Rw1G037720

DNA-directed primase polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001804
Physical Location & Seq
Forward (+)
65327013 .. 65330847
3835 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G037720.1

Sequence Viewer

Length: 1539 bp
ATGGACGACGTTGATCGGTTGTTCGAGTGCTTCAAGTGCGGCATCTCACCACCACCATCTGCTTTGAGAGAGAGAAAGAGAATCAAGAGGCAATCGATTCAGAGGAGTAAAGCCCAGGAAGTGGAATCTCCGCGGTCGCCGGAGCAGATAAAGAAAATCGCATCAGATTTACAGCTTTCTGCAGAAAAACTTGTTCAGGGTGGTTCTACAACAGTCGAAGCAAAAAGCTTTAATCGGAGGAAGCAGTTATCTCCGGTTGTATTTTATGGGTCTCCACATGGTGTACCTCCAAAGCAACCATCAAGGTTGTTGCTGCGAGTGTTACGTGAGATACGTGTTGATCTCACTGCACAGAAACGATTGAGCTCAAGGAAGGAAGTATGGGCTACATTTCCAAGGCAGGAGGAAGCAATGCAGTTTGCCAAACGGCACGAGAATGTTCACGTTTTTAGTTATCAAGATCACTTTAGTGGACAAAGAAGGTTCCTTGTTTCAAGTTATAATGAGTTTTGGAGAAGGTACAAGATTATGGAGTCCAAGTTTCGGCACCATTACGAAGTCATTCAGGAGGGTTTACCATGCCACCTTTATTTTGACCTGGAGTTCAATAAGAAAGACAATGGAAACAGAAATGGAGATGAAATGGTTGATCTTTTAATATCAGTTGTTTTTGAAGCCTTGCTTGAAAAGTATTCTATTCAGGGGAACATGGAATGGATATTGGAACTTGATTCCTCTACTGAAGCAAAGTTCTCTCGACATTTAATTATTCGCATTCAGAAGACTGCTTTTAAGGACAACTCACATGTAGGTGCATTTGTCACCGAGATATGCTCACGGATATCAAGTGCAAAAGGGAGGGATGGAAGATTTGAGAATTTGTTTATTAGAAAAGATTCAAGCTCTGCAGACTTTAGTCACCTATTTGTGGACACTGCTGTGTATACCAGGAATCGTTGTTTTCGTTTAGCTCTATCATCAAAGGCAGGGAAGAATTCAGTGCTTCTACCTACTGGGCGCTTCAAAGCTAAGGACATGATGCAGTCTGAAGAGGAGATGTTCATGGCGTCTTTGATCTGCAATTTGGAAGTTGATTGTGAGAAGCTTCTAGTCTGCAAATCTGATCTTGATTGTATAAAGACCCTGCATTTTGATACAGAGGTCAACCCTAGTTTTGGAAAATCTTACAGTTGGCCCCAAGAATTTGCATTGAATGGTTGCACCAGTGATGCTTCAGCAACATACTTCCTGGGGAAATCTCCATTTGCAGCTTTGGATGCATTTGTAGAATTTGTTGCTACAATTGGAAATGTCTCAGGTAAAATTCGCAGCTGGTATTGGTTCTCAGAGTTTGGACTCATGGTCTACAGCATGTCAAGAAACAGATACTGTGAGCGAATTGGCAGACAGCACAAAAGCAATCATGTGATATACATTGCTGACCTCAGAAAGGGTGTTTATTATCAGAAATGTCATGATCCCGACTGCAGAGGTGAGGAAAAGTTAATATCGTTTATCTACTTTGGCTCTCTCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Herpes_UL52

Protein Analysis

512

Amino Acids

59.23

Weight (kDa)

9.08

Isoelectric Point (pI)

46.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Herpes_UL52 PF03121 447 - 499 2.5e-11 Herpesviridae UL52/UL70 DNA primase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 501
AccB1I GGYRCC 1 cut(s) 546
AccB7I CCANNNNNTGG 1 cut(s) 121
AccI GTMKAC 2 cut(s) 944, 1365
AccII CGCG 1 cut(s) 133
AciI CCGC 3 cut(s) 39, 131, 133
AclWI GGATC 1 cut(s) 1472
AcsI RAATTY 5 cut(s) 877, 994, 1202, 1289, 1323
AcuI CTGAAG 3 cut(s) 762, 1068, 1218
AcyI GRCGYC 1 cut(s) 1067
AdeI CACNNNGTG 1 cut(s) 281
AfaI GTAC 2 cut(s) 285, 521
AfiI CCNNNNNNNGG 5 cut(s) 121, 543, 928, 1175, 1450
AflIII ACRYGT 2 cut(s) 334, 805
AgsI TTSAA 8 cut(s) 34, 495, 607, 674, 686, 900, 1024, 1213
AhdI GACNNNNNGTC 1 cut(s) 1361
AjnI CCWGG 4 cut(s) 114, 597, 947, 1248
AleI CACNNNNGTG 2 cut(s) 468, 938
AluBI AGCT 9 cut(s) 175, 228, 366, 903, 971, 1028, 1105, 1271, 1332
AluI AGCT 9 cut(s) 175, 228, 366, 903, 971, 1028, 1105, 1271, 1332
Alw21I GWGCWC 1 cut(s) 368
Alw26I GTCTC 2 cut(s) 276, 1318
AlwI GGATC 1 cut(s) 1472
AlwNI CAGNNNCTG 1 cut(s) 1389
AoxI GGCC 1 cut(s) 1193
ApeKI GCWGC 3 cut(s) 313, 1268, 1329
ApoI RAATTY 5 cut(s) 877, 994, 1202, 1289, 1323
Asp700I GAANNNNTTC 2 cut(s) 561, 895
AspLEI GCGC 1 cut(s) 1020
AspS9I GGNCC 1 cut(s) 1194
AsuHPI GGTGA 4 cut(s) 39, 814, 911, 1505
BaeI ACNNNNGTAYC 2 cut(s) 1146, 1179
BanI GGYRCC 1 cut(s) 546
BanII GRGCYC 1 cut(s) 368
BauI CACGAG 1 cut(s) 431
BbsI GAAGAC 1 cut(s) 788
Bbv12I GWGCWC 1 cut(s) 368
BbvI GCAGC 3 cut(s) 300, 1280, 1341
BccI CCATC 3 cut(s) 64, 307, 857
BceAI ACGGC 1 cut(s) 443
BciT130I CCWGG 4 cut(s) 116, 599, 949, 1250
BcoDI GTCTC 2 cut(s) 276, 1318
BfaI CTAG 2 cut(s) 1109, 1170
BfmI CTRYAG 4 cut(s) 180, 906, 1366, 1486
BfoI RGCGCY 1 cut(s) 1021
BisI GCNGC 4 cut(s) 40, 314, 1269, 1330
BlsI GCNGC 4 cut(s) 41, 315, 1270, 1331
Bme1390I CCNGG 4 cut(s) 116, 599, 949, 1250
BmeRI GACNNNNNGTC 1 cut(s) 1361
BmgT120I GGNCC 1 cut(s) 1194
BmiI GGNNCC 3 cut(s) 485, 548, 1196
BmrFI CCNGG 4 cut(s) 116, 599, 949, 1250
BmrI ACTGGG 1 cut(s) 1023
BmsI GCATC 5 cut(s) 51, 170, 1029, 1219, 1267
BmuI ACTGGG 1 cut(s) 1023
BoxI GACNNNNGTC 1 cut(s) 915
BpiI GAAGAC 1 cut(s) 788
BplI GAGNNNNNCTC 2 cut(s) 818, 850
BpmI CTGGAG 1 cut(s) 620
Bpu10I CCTNAGC 1 cut(s) 1029
BpuEI CTTGAG 1 cut(s) 352
Bsa29I ATCGAT 1 cut(s) 95
BsaAI YACGTR 2 cut(s) 326, 335
BsaHI GRCGYC 1 cut(s) 1067
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 4 cut(s) 114, 131, 395, 1249
BsaWI WCCGGW 1 cut(s) 253
BsaXI ACNNNNNCTCC 2 cut(s) 524, 554
Bsc4I CCNNNNNNNGG 5 cut(s) 121, 543, 928, 1175, 1450
Bse1I ACTGG 2 cut(s) 1018, 1224
Bse3DI GCAATG 2 cut(s) 417, 1434
BseBI CCWGG 4 cut(s) 116, 599, 949, 1250
BseCI ATCGAT 1 cut(s) 95
BseDI CCNNGG 4 cut(s) 114, 131, 395, 1249
BseGI GGATG 2 cut(s) 868, 1282
BseLI CCNNNNNNNGG 5 cut(s) 121, 543, 928, 1175, 1450
BseMI GCAATG 2 cut(s) 417, 1434
BseMII CTCAG 3 cut(s) 1329, 1359, 1459
BseNI ACTGG 2 cut(s) 1018, 1224
BseRI GAGGAG 2 cut(s) 118, 1067
BseXI GCAGC 3 cut(s) 300, 1280, 1341
BsgI GTGCAG 1 cut(s) 333
Bsh1236I CGCG 1 cut(s) 133
Bsh1285I CGRYCG 1 cut(s) 137
BshFI GGCC 1 cut(s) 1195
BshNI GGYRCC 1 cut(s) 546
BshVI ATCGAT 1 cut(s) 95
BsiEI CGRYCG 1 cut(s) 137
BsiHKAI GWGCWC 1 cut(s) 368
BsiSI CCGG 2 cut(s) 140, 254
BslI CCNNNNNNNGG 5 cut(s) 121, 543, 928, 1175, 1450
BsmAI GTCTC 2 cut(s) 276, 1318
BsmI GAATGC 1 cut(s) 774
BsnI GGCC 1 cut(s) 1195
Bso31I GGTCTC 1 cut(s) 276
Bsp1286I GDGCHC 1 cut(s) 368
Bsp143I GATC 7 cut(s) 13, 340, 460, 649, 1074, 1123, 1477
BspACI CCGC 3 cut(s) 39, 131, 133
BspANI GGCC 1 cut(s) 1195
BspCNI CTCAG 3 cut(s) 1328, 1358, 1458
BspDI ATCGAT 1 cut(s) 95
BspFNI CGCG 1 cut(s) 133
BspHI TCATGA 1 cut(s) 1474
BspLI GGNNCC 3 cut(s) 485, 548, 1196
BspMAI CTGCAG 3 cut(s) 184, 910, 1490
BspPI GGATC 1 cut(s) 1472
BspT107I GGYRCC 1 cut(s) 546
BspTNI GGTCTC 1 cut(s) 276
BsrDI GCAATG 2 cut(s) 417, 1434
BsrI ACTGG 2 cut(s) 1018, 1224
BssECI CCNNGG 4 cut(s) 114, 131, 395, 1249
BssMI GATC 7 cut(s) 13, 340, 460, 649, 1074, 1123, 1477
BssNAI GTATAC 1 cut(s) 945
BssNI GRCGYC 1 cut(s) 1067
BssSI CACGAG 1 cut(s) 431
BssT1I CCWWGG 1 cut(s) 395
Bst1107I GTATAC 1 cut(s) 945
Bst2BI CACGAG 1 cut(s) 431
Bst2UI CCWGG 4 cut(s) 116, 599, 949, 1250
Bst4CI ACNGT 3 cut(s) 214, 1190, 1391
Bst6I CTCTTC 1 cut(s) 1044
BstACI GRCGYC 1 cut(s) 1067
BstBAI YACGTR 2 cut(s) 326, 335
BstDEI CTNAG 4 cut(s) 1029, 1315, 1345, 1445
BstDSI CCRYGG 1 cut(s) 131
BstENI CCTNNNNNAGG 1 cut(s) 1448
BstF5I GGATG 2 cut(s) 868, 1282
BstFNI CGCG 1 cut(s) 133
BstH2I RGCGCY 1 cut(s) 1021
BstHHI GCGC 1 cut(s) 1020
BstKTI GATC 7 cut(s) 16, 343, 463, 652, 1077, 1126, 1480
BstMAI GTCTC 2 cut(s) 276, 1318
BstMBI GATC 7 cut(s) 13, 340, 460, 649, 1074, 1123, 1477
BstMCI CGRYCG 1 cut(s) 137
BstMWI GCNNNNNNNGC 2 cut(s) 36, 1277
BstNI CCWGG 4 cut(s) 116, 599, 949, 1250
BstNSI RCATGY 2 cut(s) 809, 1375
BstPAI GACNNNNGTC 1 cut(s) 915
BstSCI CCNGG 4 cut(s) 114, 597, 947, 1248
BstSFI CTRYAG 4 cut(s) 180, 906, 1366, 1486
BstUI CGCG 1 cut(s) 133
BstV1I GCAGC 3 cut(s) 300, 1280, 1341
BstV2I GAAGAC 1 cut(s) 788
BstZ17I GTATAC 1 cut(s) 945
Bsu15I ATCGAT 1 cut(s) 95
BsuRI GGCC 1 cut(s) 1195
BsuTUI ATCGAT 1 cut(s) 95
BtgI CCRYGG 1 cut(s) 131
BtsCI GGATG 2 cut(s) 868, 1282
BtsI GCAGTG 2 cut(s) 345, 933
BtsIMutI CAGTG 4 cut(s) 345, 933, 1005, 1231
CaiI CAGNNNCTG 1 cut(s) 1389
CciI TCATGA 1 cut(s) 1474
CfoI GCGC 1 cut(s) 1020
Cfr13I GGNCC 1 cut(s) 1194
Cfr42I CCGCGG 1 cut(s) 134
ClaI ATCGAT 1 cut(s) 95
CseI GACGC 1 cut(s) 1056
Csp6I GTAC 2 cut(s) 284, 520
CviQI GTAC 2 cut(s) 284, 520
DdeI CTNAG 4 cut(s) 1029, 1315, 1345, 1445
DpnI GATC 7 cut(s) 15, 342, 462, 651, 1076, 1125, 1479
DpnII GATC 7 cut(s) 13, 340, 460, 649, 1074, 1123, 1477
DraIII CACNNNGTG 1 cut(s) 281
DriI GACNNNNNGTC 1 cut(s) 1361
Eam1104I CTCTTC 1 cut(s) 1044
Eam1105I GACNNNNNGTC 1 cut(s) 1361
EarI CTCTTC 1 cut(s) 1044
Ecl136II GAGCTC 1 cut(s) 366
Eco130I CCWWGG 1 cut(s) 395
Eco24I GRGCYC 1 cut(s) 368
Eco31I GGTCTC 1 cut(s) 276
Eco32I GATATC 1 cut(s) 843
Eco53kI GAGCTC 1 cut(s) 366
Eco57I CTGAAG 3 cut(s) 762, 1068, 1218
EcoICRI GAGCTC 1 cut(s) 366
EcoNI CCTNNNNNAGG 1 cut(s) 1448
EcoRI GAATTC 1 cut(s) 994
EcoRII CCWGG 4 cut(s) 114, 597, 947, 1248
EcoRV GATATC 1 cut(s) 843
EcoT14I CCWWGG 1 cut(s) 395
EcoT22I ATGCAT 1 cut(s) 1282
EcoT38I GRGCYC 1 cut(s) 368
ErhI CCWWGG 1 cut(s) 395
FalI AAGNNNNNCTT 2 cut(s) 666, 698
FblI GTMKAC 2 cut(s) 944, 1365
Fnu4HI GCNGC 4 cut(s) 40, 314, 1269, 1330
FokI GGATG 2 cut(s) 875, 1289
FriOI GRGCYC 1 cut(s) 368
Fsp4HI GCNGC 4 cut(s) 40, 314, 1269, 1330
FspBI CTAG 2 cut(s) 1109, 1170
GlaI GCGC 1 cut(s) 1019
GluI GCNGC 4 cut(s) 40, 314, 1269, 1330
GsuI CTGGAG 1 cut(s) 620
HaeII RGCGCY 1 cut(s) 1021
HaeIII GGCC 1 cut(s) 1195
HapII CCGG 2 cut(s) 140, 254
HgaI GACGC 1 cut(s) 1056
HhaI GCGC 1 cut(s) 1020
Hin1I GRCGYC 1 cut(s) 1067
Hin6I GCGC 1 cut(s) 1018
HinP1I GCGC 1 cut(s) 1018
HincII GTYRAC 1 cut(s) 1165
HindII GTYRAC 1 cut(s) 1165
HindIII AAGCTT 2 cut(s) 226, 1103
HinfI GANTC 8 cut(s) 81, 97, 125, 533, 731, 896, 952, 1356
HpaII CCGG 2 cut(s) 140, 254
HphI GGTGA 4 cut(s) 39, 814, 911, 1505
Hpy166II GTNNAC 8 cut(s) 284, 442, 473, 575, 931, 945, 1165, 1366
Hpy188I TCNGA 9 cut(s) 102, 166, 237, 780, 1048, 1123, 1348, 1448, 1467
Hpy188III TCNNGA 8 cut(s) 85, 458, 566, 756, 1127, 1377, 1475, 1481
Hpy8I GTNNAC 8 cut(s) 284, 442, 473, 575, 931, 945, 1165, 1366
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 3 cut(s) 367, 474, 510
HpyCH4III ACNGT 3 cut(s) 214, 1190, 1391
HpyCH4IV ACGT 4 cut(s) 9, 325, 334, 444
HpyF10VI GCNNNNNNNGC 2 cut(s) 36, 1277
HpyF3I CTNAG 4 cut(s) 1029, 1315, 1345, 1445
HpySE526I ACGT 4 cut(s) 9, 325, 334, 444
Hsp92I GRCGYC 1 cut(s) 1067
HspAI GCGC 1 cut(s) 1018
KspI CCGCGG 1 cut(s) 134
Kzo9I GATC 7 cut(s) 13, 340, 460, 649, 1074, 1123, 1477
LmnI GCTCC 1 cut(s) 142
Lsp1109I GCAGC 3 cut(s) 300, 1280, 1341
LweI GCATC 5 cut(s) 51, 170, 1029, 1219, 1267
MaeI CTAG 2 cut(s) 1109, 1170
MaeII ACGT 4 cut(s) 9, 325, 334, 444
MaeIII GTNAC 3 cut(s) 321, 820, 917
MalI GATC 7 cut(s) 15, 342, 462, 651, 1076, 1125, 1479
MboI GATC 7 cut(s) 13, 340, 460, 649, 1074, 1123, 1477
MboII GAAGA 4 cut(s) 793, 879, 1003, 1061
MfeI CAATTG 1 cut(s) 1302
MhlI GDGCHC 1 cut(s) 368
MluCI AATT 9 cut(s) 765, 877, 994, 1081, 1202, 1289, 1302, 1323, 1398
MlyI GAGTC 2 cut(s) 542, 1350
Mph1103I ATGCAT 1 cut(s) 1282
MroXI GAANNNNTTC 2 cut(s) 561, 895
MseI TTAA 5 cut(s) 231, 656, 764, 792, 1505
MslI CAYNNNNRTG 4 cut(s) 435, 468, 810, 938
MspA1I CMGCKG 2 cut(s) 133, 1332
MspI CCGG 2 cut(s) 140, 254
MspR9I CCNGG 4 cut(s) 116, 599, 949, 1250
MunI CAATTG 1 cut(s) 1302
Mva1269I GAATGC 1 cut(s) 774
MvaI CCWGG 4 cut(s) 116, 599, 949, 1250
MvnI CGCG 1 cut(s) 133
MwoI GCNNNNNNNGC 2 cut(s) 36, 1277
NdeII GATC 7 cut(s) 13, 340, 460, 649, 1074, 1123, 1477
NlaIV GGNNCC 3 cut(s) 485, 548, 1196
NmuCI GTSAC 2 cut(s) 820, 917
NsiI ATGCAT 1 cut(s) 1282
NspI RCATGY 2 cut(s) 809, 1375
OliI CACNNNNGTG 2 cut(s) 468, 938
PagI TCATGA 1 cut(s) 1474
PciI ACATGT 1 cut(s) 805
PcsI WCGNNNNNNNCGW 2 cut(s) 331, 961
PctI GAATGC 1 cut(s) 774
PdmI GAANNNNTTC 2 cut(s) 561, 895
PfeI GAWTC 6 cut(s) 81, 97, 125, 731, 896, 952
PflMI CCANNNNNTGG 1 cut(s) 121
PkrI GCNGC 4 cut(s) 41, 315, 1270, 1331
PleI GAGTC 2 cut(s) 541, 1350
PpsI GAGTC 2 cut(s) 541, 1350
Ppu21I YACGTR 2 cut(s) 326, 335
PscI ACATGT 1 cut(s) 805
PshAI GACNNNNGTC 1 cut(s) 915
PsiI TTATAA 1 cut(s) 501
Psp124BI GAGCTC 1 cut(s) 368
Psp6I CCWGG 4 cut(s) 114, 597, 947, 1248
PspGI CCWGG 4 cut(s) 114, 597, 947, 1248
PspN4I GGNNCC 3 cut(s) 485, 548, 1196
PspPI GGNCC 1 cut(s) 1194
PstI CTGCAG 3 cut(s) 184, 910, 1490
PstNI CAGNNNCTG 1 cut(s) 1389
PvuII CAGCTG 1 cut(s) 1332
RsaI GTAC 2 cut(s) 285, 521
RsaNI GTAC 2 cut(s) 284, 520
RseI CAYNNNNRTG 4 cut(s) 435, 468, 810, 938
SacI GAGCTC 1 cut(s) 368
SacII CCGCGG 1 cut(s) 134
SaqAI TTAA 5 cut(s) 231, 656, 764, 792, 1505
SatI GCNGC 4 cut(s) 40, 314, 1269, 1330
Sau3AI GATC 7 cut(s) 13, 340, 460, 649, 1074, 1123, 1477
Sau96I GGNCC 1 cut(s) 1194
SchI GAGTC 2 cut(s) 542, 1350
ScrFI CCNGG 4 cut(s) 116, 599, 949, 1250
SduI GDGCHC 1 cut(s) 368
SfaNI GCATC 5 cut(s) 51, 170, 1029, 1219, 1267
SfcI CTRYAG 4 cut(s) 180, 906, 1366, 1486
Sfr303I CCGCGG 1 cut(s) 134
SgrBI CCGCGG 1 cut(s) 134
SmiMI CAYNNNNRTG 4 cut(s) 435, 468, 810, 938
SmlI CTYRAG 1 cut(s) 367
SmoI CTYRAG 1 cut(s) 367
Sse9I AATT 9 cut(s) 765, 877, 994, 1081, 1202, 1289, 1302, 1323, 1398
SsiI CCGC 3 cut(s) 39, 131, 133
SspMI CTAG 2 cut(s) 1109, 1170
SstI GAGCTC 1 cut(s) 368
StyD4I CCNGG 4 cut(s) 114, 597, 947, 1248
StyI CCWWGG 1 cut(s) 395
TaaI ACNGT 3 cut(s) 214, 1190, 1391
TaiI ACGT 4 cut(s) 12, 328, 337, 447
TaqI TCGA 4 cut(s) 24, 95, 216, 757
TasI AATT 9 cut(s) 765, 877, 994, 1081, 1202, 1289, 1302, 1323, 1398
TauI GCSGC 1 cut(s) 42
TfiI GAWTC 6 cut(s) 81, 97, 125, 731, 896, 952
Tru1I TTAA 5 cut(s) 231, 656, 764, 792, 1505
Tru9I TTAA 5 cut(s) 231, 656, 764, 792, 1505
TscAI CASTG 4 cut(s) 352, 940, 1005, 1231
TseFI GTSAC 2 cut(s) 820, 917
TseI GCWGC 3 cut(s) 313, 1268, 1329
Tsp45I GTSAC 2 cut(s) 820, 917
TspDTI ATGAA 2 cut(s) 654, 1051
TspGWI ACGGA 1 cut(s) 853
TspRI CASTG 4 cut(s) 352, 940, 1005, 1231
Van91I CCANNNNNTGG 1 cut(s) 121
XagI CCTNNNNNAGG 1 cut(s) 1448
XapI RAATTY 5 cut(s) 877, 994, 1202, 1289, 1323
XceI RCATGY 2 cut(s) 809, 1375
XmiI GTMKAC 2 cut(s) 944, 1365
XmnI GAANNNNTTC 2 cut(s) 561, 895
XspI CTAG 2 cut(s) 1109, 1170
Zsp2I ATGCAT 1 cut(s) 1282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.