pycom01g24120

Lecithin retinol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0002037
Physical Location & Seq
Forward (+)
21158839 .. 21159673
835 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g24120.2

Sequence Viewer

Length: 645 bp
ATGGGTCTATTGTCAAACAGGATTTCCAGGGAGGAGCTCCAGCCGGGAGATCACATTTATGCTTATAGAAAAGCGCATGCATACTCCCACCACGGCATATACGTGGGGGAAGGTAGGGTTATTCATTTCGTTAAAACAAAGGGAAAAGGGAAAACAAGTAGTAAGAGAAAGCCTCCATGCAGTGACTGCAAGAACGACCCAAACACGGCACGCGGAGTGCTAAAAACCTGCGTCGATTGCTTCCTGAAAGGTCACGGCCTTTTTCGATTCGAATACAACGTGTCTCACGATCGCTTCAATATAAAAAACAAGGGGACTTGCAGCACTGAGAGTTGTTATCCGGGAGGCGAAACGGTTCGTCGTGCCTCAGAGATTTTCGACAAGGATCCGAATCATGGGACGGGTTTCGGTGAATATGATTTGCTTAAAAACAACTGCGAGGACTTTGCCAGCTATTGCAAAAGAGGCACGAACGTGAGCGAGCAGGTTGTGAGGGTTTCGTCTGTGGGGAATAGTTTTGCAGATTCATCAAATAGTAGTTGTACTAGTAGTGCTGCTTCTCCAACCGGAATTGATGATGCTGCTACTTCCAAGACTGCTTTTCTTATTTCCAAGGCTACTTCTTTCTTTTCTTCTGTGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRAT

Protein Analysis

215

Amino Acids

23.35

Weight (kDa)

8.92

Isoelectric Point (pI)

32.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 236, 475
AccII CGCG 1 cut(s) 213
AciI CCGC 1 cut(s) 213
AclWI GGATC 2 cut(s) 380, 393
AfaI GTAC 1 cut(s) 544
AfiI CCNNNNNNNGG 2 cut(s) 205, 395
AflIII ACRYGT 1 cut(s) 279
AgsI TTSAA 1 cut(s) 298
AhlI ACTAGT 1 cut(s) 545
AjnI CCWGG 1 cut(s) 26
AleI CACNNNNGTG 1 cut(s) 473
AluBI AGCT 2 cut(s) 37, 453
AluI AGCT 2 cut(s) 37, 453
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 1 cut(s) 288
AlwI GGATC 2 cut(s) 380, 393
AlwNI CAGNNNCTG 1 cut(s) 186
AoxI GGCC 1 cut(s) 256
ApeKI GCWGC 3 cut(s) 321, 554, 581
Asp700I GAANNNNTTC 1 cut(s) 354
AspLEI GCGC 1 cut(s) 76
AsuC2I CCSGG 2 cut(s) 45, 342
AsuHPI GGTGA 1 cut(s) 422
AsuII TTCGAA 1 cut(s) 270
BamHI GGATCC 1 cut(s) 385
BanII GRGCYC 1 cut(s) 39
BarI GAAGNNNNNNTAC 2 cut(s) 541, 573
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 3 cut(s) 333, 541, 568
BceAI ACGGC 3 cut(s) 109, 222, 271
BcgI CGANNNNNNTGC 2 cut(s) 428, 462
BciT130I CCWGG 1 cut(s) 28
BcnI CCSGG 2 cut(s) 45, 342
BcoDI GTCTC 1 cut(s) 288
BcuI ACTAGT 1 cut(s) 545
BfaI CTAG 1 cut(s) 546
BfuAI ACCTGC 2 cut(s) 236, 475
BisI GCNGC 3 cut(s) 322, 555, 582
BlsI GCNGC 3 cut(s) 323, 556, 583
Bme1390I CCNGG 3 cut(s) 28, 45, 342
BmiI GGNNCC 1 cut(s) 387
BmrFI CCNGG 3 cut(s) 28, 45, 342
BmsI GCATC 1 cut(s) 568
BplI GAGNNNNNCTC 2 cut(s) 157, 189
BpmI CTGGAG 1 cut(s) 23
Bpu14I TTCGAA 1 cut(s) 270
BpuMI CCSGG 2 cut(s) 45, 342
BsaAI YACGTR 1 cut(s) 103
BsaBI GATNNNNATC 1 cut(s) 390
BsaJI CCNNGG 3 cut(s) 27, 91, 612
BsaWI WCCGGW 1 cut(s) 566
Bsc4I CCNNNNNNNGG 2 cut(s) 205, 395
Bse8I GATNNNNATC 1 cut(s) 390
BseBI CCWGG 1 cut(s) 28
BseDI CCNNGG 3 cut(s) 27, 91, 612
BseJI GATNNNNATC 1 cut(s) 390
BseLI CCNNNNNNNGG 2 cut(s) 205, 395
BseMII CTCAG 2 cut(s) 318, 381
BseRI GAGGAG 1 cut(s) 47
BseXI GCAGC 3 cut(s) 333, 541, 568
Bsh1236I CGCG 1 cut(s) 213
Bsh1285I CGRYCG 1 cut(s) 292
BshFI GGCC 1 cut(s) 258
BsiEI CGRYCG 1 cut(s) 292
BsiHKAI GWGCWC 1 cut(s) 39
BsiSI CCGG 3 cut(s) 44, 341, 567
BslFI GGGAC 2 cut(s) 328, 412
BslI CCNNNNNNNGG 2 cut(s) 205, 395
BsmAI GTCTC 1 cut(s) 288
BsmFI GGGAC 2 cut(s) 328, 412
BsnI GGCC 1 cut(s) 258
Bsp119I TTCGAA 1 cut(s) 270
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 3 cut(s) 49, 289, 385
BspACI CCGC 1 cut(s) 213
BspANI GGCC 1 cut(s) 258
BspCNI CTCAG 2 cut(s) 319, 380
BspFNI CGCG 1 cut(s) 213
BspLI GGNNCC 1 cut(s) 387
BspMI ACCTGC 2 cut(s) 236, 475
BspPI GGATC 2 cut(s) 380, 393
BspT104I TTCGAA 1 cut(s) 270
BssECI CCNNGG 3 cut(s) 27, 91, 612
BssMI GATC 3 cut(s) 49, 289, 385
BssT1I CCWWGG 1 cut(s) 612
Bst2UI CCWGG 1 cut(s) 28
Bst4CI ACNGT 1 cut(s) 355
BstAPI GCANNNNNTGC 1 cut(s) 186
BstBAI YACGTR 1 cut(s) 103
BstBI TTCGAA 1 cut(s) 270
BstC8I GCNNGC 4 cut(s) 78, 211, 451, 482
BstDEI CTNAG 2 cut(s) 327, 367
BstDSI CCRYGG 1 cut(s) 91
BstFNI CGCG 1 cut(s) 213
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 3 cut(s) 52, 292, 388
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 3 cut(s) 49, 289, 385
BstMCI CGRYCG 1 cut(s) 292
BstMWI GCNNNNNNNGC 3 cut(s) 186, 237, 465
BstNI CCWGG 1 cut(s) 28
BstNSI RCATGY 1 cut(s) 80
BstSCI CCNGG 3 cut(s) 26, 43, 340
BstUI CGCG 1 cut(s) 213
BstV1I GCAGC 3 cut(s) 333, 541, 568
BstX2I RGATCY 1 cut(s) 385
BstYI RGATCY 1 cut(s) 385
BsuRI GGCC 1 cut(s) 258
BtgI CCRYGG 1 cut(s) 91
BtsI GCAGTG 1 cut(s) 187
BtsIMutI CAGTG 2 cut(s) 187, 324
BveI ACCTGC 2 cut(s) 236, 475
Cac8I GCNNGC 4 cut(s) 78, 211, 451, 482
CaiI CAGNNNCTG 1 cut(s) 186
CfoI GCGC 1 cut(s) 76
CseI GACGC 1 cut(s) 220
Csp6I GTAC 1 cut(s) 543
CviAII CATG 3 cut(s) 77, 177, 395
CviJI RGCY 6 cut(s) 37, 43, 172, 258, 453, 617
CviKI_1 RGCY 6 cut(s) 37, 43, 172, 258, 453, 617
CviQI GTAC 1 cut(s) 543
DdeI CTNAG 2 cut(s) 327, 367
DpnI GATC 3 cut(s) 51, 291, 387
DpnII GATC 3 cut(s) 49, 289, 385
Ecl136II GAGCTC 1 cut(s) 37
Eco130I CCWWGG 1 cut(s) 612
Eco24I GRGCYC 1 cut(s) 39
Eco53kI GAGCTC 1 cut(s) 37
EcoICRI GAGCTC 1 cut(s) 37
EcoRII CCWGG 1 cut(s) 26
EcoT14I CCWWGG 1 cut(s) 612
EcoT22I ATGCAT 1 cut(s) 82
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 612
FaeI CATG 3 cut(s) 80, 180, 398
FaqI GGGAC 2 cut(s) 328, 412
FatI CATG 3 cut(s) 76, 176, 394
Fnu4HI GCNGC 3 cut(s) 322, 555, 582
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 3 cut(s) 322, 555, 582
FspBI CTAG 1 cut(s) 546
GlaI GCGC 1 cut(s) 75
GluI GCNGC 3 cut(s) 322, 555, 582
GsuI CTGGAG 1 cut(s) 23
HaeIII GGCC 1 cut(s) 258
HapII CCGG 3 cut(s) 44, 341, 567
HgaI GACGC 1 cut(s) 220
HhaI GCGC 1 cut(s) 76
Hin1II CATG 3 cut(s) 80, 180, 398
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HinfI GANTC 3 cut(s) 267, 391, 524
HpaII CCGG 3 cut(s) 44, 341, 567
HphI GGTGA 1 cut(s) 422
Hpy188I TCNGA 2 cut(s) 370, 390
Hpy188III TCNNGA 3 cut(s) 244, 287, 642
Hpy99I CGWCG 2 cut(s) 236, 363
HpyAV CCTTC 1 cut(s) 104
HpyCH4III ACNGT 1 cut(s) 355
HpyCH4IV ACGT 3 cut(s) 102, 279, 474
HpyCH4V TGCA 6 cut(s) 80, 180, 189, 321, 459, 521
HpyF10VI GCNNNNNNNGC 3 cut(s) 186, 237, 465
HpyF3I CTNAG 2 cut(s) 327, 367
HpySE526I ACGT 3 cut(s) 102, 279, 474
Hsp92II CATG 3 cut(s) 80, 180, 398
HspAI GCGC 1 cut(s) 74
Kzo9I GATC 3 cut(s) 49, 289, 385
LmnI GCTCC 2 cut(s) 34, 42
Lsp1109I GCAGC 3 cut(s) 333, 541, 568
LweI GCATC 1 cut(s) 568
MaeI CTAG 1 cut(s) 546
MaeII ACGT 3 cut(s) 102, 279, 474
MaeIII GTNAC 2 cut(s) 182, 251
MalI GATC 3 cut(s) 51, 291, 387
MboI GATC 3 cut(s) 49, 289, 385
MboII GAAGA 1 cut(s) 624
MflI RGATCY 1 cut(s) 385
MhlI GDGCHC 1 cut(s) 39
MluCI AATT 1 cut(s) 570
MmeI TCCRAC 1 cut(s) 587
MnlI CCTC 7 cut(s) 25, 183, 338, 376, 433, 458, 486
Mph1103I ATGCAT 1 cut(s) 82
MroXI GAANNNNTTC 1 cut(s) 354
MseI TTAA 2 cut(s) 132, 426
MslI CAYNNNNRTG 3 cut(s) 57, 101, 473
MspI CCGG 3 cut(s) 44, 341, 567
MspR9I CCNGG 3 cut(s) 28, 45, 342
MvaI CCWGG 1 cut(s) 28
MvnI CGCG 1 cut(s) 213
MwoI GCNNNNNNNGC 3 cut(s) 186, 237, 465
NciI CCSGG 2 cut(s) 45, 342
NdeII GATC 3 cut(s) 49, 289, 385
NlaIII CATG 3 cut(s) 80, 180, 398
NlaIV GGNNCC 1 cut(s) 387
NmuCI GTSAC 2 cut(s) 182, 251
NsiI ATGCAT 1 cut(s) 82
NspI RCATGY 1 cut(s) 80
NspV TTCGAA 1 cut(s) 270
OliI CACNNNNGTG 1 cut(s) 473
PaeI GCATGC 1 cut(s) 80
PcsI WCGNNNNNNNCGW 3 cut(s) 99, 276, 285
PdmI GAANNNNTTC 1 cut(s) 354
PfeI GAWTC 3 cut(s) 267, 391, 524
PfoI TCCNGGA 1 cut(s) 340
PkrI GCNGC 3 cut(s) 323, 556, 583
Ple19I CGATCG 1 cut(s) 292
Ppu21I YACGTR 1 cut(s) 103
Psp124BI GAGCTC 1 cut(s) 39
Psp6I CCWGG 1 cut(s) 26
PspGI CCWGG 1 cut(s) 26
PspN4I GGNNCC 1 cut(s) 387
PstNI CAGNNNCTG 1 cut(s) 186
PsuI RGATCY 1 cut(s) 385
PvuI CGATCG 1 cut(s) 292
RsaI GTAC 1 cut(s) 544
RsaNI GTAC 1 cut(s) 543
RseI CAYNNNNRTG 3 cut(s) 57, 101, 473
SacI GAGCTC 1 cut(s) 39
SaqAI TTAA 2 cut(s) 132, 426
SatI GCNGC 3 cut(s) 322, 555, 582
Sau3AI GATC 3 cut(s) 49, 289, 385
ScrFI CCNGG 3 cut(s) 28, 45, 342
SduI GDGCHC 1 cut(s) 39
SetI ASST 9 cut(s) 39, 105, 115, 230, 253, 282, 455, 477, 489
SfaNI GCATC 1 cut(s) 568
SfuI TTCGAA 1 cut(s) 270
SmiMI CAYNNNNRTG 3 cut(s) 57, 101, 473
SpeI ACTAGT 1 cut(s) 545
SphI GCATGC 1 cut(s) 80
Sse9I AATT 1 cut(s) 570
SsiI CCGC 1 cut(s) 213
SspMI CTAG 1 cut(s) 546
SstI GAGCTC 1 cut(s) 39
StyD4I CCNGG 3 cut(s) 26, 43, 340
StyI CCWWGG 1 cut(s) 612
TaaI ACNGT 1 cut(s) 355
TaiI ACGT 3 cut(s) 105, 282, 477
TaqI TCGA 4 cut(s) 234, 265, 270, 378
TasI AATT 1 cut(s) 570
TatI WGTACW 1 cut(s) 542
TfiI GAWTC 3 cut(s) 267, 391, 524
Tru1I TTAA 2 cut(s) 132, 426
Tru9I TTAA 2 cut(s) 132, 426
TscAI CASTG 2 cut(s) 187, 331
TseFI GTSAC 2 cut(s) 182, 251
TseI GCWGC 3 cut(s) 321, 554, 581
Tsp45I GTSAC 2 cut(s) 182, 251
TspDTI ATGAA 2 cut(s) 113, 516
TspRI CASTG 2 cut(s) 187, 331
XceI RCATGY 1 cut(s) 80
XmnI GAANNNNTTC 1 cut(s) 354
XspI CTAG 1 cut(s) 546
Zsp2I ATGCAT 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.