Rorug05G0124500

DNA-directed primase polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001804
Physical Location & Seq
Reverse (-)
10998162 .. 10998545
384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0124500.1

Sequence Viewer

Length: 384 bp
ATGCAATTCATACTGGTAGCGATACTGGTTCTTTTCACATTTGCGGCCGCGGCCGAAGAAGCAGTAGCAGCTGAAGCCGTCCTGCCTCAAGCCAAGCCTGGCTGCACTGACCGCTGTGGCAACCTCACCATTCCATATCCATTTGGCATAGGGCCTGATTGTTACATGACCAAAGACTTCTCTGTTCGTTGTAACACATCCACTAAACCCCCGACAGCAATATGGCGTGATAGTCCCATCGCTATTAACAACATATACCTGGCTGAAGGTGAGATGCAAATATCGCAGTACGTAGCCTACGATTGCTATGACAGCCAGGGCTGGACTAATTCCAGCGAACCATTGCTCGCCGTGCCTCCTTTAGATCACCATCTCTCATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Herpes_UL52

Protein Analysis

127

Amino Acids

13.92

Weight (kDa)

4.46

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 35 - 95 7.9e-11 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 50
AciI CCGC 4 cut(s) 44, 48, 50, 112
AcoI YGGCCR 2 cut(s) 45, 51
AcuI CTGAAG 2 cut(s) 93, 285
AfaI GTAC 1 cut(s) 290
AjnI CCWGG 3 cut(s) 97, 258, 315
AluBI AGCT 1 cut(s) 71
AluI AGCT 1 cut(s) 71
AoxI GGCC 3 cut(s) 45, 51, 152
ApeKI GCWGC 2 cut(s) 68, 102
AspS9I GGNCC 1 cut(s) 152
AsuHPI GGTGA 3 cut(s) 118, 281, 359
BbvI GCAGC 2 cut(s) 80, 89
BccI CCATC 2 cut(s) 245, 378
BceAI ACGGC 2 cut(s) 62, 335
BciT130I CCWGG 3 cut(s) 99, 260, 317
BfaI CTAG 1 cut(s) 382
BisI GCNGC 5 cut(s) 45, 48, 51, 69, 103
BlsI GCNGC 5 cut(s) 46, 49, 52, 70, 104
Bme1390I CCNGG 3 cut(s) 99, 260, 317
BmgT120I GGNCC 1 cut(s) 152
BmrFI CCNGG 3 cut(s) 99, 260, 317
BmsI GCATC 1 cut(s) 264
BpuEI CTTGAG 1 cut(s) 72
BsaAI YACGTR 1 cut(s) 292
BsaBI GATNNNNATC 1 cut(s) 369
BsaJI CCNNGG 2 cut(s) 48, 316
Bse1I ACTGG 2 cut(s) 18, 30
Bse3DI GCAATG 1 cut(s) 341
Bse8I GATNNNNATC 1 cut(s) 369
BseBI CCWGG 3 cut(s) 99, 260, 317
BseDI CCNNGG 2 cut(s) 48, 316
BseGI GGATG 1 cut(s) 197
BseJI GATNNNNATC 1 cut(s) 369
BseMI GCAATG 1 cut(s) 341
BseNI ACTGG 2 cut(s) 18, 30
BseX3I CGGCCG 2 cut(s) 45, 51
BseXI GCAGC 2 cut(s) 80, 89
BsgI GTGCAG 1 cut(s) 88
Bsh1236I CGCG 1 cut(s) 50
Bsh1285I CGRYCG 2 cut(s) 48, 54
BshFI GGCC 3 cut(s) 47, 53, 154
BsiEI CGRYCG 2 cut(s) 48, 54
BslFI GGGAC 1 cut(s) 219
BsmFI GGGAC 1 cut(s) 219
BsnI GGCC 3 cut(s) 47, 53, 154
Bsp143I GATC 1 cut(s) 364
BspACI CCGC 4 cut(s) 44, 48, 50, 112
BspANI GGCC 3 cut(s) 47, 53, 154
BspFNI CGCG 1 cut(s) 50
BsrDI GCAATG 1 cut(s) 341
BsrI ACTGG 2 cut(s) 18, 30
BssECI CCNNGG 2 cut(s) 48, 316
BssMI GATC 1 cut(s) 364
Bst2UI CCWGG 3 cut(s) 99, 260, 317
BstBAI YACGTR 1 cut(s) 292
BstC8I GCNNGC 1 cut(s) 348
BstDSI CCRYGG 1 cut(s) 48
BstF5I GGATG 1 cut(s) 197
BstFNI CGCG 1 cut(s) 50
BstKTI GATC 1 cut(s) 367
BstMBI GATC 1 cut(s) 364
BstMCI CGRYCG 2 cut(s) 48, 54
BstMWI GCNNNNNNNGC 8 cut(s) 50, 59, 68, 74, 111, 283, 312, 352
BstNI CCWGG 3 cut(s) 99, 260, 317
BstSCI CCNGG 3 cut(s) 97, 258, 315
BstSNI TACGTA 1 cut(s) 292
BstUI CGCG 1 cut(s) 50
BstV1I GCAGC 2 cut(s) 80, 89
BstZI CGGCCG 2 cut(s) 45, 51
BsuRI GGCC 3 cut(s) 47, 53, 154
BtgI CCRYGG 1 cut(s) 48
BtgZI GCGATG 1 cut(s) 223
BtsCI GGATG 1 cut(s) 197
BtsIMutI CAGTG 1 cut(s) 105
Cac8I GCNNGC 1 cut(s) 348
CciNI GCGGCCGC 1 cut(s) 45
Cfr13I GGNCC 1 cut(s) 152
Cfr42I CCGCGG 1 cut(s) 51
Csp6I GTAC 1 cut(s) 289
CviAII CATG 1 cut(s) 166
CviQI GTAC 1 cut(s) 289
DpnI GATC 1 cut(s) 366
DpnII GATC 1 cut(s) 364
EaeI YGGCCR 2 cut(s) 45, 51
EagI CGGCCG 2 cut(s) 45, 51
EclXI CGGCCG 2 cut(s) 45, 51
Eco105I TACGTA 1 cut(s) 292
Eco52I CGGCCG 2 cut(s) 45, 51
Eco57I CTGAAG 2 cut(s) 93, 285
EcoO109I RGGNCCY 1 cut(s) 152
EcoRII CCWGG 3 cut(s) 97, 258, 315
FaeI CATG 1 cut(s) 169
FaiI YATR 9 cut(s) 11, 136, 149, 167, 223, 254, 256, 309, 379
FaqI GGGAC 1 cut(s) 219
FatI CATG 1 cut(s) 165
Fnu4HI GCNGC 5 cut(s) 45, 48, 51, 69, 103
FokI GGATG 1 cut(s) 184
Fsp4HI GCNGC 5 cut(s) 45, 48, 51, 69, 103
FspBI CTAG 1 cut(s) 382
GluI GCNGC 5 cut(s) 45, 48, 51, 69, 103
HaeIII GGCC 3 cut(s) 47, 53, 154
Hin1II CATG 1 cut(s) 169
HphI GGTGA 3 cut(s) 118, 281, 359
HpyAV CCTTC 1 cut(s) 260
HpyCH4IV ACGT 1 cut(s) 291
HpyCH4V TGCA 3 cut(s) 4, 105, 277
HpyF10VI GCNNNNNNNGC 8 cut(s) 50, 59, 68, 74, 111, 283, 312, 352
HpySE526I ACGT 1 cut(s) 291
Hsp92II CATG 1 cut(s) 169
KspI CCGCGG 1 cut(s) 51
Kzo9I GATC 1 cut(s) 364
Lsp1109I GCAGC 2 cut(s) 80, 89
LweI GCATC 1 cut(s) 264
MaeI CTAG 1 cut(s) 382
MaeII ACGT 1 cut(s) 291
MaeIII GTNAC 2 cut(s) 161, 191
MalI GATC 1 cut(s) 366
MboI GATC 1 cut(s) 364
MboII GAAGA 1 cut(s) 68
MluCI AATT 2 cut(s) 5, 328
MnlI CCTC 3 cut(s) 96, 134, 366
MseI TTAA 1 cut(s) 246
MspA1I CMGCKG 3 cut(s) 50, 71, 114
MspR9I CCNGG 3 cut(s) 99, 260, 317
MvaI CCWGG 3 cut(s) 99, 260, 317
MvnI CGCG 1 cut(s) 50
MwoI GCNNNNNNNGC 8 cut(s) 50, 59, 68, 74, 111, 283, 312, 352
NdeII GATC 1 cut(s) 364
NlaIII CATG 1 cut(s) 169
NotI GCGGCCGC 1 cut(s) 45
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PkrI GCNGC 5 cut(s) 46, 49, 52, 70, 104
Ppu21I YACGTR 1 cut(s) 292
Psp6I CCWGG 3 cut(s) 97, 258, 315
PspGI CCWGG 3 cut(s) 97, 258, 315
PspPI GGNCC 1 cut(s) 152
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
SacII CCGCGG 1 cut(s) 51
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 5 cut(s) 45, 48, 51, 69, 103
Sau3AI GATC 1 cut(s) 364
Sau96I GGNCC 1 cut(s) 152
ScrFI CCNGG 3 cut(s) 99, 260, 317
SetI ASST 5 cut(s) 73, 126, 261, 271, 294
SfaNI GCATC 1 cut(s) 264
Sfr303I CCGCGG 1 cut(s) 51
SgrBI CCGCGG 1 cut(s) 51
SmlI CTYRAG 1 cut(s) 87
SmoI CTYRAG 1 cut(s) 87
SnaBI TACGTA 1 cut(s) 292
Sse9I AATT 2 cut(s) 5, 328
SsiI CCGC 4 cut(s) 44, 48, 50, 112
SspMI CTAG 1 cut(s) 382
StyD4I CCNGG 3 cut(s) 97, 258, 315
TaiI ACGT 1 cut(s) 294
TasI AATT 2 cut(s) 5, 328
TauI GCSGC 3 cut(s) 47, 50, 53
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TscAI CASTG 1 cut(s) 112
TseI GCWGC 2 cut(s) 68, 102
TspRI CASTG 1 cut(s) 112
XspI CTAG 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.