pycom01g22030

DNA-directed primase polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001804
Physical Location & Seq
Reverse (-)
19789196 .. 19792931
3736 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g22030.1

Sequence Viewer

Length: 1764 bp
ATGGACGACGTCGATCGGTTGTTCGAGTGCTTCAAGTGCGGCATCTCTCCTCCGCAATCCGCATTGAGGGAAAGGAAGAGAAGCAAAAGCGATTTGAAGCGGAGGAGTAAAACTCCGGAAGTCTCCTCTCCAGGCTCAGCAGAGAACACAAAGAAAAATACATCAGAATTACAACTTTCCTCCGACAAGTCTGGTCCTACAAGTGTTAAACCAAACAAGTTTAATCGTCAAAAGGAGTTTTCCCCAGTTGTATTCTATGGATCTCCACACGGTGTGCCCCCCAAAAAACCATCAAGGTCGTTTCTGCGACTGCTACGTGAAATACGTATTGATCTCGCTGCACAAAAAAGGTTGACTTCAAGGAAGGAAGTATGGACTACGTTTCCAAGGCAGGATGAAGCAATGCAGTTTGCCAAAGGTCATGAGAATGTTCATGTATTTAGTTATCAAGATCATTATAGTGGACAGAGAAGGTTCCTCGTTTCAACATATAATGATTTTTGGAGAAGGTATAAAAATATGAATCCCAAGTTCCGTCACCACTACGAAGTGATCCAGGAGGGTTTGCCATGTCACCTTTATTTTGACTTGGAATTCAGTAGAAGGGACAATGCAGACAGAAATGGAGATGAAATGGTTGACCTCTTGATATCAGCCATTTTTGAAGCCCTGCTTGAAAAGTATTCTATTATTGGAAACAAGGAATGGATATTGGAGCTTGATTCCTCTAATGAAGCAAAGTTCTCTCGTCACCTAATCATTCGCATAGAAAAGACGGCTTTTAAGGACAACTCACATGCAGGTGCATTTGTCACTGAGATATGCTCACGGATTTCAAGTGCAAAGGAGAGAGATGGAAGATTTGGAAACTTATTTATCAGAAAAGATTCAAGCTCCTCTGACTCCCCTAGTAAACTATTTGTGGACACTGCTGTATATACTCGAAATCGTTGCTTTCGTTTAGCTCTATCATCAAAGGCAGGGAAGAATTCAGTGCTTCTGCCTACTGGGCGTTTCAAAGCCAACAAGATGTGTGAGGAGGAGATGTTCATGGCATCCTTGATCTGCAATTTGGATGTTGATTGTGAAAAGCTTCTAGTATGCAAACCAGATCTTGATTGTATAAAGACTCTGCATTTTGACACCGAGGTGAACCGCGGTCTTGGAAAATGTTACAGTTGGCCCCAAGAATATGCATTGAATGGTTGCACAAGTGGTGCTTCAGCAACATACTTCCTGGGCAAATCTCCATTTCCAACTTTGGATGCATTTGTAGAATCTGTTGCCACCATTGGAAATGTATCAGGTAAAATTCGGAGCTGGTATTGGCTCTCAGAGTTTGCACTCATGGTCTACAGCATGTCAAGAAATAGATACTGTGAGCGAATTGGCAGACAGCATAAAAGCAATCACGTGATATACGTTGCTGACCTCAGAAGGGCTTCCTATTATCAGAAATGTCATGATCCTGACTGCAGAGGTTATCGTTCTGCCTCGCGACCAATTCCATGGGAAATCATCCCTGACATGGTGTATGAAAATAAAAATGATCCTGAATCTGTTCTCTATGGTGATGAGAAAATGAGAAGCAGCTGCGTTACAGATTCATGGTGGCGTGAAGCCATTAGAGTTGCAGATGATGTCGAGAATAAGCAAAATTCTTTAGGGATCAGTAATATGGAAAACATCGATTCCGATGATGAAGACTGGTGGATGGCTGTGGAAAGGACTGCATCCCAAGCTGAATTAGCGCATGTCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Herpes_UL52

Protein Analysis

588

Amino Acids

67.66

Weight (kDa)

8.61

Isoelectric Point (pI)

50.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Herpes_UL52 PF03121 452 - 504 3.1e-12 Herpesviridae UL52/UL70 DNA primase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 791
AatII GACGTC 1 cut(s) 12
Acc36I ACCTGC 1 cut(s) 791
AccI GTMKAC 1 cut(s) 1353
AccII CGCG 2 cut(s) 1158, 1498
AccIII TCCGGA 1 cut(s) 115
AciI CCGC 6 cut(s) 39, 53, 60, 100, 1156, 1158
AclWI GGATC 5 cut(s) 268, 547, 1460, 1544, 1676
AcsI RAATTY 4 cut(s) 593, 988, 1311, 1657
AcuI CTGAAG 1 cut(s) 1206
AcvI CACGTG 1 cut(s) 1414
AcyI GRCGYC 1 cut(s) 9
AdeI CACNNNGTG 2 cut(s) 272, 274
AfiI CCNNNNNNNGG 3 cut(s) 66, 1438, 1528
AjnI CCWGG 3 cut(s) 130, 555, 1236
AleI CACNNNNGTG 1 cut(s) 1148
AloI GAACNNNNNNTCC 2 cut(s) 1031, 1063
AluBI AGCT 7 cut(s) 718, 894, 965, 1093, 1320, 1593, 1742
AluI AGCT 7 cut(s) 718, 894, 965, 1093, 1320, 1593, 1742
Alw26I GTCTC 1 cut(s) 127
AlwI GGATC 5 cut(s) 268, 547, 1460, 1544, 1676
Aor13HI TCCGGA 1 cut(s) 115
AoxI GGCC 1 cut(s) 1181
ApeKI GCWGC 3 cut(s) 338, 1590, 1593
ApoI RAATTY 4 cut(s) 593, 988, 1311, 1657
Asp700I GAANNNNTTC 4 cut(s) 886, 1092, 1441, 1560
AspLEI GCGC 1 cut(s) 1753
AspS9I GGNCC 2 cut(s) 194, 1182
AsuHPI GGTGA 5 cut(s) 530, 566, 743, 1162, 1583
AvaII GGWCC 1 cut(s) 194
BaeGI GKGCMC 1 cut(s) 279
BbrPI CACGTG 1 cut(s) 1414
BbsI GAAGAC 1 cut(s) 1710
BbvI GCAGC 3 cut(s) 325, 1580, 1602
BccI CCATC 3 cut(s) 298, 848, 1708
BceAI ACGGC 1 cut(s) 792
BcgI CGANNNNNNTGC 2 cut(s) 933, 967
BciT130I CCWGG 3 cut(s) 132, 557, 1238
BcoDI GTCTC 1 cut(s) 127
BfaI CTAG 2 cut(s) 909, 1097
BfmI CTRYAG 2 cut(s) 1354, 1474
BfuAI ACCTGC 1 cut(s) 791
BglI GCCNNNNNGGC 1 cut(s) 1009
BglII AGATCT 1 cut(s) 1111
BisI GCNGC 4 cut(s) 40, 339, 1591, 1594
BlpI GCTNAGC 1 cut(s) 136
BlsI GCNGC 4 cut(s) 41, 340, 1592, 1595
Bme1390I CCNGG 3 cut(s) 132, 557, 1238
Bme18I GGWCC 1 cut(s) 194
BmgT120I GGNCC 2 cut(s) 194, 1182
BmiI GGNNCC 2 cut(s) 476, 1184
BmrFI CCNGG 3 cut(s) 132, 557, 1238
BmrI ACTGGG 2 cut(s) 239, 1017
BmsI GCATC 4 cut(s) 51, 1064, 1255, 1742
BmuI ACTGGG 2 cut(s) 239, 1017
BpiI GAAGAC 1 cut(s) 1710
BplI GAGNNNNNCTC 4 cut(s) 97, 129, 809, 841
BpmI CTGGAG 1 cut(s) 114
Bpu1102I GCTNAGC 1 cut(s) 136
Bsa29I ATCGAT 1 cut(s) 1689
BsaAI YACGTR 3 cut(s) 317, 326, 1414
BsaHI GRCGYC 1 cut(s) 9
BsaJI CCNNGG 5 cut(s) 386, 1146, 1156, 1237, 1508
BsaWI WCCGGW 1 cut(s) 115
BsaXI ACNNNNNCTCC 2 cut(s) 1031, 1061
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 1438, 1528
Bse1I ACTGG 3 cut(s) 245, 1012, 1712
Bse3DI GCAATG 1 cut(s) 408
BseAI TCCGGA 1 cut(s) 115
BseBI CCWGG 3 cut(s) 132, 557, 1238
BseCI ATCGAT 1 cut(s) 1689
BseDI CCNNGG 5 cut(s) 386, 1146, 1156, 1237, 1508
BseGI GGATG 7 cut(s) 400, 1055, 1081, 1270, 1518, 1719, 1733
BseLI CCNNNNNNNGG 3 cut(s) 66, 1438, 1528
BseMI GCAATG 1 cut(s) 408
BseMII CTCAG 4 cut(s) 150, 807, 1347, 1447
BseNI ACTGG 3 cut(s) 245, 1012, 1712
BseRI GAGGAG 6 cut(s) 39, 115, 118, 886, 1052, 1055
BseSI GKGCMC 1 cut(s) 279
BseXI GCAGC 3 cut(s) 325, 1580, 1602
BsgI GTGCAG 1 cut(s) 324
Bsh1236I CGCG 2 cut(s) 1158, 1498
Bsh1285I CGRYCG 1 cut(s) 16
BshFI GGCC 1 cut(s) 1183
BshVI ATCGAT 1 cut(s) 1689
BsiEI CGRYCG 1 cut(s) 16
BsiSI CCGG 1 cut(s) 116
BslFI GGGAC 1 cut(s) 620
BslI CCNNNNNNNGG 3 cut(s) 66, 1438, 1528
BsmAI GTCTC 1 cut(s) 127
BsmFI GGGAC 1 cut(s) 620
BsnI GGCC 1 cut(s) 1183
Bsp1286I GDGCHC 1 cut(s) 279
Bsp13I TCCGGA 1 cut(s) 115
Bsp1720I GCTNAGC 1 cut(s) 136
Bsp19I CCATGG 1 cut(s) 1508
Bsp68I TCGCGA 1 cut(s) 1498
BspACI CCGC 6 cut(s) 39, 53, 60, 100, 1156, 1158
BspANI GGCC 1 cut(s) 1183
BspCNI CTCAG 4 cut(s) 149, 808, 1346, 1446
BspDI ATCGAT 1 cut(s) 1689
BspEI TCCGGA 1 cut(s) 115
BspFNI CGCG 2 cut(s) 1158, 1498
BspHI TCATGA 2 cut(s) 421, 1462
BspLI GGNNCC 2 cut(s) 476, 1184
BspMAI CTGCAG 1 cut(s) 1478
BspMI ACCTGC 1 cut(s) 791
BspPI GGATC 5 cut(s) 268, 547, 1460, 1544, 1676
BsrDI GCAATG 1 cut(s) 408
BsrI ACTGG 3 cut(s) 245, 1012, 1712
BssECI CCNNGG 5 cut(s) 386, 1146, 1156, 1237, 1508
BssNI GRCGYC 1 cut(s) 9
BssT1I CCWWGG 2 cut(s) 386, 1508
Bst2UI CCWGG 3 cut(s) 132, 557, 1238
Bst4CI ACNGT 3 cut(s) 272, 1178, 1379
Bst6I CTCTTC 1 cut(s) 71
BstACI GRCGYC 1 cut(s) 9
BstBAI YACGTR 3 cut(s) 317, 326, 1414
BstDEI CTNAG 4 cut(s) 136, 816, 1333, 1433
BstDSI CCRYGG 2 cut(s) 1156, 1508
BstF5I GGATG 7 cut(s) 400, 1055, 1081, 1270, 1518, 1719, 1733
BstFNI CGCG 2 cut(s) 1158, 1498
BstHHI GCGC 1 cut(s) 1753
BstMAI GTCTC 1 cut(s) 127
BstMCI CGRYCG 1 cut(s) 16
BstMWI GCNNNNNNNGC 4 cut(s) 36, 1009, 1739, 1748
BstNI CCWGG 3 cut(s) 132, 557, 1238
BstNSI RCATGY 3 cut(s) 800, 1363, 1757
BstSCI CCNGG 3 cut(s) 130, 555, 1236
BstSFI CTRYAG 2 cut(s) 1354, 1474
BstSLI GKGCMC 1 cut(s) 279
BstSNI TACGTA 1 cut(s) 326
BstUI CGCG 2 cut(s) 1158, 1498
BstV1I GCAGC 3 cut(s) 325, 1580, 1602
BstV2I GAAGAC 1 cut(s) 1710
BstX2I RGATCY 2 cut(s) 260, 1111
BstXI CCANNNNNNTGG 1 cut(s) 1509
BstYI RGATCY 2 cut(s) 260, 1111
Bsu15I ATCGAT 1 cut(s) 1689
BsuRI GGCC 1 cut(s) 1183
BsuTUI ATCGAT 1 cut(s) 1689
BtgI CCRYGG 2 cut(s) 1156, 1508
BtsCI GGATG 7 cut(s) 400, 1055, 1081, 1270, 1518, 1719, 1733
BtsI GCAGTG 1 cut(s) 927
BtsIMutI CAGTG 3 cut(s) 813, 927, 999
BtuMI TCGCGA 1 cut(s) 1498
BveI ACCTGC 1 cut(s) 791
CciI TCATGA 2 cut(s) 421, 1462
CfoI GCGC 1 cut(s) 1753
Cfr13I GGNCC 2 cut(s) 194, 1182
Cfr42I CCGCGG 1 cut(s) 1159
ClaI ATCGAT 1 cut(s) 1689
DdeI CTNAG 4 cut(s) 136, 816, 1333, 1433
DraIII CACNNNGTG 2 cut(s) 272, 274
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
Eco105I TACGTA 1 cut(s) 326
Eco130I CCWWGG 2 cut(s) 386, 1508
Eco32I GATATC 1 cut(s) 651
Eco47I GGWCC 1 cut(s) 194
Eco57I CTGAAG 1 cut(s) 1206
Eco72I CACGTG 1 cut(s) 1414
EcoRI GAATTC 2 cut(s) 593, 988
EcoRII CCWGG 3 cut(s) 130, 555, 1236
EcoRV GATATC 1 cut(s) 651
EcoT14I CCWWGG 2 cut(s) 386, 1508
EcoT22I ATGCAT 2 cut(s) 1198, 1270
ErhI CCWWGG 2 cut(s) 386, 1508
FalI AAGNNNNNCTT 6 cut(s) 340, 372, 657, 689, 1204, 1236
FaqI GGGAC 1 cut(s) 620
FblI GTMKAC 1 cut(s) 1353
Fnu4HI GCNGC 4 cut(s) 40, 339, 1591, 1594
FokI GGATG 7 cut(s) 407, 1042, 1088, 1277, 1505, 1720, 1726
Fsp4HI GCNGC 4 cut(s) 40, 339, 1591, 1594
FspBI CTAG 2 cut(s) 909, 1097
GlaI GCGC 1 cut(s) 1752
GluI GCNGC 4 cut(s) 40, 339, 1591, 1594
GsuI CTGGAG 1 cut(s) 114
HaeIII GGCC 1 cut(s) 1183
HapII CCGG 1 cut(s) 116
HhaI GCGC 1 cut(s) 1753
Hin1I GRCGYC 1 cut(s) 9
Hin6I GCGC 1 cut(s) 1751
HinP1I GCGC 1 cut(s) 1751
HincII GTYRAC 3 cut(s) 354, 640, 1759
HindII GTYRAC 3 cut(s) 354, 640, 1759
HindIII AAGCTT 1 cut(s) 1091
HinfI GANTC 9 cut(s) 523, 722, 887, 902, 1129, 1277, 1556, 1604, 1691
HpaII CCGG 1 cut(s) 116
HphI GGTGA 5 cut(s) 530, 566, 743, 1162, 1583
Hpy166II GTNNAC 8 cut(s) 354, 464, 640, 914, 925, 1153, 1354, 1759
Hpy188I TCNGA 9 cut(s) 166, 184, 881, 901, 1317, 1336, 1436, 1455, 1696
Hpy8I GTNNAC 8 cut(s) 354, 464, 640, 914, 925, 1153, 1354, 1759
Hpy99I CGWCG 2 cut(s) 11, 14
HpyAV CCTTC 5 cut(s) 358, 465, 501, 597, 1431
HpyCH4III ACNGT 3 cut(s) 272, 1178, 1379
HpyCH4IV ACGT 6 cut(s) 9, 316, 325, 380, 1413, 1422
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 1009, 1739, 1748
HpyF3I CTNAG 4 cut(s) 136, 816, 1333, 1433
HpySE526I ACGT 6 cut(s) 9, 316, 325, 380, 1413, 1422
Hsp92I GRCGYC 1 cut(s) 9
HspAI GCGC 1 cut(s) 1751
Kpn2I TCCGGA 1 cut(s) 115
KspI CCGCGG 1 cut(s) 1159
LmnI GCTCC 3 cut(s) 715, 899, 1317
Lsp1109I GCAGC 3 cut(s) 325, 1580, 1602
LweI GCATC 4 cut(s) 51, 1064, 1255, 1742
MaeI CTAG 2 cut(s) 909, 1097
MaeII ACGT 6 cut(s) 9, 316, 325, 380, 1413, 1422
MaeIII GTNAC 6 cut(s) 536, 572, 749, 811, 1172, 1597
MboII GAAGA 4 cut(s) 88, 870, 997, 1715
MflI RGATCY 2 cut(s) 260, 1111
MhlI GDGCHC 1 cut(s) 279
MluCI AATT 9 cut(s) 167, 593, 988, 1069, 1311, 1386, 1503, 1657, 1745
MlyI GAGTC 2 cut(s) 896, 1123
MmeI TCCRAC 2 cut(s) 207, 1280
Mph1103I ATGCAT 2 cut(s) 1198, 1270
MroI TCCGGA 1 cut(s) 115
MroXI GAANNNNTTC 4 cut(s) 886, 1092, 1441, 1560
MseI TTAA 3 cut(s) 207, 222, 783
MslI CAYNNNNRTG 4 cut(s) 426, 459, 801, 1148
MspA1I CMGCKG 2 cut(s) 1158, 1593
MspI CCGG 1 cut(s) 116
MspR9I CCNGG 3 cut(s) 132, 557, 1238
MvaI CCWGG 3 cut(s) 132, 557, 1238
MvnI CGCG 2 cut(s) 1158, 1498
MwoI GCNNNNNNNGC 4 cut(s) 36, 1009, 1739, 1748
NcoI CCATGG 1 cut(s) 1508
NlaIV GGNNCC 2 cut(s) 476, 1184
NmuCI GTSAC 4 cut(s) 536, 572, 749, 811
NruI TCGCGA 1 cut(s) 1498
NsiI ATGCAT 2 cut(s) 1198, 1270
NspI RCATGY 3 cut(s) 800, 1363, 1757
OliI CACNNNNGTG 1 cut(s) 1148
PagI TCATGA 2 cut(s) 421, 1462
PaqCI CACCTGC 1 cut(s) 791
PcsI WCGNNNNNNNCGW 3 cut(s) 322, 955, 1419
PdmI GAANNNNTTC 4 cut(s) 886, 1092, 1441, 1560
PfeI GAWTC 7 cut(s) 523, 722, 887, 1277, 1556, 1604, 1691
PflFI GACNNNGTC 1 cut(s) 8
PfoI TCCNGGA 1 cut(s) 555
PkrI GCNGC 4 cut(s) 41, 340, 1592, 1595
Ple19I CGATCG 1 cut(s) 16
PleI GAGTC 2 cut(s) 896, 1123
PmaCI CACGTG 1 cut(s) 1414
PmlI CACGTG 1 cut(s) 1414
PpsI GAGTC 2 cut(s) 896, 1123
Ppu21I YACGTR 3 cut(s) 317, 326, 1414
Psp6I CCWGG 3 cut(s) 130, 555, 1236
PspCI CACGTG 1 cut(s) 1414
PspGI CCWGG 3 cut(s) 130, 555, 1236
PspN4I GGNNCC 2 cut(s) 476, 1184
PspPI GGNCC 2 cut(s) 194, 1182
PstI CTGCAG 1 cut(s) 1478
PsuI RGATCY 2 cut(s) 260, 1111
PsyI GACNNNGTC 1 cut(s) 8
PvuI CGATCG 1 cut(s) 16
PvuII CAGCTG 1 cut(s) 1593
RruI TCGCGA 1 cut(s) 1498
RseI CAYNNNNRTG 4 cut(s) 426, 459, 801, 1148
SacII CCGCGG 1 cut(s) 1159
SaqAI TTAA 3 cut(s) 207, 222, 783
SatI GCNGC 4 cut(s) 40, 339, 1591, 1594
Sau96I GGNCC 2 cut(s) 194, 1182
SchI GAGTC 2 cut(s) 896, 1123
ScrFI CCNGG 3 cut(s) 132, 557, 1238
SduI GDGCHC 1 cut(s) 279
SfaNI GCATC 4 cut(s) 51, 1064, 1255, 1742
SfcI CTRYAG 2 cut(s) 1354, 1474
Sfr303I CCGCGG 1 cut(s) 1159
SgrBI CCGCGG 1 cut(s) 1159
SinI GGWCC 1 cut(s) 194
SmiMI CAYNNNNRTG 4 cut(s) 426, 459, 801, 1148
SnaBI TACGTA 1 cut(s) 326
Sse9I AATT 9 cut(s) 167, 593, 988, 1069, 1311, 1386, 1503, 1657, 1745
SsiI CCGC 6 cut(s) 39, 53, 60, 100, 1156, 1158
SspMI CTAG 2 cut(s) 909, 1097
StyD4I CCNGG 3 cut(s) 130, 555, 1236
StyI CCWWGG 2 cut(s) 386, 1508
TaaI ACNGT 3 cut(s) 272, 1178, 1379
TaiI ACGT 6 cut(s) 12, 319, 328, 383, 1416, 1425
TaqI TCGA 5 cut(s) 12, 24, 943, 1644, 1689
TasI AATT 9 cut(s) 167, 593, 988, 1069, 1311, 1386, 1503, 1657, 1745
TauI GCSGC 1 cut(s) 42
TfiI GAWTC 7 cut(s) 523, 722, 887, 1277, 1556, 1604, 1691
Tru1I TTAA 3 cut(s) 207, 222, 783
Tru9I TTAA 3 cut(s) 207, 222, 783
TscAI CASTG 3 cut(s) 820, 934, 999
TseFI GTSAC 4 cut(s) 536, 572, 749, 811
TseI GCWGC 3 cut(s) 338, 1590, 1593
Tsp45I GTSAC 4 cut(s) 536, 572, 749, 811
TspDTI ATGAA 9 cut(s) 411, 422, 536, 645, 747, 1039, 1551, 1596, 1716
TspGWI ACGGA 2 cut(s) 524, 844
TspRI CASTG 3 cut(s) 820, 934, 999
Tth111I GACNNNGTC 1 cut(s) 8
VpaK11BI GGWCC 1 cut(s) 194
XapI RAATTY 4 cut(s) 593, 988, 1311, 1657
XceI RCATGY 3 cut(s) 800, 1363, 1757
XmiI GTMKAC 1 cut(s) 1353
XmnI GAANNNNTTC 4 cut(s) 886, 1092, 1441, 1560
XspI CTAG 2 cut(s) 909, 1097
ZraI GACGTC 1 cut(s) 10
Zsp2I ATGCAT 2 cut(s) 1198, 1270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.