MD01G1209800.v1.1

DNA-directed primase polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001804
Physical Location & Seq
Reverse (-)
30392536 .. 30396611
4076 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1209800.v1.1.491

Sequence Viewer

Length: 1764 bp
ATGGACGACGTCGATCGGTTGTTCGAGTGCTTCAAGTGCGGCATCTCTCCTCCTCAATCCGCATTGAGGGAAAGGAAGAGAAGCAAAAGCGATTTGAAGCGGAGGAGTAAAACCCCGGAAGTCTCCTCTCCAGGCTCAGCAGAGAACACAAAGAAAAATACATCAGAATTACAACTTTCCGCCGACAAGTCTGGTCCTACAAGTGTTAAACCAAACAAGTTTAATCGTCAAAAGGAGTTTTCCCCAGTTGTATTCTACGGATCTCCACATGGTGTGCCCCCCAAAAAACCATCAAGGTCGTTTCTGCGACTGCTACGTGAAATACGTGTTGATCTCGCTGCACAAAAAAGATTGACTTCAAGGAAGGAAGTGTGGACTACGTTTCCAAGGCAGGATGAAGCAATGCAGTTTGTCAAAGGGCATGAGAATGTTCATGTATTTAGTTATCAAGATCATTATAGTGGACAGAGAAGGTTCCTCGTTTCAACATATAACGATTTTTGGAGAAGGTATAAAAATATGAATCCCAGGTTCCGTCACCACTACGAAGTGATCCAGGAGGGTTTGCCGTGTCACCTTTATTTTGACTTGGAATTCAGTAGAAGGGACAATGCAGACAGAAATGGAGATGAAATGGTTGACCTCTTGATATCAGCCATTTTTGAAGCCCTGCTTGAAAAGTATTCTATTATCGGAAACAAGGAATGGATATTGGAGCTTGATTCCTCTAATGAAGCAAAGTTCTCTCGTCACCTAATCATTCGAATAGAAAAGACTGCTTTTAAGGACAACTCACATGCAGGTGCATTTGTCACTGAGATATGCTCACGGATTTCAAGTGCAAAGGAGAGAGATGGAAGATTTGGAAACTTATTTATCAGAAAAGATTCAAGCTCCTCTGACTCCCCTAGTAAACTATTTGTGGACACTGCTGTATATACTCGAAATCGTTGCTTTCGTCTAGCTCTATCATCAAAGGCAGGGAAGAATTCAGTGCTTCTGCCTACTGGGCGTTTCAAAGCCAACAAGATGTGTGAGGAGGAGATGTTCATGGCATCCTTGATCTGCAATTTGGATGTTGATTGTGAAAAGCTTCTAGTATGCAAACCAGATCTTGATTGTATAAAGACTCTGCATTTTGACACCGAGGTAAACCGTGGTCTTGGAAAATGTTACAGTTGGCCCCAAGAATATGCATTGAATGGTTGCACAAGTGGTGCTTCAGCAACATACTTCCTAGGCAAATCTCCATTTCCAGCTTTGGATGCATTTGTAGAATCTGTTGCCACCATTGGAAATGTATCAGGTAAAATTCGGAGCTGGTATTGGCTTTCAGAGTTTGCACTCATGGTCTACAGCATGTCAAGAAATAGATACTGTGAGCGAATTGGCAGACAGCATAAAAGCAATCACGTGATATACGTTGCCGACCTCAGAAGGGCTTCTTATTATCAGAAATGTCATGATCCTGACTGCAGAGGTTATCGTTCTGCCTCGCGACCAATTCCATGGGAAATCATCCCTGACATGGTGTATGAATATAAAAATGATCCTGAATCTGTTCTCTATGGTGATGAGAAAATGAGCAGCAGTTGCGTTACAGATTCATGGTGGCGTGAAGCCATTAGAGTTGCAGATGATGTCGAGAATAAGCAAAATTCTTTAGGGATCAGTAATATGGAAAACATTGATTCCGATGATGAAGACTGGTGGATGGCTGTGGAAAGGACTGCATCCCAAGCTGAATTAGCGCATGTCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Herpes_UL52

Protein Analysis

588

Amino Acids

67.64

Weight (kDa)

8.53

Isoelectric Point (pI)

49.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Herpes_UL52 PF03121 452 - 504 3.1e-12 Herpesviridae UL52/UL70 DNA primase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 791
AatII GACGTC 1 cut(s) 12
Acc36I ACCTGC 1 cut(s) 791
AccI GTMKAC 1 cut(s) 1353
AccII CGCG 1 cut(s) 1498
AciI CCGC 4 cut(s) 39, 60, 100, 180
AclWI GGATC 5 cut(s) 268, 547, 1460, 1544, 1676
AcsI RAATTY 4 cut(s) 593, 988, 1311, 1657
AcuI CTGAAG 1 cut(s) 1206
AcvI CACGTG 1 cut(s) 1414
AcyI GRCGYC 1 cut(s) 9
AdeI CACNNNGTG 1 cut(s) 272
AfiI CCNNNNNNNGG 3 cut(s) 66, 1438, 1528
AflIII ACRYGT 1 cut(s) 325
AjnI CCWGG 3 cut(s) 130, 527, 555
AloI GAACNNNNNNTCC 2 cut(s) 1031, 1063
AluBI AGCT 7 cut(s) 718, 894, 965, 1093, 1259, 1320, 1742
AluI AGCT 7 cut(s) 718, 894, 965, 1093, 1259, 1320, 1742
Alw26I GTCTC 1 cut(s) 127
AlwI GGATC 5 cut(s) 268, 547, 1460, 1544, 1676
AoxI GGCC 1 cut(s) 1181
ApeKI GCWGC 2 cut(s) 338, 1587
ApoI RAATTY 4 cut(s) 593, 988, 1311, 1657
Asp700I GAANNNNTTC 4 cut(s) 886, 1092, 1441, 1560
AspA2I CCTAGG 1 cut(s) 1237
AspLEI GCGC 1 cut(s) 1753
AspS9I GGNCC 2 cut(s) 194, 1182
AsuC2I CCSGG 1 cut(s) 116
AsuHPI GGTGA 4 cut(s) 530, 566, 743, 1583
AsuII TTCGAA 1 cut(s) 763
AvaII GGWCC 1 cut(s) 194
AvrII CCTAGG 1 cut(s) 1237
BaeGI GKGCMC 1 cut(s) 279
BbrPI CACGTG 1 cut(s) 1414
BbsI GAAGAC 1 cut(s) 1710
BbvI GCAGC 2 cut(s) 325, 1599
BccI CCATC 3 cut(s) 298, 848, 1708
BceAI ACGGC 1 cut(s) 553
BcgI CGANNNNNNTGC 2 cut(s) 933, 967
BciT130I CCWGG 3 cut(s) 132, 529, 557
BcnI CCSGG 1 cut(s) 116
BcoDI GTCTC 1 cut(s) 127
BfaI CTAG 4 cut(s) 909, 962, 1097, 1238
BfmI CTRYAG 2 cut(s) 1354, 1474
BfuAI ACCTGC 1 cut(s) 791
BglI GCCNNNNNGGC 1 cut(s) 1009
BglII AGATCT 1 cut(s) 1111
BisI GCNGC 3 cut(s) 40, 339, 1588
BlnI CCTAGG 1 cut(s) 1237
BlpI GCTNAGC 1 cut(s) 136
BlsI GCNGC 3 cut(s) 41, 340, 1589
Bme1390I CCNGG 4 cut(s) 116, 132, 529, 557
Bme18I GGWCC 1 cut(s) 194
BmgT120I GGNCC 2 cut(s) 194, 1182
BmiI GGNNCC 3 cut(s) 476, 533, 1184
BmrFI CCNGG 4 cut(s) 116, 132, 529, 557
BmrI ACTGGG 2 cut(s) 239, 1017
BmsI GCATC 4 cut(s) 51, 1064, 1255, 1742
BmuI ACTGGG 2 cut(s) 239, 1017
BpiI GAAGAC 1 cut(s) 1710
BplI GAGNNNNNCTC 2 cut(s) 809, 841
BpmI CTGGAG 1 cut(s) 114
Bpu1102I GCTNAGC 1 cut(s) 136
Bpu14I TTCGAA 1 cut(s) 763
BpuMI CCSGG 1 cut(s) 116
BsaAI YACGTR 3 cut(s) 317, 326, 1414
BsaHI GRCGYC 1 cut(s) 9
BsaJI CCNNGG 7 cut(s) 114, 386, 527, 1146, 1156, 1237, 1508
BsaXI ACNNNNNCTCC 2 cut(s) 1031, 1061
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 1438, 1528
Bse1I ACTGG 3 cut(s) 245, 1012, 1712
Bse3DI GCAATG 1 cut(s) 408
BseBI CCWGG 3 cut(s) 132, 529, 557
BseDI CCNNGG 7 cut(s) 114, 386, 527, 1146, 1156, 1237, 1508
BseGI GGATG 7 cut(s) 400, 1055, 1081, 1270, 1518, 1719, 1733
BseLI CCNNNNNNNGG 3 cut(s) 66, 1438, 1528
BseMI GCAATG 1 cut(s) 408
BseMII CTCAG 3 cut(s) 150, 807, 1447
BseNI ACTGG 3 cut(s) 245, 1012, 1712
BseRI GAGGAG 7 cut(s) 39, 42, 115, 118, 886, 1052, 1055
BseSI GKGCMC 1 cut(s) 279
BseXI GCAGC 2 cut(s) 325, 1599
BsgI GTGCAG 1 cut(s) 324
Bsh1236I CGCG 1 cut(s) 1498
Bsh1285I CGRYCG 1 cut(s) 16
BshFI GGCC 1 cut(s) 1183
BsiEI CGRYCG 1 cut(s) 16
BsiSI CCGG 1 cut(s) 116
BslFI GGGAC 1 cut(s) 620
BslI CCNNNNNNNGG 3 cut(s) 66, 1438, 1528
BsmAI GTCTC 1 cut(s) 127
BsmFI GGGAC 1 cut(s) 620
BsnI GGCC 1 cut(s) 1183
Bsp119I TTCGAA 1 cut(s) 763
Bsp1286I GDGCHC 1 cut(s) 279
Bsp1720I GCTNAGC 1 cut(s) 136
Bsp19I CCATGG 1 cut(s) 1508
Bsp68I TCGCGA 1 cut(s) 1498
BspACI CCGC 4 cut(s) 39, 60, 100, 180
BspANI GGCC 1 cut(s) 1183
BspCNI CTCAG 3 cut(s) 149, 808, 1446
BspFNI CGCG 1 cut(s) 1498
BspHI TCATGA 1 cut(s) 1462
BspLI GGNNCC 3 cut(s) 476, 533, 1184
BspMAI CTGCAG 1 cut(s) 1478
BspMI ACCTGC 1 cut(s) 791
BspPI GGATC 5 cut(s) 268, 547, 1460, 1544, 1676
BspT104I TTCGAA 1 cut(s) 763
BsrDI GCAATG 1 cut(s) 408
BsrI ACTGG 3 cut(s) 245, 1012, 1712
BssECI CCNNGG 7 cut(s) 114, 386, 527, 1146, 1156, 1237, 1508
BssNI GRCGYC 1 cut(s) 9
BssT1I CCWWGG 3 cut(s) 386, 1237, 1508
Bst2UI CCWGG 3 cut(s) 132, 529, 557
Bst4CI ACNGT 3 cut(s) 1157, 1178, 1379
Bst6I CTCTTC 1 cut(s) 71
BstACI GRCGYC 1 cut(s) 9
BstAPI GCANNNNNTGC 1 cut(s) 1593
BstBAI YACGTR 3 cut(s) 317, 326, 1414
BstBI TTCGAA 1 cut(s) 763
BstDEI CTNAG 3 cut(s) 136, 816, 1433
BstDSI CCRYGG 2 cut(s) 1156, 1508
BstF5I GGATG 7 cut(s) 400, 1055, 1081, 1270, 1518, 1719, 1733
BstFNI CGCG 1 cut(s) 1498
BstHHI GCGC 1 cut(s) 1753
BstMAI GTCTC 1 cut(s) 127
BstMCI CGRYCG 1 cut(s) 16
BstMWI GCNNNNNNNGC 6 cut(s) 36, 1009, 1265, 1593, 1739, 1748
BstNI CCWGG 3 cut(s) 132, 529, 557
BstNSI RCATGY 3 cut(s) 800, 1363, 1757
BstSCI CCNGG 4 cut(s) 114, 130, 527, 555
BstSFI CTRYAG 2 cut(s) 1354, 1474
BstSLI GKGCMC 1 cut(s) 279
BstUI CGCG 1 cut(s) 1498
BstV1I GCAGC 2 cut(s) 325, 1599
BstV2I GAAGAC 1 cut(s) 1710
BstX2I RGATCY 2 cut(s) 260, 1111
BstXI CCANNNNNNTGG 1 cut(s) 1509
BstYI RGATCY 2 cut(s) 260, 1111
BsuRI GGCC 1 cut(s) 1183
BtgI CCRYGG 2 cut(s) 1156, 1508
BtsCI GGATG 7 cut(s) 400, 1055, 1081, 1270, 1518, 1719, 1733
BtsI GCAGTG 1 cut(s) 927
BtsIMutI CAGTG 3 cut(s) 813, 927, 999
BtuMI TCGCGA 1 cut(s) 1498
BveI ACCTGC 1 cut(s) 791
CciI TCATGA 1 cut(s) 1462
CfoI GCGC 1 cut(s) 1753
Cfr13I GGNCC 2 cut(s) 194, 1182
DdeI CTNAG 3 cut(s) 136, 816, 1433
DraIII CACNNNGTG 1 cut(s) 272
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
EciI GGCGGA 1 cut(s) 169
Eco130I CCWWGG 3 cut(s) 386, 1237, 1508
Eco32I GATATC 1 cut(s) 651
Eco47I GGWCC 1 cut(s) 194
Eco57I CTGAAG 1 cut(s) 1206
Eco72I CACGTG 1 cut(s) 1414
EcoRI GAATTC 2 cut(s) 593, 988
EcoRII CCWGG 3 cut(s) 130, 527, 555
EcoRV GATATC 1 cut(s) 651
EcoT14I CCWWGG 3 cut(s) 386, 1237, 1508
EcoT22I ATGCAT 2 cut(s) 1198, 1270
ErhI CCWWGG 3 cut(s) 386, 1237, 1508
FalI AAGNNNNNCTT 8 cut(s) 340, 372, 657, 689, 1204, 1236, 1429, 1461
FaqI GGGAC 1 cut(s) 620
FblI GTMKAC 1 cut(s) 1353
Fnu4HI GCNGC 3 cut(s) 40, 339, 1588
FokI GGATG 7 cut(s) 407, 1042, 1088, 1277, 1505, 1720, 1726
Fsp4HI GCNGC 3 cut(s) 40, 339, 1588
FspBI CTAG 4 cut(s) 909, 962, 1097, 1238
GlaI GCGC 1 cut(s) 1752
GluI GCNGC 3 cut(s) 40, 339, 1588
GsuI CTGGAG 1 cut(s) 114
HaeIII GGCC 1 cut(s) 1183
HapII CCGG 1 cut(s) 116
HhaI GCGC 1 cut(s) 1753
Hin1I GRCGYC 1 cut(s) 9
Hin6I GCGC 1 cut(s) 1751
HinP1I GCGC 1 cut(s) 1751
HincII GTYRAC 2 cut(s) 640, 1759
HindII GTYRAC 2 cut(s) 640, 1759
HindIII AAGCTT 1 cut(s) 1091
HinfI GANTC 9 cut(s) 523, 722, 887, 902, 1129, 1277, 1556, 1604, 1691
HpaII CCGG 1 cut(s) 116
HphI GGTGA 4 cut(s) 530, 566, 743, 1583
Hpy166II GTNNAC 8 cut(s) 375, 464, 640, 914, 925, 1153, 1354, 1759
Hpy188I TCNGA 9 cut(s) 166, 695, 881, 901, 1317, 1336, 1436, 1455, 1696
Hpy188III TCNNGA 9 cut(s) 449, 646, 1115, 1365, 1463, 1469, 1497, 1553, 1645
Hpy8I GTNNAC 8 cut(s) 375, 464, 640, 914, 925, 1153, 1354, 1759
Hpy99I CGWCG 2 cut(s) 11, 14
HpyAV CCTTC 5 cut(s) 358, 465, 501, 597, 1431
HpyCH4III ACNGT 3 cut(s) 1157, 1178, 1379
HpyCH4IV ACGT 6 cut(s) 9, 316, 325, 380, 1413, 1422
HpyF10VI GCNNNNNNNGC 6 cut(s) 36, 1009, 1265, 1593, 1739, 1748
HpyF3I CTNAG 3 cut(s) 136, 816, 1433
HpySE526I ACGT 6 cut(s) 9, 316, 325, 380, 1413, 1422
Hsp92I GRCGYC 1 cut(s) 9
HspAI GCGC 1 cut(s) 1751
LmnI GCTCC 3 cut(s) 715, 899, 1317
Lsp1109I GCAGC 2 cut(s) 325, 1599
LweI GCATC 4 cut(s) 51, 1064, 1255, 1742
MaeI CTAG 4 cut(s) 909, 962, 1097, 1238
MaeII ACGT 6 cut(s) 9, 316, 325, 380, 1413, 1422
MaeIII GTNAC 6 cut(s) 536, 572, 749, 811, 1172, 1597
MboII GAAGA 4 cut(s) 88, 870, 997, 1715
MflI RGATCY 2 cut(s) 260, 1111
MhlI GDGCHC 1 cut(s) 279
MluCI AATT 9 cut(s) 167, 593, 988, 1069, 1311, 1386, 1503, 1657, 1745
MlyI GAGTC 2 cut(s) 896, 1123
Mph1103I ATGCAT 2 cut(s) 1198, 1270
MroXI GAANNNNTTC 4 cut(s) 886, 1092, 1441, 1560
MseI TTAA 3 cut(s) 207, 222, 783
MslI CAYNNNNRTG 3 cut(s) 426, 459, 801
MspI CCGG 1 cut(s) 116
MspR9I CCNGG 4 cut(s) 116, 132, 529, 557
MvaI CCWGG 3 cut(s) 132, 529, 557
MvnI CGCG 1 cut(s) 1498
MwoI GCNNNNNNNGC 6 cut(s) 36, 1009, 1265, 1593, 1739, 1748
NciI CCSGG 1 cut(s) 116
NcoI CCATGG 1 cut(s) 1508
NlaIV GGNNCC 3 cut(s) 476, 533, 1184
NmuCI GTSAC 4 cut(s) 536, 572, 749, 811
NruI TCGCGA 1 cut(s) 1498
NsiI ATGCAT 2 cut(s) 1198, 1270
NspI RCATGY 3 cut(s) 800, 1363, 1757
NspV TTCGAA 1 cut(s) 763
PagI TCATGA 1 cut(s) 1462
PaqCI CACCTGC 1 cut(s) 791
PcsI WCGNNNNNNNCGW 3 cut(s) 322, 955, 1419
PdmI GAANNNNTTC 4 cut(s) 886, 1092, 1441, 1560
PfeI GAWTC 7 cut(s) 523, 722, 887, 1277, 1556, 1604, 1691
PflFI GACNNNGTC 1 cut(s) 8
PfoI TCCNGGA 1 cut(s) 555
PkrI GCNGC 3 cut(s) 41, 340, 1589
Ple19I CGATCG 1 cut(s) 16
PleI GAGTC 2 cut(s) 896, 1123
PmaCI CACGTG 1 cut(s) 1414
PmlI CACGTG 1 cut(s) 1414
PpsI GAGTC 2 cut(s) 896, 1123
Ppu21I YACGTR 3 cut(s) 317, 326, 1414
Psp6I CCWGG 3 cut(s) 130, 527, 555
PspCI CACGTG 1 cut(s) 1414
PspGI CCWGG 3 cut(s) 130, 527, 555
PspN4I GGNNCC 3 cut(s) 476, 533, 1184
PspPI GGNCC 2 cut(s) 194, 1182
PstI CTGCAG 1 cut(s) 1478
PsuI RGATCY 2 cut(s) 260, 1111
PsyI GACNNNGTC 1 cut(s) 8
PvuI CGATCG 1 cut(s) 16
RruI TCGCGA 1 cut(s) 1498
RseI CAYNNNNRTG 3 cut(s) 426, 459, 801
SaqAI TTAA 3 cut(s) 207, 222, 783
SatI GCNGC 3 cut(s) 40, 339, 1588
Sau96I GGNCC 2 cut(s) 194, 1182
SchI GAGTC 2 cut(s) 896, 1123
ScrFI CCNGG 4 cut(s) 116, 132, 529, 557
SduI GDGCHC 1 cut(s) 279
SfaNI GCATC 4 cut(s) 51, 1064, 1255, 1742
SfcI CTRYAG 2 cut(s) 1354, 1474
SfuI TTCGAA 1 cut(s) 763
SinI GGWCC 1 cut(s) 194
SmiMI CAYNNNNRTG 3 cut(s) 426, 459, 801
Sse9I AATT 9 cut(s) 167, 593, 988, 1069, 1311, 1386, 1503, 1657, 1745
SsiI CCGC 4 cut(s) 39, 60, 100, 180
SspMI CTAG 4 cut(s) 909, 962, 1097, 1238
StyD4I CCNGG 4 cut(s) 114, 130, 527, 555
StyI CCWWGG 3 cut(s) 386, 1237, 1508
TaaI ACNGT 3 cut(s) 1157, 1178, 1379
TaiI ACGT 6 cut(s) 12, 319, 328, 383, 1416, 1425
TaqI TCGA 5 cut(s) 12, 24, 763, 943, 1644
TasI AATT 9 cut(s) 167, 593, 988, 1069, 1311, 1386, 1503, 1657, 1745
TauI GCSGC 1 cut(s) 42
TfiI GAWTC 7 cut(s) 523, 722, 887, 1277, 1556, 1604, 1691
Tru1I TTAA 3 cut(s) 207, 222, 783
Tru9I TTAA 3 cut(s) 207, 222, 783
TscAI CASTG 3 cut(s) 820, 934, 999
TseFI GTSAC 4 cut(s) 536, 572, 749, 811
TseI GCWGC 2 cut(s) 338, 1587
Tsp45I GTSAC 4 cut(s) 536, 572, 749, 811
TspDTI ATGAA 9 cut(s) 411, 422, 536, 645, 747, 1039, 1551, 1596, 1716
TspGWI ACGGA 3 cut(s) 273, 524, 844
TspRI CASTG 3 cut(s) 820, 934, 999
Tth111I GACNNNGTC 1 cut(s) 8
VpaK11BI GGWCC 1 cut(s) 194
XapI RAATTY 4 cut(s) 593, 988, 1311, 1657
XceI RCATGY 3 cut(s) 800, 1363, 1757
XmaJI CCTAGG 1 cut(s) 1237
XmiI GTMKAC 1 cut(s) 1353
XmnI GAANNNNTTC 4 cut(s) 886, 1092, 1441, 1560
XspI CTAG 4 cut(s) 909, 962, 1097, 1238
ZraI GACGTC 1 cut(s) 10
Zsp2I ATGCAT 2 cut(s) 1198, 1270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.