Rroxscaffold_4G00277270

NC domain-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002037
Physical Location & Seq
Reverse (-)
750981 .. 751949
969 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00277270.1

Sequence Viewer

Length: 747 bp
ATGCAGCGTACGCGTTTTATAAACAAGATCGACAAAGAGAGCCTCGAGCGTGGAGATCATATTTATGCTTACAGAAAGGGTGGCTTGTACTCCCACCATGGTATCTTCATTGGGGAAGATAGGGTGATTCACTTCGTCAGAACAAATGAGGGAAACACATCGAGACGAGTCGAGCCATGCCAAAAGTGCGGGGTTGACAAAAATCACCTCCGAGGAGTCGTCAAGTCCTGCGTTGACTGCTTCCTCAATGGCCACAGCCTCCGCCGCTACCGATACAGTGTAAGTCATCGCCGCTTTGCTACCAACCTTGGAGGCACCTGCACCACCGGCGATGCTGACCCGCCAGATGTGACCATCAGCCGCGCCGAAGACCTGTTTAACACGAAAGATTTTGGGGACTATGACTTGCTTAAGAACAACTGCGAGACCTTTGCGGTGTTCTGCAAGACGGGGAAGCGCAGGAGCGACCAGGCGGACACGGGTATATTGCTGATCAAGGCGGCGGTTGAGGTGGCGATTAAGAGGCTTTATCATGATGTGGAGACTCATCACGGCGAGGACAAGATCAAAGAGTTAAAGCAGGCGATTGACAGGTTTTTTAGTGGTCTAGATCTGTCGATTCAACACGGCAGGGAAAAGCTCAACAAGTTCAACCAGATGATTGATGGGTGGCTCGCTAATAGTTCGTCGCCTGAAGAGATACGTGACGGATTAAAAAATCTTGAAAAGGATCAGGAGGGTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRAT

Protein Analysis

248

Amino Acids

28.36

Weight (kDa)

8.41

Isoelectric Point (pI)

31.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRAT PF04970 14 - 153 5e-25 Lecithin retinol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 20
AarI CACCTGC 1 cut(s) 326
Acc36I ACCTGC 1 cut(s) 326
AccB1I GGYRCC 1 cut(s) 314
AccII CGCG 2 cut(s) 13, 363
AclWI GGATC 1 cut(s) 738
AcoI YGGCCR 1 cut(s) 250
AcuI CTGAAG 1 cut(s) 714
AfaI GTAC 2 cut(s) 10, 89
AflII CTTAAG 1 cut(s) 410
AflIII ACRYGT 1 cut(s) 11
AgsI TTSAA 3 cut(s) 623, 652, 725
AjnI CCWGG 1 cut(s) 468
AluBI AGCT 1 cut(s) 640
AluI AGCT 1 cut(s) 640
Alw26I GTCTC 3 cut(s) 157, 419, 536
AlwI GGATC 1 cut(s) 738
Ama87I CYCGRG 1 cut(s) 44
AoxI GGCC 1 cut(s) 250
ApeKI GCWGC 1 cut(s) 4
AspLEI GCGC 2 cut(s) 365, 459
AsuHPI GGTGA 2 cut(s) 136, 197
AvaI CYCGRG 1 cut(s) 44
BaeI ACNNNNGTAYC 2 cut(s) 85, 118
BalI TGGCCA 1 cut(s) 252
BanI GGYRCC 1 cut(s) 314
BbsI GAAGAC 1 cut(s) 375
BbvI GCAGC 1 cut(s) 16
BccI CCATC 2 cut(s) 362, 659
BceAI ACGGC 2 cut(s) 568, 643
BcgI CGANNNNNNTGC 2 cut(s) 413, 447
BciT130I CCWGG 1 cut(s) 470
BclI TGATCA 1 cut(s) 492
BcoDI GTCTC 3 cut(s) 157, 419, 536
BfaI CTAG 1 cut(s) 608
BfrI CTTAAG 1 cut(s) 410
BfuAI ACCTGC 1 cut(s) 326
BglII AGATCT 1 cut(s) 610
BisI GCNGC 5 cut(s) 5, 265, 292, 361, 501
BlsI GCNGC 5 cut(s) 6, 266, 293, 362, 502
Bme1390I CCNGG 1 cut(s) 470
BmeT110I CYCGRG 1 cut(s) 44
BmiI GGNNCC 1 cut(s) 316
BmrFI CCNGG 1 cut(s) 470
BmsI GCATC 1 cut(s) 322
BpiI GAAGAC 1 cut(s) 375
BsaAI YACGTR 1 cut(s) 704
BsaI GGTCTC 1 cut(s) 419
BsaJI CCNNGG 3 cut(s) 97, 211, 307
Bse118I RCCGGY 1 cut(s) 326
BseBI CCWGG 1 cut(s) 470
BseDI CCNNGG 3 cut(s) 97, 211, 307
BseRI GAGGAG 1 cut(s) 228
BseXI GCAGC 1 cut(s) 16
BsgI GTGCAG 1 cut(s) 304
Bsh1236I CGCG 2 cut(s) 13, 363
BshFI GGCC 1 cut(s) 252
BshNI GGYRCC 1 cut(s) 314
BsiHKCI CYCGRG 1 cut(s) 44
BsiSI CCGG 1 cut(s) 327
BsiWI CGTACG 1 cut(s) 8
BslFI GGGAC 1 cut(s) 410
BsmAI GTCTC 3 cut(s) 157, 419, 536
BsmBI CGTCTC 1 cut(s) 157
BsmFI GGGAC 1 cut(s) 410
BsnI GGCC 1 cut(s) 252
Bso31I GGTCTC 1 cut(s) 419
BsoBI CYCGRG 1 cut(s) 44
Bsp143I GATC 6 cut(s) 27, 55, 492, 564, 610, 730
Bsp19I CCATGG 1 cut(s) 97
BspANI GGCC 1 cut(s) 252
BspFNI CGCG 2 cut(s) 13, 363
BspHI TCATGA 1 cut(s) 532
BspLI GGNNCC 1 cut(s) 316
BspMI ACCTGC 1 cut(s) 326
BspPI GGATC 1 cut(s) 738
BspT107I GGYRCC 1 cut(s) 314
BspTI CTTAAG 1 cut(s) 410
BspTNI GGTCTC 1 cut(s) 419
BsrFI RCCGGY 1 cut(s) 326
BssAI RCCGGY 1 cut(s) 326
BssECI CCNNGG 3 cut(s) 97, 211, 307
BssMI GATC 6 cut(s) 27, 55, 492, 564, 610, 730
BssT1I CCWWGG 2 cut(s) 97, 307
Bst2UI CCWGG 1 cut(s) 470
Bst4CI ACNGT 1 cut(s) 278
Bst6I CTCTTC 1 cut(s) 690
BstAFI CTTAAG 1 cut(s) 410
BstBAI YACGTR 1 cut(s) 704
BstC8I GCNNGC 2 cut(s) 582, 675
BstDSI CCRYGG 1 cut(s) 97
BstFNI CGCG 2 cut(s) 13, 363
BstHHI GCGC 2 cut(s) 365, 459
BstKTI GATC 6 cut(s) 30, 58, 495, 567, 613, 733
BstMAI GTCTC 3 cut(s) 157, 419, 536
BstMBI GATC 6 cut(s) 27, 55, 492, 564, 610, 730
BstMWI GCNNNNNNNGC 5 cut(s) 10, 186, 237, 264, 327
BstNI CCWGG 1 cut(s) 470
BstSCI CCNGG 1 cut(s) 468
BstUI CGCG 2 cut(s) 13, 363
BstV1I GCAGC 1 cut(s) 16
BstV2I GAAGAC 1 cut(s) 375
BstX2I RGATCY 1 cut(s) 610
BstYI RGATCY 1 cut(s) 610
BsuRI GGCC 1 cut(s) 252
BtgI CCRYGG 1 cut(s) 97
BtgZI GCGATG 2 cut(s) 272, 345
BtsIMutI CAGTG 1 cut(s) 283
BveI ACCTGC 1 cut(s) 326
Cac8I GCNNGC 2 cut(s) 582, 675
CciI TCATGA 1 cut(s) 532
CfoI GCGC 2 cut(s) 365, 459
Cfr10I RCCGGY 1 cut(s) 326
Csp6I GTAC 2 cut(s) 9, 88
CviAII CATG 3 cut(s) 98, 177, 533
CviJI RGCY 9 cut(s) 42, 84, 175, 252, 258, 360, 526, 640, 673
CviKI_1 RGCY 9 cut(s) 42, 84, 175, 252, 258, 360, 526, 640, 673
CviQI GTAC 2 cut(s) 9, 88
DpnI GATC 6 cut(s) 29, 57, 494, 566, 612, 732
DpnII GATC 6 cut(s) 27, 55, 492, 564, 610, 730
EaeI YGGCCR 1 cut(s) 250
Eam1104I CTCTTC 1 cut(s) 690
EarI CTCTTC 1 cut(s) 690
EciI GGCGGA 2 cut(s) 251, 488
Eco130I CCWWGG 2 cut(s) 97, 307
Eco31I GGTCTC 1 cut(s) 419
Eco57I CTGAAG 1 cut(s) 714
Eco88I CYCGRG 1 cut(s) 44
EcoRII CCWGG 1 cut(s) 468
EcoT14I CCWWGG 2 cut(s) 97, 307
ErhI CCWWGG 2 cut(s) 97, 307
Esp3I CGTCTC 1 cut(s) 157
FaeI CATG 3 cut(s) 101, 180, 536
FaiI YATR 8 cut(s) 20, 60, 66, 99, 178, 402, 485, 534
FalI AAGNNNNNCTT 2 cut(s) 68, 100
FaqI GGGAC 1 cut(s) 410
FatI CATG 3 cut(s) 97, 176, 532
FauI CCCGC 2 cut(s) 182, 348
FbaI TGATCA 1 cut(s) 492
Fnu4HI GCNGC 5 cut(s) 5, 265, 292, 361, 501
Fsp4HI GCNGC 5 cut(s) 5, 265, 292, 361, 501
FspBI CTAG 1 cut(s) 608
GlaI GCGC 2 cut(s) 364, 458
GluI GCNGC 5 cut(s) 5, 265, 292, 361, 501
HaeIII GGCC 1 cut(s) 252
HapII CCGG 1 cut(s) 327
HhaI GCGC 2 cut(s) 365, 459
Hin1II CATG 3 cut(s) 101, 180, 536
Hin6I GCGC 2 cut(s) 363, 457
HinP1I GCGC 2 cut(s) 363, 457
HincII GTYRAC 2 cut(s) 196, 235
HindII GTYRAC 2 cut(s) 196, 235
HinfI GANTC 5 cut(s) 127, 168, 216, 544, 619
HpaII CCGG 1 cut(s) 327
HphI GGTGA 2 cut(s) 136, 197
Hpy166II GTNNAC 2 cut(s) 196, 235
Hpy188I TCNGA 2 cut(s) 140, 212
Hpy188III TCNNGA 5 cut(s) 162, 533, 608, 722, 734
Hpy8I GTNNAC 2 cut(s) 196, 235
Hpy99I CGWCG 1 cut(s) 691
HpyCH4III ACNGT 1 cut(s) 278
HpyCH4IV ACGT 1 cut(s) 703
HpyCH4V TGCA 3 cut(s) 4, 321, 444
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 186, 237, 264, 327
HpySE526I ACGT 1 cut(s) 703
Hsp92II CATG 3 cut(s) 101, 180, 536
HspAI GCGC 2 cut(s) 363, 457
Ksp22I TGATCA 1 cut(s) 492
Kzo9I GATC 6 cut(s) 27, 55, 492, 564, 610, 730
LmnI GCTCC 1 cut(s) 462
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 1 cut(s) 322
MaeI CTAG 1 cut(s) 608
MaeII ACGT 1 cut(s) 703
MaeIII GTNAC 2 cut(s) 349, 704
MalI GATC 6 cut(s) 29, 57, 494, 566, 612, 732
MboI GATC 6 cut(s) 27, 55, 492, 564, 610, 730
MboII GAAGA 4 cut(s) 97, 128, 380, 707
MflI RGATCY 1 cut(s) 610
MlsI TGGCCA 1 cut(s) 252
MluI ACGCGT 1 cut(s) 11
MluNI TGGCCA 1 cut(s) 252
MlyI GAGTC 3 cut(s) 177, 225, 538
Mox20I TGGCCA 1 cut(s) 252
MscI TGGCCA 1 cut(s) 252
MseI TTAA 6 cut(s) 378, 411, 519, 575, 713, 745
MslI CAYNNNNRTG 1 cut(s) 63
Msp20I TGGCCA 1 cut(s) 252
MspCI CTTAAG 1 cut(s) 410
MspI CCGG 1 cut(s) 327
MspR9I CCNGG 1 cut(s) 470
MvaI CCWGG 1 cut(s) 470
MvnI CGCG 2 cut(s) 13, 363
MwoI GCNNNNNNNGC 5 cut(s) 10, 186, 237, 264, 327
NcoI CCATGG 1 cut(s) 97
NdeII GATC 6 cut(s) 27, 55, 492, 564, 610, 730
NlaIII CATG 3 cut(s) 101, 180, 536
NlaIV GGNNCC 1 cut(s) 316
NmuCI GTSAC 2 cut(s) 349, 704
PaeR7I CTCGAG 1 cut(s) 44
PagI TCATGA 1 cut(s) 532
PaqCI CACCTGC 1 cut(s) 326
PfeI GAWTC 2 cut(s) 127, 619
Pfl23II CGTACG 1 cut(s) 8
PkrI GCNGC 5 cut(s) 6, 266, 293, 362, 502
PleI GAGTC 3 cut(s) 176, 224, 538
PpsI GAGTC 3 cut(s) 176, 224, 538
Ppu21I YACGTR 1 cut(s) 704
PsiI TTATAA 1 cut(s) 20
Psp6I CCWGG 1 cut(s) 468
PspGI CCWGG 1 cut(s) 468
PspLI CGTACG 1 cut(s) 8
PspN4I GGNNCC 1 cut(s) 316
PspXI VCTCGAGB 1 cut(s) 44
PsuI RGATCY 1 cut(s) 610
RsaI GTAC 2 cut(s) 10, 89
RsaNI GTAC 2 cut(s) 9, 88
RseI CAYNNNNRTG 1 cut(s) 63
SaqAI TTAA 6 cut(s) 378, 411, 519, 575, 713, 745
SatI GCNGC 5 cut(s) 5, 265, 292, 361, 501
Sau3AI GATC 6 cut(s) 27, 55, 492, 564, 610, 730
SchI GAGTC 3 cut(s) 177, 225, 538
ScrFI CCNGG 1 cut(s) 470
SetI ASST 9 cut(s) 210, 309, 320, 375, 431, 513, 596, 642, 706
SfaNI GCATC 1 cut(s) 322
Sfr274I CTCGAG 1 cut(s) 44
SgrAI CRCCGGYG 1 cut(s) 326
SlaI CTCGAG 1 cut(s) 44
SmiMI CAYNNNNRTG 1 cut(s) 63
SmlI CTYRAG 2 cut(s) 44, 410
SmoI CTYRAG 2 cut(s) 44, 410
SspMI CTAG 1 cut(s) 608
StyD4I CCNGG 1 cut(s) 468
StyI CCWWGG 2 cut(s) 97, 307
TaaI ACNGT 1 cut(s) 278
TaiI ACGT 1 cut(s) 706
TaqI TCGA 5 cut(s) 30, 45, 161, 171, 617
TatI WGTACW 1 cut(s) 87
TauI GCSGC 4 cut(s) 267, 294, 363, 503
TfiI GAWTC 2 cut(s) 127, 619
Tru1I TTAA 6 cut(s) 378, 411, 519, 575, 713, 745
Tru9I TTAA 6 cut(s) 378, 411, 519, 575, 713, 745
TscAI CASTG 1 cut(s) 283
TseFI GTSAC 2 cut(s) 349, 704
TseI GCWGC 1 cut(s) 4
Tsp45I GTSAC 2 cut(s) 349, 704
TspDTI ATGAA 1 cut(s) 97
TspGWI ACGGA 1 cut(s) 723
TspRI CASTG 1 cut(s) 283
Vha464I CTTAAG 1 cut(s) 410
XbaI TCTAGA 1 cut(s) 607
XcmI CCANNNNNNNNNTGG 1 cut(s) 662
XhoI CTCGAG 1 cut(s) 44
XspI CTAG 1 cut(s) 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.