Rorug01G0409400

DNA-directed primase polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001804
Physical Location & Seq
Reverse (-)
51515432 .. 51525666
10235 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0409400.1

Sequence Viewer

Length: 5049 bp
ATGTTTGTGTTGGAATTGTGGTTTCAGATGTATAGGATGGGTAGAAGGAAGCAAAACCGGCCTCACCGGTCGGGTGGGATTGTATTAGAGAGTCAAGCAACTGCTGGCAGGGGAGAATTGGATGAGGGGAGGCTTTTAAATGGACAAACGCAAAAGAATGACATAGATGAAGTTGATAGACCGTATTTTGTGGAAGTTGATCGGTCTAATTGGGGATCAGATGAGCACCTTGATATTGCTGAAGTGGTTTTAACAGATTTGACTTTCGGAGAAGGGTTTTGCAGTGATGTTGTTAGTGGGGGTTTGAATTGCGATAGTTATTCCTTAAGGTTCCGGTTATCTAATGTGAAGGAGTTCATTGGTCGTATTAAGCTTGGTCATTGGCCTTTGTTGTCCTCTGCTGATATATCTTTGGAATTCGTTAAAGCATGTTCAAGCGAGGATATGGACATGGAGAAGGACACGTGCTCAGTAATCTTGTCAGGGAGTTTTGATGGACCAGATGAAGGTATTTCTGGTCTGGTACACTTGGCAAGTTTGAAGTTTATGACACTGAGGCCAGCTCTGTCCATTGGGTTTGTGAATGATACGTCAACCATCAGGGTCAGGGTGGAGATACTTAAGAGTGCATTTAATGCTTGTGAGTCACTTCTGGACAATACTAGGCAAGTGTGGAAAAAGAGTATGATGAATGTCATGGCTTGGCTACACCCAGAAGTAGTGACTTCCGAGGCTAGGTATGGAGTCAGTAGATCAACAGACATGGAAATTGATTCGCATACTGACACTGGACATGCTAATTCAAATCATAGTAAACATGCAAGGTTTGATGTTGCTGGGTTCTATGAAGCCATTAAACCCTCAAAAGCAGATGCAATGCTTCAAGATGTCCTGCCTGATTTGCTTCCTGAATTGAAACCATATCAGCGCCGTGCAGCTTACTGGATGGTACAGAGGGAGAAAAGCGATGCACAAAGTCTGGCCCAAGAGGCCAAAAGTCAGTTCATGTCGCCTTTATGTTTGCCCTTGCAATTTCTCGACAGATGTTCAAAAATGTTCTACAACCCATTCAGTGGAAATGTTTCGCTGGACCAAGAGCATTTTTCAACGTACGTCTCTGGTGGTATTCTTGCTGATGAGATGGGCATGGGGAAAACTGTTGAACTTCTCGCTTGCATCTTTGCTCATCAGAAGTCAGCAGATGAAGACCCCATATTTGCTGATGCTGAAATTCAAGATACTGAGGATCTGAAAATAAAGCTTAAAAGATTAAAAAGGGAGCGAGTTGAATGTATATGTGGAGCTGTGAGTGAAAGTATCAGTTACAGAGGACTATGGGTACAGTGCGACATATGTGATGCATGGCAACATGCCGATTGTGTGGGCTATTCACCTAGAGGAAAGACTATAAAATCCAACGAGGCTTCTAACCAAAAAGAACATGATAAGAGTTCGGTGGTTAAGAATTATACTAGAAAGAAGAATACAGCCACAATAGATGTGAGAGATGAGGAATATATATGCCAGCTGTGCTCAGAACTGATACAGGCCACTAATTCTCCTGTTGCTACTGGTGCCACTCTTATAATCTGTCCAGCTTCTATATTGCCCCAGTGGTATTCTGAAATTATGCGCCATACGCGTTCAGGTTCTTTAAAGACTTGCGTCTATGAAGGTGTAAGAGAGGCATCTTTTTCAAACACATCTGTGATTGATATCAGTGAACTCATCAGTGCTGACATTGTGTTGACAACATATGATGTGCTTAAAGCGGACCTGTCACATGACTCTGATAGGCATGAAGGTGATCGACGCTTAATGAGATTCCAAAAGAGATACCCTGTTGTTCCAACTATTCTTACCAGAATATTCTGGTGGAGAATTTGTTTGGATGAGGCTCAGATGGTGGAGAGTAATGCTGGTGCTGCAACAGAAATGGCTATGCGACTGTATGCGAAGCATCATTGGTGCATAACGGGGACTCCTATACAACGTAAACTTGATGACTTATATGGACTTCTACGGTTTCTTAAAGCATGCCCTTTCAATGCTTCCAGGTGGTGGATTGAAGTTATACGAGATCCATATGAGAGGAGAGATGCAGGAGCTATGGAATTTACACATAAATTCTTTAAAGAAATCATGTGGCGATCTTCAAAAGTGCATGTTGCAGATGAGTTGCAGCTTCCACCTCAGGAAGAATGTCTCTCTTGGCTCACTTTATCTCCAGTTGAAGAGCATTTTTACCAGAGGCAACATGAAACTTGTGTGAGTTATGCCCGAGAAGTCATTGAACATATGAAAGATGATATTATGAAGAGAAAAGTCAGAGGATGCTCAGCAGCTAAATCATCAGATTCTTTCATCACACATGCGGAGGCAGGAAAGCTGCTGAACACACTTCTGAAGCTTCGCCAAGCCAGCTGTCACCCTCAAGTAGGAAGCTCAGGACTGCGTTCTCTCCAACAGTCTCCCATGACTATGGAGGAAATATTAATGGTTCTTGTAAGTAAAACCAAGATAGAAGGAGAGGAAGCTCTCAGAAGGTTAGTTGTTGCTCTTAATGGGCTCGCTGGGATAGCTGTAATTGAGCAAAATTTTACTCAAGCTGTATCACTGTATAAGGAATCGTTGACTTTAGCTGAAGAGCACACTGAGGATTTCCGCCTGGATCCTTTGTTGAATATTCACATTCATCATAATCTTGCCGAAATACTTCCATTAGCAACAAGCTGCTCCCCTTCAAAAGGACAACATATTCCTGGAAAAATGGGCCCCTGTGATGAACATGTAGCAAAGAGGCGAAAACTGGGTGGGGAAGATAACTCATCAGAGAATGACGTATCTAATGCTCAAGAATATAATAACTCCCATGCATCATGTAGTTCCTTCACTGACGTATCTTTGAGAACAGTCTGTGACAATCTTAAACAAAAGTACTTATCTGCATTCAGTTCAAAGTTATTCATGACTCAGCAAGAGTTTAAGAAGTCTTACACCCAGGTTTGTAGCACAATTAGTGAGATCAAAGATGTAAGTACAGTCTGGTGGCTGGAAGCCCTTCTTCATGCTGAAAAGAATAATGACATTTCTAGTCTGTTGATCAGAAAGATTGAAGAGGCGCTCATAGGAACTCTAAACAATTCAAAGTCATCTAGAATTCCATCTCGTTTACGAAGCATAAGCGCTCTAAAGTATCACATGCAGACCGGTTTGGACCAACTAGAAGAATCTAGGAAACTGTTACTTGATCGACTTTTAGAAATTGATCAGACAATGGAGAAGCCAAAAGAGGAGGATATCCAGCGTGTGAGATACTGTCGAAATTGCAAAGCTAATGATGGTGGTCCCTTATGTGTTCTTTGTGAAGTAGATGAATTATTTCGGGGATATGAAGCAAGGCTCTTTCGTCTTGAGAAAATATCCGGAGGGATTGCTACCTCTGCTGAAGAGGCTGTGGATTTGCAGAAGAAGAACTCTGAACTTAATCGTTTCTATCAGAATTTATCACAGCCAATTAAGGATTCAACGTCATCCAAATCTAATCAAGAATCGAAGAAGAGAGATGTTGGAAAAGTTGTGGTTTCAAAATCCCCATCTGAATTAGAGGTTGTTCTTGGAGTTATAAAAAGCCATTGCAAGGCCCATTTAGGAAAGGAAGGTATATCAGAAGCTACCAAGCACCTACACATTCTTGAGGGCATGCGCAAGGAGTATGGGCATGCAAGATCCTTGGCAATAGCTCAAGCTCAGATTCTGCAAGCTTATGATGAAATTAATATGGCAACTACAAGGTTGCGCCTGAGTGAAGATGAGAATGATAAATCTCTTGATGTATTAAGTGAAGATGAATTACCTTCAGCTAACGTTTTATACACCAGTGAAAAGTTCGCATCCTTGAGCTTGTTGTCTTGTATAAAAGGGAAGCTTCGTTATTTGAAGGGTTTGGTAGAAGCTAAACAAAAAATGCCATTTGGAAGTCCAAATCATTCCTCAGTAACTGGAGAGGAAGCCACCATATTGACTTCTACAGAACAGAGAAATGAATGTGTACCTACAGGTGATAAAGAAACGTGCCCAGTTTGTCAAGAACAGTTAACCATTCGAAAGATGGTTTTCCCTTGTGGACATCTTACTTGCTGTAAATGTTTATTTGCAATTACTGAGAGACTACTAAATGGGAAAAAGGTTCAAGATAAGTGGGTAAAATGCCCAACATGTCGACAGCACACTGATGTTGCAAATATTGCTTTTGCTGATGATGGACAAAGTGAATCTTGTGGTTCTGCTGGGCTTCATGCAATCCAAAGTCGTGAAGAGTGTGAAGCATCTATTATAGTTCAAGGTTCATATGGAACAAAGATTGAAGCAGTTACAAGAAGAATTCTGTGGATAAAGTCTACCGATCCAGAGGCTAAAGTTCTTGTTTTCTCAAGCTGGAATGATGTCCTTGACGTATTGGAGCATGCCTTCATTGCTAATGGCATTACCTTTATAAGGATGAAAGGAGGAAGGAAATCACAAGTTGCCATTAGTGAATTTAAAGGAGAAAAGAGAAGCTTAACAGGAAATCACAAGTTGCATGGCCGAAACCCAGAAGGAAGATCTATTCAAGTGTTGCTGCTCTTAATTCAACATGGGGCGAATGGCCTCAATCTGTTGGAAGCGAAGCATGTTATTCTTATTGAGCCTCTTCTCAATCCAGCAGCAGAAGCACAAGCCATCAGCAGGGTACACCGTATCGGACAGAAAAATAAGACCCTCGCTCATCGTTTCATAGTTAAAGGCACAGTTGAAGAGAGCATATACAAGCTCAACAAAAGCAGGAACAGCACTGCCTTCATTAGTGGTAACACAAAAAACCAAGATCAGCCCTTATTGACACTGAAAGACATCGAGGCCTTATTTTCAACAGTGCCATCATTACCTGTACCAGAGACTGATGTAAGGCCAACTGAAAGTGAAACTCTAAGACATTTGCCACCTTCTGTGGCTGCCGCCATTGCAGCTGAGAAGCGGCTCAAGGAGCAGCATTCATGTTCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Herpes_UL52

Protein Analysis

1682

Amino Acids

188.88

Weight (kDa)

6.47

Isoelectric Point (pI)

46.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNF2-rel_dom PF00176 308 - 812 1.9e-62 SNF2-related domain
SHPRH_helical-1st PF21325 828 - 907 2.4e-24 E3 ubiquitin-protein ligase SHPRH, first helical domain
SHPRH_helical-2nd PF21324 1014 - 1138 1.1e-06 E3 ubiquitin-protein ligase SHPRH, second helical domain
Helicase_C PF00271 1456 - 1582 7.2e-07 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1586, 3626, 4496
Acc16I TGCGCA 1 cut(s) 3707
AccB1I GGYRCC 1 cut(s) 1574
AccB7I CCANNNNNTGG 2 cut(s) 1073, 2061
AccI GTMKAC 2 cut(s) 4222, 4400
AccII CGCG 1 cut(s) 1642
AccIII TCCGGA 1 cut(s) 3425
AciI CCGC 5 cut(s) 1772, 2375, 2665, 4998, 5017
AclI AACGTT 1 cut(s) 3867
AclWI GGATC 7 cut(s) 223, 1254, 2075, 2666, 2679, 3723, 4400
AcoI YGGCCR 1 cut(s) 4585
AcuI CTGAAG 5 cut(s) 261, 2426, 2664, 3468, 3843
AcvI CACGTG 1 cut(s) 465
AfaI GTAC 9 cut(s) 525, 951, 1112, 1341, 2939, 3040, 4053, 4734, 4932
AfeI AGCGCT 1 cut(s) 3187
AfiI CCNNNNNNNGG 9 cut(s) 506, 735, 1073, 2061, 2438, 2747, 3640, 4497, 4728
AflII CTTAAG 2 cut(s) 325, 620
AflIII ACRYGT 4 cut(s) 462, 1640, 2788, 4217
AgeI ACCGGT 2 cut(s) 66, 3209
AjnI CCWGG 4 cut(s) 2054, 2667, 2761, 3000
AjuI GAANNNNNNNTTGG 4 cut(s) 986, 1018, 3508, 3540
AleI CACNNNNGTG 1 cut(s) 1706
Alw21I GWGCWC 4 cut(s) 228, 470, 1535, 2652
Alw26I GTCTC 5 cut(s) 1120, 2210, 2475, 4162, 4931
AlwI GGATC 7 cut(s) 223, 1254, 2075, 2666, 2679, 3723, 4400
AlwNI CAGNNNCTG 3 cut(s) 3757, 4001, 4940
Ama87I CYCGRG 1 cut(s) 2280
Aor13HI TCCGGA 1 cut(s) 3425
Aor51HI AGCGCT 1 cut(s) 3187
ApaI GGGCCC 1 cut(s) 2777
ArsI GACNNNNNNTTYG 2 cut(s) 1199, 1231
AseI ATTAAT 2 cut(s) 2495, 3777
AsiGI ACCGGT 2 cut(s) 66, 3209
Asp700I GAANNNNTTC 5 cut(s) 353, 1081, 2715, 3060, 3381
AspLEI GCGC 6 cut(s) 930, 1635, 3124, 3188, 3708, 3801
AspS9I GGNCC 9 cut(s) 497, 982, 1090, 1774, 2773, 2774, 3217, 3347, 3643
AsuHPI GGTGA 5 cut(s) 56, 1383, 1817, 2420, 4073
AsuII TTCGAA 1 cut(s) 4105
AvaI CYCGRG 1 cut(s) 2280
AvaII GGWCC 5 cut(s) 497, 1090, 1774, 3217, 3347
AxyI CCTNAGG 1 cut(s) 2193
BaeGI GKGCMC 2 cut(s) 2777, 4079
BaeI ACNNNNGTAYC 2 cut(s) 2883, 2916
BamHI GGATCC 1 cut(s) 2671
BanI GGYRCC 1 cut(s) 1574
BanII GRGCYC 2 cut(s) 2571, 2777
BarI GAAGNNNNNNTAC 2 cut(s) 3837, 3869
BbrPI CACGTG 1 cut(s) 465
BbsI GAAGAC 1 cut(s) 1212
Bbv12I GWGCWC 4 cut(s) 228, 470, 1535, 2652
BceAI ACGGC 1 cut(s) 915
BciT130I CCWGG 4 cut(s) 2056, 2669, 2763, 3002
BclI TGATCA 2 cut(s) 3102, 3268
BcoDI GTCTC 5 cut(s) 1120, 2210, 2475, 4162, 4931
BfaI CTAG 8 cut(s) 663, 735, 1395, 1473, 3093, 3156, 3224, 3234
BfmI CTRYAG 2 cut(s) 4029, 4056
BfoI RGCGCY 3 cut(s) 931, 3125, 3189
BfrI CTTAAG 2 cut(s) 325, 620
BglI GCCNNNNNGGC 1 cut(s) 989
BglII AGATCT 1 cut(s) 4604
BlpI GCTNAGC 1 cut(s) 2338
BmcAI AGTACT 1 cut(s) 2939
Bme1390I CCNGG 4 cut(s) 2056, 2669, 2763, 3002
Bme18I GGWCC 5 cut(s) 497, 1090, 1774, 3217, 3347
BmeT110I CYCGRG 1 cut(s) 2280
BmgT120I GGNCC 9 cut(s) 497, 982, 1090, 1774, 2773, 2774, 3217, 3347, 3643
BmiI GGNNCC 6 cut(s) 332, 1576, 2673, 2775, 2776, 3349
BmrFI CCNGG 4 cut(s) 2056, 2669, 2763, 3002
BmrI ACTGGG 3 cut(s) 1606, 2819, 4073
BmuI ACTGGG 3 cut(s) 1606, 2819, 4073
BoxI GACNNNNGTC 1 cut(s) 1664
BpiI GAAGAC 1 cut(s) 1212
BplI GAGNNNNNCTC 2 cut(s) 547, 579
BpmI CTGGAG 2 cut(s) 2211, 4023
Bpu10I CCTNAGC 1 cut(s) 2446
Bpu1102I GCTNAGC 1 cut(s) 2338
Bpu14I TTCGAA 1 cut(s) 4105
BpuEI CTTGAG 9 cut(s) 2418, 2589, 2838, 3434, 3716, 3729, 3919, 4417, 5006
BsaAI YACGTR 1 cut(s) 465
BsaBI GATNNNNATC 1 cut(s) 1715
BsaJI CCNNGG 3 cut(s) 729, 3000, 3732
BsaWI WCCGGW 4 cut(s) 66, 333, 3209, 3425
BsaXI ACNNNNNCTCC 8 cut(s) 1543, 1573, 1870, 1900, 1966, 1996, 2209, 2239
Bsc4I CCNNNNNNNGG 9 cut(s) 506, 735, 1073, 2061, 2438, 2747, 3640, 4497, 4728
Bse118I RCCGGY 3 cut(s) 57, 66, 3209
Bse1I ACTGG 9 cut(s) 793, 947, 1576, 1612, 2228, 2814, 3879, 4006, 4079
Bse21I CCTNAGG 1 cut(s) 2193
Bse3DI GCAATG 4 cut(s) 882, 3634, 4473, 5001
Bse8I GATNNNNATC 1 cut(s) 1715
BseAI TCCGGA 1 cut(s) 3425
BseBI CCWGG 4 cut(s) 2056, 2669, 2763, 3002
BseDI CCNNGG 3 cut(s) 729, 3000, 3732
BseGI GGATG 8 cut(s) 42, 127, 951, 1897, 2339, 3533, 3893, 4506
BseJI GATNNNNATC 1 cut(s) 1715
BseLI CCNNNNNNNGG 9 cut(s) 506, 735, 1073, 2061, 2438, 2747, 3640, 4497, 4728
BseMI GCAATG 4 cut(s) 882, 3634, 4473, 5001
BseNI ACTGG 9 cut(s) 793, 947, 1576, 1612, 2228, 2814, 3879, 4006, 4079
BseRI GAGGAG 2 cut(s) 2107, 3308
BseSI GKGCMC 2 cut(s) 2777, 4079
BseYI CCCAGC 3 cut(s) 836, 2573, 4289
BsgI GTGCAG 1 cut(s) 954
Bsh1236I CGCG 1 cut(s) 1642
Bsh1285I CGRYCG 1 cut(s) 71
BshNI GGYRCC 1 cut(s) 1574
BshTI ACCGGT 2 cut(s) 66, 3209
BsiEI CGRYCG 1 cut(s) 71
BsiHKAI GWGCWC 4 cut(s) 228, 470, 1535, 2652
BsiHKCI CYCGRG 1 cut(s) 2280
BsiSI CCGG 5 cut(s) 58, 67, 334, 3210, 3426
BsiWI CGTACG 1 cut(s) 1110
BslFI GGGAC 2 cut(s) 1993, 3333
BslI CCNNNNNNNGG 9 cut(s) 506, 735, 1073, 2061, 2438, 2747, 3640, 4497, 4728
BsmAI GTCTC 5 cut(s) 1120, 2210, 2475, 4162, 4931
BsmBI CGTCTC 1 cut(s) 1120
BsmFI GGGAC 2 cut(s) 1993, 3333
BsmI GAATGC 2 cut(s) 2948, 5032
BsoBI CYCGRG 1 cut(s) 2280
Bsp119I TTCGAA 1 cut(s) 4105
Bsp120I GGGCCC 1 cut(s) 2773
Bsp1286I GDGCHC 7 cut(s) 228, 470, 1535, 2571, 2652, 2777, 4079
Bsp13I TCCGGA 1 cut(s) 3425
Bsp1720I GCTNAGC 1 cut(s) 2338
BspACI CCGC 5 cut(s) 1772, 2375, 2665, 4998, 5017
BspEI TCCGGA 1 cut(s) 3425
BspFNI CGCG 1 cut(s) 1642
BspHI TCATGA 2 cut(s) 2967, 5045
BspLI GGNNCC 6 cut(s) 332, 1576, 2673, 2775, 2776, 3349
BspPI GGATC 7 cut(s) 223, 1254, 2075, 2666, 2679, 3723, 4400
BspQI GCTCTTC 2 cut(s) 2229, 2640
BspT104I TTCGAA 1 cut(s) 4105
BspT107I GGYRCC 1 cut(s) 1574
BspTI CTTAAG 2 cut(s) 325, 620
BsrDI GCAATG 4 cut(s) 882, 3634, 4473, 5001
BsrFI RCCGGY 3 cut(s) 57, 66, 3209
BsrI ACTGG 9 cut(s) 793, 947, 1576, 1612, 2228, 2814, 3879, 4006, 4079
BssAI RCCGGY 3 cut(s) 57, 66, 3209
BssECI CCNNGG 3 cut(s) 729, 3000, 3732
BssT1I CCWWGG 1 cut(s) 3732
Bst2UI CCWGG 4 cut(s) 2056, 2669, 2763, 3002
Bst6I CTCTTC 9 cut(s) 2229, 2312, 2640, 3111, 3444, 3554, 4311, 4698, 4791
BstAFI CTTAAG 2 cut(s) 325, 620
BstAPI GCANNNNNTGC 2 cut(s) 635, 4247
BstBAI YACGTR 1 cut(s) 465
BstBI TTCGAA 1 cut(s) 4105
BstENI CCTNNNNNAGG 3 cut(s) 2436, 2745, 4495
BstF5I GGATG 8 cut(s) 42, 127, 951, 1897, 2339, 3533, 3893, 4506
BstFNI CGCG 1 cut(s) 1642
BstH2I RGCGCY 3 cut(s) 931, 3125, 3189
BstHHI GCGC 6 cut(s) 930, 1635, 3124, 3188, 3708, 3801
BstMAI GTCTC 5 cut(s) 1120, 2210, 2475, 4162, 4931
BstMCI CGRYCG 1 cut(s) 71
BstNI CCWGG 4 cut(s) 2056, 2669, 2763, 3002
BstPAI GACNNNNGTC 1 cut(s) 1664
BstSCI CCNGG 4 cut(s) 2054, 2667, 2761, 3000
BstSFI CTRYAG 2 cut(s) 4029, 4056
BstSLI GKGCMC 2 cut(s) 2777, 4079
BstUI CGCG 1 cut(s) 1642
BstV2I GAAGAC 1 cut(s) 1212
BstX2I RGATCY 5 cut(s) 1246, 2080, 2671, 3728, 4604
BstXI CCANNNNNNTGG 1 cut(s) 2482
BstYI RGATCY 5 cut(s) 1246, 2080, 2671, 3728, 4604
Bsu36I CCTNAGG 1 cut(s) 2193
BtgZI GCGATG 1 cut(s) 981
BtsCI GGATG 8 cut(s) 42, 127, 951, 1897, 2339, 3533, 3893, 4506
BtsI GCAGTG 2 cut(s) 289, 4833
CaiI CAGNNNCTG 3 cut(s) 3757, 4001, 4940
CciI TCATGA 2 cut(s) 2967, 5045
CfoI GCGC 6 cut(s) 930, 1635, 3124, 3188, 3708, 3801
Cfr10I RCCGGY 3 cut(s) 57, 66, 3209
Cfr13I GGNCC 9 cut(s) 497, 982, 1090, 1774, 2773, 2774, 3217, 3347, 3643
CseI GACGC 2 cut(s) 1654, 1821
Csp6I GTAC 9 cut(s) 524, 950, 1111, 1340, 2938, 3039, 4052, 4733, 4931
CspAI ACCGGT 2 cut(s) 66, 3209
CspCI CAANNNNNGTGG 2 cut(s) 4181, 4216
CviQI GTAC 9 cut(s) 524, 950, 1111, 1340, 2938, 3039, 4052, 4733, 4931
DraI TTTAAA 4 cut(s) 138, 1656, 2134, 4543
EaeI YGGCCR 1 cut(s) 4585
Eam1104I CTCTTC 9 cut(s) 2229, 2312, 2640, 3111, 3444, 3554, 4311, 4698, 4791
EarI CTCTTC 9 cut(s) 2229, 2312, 2640, 3111, 3444, 3554, 4311, 4698, 4791
EciI GGCGGA 1 cut(s) 2654
Eco130I CCWWGG 1 cut(s) 3732
Eco147I AGGCCT 1 cut(s) 4901
Eco24I GRGCYC 2 cut(s) 2571, 2777
Eco32I GATATC 2 cut(s) 1717, 3301
Eco47I GGWCC 5 cut(s) 497, 1090, 1774, 3217, 3347
Eco47III AGCGCT 1 cut(s) 3187
Eco57I CTGAAG 5 cut(s) 261, 2426, 2664, 3468, 3843
Eco72I CACGTG 1 cut(s) 465
Eco81I CCTNAGG 1 cut(s) 2193
Eco88I CYCGRG 1 cut(s) 2280
EcoNI CCTNNNNNAGG 3 cut(s) 2436, 2745, 4495
EcoO109I RGGNCCY 1 cut(s) 2774
EcoRI GAATTC 3 cut(s) 416, 3159, 4383
EcoRII CCWGG 4 cut(s) 2054, 2667, 2761, 3000
EcoRV GATATC 2 cut(s) 1717, 3301
EcoT14I CCWWGG 1 cut(s) 3732
EcoT22I ATGCAT 2 cut(s) 1363, 2878
EcoT38I GRGCYC 2 cut(s) 2571, 2777
ErhI CCWWGG 1 cut(s) 3732
Esp3I CGTCTC 1 cut(s) 1120
FalI AAGNNNNNCTT 8 cut(s) 3048, 3080, 3912, 3944, 4261, 4293, 4544, 4576
FaqI GGGAC 2 cut(s) 1993, 3333
FauNDI CATATG 5 cut(s) 1352, 1756, 2086, 2298, 4351
FbaI TGATCA 2 cut(s) 3102, 3268
FblI GTMKAC 2 cut(s) 4222, 4400
FokI GGATG 8 cut(s) 49, 134, 958, 1904, 2346, 3520, 3880, 4513
FriOI GRGCYC 2 cut(s) 2571, 2777
FspBI CTAG 8 cut(s) 663, 735, 1395, 1473, 3093, 3156, 3224, 3234
FspI TGCGCA 1 cut(s) 3707
GlaI GCGC 6 cut(s) 929, 1634, 3123, 3187, 3707, 3800
GsaI CCCAGC 3 cut(s) 840, 2577, 4293
GsuI CTGGAG 2 cut(s) 2211, 4023
HaeII RGCGCY 3 cut(s) 931, 3125, 3189
HapII CCGG 5 cut(s) 58, 67, 334, 3210, 3426
HgaI GACGC 2 cut(s) 1654, 1821
HhaI GCGC 6 cut(s) 930, 1635, 3124, 3188, 3708, 3801
Hin6I GCGC 6 cut(s) 928, 1633, 3122, 3186, 3706, 3799
HinP1I GCGC 6 cut(s) 928, 1633, 3122, 3186, 3706, 3799
HincII GTYRAC 5 cut(s) 594, 1749, 2634, 4098, 4223
HindII GTYRAC 5 cut(s) 594, 1749, 2634, 4098, 4223
HindIII AAGCTT 6 cut(s) 371, 1259, 2408, 3762, 3926, 4558
HpaI GTTAAC 1 cut(s) 4098
HpaII CCGG 5 cut(s) 58, 67, 334, 3210, 3426
HphI GGTGA 5 cut(s) 56, 1383, 1817, 2420, 4073
Hpy99I CGWCG 1 cut(s) 1815
HspAI GCGC 6 cut(s) 928, 1633, 3122, 3186, 3706, 3799
Kpn2I TCCGGA 1 cut(s) 3425
Ksp22I TGATCA 2 cut(s) 3102, 3268
KspAI GTTAAC 1 cut(s) 4098
LguI GCTCTTC 2 cut(s) 2229, 2640
LmnI GCTCC 6 cut(s) 1279, 1301, 2105, 2741, 4462, 5026
MaeI CTAG 8 cut(s) 663, 735, 1395, 1473, 3093, 3156, 3224, 3234
MflI RGATCY 5 cut(s) 1246, 2080, 2671, 3728, 4604
MhlI GDGCHC 7 cut(s) 228, 470, 1535, 2571, 2652, 2777, 4079
MluI ACGCGT 1 cut(s) 1640
MlyI GAGTC 6 cut(s) 100, 653, 753, 1781, 1975, 2965
MmeI TCCRAC 5 cut(s) 1440, 1874, 2488, 3550, 4641
Mph1103I ATGCAT 2 cut(s) 1363, 2878
MroI TCCGGA 1 cut(s) 3425
MroXI GAANNNNTTC 5 cut(s) 353, 1081, 2715, 3060, 3381
MslI CAYNNNNRTG 4 cut(s) 1706, 1803, 2480, 4233
MspA1I CMGCKG 3 cut(s) 1528, 2424, 5009
MspCI CTTAAG 2 cut(s) 325, 620
MspI CCGG 5 cut(s) 58, 67, 334, 3210, 3426
MspR9I CCNGG 4 cut(s) 2056, 2669, 2763, 3002
Mva1269I GAATGC 2 cut(s) 2948, 5032
MvaI CCWGG 4 cut(s) 2056, 2669, 2763, 3002
MvnI CGCG 1 cut(s) 1642
NdeI CATATG 5 cut(s) 1352, 1756, 2086, 2298, 4351
NlaIV GGNNCC 6 cut(s) 332, 1576, 2673, 2775, 2776, 3349
NmuCI GTSAC 5 cut(s) 645, 721, 1779, 2426, 2918
NsbI TGCGCA 1 cut(s) 3707
NsiI ATGCAT 2 cut(s) 1363, 2878
NspV TTCGAA 1 cut(s) 4105
OliI CACNNNNGTG 1 cut(s) 1706
PaeI GCATGC 4 cut(s) 2040, 3706, 3725, 4469
PagI TCATGA 2 cut(s) 2967, 5045
PceI AGGCCT 1 cut(s) 4901
PciI ACATGT 2 cut(s) 2788, 4217
PciSI GCTCTTC 2 cut(s) 2229, 2640
PctI GAATGC 2 cut(s) 2948, 5032
PdmI GAANNNNTTC 5 cut(s) 353, 1081, 2715, 3060, 3381
PfeI GAWTC 9 cut(s) 773, 1824, 2357, 2627, 3230, 3524, 3551, 3754, 4274
Pfl23II CGTACG 1 cut(s) 1110
PflMI CCANNNNNTGG 2 cut(s) 1073, 2061
PfoI TCCNGGA 1 cut(s) 2761
PinAI ACCGGT 2 cut(s) 66, 3209
PleI GAGTC 6 cut(s) 99, 652, 752, 1781, 1975, 2965
PmaCI CACGTG 1 cut(s) 465
PmlI CACGTG 1 cut(s) 465
PpsI GAGTC 6 cut(s) 99, 652, 752, 1781, 1975, 2965
Ppu21I YACGTR 1 cut(s) 465
PscI ACATGT 2 cut(s) 2788, 4217
PshAI GACNNNNGTC 1 cut(s) 1664
PshBI ATTAAT 2 cut(s) 2495, 3777
PsiI TTATAA 3 cut(s) 1586, 3626, 4496
Psp1406I AACGTT 1 cut(s) 3867
Psp6I CCWGG 4 cut(s) 2054, 2667, 2761, 3000
PspCI CACGTG 1 cut(s) 465
PspFI CCCAGC 3 cut(s) 836, 2573, 4289
PspGI CCWGG 4 cut(s) 2054, 2667, 2761, 3000
PspLI CGTACG 1 cut(s) 1110
PspN4I GGNNCC 6 cut(s) 332, 1576, 2673, 2775, 2776, 3349
PspOMI GGGCCC 1 cut(s) 2773
PspPI GGNCC 9 cut(s) 497, 982, 1090, 1774, 2773, 2774, 3217, 3347, 3643
PstNI CAGNNNCTG 3 cut(s) 3757, 4001, 4940
PsuI RGATCY 5 cut(s) 1246, 2080, 2671, 3728, 4604
PvuII CAGCTG 3 cut(s) 1528, 2424, 5009
RsaI GTAC 9 cut(s) 525, 951, 1112, 1341, 2939, 3040, 4053, 4734, 4932
RsaNI GTAC 9 cut(s) 524, 950, 1111, 1340, 2938, 3039, 4052, 4733, 4931
RseI CAYNNNNRTG 4 cut(s) 1706, 1803, 2480, 4233
SalI GTCGAC 1 cut(s) 4221
SapI GCTCTTC 2 cut(s) 2229, 2640
Sau96I GGNCC 9 cut(s) 497, 982, 1090, 1774, 2773, 2774, 3217, 3347, 3643
ScaI AGTACT 1 cut(s) 2939
SchI GAGTC 6 cut(s) 100, 653, 753, 1781, 1975, 2965
ScrFI CCNGG 4 cut(s) 2056, 2669, 2763, 3002
SduI GDGCHC 7 cut(s) 228, 470, 1535, 2571, 2652, 2777, 4079
SfcI CTRYAG 2 cut(s) 4029, 4056
SfiI GGCCNNNNNGGCC 1 cut(s) 989
SfuI TTCGAA 1 cut(s) 4105
SinI GGWCC 5 cut(s) 497, 1090, 1774, 3217, 3347
SmiMI CAYNNNNRTG 4 cut(s) 1706, 1803, 2480, 4233
SphI GCATGC 4 cut(s) 2040, 3706, 3725, 4469
SseBI AGGCCT 1 cut(s) 4901
SsiI CCGC 5 cut(s) 1772, 2375, 2665, 4998, 5017
SspI AATATT 4 cut(s) 1869, 2493, 2686, 4246
SspMI CTAG 8 cut(s) 663, 735, 1395, 1473, 3093, 3156, 3224, 3234
StuI AGGCCT 1 cut(s) 4901
StyD4I CCNGG 4 cut(s) 2054, 2667, 2761, 3000
StyI CCWWGG 1 cut(s) 3732
TaqI TCGA 8 cut(s) 1038, 1810, 3253, 3322, 3554, 4105, 4222, 4896
TaqII GACCGA 1 cut(s) 192
TatI WGTACW 2 cut(s) 2937, 3038
TauI GCSGC 2 cut(s) 5000, 5020
TfiI GAWTC 9 cut(s) 773, 1824, 2357, 2627, 3230, 3524, 3551, 3754, 4274
TseFI GTSAC 5 cut(s) 645, 721, 1779, 2426, 2918
Tsp45I GTSAC 5 cut(s) 645, 721, 1779, 2426, 2918
Van91I CCANNNNNTGG 2 cut(s) 1073, 2061
Vha464I CTTAAG 2 cut(s) 325, 620
VpaK11BI GGWCC 5 cut(s) 497, 1090, 1774, 3217, 3347
VspI ATTAAT 2 cut(s) 2495, 3777
XagI CCTNNNNNAGG 3 cut(s) 2436, 2745, 4495
XbaI TCTAGA 1 cut(s) 3155
XcmI CCANNNNNNNNNTGG 1 cut(s) 4108
XmiI GTMKAC 2 cut(s) 4222, 4400
XmnI GAANNNNTTC 5 cut(s) 353, 1081, 2715, 3060, 3381
XspI CTAG 8 cut(s) 663, 735, 1395, 1473, 3093, 3156, 3224, 3234
ZrmI AGTACT 1 cut(s) 2939
Zsp2I ATGCAT 2 cut(s) 1363, 2878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.