Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0001601

Physical Location & Seq

Genomic Coordinates Reverse (-)

9672042 .. 9673483

Gene Structure 1442 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1119400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 960 bp

ATGGCTGCAGTAGGAAACCTTATAGGAGGAGGGATTGTAGGCACATTGTGTTCGGAGCTGTATAATCTTGTTGTTACTCTGATAAAAACGACTAACGAGTTCAGTCCCCTCCTCGAAGCGATCAAATTAACGGTTGGCGGTTTGGAGTCACTGATCCCACAGATAGAAGATCGAAATGAGAAACTGAAAATCTCAAACAAGGAGACAGAAAGGTTAAAGAAGGCCTTGACCGATGGCGTAGAGCTGGTTGGCGAATGCTCCAACAATCCCAAGTGGTACAAGAAGGCTAAATATACAGACAAACTCACCGCTTTGGACGAGGCTCTCGAACGGGAGTTAGATATGCTAACAGCGCATGCAGCGAGCGATACAAAAGAGGCCTTGCTTTTGACGAGGAAAAACCACGACGATCTTGTTAAATTGGCGAGGGATGGGAGAAAGGGGTTGGAAATGGCGAGGAAACACGGCGATCAACTAGTAGAATTGGCGAAGGATGGGAGCGAGACATTCGATACAGTAAAGGAAATAATGAATCTGATCTATCATTTTCTAGGGTATTGTATATCAGGCCTGATCGTAATTATTGTTTTCTGGGTAGTGCTGCACACATTTATTCTGCTTGGAGAGAGTAAGCGGAATGCAACAGCAGTACTGGACGGGATAATATTTCCCCGCTGTAAAGTCTGCAAATCGGTTGTGGCCCTTGTGGAGTGCGCGGGGTTATGTATATCTTTGGTCTGGACATATGCGAAGCTACTGCATGCGTATTTTCTCACTGCGTCATGCTTCTTCTTCCTCACTGCGTCATACTTCATCAGCTTCACTATGGTAATTCGTGATCAGTATAAACGAGGTTTCTCCCGCTCAGAGGTCTGCAGATTGCTTTTCCAGATTGGCTGCAATTCTATTTTCTTGTTCATCATCTGCTGGATTGTTTTCTTATGTAAATTTTTCGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04014 map04014

Pfam Domains

Protein Families

NB-ARC RPW8

Protein Analysis

320

Amino Acids

35.88

Weight (kDa)

7.91

Isoelectric Point (pI)

21.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RPW8	PF05659	6 - 135	3.6e-16	Arabidopsis broad-spectrum mildew resistance protein RPW8

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001601)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g15040 FvH4_5g03510 FvH4_5g03520 FvH4_5g03530 FvH4_5g03550
malus_domestica	MD02G1119400.v1.1
prunus_persica	Prupe.7G140300_v2.0.a1 Prupe.7G140400_v2.0.a1
pyrus_communis	pycom02g05540
rosa_chinensis	RchiOBHm_Chr2g0104371
rosa_laevigata	RLG00000004056 RLG00000004058 RLG00000004059 RLG00000017286
rosa_multiflora	Rmu_co8128798.1_g000001 Rmu_co8255317.1_g000001 Rmu_co8353357.1_g000001 Rmu_sc0003017.1_g000006 Rmu_sc0011198.1_g000003 Rmu_sc0011198.1_g000004 Rmu_sc0019298.1_g000002 Rmu_sc0019298.1_g000003 Rmu_ssc0000257.1_g000017
rosa_roxburghii	Rroxscaffold_2G00139120 Rroxscaffold_3G00259700
rosa_rugosa	Rorug02G0120100 Rorug02G0120100 Rorug07G0030900 Rorug07G0031600 Rorug07G0031600
rosa_samantha	Rh2AG168600 Rh2AG170200 Rh2BG165600 Rh2BG175800 Rh2CG174600 Rh2CG175200 Rh2DG174100 Rh2DG176200 Rh7AG157000 Rh7AG157200 Rh7BG158700 Rh7BG158800 Rh7BG356200 Rh7CG162500 Rh7CG162700 Rh7DG157900 Rh7DG158000 Rh7DG158200
rosa_wichuraiana	Rw7G013410 Rw7G013440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	864
AccII	CGCG	1 cut(s)	716
AciI	CCGC	6 cut(s)	138, 309, 634, 673, 716, 862
AclWI	GGATC	1 cut(s)	148
AcsI	RAATTY	1 cut(s)	947
AdeI	CACNNNGTG	1 cut(s)	48
AfaI	GTAC	2 cut(s)	278, 651
AfiI	CCNNNNNNNGG	1 cut(s)	868
AhlI	ACTAGT	1 cut(s)	475
AluBI	AGCT	4 cut(s)	58, 244, 754, 819
AluI	AGCT	4 cut(s)	58, 244, 754, 819
Alw26I	GTCTC	2 cut(s)	197, 497
AlwI	GGATC	1 cut(s)	148
AoxI	GGCC	4 cut(s)	222, 378, 568, 699
ApeKI	GCWGC	4 cut(s)	5, 359, 601, 897
ApoI	RAATTY	1 cut(s)	947
AspLEI	GCGC	2 cut(s)	355, 716
AspS9I	GGNCC	1 cut(s)	700
AsuHPI	GGTGA	1 cut(s)	298
BbvI	GCAGC	3 cut(s)	371, 588, 884
BccI	CCATC	3 cut(s)	227, 425, 488
BceAI	ACGGC	1 cut(s)	481
BcgI	CGANNNNNNTGC	2 cut(s)	739, 773
BclI	TGATCA	1 cut(s)	838
BcoDI	GTCTC	2 cut(s)	197, 497
BcuI	ACTAGT	1 cut(s)	475
BfaI	CTAG	2 cut(s)	476, 551
BfmI	CTRYAG	2 cut(s)	6, 874
BisI	GCNGC	4 cut(s)	6, 360, 602, 898
BlsI	GCNGC	4 cut(s)	7, 361, 603, 899
BmcAI	AGTACT	1 cut(s)	651
BmgT120I	GGNCC	1 cut(s)	700
BsaXI	ACNNNNNCTCC	2 cut(s)	21, 51
Bsc4I	CCNNNNNNNGG	1 cut(s)	868
Bse1I	ACTGG	1 cut(s)	657
BseGI	GGATG	2 cut(s)	436, 499
BseLI	CCNNNNNNNGG	1 cut(s)	868
BseMII	CTCAG	1 cut(s)	879
BseNI	ACTGG	1 cut(s)	657
BseRI	GAGGAG	2 cut(s)	42, 101
BseXI	GCAGC	3 cut(s)	371, 588, 884
BsgI	GTGCAG	1 cut(s)	587
Bsh1236I	CGCG	1 cut(s)	716
BshFI	GGCC	4 cut(s)	224, 380, 570, 701
BslFI	GGGAC	1 cut(s)	90
BslI	CCNNNNNNNGG	1 cut(s)	868
BsmAI	GTCTC	2 cut(s)	197, 497
BsmFI	GGGAC	1 cut(s)	90
BsmI	GAATGC	2 cut(s)	260, 643
BsnI	GGCC	4 cut(s)	224, 380, 570, 701
Bsp143I	GATC	8 cut(s)	120, 153, 169, 409, 469, 537, 573, 838
BspACI	CCGC	6 cut(s)	138, 309, 634, 673, 716, 862
BspANI	GGCC	4 cut(s)	224, 380, 570, 701
BspCNI	CTCAG	1 cut(s)	878
BspFNI	CGCG	1 cut(s)	716
BspMAI	CTGCAG	2 cut(s)	10, 878
BspPI	GGATC	1 cut(s)	148
BsrBI	CCGCTC	1 cut(s)	864
BsrI	ACTGG	1 cut(s)	657
BssMI	GATC	8 cut(s)	120, 153, 169, 409, 469, 537, 573, 838
Bst4CI	ACNGT	2 cut(s)	133, 517
BstC8I	GCNNGC	3 cut(s)	357, 364, 762
BstDEI	CTNAG	1 cut(s)	865
BstF5I	GGATG	2 cut(s)	436, 499
BstFNI	CGCG	1 cut(s)	716
BstHHI	GCGC	2 cut(s)	355, 716
BstKTI	GATC	8 cut(s)	123, 156, 172, 412, 472, 540, 576, 841
BstMAI	GTCTC	2 cut(s)	197, 497
BstMBI	GATC	8 cut(s)	120, 153, 169, 409, 469, 537, 573, 838
BstMWI	GCNNNNNNNGC	2 cut(s)	352, 359
BstNSI	RCATGY	2 cut(s)	359, 764
BstSFI	CTRYAG	2 cut(s)	6, 874
BstUI	CGCG	1 cut(s)	716
BstV1I	GCAGC	3 cut(s)	371, 588, 884
BsuRI	GGCC	4 cut(s)	224, 380, 570, 701
BtsCI	GGATG	2 cut(s)	436, 499
BtsI	GCAGTG	2 cut(s)	774, 798
BtsIMutI	CAGTG	3 cut(s)	149, 774, 798
Cac8I	GCNNGC	3 cut(s)	357, 364, 762
CfoI	GCGC	2 cut(s)	355, 716
Cfr13I	GGNCC	1 cut(s)	700
CseI	GACGC	2 cut(s)	768, 792
Csp6I	GTAC	2 cut(s)	277, 650
CviAII	CATG	3 cut(s)	356, 761, 783
CviQI	GTAC	2 cut(s)	277, 650
DdeI	CTNAG	1 cut(s)	865
DpnI	GATC	8 cut(s)	122, 155, 171, 411, 471, 539, 575, 840
DpnII	GATC	8 cut(s)	120, 153, 169, 409, 469, 537, 573, 838
DraIII	CACNNNGTG	1 cut(s)	48
Eco147I	AGGCCT	3 cut(s)	224, 380, 570
FaeI	CATG	3 cut(s)	359, 764, 786
FalI	AAGNNNNNCTT	2 cut(s)	209, 241
FaqI	GGGAC	1 cut(s)	90
FatI	CATG	3 cut(s)	355, 760, 782
FauI	CCCGC	3 cut(s)	680, 709, 869
FauNDI	CATATG	1 cut(s)	745
FbaI	TGATCA	1 cut(s)	838
Fnu4HI	GCNGC	4 cut(s)	6, 360, 602, 898
FokI	GGATG	2 cut(s)	443, 506
Fsp4HI	GCNGC	4 cut(s)	6, 360, 602, 898
FspBI	CTAG	2 cut(s)	476, 551
GlaI	GCGC	2 cut(s)	354, 715
GluI	GCNGC	4 cut(s)	6, 360, 602, 898
HaeIII	GGCC	4 cut(s)	224, 380, 570, 701
HgaI	GACGC	2 cut(s)	768, 792
HhaI	GCGC	2 cut(s)	355, 716
Hin1II	CATG	3 cut(s)	359, 764, 786
Hin6I	GCGC	2 cut(s)	353, 714
HinP1I	GCGC	2 cut(s)	353, 714
HinfI	GANTC	2 cut(s)	146, 532
HphI	GGTGA	1 cut(s)	298
Hpy188I	TCNGA	4 cut(s)	55, 81, 537, 868
Hpy188III	TCNNGA	4 cut(s)	326, 739, 836, 889
Hpy99I	CGWCG	1 cut(s)	410
HpyAV	CCTTC	3 cut(s)	214, 277, 484
HpyCH4III	ACNGT	2 cut(s)	133, 517
HpyCH4V	TGCA	8 cut(s)	8, 359, 604, 641, 687, 760, 876, 900
HpyF10VI	GCNNNNNNNGC	2 cut(s)	352, 359
HpyF3I	CTNAG	1 cut(s)	865
Hsp92II	CATG	3 cut(s)	359, 764, 786
HspAI	GCGC	2 cut(s)	353, 714
Ksp22I	TGATCA	1 cut(s)	838
Kzo9I	GATC	8 cut(s)	120, 153, 169, 409, 469, 537, 573, 838
LmnI	GCTCC	3 cut(s)	55, 263, 498
LpnPI	CCDG	8 cut(s)	230, 552, 577, 584, 638, 724, 902, 913
Lsp1109I	GCAGC	3 cut(s)	371, 588, 884
MaeI	CTAG	2 cut(s)	476, 551
MaeIII	GTNAC	2 cut(s)	73, 147
MalI	GATC	8 cut(s)	122, 155, 171, 411, 471, 539, 575, 840
MbiI	CCGCTC	1 cut(s)	864
MboI	GATC	8 cut(s)	120, 153, 169, 409, 469, 537, 573, 838
MboII	GAAGA	3 cut(s)	179, 781, 784
MluCI	AATT	7 cut(s)	125, 419, 482, 579, 831, 901, 947
MlyI	GAGTC	1 cut(s)	155
MmeI	TCCRAC	2 cut(s)	285, 426
MseI	TTAA	3 cut(s)	128, 215, 417
MspA1I	CMGCKG	1 cut(s)	675
Mva1269I	GAATGC	2 cut(s)	260, 643
MvnI	CGCG	1 cut(s)	716
MwoI	GCNNNNNNNGC	2 cut(s)	352, 359
NdeI	CATATG	1 cut(s)	745
NdeII	GATC	8 cut(s)	120, 153, 169, 409, 469, 537, 573, 838
NlaIII	CATG	3 cut(s)	359, 764, 786
NmuCI	GTSAC	1 cut(s)	147
NspI	RCATGY	2 cut(s)	359, 764
PaeI	GCATGC	2 cut(s)	359, 764
PceI	AGGCCT	3 cut(s)	224, 380, 570
PcsI	WCGNNNNNNNCGW	1 cut(s)	324
PctI	GAATGC	2 cut(s)	260, 643
PfeI	GAWTC	1 cut(s)	532
PkrI	GCNGC	4 cut(s)	7, 361, 603, 899
PleI	GAGTC	1 cut(s)	154
PpsI	GAGTC	1 cut(s)	154
PspPI	GGNCC	1 cut(s)	700
PstI	CTGCAG	2 cut(s)	10, 878
RsaI	GTAC	2 cut(s)	278, 651
RsaNI	GTAC	2 cut(s)	277, 650
SaqAI	TTAA	3 cut(s)	128, 215, 417
SatI	GCNGC	4 cut(s)	6, 360, 602, 898
Sau3AI	GATC	8 cut(s)	120, 153, 169, 409, 469, 537, 573, 838
Sau96I	GGNCC	1 cut(s)	700
ScaI	AGTACT	1 cut(s)	651
SchI	GAGTC	1 cut(s)	155
SetI	ASST	8 cut(s)	21, 60, 215, 246, 756, 821, 856, 873
SfcI	CTRYAG	2 cut(s)	6, 874
SpeI	ACTAGT	1 cut(s)	475
SphI	GCATGC	2 cut(s)	359, 764
Sse9I	AATT	7 cut(s)	125, 419, 482, 579, 831, 901, 947
SseBI	AGGCCT	3 cut(s)	224, 380, 570
SsiI	CCGC	6 cut(s)	138, 309, 634, 673, 716, 862
SspI	AATATT	1 cut(s)	666
SspMI	CTAG	2 cut(s)	476, 551
StuI	AGGCCT	3 cut(s)	224, 380, 570
TaaI	ACNGT	2 cut(s)	133, 517
TaqI	TCGA	5 cut(s)	114, 172, 327, 510, 954
TaqII	GACCGA	1 cut(s)	245
TasI	AATT	7 cut(s)	125, 419, 482, 579, 831, 901, 947
TatI	WGTACW	1 cut(s)	649
TfiI	GAWTC	1 cut(s)	532
Tru1I	TTAA	3 cut(s)	128, 215, 417
Tru9I	TTAA	3 cut(s)	128, 215, 417
TscAI	CASTG	3 cut(s)	156, 781, 805
TseFI	GTSAC	1 cut(s)	147
TseI	GCWGC	4 cut(s)	5, 359, 601, 897
Tsp45I	GTSAC	1 cut(s)	147
TspDTI	ATGAA	3 cut(s)	545, 802, 907
TspRI	CASTG	3 cut(s)	156, 781, 805
XapI	RAATTY	1 cut(s)	947
XceI	RCATGY	2 cut(s)	359, 764
XspI	CTAG	2 cut(s)	476, 551
ZrmI	AGTACT	1 cut(s)	651

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.