MD07G1305200.v1.1

Lecithin retinol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002037
Physical Location & Seq
Forward (+)
36223101 .. 36223972
872 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1305200.v1.1.491

Sequence Viewer

Length: 468 bp
ATGGGACTGTTCTCAAACAAGGTTGATAAGTCCCAGATCAAGCCAGGAGATCACGTCTATACCTACAGAGCACTGCACACATACTCCCACCATGGAATATGCGTGGAGGCAGATAGGGTGATCCACTACACCAGAACACAAGTTGCAAAAAAGAAATTATGGATGTTTTGGATTACTAAATGCAAAAACTGCGGGCATCACCCAGATATGGCACGAGGAGTGATCAAAACTTGCGTCAACTGCTTCCTAGACGGCCATGGGCTTTACCGGTTCGAATACGGAGTCTCTCAGGCTCACTTCCTATTGAAATTTCCAGGAACTTGCACGACGGGTCGTTCCAATTCAGCTGACAAAGTCATACCTCGCGCCACGGAATTGCTCAACAAGGAACAGGGTTTCGGGGACTATAACCTGTTTGAAAACAACTGCGAATCGTTTGCGATTTTCTGCAAAACAGGAAAACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRAT

Protein Analysis

156

Amino Acids

17.68

Weight (kDa)

9.12

Isoelectric Point (pI)

10.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRAT PF04970 12 - 154 2.5e-24 Lecithin retinol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 366
AciI CCGC 1 cut(s) 192
AclWI GGATC 1 cut(s) 115
AcoI YGGCCR 1 cut(s) 253
AcsI RAATTY 1 cut(s) 308
AfiI CCNNNNNNNGG 1 cut(s) 208
AgeI ACCGGT 1 cut(s) 267
AgsI TTSAA 2 cut(s) 307, 419
AjiI CACGTC 1 cut(s) 55
AjnI CCWGG 2 cut(s) 43, 313
AluBI AGCT 1 cut(s) 347
AluI AGCT 1 cut(s) 347
Alw21I GWGCWC 1 cut(s) 73
Alw26I GTCTC 1 cut(s) 289
AlwI GGATC 1 cut(s) 115
AoxI GGCC 1 cut(s) 253
ApoI RAATTY 1 cut(s) 308
ArsI GACNNNNNNTTYG 4 cut(s) 219, 251, 267, 299
AsiGI ACCGGT 1 cut(s) 267
AspLEI GCGC 1 cut(s) 368
AsuHPI GGTGA 2 cut(s) 130, 191
AsuII TTCGAA 1 cut(s) 273
BauI CACGAG 1 cut(s) 213
Bbv12I GWGCWC 1 cut(s) 73
BceAI ACGGC 1 cut(s) 268
BcgI CGANNNNNNTGC 2 cut(s) 419, 453
BciT130I CCWGG 2 cut(s) 45, 315
BclI TGATCA 1 cut(s) 222
BcoDI GTCTC 1 cut(s) 289
BfaI CTAG 1 cut(s) 248
BfmI CTRYAG 1 cut(s) 64
Bme1390I CCNGG 2 cut(s) 45, 315
BmgBI CACGTC 1 cut(s) 55
BmrFI CCNGG 2 cut(s) 45, 315
BmsI GCATC 1 cut(s) 205
Bpu14I TTCGAA 1 cut(s) 273
BsaJI CCNNGG 3 cut(s) 91, 256, 369
BsaWI WCCGGW 1 cut(s) 267
BsaXI ACNNNNNCTCC 4 cut(s) 39, 68, 69, 98
Bsc4I CCNNNNNNNGG 1 cut(s) 208
Bse118I RCCGGY 1 cut(s) 267
BseBI CCWGG 2 cut(s) 45, 315
BseDI CCNNGG 3 cut(s) 91, 256, 369
BseGI GGATG 1 cut(s) 168
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMII CTCAG 1 cut(s) 302
BseRI GAGGAG 1 cut(s) 231
BsgI GTGCAG 1 cut(s) 59
Bsh1236I CGCG 1 cut(s) 366
BshFI GGCC 1 cut(s) 255
BshTI ACCGGT 1 cut(s) 267
BsiHKAI GWGCWC 1 cut(s) 73
BsiSI CCGG 1 cut(s) 268
BslFI GGGAC 3 cut(s) 16, 18, 416
BslI CCNNNNNNNGG 1 cut(s) 208
BsmAI GTCTC 1 cut(s) 289
BsmFI GGGAC 3 cut(s) 16, 18, 416
BsnI GGCC 1 cut(s) 255
Bsp119I TTCGAA 1 cut(s) 273
Bsp1286I GDGCHC 1 cut(s) 73
Bsp143I GATC 4 cut(s) 36, 49, 120, 222
Bsp19I CCATGG 2 cut(s) 91, 256
BspACI CCGC 1 cut(s) 192
BspANI GGCC 1 cut(s) 255
BspCNI CTCAG 1 cut(s) 301
BspFNI CGCG 1 cut(s) 366
BspPI GGATC 1 cut(s) 115
BspT104I TTCGAA 1 cut(s) 273
BsrFI RCCGGY 1 cut(s) 267
BssAI RCCGGY 1 cut(s) 267
BssECI CCNNGG 3 cut(s) 91, 256, 369
BssMI GATC 4 cut(s) 36, 49, 120, 222
BssSI CACGAG 1 cut(s) 213
BssT1I CCWWGG 2 cut(s) 91, 256
Bst2BI CACGAG 1 cut(s) 213
Bst2UI CCWGG 2 cut(s) 45, 315
Bst4CI ACNGT 1 cut(s) 9
BstAPI GCANNNNNTGC 1 cut(s) 189
BstBI TTCGAA 1 cut(s) 273
BstC8I GCNNGC 1 cut(s) 194
BstDEI CTNAG 1 cut(s) 288
BstDSI CCRYGG 3 cut(s) 91, 256, 369
BstF5I GGATG 1 cut(s) 168
BstFNI CGCG 1 cut(s) 366
BstHHI GCGC 1 cut(s) 368
BstKTI GATC 4 cut(s) 39, 52, 123, 225
BstMAI GTCTC 1 cut(s) 289
BstMBI GATC 4 cut(s) 36, 49, 120, 222
BstMWI GCNNNNNNNGC 2 cut(s) 189, 240
BstNI CCWGG 2 cut(s) 45, 315
BstSCI CCNGG 2 cut(s) 43, 313
BstSFI CTRYAG 1 cut(s) 64
BstUI CGCG 1 cut(s) 366
BsuRI GGCC 1 cut(s) 255
BtgI CCRYGG 3 cut(s) 91, 256, 369
BtrI CACGTC 1 cut(s) 55
BtsCI GGATG 1 cut(s) 168
BtsI GCAGTG 1 cut(s) 71
BtsIMutI CAGTG 1 cut(s) 71
Cac8I GCNNGC 1 cut(s) 194
CfoI GCGC 1 cut(s) 368
Cfr10I RCCGGY 1 cut(s) 267
CseI GACGC 1 cut(s) 223
CspAI ACCGGT 1 cut(s) 267
CviAII CATG 2 cut(s) 92, 257
CviJI RGCY 5 cut(s) 43, 255, 262, 293, 347
CviKI_1 RGCY 5 cut(s) 43, 255, 262, 293, 347
DdeI CTNAG 1 cut(s) 288
DpnI GATC 4 cut(s) 38, 51, 122, 224
DpnII GATC 4 cut(s) 36, 49, 120, 222
EaeI YGGCCR 1 cut(s) 253
Eco130I CCWWGG 2 cut(s) 91, 256
EcoRII CCWGG 2 cut(s) 43, 313
EcoT14I CCWWGG 2 cut(s) 91, 256
ErhI CCWWGG 2 cut(s) 91, 256
FaeI CATG 2 cut(s) 95, 260
FaqI GGGAC 3 cut(s) 16, 18, 416
FatI CATG 2 cut(s) 91, 256
FauI CCCGC 1 cut(s) 185
FbaI TGATCA 1 cut(s) 222
FokI GGATG 1 cut(s) 175
FspBI CTAG 1 cut(s) 248
GlaI GCGC 1 cut(s) 367
HaeIII GGCC 1 cut(s) 255
HapII CCGG 1 cut(s) 268
HgaI GACGC 1 cut(s) 223
HhaI GCGC 1 cut(s) 368
Hin1II CATG 2 cut(s) 95, 260
Hin6I GCGC 1 cut(s) 366
HinP1I GCGC 1 cut(s) 366
HincII GTYRAC 1 cut(s) 238
HindII GTYRAC 1 cut(s) 238
HinfI GANTC 2 cut(s) 282, 431
HpaII CCGG 1 cut(s) 268
HphI GGTGA 2 cut(s) 130, 191
Hpy166II GTNNAC 1 cut(s) 238
Hpy8I GTNNAC 1 cut(s) 238
Hpy99I CGWCG 1 cut(s) 331
HpyCH4III ACNGT 1 cut(s) 9
HpyCH4IV ACGT 1 cut(s) 54
HpyCH4V TGCA 5 cut(s) 76, 146, 183, 324, 450
HpyF10VI GCNNNNNNNGC 2 cut(s) 189, 240
HpyF3I CTNAG 1 cut(s) 288
HpySE526I ACGT 1 cut(s) 54
Hsp92II CATG 2 cut(s) 95, 260
HspAI GCGC 1 cut(s) 366
Ksp22I TGATCA 1 cut(s) 222
Kzo9I GATC 4 cut(s) 36, 49, 120, 222
LweI GCATC 1 cut(s) 205
MaeI CTAG 1 cut(s) 248
MaeII ACGT 1 cut(s) 54
MalI GATC 4 cut(s) 38, 51, 122, 224
MboI GATC 4 cut(s) 36, 49, 120, 222
MhlI GDGCHC 1 cut(s) 73
MluCI AATT 4 cut(s) 155, 308, 340, 374
MlyI GAGTC 1 cut(s) 291
MnlI CCTC 3 cut(s) 100, 209, 372
MspA1I CMGCKG 1 cut(s) 347
MspI CCGG 1 cut(s) 268
MspR9I CCNGG 2 cut(s) 45, 315
MvaI CCWGG 2 cut(s) 45, 315
MvnI CGCG 1 cut(s) 366
MwoI GCNNNNNNNGC 2 cut(s) 189, 240
NcoI CCATGG 2 cut(s) 91, 256
NdeII GATC 4 cut(s) 36, 49, 120, 222
NlaIII CATG 2 cut(s) 95, 260
NspV TTCGAA 1 cut(s) 273
PfeI GAWTC 1 cut(s) 431
PflFI GACNNNGTC 1 cut(s) 353
PfoI TCCNGGA 1 cut(s) 313
PinAI ACCGGT 1 cut(s) 267
PleI GAGTC 1 cut(s) 290
PpsI GAGTC 1 cut(s) 290
Psp6I CCWGG 2 cut(s) 43, 313
PspGI CCWGG 2 cut(s) 43, 313
PsyI GACNNNGTC 1 cut(s) 353
PvuII CAGCTG 1 cut(s) 347
Sau3AI GATC 4 cut(s) 36, 49, 120, 222
SchI GAGTC 1 cut(s) 291
ScrFI CCNGG 2 cut(s) 45, 315
SduI GDGCHC 1 cut(s) 73
SetI ASST 6 cut(s) 24, 57, 65, 349, 364, 414
SfaNI GCATC 1 cut(s) 205
SfcI CTRYAG 1 cut(s) 64
SfuI TTCGAA 1 cut(s) 273
Sse9I AATT 4 cut(s) 155, 308, 340, 374
SsiI CCGC 1 cut(s) 192
SspMI CTAG 1 cut(s) 248
StyD4I CCNGG 2 cut(s) 43, 313
StyI CCWWGG 2 cut(s) 91, 256
TaaI ACNGT 1 cut(s) 9
TaiI ACGT 1 cut(s) 57
TaqI TCGA 1 cut(s) 273
TasI AATT 4 cut(s) 155, 308, 340, 374
TfiI GAWTC 1 cut(s) 431
TscAI CASTG 1 cut(s) 78
TspGWI ACGGA 2 cut(s) 294, 386
TspRI CASTG 1 cut(s) 78
Tth111I GACNNNGTC 1 cut(s) 353
XapI RAATTY 1 cut(s) 308
XspI CTAG 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.