MD15G1362800.v1.1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001477
Physical Location & Seq
Reverse (-)
43800230 .. 43813024
12795 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1362800.v1.1.491

Sequence Viewer

Length: 1590 bp
ATGGAGAATCGAACTCTTATTTTACTACTATCCTTCTGCCTCTTCGCAGAAGCTTCACTTTCCCTCACATTGTCGTCGAAGCTCGTCCACCGCTTCTCGGAGGAGGCCATGGCGGTTTGGGAGTCCAGAAGCGGCGGAAACGCGTCCCTGAAGACATGGCCTAAGAGGAACAGCTTCAACTACTTTCAGCTTCTTCTGAAAAGCGATCTAAAGCGGCACAGACTGAAGCTGGGGTCGAAACGCGATCTTCTGATTCCTTCTCAAGGAAGCCAGACATTGTTCTTCGGAAACGAGCTTGATTGGTTGCATTATGCTTGGATTGACATAGGGACACCGAATGTTTCGTTTATGGTTGCATTGGATGGTGGGAGCGATTTGCTTTGGGTTCCATGTGATTGCATACAATGTGCCCCTCTGTCTGCCTCCAATTATGATTCTTTGGATAGGGATCTGAATCAGTATAGACCATCTTTATCAAGTACCAGCAAACAACTGAGTTGCAGCCATCAGTTATGCAAATCGAGCACGAATTGCAAAGGTCCAGCAGAACCTTGCCCTTACATTGTTCAGTACAAGTCAGAAAATACTGCAAGCTCTGGATTTCTGATTGAGGATGTTTTACATTTGACATCTGCCAGCAAAAATACAGCACCTAGTTCTGTGCAGGCTCCAGTCATCGTTGGCTGTGGTAGGAAACAAACTGGTGGCTATTTGGAAGGAGCTGCTCCTGATGGTGTCATGGGTTTGGGACCTGGGGAGATTTCGGTTCCGAGCGTACTTGCAAAAGCAGGAATGATCCAGAATTCATTTTCACTTTGTTTTGATGCCAATGGTTCGGGGAGAATTCTCTTTGGTGACCAAGGACATCTTGCCCAACAATCTACTCCTTTCTTGCCTATGGAAGAAAAGTATGTTGCGTATTCTGTTGGAGTAGAGCAATGTTGTGTTGCGCGTTCCTGTCTGAAGCAAACTGGATTCCAAGCTCTGGTTGACAGCGGCTCATCTTTTACTTATGTTCCAACTGAAGTTTATGAAAAAATTGTATTTGAGTTTGACAAGCAAGTGAATGCTACTAGAATTGACCTCCAACAGTCGCCTTGGAAGTACTGCTATAATGTCAGTTCATTGGAGTTGCTTAACATTCCTACTATGAAACTCATGTTCCCTCTGAACCAAAGCTTTCTAGTCAATAAGCCAATATTTTCTGAGACTCTGGATCAGAAATATACGATATTCTGTTTAACTTTATTGCGGACAGATGATGAGTATGGCATCATTGGACAGAACTTCATGGTGGGTTATCGCCTGGTTTTCGACAGGGAAAATCTGAAGTTTGGTTGGTCCAATGCCAATTGTGAAAATAATAATCACGGTAAGAAAGTTAATCAGGCGCCATCAACTAACGGGTCAACAAGCCAACTCCCAGTCCCAACCAGTGAGCAACAGAACATCTCCAACCCTCACACCGTTGCACCGGCCATTGCCAGGAAGGCACCCTCCAAACCCTCTGCAGCAGCATCCAGGCAAATCCCTTTTCTGCTCTGCTTGACCAGTTCTTTACTTTTGCTAATGTGTCTATTAAATCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TAXi_N

Protein Analysis

530

Amino Acids

58.27

Weight (kDa)

7.44

Isoelectric Point (pI)

42.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 104 - 448 2.6e-21 Eukaryotic aspartyl protease
TAXi_N PF14543 105 - 286 2e-38 Xylanase inhibitor N-terminal
TAXi_C PF14541 323 - 448 2.3e-13 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1390, 1492
AccB7I CCANNNNNTGG 1 cut(s) 985
AccII CGCG 3 cut(s) 143, 243, 952
AciI CCGC 7 cut(s) 91, 113, 132, 135, 214, 996, 1252
AclWI GGATC 3 cut(s) 456, 790, 1224
AcoI YGGCCR 1 cut(s) 1476
AcsI RAATTY 2 cut(s) 802, 843
AcuI CTGAAG 5 cut(s) 170, 245, 983, 1044, 1349
AcyI GRCGYC 1 cut(s) 1391
AfaI GTAC 4 cut(s) 481, 572, 777, 1106
AfiI CCNNNNNNNGG 4 cut(s) 97, 263, 985, 1485
AflIII ACRYGT 1 cut(s) 141
AgsI TTSAA 1 cut(s) 178
AjnI CCWGG 4 cut(s) 751, 1305, 1484, 1520
Alw21I GWGCWC 1 cut(s) 527
Alw26I GTCTC 1 cut(s) 1202
AlwI GGATC 3 cut(s) 456, 790, 1224
AoxI GGCC 3 cut(s) 105, 158, 1476
ApeKI GCWGC 4 cut(s) 501, 722, 1511, 1514
ApoI RAATTY 2 cut(s) 802, 843
Asp700I GAANNNNTTC 1 cut(s) 173
AspLEI GCGC 2 cut(s) 952, 1393
AspS9I GGNCC 3 cut(s) 539, 749, 1341
AsuHPI GGTGA 1 cut(s) 866
AvaII GGWCC 3 cut(s) 539, 749, 1341
BaeGI GKGCMC 1 cut(s) 412
BanI GGYRCC 2 cut(s) 1390, 1492
BbsI GAAGAC 1 cut(s) 158
Bbv12I GWGCWC 1 cut(s) 527
BbvI GCAGC 4 cut(s) 513, 709, 1523, 1526
BccI CCATC 5 cut(s) 356, 475, 513, 725, 1402
BciT130I CCWGG 4 cut(s) 753, 1307, 1486, 1522
BcoDI GTCTC 1 cut(s) 1202
BfaI CTAG 3 cut(s) 654, 1074, 1184
BfmI CTRYAG 1 cut(s) 1509
BfoI RGCGCY 1 cut(s) 1394
BglI GCCNNNNNGGC 1 cut(s) 1490
BisI GCNGC 7 cut(s) 133, 215, 502, 723, 997, 1512, 1515
BlsI GCNGC 7 cut(s) 134, 216, 503, 724, 998, 1513, 1516
BmcAI AGTACT 1 cut(s) 1106
Bme1390I CCNGG 4 cut(s) 753, 1307, 1486, 1522
Bme18I GGWCC 3 cut(s) 539, 749, 1341
BmgT120I GGNCC 3 cut(s) 539, 749, 1341
BmiI GGNNCC 6 cut(s) 387, 669, 750, 768, 1392, 1494
BmrFI CCNGG 4 cut(s) 753, 1307, 1486, 1522
BmrI ACTGGG 1 cut(s) 1418
BmsI GCATC 3 cut(s) 814, 1281, 1526
BmuI ACTGGG 1 cut(s) 1418
BpiI GAAGAC 1 cut(s) 158
BpmI CTGGAG 1 cut(s) 654
BpuEI CTTGAG 1 cut(s) 246
BsaBI GATNNNNATC 2 cut(s) 447, 453
BsaHI GRCGYC 1 cut(s) 1391
BsaJI CCNNGG 4 cut(s) 108, 752, 859, 1097
Bsc4I CCNNNNNNNGG 4 cut(s) 97, 263, 985, 1485
Bse118I RCCGGY 1 cut(s) 1474
Bse1I ACTGG 6 cut(s) 671, 706, 976, 1424, 1434, 1551
Bse3DI GCAATG 2 cut(s) 944, 1479
Bse8I GATNNNNATC 2 cut(s) 447, 453
BseBI CCWGG 4 cut(s) 753, 1307, 1486, 1522
BseDI CCNNGG 4 cut(s) 108, 752, 859, 1097
BseGI GGATG 3 cut(s) 367, 619, 1517
BseJI GATNNNNATC 2 cut(s) 447, 453
BseLI CCNNNNNNNGG 4 cut(s) 97, 263, 985, 1485
BseMI GCAATG 2 cut(s) 944, 1479
BseMII CTCAG 2 cut(s) 485, 1197
BseNI ACTGG 6 cut(s) 671, 706, 976, 1424, 1434, 1551
BseRI GAGGAG 1 cut(s) 116
BseSI GKGCMC 1 cut(s) 412
BseXI GCAGC 4 cut(s) 513, 709, 1523, 1526
BseYI CCCAGC 1 cut(s) 229
BsgI GTGCAG 1 cut(s) 683
Bsh1236I CGCG 3 cut(s) 143, 243, 952
BshFI GGCC 3 cut(s) 107, 160, 1478
BshNI GGYRCC 2 cut(s) 1390, 1492
BsiHKAI GWGCWC 1 cut(s) 527
BsiSI CCGG 1 cut(s) 1475
BslFI GGGAC 4 cut(s) 130, 343, 762, 1412
BslI CCNNNNNNNGG 4 cut(s) 97, 263, 985, 1485
BsmAI GTCTC 1 cut(s) 1202
BsmFI GGGAC 4 cut(s) 130, 343, 762, 1412
BsmI GAATGC 1 cut(s) 1072
BsnI GGCC 3 cut(s) 107, 160, 1478
Bsp1286I GDGCHC 2 cut(s) 412, 527
Bsp143I GATC 5 cut(s) 205, 244, 448, 795, 1216
Bsp19I CCATGG 1 cut(s) 108
BspACI CCGC 7 cut(s) 91, 113, 132, 135, 214, 996, 1252
BspANI GGCC 3 cut(s) 107, 160, 1478
BspCNI CTCAG 2 cut(s) 486, 1198
BspFNI CGCG 3 cut(s) 143, 243, 952
BspLI GGNNCC 6 cut(s) 387, 669, 750, 768, 1392, 1494
BspMAI CTGCAG 1 cut(s) 1513
BspPI GGATC 3 cut(s) 456, 790, 1224
BspT107I GGYRCC 2 cut(s) 1390, 1492
BsrDI GCAATG 2 cut(s) 944, 1479
BsrFI RCCGGY 1 cut(s) 1474
BsrI ACTGG 6 cut(s) 671, 706, 976, 1424, 1434, 1551
BssAI RCCGGY 1 cut(s) 1474
BssECI CCNNGG 4 cut(s) 108, 752, 859, 1097
BssMI GATC 5 cut(s) 205, 244, 448, 795, 1216
BssNI GRCGYC 1 cut(s) 1391
BssT1I CCWWGG 3 cut(s) 108, 859, 1097
Bst2UI CCWGG 4 cut(s) 753, 1307, 1486, 1522
Bst4CI ACNGT 3 cut(s) 1092, 1373, 1468
Bst6I CTCTTC 1 cut(s) 47
BstACI GRCGYC 1 cut(s) 1391
BstAPI GCANNNNNTGC 1 cut(s) 531
BstC8I GCNNGC 3 cut(s) 592, 637, 666
BstDEI CTNAG 3 cut(s) 162, 494, 1206
BstDSI CCRYGG 1 cut(s) 108
BstEII GGTNACC 1 cut(s) 854
BstENI CCTNNNNNAGG 1 cut(s) 261
BstF5I GGATG 3 cut(s) 367, 619, 1517
BstFNI CGCG 3 cut(s) 143, 243, 952
BstH2I RGCGCY 1 cut(s) 1394
BstHHI GCGC 2 cut(s) 952, 1393
BstKTI GATC 5 cut(s) 208, 247, 451, 798, 1219
BstMAI GTCTC 1 cut(s) 1202
BstMBI GATC 5 cut(s) 205, 244, 448, 795, 1216
BstMWI GCNNNNNNNGC 3 cut(s) 522, 531, 1490
BstNI CCWGG 4 cut(s) 753, 1307, 1486, 1522
BstPI GGTNACC 1 cut(s) 854
BstSCI CCNGG 4 cut(s) 751, 1305, 1484, 1520
BstSFI CTRYAG 1 cut(s) 1509
BstSLI GKGCMC 1 cut(s) 412
BstUI CGCG 3 cut(s) 143, 243, 952
BstV1I GCAGC 4 cut(s) 513, 709, 1523, 1526
BstV2I GAAGAC 1 cut(s) 158
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
BsuRI GGCC 3 cut(s) 107, 160, 1478
BtgI CCRYGG 1 cut(s) 108
BtsCI GGATG 3 cut(s) 367, 619, 1517
BtsIMutI CAGTG 1 cut(s) 1441
Cac8I GCNNGC 3 cut(s) 592, 637, 666
CfoI GCGC 2 cut(s) 952, 1393
Cfr10I RCCGGY 1 cut(s) 1474
Cfr13I GGNCC 3 cut(s) 539, 749, 1341
CseI GACGC 1 cut(s) 132
Csp6I GTAC 4 cut(s) 480, 571, 776, 1105
CviAII CATG 6 cut(s) 109, 156, 390, 739, 1159, 1291
CviQI GTAC 4 cut(s) 480, 571, 776, 1105
DdeI CTNAG 3 cut(s) 162, 494, 1206
DinI GGCGCC 1 cut(s) 1392
DpnI GATC 5 cut(s) 207, 246, 450, 797, 1218
DpnII GATC 5 cut(s) 205, 244, 448, 795, 1216
EaeI YGGCCR 1 cut(s) 1476
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
EciI GGCGGA 1 cut(s) 150
Eco130I CCWWGG 3 cut(s) 108, 859, 1097
Eco47I GGWCC 3 cut(s) 539, 749, 1341
Eco57I CTGAAG 5 cut(s) 170, 245, 983, 1044, 1349
Eco91I GGTNACC 1 cut(s) 854
EcoNI CCTNNNNNAGG 1 cut(s) 261
EcoO109I RGGNCCY 1 cut(s) 749
EcoO65I GGTNACC 1 cut(s) 854
EcoRI GAATTC 2 cut(s) 802, 843
EcoRII CCWGG 4 cut(s) 751, 1305, 1484, 1520
EcoT14I CCWWGG 3 cut(s) 108, 859, 1097
EgeI GGCGCC 1 cut(s) 1392
EheI GGCGCC 1 cut(s) 1392
ErhI CCWWGG 3 cut(s) 108, 859, 1097
FaeI CATG 6 cut(s) 112, 159, 393, 742, 1162, 1294
FalI AAGNNNNNCTT 4 cut(s) 42, 74, 852, 884
FaqI GGGAC 4 cut(s) 130, 343, 762, 1412
FatI CATG 6 cut(s) 108, 155, 389, 738, 1158, 1290
Fnu4HI GCNGC 7 cut(s) 133, 215, 502, 723, 997, 1512, 1515
FokI GGATG 3 cut(s) 374, 626, 1504
Fsp4HI GCNGC 7 cut(s) 133, 215, 502, 723, 997, 1512, 1515
FspBI CTAG 3 cut(s) 654, 1074, 1184
GlaI GCGC 2 cut(s) 951, 1392
GluI GCNGC 7 cut(s) 133, 215, 502, 723, 997, 1512, 1515
GsaI CCCAGC 1 cut(s) 233
GsuI CTGGAG 1 cut(s) 654
HaeII RGCGCY 1 cut(s) 1394
HaeIII GGCC 3 cut(s) 107, 160, 1478
HapII CCGG 1 cut(s) 1475
HgaI GACGC 1 cut(s) 132
HhaI GCGC 2 cut(s) 952, 1393
Hin1I GRCGYC 1 cut(s) 1391
Hin1II CATG 6 cut(s) 112, 159, 393, 742, 1162, 1294
Hin6I GCGC 2 cut(s) 950, 1391
HinP1I GCGC 2 cut(s) 950, 1391
HincII GTYRAC 2 cut(s) 991, 1410
HindII GTYRAC 2 cut(s) 991, 1410
HindIII AAGCTT 2 cut(s) 51, 1177
HinfI GANTC 7 cut(s) 7, 122, 253, 434, 454, 975, 1210
HpaII CCGG 1 cut(s) 1475
HphI GGTGA 1 cut(s) 866
Hpy166II GTNNAC 3 cut(s) 88, 991, 1410
Hpy188III TCNNGA 5 cut(s) 126, 597, 728, 799, 1214
Hpy8I GTNNAC 3 cut(s) 88, 991, 1410
Hpy99I CGWCG 1 cut(s) 79
HpyAV CCTTC 4 cut(s) 43, 267, 710, 1483
HpyCH4III ACNGT 3 cut(s) 1092, 1373, 1468
HpyF10VI GCNNNNNNNGC 3 cut(s) 522, 531, 1490
HpyF3I CTNAG 3 cut(s) 162, 494, 1206
Hsp92I GRCGYC 1 cut(s) 1391
Hsp92II CATG 6 cut(s) 112, 159, 393, 742, 1162, 1294
HspAI GCGC 2 cut(s) 950, 1391
KasI GGCGCC 1 cut(s) 1390
Kzo9I GATC 5 cut(s) 205, 244, 448, 795, 1216
LmnI GCTCC 4 cut(s) 369, 673, 719, 730
Lsp1109I GCAGC 4 cut(s) 513, 709, 1523, 1526
LweI GCATC 3 cut(s) 814, 1281, 1526
MaeI CTAG 3 cut(s) 654, 1074, 1184
MaeIII GTNAC 1 cut(s) 854
MalI GATC 5 cut(s) 207, 246, 450, 797, 1218
MboI GATC 5 cut(s) 205, 244, 448, 795, 1216
MboII GAAGA 6 cut(s) 34, 163, 185, 239, 274, 914
MfeI CAATTG 1 cut(s) 1351
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 2 cut(s) 412, 527
MluCI AATT 7 cut(s) 427, 529, 802, 843, 1038, 1077, 1351
MluI ACGCGT 1 cut(s) 141
Mly113I GGCGCC 1 cut(s) 1391
MlyI GAGTC 2 cut(s) 131, 1204
MmeI TCCRAC 4 cut(s) 907, 1043, 1111, 1479
MroXI GAANNNNTTC 1 cut(s) 173
MseI TTAA 5 cut(s) 1137, 1241, 1383, 1580, 1588
MspA1I CMGCKG 1 cut(s) 996
MspI CCGG 1 cut(s) 1475
MspR9I CCNGG 4 cut(s) 753, 1307, 1486, 1522
MunI CAATTG 1 cut(s) 1351
Mva1269I GAATGC 1 cut(s) 1072
MvaI CCWGG 4 cut(s) 753, 1307, 1486, 1522
MvnI CGCG 3 cut(s) 143, 243, 952
MwoI GCNNNNNNNGC 3 cut(s) 522, 531, 1490
NarI GGCGCC 1 cut(s) 1391
NcoI CCATGG 1 cut(s) 108
NdeII GATC 5 cut(s) 205, 244, 448, 795, 1216
NlaIII CATG 6 cut(s) 112, 159, 393, 742, 1162, 1294
NlaIV GGNNCC 6 cut(s) 387, 669, 750, 768, 1392, 1494
NmuCI GTSAC 1 cut(s) 854
PctI GAATGC 1 cut(s) 1072
PdmI GAANNNNTTC 1 cut(s) 173
PfeI GAWTC 5 cut(s) 7, 253, 434, 454, 975
PflMI CCANNNNNTGG 1 cut(s) 985
PkrI GCNGC 7 cut(s) 134, 216, 503, 724, 998, 1513, 1516
PleI GAGTC 2 cut(s) 130, 1204
PluTI GGCGCC 1 cut(s) 1394
PpsI GAGTC 2 cut(s) 130, 1204
PpuMI RGGWCCY 1 cut(s) 749
Psp5II RGGWCCY 1 cut(s) 749
Psp6I CCWGG 4 cut(s) 751, 1305, 1484, 1520
PspEI GGTNACC 1 cut(s) 854
PspFI CCCAGC 1 cut(s) 229
PspGI CCWGG 4 cut(s) 751, 1305, 1484, 1520
PspN4I GGNNCC 6 cut(s) 387, 669, 750, 768, 1392, 1494
PspPI GGNCC 3 cut(s) 539, 749, 1341
PspPPI RGGWCCY 1 cut(s) 749
PstI CTGCAG 1 cut(s) 1513
PsuI RGATCY 1 cut(s) 448
RsaI GTAC 4 cut(s) 481, 572, 777, 1106
RsaNI GTAC 4 cut(s) 480, 571, 776, 1105
SaqAI TTAA 5 cut(s) 1137, 1241, 1383, 1580, 1588
SatI GCNGC 7 cut(s) 133, 215, 502, 723, 997, 1512, 1515
Sau3AI GATC 5 cut(s) 205, 244, 448, 795, 1216
Sau96I GGNCC 3 cut(s) 539, 749, 1341
ScaI AGTACT 1 cut(s) 1106
SchI GAGTC 2 cut(s) 131, 1204
ScrFI CCNGG 4 cut(s) 753, 1307, 1486, 1522
SduI GDGCHC 2 cut(s) 412, 527
SfaNI GCATC 3 cut(s) 814, 1281, 1526
SfcI CTRYAG 1 cut(s) 1509
SfoI GGCGCC 1 cut(s) 1392
SinI GGWCC 3 cut(s) 539, 749, 1341
SmlI CTYRAG 1 cut(s) 261
SmoI CTYRAG 1 cut(s) 261
Sse9I AATT 7 cut(s) 427, 529, 802, 843, 1038, 1077, 1351
SsiI CCGC 7 cut(s) 91, 113, 132, 135, 214, 996, 1252
SspDI GGCGCC 1 cut(s) 1390
SspI AATATT 1 cut(s) 1200
SspMI CTAG 3 cut(s) 654, 1074, 1184
StyD4I CCNGG 4 cut(s) 751, 1305, 1484, 1520
StyI CCWWGG 3 cut(s) 108, 859, 1097
TaaI ACNGT 3 cut(s) 1092, 1373, 1468
TaqI TCGA 5 cut(s) 10, 77, 236, 521, 1314
TasI AATT 7 cut(s) 427, 529, 802, 843, 1038, 1077, 1351
TatI WGTACW 2 cut(s) 570, 1104
TauI GCSGC 3 cut(s) 135, 217, 999
TfiI GAWTC 5 cut(s) 7, 253, 434, 454, 975
Tru1I TTAA 5 cut(s) 1137, 1241, 1383, 1580, 1588
Tru9I TTAA 5 cut(s) 1137, 1241, 1383, 1580, 1588
TscAI CASTG 1 cut(s) 1441
TseFI GTSAC 1 cut(s) 854
TseI GCWGC 4 cut(s) 501, 722, 1511, 1514
Tsp45I GTSAC 1 cut(s) 854
TspDTI ATGAA 5 cut(s) 795, 1047, 1113, 1166, 1279
TspRI CASTG 1 cut(s) 1441
Van91I CCANNNNNTGG 1 cut(s) 985
VpaK11BI GGWCC 3 cut(s) 539, 749, 1341
XagI CCTNNNNNAGG 1 cut(s) 261
XapI RAATTY 2 cut(s) 802, 843
XmnI GAANNNNTTC 1 cut(s) 173
XspI CTAG 3 cut(s) 654, 1074, 1184
ZrmI AGTACT 1 cut(s) 1106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.