RLG00000004058

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001601
Physical Location & Seq
Reverse (-)
55753544 .. 55755909
2366 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004058

Sequence Viewer

Length: 693 bp
ATGGCTAATGGGTTCCCTTGCGGACCTAATATTGTCAATGAGTTCCCACTGGGAACCTTCATATATGAAAAACTGGGGACAAGATACGAGTTGCTCGATGGAACACGGCAGACTTGTCTGAATTTGATGTTCGGTACCTCTTGTTGCAATTATGGCCGACTTGGTTTAGGGGCTGCTCTAGGAACAGCATTTATTGTGTTGTACGATGCGGTTGATGAAGCGACGGCGGGCAAGTTTAAGAAGTTTAAACCCCTCCTAGGAGCTCTCGAATCGACATTAGACTCTATACAACCACTTGTCATCAAGCAAATAGCAGATTACAATAACGTGAAACTGGAGCTCCCAAATGATGAACTAGAAAAGCACCAGAAACAAATGAGGGAGGCAAAGACCTCATTTCAAGCAAGGGATAGTAAGGAGAACTTGCTTGTGGCCAAGCAAACTGCCAAAAAACTTGTTGAATTCCAAGAGTTCAATGAAAGACTGTTAAGAGAACGGACGTTGCTGCAAGAAGACGCGAGAGATTTGCAGCAGAAGTTTGTTTTGCAACTGCAAAACAACGGGAAACAGCTGATTCAATTGCAACTGGTGATAGACATGATGATGCATCAAGAAGCAAAGGGAGAAACTGATGCCGGAACAGGAGGCCTTGAGGTGGAGACCTTCTTAATTCGATCTCATGCAAAACCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC RPW8

Protein Analysis

231

Amino Acids

25.89

Weight (kDa)

6.0

Isoelectric Point (pI)

30.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 57 - 131 8.6e-09 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 134
AccB1I GGYRCC 1 cut(s) 134
AccII CGCG 1 cut(s) 518
AciI CCGC 3 cut(s) 21, 209, 227
AcoI YGGCCR 2 cut(s) 154, 432
AcsI RAATTY 2 cut(s) 121, 461
AfaI GTAC 2 cut(s) 136, 203
AfiI CCNNNNNNNGG 2 cut(s) 257, 655
AgsI TTSAA 4 cut(s) 401, 461, 475, 578
AluBI AGCT 3 cut(s) 263, 340, 571
AluI AGCT 3 cut(s) 263, 340, 571
Alw21I GWGCWC 2 cut(s) 265, 342
Alw26I GTCTC 1 cut(s) 653
AoxI GGCC 3 cut(s) 154, 432, 646
ApeKI GCWGC 3 cut(s) 173, 505, 529
ApoI RAATTY 2 cut(s) 121, 461
Asp718I GGTACC 1 cut(s) 134
AspA2I CCTAGG 1 cut(s) 256
AspS9I GGNCC 1 cut(s) 23
AsuHPI GGTGA 1 cut(s) 601
AvaII GGWCC 1 cut(s) 23
AvrII CCTAGG 1 cut(s) 256
BalI TGGCCA 1 cut(s) 434
BanI GGYRCC 1 cut(s) 134
BanII GRGCYC 2 cut(s) 265, 342
BbsI GAAGAC 1 cut(s) 519
Bbv12I GWGCWC 2 cut(s) 265, 342
BbvI GCAGC 3 cut(s) 160, 492, 541
BccI CCATC 1 cut(s) 92
BceAI ACGGC 2 cut(s) 122, 240
BcgI CGANNNNNNTGC 2 cut(s) 508, 542
BcoDI GTCTC 1 cut(s) 653
BfaI CTAG 3 cut(s) 179, 257, 356
BisI GCNGC 3 cut(s) 174, 506, 530
BlnI CCTAGG 1 cut(s) 256
BlsI GCNGC 3 cut(s) 175, 507, 531
Bme18I GGWCC 1 cut(s) 23
BmgT120I GGNCC 1 cut(s) 23
BmiI GGNNCC 3 cut(s) 14, 55, 136
BmrI ACTGGG 2 cut(s) 59, 83
BmsI GCATC 4 cut(s) 196, 594, 616, 622
BmuI ACTGGG 2 cut(s) 59, 83
BpiI GAAGAC 1 cut(s) 519
BpmI CTGGAG 1 cut(s) 356
BpuEI CTTGAG 1 cut(s) 671
BsaI GGTCTC 1 cut(s) 653
BsaJI CCNNGG 1 cut(s) 256
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bsc4I CCNNNNNNNGG 2 cut(s) 257, 655
Bse1I ACTGG 4 cut(s) 54, 78, 339, 591
BseDI CCNNGG 1 cut(s) 256
BseLI CCNNNNNNNGG 2 cut(s) 257, 655
BseNI ACTGG 4 cut(s) 54, 78, 339, 591
BseXI GCAGC 3 cut(s) 160, 492, 541
Bsh1236I CGCG 1 cut(s) 518
BshFI GGCC 3 cut(s) 156, 434, 648
BshNI GGYRCC 1 cut(s) 134
BsiHKAI GWGCWC 2 cut(s) 265, 342
BsiSI CCGG 1 cut(s) 636
BslFI GGGAC 1 cut(s) 91
BslI CCNNNNNNNGG 2 cut(s) 257, 655
BsmAI GTCTC 1 cut(s) 653
BsmFI GGGAC 1 cut(s) 91
BsnI GGCC 3 cut(s) 156, 434, 648
Bso31I GGTCTC 1 cut(s) 653
Bsp1286I GDGCHC 2 cut(s) 265, 342
Bsp143I GATC 1 cut(s) 674
BspACI CCGC 3 cut(s) 21, 209, 227
BspANI GGCC 3 cut(s) 156, 434, 648
BspFNI CGCG 1 cut(s) 518
BspLI GGNNCC 3 cut(s) 14, 55, 136
BspT107I GGYRCC 1 cut(s) 134
BspTNI GGTCTC 1 cut(s) 653
BsrI ACTGG 4 cut(s) 54, 78, 339, 591
BssECI CCNNGG 1 cut(s) 256
BssMI GATC 1 cut(s) 674
BssT1I CCWWGG 1 cut(s) 256
Bst4CI ACNGT 1 cut(s) 486
BstC8I GCNNGC 1 cut(s) 229
BstDEI CTNAG 1 cut(s) 690
BstFNI CGCG 1 cut(s) 518
BstKTI GATC 1 cut(s) 677
BstMAI GTCTC 1 cut(s) 653
BstMBI GATC 1 cut(s) 674
BstMWI GCNNNNNNNGC 1 cut(s) 153
BstUI CGCG 1 cut(s) 518
BstV1I GCAGC 3 cut(s) 160, 492, 541
BstV2I GAAGAC 1 cut(s) 519
BsuRI GGCC 3 cut(s) 156, 434, 648
BtsIMutI CAGTG 1 cut(s) 47
Cac8I GCNNGC 1 cut(s) 229
Cfr13I GGNCC 1 cut(s) 23
CseI GACGC 1 cut(s) 524
Csp6I GTAC 2 cut(s) 135, 202
CviAII CATG 2 cut(s) 598, 680
CviJI RGCY 8 cut(s) 5, 156, 173, 263, 340, 434, 571, 648
CviKI_1 RGCY 8 cut(s) 5, 156, 173, 263, 340, 434, 571, 648
CviQI GTAC 2 cut(s) 135, 202
DdeI CTNAG 1 cut(s) 690
DpnI GATC 1 cut(s) 676
DpnII GATC 1 cut(s) 674
DraI TTTAAA 1 cut(s) 247
EaeI YGGCCR 2 cut(s) 154, 432
Ecl136II GAGCTC 2 cut(s) 263, 340
Eco130I CCWWGG 1 cut(s) 256
Eco147I AGGCCT 1 cut(s) 648
Eco24I GRGCYC 2 cut(s) 265, 342
Eco31I GGTCTC 1 cut(s) 653
Eco47I GGWCC 1 cut(s) 23
Eco53kI GAGCTC 2 cut(s) 263, 340
EcoICRI GAGCTC 2 cut(s) 263, 340
EcoRI GAATTC 1 cut(s) 461
EcoT14I CCWWGG 1 cut(s) 256
EcoT22I ATGCAT 1 cut(s) 609
EcoT38I GRGCYC 2 cut(s) 265, 342
ErhI CCWWGG 1 cut(s) 256
FaeI CATG 2 cut(s) 601, 683
FaiI YATR 7 cut(s) 62, 64, 66, 153, 287, 599, 681
FalI AAGNNNNNCTT 2 cut(s) 407, 439
FaqI GGGAC 1 cut(s) 91
FatI CATG 2 cut(s) 597, 679
FauI CCCGC 1 cut(s) 220
Fnu4HI GCNGC 3 cut(s) 174, 506, 530
FriOI GRGCYC 2 cut(s) 265, 342
Fsp4HI GCNGC 3 cut(s) 174, 506, 530
FspBI CTAG 3 cut(s) 179, 257, 356
GluI GCNGC 3 cut(s) 174, 506, 530
GsuI CTGGAG 1 cut(s) 356
HaeIII GGCC 3 cut(s) 156, 434, 648
HapII CCGG 1 cut(s) 636
HgaI GACGC 1 cut(s) 524
Hin1II CATG 2 cut(s) 601, 683
HinfI GANTC 3 cut(s) 269, 281, 574
HpaII CCGG 1 cut(s) 636
HphI GGTGA 1 cut(s) 601
Hpy188I TCNGA 1 cut(s) 120
Hpy188III TCNNGA 2 cut(s) 266, 611
Hpy99I CGWCG 1 cut(s) 226
HpyAV CCTTC 2 cut(s) 67, 673
HpyCH4III ACNGT 1 cut(s) 486
HpyCH4IV ACGT 2 cut(s) 327, 500
HpyCH4V TGCA 8 cut(s) 147, 508, 529, 547, 553, 583, 607, 683
HpyF10VI GCNNNNNNNGC 1 cut(s) 153
HpyF3I CTNAG 1 cut(s) 690
HpySE526I ACGT 2 cut(s) 327, 500
Hsp92II CATG 2 cut(s) 601, 683
KpnI GGTACC 1 cut(s) 138
Kzo9I GATC 1 cut(s) 674
LmnI GCTCC 3 cut(s) 260, 337, 345
LpnPI CCDG 7 cut(s) 35, 59, 320, 380, 572, 627, 649
Lsp1109I GCAGC 3 cut(s) 160, 492, 541
LweI GCATC 4 cut(s) 196, 594, 616, 622
MaeI CTAG 3 cut(s) 179, 257, 356
MaeII ACGT 2 cut(s) 327, 500
MalI GATC 1 cut(s) 676
MboI GATC 1 cut(s) 674
MboII GAAGA 1 cut(s) 524
MfeI CAATTG 1 cut(s) 578
MhlI GDGCHC 2 cut(s) 265, 342
MlsI TGGCCA 1 cut(s) 434
MluCI AATT 5 cut(s) 121, 148, 461, 578, 669
MluNI TGGCCA 1 cut(s) 434
MlyI GAGTC 1 cut(s) 275
MnlI CCTC 7 cut(s) 148, 263, 372, 376, 403, 638, 646
Mox20I TGGCCA 1 cut(s) 434
Mph1103I ATGCAT 1 cut(s) 609
MscI TGGCCA 1 cut(s) 434
MseI TTAA 4 cut(s) 237, 246, 488, 668
MslI CAYNNNNRTG 1 cut(s) 602
Msp20I TGGCCA 1 cut(s) 434
MspA1I CMGCKG 1 cut(s) 571
MspI CCGG 1 cut(s) 636
MssI GTTTAAAC 1 cut(s) 247
MunI CAATTG 1 cut(s) 578
MvnI CGCG 1 cut(s) 518
MwoI GCNNNNNNNGC 1 cut(s) 153
NdeII GATC 1 cut(s) 674
NlaIII CATG 2 cut(s) 601, 683
NlaIV GGNNCC 3 cut(s) 14, 55, 136
NsiI ATGCAT 1 cut(s) 609
PceI AGGCCT 1 cut(s) 648
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PfeI GAWTC 2 cut(s) 269, 574
PkrI GCNGC 3 cut(s) 175, 507, 531
PleI GAGTC 1 cut(s) 275
PmeI GTTTAAAC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 275
Psp124BI GAGCTC 2 cut(s) 265, 342
PspN4I GGNNCC 3 cut(s) 14, 55, 136
PspPI GGNCC 1 cut(s) 23
PvuII CAGCTG 1 cut(s) 571
RsaI GTAC 2 cut(s) 136, 203
RsaNI GTAC 2 cut(s) 135, 202
RseI CAYNNNNRTG 1 cut(s) 602
SacI GAGCTC 2 cut(s) 265, 342
SaqAI TTAA 4 cut(s) 237, 246, 488, 668
SatI GCNGC 3 cut(s) 174, 506, 530
Sau3AI GATC 1 cut(s) 674
Sau96I GGNCC 1 cut(s) 23
SchI GAGTC 1 cut(s) 275
SduI GDGCHC 2 cut(s) 265, 342
SfaNI GCATC 4 cut(s) 196, 594, 616, 622
SinI GGWCC 1 cut(s) 23
SmiMI CAYNNNNRTG 1 cut(s) 602
SmlI CTYRAG 1 cut(s) 650
SmoI CTYRAG 1 cut(s) 650
Sse9I AATT 5 cut(s) 121, 148, 461, 578, 669
SseBI AGGCCT 1 cut(s) 648
SsiI CCGC 3 cut(s) 21, 209, 227
SspI AATATT 1 cut(s) 31
SspMI CTAG 3 cut(s) 179, 257, 356
SstI GAGCTC 2 cut(s) 265, 342
StuI AGGCCT 1 cut(s) 648
StyI CCWWGG 1 cut(s) 256
TaaI ACNGT 1 cut(s) 486
TaiI ACGT 2 cut(s) 330, 503
TaqI TCGA 4 cut(s) 96, 267, 272, 673
TasI AATT 5 cut(s) 121, 148, 461, 578, 669
TfiI GAWTC 2 cut(s) 269, 574
Tru1I TTAA 4 cut(s) 237, 246, 488, 668
Tru9I TTAA 4 cut(s) 237, 246, 488, 668
TscAI CASTG 1 cut(s) 54
TseI GCWGC 3 cut(s) 173, 505, 529
TspDTI ATGAA 5 cut(s) 49, 81, 231, 366, 492
TspGWI ACGGA 1 cut(s) 511
TspRI CASTG 1 cut(s) 54
VpaK11BI GGWCC 1 cut(s) 23
XapI RAATTY 2 cut(s) 121, 461
XmaJI CCTAGG 1 cut(s) 256
XspI CTAG 3 cut(s) 179, 257, 356
Zsp2I ATGCAT 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.