MD01G1232800.v1.1

Lecithin retinol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0002037
Physical Location & Seq
Forward (+)
32109443 .. 32110413
971 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1232800.v1.1.491

Sequence Viewer

Length: 642 bp
ATGAGTCTATTGTCAAACAGGATTTCCAGGGAGGAGCTCCAGCCGGGAGATCACATATATGCTTATAGAAAAGCGCATGCATACTCCCACCATGGTATATACGTGGGGGAAGGTAGGGTTATTCATTTTGTTGCAACAAAGGAAAAAACAAGAAGTACGAGAGAGCCTCGATGCAGCGACTGCAAGAACGACCCAAACACGGCACGCGGCGTGCTGAAAACCTGCGTCGATTGCTTCCTGAAAGGTCACGGCCTTCTTCGATTCGAATACAACGTGTCTCACGATCGCTTCAAATTCAAACACAAGGGGACTTGCAGCACTGAGAGTTGTGATCCGAGAGACGAAACGGTTCGTCGTGCCTCAGAGATTTTCGACAAGGATCGGACTCATGGGACGGGTTTCGGTGAATACGATTTGCTTAAAAACAACTGCGAGGACTTTGCCAGCTATTGCAAAAGAGGCACGAGCGTGAGCGAGCAGGTTGTGAGGGCTATGTCTGTGGGGAACAGTTTTGCAGATTCATCAAGTAGTAGTAGTACTAGTAGTGCTGCTTCACCATCCGGAATTGATGATGCTGATAATAATTCCAAGACTGCTTTTCTTATTTCCAAGGCTACTTCTTTCTTTTCTTCTGTGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRAT

Protein Analysis

214

Amino Acids

23.61

Weight (kDa)

8.39

Isoelectric Point (pI)

36.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRAT PF04970 12 - 156 2.4e-26 Lecithin retinol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 230, 469
AccII CGCG 1 cut(s) 207
AccIII TCCGGA 1 cut(s) 560
AciI CCGC 1 cut(s) 207
AclWI GGATC 2 cut(s) 326, 387
AcsI RAATTY 1 cut(s) 293
AfaI GTAC 2 cut(s) 157, 538
AfiI CCNNNNNNNGG 1 cut(s) 199
AflIII ACRYGT 1 cut(s) 273
AgsI TTSAA 2 cut(s) 292, 298
AhlI ACTAGT 1 cut(s) 539
AjnI CCWGG 1 cut(s) 26
AleI CACNNNNGTG 1 cut(s) 467
AluBI AGCT 2 cut(s) 37, 447
AluI AGCT 2 cut(s) 37, 447
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 2 cut(s) 282, 333
AlwI GGATC 2 cut(s) 326, 387
AlwNI CAGNNNCTG 1 cut(s) 180
Aor13HI TCCGGA 1 cut(s) 560
AoxI GGCC 1 cut(s) 250
ApeKI GCWGC 3 cut(s) 174, 315, 548
ApoI RAATTY 1 cut(s) 293
Asp700I GAANNNNTTC 1 cut(s) 348
AspLEI GCGC 1 cut(s) 76
AsuC2I CCSGG 1 cut(s) 45
AsuHPI GGTGA 2 cut(s) 416, 546
AsuII TTCGAA 1 cut(s) 264
BanII GRGCYC 1 cut(s) 39
BarI GAAGNNNNNNTAC 2 cut(s) 535, 567
BauI CACGAG 1 cut(s) 463
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 3 cut(s) 186, 327, 535
BccI CCATC 1 cut(s) 565
BceAI ACGGC 2 cut(s) 216, 265
BcgI CGANNNNNNTGC 2 cut(s) 422, 456
BciT130I CCWGG 1 cut(s) 28
BcnI CCSGG 1 cut(s) 45
BcoDI GTCTC 2 cut(s) 282, 333
BcuI ACTAGT 1 cut(s) 539
BfaI CTAG 1 cut(s) 540
BfuAI ACCTGC 2 cut(s) 230, 469
BisI GCNGC 4 cut(s) 175, 208, 316, 549
BlsI GCNGC 4 cut(s) 176, 209, 317, 550
BmcAI AGTACT 1 cut(s) 538
Bme1390I CCNGG 2 cut(s) 28, 45
BmrFI CCNGG 2 cut(s) 28, 45
BmsI GCATC 2 cut(s) 161, 562
BplI GAGNNNNNCTC 2 cut(s) 151, 183
BpmI CTGGAG 1 cut(s) 23
Bpu14I TTCGAA 1 cut(s) 264
BpuMI CCSGG 1 cut(s) 45
BsaAI YACGTR 1 cut(s) 103
BsaJI CCNNGG 3 cut(s) 27, 91, 609
BsaWI WCCGGW 1 cut(s) 560
Bsc4I CCNNNNNNNGG 1 cut(s) 199
BseAI TCCGGA 1 cut(s) 560
BseBI CCWGG 1 cut(s) 28
BseDI CCNNGG 3 cut(s) 27, 91, 609
BseGI GGATG 1 cut(s) 557
BseLI CCNNNNNNNGG 1 cut(s) 199
BseMII CTCAG 2 cut(s) 312, 375
BseRI GAGGAG 1 cut(s) 47
BseXI GCAGC 3 cut(s) 186, 327, 535
Bsh1236I CGCG 1 cut(s) 207
Bsh1285I CGRYCG 1 cut(s) 286
BshFI GGCC 1 cut(s) 252
BsiEI CGRYCG 1 cut(s) 286
BsiHKAI GWGCWC 1 cut(s) 39
BsiSI CCGG 2 cut(s) 44, 561
BslFI GGGAC 2 cut(s) 322, 406
BslI CCNNNNNNNGG 1 cut(s) 199
BsmAI GTCTC 2 cut(s) 282, 333
BsmBI CGTCTC 1 cut(s) 333
BsmFI GGGAC 2 cut(s) 322, 406
BsnI GGCC 1 cut(s) 252
Bsp119I TTCGAA 1 cut(s) 264
Bsp1286I GDGCHC 1 cut(s) 39
Bsp13I TCCGGA 1 cut(s) 560
Bsp143I GATC 4 cut(s) 49, 283, 331, 379
Bsp19I CCATGG 1 cut(s) 91
BspACI CCGC 1 cut(s) 207
BspANI GGCC 1 cut(s) 252
BspCNI CTCAG 2 cut(s) 313, 374
BspEI TCCGGA 1 cut(s) 560
BspFNI CGCG 1 cut(s) 207
BspMI ACCTGC 2 cut(s) 230, 469
BspPI GGATC 2 cut(s) 326, 387
BspT104I TTCGAA 1 cut(s) 264
BssECI CCNNGG 3 cut(s) 27, 91, 609
BssMI GATC 4 cut(s) 49, 283, 331, 379
BssSI CACGAG 1 cut(s) 463
BssT1I CCWWGG 2 cut(s) 91, 609
Bst2BI CACGAG 1 cut(s) 463
Bst2UI CCWGG 1 cut(s) 28
Bst4CI ACNGT 2 cut(s) 349, 509
BstAPI GCANNNNNTGC 1 cut(s) 180
BstBAI YACGTR 1 cut(s) 103
BstBI TTCGAA 1 cut(s) 264
BstC8I GCNNGC 5 cut(s) 78, 205, 212, 445, 476
BstDEI CTNAG 2 cut(s) 321, 361
BstDSI CCRYGG 1 cut(s) 91
BstF5I GGATG 1 cut(s) 557
BstFNI CGCG 1 cut(s) 207
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 4 cut(s) 52, 286, 334, 382
BstMAI GTCTC 2 cut(s) 282, 333
BstMBI GATC 4 cut(s) 49, 283, 331, 379
BstMCI CGRYCG 1 cut(s) 286
BstMWI GCNNNNNNNGC 3 cut(s) 180, 231, 459
BstNI CCWGG 1 cut(s) 28
BstNSI RCATGY 1 cut(s) 80
BstSCI CCNGG 2 cut(s) 26, 43
BstUI CGCG 1 cut(s) 207
BstV1I GCAGC 3 cut(s) 186, 327, 535
BsuRI GGCC 1 cut(s) 252
BtgI CCRYGG 1 cut(s) 91
BtsCI GGATG 1 cut(s) 557
BtsIMutI CAGTG 1 cut(s) 318
BveI ACCTGC 2 cut(s) 230, 469
Cac8I GCNNGC 5 cut(s) 78, 205, 212, 445, 476
CaiI CAGNNNCTG 1 cut(s) 180
CfoI GCGC 1 cut(s) 76
CseI GACGC 1 cut(s) 214
Csp6I GTAC 2 cut(s) 156, 537
CviAII CATG 3 cut(s) 77, 92, 389
CviJI RGCY 7 cut(s) 37, 43, 166, 252, 447, 491, 614
CviKI_1 RGCY 7 cut(s) 37, 43, 166, 252, 447, 491, 614
CviQI GTAC 2 cut(s) 156, 537
DdeI CTNAG 2 cut(s) 321, 361
DpnI GATC 4 cut(s) 51, 285, 333, 381
DpnII GATC 4 cut(s) 49, 283, 331, 379
Ecl136II GAGCTC 1 cut(s) 37
Eco130I CCWWGG 2 cut(s) 91, 609
Eco24I GRGCYC 1 cut(s) 39
Eco53kI GAGCTC 1 cut(s) 37
EcoICRI GAGCTC 1 cut(s) 37
EcoRII CCWGG 1 cut(s) 26
EcoT14I CCWWGG 2 cut(s) 91, 609
EcoT22I ATGCAT 1 cut(s) 82
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 2 cut(s) 91, 609
Esp3I CGTCTC 1 cut(s) 333
FaeI CATG 3 cut(s) 80, 95, 392
FaqI GGGAC 2 cut(s) 322, 406
FatI CATG 3 cut(s) 76, 91, 388
Fnu4HI GCNGC 4 cut(s) 175, 208, 316, 549
FokI GGATG 1 cut(s) 544
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 4 cut(s) 175, 208, 316, 549
FspBI CTAG 1 cut(s) 540
GlaI GCGC 1 cut(s) 75
GluI GCNGC 4 cut(s) 175, 208, 316, 549
GsuI CTGGAG 1 cut(s) 23
HaeIII GGCC 1 cut(s) 252
HapII CCGG 2 cut(s) 44, 561
HgaI GACGC 1 cut(s) 214
HhaI GCGC 1 cut(s) 76
Hin1II CATG 3 cut(s) 80, 95, 392
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HinfI GANTC 4 cut(s) 4, 261, 385, 518
HpaII CCGG 2 cut(s) 44, 561
HphI GGTGA 2 cut(s) 416, 546
Hpy188I TCNGA 3 cut(s) 336, 364, 384
Hpy188III TCNNGA 3 cut(s) 238, 281, 561
Hpy99I CGWCG 2 cut(s) 230, 357
HpyAV CCTTC 2 cut(s) 104, 263
HpyCH4III ACNGT 2 cut(s) 349, 509
HpyCH4IV ACGT 2 cut(s) 102, 273
HpyCH4V TGCA 7 cut(s) 80, 134, 174, 183, 315, 453, 515
HpyF10VI GCNNNNNNNGC 3 cut(s) 180, 231, 459
HpyF3I CTNAG 2 cut(s) 321, 361
HpySE526I ACGT 2 cut(s) 102, 273
Hsp92II CATG 3 cut(s) 80, 95, 392
HspAI GCGC 1 cut(s) 74
Kpn2I TCCGGA 1 cut(s) 560
Kzo9I GATC 4 cut(s) 49, 283, 331, 379
LmnI GCTCC 2 cut(s) 34, 42
Lsp1109I GCAGC 3 cut(s) 186, 327, 535
LweI GCATC 2 cut(s) 161, 562
MaeI CTAG 1 cut(s) 540
MaeII ACGT 2 cut(s) 102, 273
MaeIII GTNAC 1 cut(s) 245
MalI GATC 4 cut(s) 51, 285, 333, 381
MboI GATC 4 cut(s) 49, 283, 331, 379
MboII GAAGA 2 cut(s) 248, 621
MhlI GDGCHC 1 cut(s) 39
MluCI AATT 3 cut(s) 293, 564, 583
MlyI GAGTC 2 cut(s) 13, 379
MnlI CCTC 6 cut(s) 25, 177, 370, 427, 452, 480
Mph1103I ATGCAT 1 cut(s) 82
MroI TCCGGA 1 cut(s) 560
MroXI GAANNNNTTC 1 cut(s) 348
MseI TTAA 1 cut(s) 420
MslI CAYNNNNRTG 2 cut(s) 57, 467
MspI CCGG 2 cut(s) 44, 561
MspR9I CCNGG 2 cut(s) 28, 45
MvaI CCWGG 1 cut(s) 28
MvnI CGCG 1 cut(s) 207
MwoI GCNNNNNNNGC 3 cut(s) 180, 231, 459
NciI CCSGG 1 cut(s) 45
NcoI CCATGG 1 cut(s) 91
NdeII GATC 4 cut(s) 49, 283, 331, 379
NlaIII CATG 3 cut(s) 80, 95, 392
NmuCI GTSAC 1 cut(s) 245
NsiI ATGCAT 1 cut(s) 82
NspI RCATGY 1 cut(s) 80
NspV TTCGAA 1 cut(s) 264
OliI CACNNNNGTG 1 cut(s) 467
PaeI GCATGC 1 cut(s) 80
PcsI WCGNNNNNNNCGW 3 cut(s) 270, 279, 408
PdmI GAANNNNTTC 1 cut(s) 348
PfeI GAWTC 2 cut(s) 261, 518
PkrI GCNGC 4 cut(s) 176, 209, 317, 550
Ple19I CGATCG 1 cut(s) 286
PleI GAGTC 2 cut(s) 12, 379
PpsI GAGTC 2 cut(s) 12, 379
Ppu21I YACGTR 1 cut(s) 103
Psp124BI GAGCTC 1 cut(s) 39
Psp6I CCWGG 1 cut(s) 26
PspGI CCWGG 1 cut(s) 26
PstNI CAGNNNCTG 1 cut(s) 180
PvuI CGATCG 1 cut(s) 286
RsaI GTAC 2 cut(s) 157, 538
RsaNI GTAC 2 cut(s) 156, 537
RseI CAYNNNNRTG 2 cut(s) 57, 467
SacI GAGCTC 1 cut(s) 39
SaqAI TTAA 1 cut(s) 420
SatI GCNGC 4 cut(s) 175, 208, 316, 549
Sau3AI GATC 4 cut(s) 49, 283, 331, 379
ScaI AGTACT 1 cut(s) 538
SchI GAGTC 2 cut(s) 13, 379
ScrFI CCNGG 2 cut(s) 28, 45
SduI GDGCHC 1 cut(s) 39
SetI ASST 8 cut(s) 39, 105, 115, 224, 247, 276, 449, 483
SfaNI GCATC 2 cut(s) 161, 562
SfuI TTCGAA 1 cut(s) 264
SmiMI CAYNNNNRTG 2 cut(s) 57, 467
SpeI ACTAGT 1 cut(s) 539
SphI GCATGC 1 cut(s) 80
Sse9I AATT 3 cut(s) 293, 564, 583
SsiI CCGC 1 cut(s) 207
SspMI CTAG 1 cut(s) 540
SstI GAGCTC 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 26, 43
StyI CCWWGG 2 cut(s) 91, 609
TaaI ACNGT 2 cut(s) 349, 509
TaiI ACGT 2 cut(s) 105, 276
TaqI TCGA 5 cut(s) 169, 228, 259, 264, 372
TasI AATT 3 cut(s) 293, 564, 583
TatI WGTACW 1 cut(s) 536
TauI GCSGC 1 cut(s) 210
TfiI GAWTC 2 cut(s) 261, 518
Tru1I TTAA 1 cut(s) 420
Tru9I TTAA 1 cut(s) 420
TscAI CASTG 1 cut(s) 325
TseFI GTSAC 1 cut(s) 245
TseI GCWGC 3 cut(s) 174, 315, 548
Tsp45I GTSAC 1 cut(s) 245
TspDTI ATGAA 2 cut(s) 113, 510
TspRI CASTG 1 cut(s) 325
XapI RAATTY 1 cut(s) 293
XceI RCATGY 1 cut(s) 80
XmnI GAANNNNTTC 1 cut(s) 348
XspI CTAG 1 cut(s) 540
ZrmI AGTACT 1 cut(s) 538
Zsp2I ATGCAT 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.