Belongs to the peptidase A1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 56 |
| AciI | CCGC | 1 cut(s) | 125 |
| AclWI | GGATC | 2 cut(s) | 135, 151 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 77 |
| AjnI | CCWGG | 1 cut(s) | 33 |
| AlwI | GGATC | 2 cut(s) | 135, 151 |
| ArsI | GACNNNNNNTTYG | 1 cut(s) | 186 |
| AspS9I | GGNCC | 2 cut(s) | 31, 38 |
| AvaII | GGWCC | 2 cut(s) | 31, 38 |
| BciT130I | CCWGG | 1 cut(s) | 35 |
| Bme1390I | CCNGG | 1 cut(s) | 35 |
| Bme18I | GGWCC | 2 cut(s) | 31, 38 |
| BmgT120I | GGNCC | 2 cut(s) | 31, 38 |
| BmiI | GGNNCC | 2 cut(s) | 32, 39 |
| BmrFI | CCNGG | 1 cut(s) | 35 |
| BsaJI | CCNNGG | 1 cut(s) | 34 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 77 |
| BseBI | CCWGG | 1 cut(s) | 35 |
| BseDI | CCNNGG | 1 cut(s) | 34 |
| BseGI | GGATG | 1 cut(s) | 59 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 77 |
| BslFI | GGGAC | 2 cut(s) | 44, 51 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 77 |
| BsmFI | GGGAC | 2 cut(s) | 44, 51 |
| Bsp143I | GATC | 2 cut(s) | 127, 143 |
| BspACI | CCGC | 1 cut(s) | 125 |
| BspLI | GGNNCC | 2 cut(s) | 32, 39 |
| BspPI | GGATC | 2 cut(s) | 135, 151 |
| BssECI | CCNNGG | 1 cut(s) | 34 |
| BssMI | GATC | 2 cut(s) | 127, 143 |
| Bst2UI | CCWGG | 1 cut(s) | 35 |
| Bst4CI | ACNGT | 1 cut(s) | 54 |
| BstF5I | GGATG | 1 cut(s) | 59 |
| BstKTI | GATC | 2 cut(s) | 130, 146 |
| BstMBI | GATC | 2 cut(s) | 127, 143 |
| BstNI | CCWGG | 1 cut(s) | 35 |
| BstSCI | CCNGG | 1 cut(s) | 33 |
| BtsCI | GGATG | 1 cut(s) | 59 |
| Cfr13I | GGNCC | 2 cut(s) | 31, 38 |
| CviAII | CATG | 4 cut(s) | 131, 140, 147, 156 |
| DpnI | GATC | 2 cut(s) | 129, 145 |
| DpnII | GATC | 2 cut(s) | 127, 143 |
| Eco47I | GGWCC | 2 cut(s) | 31, 38 |
| EcoRII | CCWGG | 1 cut(s) | 33 |
| FaeI | CATG | 4 cut(s) | 134, 143, 150, 159 |
| FaiI | YATR | 6 cut(s) | 87, 115, 132, 141, 148, 157 |
| FaqI | GGGAC | 2 cut(s) | 44, 51 |
| FatI | CATG | 4 cut(s) | 130, 139, 146, 155 |
| FblI | GTMKAC | 1 cut(s) | 56 |
| FokI | GGATG | 1 cut(s) | 46 |
| Hin1II | CATG | 4 cut(s) | 134, 143, 150, 159 |
| HinfI | GANTC | 1 cut(s) | 176 |
| Hpy166II | GTNNAC | 3 cut(s) | 57, 137, 153 |
| Hpy188III | TCNNGA | 1 cut(s) | 14 |
| Hpy8I | GTNNAC | 3 cut(s) | 57, 137, 153 |
| Hpy99I | CGWCG | 1 cut(s) | 54 |
| HpyAV | CCTTC | 1 cut(s) | 73 |
| HpyCH4III | ACNGT | 1 cut(s) | 54 |
| Hsp92II | CATG | 4 cut(s) | 134, 143, 150, 159 |
| Kzo9I | GATC | 2 cut(s) | 127, 143 |
| LpnPI | CCDG | 2 cut(s) | 20, 47 |
| MalI | GATC | 2 cut(s) | 129, 145 |
| MboI | GATC | 2 cut(s) | 127, 143 |
| MnlI | CCTC | 1 cut(s) | 82 |
| MspR9I | CCNGG | 1 cut(s) | 35 |
| MvaI | CCWGG | 1 cut(s) | 35 |
| NdeII | GATC | 2 cut(s) | 127, 143 |
| NlaIII | CATG | 4 cut(s) | 134, 143, 150, 159 |
| NlaIV | GGNNCC | 2 cut(s) | 32, 39 |
| PfeI | GAWTC | 1 cut(s) | 176 |
| Psp6I | CCWGG | 1 cut(s) | 33 |
| PspGI | CCWGG | 1 cut(s) | 33 |
| PspN4I | GGNNCC | 2 cut(s) | 32, 39 |
| PspPI | GGNCC | 2 cut(s) | 31, 38 |
| Sau3AI | GATC | 2 cut(s) | 127, 143 |
| Sau96I | GGNCC | 2 cut(s) | 31, 38 |
| ScrFI | CCNGG | 1 cut(s) | 35 |
| SetI | ASST | 2 cut(s) | 12, 95 |
| SinI | GGWCC | 2 cut(s) | 31, 38 |
| SsiI | CCGC | 1 cut(s) | 125 |
| SspI | AATATT | 1 cut(s) | 22 |
| StyD4I | CCNGG | 1 cut(s) | 33 |
| TaaI | ACNGT | 1 cut(s) | 54 |
| TaqI | TCGA | 1 cut(s) | 49 |
| TfiI | GAWTC | 1 cut(s) | 176 |
| TspDTI | ATGAA | 1 cut(s) | 102 |
| TspGWI | ACGGA | 1 cut(s) | 93 |
| VpaK11BI | GGWCC | 2 cut(s) | 31, 38 |
| XmiI | GTMKAC | 1 cut(s) | 56 |