Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0001287

Physical Location & Seq

Genomic Coordinates Reverse (-)

22383221 .. 22386660

Gene Structure 3440 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1111300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1197 bp

ATGGCTTTAGCAAAAACAATTATGGATCAAATGGAGGCGCCAATTCTAGATGCCTTCGACGGTGGAACAGTAGATTTTGCCGCCAATGGTGCAGAAGCAGAGTCTTTCAAGCACCGGGTTGATGAAATTTTCGTAAATGTTGATAAGCTCGAGGAAAGAGTGAATGACATAGAGCAGTTTTACGCGACTACGAGTAAAAAGATGCCAAATGCGTGTAAAAGTAGCTCAACTGCAAAGGATAAAGATCAGACAAAACATGTTCCAAGTATTAAGAAGCAGCAGCAAGATGCAGCATGTAGGGAAGCAGCTGCTGCTAAGAGAATGCAGGAACTTATGCGCCAGTTTGGCACGATATTGCGTCAGATTGCTCAGCACAAGTGGGCGTGGCCTTTTTTGCAGCCTGTAGATGTAGAAGGTCTTGGTTTAGATGACTATTATAAGGTGATTGACAAACCAATGGATTTAGGCACTATTAAAAATCAAATGGAGGCCAAAGATGGTACTGGATATAAACATGTTCGGGAGATATTTGCTGATGTGAGGTTGGTTTTTAAAAATGCGATGAAGTATAATGATGACAGAAGTGATGTTCATGTTATGGCCAAAACTTTGATGGCAAAATTTGAGGAGAAGTGGCTCCAGTTTCTGCCTAAAGTTACGGAGGAGGAAAAAAGGCTAGAAGAGGAGGAGGCAGAAGCACAGTTAAATATGCAACTTGCTGAAGATGCTGCTCGTGCCAAAATGGCTAGAGATTTAAGTAACGAGCTTTATGAGATTGATATGCATTTGGAAGAACTCCGCGAAATGGTTGTTCAGAAGTGCAGAAAAATGTCTACTGAAGAGAAGCGAAAGCTTGGGGTAGCCCTTACCAGATTGTCGCCTGAGGATCTTAGCAAGGCATTGGAGATTGTTGCGCAAAGTAATCCAGGTTTCCAGGCTACTGCCGATGAGGTGGACCTTGACATCGATGCTCAGACTGAGTCTACCTTGTGGAGGTTGAAGTTTTTTGTGAGGGATGCTATTGAAGTTCAAAGCAAGAGTCCAGCAAGCATGGGCGGCAACACCAACAACGCTAATAACCACAACAGTGGAAACAAAACTAACAACATCAGCAACGCTACTAATAAGCGAAAAAGAGAGATTTCTGATGCGATCGTCAAAACTGCCAAGAAAAAGAGTAAAAAGCCATCTCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010029 GO:0010030 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0048580 GO:0048582 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:1900140 GO:1902680 GO:1903506 GO:1903508 GO:2000026 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BET Bromodomain

Protein Analysis

399

Amino Acids

45.11

Weight (kDa)

6.09

Isoelectric Point (pI)

53.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Bromodomain	PF00439	117 - 202	2.7e-17	Bromodomain
BET	PF17035	276 - 338	7.3e-19	Bromodomain extra-terminal - transcription regulation

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001287)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G34900 AT2G34900
fragaria_vesca	FvH4_3g13380 FvH4_3g13380 FvH4_3g13380 FvH4_3g13380 FvH4_3g13380 FvH4_3g13380 FvH4_3g13380 FvH4_7g19980 FvH4_7g19980 FvH4_7g19980 FvH4_7g19980 FvH4_7g19980 FvH4_7g19980
malus_domestica	MD01G1111300.v1.1 MD06G1168600.v1.1 MD08G1151100.v1.1 MD08G1172000.v1.1 MD10G1224000.v1.1 MD12G1128700.v1.1 MD13G1214300.v1.1
prunus_persica	Prupe.2G216800_v2.0.a1 Prupe.2G216800_v2.0.a1 Prupe.2G216800_v2.0.a1 Prupe.4G121200_v2.0.a1 Prupe.4G121200_v2.0.a1 Prupe.4G121200_v2.0.a1 Prupe.4G121200_v2.0.a1 Prupe.4G121200_v2.0.a1 Prupe.4G121200_v2.0.a1 Prupe.4G121200_v2.0.a1
pyrus_communis	pycom01g13870 pycom10g18800
rosa_chinensis	RchiOBHm_Chr1g0363941 RchiOBHm_Chr5g0022091
rosa_laevigata	RLG00000032643
rosa_multiflora	Rmu_co8422649.1_g000001 Rmu_sc0012801.1_g000001
rosa_roxburghii	Rroxscaffold_1G00056840 Rroxscaffold_4G00292820
rosa_rugosa	Rorug01G0311400 Rorug01G0311500 Rorug05G0071200
rosa_samantha	Rh1AG319300 Rh1BG282300 Rh1CG298800 Rh1DG313700 Rh5AG159100 Rh5BG158400 Rh5CG172800 Rh5DG158600
rosa_wichuraiana	Rw1G028300 Rw1G028310 Rw5G014200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	438
Acc16I	TGCGCA	1 cut(s)	915
AccB1I	GGYRCC	1 cut(s)	37
AccI	GTMKAC	2 cut(s)	833, 983
AccII	CGCG	2 cut(s)	185, 801
AciI	CCGC	3 cut(s)	81, 799, 1056
AclWI	GGATC	2 cut(s)	33, 894
AcoI	YGGCCR	1 cut(s)	600
AcsI	RAATTY	2 cut(s)	126, 620
AcuI	CTGAAG	2 cut(s)	741, 858
AcyI	GRCGYC	1 cut(s)	38
AfaI	GTAC	1 cut(s)	502
AfiI	CCNNNNNNNGG	2 cut(s)	805, 993
AflIII	ACRYGT	2 cut(s)	256, 514
AgsI	TTSAA	4 cut(s)	109, 1000, 1025, 1031
AjnI	CCWGG	2 cut(s)	925, 933
AleI	CACNNNNGTG	1 cut(s)	1086
AluBI	AGCT	5 cut(s)	148, 225, 308, 766, 853
AluI	AGCT	5 cut(s)	148, 225, 308, 766, 853
AlwI	GGATC	2 cut(s)	33, 894
AlwNI	CAGNNNCTG	2 cut(s)	311, 646
Ama87I	CYCGRG	1 cut(s)	149
AoxI	GGCC	3 cut(s)	386, 489, 600
ApeKI	GCWGC	8 cut(s)	277, 280, 290, 305, 308, 311, 397, 728
ApoI	RAATTY	2 cut(s)	126, 620
ArsI	GACNNNNNNTTYG	2 cut(s)	1024, 1056
AspLEI	GCGC	3 cut(s)	40, 339, 916
AspS9I	GGNCC	1 cut(s)	955
AsuC2I	CCSGG	1 cut(s)	116
AsuHPI	GGTGA	1 cut(s)	454
AvaI	CYCGRG	1 cut(s)	149
AvaII	GGWCC	1 cut(s)	955
AxyI	CCTNAGG	1 cut(s)	882
BalI	TGGCCA	1 cut(s)	602
BanI	GGYRCC	1 cut(s)	37
BauI	CACGAG	1 cut(s)	732
BbvI	GCAGC	8 cut(s)	289, 292, 295, 298, 302, 317, 409, 715
BccI	CCATC	3 cut(s)	491, 607, 1195
BciT130I	CCWGG	2 cut(s)	927, 935
BcnI	CCSGG	1 cut(s)	116
BfaI	CTAG	3 cut(s)	47, 677, 747
BfmI	CTRYAG	1 cut(s)	402
BfoI	RGCGCY	1 cut(s)	41
BglI	GCCNNNNNGGC	2 cut(s)	345, 743
BlpI	GCTNAGC	1 cut(s)	369
Bme1390I	CCNGG	3 cut(s)	116, 927, 935
Bme18I	GGWCC	1 cut(s)	955
BmeT110I	CYCGRG	1 cut(s)	149
BmgT120I	GGNCC	1 cut(s)	955
BmiI	GGNNCC	2 cut(s)	39, 638
BmrFI	CCNGG	3 cut(s)	116, 927, 935
BmsI	GCATC	7 cut(s)	40, 192, 277, 715, 958, 1006, 1138
BpmI	CTGGAG	1 cut(s)	623
Bpu1102I	GCTNAGC	1 cut(s)	369
BpuMI	CCSGG	1 cut(s)	116
Bsa29I	ATCGAT	1 cut(s)	966
BsaBI	GATNNNNATC	1 cut(s)	243
BsaHI	GRCGYC	1 cut(s)	38
Bsc4I	CCNNNNNNNGG	2 cut(s)	805, 993
Bse1I	ACTGG	3 cut(s)	340, 508, 640
Bse21I	CCTNAGG	1 cut(s)	882
Bse8I	GATNNNNATC	1 cut(s)	243
BseBI	CCWGG	2 cut(s)	927, 935
BseCI	ATCGAT	1 cut(s)	966
BseGI	GGATG	1 cut(s)	1021
BseJI	GATNNNNATC	1 cut(s)	243
BseLI	CCNNNNNNNGG	2 cut(s)	805, 993
BseMII	CTCAG	4 cut(s)	383, 873, 969, 986
BseNI	ACTGG	3 cut(s)	340, 508, 640
BseRI	GAGGAG	4 cut(s)	641, 677, 698, 701
BseXI	GCAGC	8 cut(s)	289, 292, 295, 298, 302, 317, 409, 715
BsgI	GTGCAG	2 cut(s)	111, 841
Bsh1236I	CGCG	2 cut(s)	185, 801
Bsh1285I	CGRYCG	1 cut(s)	1155
BshFI	GGCC	3 cut(s)	388, 491, 602
BshNI	GGYRCC	1 cut(s)	37
BshVI	ATCGAT	1 cut(s)	966
BsiEI	CGRYCG	1 cut(s)	1155
BsiHKCI	CYCGRG	1 cut(s)	149
BsiSI	CCGG	1 cut(s)	115
BslI	CCNNNNNNNGG	2 cut(s)	805, 993
BsmI	GAATGC	1 cut(s)	327
BsnI	GGCC	3 cut(s)	388, 491, 602
BsoBI	CYCGRG	1 cut(s)	149
Bsp143I	GATC	4 cut(s)	25, 244, 886, 1152
Bsp1720I	GCTNAGC	1 cut(s)	369
BspACI	CCGC	3 cut(s)	81, 799, 1056
BspANI	GGCC	3 cut(s)	388, 491, 602
BspCNI	CTCAG	4 cut(s)	382, 874, 970, 985
BspDI	ATCGAT	1 cut(s)	966
BspFNI	CGCG	2 cut(s)	185, 801
BspLI	GGNNCC	2 cut(s)	39, 638
BspPI	GGATC	2 cut(s)	33, 894
BspT107I	GGYRCC	1 cut(s)	37
BsrI	ACTGG	3 cut(s)	340, 508, 640
BssMI	GATC	4 cut(s)	25, 244, 886, 1152
BssNI	GRCGYC	1 cut(s)	38
BssSI	CACGAG	1 cut(s)	732
Bst2BI	CACGAG	1 cut(s)	732
Bst2UI	CCWGG	2 cut(s)	927, 935
Bst4CI	ACNGT	4 cut(s)	62, 70, 702, 1088
Bst6I	CTCTTC	2 cut(s)	675, 834
BstACI	GRCGYC	1 cut(s)	38
BstAPI	GCANNNNNTGC	1 cut(s)	311
BstC8I	GCNNGC	1 cut(s)	1048
BstDEI	CTNAG	6 cut(s)	315, 369, 882, 890, 972, 978
BstENI	CCTNNNNNAGG	1 cut(s)	991
BstF5I	GGATG	1 cut(s)	1021
BstFNI	CGCG	2 cut(s)	185, 801
BstH2I	RGCGCY	1 cut(s)	41
BstHHI	GCGC	3 cut(s)	40, 339, 916
BstKTI	GATC	4 cut(s)	28, 247, 889, 1155
BstMBI	GATC	4 cut(s)	25, 244, 886, 1152
BstMCI	CGRYCG	1 cut(s)	1155
BstMWI	GCNNNNNNNGC	8 cut(s)	89, 311, 345, 394, 725, 734, 743, 1056
BstNI	CCWGG	2 cut(s)	927, 935
BstNSI	RCATGY	3 cut(s)	260, 297, 518
BstSCI	CCNGG	3 cut(s)	114, 925, 933
BstSFI	CTRYAG	1 cut(s)	402
BstUI	CGCG	2 cut(s)	185, 801
BstV1I	GCAGC	8 cut(s)	289, 292, 295, 298, 302, 317, 409, 715
BstX2I	RGATCY	1 cut(s)	886
BstXI	CCANNNNNNTGG	1 cut(s)	1088
BstYI	RGATCY	1 cut(s)	886
Bsu15I	ATCGAT	1 cut(s)	966
Bsu36I	CCTNAGG	1 cut(s)	882
BsuRI	GGCC	3 cut(s)	388, 491, 602
BsuTUI	ATCGAT	1 cut(s)	966
BtgZI	GCGATG	1 cut(s)	575
BtsCI	GGATG	1 cut(s)	1021
BtsIMutI	CAGTG	1 cut(s)	1093
Cac8I	GCNNGC	1 cut(s)	1048
CaiI	CAGNNNCTG	2 cut(s)	311, 646
CfoI	GCGC	3 cut(s)	40, 339, 916
Cfr13I	GGNCC	1 cut(s)	955
ClaI	ATCGAT	1 cut(s)	966
CseI	GACGC	1 cut(s)	347
Csp6I	GTAC	1 cut(s)	501
CviAII	CATG	5 cut(s)	257, 294, 515, 593, 1051
CviQI	GTAC	1 cut(s)	501
DdeI	CTNAG	6 cut(s)	315, 369, 882, 890, 972, 978
DinI	GGCGCC	1 cut(s)	39
DpnI	GATC	4 cut(s)	27, 246, 888, 1154
DpnII	GATC	4 cut(s)	25, 244, 886, 1152
DraI	TTTAAA	1 cut(s)	553
EaeI	YGGCCR	1 cut(s)	600
Eam1104I	CTCTTC	2 cut(s)	675, 834
EarI	CTCTTC	2 cut(s)	675, 834
Eco47I	GGWCC	1 cut(s)	955
Eco57I	CTGAAG	2 cut(s)	741, 858
Eco81I	CCTNAGG	1 cut(s)	882
Eco88I	CYCGRG	1 cut(s)	149
EcoNI	CCTNNNNNAGG	1 cut(s)	991
EcoRII	CCWGG	2 cut(s)	925, 933
EcoT22I	ATGCAT	1 cut(s)	786
EgeI	GGCGCC	1 cut(s)	39
EheI	GGCGCC	1 cut(s)	39
FaeI	CATG	5 cut(s)	260, 297, 518, 596, 1054
FatI	CATG	5 cut(s)	256, 293, 514, 592, 1050
FblI	GTMKAC	2 cut(s)	833, 983
FokI	GGATG	1 cut(s)	1028
FspBI	CTAG	3 cut(s)	47, 677, 747
FspI	TGCGCA	1 cut(s)	915
GlaI	GCGC	3 cut(s)	39, 338, 915
GsuI	CTGGAG	1 cut(s)	623
HaeII	RGCGCY	1 cut(s)	41
HaeIII	GGCC	3 cut(s)	388, 491, 602
HapII	CCGG	1 cut(s)	115
HgaI	GACGC	1 cut(s)	347
HhaI	GCGC	3 cut(s)	40, 339, 916
Hin1I	GRCGYC	1 cut(s)	38
Hin1II	CATG	5 cut(s)	260, 297, 518, 596, 1054
Hin6I	GCGC	3 cut(s)	38, 337, 914
HinP1I	GCGC	3 cut(s)	38, 337, 914
HindIII	AAGCTT	1 cut(s)	851
HinfI	GANTC	3 cut(s)	101, 980, 1039
HpaII	CCGG	1 cut(s)	115
HphI	GGTGA	1 cut(s)	454
Hpy166II	GTNNAC	3 cut(s)	834, 955, 984
Hpy188I	TCNGA	5 cut(s)	249, 363, 816, 975, 1147
Hpy188III	TCNNGA	2 cut(s)	47, 521
Hpy8I	GTNNAC	3 cut(s)	834, 955, 984
Hpy99I	CGWCG	1 cut(s)	62
HpyAV	CCTTC	2 cut(s)	64, 407
HpyCH4III	ACNGT	4 cut(s)	62, 70, 702, 1088
HpyCH4V	TGCA	8 cut(s)	92, 233, 290, 325, 397, 712, 784, 822
HpyF10VI	GCNNNNNNNGC	8 cut(s)	89, 311, 345, 394, 725, 734, 743, 1056
HpyF3I	CTNAG	6 cut(s)	315, 369, 882, 890, 972, 978
Hsp92I	GRCGYC	1 cut(s)	38
Hsp92II	CATG	5 cut(s)	260, 297, 518, 596, 1054
HspAI	GCGC	3 cut(s)	38, 337, 914
KasI	GGCGCC	1 cut(s)	37
Kzo9I	GATC	4 cut(s)	25, 244, 886, 1152
LmnI	GCTCC	1 cut(s)	642
Lsp1109I	GCAGC	8 cut(s)	289, 292, 295, 298, 302, 317, 409, 715
LweI	GCATC	7 cut(s)	40, 192, 277, 715, 958, 1006, 1138
MaeI	CTAG	3 cut(s)	47, 677, 747
MaeIII	GTNAC	2 cut(s)	655, 758
MalI	GATC	4 cut(s)	27, 246, 888, 1154
MboI	GATC	4 cut(s)	25, 244, 886, 1152
MboII	GAAGA	4 cut(s)	692, 734, 803, 851
MflI	RGATCY	1 cut(s)	886
MlsI	TGGCCA	1 cut(s)	602
MluCI	AATT	4 cut(s)	18, 42, 126, 620
MluNI	TGGCCA	1 cut(s)	602
Mly113I	GGCGCC	1 cut(s)	38
MlyI	GAGTC	3 cut(s)	110, 989, 1048
Mox20I	TGGCCA	1 cut(s)	602
Mph1103I	ATGCAT	1 cut(s)	786
MscI	TGGCCA	1 cut(s)	602
MseI	TTAA	5 cut(s)	270, 474, 552, 704, 755
MslI	CAYNNNNRTG	1 cut(s)	1086
Msp20I	TGGCCA	1 cut(s)	602
MspA1I	CMGCKG	1 cut(s)	308
MspI	CCGG	1 cut(s)	115
MspR9I	CCNGG	3 cut(s)	116, 927, 935
Mva1269I	GAATGC	1 cut(s)	327
MvaI	CCWGG	2 cut(s)	927, 935
MvnI	CGCG	2 cut(s)	185, 801
MwoI	GCNNNNNNNGC	8 cut(s)	89, 311, 345, 394, 725, 734, 743, 1056
NarI	GGCGCC	1 cut(s)	38
NciI	CCSGG	1 cut(s)	116
NdeII	GATC	4 cut(s)	25, 244, 886, 1152
NlaIII	CATG	5 cut(s)	260, 297, 518, 596, 1054
NlaIV	GGNNCC	2 cut(s)	39, 638
NsbI	TGCGCA	1 cut(s)	915
NsiI	ATGCAT	1 cut(s)	786
NspI	RCATGY	3 cut(s)	260, 297, 518
OliI	CACNNNNGTG	1 cut(s)	1086
PaeR7I	CTCGAG	1 cut(s)	149
PciI	ACATGT	2 cut(s)	256, 514
PctI	GAATGC	1 cut(s)	327
PflFI	GACNNNGTC	1 cut(s)	979
Ple19I	CGATCG	1 cut(s)	1155
PleI	GAGTC	3 cut(s)	109, 988, 1047
PluTI	GGCGCC	1 cut(s)	41
PpsI	GAGTC	3 cut(s)	109, 988, 1047
PscI	ACATGT	2 cut(s)	256, 514
PsiI	TTATAA	1 cut(s)	438
Psp6I	CCWGG	2 cut(s)	925, 933
PspGI	CCWGG	2 cut(s)	925, 933
PspN4I	GGNNCC	2 cut(s)	39, 638
PspPI	GGNCC	1 cut(s)	955
PspXI	VCTCGAGB	1 cut(s)	149
PstNI	CAGNNNCTG	2 cut(s)	311, 646
PsuI	RGATCY	1 cut(s)	886
PsyI	GACNNNGTC	1 cut(s)	979
PvuI	CGATCG	1 cut(s)	1155
PvuII	CAGCTG	1 cut(s)	308
RsaI	GTAC	1 cut(s)	502
RsaNI	GTAC	1 cut(s)	501
RseI	CAYNNNNRTG	1 cut(s)	1086
SaqAI	TTAA	5 cut(s)	270, 474, 552, 704, 755
Sau3AI	GATC	4 cut(s)	25, 244, 886, 1152
Sau96I	GGNCC	1 cut(s)	955
SchI	GAGTC	3 cut(s)	110, 989, 1048
ScrFI	CCNGG	3 cut(s)	116, 927, 935
SfaNI	GCATC	7 cut(s)	40, 192, 277, 715, 958, 1006, 1138
SfcI	CTRYAG	1 cut(s)	402
SfoI	GGCGCC	1 cut(s)	39
Sfr274I	CTCGAG	1 cut(s)	149
SinI	GGWCC	1 cut(s)	955
SlaI	CTCGAG	1 cut(s)	149
SmiMI	CAYNNNNRTG	1 cut(s)	1086
SmlI	CTYRAG	1 cut(s)	149
SmoI	CTYRAG	1 cut(s)	149
Sse9I	AATT	4 cut(s)	18, 42, 126, 620
SsiI	CCGC	3 cut(s)	81, 799, 1056
SspDI	GGCGCC	1 cut(s)	37
SspMI	CTAG	3 cut(s)	47, 677, 747
StyD4I	CCNGG	3 cut(s)	114, 925, 933
TaaI	ACNGT	4 cut(s)	62, 70, 702, 1088
TaqI	TCGA	3 cut(s)	57, 150, 966
TasI	AATT	4 cut(s)	18, 42, 126, 620
TauI	GCSGC	2 cut(s)	83, 1059
Tru1I	TTAA	5 cut(s)	270, 474, 552, 704, 755
Tru9I	TTAA	5 cut(s)	270, 474, 552, 704, 755
TscAI	CASTG	1 cut(s)	1093
TseI	GCWGC	8 cut(s)	277, 280, 290, 305, 308, 311, 397, 728
TspDTI	ATGAA	3 cut(s)	138, 578, 581
TspGWI	ACGGA	1 cut(s)	674
TspRI	CASTG	1 cut(s)	1093
Tth111I	GACNNNGTC	1 cut(s)	979
VpaK11BI	GGWCC	1 cut(s)	955
XagI	CCTNNNNNAGG	1 cut(s)	991
XapI	RAATTY	2 cut(s)	126, 620
XbaI	TCTAGA	1 cut(s)	46
XceI	RCATGY	3 cut(s)	260, 297, 518
XcmI	CCANNNNNNNNNTGG	1 cut(s)	610
XhoI	CTCGAG	1 cut(s)	149
XmiI	GTMKAC	2 cut(s)	833, 983
XspI	CTAG	3 cut(s)	47, 677, 747
Zsp2I	ATGCAT	1 cut(s)	786

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.