Rroxscaffold_2G00139120

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001601
Physical Location & Seq
Reverse (-)
77451609 .. 77453101
1493 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00139120.1

Sequence Viewer

Length: 654 bp
ATGGTTGGAGTAGTAGAAATTGTTGGAGGGGCTGCTCTGGGGACCCTATTCGAAAAGCTGTACAACGGCGTCGAGAAATTACTGATCAACAAGTCCACTCAGTGCACGCCCCTCCTAGAAAACATCAAATCCACGCTCTACTCTTTACAACCACTGATCAAACAGATAGAACACCACAACATCGAATTAAATCTCTCAAACAAGGAAGTAGAGGATTTCAAAAAGAAGATCTTGGAGGGCGTGGTGCTGGTTGATAAGTTCTCGAAAGTTCGTAAGTGGTACAAGAACCCTAGCTACACACACCAACTTCAGGAGTTGGATCGATCCCTAAAGAGGCAGTTAGAGATACTGACGGCGGAGGGAGTGAGGGATGGGAAGGAGACGTTGGTTTTGGCGAGGAAGATCTTGCTTTCGGTATGGAGTATGCAGACGGCGTTCAATGAAATTCACGAGTCGTGGATGGTCTTCGTGTTTGTTCTATCTTTGGCAAGTATGGCGTTCATGTTTATTGTCGCCGCAGGGACAAGTCGGTCTATATTTGGTGTAACTGGCAATGGTGTTTGGTTAGTCTGCAAATTGTTTTGGTCGTTGTTCCGTATTGTCCTGAAGTTTTTAGAAGTTATTTGTAAGATTGCAATGGTTCCACTCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

217

Amino Acids

24.66

Weight (kDa)

9.2

Isoelectric Point (pI)

33.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 6 - 130 1.2e-22 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 529
AciI CCGC 2 cut(s) 356, 516
AclWI GGATC 2 cut(s) 318, 327
AcsI RAATTY 1 cut(s) 444
AcuI CTGAAG 2 cut(s) 293, 626
AcyI GRCGYC 1 cut(s) 69
AdeI CACNNNGTG 1 cut(s) 102
AfaI GTAC 2 cut(s) 62, 281
AfiI CCNNNNNNNGG 2 cut(s) 310, 333
AgsI TTSAA 2 cut(s) 220, 439
AjuI GAANNNNNNNTTGG 2 cut(s) 368, 400
AluBI AGCT 3 cut(s) 58, 294, 651
AluI AGCT 3 cut(s) 58, 294, 651
Alw21I GWGCWC 1 cut(s) 107
Alw26I GTCTC 1 cut(s) 374
Alw44I GTGCAC 1 cut(s) 103
AlwI GGATC 2 cut(s) 318, 327
ApaLI GTGCAC 1 cut(s) 103
ApeKI GCWGC 1 cut(s) 32
ApoI RAATTY 1 cut(s) 444
ArsI GACNNNNNNTTYG 2 cut(s) 373, 405
AspS9I GGNCC 1 cut(s) 42
AsuII TTCGAA 1 cut(s) 51
AvaII GGWCC 1 cut(s) 42
BaeGI GKGCMC 1 cut(s) 107
BauI CACGAG 1 cut(s) 449
BbsI GAAGAC 1 cut(s) 457
Bbv12I GWGCWC 1 cut(s) 107
BbvI GCAGC 1 cut(s) 19
BccI CCATC 2 cut(s) 365, 454
BceAI ACGGC 3 cut(s) 82, 369, 447
BclI TGATCA 2 cut(s) 84, 156
BcoDI GTCTC 1 cut(s) 374
BfaI CTAG 2 cut(s) 116, 291
BglII AGATCT 2 cut(s) 228, 402
BisI GCNGC 2 cut(s) 33, 516
BlsI GCNGC 2 cut(s) 34, 517
Bme18I GGWCC 1 cut(s) 42
BmgT120I GGNCC 1 cut(s) 42
BmiI GGNNCC 3 cut(s) 43, 44, 642
BpiI GAAGAC 1 cut(s) 457
Bpu14I TTCGAA 1 cut(s) 51
Bsa29I ATCGAT 1 cut(s) 322
BsaHI GRCGYC 1 cut(s) 69
Bsc4I CCNNNNNNNGG 2 cut(s) 310, 333
Bse1I ACTGG 1 cut(s) 553
Bse3DI GCAATG 2 cut(s) 559, 642
BseCI ATCGAT 1 cut(s) 322
BseGI GGATG 2 cut(s) 376, 465
BseLI CCNNNNNNNGG 2 cut(s) 310, 333
BseMI GCAATG 2 cut(s) 559, 642
BseMII CTCAG 1 cut(s) 113
BseNI ACTGG 1 cut(s) 553
BseSI GKGCMC 1 cut(s) 107
BseXI GCAGC 1 cut(s) 19
BshVI ATCGAT 1 cut(s) 322
BsiHKAI GWGCWC 1 cut(s) 107
BslFI GGGAC 2 cut(s) 55, 535
BslI CCNNNNNNNGG 2 cut(s) 310, 333
BsmAI GTCTC 1 cut(s) 374
BsmBI CGTCTC 1 cut(s) 374
BsmFI GGGAC 2 cut(s) 55, 535
Bsp119I TTCGAA 1 cut(s) 51
Bsp1286I GDGCHC 1 cut(s) 107
Bsp1407I TGTACA 1 cut(s) 60
Bsp143I GATC 6 cut(s) 84, 156, 228, 319, 323, 402
BspACI CCGC 2 cut(s) 356, 516
BspCNI CTCAG 1 cut(s) 112
BspDI ATCGAT 1 cut(s) 322
BspLI GGNNCC 3 cut(s) 43, 44, 642
BspPI GGATC 2 cut(s) 318, 327
BspT104I TTCGAA 1 cut(s) 51
BsrDI GCAATG 2 cut(s) 559, 642
BsrGI TGTACA 1 cut(s) 60
BsrI ACTGG 1 cut(s) 553
BssMI GATC 6 cut(s) 84, 156, 228, 319, 323, 402
BssNI GRCGYC 1 cut(s) 69
BssSI CACGAG 1 cut(s) 449
Bst2BI CACGAG 1 cut(s) 449
BstACI GRCGYC 1 cut(s) 69
BstAUI TGTACA 1 cut(s) 60
BstBI TTCGAA 1 cut(s) 51
BstC8I GCNNGC 1 cut(s) 107
BstDEI CTNAG 2 cut(s) 99, 647
BstF5I GGATG 2 cut(s) 376, 465
BstKTI GATC 6 cut(s) 87, 159, 231, 322, 326, 405
BstMAI GTCTC 1 cut(s) 374
BstMBI GATC 6 cut(s) 84, 156, 228, 319, 323, 402
BstMWI GCNNNNNNNGC 1 cut(s) 494
BstSLI GKGCMC 1 cut(s) 107
BstV1I GCAGC 1 cut(s) 19
BstV2I GAAGAC 1 cut(s) 457
BstX2I RGATCY 2 cut(s) 228, 402
BstYI RGATCY 2 cut(s) 228, 402
Bsu15I ATCGAT 1 cut(s) 322
BsuTUI ATCGAT 1 cut(s) 322
BtsCI GGATG 2 cut(s) 376, 465
BtsIMutI CAGTG 2 cut(s) 107, 152
Cac8I GCNNGC 1 cut(s) 107
Cfr13I GGNCC 1 cut(s) 42
ClaI ATCGAT 1 cut(s) 322
CseI GACGC 1 cut(s) 58
Csp6I GTAC 2 cut(s) 61, 280
CviAII CATG 1 cut(s) 502
CviJI RGCY 4 cut(s) 32, 58, 294, 651
CviKI_1 RGCY 4 cut(s) 32, 58, 294, 651
CviQI GTAC 2 cut(s) 61, 280
DdeI CTNAG 2 cut(s) 99, 647
DpnI GATC 6 cut(s) 86, 158, 230, 321, 325, 404
DpnII GATC 6 cut(s) 84, 156, 228, 319, 323, 402
DraIII CACNNNGTG 1 cut(s) 102
DrdI GACNNNNNNGTC 1 cut(s) 529
DseDI GACNNNNNNGTC 1 cut(s) 529
EciI GGCGGA 1 cut(s) 371
Eco47I GGWCC 1 cut(s) 42
Eco57I CTGAAG 2 cut(s) 293, 626
EcoO109I RGGNCCY 1 cut(s) 42
Esp3I CGTCTC 1 cut(s) 374
FaeI CATG 1 cut(s) 505
FaiI YATR 5 cut(s) 418, 425, 494, 503, 536
FalI AAGNNNNNCTT 2 cut(s) 215, 247
FaqI GGGAC 2 cut(s) 55, 535
FatI CATG 1 cut(s) 501
FbaI TGATCA 2 cut(s) 84, 156
Fnu4HI GCNGC 2 cut(s) 33, 516
FokI GGATG 2 cut(s) 383, 472
Fsp4HI GCNGC 2 cut(s) 33, 516
FspBI CTAG 2 cut(s) 116, 291
GluI GCNGC 2 cut(s) 33, 516
HgaI GACGC 1 cut(s) 58
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 1 cut(s) 505
HinfI GANTC 1 cut(s) 452
Hpy166II GTNNAC 2 cut(s) 96, 105
Hpy188III TCNNGA 5 cut(s) 73, 262, 311, 449, 604
Hpy8I GTNNAC 2 cut(s) 96, 105
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 1 cut(s) 370
HpyCH4IV ACGT 1 cut(s) 383
HpyCH4V TGCA 4 cut(s) 105, 427, 573, 635
HpyF10VI GCNNNNNNNGC 1 cut(s) 494
HpyF3I CTNAG 2 cut(s) 99, 647
HpySE526I ACGT 1 cut(s) 383
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 1 cut(s) 505
KflI GGGWCCC 1 cut(s) 42
Ksp22I TGATCA 2 cut(s) 84, 156
Kzo9I GATC 6 cut(s) 84, 156, 228, 319, 323, 402
LpnPI CCDG 6 cut(s) 23, 233, 296, 504, 534, 617
Lsp1109I GCAGC 1 cut(s) 19
MaeI CTAG 2 cut(s) 116, 291
MaeII ACGT 1 cut(s) 383
MaeIII GTNAC 1 cut(s) 544
MalI GATC 6 cut(s) 86, 158, 230, 321, 325, 404
MboI GATC 6 cut(s) 84, 156, 228, 319, 323, 402
MboII GAAGA 3 cut(s) 238, 412, 457
MflI RGATCY 2 cut(s) 228, 402
MhlI GDGCHC 1 cut(s) 107
MluCI AATT 5 cut(s) 18, 77, 185, 444, 575
MlyI GAGTC 1 cut(s) 461
MmeI TCCRAC 2 cut(s) 4, 297
MnlI CCTC 8 cut(s) 20, 122, 205, 229, 327, 352, 360, 390
MseI TTAA 1 cut(s) 188
MspA1I CMGCKG 1 cut(s) 651
MwoI GCNNNNNNNGC 1 cut(s) 494
NdeII GATC 6 cut(s) 84, 156, 228, 319, 323, 402
NlaIII CATG 1 cut(s) 505
NlaIV GGNNCC 3 cut(s) 43, 44, 642
NspV TTCGAA 1 cut(s) 51
PkrI GCNGC 2 cut(s) 34, 517
PleI GAGTC 1 cut(s) 460
PpsI GAGTC 1 cut(s) 460
PpuMI RGGWCCY 1 cut(s) 42
Psp5II RGGWCCY 1 cut(s) 42
PspN4I GGNNCC 3 cut(s) 43, 44, 642
PspPI GGNCC 1 cut(s) 42
PspPPI RGGWCCY 1 cut(s) 42
PsrI GAACNNNNNNTAC 2 cut(s) 278, 310
PsuI RGATCY 2 cut(s) 228, 402
PvuII CAGCTG 1 cut(s) 651
RsaI GTAC 2 cut(s) 62, 281
RsaNI GTAC 2 cut(s) 61, 280
SaqAI TTAA 1 cut(s) 188
SatI GCNGC 2 cut(s) 33, 516
Sau3AI GATC 6 cut(s) 84, 156, 228, 319, 323, 402
Sau96I GGNCC 1 cut(s) 42
SchI GAGTC 1 cut(s) 461
SduI GDGCHC 1 cut(s) 107
SetI ASST 4 cut(s) 60, 296, 386, 653
SfuI TTCGAA 1 cut(s) 51
SinI GGWCC 1 cut(s) 42
Sse9I AATT 5 cut(s) 18, 77, 185, 444, 575
SsiI CCGC 2 cut(s) 356, 516
SspMI CTAG 2 cut(s) 116, 291
TaiI ACGT 1 cut(s) 386
TaqI TCGA 5 cut(s) 51, 72, 183, 263, 322
TaqII GACCGA 1 cut(s) 519
TasI AATT 5 cut(s) 18, 77, 185, 444, 575
TatI WGTACW 1 cut(s) 60
TauI GCSGC 1 cut(s) 518
Tru1I TTAA 1 cut(s) 188
Tru9I TTAA 1 cut(s) 188
TscAI CASTG 2 cut(s) 107, 159
TseI GCWGC 1 cut(s) 32
TspDTI ATGAA 2 cut(s) 456, 490
TspGWI ACGGA 1 cut(s) 584
TspRI CASTG 2 cut(s) 107, 159
VneI GTGCAC 1 cut(s) 103
VpaK11BI GGWCC 1 cut(s) 42
XapI RAATTY 1 cut(s) 444
XspI CTAG 2 cut(s) 116, 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.