CGCG 1 cut(s) 133
BstH2I RGCGCY 1 cut(s) 1018
BstHHI GCGC 2 cut(s) 343, 1017
BstKTI GATC 9 cut(s) 16, 334, 454, 643, 1074, 1123, 1477, 1561, 1701
BstMAI GTCTC 1 cut(s) 267
BstMBI GATC 9 cut(s) 13, 331, 451, 640, 1071, 1120, 1474, 1558, 1698
BstMCI CGRYCG 1 cut(s) 137
BstMWI GCNNNNNNNGC 5 cut(s) 36, 283, 304, 313, 1274
BstNI CCWGG 3 cut(s) 116, 590, 1538
BstSCI CCNGG 3 cut(s) 114, 588, 1536
BstSFI CTRYAG 2 cut(s) 180, 1363
BstUI CGCG 1 cut(s) 133
BstV1I GCAGC 4 cut(s) 291, 1338, 1716, 1719
BstXI CCANNNNNNTGG 1 cut(s) 1518
BstZ17I GTATAC 1 cut(s) 942
BsuI GTATCC 1 cut(s) 776
BsuRI GGCC 2 cut(s) 113, 1192
BtgI CCRYGG 2 cut(s) 131, 1517
BtrI CACGTC 1 cut(s) 1508
BtsCI GGATG 4 cut(s) 859, 1090, 1279, 1735
BtsI GCAGTG 3 cut(s) 336, 930, 1606
BtsIMutI CAGTG 6 cut(s) 336, 813, 930, 1205, 1228, 1606
CaiI CAGNNNCTG 1 cut(s) 1386
CciI TCATGA 2 cut(s) 1471, 1561
CfoI GCGC 2 cut(s) 343, 1017
Cfr13I GGNCC 2 cut(s) 112, 1191
Cfr42I CCGCGG 1 cut(s) 134
CseI GACGC 1 cut(s) 1053
CsiI ACCWGGT 1 cut(s) 1536
Csp6I GTAC 1 cut(s) 1333
CviQI GTAC 1 cut(s) 1333
DdeI CTNAG 6 cut(s) 816, 1026, 1208, 1342, 1442, 1679
DpnI GATC 9 cut(s) 15, 333, 453, 642, 1073, 1122, 1476, 1560, 1700
DpnII GATC 9 cut(s) 13, 331, 451, 640, 1071, 1120, 1474, 1558, 1698
DraIII CACNNNGTG 1 cut(s) 1211
DriI GACNNNNNGTC 1 cut(s) 1358
Eam1104I CTCTTC 1 cut(s) 1041
Eam1105I GACNNNNNGTC 1 cut(s) 1358
EarI CTCTTC 1 cut(s) 1041
Ecl136II GAGCTC 1 cut(s) 1678
Eco24I GRGCYC 1 cut(s) 1680
Eco31I GGTCTC 1 cut(s) 267
Eco32I GATATC 1 cut(s) 834
Eco53kI GAGCTC 1 cut(s) 1678
Eco57I CTGAAG 3 cut(s) 228, 1065, 1215
EcoICRI GAGCTC 1 cut(s) 1678
EcoRI GAATTC 1 cut(s) 593
EcoRII CCWGG 3 cut(s) 114, 588, 1536
EcoRV GATATC 1 cut(s) 834
EcoT22I ATGCAT 1 cut(s) 1279
EcoT38I GRGCYC 1 cut(s) 1680
FalI AAGNNNNNCTT 2 cut(s) 657, 689
FblI GTMKAC 2 cut(s) 941, 1362
Fnu4HI GCNGC 5 cut(s) 40, 305, 1327, 1730, 1733
FokI GGATG 4 cut(s) 866, 1097, 1286, 1722
FriOI GRGCYC 1 cut(s) 1680
Fsp4HI GCNGC 5 cut(s) 40, 305, 1327, 1730, 1733
FspBI CTAG 4 cut(s) 912, 965, 1106, 1167
FspI TGCGCA 1 cut(s) 342
GlaI GCGC 2 cut(s) 342, 1016
GluI GCNGC 5 cut(s) 40, 305, 1327, 1730, 1733
GsuI CTGGAG 1 cut(s) 918
HaeII RGCGCY 1 cut(s) 1018
HaeIII GGCC 2 cut(s) 113, 1192
HapII CCGG 2 cut(s) 140, 245
HgaI GACGC 1 cut(s) 1053
HhaI GCGC 2 cut(s) 343, 1017
Hin1I GRCGYC 1 cut(s) 1064
Hin6I GCGC 2 cut(s) 341, 1015
HinP1I GCGC 2 cut(s) 341, 1015
HindIII AAGCTT 1 cut(s) 1100
HpaII CCGG 2 cut(s) 140, 245
HphI GGTGA 2 cut(s) 530, 908
Hpy166II GTNNAC 7 cut(s) 464, 566, 928, 942, 1162, 1363, 1724
Hpy188I TCNGA 9 cut(s) 102, 166, 387, 1045, 1120, 1345, 1445, 1464, 1689
Hpy8I GTNNAC 7 cut(s) 464, 566, 928, 942, 1162, 1363, 1724
Hpy99I CGWCG 2 cut(s) 11, 1069
HpyAV CCTTC 3 cut(s) 358, 465, 1440
HpyCH4III ACNGT 3 cut(s) 1187, 1367, 1388
HpyCH4IV ACGT 4 cut(s) 9, 325, 1507, 1567
HpyF10VI GCNNNNNNNGC 5 cut(s) 36, 283, 304, 313, 1274
HpyF3I CTNAG 6 cut(s) 816, 1026, 1208, 1342, 1442, 1679
HpySE526I ACGT 4 cut(s) 9, 325, 1507, 1567
Hsp92I GRCGYC 1 cut(s) 1064
HspAI GCGC 2 cut(s) 341, 1015
KspI CCGCGG 1 cut(s) 134
Kzo9I GATC 9 cut(s) 13, 331, 451, 640, 1071, 1120, 1474, 1558, 1698
LmnI GCTCC 2 cut(s) 142, 907
Lsp1109I GCAGC 4 cut(s) 291, 1338, 1716, 1719
LweI GCATC 7 cut(s) 51, 170, 1026, 1075, 1216, 1264, 1744
MabI ACCWGGT 1 cut(s) 1536
MaeI CTAG 4 cut(s) 912, 965, 1106, 1167
MaeII ACGT 4 cut(s) 9, 325, 1507, 1567
MaeIII GTNAC 5 cut(s) 536, 811, 914, 1532, 1606
MalI GATC 9 cut(s) 15, 333, 453, 642, 1073, 1122, 1476, 1560, 1700
MboI GATC 9 cut(s) 13, 331, 451, 640, 1071, 1120, 1474, 1558, 1698
MboII GAAGA 6 cut(s) 166, 519, 870, 1000, 1058, 1717
MfeI CAATTG 1 cut(s) 1299
MhlI GDGCHC 1 cut(s) 1680
MlyI GAGTC 1 cut(s) 1347
Mph1103I ATGCAT 1 cut(s) 1279
MroXI GAANNNNTTC 3 cut(s) 429, 552, 1569
MseI TTAA 3 cut(s) 222, 647, 783
MslI CAYNNNNRTG 5 cut(s) 459, 801, 935, 1548, 1566
MspA1I CMGCKG 3 cut(s) 133, 206, 1329
MspI CCGG 2 cut(s) 140, 245
MspR9I CCNGG 3 cut(s) 116, 590, 1538
MunI CAATTG 1 cut(s) 1299
MvaI CCWGG 3 cut(s) 116, 590, 1538
MvnI CGCG 1 cut(s) 133
MwoI GCNNNNNNNGC 5 cut(s) 36, 283, 304, 313, 1274
NdeII GATC 9 cut(s) 13, 331, 451, 640, 1071, 1120, 1474, 1558, 1698
NlaIV GGNNCC 3 cut(s) 476, 909, 1193
NmuCI GTSAC 4 cut(s) 536, 811, 914, 1532
NsbI TGCGCA 1 cut(s) 342
NsiI ATGCAT 1 cut(s) 1279
NspV TTCGAA 1 cut(s) 663
OliI CACNNNNGTG 2 cut(s) 459, 935
PagI TCATGA 2 cut(s) 1471, 1561
PcsI WCGNNNNNNNCGW 1 cut(s) 958
PdmI GAANNNNTTC 3 cut(s) 429, 552, 1569
PfeI GAWTC 9 cut(s) 81, 97, 125, 524, 722, 887, 949, 1395, 1613
PkrI GCNGC 5 cut(s) 41, 306, 1328, 1731, 1734
PleI GAGTC 1 cut(s) 1347
PpsI GAGTC 1 cut(s) 1347
Ppu21I YACGTR 1 cut(s) 326
Psp124BI GAGCTC 1 cut(s) 1680
Psp6I CCWGG 3 cut(s) 114, 588, 1536
PspGI CCWGG 3 cut(s) 114, 588, 1536
PspN4I GGNNCC 3 cut(s) 476, 909, 1193
PspPI GGNCC 2 cut(s) 112, 1191
PstI CTGCAG 1 cut(s) 184
PstNI CAGNNNCTG 1 cut(s) 1386
PvuII CAGCTG 2 cut(s) 206, 1329
RsaI GTAC 1 cut(s) 1334
RsaNI GTAC 1 cut(s) 1333
RseI CAYNNNNRTG 5 cut(s) 459, 801, 935, 1548, 1566
SacI GAGCTC 1 cut(s) 1680
SacII CCGCGG 1 cut(s) 134
SaqAI TTAA 3 cut(s) 222, 647, 783
SatI GCNGC 5 cut(s) 40, 305, 1327, 1730, 1733
Sau3AI GATC 9 cut(s) 13, 331, 451, 640, 1071, 1120, 1474, 1558, 1698
Sau96I GGNCC 2 cut(s) 112, 1191
SchI GAGTC 1 cut(s) 1347
ScrFI CCNGG 3 cut(s) 116, 590, 1538
SduI GDGCHC 1 cut(s) 1680
SexAI ACCWGGT 1 cut(s) 1536
SfaNI GCATC 7 cut(s) 51, 170, 1026, 1075, 1216, 1264, 1744
SfcI CTRYAG 2 cut(s) 180, 1363
Sfr303I CCGCGG 1 cut(s) 134
SfuI TTCGAA 1 cut(s) 663
SgrBI CCGCGG 1 cut(s) 134
SmiMI CAYNNNNRTG 5 cut(s) 459, 801, 935, 1548, 1566
SsiI CCGC 3 cut(s) 39, 131, 133
SspI AATATT 1 cut(s) 1525
SspMI CTAG 4 cut(s) 912, 965, 1106, 1167
SstI GAGCTC 1 cut(s) 1680
StyD4I CCNGG 3 cut(s) 114, 588, 1536
TaaI ACNGT 3 cut(s) 1187, 1367, 1388
TaiI ACGT 4 cut(s) 12, 328, 1510, 1570
TaqI TCGA 5 cut(s) 24, 663, 748, 836, 1373
TauI GCSGC 1 cut(s) 42
TfiI GAWTC 9 cut(s) 81, 97, 125, 524, 722, 887, 949, 1395, 1613
Tru1I TTAA 3 cut(s) 222, 647, 783
Tru9I TTAA 3 cut(s) 222, 647, 783
TscAI CASTG 6 cut(s) 343, 820, 937, 1212, 1228, 1606
TseFI GTSAC 4 cut(s) 536, 811, 914, 1532
TseI GCWGC 4 cut(s) 304, 1326, 1729, 1732
Tsp45I GTSAC 4 cut(s) 536, 811, 914, 1532
TspDTI ATGAA 9 cut(s) 422, 645, 1048, 1234, 1560, 1578, 1605, 1659, 1662
TspGWI ACGGA 5 cut(s) 433, 746, 844, 1506, 1520
TspRI CASTG 6 cut(s) 343, 820, 937, 1212, 1228, 1606
XapI RAATTY 6 cut(s) 515, 593, 1199, 1286, 1320, 1666
XmiI GTMKAC 2 cut(s) 941, 1362
XmnI GAANNNNTTC 3 cut(s) 429, 552, 1569
XspI CTAG 4 cut(s) 912, 965, 1106, 1167
Zsp2I ATGCAT 1 cut(s) 1279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.