Rh1CG402000

DNA-directed primase polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0001804
Physical Location & Seq
Forward (+)
66789330 .. 66793397
4068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG402000.1

Sequence Viewer

Length: 1554 bp
ATGGACGACGTTGATCGGTTGTTCGAGTGCTTCAAGTGCGGCATCTCACCACCACCATCTGCTTTGAGAGAGAGAAAGAGAATCAAGAGGCAATCGATTCAGAGGAGTAAAGCCCAGGAAGTCGATTCTCCGCGGTCGCCGGAGCAGATAAAGAAAATCGCATCAGATTTACAGCTTTCTGCAGAAAAACTTGTTCAGGGTGGTTCTACAACAGTCGAAGCAAAAAGCCTTAATCGTAGGAAGCAGTTATCTCCAGTTGTATTTTACGGGTCTCCACATGGTGTACCTCCAAAGAAACCATCAAGGTTGTTGCTGCGAGTGTTACGTGAGATACGTGTTGATCTCACTGCACAGAAACGATTGAGCTCAAGTCTGTTTAGGAAGGAAGTATGGGCTACATTTCCAAGGCAGGAGGAAGCAATGCAGTTTGCCAAACGGCACGAGAATGTTCACGTTTTTAGTTATCAAGATCACTTTAGTGGACAAAGAAGGTTCCTTGTTTCAAGTTATGATGAGTTTTGGAGAAGGTACAAAATTATGGAGTCCAAGTTTCGTCACCATTACGAAGTCATTCAGGAGGGTTTACCATGCCACCTTTATTTTGACCTGGAGTTCAATAAGAAAGACAATGGAAACAGAAATGGAGATGAAATGGTTGATCTTTTAATATCAGTTGTTTTTGAAGCCTTGCTTGAAAAGTATTCTATTCAGGGGAACATGGAATGGATATTGGAACTTGATTCCTCTACTGAAGCAAAGTTCTCTCGACATTTAATTATTCGCATTCAGAAGACTGCTTTTAAGGACAACTCACATGTAGGTGCATTTGTCACCGAGATATGCTCACGGATATCAAGTGCAAAAGGGAGGGATGGAAGATTTGAGAATTTGTTTATTAGAAAAGATTCAAGCTCTGCAGACTTTAGTCACCTATTTGTGGACACTGCTGTGTATACCAGGAATCGTTGTTTTCGTTTAGCTCTATCATCAAAGGCAGGGAAGAATTCAGTGCTTCTACCTACTGGGCGCTTCAAAGCTAAGGACATGATGCAGTCTGAAGAGGAGATGTTCATGGCGTCTTTGATCTGCAATTTGGAAGTTGATTGTGAGAAGCTTCTAGTCTGTAAATCTGATCTTGATTGTATAAAGACCCTGCATTTTGATACAGAGGTCAACCCTAGTTTTGGAAAATCTTACAGTTGGCCCCAAGAATTTGCATTGAATGGTTGCACCAGTGATGCTTCAGCAACATACTTCCTGGGCAAATCTCCATTTCCAGCTTTGGATGCATTTGTAGAATTTGTTGCTACAATTGGAAATGTCTCAGGTAAAATTCGCAGCTGGTATTGGTTCTCAGAGTTTGGACTCATGGTCTACAGCATGTCAAGAAACAGATACTGTGAGCGAATTGGCAGACAGCACAAAAGCAATCATGTGATATACATTGCTGACCTCAGAAAGGGTGTTTATTATCAGAAATGTCATGATCCCGACTGCAGAGGTGAGGAAAAGTTAATATCGTGTACGTTTATCTTCTTTTGCTCTCTTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Herpes_UL52

Protein Analysis

517

Amino Acids

59.8

Weight (kDa)

9.03

Isoelectric Point (pI)

47.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Herpes_UL52 PF03121 450 - 502 3e-11 Herpesviridae UL52/UL70 DNA primase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 953, 1374
AccII CGCG 1 cut(s) 133
AciI CCGC 3 cut(s) 39, 131, 133
AclWI GGATC 1 cut(s) 1481
AcsI RAATTY 5 cut(s) 886, 1003, 1211, 1298, 1332
AcuI CTGAAG 3 cut(s) 771, 1077, 1227
AcyI GRCGYC 1 cut(s) 1076
AdeI CACNNNGTG 1 cut(s) 281
AfaI GTAC 3 cut(s) 285, 530, 1525
AfiI CCNNNNNNNGG 3 cut(s) 937, 1184, 1459
AflIII ACRYGT 2 cut(s) 334, 814
AgsI TTSAA 9 cut(s) 34, 504, 616, 683, 695, 909, 1033, 1222, 1550
AhdI GACNNNNNGTC 1 cut(s) 1370
AjnI CCWGG 4 cut(s) 114, 606, 956, 1257
AleI CACNNNNGTG 2 cut(s) 477, 947
AluBI AGCT 8 cut(s) 175, 366, 912, 980, 1037, 1114, 1280, 1341
AluI AGCT 8 cut(s) 175, 366, 912, 980, 1037, 1114, 1280, 1341
Alw21I GWGCWC 1 cut(s) 368
Alw26I GTCTC 2 cut(s) 276, 1327
AlwI GGATC 1 cut(s) 1481
AlwNI CAGNNNCTG 1 cut(s) 1398
AoxI GGCC 1 cut(s) 1202
ApeKI GCWGC 2 cut(s) 313, 1338
ApoI RAATTY 5 cut(s) 886, 1003, 1211, 1298, 1332
Asp700I GAANNNNTTC 2 cut(s) 570, 904
AspLEI GCGC 1 cut(s) 1029
AspS9I GGNCC 1 cut(s) 1203
AsuHPI GGTGA 5 cut(s) 39, 548, 823, 920, 1514
BaeI ACNNNNGTAYC 2 cut(s) 1155, 1188
BanII GRGCYC 1 cut(s) 368
BauI CACGAG 1 cut(s) 440
BbsI GAAGAC 1 cut(s) 797
Bbv12I GWGCWC 1 cut(s) 368
BbvI GCAGC 2 cut(s) 300, 1350
BccI CCATC 3 cut(s) 64, 307, 866
BceAI ACGGC 1 cut(s) 452
BciT130I CCWGG 4 cut(s) 116, 608, 958, 1259
BcoDI GTCTC 2 cut(s) 276, 1327
BfaI CTAG 2 cut(s) 1118, 1179
BfmI CTRYAG 4 cut(s) 180, 915, 1375, 1495
BfoI RGCGCY 1 cut(s) 1030
BisI GCNGC 3 cut(s) 40, 314, 1339
BlsI GCNGC 3 cut(s) 41, 315, 1340
Bme1390I CCNGG 4 cut(s) 116, 608, 958, 1259
BmeRI GACNNNNNGTC 1 cut(s) 1370
BmgT120I GGNCC 1 cut(s) 1203
BmiI GGNNCC 2 cut(s) 494, 1205
BmrFI CCNGG 4 cut(s) 116, 608, 958, 1259
BmrI ACTGGG 1 cut(s) 1032
BmsI GCATC 5 cut(s) 51, 170, 1038, 1228, 1276
BmuI ACTGGG 1 cut(s) 1032
BoxI GACNNNNGTC 1 cut(s) 924
BpiI GAAGAC 1 cut(s) 797
BplI GAGNNNNNCTC 2 cut(s) 827, 859
BpmI CTGGAG 2 cut(s) 237, 629
Bpu10I CCTNAGC 1 cut(s) 1038
BpuEI CTTGAG 1 cut(s) 352
Bsa29I ATCGAT 1 cut(s) 95
BsaAI YACGTR 2 cut(s) 326, 335
BsaHI GRCGYC 1 cut(s) 1076
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 4 cut(s) 114, 131, 404, 1258
BsaXI ACNNNNNCTCC 2 cut(s) 533, 563
Bsc4I CCNNNNNNNGG 3 cut(s) 937, 1184, 1459
Bse1I ACTGG 3 cut(s) 254, 1027, 1233
Bse3DI GCAATG 2 cut(s) 426, 1443
BseBI CCWGG 4 cut(s) 116, 608, 958, 1259
BseCI ATCGAT 1 cut(s) 95
BseDI CCNNGG 4 cut(s) 114, 131, 404, 1258
BseGI GGATG 2 cut(s) 877, 1291
BseLI CCNNNNNNNGG 3 cut(s) 937, 1184, 1459
BseMI GCAATG 2 cut(s) 426, 1443
BseMII CTCAG 3 cut(s) 1338, 1368, 1468
BseNI ACTGG 3 cut(s) 254, 1027, 1233
BseRI GAGGAG 2 cut(s) 118, 1076
BseXI GCAGC 2 cut(s) 300, 1350
BsgI GTGCAG 1 cut(s) 333
Bsh1236I CGCG 1 cut(s) 133
Bsh1285I CGRYCG 1 cut(s) 137
BshFI GGCC 1 cut(s) 1204
BshVI ATCGAT 1 cut(s) 95
BsiEI CGRYCG 1 cut(s) 137
BsiHKAI GWGCWC 1 cut(s) 368
BsiSI CCGG 1 cut(s) 140
BslI CCNNNNNNNGG 3 cut(s) 937, 1184, 1459
BsmAI GTCTC 2 cut(s) 276, 1327
BsmI GAATGC 1 cut(s) 783
BsnI GGCC 1 cut(s) 1204
Bso31I GGTCTC 1 cut(s) 276
Bsp1286I GDGCHC 1 cut(s) 368
Bsp143I GATC 7 cut(s) 13, 340, 469, 658, 1083, 1132, 1486
BspACI CCGC 3 cut(s) 39, 131, 133
BspANI GGCC 1 cut(s) 1204
BspCNI CTCAG 3 cut(s) 1337, 1367, 1467
BspDI ATCGAT 1 cut(s) 95
BspFNI CGCG 1 cut(s) 133
BspHI TCATGA 1 cut(s) 1483
BspLI GGNNCC 2 cut(s) 494, 1205
BspMAI CTGCAG 3 cut(s) 184, 919, 1499
BspPI GGATC 1 cut(s) 1481
BspTNI GGTCTC 1 cut(s) 276
BsrDI GCAATG 2 cut(s) 426, 1443
BsrI ACTGG 3 cut(s) 254, 1027, 1233
BssECI CCNNGG 4 cut(s) 114, 131, 404, 1258
BssMI GATC 7 cut(s) 13, 340, 469, 658, 1083, 1132, 1486
BssNAI GTATAC 1 cut(s) 954
BssNI GRCGYC 1 cut(s) 1076
BssSI CACGAG 1 cut(s) 440
BssT1I CCWWGG 1 cut(s) 404
Bst1107I GTATAC 1 cut(s) 954
Bst2BI CACGAG 1 cut(s) 440
Bst2UI CCWGG 4 cut(s) 116, 608, 958, 1259
Bst4CI ACNGT 3 cut(s) 214, 1199, 1400
Bst6I CTCTTC 2 cut(s) 1053, 1551
BstACI GRCGYC 1 cut(s) 1076
BstBAI YACGTR 2 cut(s) 326, 335
BstDEI CTNAG 4 cut(s) 1038, 1324, 1354, 1454
BstDSI CCRYGG 1 cut(s) 131
BstENI CCTNNNNNAGG 1 cut(s) 1457
BstF5I GGATG 2 cut(s) 877, 1291
BstFNI CGCG 1 cut(s) 133
BstH2I RGCGCY 1 cut(s) 1030
BstHHI GCGC 1 cut(s) 1029
BstKTI GATC 7 cut(s) 16, 343, 472, 661, 1086, 1135, 1489
BstMAI GTCTC 2 cut(s) 276, 1327
BstMBI GATC 7 cut(s) 13, 340, 469, 658, 1083, 1132, 1486
BstMCI CGRYCG 1 cut(s) 137
BstMWI GCNNNNNNNGC 2 cut(s) 36, 1286
BstNI CCWGG 4 cut(s) 116, 608, 958, 1259
BstNSI RCATGY 2 cut(s) 818, 1384
BstPAI GACNNNNGTC 1 cut(s) 924
BstSCI CCNGG 4 cut(s) 114, 606, 956, 1257
BstSFI CTRYAG 4 cut(s) 180, 915, 1375, 1495
BstUI CGCG 1 cut(s) 133
BstV1I GCAGC 2 cut(s) 300, 1350
BstV2I GAAGAC 1 cut(s) 797
BstZ17I GTATAC 1 cut(s) 954
Bsu15I ATCGAT 1 cut(s) 95
BsuRI GGCC 1 cut(s) 1204
BsuTUI ATCGAT 1 cut(s) 95
BtgI CCRYGG 1 cut(s) 131
BtsCI GGATG 2 cut(s) 877, 1291
BtsI GCAGTG 2 cut(s) 345, 942
BtsIMutI CAGTG 4 cut(s) 345, 942, 1014, 1240
CaiI CAGNNNCTG 1 cut(s) 1398
CciI TCATGA 1 cut(s) 1483
CfoI GCGC 1 cut(s) 1029
Cfr13I GGNCC 1 cut(s) 1203
Cfr42I CCGCGG 1 cut(s) 134
ClaI ATCGAT 1 cut(s) 95
CseI GACGC 1 cut(s) 1065
Csp6I GTAC 3 cut(s) 284, 529, 1524
CviQI GTAC 3 cut(s) 284, 529, 1524
DdeI CTNAG 4 cut(s) 1038, 1324, 1354, 1454
DpnI GATC 7 cut(s) 15, 342, 471, 660, 1085, 1134, 1488
DpnII GATC 7 cut(s) 13, 340, 469, 658, 1083, 1132, 1486
DraIII CACNNNGTG 1 cut(s) 281
DriI GACNNNNNGTC 1 cut(s) 1370
Eam1104I CTCTTC 2 cut(s) 1053, 1551
Eam1105I GACNNNNNGTC 1 cut(s) 1370
EarI CTCTTC 2 cut(s) 1053, 1551
Ecl136II GAGCTC 1 cut(s) 366
Eco130I CCWWGG 1 cut(s) 404
Eco24I GRGCYC 1 cut(s) 368
Eco31I GGTCTC 1 cut(s) 276
Eco32I GATATC 1 cut(s) 852
Eco53kI GAGCTC 1 cut(s) 366
Eco57I CTGAAG 3 cut(s) 771, 1077, 1227
EcoICRI GAGCTC 1 cut(s) 366
EcoNI CCTNNNNNAGG 1 cut(s) 1457
EcoRI GAATTC 1 cut(s) 1003
EcoRII CCWGG 4 cut(s) 114, 606, 956, 1257
EcoRV GATATC 1 cut(s) 852
EcoT14I CCWWGG 1 cut(s) 404
EcoT22I ATGCAT 1 cut(s) 1291
EcoT38I GRGCYC 1 cut(s) 368
ErhI CCWWGG 1 cut(s) 404
FalI AAGNNNNNCTT 2 cut(s) 675, 707
FblI GTMKAC 2 cut(s) 953, 1374
Fnu4HI GCNGC 3 cut(s) 40, 314, 1339
FokI GGATG 2 cut(s) 884, 1298
FriOI GRGCYC 1 cut(s) 368
Fsp4HI GCNGC 3 cut(s) 40, 314, 1339
FspBI CTAG 2 cut(s) 1118, 1179
GlaI GCGC 1 cut(s) 1028
GluI GCNGC 3 cut(s) 40, 314, 1339
GsuI CTGGAG 2 cut(s) 237, 629
HaeII RGCGCY 1 cut(s) 1030
HaeIII GGCC 1 cut(s) 1204
HapII CCGG 1 cut(s) 140
HgaI GACGC 1 cut(s) 1065
HhaI GCGC 1 cut(s) 1029
Hin1I GRCGYC 1 cut(s) 1076
Hin6I GCGC 1 cut(s) 1027
HinP1I GCGC 1 cut(s) 1027
HincII GTYRAC 1 cut(s) 1174
HindII GTYRAC 1 cut(s) 1174
HindIII AAGCTT 1 cut(s) 1112
HinfI GANTC 8 cut(s) 81, 97, 125, 542, 740, 905, 961, 1365
HpaII CCGG 1 cut(s) 140
HphI GGTGA 5 cut(s) 39, 548, 823, 920, 1514
Hpy166II GTNNAC 9 cut(s) 284, 451, 482, 584, 940, 954, 1174, 1375, 1524
Hpy188I TCNGA 8 cut(s) 102, 166, 789, 1057, 1132, 1357, 1457, 1476
Hpy188III TCNNGA 8 cut(s) 85, 467, 575, 765, 1136, 1386, 1484, 1490
Hpy8I GTNNAC 9 cut(s) 284, 451, 482, 584, 940, 954, 1174, 1375, 1524
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 3 cut(s) 376, 483, 519
HpyCH4III ACNGT 3 cut(s) 214, 1199, 1400
HpyCH4IV ACGT 5 cut(s) 9, 325, 334, 453, 1526
HpyF10VI GCNNNNNNNGC 2 cut(s) 36, 1286
HpyF3I CTNAG 4 cut(s) 1038, 1324, 1354, 1454
HpySE526I ACGT 5 cut(s) 9, 325, 334, 453, 1526
Hsp92I GRCGYC 1 cut(s) 1076
HspAI GCGC 1 cut(s) 1027
KspI CCGCGG 1 cut(s) 134
Kzo9I GATC 7 cut(s) 13, 340, 469, 658, 1083, 1132, 1486
LmnI GCTCC 1 cut(s) 142
Lsp1109I GCAGC 2 cut(s) 300, 1350
LweI GCATC 5 cut(s) 51, 170, 1038, 1228, 1276
MaeI CTAG 2 cut(s) 1118, 1179
MaeII ACGT 5 cut(s) 9, 325, 334, 453, 1526
MaeIII GTNAC 4 cut(s) 321, 554, 829, 926
MalI GATC 7 cut(s) 15, 342, 471, 660, 1085, 1134, 1488
MboI GATC 7 cut(s) 13, 340, 469, 658, 1083, 1132, 1486
MboII GAAGA 6 cut(s) 802, 888, 1012, 1070, 1525, 1538
MfeI CAATTG 1 cut(s) 1311
MhlI GDGCHC 1 cut(s) 368
MlyI GAGTC 2 cut(s) 551, 1359
Mph1103I ATGCAT 1 cut(s) 1291
MroXI GAANNNNTTC 2 cut(s) 570, 904
MseI TTAA 5 cut(s) 231, 665, 773, 801, 1514
MslI CAYNNNNRTG 4 cut(s) 444, 477, 819, 947
MspA1I CMGCKG 2 cut(s) 133, 1341
MspI CCGG 1 cut(s) 140
MspR9I CCNGG 4 cut(s) 116, 608, 958, 1259
MunI CAATTG 1 cut(s) 1311
Mva1269I GAATGC 1 cut(s) 783
MvaI CCWGG 4 cut(s) 116, 608, 958, 1259
MvnI CGCG 1 cut(s) 133
MwoI GCNNNNNNNGC 2 cut(s) 36, 1286
NdeII GATC 7 cut(s) 13, 340, 469, 658, 1083, 1132, 1486
NlaIV GGNNCC 2 cut(s) 494, 1205
NmuCI GTSAC 3 cut(s) 554, 829, 926
NsiI ATGCAT 1 cut(s) 1291
NspI RCATGY 2 cut(s) 818, 1384
OliI CACNNNNGTG 2 cut(s) 477, 947
PagI TCATGA 1 cut(s) 1483
PciI ACATGT 1 cut(s) 814
PcsI WCGNNNNNNNCGW 2 cut(s) 331, 970
PctI GAATGC 1 cut(s) 783
PdmI GAANNNNTTC 2 cut(s) 570, 904
PfeI GAWTC 6 cut(s) 81, 97, 125, 740, 905, 961
PkrI GCNGC 3 cut(s) 41, 315, 1340
PleI GAGTC 2 cut(s) 550, 1359
PpsI GAGTC 2 cut(s) 550, 1359
Ppu21I YACGTR 2 cut(s) 326, 335
PscI ACATGT 1 cut(s) 814
PshAI GACNNNNGTC 1 cut(s) 924
Psp124BI GAGCTC 1 cut(s) 368
Psp6I CCWGG 4 cut(s) 114, 606, 956, 1257
PspGI CCWGG 4 cut(s) 114, 606, 956, 1257
PspN4I GGNNCC 2 cut(s) 494, 1205
PspPI GGNCC 1 cut(s) 1203
PstI CTGCAG 3 cut(s) 184, 919, 1499
PstNI CAGNNNCTG 1 cut(s) 1398
PvuII CAGCTG 1 cut(s) 1341
RsaI GTAC 3 cut(s) 285, 530, 1525
RsaNI GTAC 3 cut(s) 284, 529, 1524
RseI CAYNNNNRTG 4 cut(s) 444, 477, 819, 947
SacI GAGCTC 1 cut(s) 368
SacII CCGCGG 1 cut(s) 134
SaqAI TTAA 5 cut(s) 231, 665, 773, 801, 1514
SatI GCNGC 3 cut(s) 40, 314, 1339
Sau3AI GATC 7 cut(s) 13, 340, 469, 658, 1083, 1132, 1486
Sau96I GGNCC 1 cut(s) 1203
SchI GAGTC 2 cut(s) 551, 1359
ScrFI CCNGG 4 cut(s) 116, 608, 958, 1259
SduI GDGCHC 1 cut(s) 368
SfaNI GCATC 5 cut(s) 51, 170, 1038, 1228, 1276
SfcI CTRYAG 4 cut(s) 180, 915, 1375, 1495
Sfr303I CCGCGG 1 cut(s) 134
SgrBI CCGCGG 1 cut(s) 134
SmiMI CAYNNNNRTG 4 cut(s) 444, 477, 819, 947
SmlI CTYRAG 1 cut(s) 367
SmoI CTYRAG 1 cut(s) 367
SsiI CCGC 3 cut(s) 39, 131, 133
SspMI CTAG 2 cut(s) 1118, 1179
SstI GAGCTC 1 cut(s) 368
StyD4I CCNGG 4 cut(s) 114, 606, 956, 1257
StyI CCWWGG 1 cut(s) 404
TaaI ACNGT 3 cut(s) 214, 1199, 1400
TaiI ACGT 5 cut(s) 12, 328, 337, 456, 1529
TaqI TCGA 5 cut(s) 24, 95, 123, 216, 766
TauI GCSGC 1 cut(s) 42
TfiI GAWTC 6 cut(s) 81, 97, 125, 740, 905, 961
Tru1I TTAA 5 cut(s) 231, 665, 773, 801, 1514
Tru9I TTAA 5 cut(s) 231, 665, 773, 801, 1514
TscAI CASTG 4 cut(s) 352, 949, 1014, 1240
TseFI GTSAC 3 cut(s) 554, 829, 926
TseI GCWGC 2 cut(s) 313, 1338
Tsp45I GTSAC 3 cut(s) 554, 829, 926
TspDTI ATGAA 2 cut(s) 663, 1060
TspGWI ACGGA 1 cut(s) 862
TspRI CASTG 4 cut(s) 352, 949, 1014, 1240
XagI CCTNNNNNAGG 1 cut(s) 1457
XapI RAATTY 5 cut(s) 886, 1003, 1211, 1298, 1332
XceI RCATGY 2 cut(s) 818, 1384
XmiI GTMKAC 2 cut(s) 953, 1374
XmnI GAANNNNTTC 2 cut(s) 570, 904
XspI CTAG 2 cut(s) 1118, 1179
Zsp2I ATGCAT 1 cut(s) 1291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.