pycom02g05540

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001601
Physical Location & Seq
Forward (+)
3759601 .. 3761489
1889 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g05540.4

Sequence Viewer

Length: 1449 bp
ATGGCTGTAGTAGGGGAGCTTGTTGCTGGGGCTGCTCTTGGGACAGTATTTTCAGAGCTGTATAGAGGCGTTCAAAAACTCTGGAAAAGCAATAAGCCCCTCCTCGGTGATCTCCAAACCACGCTAGATTCTCTAAAACCAATTGTTGACGAGATGAGAGATTGCAACGACGAATTGGATCCCTCGTGCAAGGAAATAGCAGCTTTCCATAGCAAAATCGAAGAGGGCGTAAAGCTTGTTTGCAAGTTGTCAACAGTTGATAAGTGGTACAAAAACCGTACGTGCACCAAGCAACTTGTGGAACTGGACGGGTCTCTCAAAACACATTTAAAGGTACTAACCGTGCAGGCAGCAAGGGATGGGAAGAAAACTAGGATTTCGGCAAAGAAGATAGAGAAGCAGCTCAATCTAGTCATTGACGTGGTACGAAATCAAGTTGAAAATAAAGCTTGGCGCGCACCTCCACTAACAGTTGGTTCGGATGTCAAGTCAATGCTTGTGCAGACTGCTCCTGAAGGAAGTTTGAGAGACACAATATTGCGAACAGTTAAAGGGTTTCATCTTGAGAAAATCCTAGGCTCCAGCAGCAGTCCCATTCATATATCAGCTACACTGATGACCCTAGTAAAGATAAAGATAAAGATAGAACAGGAGAATAATATGCTGAAGTTTCCGCCCAAGCTCTATGATTTTGTTCTCCTGAAGAACTTCAGCATCACCAATTTTCATAAGCTATCTACCTTGCCTGAAGAGGTTGGAAACCTGATAAATTTAGAAGTGTTGAGGTTGAACTCCTGCTCAAAATTGTCGGAGTTACCAGGTTCAGTTACGAACCTCAACAAGTTAAACTTTCTCGACATATCTGACTGCTACAGTATTACGGAGTTGCCTGAAGACATAGGTAACATGTCCAGTTTAAGAAACCTCAACATGACGCGGTGCTCCAAAGTGAATAAGTTGCCCCTGTCAGTTGTGTATCTTGATCAGTTAGAAGGAGTGGTCTGTGATGAAGAAACGAAAAAGTTATGGGAACCCTACTTACCCTATCTTGAAAACTTACGAATGGAGATGGCCCAAGAAAATAGGTTCCTCAAACAGACTAATCTTATAGTCGTAAGGATGCAGAAGAGAGATGTGATGAATAAAATAGTTCAATCACATGTAAGCAGTGTTAGGTTCGACTTACAAGCAGAGGTGAAGGCACTCGGGGAGAAATTAACAGATAAACTTGACAATTACATGGCCCAAATTATCAACCACATACAAATAAAGAACGAGATGGACTGGAAGGGGATGAGCGAATTGGAGAGGGACGACATGAACATTTCGGAATCAACAATTGTTTCAAGAAGCATTGACAGTGAGGTCCAGGCTAGGGAACTGACTCTGAGAAAGTTTAATGCGTGGCTGGATGACCCAAGCGCGGGGTATATGCTAGCTATTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

483

Amino Acids

54.59

Weight (kDa)

7.97

Isoelectric Point (pI)

40.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1364
AccII CGCG 3 cut(s) 456, 937, 1424
AciI CCGC 3 cut(s) 674, 937, 1424
AclWI GGATC 2 cut(s) 173, 186
AcsI RAATTY 1 cut(s) 769
AcuI CTGAAG 6 cut(s) 534, 686, 694, 722, 768, 912
AfaI GTAC 4 cut(s) 269, 280, 336, 426
AfiI CCNNNNNNNGG 4 cut(s) 104, 1375, 1423, 1424
AflIII ACRYGT 2 cut(s) 906, 1159
AgsI TTSAA 6 cut(s) 74, 440, 790, 1052, 1154, 1347
AjiI CACGTC 1 cut(s) 421
AjnI CCWGG 2 cut(s) 817, 1368
Alw21I GWGCWC 2 cut(s) 287, 944
Alw26I GTCTC 2 cut(s) 318, 522
Alw44I GTGCAC 1 cut(s) 283
AlwI GGATC 2 cut(s) 173, 186
Ama87I CYCGRG 1 cut(s) 1205
AoxI GGCC 2 cut(s) 1071, 1242
ApaLI GTGCAC 1 cut(s) 283
ApeKI GCWGC 5 cut(s) 32, 200, 350, 400, 585
ApoI RAATTY 1 cut(s) 769
Asp700I GAANNNNTTC 1 cut(s) 707
AspA2I CCTAGG 1 cut(s) 574
AspLEI GCGC 3 cut(s) 456, 458, 1424
AspS9I GGNCC 3 cut(s) 1072, 1243, 1366
AsuHPI GGTGA 3 cut(s) 119, 709, 1207
AsuNHI GCTAGC 1 cut(s) 1435
AvaI CYCGRG 1 cut(s) 1205
AvaII GGWCC 1 cut(s) 1366
AvrII CCTAGG 1 cut(s) 574
BaeGI GKGCMC 1 cut(s) 287
BamHI GGATCC 1 cut(s) 178
BauI CACGAG 1 cut(s) 184
BbsI GAAGAC 1 cut(s) 900
Bbv12I GWGCWC 2 cut(s) 287, 944
BbvI GCAGC 5 cut(s) 19, 212, 362, 412, 597
BccI CCATC 3 cut(s) 353, 1063, 1273
BciT130I CCWGG 2 cut(s) 819, 1370
BclI TGATCA 1 cut(s) 982
BcoDI GTCTC 2 cut(s) 318, 522
BfaI CTAG 7 cut(s) 125, 372, 410, 575, 623, 1374, 1436
BfmI CTRYAG 2 cut(s) 6, 871
BisI GCNGC 5 cut(s) 33, 201, 351, 401, 586
BlnI CCTAGG 1 cut(s) 574
BlsI GCNGC 5 cut(s) 34, 202, 352, 402, 587
Bme1390I CCNGG 2 cut(s) 819, 1370
Bme18I GGWCC 1 cut(s) 1366
BmeT110I CYCGRG 1 cut(s) 1205
BmgBI CACGTC 1 cut(s) 421
BmgT120I GGNCC 3 cut(s) 1072, 1243, 1366
BmiI GGNNCC 4 cut(s) 180, 580, 1032, 1088
BmrFI CCNGG 2 cut(s) 819, 1370
BmsI GCATC 2 cut(s) 723, 1110
BmtI GCTAGC 1 cut(s) 1439
BpiI GAAGAC 1 cut(s) 900
BpmI CTGGAG 1 cut(s) 565
BpuEI CTTGAG 1 cut(s) 584
BsaAI YACGTR 1 cut(s) 282
BsaI GGTCTC 1 cut(s) 318
BsaJI CCNNGG 2 cut(s) 103, 574
Bsc4I CCNNNNNNNGG 4 cut(s) 104, 1375, 1423, 1424
Bse1I ACTGG 3 cut(s) 309, 912, 1289
BseBI CCWGG 2 cut(s) 819, 1370
BseDI CCNNGG 2 cut(s) 103, 574
BseGI GGATG 5 cut(s) 364, 487, 1125, 1299, 1417
BseLI CCNNNNNNNGG 4 cut(s) 104, 1375, 1423, 1424
BseMII CTCAG 1 cut(s) 1379
BseNI ACTGG 3 cut(s) 309, 912, 1289
BsePI GCGCGC 1 cut(s) 454
BseRI GAGGAG 1 cut(s) 92
BseSI GKGCMC 1 cut(s) 287
BseXI GCAGC 5 cut(s) 19, 212, 362, 412, 597
BseYI CCCAGC 1 cut(s) 26
BsgI GTGCAG 2 cut(s) 365, 521
Bsh1236I CGCG 3 cut(s) 456, 937, 1424
BshFI GGCC 2 cut(s) 1073, 1244
BsiHKAI GWGCWC 2 cut(s) 287, 944
BsiHKCI CYCGRG 1 cut(s) 1205
BsiWI CGTACG 1 cut(s) 278
BslFI GGGAC 3 cut(s) 55, 576, 1325
BslI CCNNNNNNNGG 4 cut(s) 104, 1375, 1423, 1424
BsmAI GTCTC 2 cut(s) 318, 522
BsmFI GGGAC 3 cut(s) 55, 576, 1325
BsnI GGCC 2 cut(s) 1073, 1244
Bso31I GGTCTC 1 cut(s) 318
BsoBI CYCGRG 1 cut(s) 1205
Bsp1286I GDGCHC 2 cut(s) 287, 944
Bsp143I GATC 3 cut(s) 109, 178, 982
BspACI CCGC 3 cut(s) 674, 937, 1424
BspANI GGCC 2 cut(s) 1073, 1244
BspCNI CTCAG 1 cut(s) 1380
BspFNI CGCG 3 cut(s) 456, 937, 1424
BspLI GGNNCC 4 cut(s) 180, 580, 1032, 1088
BspOI GCTAGC 1 cut(s) 1439
BspPI GGATC 2 cut(s) 173, 186
BspTNI GGTCTC 1 cut(s) 318
BsrI ACTGG 3 cut(s) 309, 912, 1289
BssECI CCNNGG 2 cut(s) 103, 574
BssHII GCGCGC 1 cut(s) 454
BssMI GATC 3 cut(s) 109, 178, 982
BssSI CACGAG 1 cut(s) 184
BssT1I CCWWGG 1 cut(s) 574
Bst2BI CACGAG 1 cut(s) 184
Bst2UI CCWGG 2 cut(s) 819, 1370
Bst4CI ACNGT 8 cut(s) 46, 256, 278, 343, 472, 547, 875, 1361
Bst6I CTCTTC 3 cut(s) 216, 744, 1121
BstBAI YACGTR 1 cut(s) 282
BstC8I GCNNGC 3 cut(s) 348, 456, 1437
BstDEI CTNAG 1 cut(s) 1388
BstF5I GGATG 5 cut(s) 364, 487, 1125, 1299, 1417
BstFNI CGCG 3 cut(s) 456, 937, 1424
BstHHI GCGC 3 cut(s) 456, 458, 1424
BstKTI GATC 3 cut(s) 112, 181, 985
BstMAI GTCTC 2 cut(s) 318, 522
BstMBI GATC 3 cut(s) 109, 178, 982
BstMWI GCNNNNNNNGC 3 cut(s) 32, 455, 585
BstNI CCWGG 2 cut(s) 819, 1370
BstNSI RCATGY 2 cut(s) 910, 1163
BstSCI CCNGG 2 cut(s) 817, 1368
BstSFI CTRYAG 2 cut(s) 6, 871
BstSLI GKGCMC 1 cut(s) 287
BstUI CGCG 3 cut(s) 456, 937, 1424
BstV1I GCAGC 5 cut(s) 19, 212, 362, 412, 597
BstV2I GAAGAC 1 cut(s) 900
BstX2I RGATCY 1 cut(s) 178
BstYI RGATCY 1 cut(s) 178
BsuRI GGCC 2 cut(s) 1073, 1244
BtrI CACGTC 1 cut(s) 421
BtsCI GGATG 5 cut(s) 364, 487, 1125, 1299, 1417
BtsI GCAGTG 1 cut(s) 1174
BtsIMutI CAGTG 3 cut(s) 611, 1174, 1366
Cac8I GCNNGC 3 cut(s) 348, 456, 1437
CfoI GCGC 3 cut(s) 456, 458, 1424
Cfr13I GGNCC 3 cut(s) 1072, 1243, 1366
CseI GACGC 1 cut(s) 943
CsiI ACCWGGT 1 cut(s) 817
Csp6I GTAC 4 cut(s) 268, 279, 335, 425
CviAII CATG 5 cut(s) 907, 931, 1160, 1240, 1318
CviQI GTAC 4 cut(s) 268, 279, 335, 425
DdeI CTNAG 1 cut(s) 1388
DpnI GATC 3 cut(s) 111, 180, 984
DpnII GATC 3 cut(s) 109, 178, 982
DraI TTTAAA 1 cut(s) 330
DrdI GACNNNNNNGTC 1 cut(s) 1364
DseDI GACNNNNNNGTC 1 cut(s) 1364
Eam1104I CTCTTC 3 cut(s) 216, 744, 1121
EarI CTCTTC 3 cut(s) 216, 744, 1121
EciI GGCGGA 1 cut(s) 663
Eco130I CCWWGG 1 cut(s) 574
Eco31I GGTCTC 1 cut(s) 318
Eco47I GGWCC 1 cut(s) 1366
Eco57I CTGAAG 6 cut(s) 534, 686, 694, 722, 768, 912
Eco88I CYCGRG 1 cut(s) 1205
EcoRII CCWGG 2 cut(s) 817, 1368
EcoT14I CCWWGG 1 cut(s) 574
ErhI CCWWGG 1 cut(s) 574
FaeI CATG 5 cut(s) 910, 934, 1163, 1243, 1321
FalI AAGNNNNNCTT 2 cut(s) 833, 865
FaqI GGGAC 3 cut(s) 55, 576, 1325
FatI CATG 5 cut(s) 906, 930, 1159, 1239, 1317
FauI CCCGC 1 cut(s) 1417
FbaI TGATCA 1 cut(s) 982
Fnu4HI GCNGC 5 cut(s) 33, 201, 351, 401, 586
FokI GGATG 5 cut(s) 371, 494, 1132, 1306, 1424
Fsp4HI GCNGC 5 cut(s) 33, 201, 351, 401, 586
FspBI CTAG 7 cut(s) 125, 372, 410, 575, 623, 1374, 1436
GlaI GCGC 3 cut(s) 455, 457, 1423
GluI GCNGC 5 cut(s) 33, 201, 351, 401, 586
GsaI CCCAGC 1 cut(s) 30
GsuI CTGGAG 1 cut(s) 565
HaeIII GGCC 2 cut(s) 1073, 1244
HgaI GACGC 1 cut(s) 943
HhaI GCGC 3 cut(s) 456, 458, 1424
Hin1II CATG 5 cut(s) 910, 934, 1163, 1243, 1321
Hin6I GCGC 3 cut(s) 454, 456, 1422
HinP1I GCGC 3 cut(s) 454, 456, 1422
HincII GTYRAC 2 cut(s) 148, 252
HindII GTYRAC 2 cut(s) 148, 252
HindIII AAGCTT 2 cut(s) 233, 447
HinfI GANTC 3 cut(s) 128, 1331, 1384
HphI GGTGA 3 cut(s) 119, 709, 1207
Hpy166II GTNNAC 3 cut(s) 148, 252, 285
Hpy188I TCNGA 6 cut(s) 55, 481, 811, 865, 1330, 1389
Hpy188III TCNNGA 8 cut(s) 82, 512, 563, 700, 854, 980, 1049, 1347
Hpy8I GTNNAC 3 cut(s) 148, 252, 285
Hpy99I CGWCG 1 cut(s) 173
HpyAV CCTTC 4 cut(s) 509, 986, 1192, 1282
HpyCH4III ACNGT 8 cut(s) 46, 256, 278, 343, 472, 547, 875, 1361
HpyCH4IV ACGT 2 cut(s) 281, 420
HpyCH4V TGCA 7 cut(s) 165, 189, 243, 285, 346, 502, 1123
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 455, 585
HpyF3I CTNAG 1 cut(s) 1388
HpySE526I ACGT 2 cut(s) 281, 420
Hsp92II CATG 5 cut(s) 910, 934, 1163, 1243, 1321
HspAI GCGC 3 cut(s) 454, 456, 1422
Ksp22I TGATCA 1 cut(s) 982
Kzo9I GATC 3 cut(s) 109, 178, 982
LmnI GCTCC 4 cut(s) 16, 514, 584, 947
Lsp1109I GCAGC 5 cut(s) 19, 212, 362, 412, 597
LweI GCATC 2 cut(s) 723, 1110
MabI ACCWGGT 1 cut(s) 817
MaeI CTAG 7 cut(s) 125, 372, 410, 575, 623, 1374, 1436
MaeII ACGT 2 cut(s) 281, 420
MaeIII GTNAC 3 cut(s) 813, 826, 902
MalI GATC 3 cut(s) 111, 180, 984
MboI GATC 3 cut(s) 109, 178, 982
MboII GAAGA 8 cut(s) 233, 376, 400, 715, 761, 905, 1022, 1138
MfeI CAATTG 2 cut(s) 141, 1338
MflI RGATCY 1 cut(s) 178
MhlI GDGCHC 2 cut(s) 287, 944
MlyI GAGTC 1 cut(s) 1378
MmeI TCCRAC 2 cut(s) 736, 789
MroXI GAANNNNTTC 1 cut(s) 707
MseI TTAA 6 cut(s) 329, 549, 845, 917, 1217, 1398
MslI CAYNNNNRTG 1 cut(s) 419
MspR9I CCNGG 2 cut(s) 819, 1370
MunI CAATTG 2 cut(s) 141, 1338
MvaI CCWGG 2 cut(s) 819, 1370
MvnI CGCG 3 cut(s) 456, 937, 1424
MwoI GCNNNNNNNGC 3 cut(s) 32, 455, 585
NdeII GATC 3 cut(s) 109, 178, 982
NheI GCTAGC 1 cut(s) 1435
NlaIII CATG 5 cut(s) 910, 934, 1163, 1243, 1321
NlaIV GGNNCC 4 cut(s) 180, 580, 1032, 1088
NspI RCATGY 2 cut(s) 910, 1163
PauI GCGCGC 1 cut(s) 454
PciI ACATGT 2 cut(s) 906, 1159
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PdmI GAANNNNTTC 1 cut(s) 707
PfeI GAWTC 2 cut(s) 128, 1331
Pfl23II CGTACG 1 cut(s) 278
PkrI GCNGC 5 cut(s) 34, 202, 352, 402, 587
PleI GAGTC 1 cut(s) 1378
PpsI GAGTC 1 cut(s) 1378
Ppu21I YACGTR 1 cut(s) 282
PscI ACATGT 2 cut(s) 906, 1159
Psp6I CCWGG 2 cut(s) 817, 1368
PspFI CCCAGC 1 cut(s) 26
PspGI CCWGG 2 cut(s) 817, 1368
PspLI CGTACG 1 cut(s) 278
PspN4I GGNNCC 4 cut(s) 180, 580, 1032, 1088
PspPI GGNCC 3 cut(s) 1072, 1243, 1366
PsrI GAACNNNNNNTAC 2 cut(s) 1023, 1055
PsuI RGATCY 1 cut(s) 178
PteI GCGCGC 1 cut(s) 454
RsaI GTAC 4 cut(s) 269, 280, 336, 426
RsaNI GTAC 4 cut(s) 268, 279, 335, 425
RseI CAYNNNNRTG 1 cut(s) 419
SaqAI TTAA 6 cut(s) 329, 549, 845, 917, 1217, 1398
SatI GCNGC 5 cut(s) 33, 201, 351, 401, 586
Sau3AI GATC 3 cut(s) 109, 178, 982
Sau96I GGNCC 3 cut(s) 1072, 1243, 1366
SchI GAGTC 1 cut(s) 1378
ScrFI CCNGG 2 cut(s) 819, 1370
SduI GDGCHC 2 cut(s) 287, 944
SexAI ACCWGGT 1 cut(s) 817
SfaNI GCATC 2 cut(s) 723, 1110
SfcI CTRYAG 2 cut(s) 6, 871
SinI GGWCC 1 cut(s) 1366
SmiMI CAYNNNNRTG 1 cut(s) 419
SmlI CTYRAG 1 cut(s) 563
SmoI CTYRAG 1 cut(s) 563
SsiI CCGC 3 cut(s) 674, 937, 1424
SspI AATATT 1 cut(s) 537
SspMI CTAG 7 cut(s) 125, 372, 410, 575, 623, 1374, 1436
StyD4I CCNGG 2 cut(s) 817, 1368
StyI CCWWGG 1 cut(s) 574
TaaI ACNGT 8 cut(s) 46, 256, 278, 343, 472, 547, 875, 1361
TaiI ACGT 2 cut(s) 284, 423
TaqI TCGA 3 cut(s) 219, 855, 1179
TfiI GAWTC 2 cut(s) 128, 1331
Tru1I TTAA 6 cut(s) 329, 549, 845, 917, 1217, 1398
Tru9I TTAA 6 cut(s) 329, 549, 845, 917, 1217, 1398
TscAI CASTG 3 cut(s) 618, 1174, 1366
TseI GCWGC 5 cut(s) 32, 200, 350, 400, 585
TspDTI ATGAA 7 cut(s) 548, 587, 716, 1023, 1154, 1334, 1434
TspGWI ACGGA 1 cut(s) 896
TspRI CASTG 3 cut(s) 618, 1174, 1366
VneI GTGCAC 1 cut(s) 283
VpaK11BI GGWCC 1 cut(s) 1366
XapI RAATTY 1 cut(s) 769
XceI RCATGY 2 cut(s) 910, 1163
XcmI CCANNNNNNNNNTGG 1 cut(s) 295
XmaJI CCTAGG 1 cut(s) 574
XmnI GAANNNNTTC 1 cut(s) 707
XspI CTAG 7 cut(s) 125, 372, 410, 575, 623, 1374, 1436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.