Belongs to the peptidase A1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 207 |
| Acc36I | ACCTGC | 1 cut(s) | 207 |
| AccII | CGCG | 3 cut(s) | 69, 71, 437 |
| AciI | CCGC | 5 cut(s) | 5, 100, 170, 435, 803 |
| AclWI | GGATC | 2 cut(s) | 1114, 1202 |
| AcoI | YGGCCR | 1 cut(s) | 167 |
| AcsI | RAATTY | 2 cut(s) | 553, 967 |
| AcuI | CTGAAG | 2 cut(s) | 297, 762 |
| AcyI | GRCGYC | 1 cut(s) | 347 |
| AfaI | GTAC | 4 cut(s) | 476, 587, 1046, 1121 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 162, 278, 917, 1274, 1415, 1467 |
| AflII | CTTAAG | 1 cut(s) | 977 |
| AgsI | TTSAA | 4 cut(s) | 492, 994, 1345, 1365 |
| AjnI | CCWGG | 2 cut(s) | 726, 873 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 806, 838 |
| Alw21I | GWGCWC | 4 cut(s) | 67, 182, 1460, 1498 |
| Alw26I | GTCTC | 4 cut(s) | 138, 850, 977, 1569 |
| AlwI | GGATC | 2 cut(s) | 1114, 1202 |
| AoxI | GGCC | 3 cut(s) | 153, 167, 878 |
| ApeKI | GCWGC | 4 cut(s) | 516, 644, 1450, 1514 |
| ApoI | RAATTY | 2 cut(s) | 553, 967 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 821 |
| AspLEI | GCGC | 4 cut(s) | 71, 73, 425, 1421 |
| AspS9I | GGNCC | 4 cut(s) | 539, 871, 878, 1201 |
| AsuC2I | CCSGG | 1 cut(s) | 1464 |
| AsuHPI | GGTGA | 2 cut(s) | 665, 1241 |
| AsuII | TTCGAA | 1 cut(s) | 557 |
| AvaII | GGWCC | 3 cut(s) | 539, 871, 1201 |
| AxyI | CCTNAGG | 1 cut(s) | 157 |
| BanII | GRGCYC | 2 cut(s) | 685, 1498 |
| BauI | CACGAG | 1 cut(s) | 470 |
| BbsI | GAAGAC | 1 cut(s) | 125 |
| Bbv12I | GWGCWC | 4 cut(s) | 67, 182, 1460, 1498 |
| BbvI | GCAGC | 4 cut(s) | 528, 656, 1462, 1501 |
| BccI | CCATC | 5 cut(s) | 218, 490, 725, 740, 1516 |
| BciT130I | CCWGG | 2 cut(s) | 728, 875 |
| BciVI | GTATCC | 1 cut(s) | 370 |
| BclI | TGATCA | 1 cut(s) | 1231 |
| BcnI | CCSGG | 1 cut(s) | 1464 |
| BcoDI | GTCTC | 4 cut(s) | 138, 850, 977, 1569 |
| BfaI | CTAG | 4 cut(s) | 32, 1349, 1403, 1603 |
| BfrI | CTTAAG | 1 cut(s) | 977 |
| BfuAI | ACCTGC | 1 cut(s) | 207 |
| BfuI | GTATCC | 1 cut(s) | 370 |
| BglII | AGATCT | 2 cut(s) | 1144, 1375 |
| BisI | GCNGC | 6 cut(s) | 6, 170, 517, 645, 1451, 1515 |
| BlsI | GCNGC | 6 cut(s) | 7, 171, 518, 646, 1452, 1516 |
| BmcAI | AGTACT | 2 cut(s) | 587, 1121 |
| Bme1390I | CCNGG | 3 cut(s) | 728, 875, 1464 |
| Bme18I | GGWCC | 3 cut(s) | 539, 871, 1201 |
| BmgT120I | GGNCC | 4 cut(s) | 539, 871, 878, 1201 |
| BmiI | GGNNCC | 3 cut(s) | 402, 872, 879 |
| BmrFI | CCNGG | 3 cut(s) | 728, 875, 1464 |
| BmrI | ACTGGG | 1 cut(s) | 390 |
| BmsI | GCATC | 3 cut(s) | 649, 671, 1526 |
| BmuI | ACTGGG | 1 cut(s) | 390 |
| BpiI | GAAGAC | 1 cut(s) | 125 |
| Bpu14I | TTCGAA | 1 cut(s) | 557 |
| BpuMI | CCSGG | 1 cut(s) | 1464 |
| BsaAI | YACGTR | 1 cut(s) | 330 |
| BsaHI | GRCGYC | 1 cut(s) | 347 |
| BsaI | GGTCTC | 1 cut(s) | 138 |
| BsaJI | CCNNGG | 5 cut(s) | 150, 183, 874, 1112, 1462 |
| BsaWI | WCCGGW | 1 cut(s) | 1317 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 89, 119 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 162, 278, 917, 1274, 1415, 1467 |
| Bse118I | RCCGGY | 1 cut(s) | 249 |
| Bse1I | ACTGG | 4 cut(s) | 259, 385, 1005, 1288 |
| Bse21I | CCTNAGG | 1 cut(s) | 157 |
| BseBI | CCWGG | 2 cut(s) | 728, 875 |
| BseDI | CCNNGG | 5 cut(s) | 150, 183, 874, 1112, 1462 |
| BseGI | GGATG | 4 cut(s) | 704, 736, 899, 1535 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 162, 278, 917, 1274, 1415, 1467 |
| BseNI | ACTGG | 4 cut(s) | 259, 385, 1005, 1288 |
| BsePI | GCGCGC | 1 cut(s) | 69 |
| BseRI | GAGGAG | 2 cut(s) | 190, 417 |
| BseXI | GCAGC | 4 cut(s) | 528, 656, 1462, 1501 |
| Bsh1236I | CGCG | 3 cut(s) | 69, 71, 437 |
| BshFI | GGCC | 3 cut(s) | 155, 169, 880 |
| BsiHKAI | GWGCWC | 4 cut(s) | 67, 182, 1460, 1498 |
| BsiSI | CCGG | 3 cut(s) | 250, 1318, 1464 |
| BslFI | GGGAC | 3 cut(s) | 358, 884, 1027 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 162, 278, 917, 1274, 1415, 1467 |
| BsmAI | GTCTC | 4 cut(s) | 138, 850, 977, 1569 |
| BsmFI | GGGAC | 3 cut(s) | 358, 884, 1027 |
| BsnI | GGCC | 3 cut(s) | 155, 169, 880 |
| Bso31I | GGTCTC | 1 cut(s) | 138 |
| Bsp119I | TTCGAA | 1 cut(s) | 557 |
| Bsp1286I | GDGCHC | 5 cut(s) | 67, 182, 685, 1460, 1498 |
| Bsp1407I | TGTACA | 1 cut(s) | 1044 |
| Bsp143I | GATC | 7 cut(s) | 11, 388, 1106, 1144, 1207, 1231, 1375 |
| Bsp19I | CCATGG | 1 cut(s) | 183 |
| BspACI | CCGC | 5 cut(s) | 5, 100, 170, 435, 803 |
| BspANI | GGCC | 3 cut(s) | 155, 169, 880 |
| BspFNI | CGCG | 3 cut(s) | 69, 71, 437 |
| BspHI | TCATGA | 1 cut(s) | 1305 |
| BspLI | GGNNCC | 3 cut(s) | 402, 872, 879 |
| BspMI | ACCTGC | 1 cut(s) | 207 |
| BspPI | GGATC | 2 cut(s) | 1114, 1202 |
| BspQI | GCTCTTC | 1 cut(s) | 1486 |
| BspT104I | TTCGAA | 1 cut(s) | 557 |
| BspTI | CTTAAG | 1 cut(s) | 977 |
| BspTNI | GGTCTC | 1 cut(s) | 138 |
| BsrFI | RCCGGY | 1 cut(s) | 249 |
| BsrGI | TGTACA | 1 cut(s) | 1044 |
| BsrI | ACTGG | 4 cut(s) | 259, 385, 1005, 1288 |
| BssAI | RCCGGY | 1 cut(s) | 249 |
| BssECI | CCNNGG | 5 cut(s) | 150, 183, 874, 1112, 1462 |
| BssHII | GCGCGC | 1 cut(s) | 69 |
| BssMI | GATC | 7 cut(s) | 11, 388, 1106, 1144, 1207, 1231, 1375 |
| BssNI | GRCGYC | 1 cut(s) | 347 |
| BssSI | CACGAG | 1 cut(s) | 470 |
| BssT1I | CCWWGG | 3 cut(s) | 150, 183, 1112 |
| Bst2BI | CACGAG | 1 cut(s) | 470 |
| Bst2UI | CCWGG | 2 cut(s) | 728, 875 |
| Bst4CI | ACNGT | 7 cut(s) | 292, 848, 1010, 1193, 1229, 1291, 1324 |
| Bst6I | CTCTTC | 2 cut(s) | 272, 1486 |
| BstACI | GRCGYC | 1 cut(s) | 347 |
| BstAFI | CTTAAG | 1 cut(s) | 977 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 420, 1091 |
| BstAUI | TGTACA | 1 cut(s) | 1044 |
| BstBAI | YACGTR | 1 cut(s) | 330 |
| BstBI | TTCGAA | 1 cut(s) | 557 |
| BstC8I | GCNNGC | 8 cut(s) | 67, 71, 204, 251, 503, 882, 955, 1269 |
| BstDEI | CTNAG | 3 cut(s) | 157, 640, 1574 |
| BstDSI | CCRYGG | 1 cut(s) | 183 |
| BstEII | GGTNACC | 2 cut(s) | 722, 1313 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1413 |
| BstF5I | GGATG | 4 cut(s) | 704, 736, 899, 1535 |
| BstFNI | CGCG | 3 cut(s) | 69, 71, 437 |
| BstHHI | GCGC | 4 cut(s) | 71, 73, 425, 1421 |
| BstKTI | GATC | 7 cut(s) | 14, 391, 1109, 1147, 1210, 1234, 1378 |
| BstMAI | GTCTC | 4 cut(s) | 138, 850, 977, 1569 |
| BstMBI | GATC | 7 cut(s) | 11, 388, 1106, 1144, 1207, 1231, 1375 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 52, 420, 668, 1091, 1100, 1156, 1493 |
| BstNI | CCWGG | 2 cut(s) | 728, 875 |
| BstNSI | RCATGY | 3 cut(s) | 509, 570, 957 |
| BstPI | GGTNACC | 2 cut(s) | 722, 1313 |
| BstSCI | CCNGG | 3 cut(s) | 726, 873, 1462 |
| BstUI | CGCG | 3 cut(s) | 69, 71, 437 |
| BstV1I | GCAGC | 4 cut(s) | 528, 656, 1462, 1501 |
| BstV2I | GAAGAC | 1 cut(s) | 125 |
| BstX2I | RGATCY | 3 cut(s) | 1106, 1144, 1375 |
| BstYI | RGATCY | 3 cut(s) | 1106, 1144, 1375 |
| Bsu36I | CCTNAGG | 1 cut(s) | 157 |
| BsuI | GTATCC | 1 cut(s) | 370 |
| BsuRI | GGCC | 3 cut(s) | 155, 169, 880 |
| BtgI | CCRYGG | 1 cut(s) | 183 |
| BtsCI | GGATG | 4 cut(s) | 704, 736, 899, 1535 |
| BtsIMutI | CAGTG | 3 cut(s) | 998, 1015, 1047 |
| BveI | ACCTGC | 1 cut(s) | 207 |
| Cac8I | GCNNGC | 8 cut(s) | 67, 71, 204, 251, 503, 882, 955, 1269 |
| CciI | TCATGA | 1 cut(s) | 1305 |
| CfoI | GCGC | 4 cut(s) | 71, 73, 425, 1421 |
| Cfr10I | RCCGGY | 1 cut(s) | 249 |
| Cfr13I | GGNCC | 4 cut(s) | 539, 871, 878, 1201 |
| CseI | GACGC | 3 cut(s) | 130, 355, 1177 |
| Csp6I | GTAC | 4 cut(s) | 475, 586, 1045, 1120 |
| CviAII | CATG | 8 cut(s) | 184, 405, 506, 521, 567, 954, 1205, 1306 |
| CviQI | GTAC | 4 cut(s) | 475, 586, 1045, 1120 |
| DdeI | CTNAG | 3 cut(s) | 157, 640, 1574 |
| DpnI | GATC | 7 cut(s) | 13, 390, 1108, 1146, 1209, 1233, 1377 |
| DpnII | GATC | 7 cut(s) | 11, 388, 1106, 1144, 1207, 1231, 1375 |
| EaeI | YGGCCR | 1 cut(s) | 167 |
| Eam1104I | CTCTTC | 2 cut(s) | 272, 1486 |
| EarI | CTCTTC | 2 cut(s) | 272, 1486 |
| Ecl136II | GAGCTC | 1 cut(s) | 1496 |
| Eco130I | CCWWGG | 3 cut(s) | 150, 183, 1112 |
| Eco147I | AGGCCT | 1 cut(s) | 155 |
| Eco24I | GRGCYC | 2 cut(s) | 685, 1498 |
| Eco31I | GGTCTC | 1 cut(s) | 138 |
| Eco47I | GGWCC | 3 cut(s) | 539, 871, 1201 |
| Eco53kI | GAGCTC | 1 cut(s) | 1496 |
| Eco57I | CTGAAG | 2 cut(s) | 297, 762 |
| Eco81I | CCTNAGG | 1 cut(s) | 157 |
| Eco91I | GGTNACC | 2 cut(s) | 722, 1313 |
| EcoICRI | GAGCTC | 1 cut(s) | 1496 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1413 |
| EcoO65I | GGTNACC | 2 cut(s) | 722, 1313 |
| EcoRI | GAATTC | 1 cut(s) | 553 |
| EcoRII | CCWGG | 2 cut(s) | 726, 873 |
| EcoT14I | CCWWGG | 3 cut(s) | 150, 183, 1112 |
| EcoT22I | ATGCAT | 1 cut(s) | 664 |
| EcoT38I | GRGCYC | 2 cut(s) | 685, 1498 |
| ErhI | CCWWGG | 3 cut(s) | 150, 183, 1112 |
| FaeI | CATG | 8 cut(s) | 187, 408, 509, 524, 570, 957, 1208, 1309 |
| FaqI | GGGAC | 3 cut(s) | 358, 884, 1027 |
| FatI | CATG | 8 cut(s) | 183, 404, 505, 520, 566, 953, 1204, 1305 |
| FauI | CCCGC | 1 cut(s) | 796 |
| FbaI | TGATCA | 1 cut(s) | 1231 |
| Fnu4HI | GCNGC | 6 cut(s) | 6, 170, 517, 645, 1451, 1515 |
| FokI | GGATG | 4 cut(s) | 711, 743, 886, 1522 |
| FriOI | GRGCYC | 2 cut(s) | 685, 1498 |
| Fsp4HI | GCNGC | 6 cut(s) | 6, 170, 517, 645, 1451, 1515 |
| FspBI | CTAG | 4 cut(s) | 32, 1349, 1403, 1603 |
| GlaI | GCGC | 4 cut(s) | 70, 72, 424, 1420 |
| GluI | GCNGC | 6 cut(s) | 6, 170, 517, 645, 1451, 1515 |
| HaeIII | GGCC | 3 cut(s) | 155, 169, 880 |
| HapII | CCGG | 3 cut(s) | 250, 1318, 1464 |
| HgaI | GACGC | 3 cut(s) | 130, 355, 1177 |
| HhaI | GCGC | 4 cut(s) | 71, 73, 425, 1421 |
| Hin1I | GRCGYC | 1 cut(s) | 347 |
| Hin1II | CATG | 8 cut(s) | 187, 408, 509, 524, 570, 957, 1208, 1309 |
| Hin6I | GCGC | 4 cut(s) | 69, 71, 423, 1419 |
| HinP1I | GCGC | 4 cut(s) | 69, 71, 423, 1419 |
| HincII | GTYRAC | 1 cut(s) | 1006 |
| HindII | GTYRAC | 1 cut(s) | 1006 |
| HindIII | AAGCTT | 1 cut(s) | 988 |
| HinfI | GANTC | 5 cut(s) | 533, 1037, 1248, 1522, 1543 |
| HpaII | CCGG | 3 cut(s) | 250, 1318, 1464 |
| HphI | GGTGA | 2 cut(s) | 665, 1241 |
| Hpy166II | GTNNAC | 2 cut(s) | 1006, 1045 |
| Hpy188I | TCNGA | 4 cut(s) | 109, 129, 277, 1221 |
| Hpy188III | TCNNGA | 8 cut(s) | 220, 455, 612, 743, 826, 1034, 1142, 1306 |
| Hpy8I | GTNNAC | 2 cut(s) | 1006, 1045 |
| Hpy99I | CGWCG | 2 cut(s) | 88, 113 |
| HpyAV | CCTTC | 5 cut(s) | 272, 913, 921, 1170, 1557 |
| HpyCH4III | ACNGT | 7 cut(s) | 292, 848, 1010, 1193, 1229, 1291, 1324 |
| HpyCH4IV | ACGT | 1 cut(s) | 329 |
| HpyCH4V | TGCA | 9 cut(s) | 371, 414, 516, 549, 662, 1058, 1085, 1103, 1517 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 52, 420, 668, 1091, 1100, 1156, 1493 |
| HpyF3I | CTNAG | 3 cut(s) | 157, 640, 1574 |
| HpySE526I | ACGT | 1 cut(s) | 329 |
| Hsp92I | GRCGYC | 1 cut(s) | 347 |
| Hsp92II | CATG | 8 cut(s) | 187, 408, 509, 524, 570, 957, 1208, 1309 |
| HspAI | GCGC | 4 cut(s) | 69, 71, 423, 1419 |
| KroI | GCCGGC | 1 cut(s) | 249 |
| KroNI | GCCGGC | 1 cut(s) | 251 |
| Ksp22I | TGATCA | 1 cut(s) | 1231 |
| Kzo9I | GATC | 7 cut(s) | 11, 388, 1106, 1144, 1207, 1231, 1375 |
| LguI | GCTCTTC | 1 cut(s) | 1486 |
| LmnI | GCTCC | 5 cut(s) | 177, 430, 745, 1480, 1501 |
| Lsp1109I | GCAGC | 4 cut(s) | 528, 656, 1462, 1501 |
| LweI | GCATC | 3 cut(s) | 649, 671, 1526 |
| MaeI | CTAG | 4 cut(s) | 32, 1349, 1403, 1603 |
| MaeII | ACGT | 1 cut(s) | 329 |
| MaeIII | GTNAC | 4 cut(s) | 318, 722, 1165, 1313 |
| MalI | GATC | 7 cut(s) | 13, 390, 1108, 1146, 1209, 1233, 1377 |
| MboI | GATC | 7 cut(s) | 11, 388, 1106, 1144, 1207, 1231, 1375 |
| MboII | GAAGA | 5 cut(s) | 130, 259, 1022, 1503, 1585 |
| MfeI | CAATTG | 1 cut(s) | 1089 |
| MflI | RGATCY | 3 cut(s) | 1106, 1144, 1375 |
| MhlI | GDGCHC | 5 cut(s) | 67, 182, 685, 1460, 1498 |
| MluCI | AATT | 7 cut(s) | 553, 735, 922, 967, 1053, 1089, 1366 |
| MlyI | GAGTC | 2 cut(s) | 1046, 1531 |
| MmeI | TCCRAC | 2 cut(s) | 132, 1273 |
| Mph1103I | ATGCAT | 1 cut(s) | 664 |
| MroNI | GCCGGC | 1 cut(s) | 249 |
| MroXI | GAANNNNTTC | 1 cut(s) | 821 |
| MseI | TTAA | 3 cut(s) | 978, 1242, 1389 |
| MspA1I | CMGCKG | 2 cut(s) | 647, 1514 |
| MspCI | CTTAAG | 1 cut(s) | 977 |
| MspI | CCGG | 3 cut(s) | 250, 1318, 1464 |
| MspR9I | CCNGG | 3 cut(s) | 728, 875, 1464 |
| MunI | CAATTG | 1 cut(s) | 1089 |
| MvaI | CCWGG | 2 cut(s) | 728, 875 |
| MvnI | CGCG | 3 cut(s) | 69, 71, 437 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 52, 420, 668, 1091, 1100, 1156, 1493 |
| NaeI | GCCGGC | 1 cut(s) | 251 |
| NciI | CCSGG | 1 cut(s) | 1464 |
| NcoI | CCATGG | 1 cut(s) | 183 |
| NdeII | GATC | 7 cut(s) | 11, 388, 1106, 1144, 1207, 1231, 1375 |
| NgoMIV | GCCGGC | 1 cut(s) | 249 |
| NlaIII | CATG | 8 cut(s) | 187, 408, 509, 524, 570, 957, 1208, 1309 |
| NlaIV | GGNNCC | 3 cut(s) | 402, 872, 879 |
| NmeAIII | GCCGAG | 2 cut(s) | 219, 1397 |
| NmuCI | GTSAC | 1 cut(s) | 1165 |
| NsiI | ATGCAT | 1 cut(s) | 664 |
| NspI | RCATGY | 3 cut(s) | 509, 570, 957 |
| NspV | TTCGAA | 1 cut(s) | 557 |
| PaeI | GCATGC | 1 cut(s) | 957 |
| PagI | TCATGA | 1 cut(s) | 1305 |
| PaqCI | CACCTGC | 1 cut(s) | 207 |
| PauI | GCGCGC | 1 cut(s) | 69 |
| PceI | AGGCCT | 1 cut(s) | 155 |
| PciSI | GCTCTTC | 1 cut(s) | 1486 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 83, 210 |
| PdiI | GCCGGC | 1 cut(s) | 251 |
| PdmI | GAANNNNTTC | 1 cut(s) | 821 |
| PfeI | GAWTC | 3 cut(s) | 533, 1248, 1543 |
| PkrI | GCNGC | 6 cut(s) | 7, 171, 518, 646, 1452, 1516 |
| PleI | GAGTC | 2 cut(s) | 1045, 1530 |
| PpsI | GAGTC | 2 cut(s) | 1045, 1530 |
| Ppu21I | YACGTR | 1 cut(s) | 330 |
| Psp124BI | GAGCTC | 1 cut(s) | 1498 |
| Psp6I | CCWGG | 2 cut(s) | 726, 873 |
| PspEI | GGTNACC | 2 cut(s) | 722, 1313 |
| PspGI | CCWGG | 2 cut(s) | 726, 873 |
| PspN4I | GGNNCC | 3 cut(s) | 402, 872, 879 |
| PspPI | GGNCC | 4 cut(s) | 539, 871, 878, 1201 |
| PsuI | RGATCY | 3 cut(s) | 1106, 1144, 1375 |
| PteI | GCGCGC | 1 cut(s) | 69 |
| PvuII | CAGCTG | 2 cut(s) | 647, 1514 |
| RsaI | GTAC | 4 cut(s) | 476, 587, 1046, 1121 |
| RsaNI | GTAC | 4 cut(s) | 475, 586, 1045, 1120 |
| SacI | GAGCTC | 1 cut(s) | 1498 |
| SapI | GCTCTTC | 1 cut(s) | 1486 |
| SaqAI | TTAA | 3 cut(s) | 978, 1242, 1389 |
| SatI | GCNGC | 6 cut(s) | 6, 170, 517, 645, 1451, 1515 |
| Sau3AI | GATC | 7 cut(s) | 11, 388, 1106, 1144, 1207, 1231, 1375 |
| Sau96I | GGNCC | 4 cut(s) | 539, 871, 878, 1201 |
| ScaI | AGTACT | 2 cut(s) | 587, 1121 |
| SchI | GAGTC | 2 cut(s) | 1046, 1531 |
| ScrFI | CCNGG | 3 cut(s) | 728, 875, 1464 |
| SduI | GDGCHC | 5 cut(s) | 67, 182, 685, 1460, 1498 |
| SfaNI | GCATC | 3 cut(s) | 649, 671, 1526 |
| SfuI | TTCGAA | 1 cut(s) | 557 |
| SinI | GGWCC | 3 cut(s) | 539, 871, 1201 |
| SmlI | CTYRAG | 1 cut(s) | 977 |
| SmoI | CTYRAG | 1 cut(s) | 977 |
| SphI | GCATGC | 1 cut(s) | 957 |
| Sse9I | AATT | 7 cut(s) | 553, 735, 922, 967, 1053, 1089, 1366 |
| SseBI | AGGCCT | 1 cut(s) | 155 |
| SsiI | CCGC | 5 cut(s) | 5, 100, 170, 435, 803 |
| SspMI | CTAG | 4 cut(s) | 32, 1349, 1403, 1603 |
| SstI | GAGCTC | 1 cut(s) | 1498 |
| StuI | AGGCCT | 1 cut(s) | 155 |
| StyD4I | CCNGG | 3 cut(s) | 726, 873, 1462 |
| StyI | CCWWGG | 3 cut(s) | 150, 183, 1112 |
| TaaI | ACNGT | 7 cut(s) | 292, 848, 1010, 1193, 1229, 1291, 1324 |
| TaiI | ACGT | 1 cut(s) | 332 |
| TaqI | TCGA | 9 cut(s) | 10, 86, 134, 213, 557, 679, 827, 1329, 1520 |
| TasI | AATT | 7 cut(s) | 553, 735, 922, 967, 1053, 1089, 1366 |
| TatI | WGTACW | 4 cut(s) | 474, 585, 1044, 1119 |
| TauI | GCSGC | 2 cut(s) | 8, 172 |
| TfiI | GAWTC | 3 cut(s) | 533, 1248, 1543 |
| Tru1I | TTAA | 3 cut(s) | 978, 1242, 1389 |
| Tru9I | TTAA | 3 cut(s) | 978, 1242, 1389 |
| TscAI | CASTG | 3 cut(s) | 1005, 1015, 1047 |
| TseFI | GTSAC | 1 cut(s) | 1165 |
| TseI | GCWGC | 4 cut(s) | 516, 644, 1450, 1514 |
| Tsp45I | GTSAC | 1 cut(s) | 1165 |
| TspDTI | ATGAA | 5 cut(s) | 47, 248, 722, 942, 1294 |
| TspRI | CASTG | 3 cut(s) | 1005, 1015, 1047 |
| Vha464I | CTTAAG | 1 cut(s) | 977 |
| VpaK11BI | GGWCC | 3 cut(s) | 539, 871, 1201 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1413 |
| XapI | RAATTY | 2 cut(s) | 553, 967 |
| XceI | RCATGY | 3 cut(s) | 509, 570, 957 |
| XmnI | GAANNNNTTC | 1 cut(s) | 821 |
| XspI | CTAG | 4 cut(s) | 32, 1349, 1403, 1603 |
| ZrmI | AGTACT | 2 cut(s) | 587, 1121 |
| Zsp2I | ATGCAT | 1 cut(s) | 664 |