Belongs to the peptidase A1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 726 |
| AccB1I | GGYRCC | 2 cut(s) | 242, 726 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1268 |
| AccII | CGCG | 2 cut(s) | 68, 172 |
| AclWI | GGATC | 1 cut(s) | 1108 |
| AcoI | YGGCCR | 3 cut(s) | 63, 167, 1523 |
| AcsI | RAATTY | 2 cut(s) | 961, 1242 |
| AcuI | CTGAAG | 1 cut(s) | 894 |
| AcyI | GRCGYC | 3 cut(s) | 147, 296, 350 |
| AfaI | GTAC | 5 cut(s) | 454, 535, 728, 1115, 1581 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 61, 281, 1268 |
| AflII | CTTAAG | 1 cut(s) | 971 |
| AgsI | TTSAA | 5 cut(s) | 472, 587, 907, 988, 1587 |
| AjnI | CCWGG | 3 cut(s) | 729, 1173, 1459 |
| AluBI | AGCT | 7 cut(s) | 440, 539, 878, 984, 1342, 1493, 1511 |
| AluI | AGCT | 7 cut(s) | 440, 539, 878, 984, 1342, 1493, 1511 |
| Alw21I | GWGCWC | 2 cut(s) | 1457, 1495 |
| Alw26I | GTCTC | 1 cut(s) | 1356 |
| AlwI | GGATC | 1 cut(s) | 1108 |
| Ama87I | CYCGRG | 1 cut(s) | 1534 |
| AoxI | GGCC | 7 cut(s) | 63, 111, 126, 167, 556, 684, 1523 |
| ApaI | GGGCCC | 1 cut(s) | 560 |
| ApeKI | GCWGC | 6 cut(s) | 135, 285, 519, 565, 1447, 1511 |
| ApoI | RAATTY | 2 cut(s) | 961, 1242 |
| AseI | ATTAAT | 1 cut(s) | 1590 |
| Asp718I | GGTACC | 1 cut(s) | 726 |
| AspLEI | GCGC | 2 cut(s) | 225, 428 |
| AspS9I | GGNCC | 5 cut(s) | 542, 556, 557, 685, 1195 |
| AsuHPI | GGTGA | 3 cut(s) | 188, 1283, 1388 |
| AvaI | CYCGRG | 1 cut(s) | 1534 |
| AvaII | GGWCC | 2 cut(s) | 542, 1195 |
| BaeGI | GKGCMC | 2 cut(s) | 560, 1418 |
| BanI | GGYRCC | 2 cut(s) | 242, 726 |
| BanII | GRGCYC | 2 cut(s) | 560, 1495 |
| BauI | CACGAG | 1 cut(s) | 1087 |
| Bbv12I | GWGCWC | 2 cut(s) | 1457, 1495 |
| BbvI | GCAGC | 6 cut(s) | 147, 297, 531, 577, 1459, 1498 |
| BccI | CCATC | 3 cut(s) | 728, 743, 1262 |
| BceAI | ACGGC | 1 cut(s) | 309 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 295, 329 |
| BciT130I | CCWGG | 3 cut(s) | 731, 1175, 1461 |
| BciVI | GTATCC | 1 cut(s) | 373 |
| BclI | TGATCA | 2 cut(s) | 693, 813 |
| BcoDI | GTCTC | 1 cut(s) | 1356 |
| BfaI | CTAG | 4 cut(s) | 770, 789, 1343, 1518 |
| BfmI | CTRYAG | 1 cut(s) | 448 |
| BfoI | RGCGCY | 1 cut(s) | 226 |
| BfrI | CTTAAG | 1 cut(s) | 971 |
| BfuI | GTATCC | 1 cut(s) | 373 |
| BglII | AGATCT | 1 cut(s) | 1369 |
| BmcAI | AGTACT | 2 cut(s) | 454, 1115 |
| Bme1390I | CCNGG | 3 cut(s) | 731, 1175, 1461 |
| Bme18I | GGWCC | 2 cut(s) | 542, 1195 |
| BmeT110I | CYCGRG | 1 cut(s) | 1534 |
| BmgT120I | GGNCC | 5 cut(s) | 542, 556, 557, 685, 1195 |
| BmiI | GGNNCC | 6 cut(s) | 244, 405, 558, 559, 687, 728 |
| BmrFI | CCNGG | 3 cut(s) | 731, 1175, 1461 |
| BmrI | ACTGGG | 1 cut(s) | 1014 |
| BmsI | GCATC | 3 cut(s) | 1369, 1514, 1523 |
| BmuI | ACTGGG | 1 cut(s) | 1014 |
| BpuEI | CTTGAG | 2 cut(s) | 107, 1119 |
| Bsa29I | ATCGAT | 1 cut(s) | 13 |
| BsaAI | YACGTR | 1 cut(s) | 333 |
| BsaHI | GRCGYC | 3 cut(s) | 147, 296, 350 |
| BsaI | GGTCTC | 1 cut(s) | 1356 |
| BsaJI | CCNNGG | 6 cut(s) | 114, 170, 183, 238, 1106, 1526 |
| BsaWI | WCCGGW | 1 cut(s) | 1304 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 30, 60, 86, 116, 1390, 1420 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 61, 281, 1268 |
| Bse118I | RCCGGY | 1 cut(s) | 140 |
| Bse1I | ACTGG | 4 cut(s) | 262, 388, 1009, 1128 |
| Bse3DI | GCAATG | 3 cut(s) | 313, 738, 1430 |
| BseBI | CCWGG | 3 cut(s) | 731, 1175, 1461 |
| BseCI | ATCGAT | 1 cut(s) | 13 |
| BseDI | CCNNGG | 6 cut(s) | 114, 170, 183, 238, 1106, 1526 |
| BseGI | GGATG | 3 cut(s) | 707, 739, 1273 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 61, 281, 1268 |
| BseMI | GCAATG | 3 cut(s) | 313, 738, 1430 |
| BseMII | CTCAG | 3 cut(s) | 609, 857, 1434 |
| BseNI | ACTGG | 4 cut(s) | 262, 388, 1009, 1128 |
| BseRI | GAGGAG | 4 cut(s) | 38, 41, 70, 191 |
| BseSI | GKGCMC | 2 cut(s) | 560, 1418 |
| BseX3I | CGGCCG | 1 cut(s) | 63 |
| BseXI | GCAGC | 6 cut(s) | 147, 297, 531, 577, 1459, 1498 |
| Bsh1236I | CGCG | 2 cut(s) | 68, 172 |
| Bsh1285I | CGRYCG | 2 cut(s) | 66, 163 |
| BshFI | GGCC | 7 cut(s) | 65, 113, 128, 169, 558, 686, 1525 |
| BshNI | GGYRCC | 2 cut(s) | 242, 726 |
| BshVI | ATCGAT | 1 cut(s) | 13 |
| BsiEI | CGRYCG | 2 cut(s) | 66, 163 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1457, 1495 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1534 |
| BsiSI | CCGG | 2 cut(s) | 141, 1305 |
| BslFI | GGGAC | 3 cut(s) | 361, 1021, 1544 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 61, 281, 1268 |
| BsmAI | GTCTC | 1 cut(s) | 1356 |
| BsmFI | GGGAC | 3 cut(s) | 361, 1021, 1544 |
| BsmI | GAATGC | 1 cut(s) | 1081 |
| BsnI | GGCC | 7 cut(s) | 65, 113, 128, 169, 558, 686, 1525 |
| Bso31I | GGTCTC | 1 cut(s) | 1356 |
| BsoBI | CYCGRG | 1 cut(s) | 1534 |
| Bsp120I | GGGCCC | 1 cut(s) | 556 |
| Bsp1286I | GDGCHC | 4 cut(s) | 560, 1418, 1457, 1495 |
| Bsp1407I | TGTACA | 1 cut(s) | 533 |
| Bsp143I | GATC | 6 cut(s) | 14, 391, 693, 813, 1100, 1369 |
| Bsp19I | CCATGG | 2 cut(s) | 183, 1106 |
| BspANI | GGCC | 7 cut(s) | 65, 113, 128, 169, 558, 686, 1525 |
| BspCNI | CTCAG | 3 cut(s) | 608, 858, 1435 |
| BspDI | ATCGAT | 1 cut(s) | 13 |
| BspFNI | CGCG | 2 cut(s) | 68, 172 |
| BspHI | TCATGA | 1 cut(s) | 1299 |
| BspLI | GGNNCC | 6 cut(s) | 244, 405, 558, 559, 687, 728 |
| BspPI | GGATC | 1 cut(s) | 1108 |
| BspQI | GCTCTTC | 1 cut(s) | 1483 |
| BspT107I | GGYRCC | 2 cut(s) | 242, 726 |
| BspTI | CTTAAG | 1 cut(s) | 971 |
| BspTNI | GGTCTC | 1 cut(s) | 1356 |
| BsrDI | GCAATG | 3 cut(s) | 313, 738, 1430 |
| BsrFI | RCCGGY | 1 cut(s) | 140 |
| BsrGI | TGTACA | 1 cut(s) | 533 |
| BsrI | ACTGG | 4 cut(s) | 262, 388, 1009, 1128 |
| BssAI | RCCGGY | 1 cut(s) | 140 |
| BssECI | CCNNGG | 6 cut(s) | 114, 170, 183, 238, 1106, 1526 |
| BssMI | GATC | 6 cut(s) | 14, 391, 693, 813, 1100, 1369 |
| BssNI | GRCGYC | 3 cut(s) | 147, 296, 350 |
| BssSI | CACGAG | 1 cut(s) | 1087 |
| BssT1I | CCWWGG | 4 cut(s) | 114, 183, 1106, 1526 |
| Bst2BI | CACGAG | 1 cut(s) | 1087 |
| Bst2UI | CCWGG | 3 cut(s) | 731, 1175, 1461 |
| Bst4CI | ACNGT | 4 cut(s) | 662, 903, 1187, 1318 |
| Bst6I | CTCTTC | 2 cut(s) | 275, 1483 |
| BstACI | GRCGYC | 3 cut(s) | 147, 296, 350 |
| BstAFI | CTTAAG | 1 cut(s) | 971 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1511 |
| BstAUI | TGTACA | 1 cut(s) | 533 |
| BstBAI | YACGTR | 1 cut(s) | 333 |
| BstC8I | GCNNGC | 3 cut(s) | 208, 506, 1263 |
| BstDEI | CTNAG | 5 cut(s) | 595, 866, 879, 1383, 1443 |
| BstDSI | CCRYGG | 3 cut(s) | 170, 183, 1106 |
| BstF5I | GGATG | 3 cut(s) | 707, 739, 1273 |
| BstFNI | CGCG | 2 cut(s) | 68, 172 |
| BstH2I | RGCGCY | 1 cut(s) | 226 |
| BstHHI | GCGC | 2 cut(s) | 225, 428 |
| BstKTI | GATC | 6 cut(s) | 17, 394, 696, 816, 1103, 1372 |
| BstMAI | GTCTC | 1 cut(s) | 1356 |
| BstMBI | GATC | 6 cut(s) | 14, 391, 693, 813, 1100, 1369 |
| BstMCI | CGRYCG | 2 cut(s) | 66, 163 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 125, 141, 423, 1150, 1353, 1490, 1511 |
| BstNI | CCWGG | 3 cut(s) | 731, 1175, 1461 |
| BstNSI | RCATGY | 2 cut(s) | 512, 951 |
| BstSCI | CCNGG | 3 cut(s) | 729, 1173, 1459 |
| BstSFI | CTRYAG | 1 cut(s) | 448 |
| BstSLI | GKGCMC | 2 cut(s) | 560, 1418 |
| BstUI | CGCG | 2 cut(s) | 68, 172 |
| BstV1I | GCAGC | 6 cut(s) | 147, 297, 531, 577, 1459, 1498 |
| BstX2I | RGATCY | 2 cut(s) | 1100, 1369 |
| BstYI | RGATCY | 2 cut(s) | 1100, 1369 |
| BstZI | CGGCCG | 1 cut(s) | 63 |
| Bsu15I | ATCGAT | 1 cut(s) | 13 |
| BsuI | GTATCC | 1 cut(s) | 373 |
| BsuRI | GGCC | 7 cut(s) | 65, 113, 128, 169, 558, 686, 1525 |
| BsuTUI | ATCGAT | 1 cut(s) | 13 |
| BtgI | CCRYGG | 3 cut(s) | 170, 183, 1106 |
| BtsCI | GGATG | 3 cut(s) | 707, 739, 1273 |
| BtsI | GCAGTG | 1 cut(s) | 1059 |
| BtsIMutI | CAGTG | 2 cut(s) | 899, 1059 |
| Cac8I | GCNNGC | 3 cut(s) | 208, 506, 1263 |
| CciI | TCATGA | 1 cut(s) | 1299 |
| CciNI | GCGGCCGC | 1 cut(s) | 63 |
| CfoI | GCGC | 2 cut(s) | 225, 428 |
| Cfr10I | RCCGGY | 1 cut(s) | 140 |
| Cfr13I | GGNCC | 5 cut(s) | 542, 556, 557, 685, 1195 |
| Cfr42I | CCGCGG | 1 cut(s) | 173 |
| ClaI | ATCGAT | 1 cut(s) | 13 |
| CseI | GACGC | 4 cut(s) | 155, 285, 358, 1171 |
| Csp6I | GTAC | 5 cut(s) | 453, 534, 727, 1114, 1580 |
| CviAII | CATG | 9 cut(s) | 184, 408, 509, 524, 948, 1107, 1199, 1300, 1554 |
| CviQI | GTAC | 5 cut(s) | 453, 534, 727, 1114, 1580 |
| DdeI | CTNAG | 5 cut(s) | 595, 866, 879, 1383, 1443 |
| DpnI | GATC | 6 cut(s) | 16, 393, 695, 815, 1102, 1371 |
| DpnII | GATC | 6 cut(s) | 14, 391, 693, 813, 1100, 1369 |
| EaeI | YGGCCR | 3 cut(s) | 63, 167, 1523 |
| EagI | CGGCCG | 1 cut(s) | 63 |
| Eam1104I | CTCTTC | 2 cut(s) | 275, 1483 |
| EarI | CTCTTC | 2 cut(s) | 275, 1483 |
| EciI | GGCGGA | 1 cut(s) | 1485 |
| Ecl136II | GAGCTC | 1 cut(s) | 1493 |
| EclXI | CGGCCG | 1 cut(s) | 63 |
| Eco130I | CCWWGG | 4 cut(s) | 114, 183, 1106, 1526 |
| Eco147I | AGGCCT | 1 cut(s) | 128 |
| Eco24I | GRGCYC | 2 cut(s) | 560, 1495 |
| Eco31I | GGTCTC | 1 cut(s) | 1356 |
| Eco32I | GATATC | 1 cut(s) | 1310 |
| Eco47I | GGWCC | 2 cut(s) | 542, 1195 |
| Eco52I | CGGCCG | 1 cut(s) | 63 |
| Eco53kI | GAGCTC | 1 cut(s) | 1493 |
| Eco57I | CTGAAG | 1 cut(s) | 894 |
| Eco88I | CYCGRG | 1 cut(s) | 1534 |
| EcoICRI | GAGCTC | 1 cut(s) | 1493 |
| EcoO109I | RGGNCCY | 3 cut(s) | 556, 557, 685 |
| EcoRII | CCWGG | 3 cut(s) | 729, 1173, 1459 |
| EcoRV | GATATC | 1 cut(s) | 1310 |
| EcoT14I | CCWWGG | 4 cut(s) | 114, 183, 1106, 1526 |
| EcoT38I | GRGCYC | 2 cut(s) | 560, 1495 |
| ErhI | CCWWGG | 4 cut(s) | 114, 183, 1106, 1526 |
| FaeI | CATG | 9 cut(s) | 187, 411, 512, 527, 951, 1110, 1202, 1303, 1557 |
| FaqI | GGGAC | 3 cut(s) | 361, 1021, 1544 |
| FatI | CATG | 9 cut(s) | 183, 407, 508, 523, 947, 1106, 1198, 1299, 1553 |
| FauI | CCCGC | 3 cut(s) | 92, 140, 643 |
| FbaI | TGATCA | 2 cut(s) | 693, 813 |
| FokI | GGATG | 3 cut(s) | 714, 746, 1280 |
| FriOI | GRGCYC | 2 cut(s) | 560, 1495 |
| FspBI | CTAG | 4 cut(s) | 770, 789, 1343, 1518 |
| GlaI | GCGC | 2 cut(s) | 224, 427 |
| HaeII | RGCGCY | 1 cut(s) | 226 |
| HaeIII | GGCC | 7 cut(s) | 65, 113, 128, 169, 558, 686, 1525 |
| HapII | CCGG | 2 cut(s) | 141, 1305 |
| HgaI | GACGC | 4 cut(s) | 155, 285, 358, 1171 |
| HhaI | GCGC | 2 cut(s) | 225, 428 |
| Hin1I | GRCGYC | 3 cut(s) | 147, 296, 350 |
| Hin1II | CATG | 9 cut(s) | 187, 411, 512, 527, 951, 1110, 1202, 1303, 1557 |
| Hin6I | GCGC | 2 cut(s) | 223, 426 |
| HinP1I | GCGC | 2 cut(s) | 223, 426 |
| HindIII | AAGCTT | 1 cut(s) | 982 |
| HinfI | GANTC | 2 cut(s) | 883, 1532 |
| HpaII | CCGG | 2 cut(s) | 141, 1305 |
| HphI | GGTGA | 3 cut(s) | 188, 1283, 1388 |
| Hpy166II | GTNNAC | 1 cut(s) | 1580 |
| Hpy188I | TCNGA | 6 cut(s) | 106, 280, 598, 867, 874, 1314 |
| Hpy188III | TCNNGA | 9 cut(s) | 458, 615, 746, 1028, 1087, 1136, 1213, 1300, 1367 |
| Hpy8I | GTNNAC | 1 cut(s) | 1580 |
| Hpy99I | CGWCG | 3 cut(s) | 110, 149, 301 |
| HpyAV | CCTTC | 6 cut(s) | 41, 275, 1034, 1091, 1164, 1413 |
| HpyCH4III | ACNGT | 4 cut(s) | 662, 903, 1187, 1318 |
| HpyCH4IV | ACGT | 1 cut(s) | 332 |
| HpyCH4V | TGCA | 5 cut(s) | 325, 519, 552, 1052, 1514 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 125, 141, 423, 1150, 1353, 1490, 1511 |
| HpyF3I | CTNAG | 5 cut(s) | 595, 866, 879, 1383, 1443 |
| HpySE526I | ACGT | 1 cut(s) | 332 |
| Hsp92I | GRCGYC | 3 cut(s) | 147, 296, 350 |
| Hsp92II | CATG | 9 cut(s) | 187, 411, 512, 527, 951, 1110, 1202, 1303, 1557 |
| HspAI | GCGC | 2 cut(s) | 223, 426 |
| KpnI | GGTACC | 1 cut(s) | 730 |
| Ksp22I | TGATCA | 2 cut(s) | 693, 813 |
| KspI | CCGCGG | 1 cut(s) | 173 |
| Kzo9I | GATC | 6 cut(s) | 14, 391, 693, 813, 1100, 1369 |
| LguI | GCTCTTC | 1 cut(s) | 1483 |
| LmnI | GCTCC | 4 cut(s) | 433, 748, 1477, 1498 |
| Lsp1109I | GCAGC | 6 cut(s) | 147, 297, 531, 577, 1459, 1498 |
| LweI | GCATC | 3 cut(s) | 1369, 1514, 1523 |
| MaeI | CTAG | 4 cut(s) | 770, 789, 1343, 1518 |
| MaeII | ACGT | 1 cut(s) | 332 |
| MaeIII | GTNAC | 2 cut(s) | 656, 1159 |
| MalI | GATC | 6 cut(s) | 16, 393, 695, 815, 1102, 1371 |
| MboI | GATC | 6 cut(s) | 14, 391, 693, 813, 1100, 1369 |
| MboII | GAAGA | 4 cut(s) | 241, 262, 1043, 1500 |
| MflI | RGATCY | 2 cut(s) | 1100, 1369 |
| MhlI | GDGCHC | 4 cut(s) | 560, 1418, 1457, 1495 |
| MluCI | AATT | 7 cut(s) | 673, 961, 1047, 1242, 1425, 1587, 1591 |
| MlyI | GAGTC | 1 cut(s) | 1526 |
| MmeI | TCCRAC | 2 cut(s) | 129, 1219 |
| MseI | TTAA | 3 cut(s) | 852, 972, 1590 |
| MspA1I | CMGCKG | 4 cut(s) | 172, 539, 1265, 1511 |
| MspCI | CTTAAG | 1 cut(s) | 971 |
| MspI | CCGG | 2 cut(s) | 141, 1305 |
| MspR9I | CCNGG | 3 cut(s) | 731, 1175, 1461 |
| Mva1269I | GAATGC | 1 cut(s) | 1081 |
| MvaI | CCWGG | 3 cut(s) | 731, 1175, 1461 |
| MvnI | CGCG | 2 cut(s) | 68, 172 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 125, 141, 423, 1150, 1353, 1490, 1511 |
| NcoI | CCATGG | 2 cut(s) | 183, 1106 |
| NdeII | GATC | 6 cut(s) | 14, 391, 693, 813, 1100, 1369 |
| NlaIII | CATG | 9 cut(s) | 187, 411, 512, 527, 951, 1110, 1202, 1303, 1557 |
| NlaIV | GGNNCC | 6 cut(s) | 244, 405, 558, 559, 687, 728 |
| NmeAIII | GCCGAG | 1 cut(s) | 219 |
| NmuCI | GTSAC | 1 cut(s) | 1159 |
| NotI | GCGGCCGC | 1 cut(s) | 63 |
| NspI | RCATGY | 2 cut(s) | 512, 951 |
| PaeR7I | CTCGAG | 1 cut(s) | 1534 |
| PagI | TCATGA | 1 cut(s) | 1299 |
| PceI | AGGCCT | 1 cut(s) | 128 |
| PciSI | GCTCTTC | 1 cut(s) | 1483 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 210 |
| PctI | GAATGC | 1 cut(s) | 1081 |
| PfeI | GAWTC | 1 cut(s) | 883 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1268 |
| PleI | GAGTC | 1 cut(s) | 1526 |
| PpsI | GAGTC | 1 cut(s) | 1526 |
| Ppu21I | YACGTR | 1 cut(s) | 333 |
| PshBI | ATTAAT | 1 cut(s) | 1590 |
| Psp124BI | GAGCTC | 1 cut(s) | 1495 |
| Psp6I | CCWGG | 3 cut(s) | 729, 1173, 1459 |
| PspGI | CCWGG | 3 cut(s) | 729, 1173, 1459 |
| PspN4I | GGNNCC | 6 cut(s) | 244, 405, 558, 559, 687, 728 |
| PspOMI | GGGCCC | 1 cut(s) | 556 |
| PspPI | GGNCC | 5 cut(s) | 542, 556, 557, 685, 1195 |
| PspXI | VCTCGAGB | 1 cut(s) | 1534 |
| PsuI | RGATCY | 2 cut(s) | 1100, 1369 |
| PvuII | CAGCTG | 2 cut(s) | 539, 1511 |
| RsaI | GTAC | 5 cut(s) | 454, 535, 728, 1115, 1581 |
| RsaNI | GTAC | 5 cut(s) | 453, 534, 727, 1114, 1580 |
| SacI | GAGCTC | 1 cut(s) | 1495 |
| SacII | CCGCGG | 1 cut(s) | 173 |
| SapI | GCTCTTC | 1 cut(s) | 1483 |
| SaqAI | TTAA | 3 cut(s) | 852, 972, 1590 |
| Sau3AI | GATC | 6 cut(s) | 14, 391, 693, 813, 1100, 1369 |
| Sau96I | GGNCC | 5 cut(s) | 542, 556, 557, 685, 1195 |
| ScaI | AGTACT | 2 cut(s) | 454, 1115 |
| SchI | GAGTC | 1 cut(s) | 1526 |
| ScrFI | CCNGG | 3 cut(s) | 731, 1175, 1461 |
| SduI | GDGCHC | 4 cut(s) | 560, 1418, 1457, 1495 |
| SfaNI | GCATC | 3 cut(s) | 1369, 1514, 1523 |
| SfcI | CTRYAG | 1 cut(s) | 448 |
| Sfr274I | CTCGAG | 1 cut(s) | 1534 |
| Sfr303I | CCGCGG | 1 cut(s) | 173 |
| SgrAI | CRCCGGYG | 1 cut(s) | 140 |
| SgrBI | CCGCGG | 1 cut(s) | 173 |
| SinI | GGWCC | 2 cut(s) | 542, 1195 |
| SlaI | CTCGAG | 1 cut(s) | 1534 |
| SmlI | CTYRAG | 4 cut(s) | 122, 971, 1134, 1534 |
| SmoI | CTYRAG | 4 cut(s) | 122, 971, 1134, 1534 |
| Sse9I | AATT | 7 cut(s) | 673, 961, 1047, 1242, 1425, 1587, 1591 |
| SseBI | AGGCCT | 1 cut(s) | 128 |
| SspI | AATATT | 1 cut(s) | 856 |
| SspMI | CTAG | 4 cut(s) | 770, 789, 1343, 1518 |
| SstI | GAGCTC | 1 cut(s) | 1495 |
| StuI | AGGCCT | 1 cut(s) | 128 |
| StyD4I | CCNGG | 3 cut(s) | 729, 1173, 1459 |
| StyI | CCWWGG | 4 cut(s) | 114, 183, 1106, 1526 |
| TaaI | ACNGT | 4 cut(s) | 662, 903, 1187, 1318 |
| TaiI | ACGT | 1 cut(s) | 335 |
| TaqI | TCGA | 3 cut(s) | 13, 608, 1535 |
| TaqII | GACCGA | 1 cut(s) | 149 |
| TasI | AATT | 7 cut(s) | 673, 961, 1047, 1242, 1425, 1587, 1591 |
| TatI | WGTACW | 4 cut(s) | 452, 533, 1113, 1579 |
| TauI | GCSGC | 4 cut(s) | 8, 65, 68, 172 |
| TfiI | GAWTC | 1 cut(s) | 883 |
| Tru1I | TTAA | 3 cut(s) | 852, 972, 1590 |
| Tru9I | TTAA | 3 cut(s) | 852, 972, 1590 |
| TscAI | CASTG | 2 cut(s) | 906, 1059 |
| TseFI | GTSAC | 1 cut(s) | 1159 |
| TseI | GCWGC | 6 cut(s) | 135, 285, 519, 565, 1447, 1511 |
| Tsp45I | GTSAC | 1 cut(s) | 1159 |
| TspDTI | ATGAA | 5 cut(s) | 248, 725, 1110, 1288, 1352 |
| TspGWI | ACGGA | 2 cut(s) | 44, 474 |
| TspRI | CASTG | 2 cut(s) | 906, 1059 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1268 |
| Vha464I | CTTAAG | 1 cut(s) | 971 |
| VpaK11BI | GGWCC | 2 cut(s) | 542, 1195 |
| VspI | ATTAAT | 1 cut(s) | 1590 |
| XapI | RAATTY | 2 cut(s) | 961, 1242 |
| XceI | RCATGY | 2 cut(s) | 512, 951 |
| XhoI | CTCGAG | 1 cut(s) | 1534 |
| XspI | CTAG | 4 cut(s) | 770, 789, 1343, 1518 |
| ZrmI | AGTACT | 2 cut(s) | 454, 1115 |