Rroxscaffold_4G00277260

NC domain-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0002037
Physical Location & Seq
Reverse (-)
704007 .. 716779
12773 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00277260.1

Sequence Viewer

Length: 720 bp
ATGCAGTATATTACAAACCTGATCAGCAAAAGGAACCTCGAGCGTGGAGATCATATTTATGCTTACAGAAAAGGTTACTTTTACACCCACCATGGTATCTTCGTTGGGAACGATACAGTGATTCACTTTAACATAACACAAGGAGACGCATCGAGAGGAGGTAGGCGATGCAGAAAGTGCGGGGTTGACAAAGATCACCTCCGAGGAGTCGTCAAGTCCTGCGTCGACTGCTTCCTCAATGGCCGCCACCTCCACCGCTTCCGATACAGTGTTGGTCCCATCCGCTTCGTTACCAACGTTGCAGGCACCTGCACCACCGGCAATGCTGACCCGCCAGATGTGACCATCAGCCGCGCCAATGGCCTGTTTGACAGGCAAGATTTTGGGGACTATGACTTGGCTCTGAACAACTGCGAGACCTTTGCGGTGTTCTGCAAGACGAGGGTCCGCAGCAGCGACCAGGCTCGCTCGGTTCTATCGCGGATTGATGCGGCGCTTGAGAGGCTTTATAATGATGTGTTGGTTCATCACGGCCAGGAAAAGCTCAACGAGTTAATGCAGGTGATTACCAGGTTTCTTGGTGATGAGCCGAGTCAACAGGGCGTGGGGAAGCTCAACAAGTTCAAGCAGAAGATTGATTGGTATCGCGCTAATAGGTGGTCGCCTGACCAGATCGGTGACAGTTTAAGAAATCTTGAAATTGATCAGGGGAATCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRAT

Protein Analysis

239

Amino Acids

27.34

Weight (kDa)

9.08

Isoelectric Point (pI)

30.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRAT PF04970 12 - 147 1.6e-21 Lecithin retinol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 510
AarI CACCTGC 2 cut(s) 317, 550
Acc36I ACCTGC 2 cut(s) 317, 550
AccB1I GGYRCC 1 cut(s) 305
AccI GTMKAC 1 cut(s) 225
AccII CGCG 3 cut(s) 354, 481, 648
AclI AACGTT 1 cut(s) 297
AcoI YGGCCR 2 cut(s) 241, 532
AgsI TTSAA 2 cut(s) 625, 698
AjnI CCWGG 3 cut(s) 459, 534, 569
AluBI AGCT 2 cut(s) 544, 613
AluI AGCT 2 cut(s) 544, 613
Alw26I GTCTC 2 cut(s) 138, 410
Ama87I CYCGRG 1 cut(s) 38
AoxI GGCC 3 cut(s) 241, 361, 532
ApeKI GCWGC 2 cut(s) 450, 453
AspLEI GCGC 3 cut(s) 356, 496, 650
AspS9I GGNCC 2 cut(s) 275, 445
AsuHPI GGTGA 4 cut(s) 188, 574, 593, 689
AvaI CYCGRG 1 cut(s) 38
AvaII GGWCC 2 cut(s) 275, 445
BaeI ACNNNNGTAYC 2 cut(s) 79, 112
BanI GGYRCC 1 cut(s) 305
BbvI GCAGC 2 cut(s) 462, 465
BccI CCATC 2 cut(s) 287, 353
BceAI ACGGC 1 cut(s) 547
BcgI CGANNNNNNTGC 2 cut(s) 404, 438
BciT130I CCWGG 3 cut(s) 461, 536, 571
BclI TGATCA 2 cut(s) 21, 703
BcoDI GTCTC 2 cut(s) 138, 410
BfoI RGCGCY 1 cut(s) 497
BfuAI ACCTGC 2 cut(s) 317, 550
BisI GCNGC 5 cut(s) 244, 352, 451, 454, 492
BlsI GCNGC 5 cut(s) 245, 353, 452, 455, 493
Bme1390I CCNGG 3 cut(s) 461, 536, 571
Bme18I GGWCC 2 cut(s) 275, 445
BmeT110I CYCGRG 1 cut(s) 38
BmgT120I GGNCC 2 cut(s) 275, 445
BmiI GGNNCC 4 cut(s) 35, 277, 307, 446
BmrFI CCNGG 3 cut(s) 461, 536, 571
BmsI GCATC 3 cut(s) 158, 158, 478
BoxI GACNNNNGTC 1 cut(s) 443
BpuEI CTTGAG 1 cut(s) 518
BsaBI GATNNNNATC 1 cut(s) 642
BsaI GGTCTC 1 cut(s) 410
BsaJI CCNNGG 2 cut(s) 91, 202
Bse118I RCCGGY 1 cut(s) 317
Bse3DI GCAATG 1 cut(s) 328
Bse8I GATNNNNATC 1 cut(s) 642
BseBI CCWGG 3 cut(s) 461, 536, 571
BseDI CCNNGG 2 cut(s) 91, 202
BseGI GGATG 1 cut(s) 279
BseJI GATNNNNATC 1 cut(s) 642
BseMI GCAATG 1 cut(s) 328
BseRI GAGGAG 2 cut(s) 171, 219
BseXI GCAGC 2 cut(s) 462, 465
BsgI GTGCAG 1 cut(s) 295
Bsh1236I CGCG 3 cut(s) 354, 481, 648
BshFI GGCC 3 cut(s) 243, 363, 534
BshNI GGYRCC 1 cut(s) 305
BsiHKCI CYCGRG 1 cut(s) 38
BsiSI CCGG 1 cut(s) 318
BslFI GGGAC 2 cut(s) 261, 401
BsmAI GTCTC 2 cut(s) 138, 410
BsmBI CGTCTC 1 cut(s) 138
BsmFI GGGAC 2 cut(s) 261, 401
BsnI GGCC 3 cut(s) 243, 363, 534
Bso31I GGTCTC 1 cut(s) 410
BsoBI CYCGRG 1 cut(s) 38
Bsp143I GATC 5 cut(s) 21, 49, 193, 672, 703
Bsp19I CCATGG 1 cut(s) 91
BspANI GGCC 3 cut(s) 243, 363, 534
BspFNI CGCG 3 cut(s) 354, 481, 648
BspLI GGNNCC 4 cut(s) 35, 277, 307, 446
BspMI ACCTGC 2 cut(s) 317, 550
BspT107I GGYRCC 1 cut(s) 305
BspTNI GGTCTC 1 cut(s) 410
BsrDI GCAATG 1 cut(s) 328
BsrFI RCCGGY 1 cut(s) 317
BssAI RCCGGY 1 cut(s) 317
BssECI CCNNGG 2 cut(s) 91, 202
BssMI GATC 5 cut(s) 21, 49, 193, 672, 703
BssT1I CCWWGG 1 cut(s) 91
Bst2UI CCWGG 3 cut(s) 461, 536, 571
Bst4CI ACNGT 3 cut(s) 118, 269, 683
BstAPI GCANNNNNTGC 1 cut(s) 177
BstC8I GCNNGC 2 cut(s) 304, 466
BstDSI CCRYGG 1 cut(s) 91
BstF5I GGATG 1 cut(s) 279
BstFNI CGCG 3 cut(s) 354, 481, 648
BstH2I RGCGCY 1 cut(s) 497
BstHHI GCGC 3 cut(s) 356, 496, 650
BstKTI GATC 5 cut(s) 24, 52, 196, 675, 706
BstMAI GTCTC 2 cut(s) 138, 410
BstMBI GATC 5 cut(s) 21, 49, 193, 672, 703
BstMWI GCNNNNNNNGC 5 cut(s) 177, 228, 318, 360, 502
BstNI CCWGG 3 cut(s) 461, 536, 571
BstPAI GACNNNNGTC 1 cut(s) 443
BstSCI CCNGG 3 cut(s) 459, 534, 569
BstUI CGCG 3 cut(s) 354, 481, 648
BstV1I GCAGC 2 cut(s) 462, 465
BsuRI GGCC 3 cut(s) 243, 363, 534
BtgI CCRYGG 1 cut(s) 91
BtgZI GCGATG 1 cut(s) 181
BtsCI GGATG 1 cut(s) 279
BtsIMutI CAGTG 2 cut(s) 123, 274
BveI ACCTGC 2 cut(s) 317, 550
Cac8I GCNNGC 2 cut(s) 304, 466
CfoI GCGC 3 cut(s) 356, 496, 650
Cfr10I RCCGGY 1 cut(s) 317
Cfr13I GGNCC 2 cut(s) 275, 445
CseI GACGC 2 cut(s) 155, 211
CsiI ACCWGGT 1 cut(s) 569
CviAII CATG 1 cut(s) 92
DpnI GATC 5 cut(s) 23, 51, 195, 674, 705
DpnII GATC 5 cut(s) 21, 49, 193, 672, 703
EaeI YGGCCR 2 cut(s) 241, 532
Eco130I CCWWGG 1 cut(s) 91
Eco31I GGTCTC 1 cut(s) 410
Eco47I GGWCC 2 cut(s) 275, 445
Eco88I CYCGRG 1 cut(s) 38
EcoRII CCWGG 3 cut(s) 459, 534, 569
EcoT14I CCWWGG 1 cut(s) 91
ErhI CCWWGG 1 cut(s) 91
Esp3I CGTCTC 1 cut(s) 138
FaeI CATG 1 cut(s) 95
FaiI YATR 7 cut(s) 9, 54, 60, 93, 134, 393, 510
FaqI GGGAC 2 cut(s) 261, 401
FatI CATG 1 cut(s) 91
FauI CCCGC 2 cut(s) 173, 339
FbaI TGATCA 2 cut(s) 21, 703
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 5 cut(s) 244, 352, 451, 454, 492
FokI GGATG 1 cut(s) 266
Fsp4HI GCNGC 5 cut(s) 244, 352, 451, 454, 492
GlaI GCGC 3 cut(s) 355, 495, 649
GluI GCNGC 5 cut(s) 244, 352, 451, 454, 492
HaeII RGCGCY 1 cut(s) 497
HaeIII GGCC 3 cut(s) 243, 363, 534
HapII CCGG 1 cut(s) 318
HgaI GACGC 2 cut(s) 155, 211
HhaI GCGC 3 cut(s) 356, 496, 650
Hin1II CATG 1 cut(s) 95
Hin6I GCGC 3 cut(s) 354, 494, 648
HinP1I GCGC 3 cut(s) 354, 494, 648
HincII GTYRAC 3 cut(s) 187, 226, 596
HindII GTYRAC 3 cut(s) 187, 226, 596
HinfI GANTC 4 cut(s) 121, 207, 592, 712
HpaII CCGG 1 cut(s) 318
HphI GGTGA 4 cut(s) 188, 574, 593, 689
Hpy166II GTNNAC 3 cut(s) 187, 226, 596
Hpy188I TCNGA 3 cut(s) 203, 263, 405
Hpy188III TCNNGA 2 cut(s) 153, 695
Hpy8I GTNNAC 3 cut(s) 187, 226, 596
Hpy99I CGWCG 1 cut(s) 227
HpyCH4III ACNGT 3 cut(s) 118, 269, 683
HpyCH4IV ACGT 1 cut(s) 297
HpyCH4V TGCA 6 cut(s) 4, 171, 302, 312, 435, 559
HpyF10VI GCNNNNNNNGC 5 cut(s) 177, 228, 318, 360, 502
HpySE526I ACGT 1 cut(s) 297
Hsp92II CATG 1 cut(s) 95
HspAI GCGC 3 cut(s) 354, 494, 648
Ksp22I TGATCA 2 cut(s) 21, 703
Kzo9I GATC 5 cut(s) 21, 49, 193, 672, 703
Lsp1109I GCAGC 2 cut(s) 462, 465
LweI GCATC 3 cut(s) 158, 158, 478
MabI ACCWGGT 1 cut(s) 569
MaeII ACGT 1 cut(s) 297
MaeIII GTNAC 4 cut(s) 74, 289, 340, 677
MalI GATC 5 cut(s) 23, 51, 195, 674, 705
MboI GATC 5 cut(s) 21, 49, 193, 672, 703
MboII GAAGA 2 cut(s) 91, 643
MluCI AATT 1 cut(s) 699
MlyI GAGTC 2 cut(s) 216, 601
MnlI CCTC 9 cut(s) 47, 149, 152, 197, 209, 245, 260, 435, 495
MseI TTAA 4 cut(s) 129, 554, 686, 718
MslI CAYNNNNRTG 1 cut(s) 57
MspI CCGG 1 cut(s) 318
MspR9I CCNGG 3 cut(s) 461, 536, 571
MvaI CCWGG 3 cut(s) 461, 536, 571
MvnI CGCG 3 cut(s) 354, 481, 648
MwoI GCNNNNNNNGC 5 cut(s) 177, 228, 318, 360, 502
NcoI CCATGG 1 cut(s) 91
NdeII GATC 5 cut(s) 21, 49, 193, 672, 703
NlaIII CATG 1 cut(s) 95
NlaIV GGNNCC 4 cut(s) 35, 277, 307, 446
NmeAIII GCCGAG 1 cut(s) 615
NmuCI GTSAC 2 cut(s) 340, 677
PaeR7I CTCGAG 1 cut(s) 38
PaqCI CACCTGC 2 cut(s) 317, 550
PcsI WCGNNNNNNNCGW 2 cut(s) 108, 294
PfeI GAWTC 2 cut(s) 121, 712
PkrI GCNGC 5 cut(s) 245, 353, 452, 455, 493
PleI GAGTC 2 cut(s) 215, 600
PpsI GAGTC 2 cut(s) 215, 600
PshAI GACNNNNGTC 1 cut(s) 443
PsiI TTATAA 1 cut(s) 510
Psp1406I AACGTT 1 cut(s) 297
Psp6I CCWGG 3 cut(s) 459, 534, 569
PspGI CCWGG 3 cut(s) 459, 534, 569
PspN4I GGNNCC 4 cut(s) 35, 277, 307, 446
PspPI GGNCC 2 cut(s) 275, 445
PspXI VCTCGAGB 1 cut(s) 38
RseI CAYNNNNRTG 1 cut(s) 57
SalI GTCGAC 1 cut(s) 224
SaqAI TTAA 4 cut(s) 129, 554, 686, 718
SatI GCNGC 5 cut(s) 244, 352, 451, 454, 492
Sau3AI GATC 5 cut(s) 21, 49, 193, 672, 703
Sau96I GGNCC 2 cut(s) 275, 445
SchI GAGTC 2 cut(s) 216, 601
ScrFI CCNGG 3 cut(s) 461, 536, 571
SexAI ACCWGGT 1 cut(s) 569
SfaNI GCATC 3 cut(s) 158, 158, 478
Sfr274I CTCGAG 1 cut(s) 38
SinI GGWCC 2 cut(s) 275, 445
SlaI CTCGAG 1 cut(s) 38
SmiMI CAYNNNNRTG 1 cut(s) 57
SmlI CTYRAG 2 cut(s) 38, 497
SmoI CTYRAG 2 cut(s) 38, 497
Sse9I AATT 1 cut(s) 699
StyD4I CCNGG 3 cut(s) 459, 534, 569
StyI CCWWGG 1 cut(s) 91
TaaI ACNGT 3 cut(s) 118, 269, 683
TaiI ACGT 1 cut(s) 300
TaqI TCGA 3 cut(s) 39, 152, 225
TasI AATT 1 cut(s) 699
TauI GCSGC 3 cut(s) 246, 354, 494
TfiI GAWTC 2 cut(s) 121, 712
Tru1I TTAA 4 cut(s) 129, 554, 686, 718
Tru9I TTAA 4 cut(s) 129, 554, 686, 718
TscAI CASTG 2 cut(s) 123, 274
TseFI GTSAC 2 cut(s) 340, 677
TseI GCWGC 2 cut(s) 450, 453
Tsp45I GTSAC 2 cut(s) 340, 677
TspDTI ATGAA 1 cut(s) 515
TspRI CASTG 2 cut(s) 123, 274
VpaK11BI GGWCC 2 cut(s) 275, 445
XhoI CTCGAG 1 cut(s) 38
XmiI GTMKAC 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.