MD02G1185700.v1.1

Protein of unknown function (DUF568)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002816
Physical Location & Seq
Reverse (-)
16856473 .. 16857636
1164 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1185700.v1.1.491

Sequence Viewer

Length: 1164 bp
ATGGCACTTTCCAAGTCTCTCTTCGTTATTTGCTTACTGTTTACTGTCTCCTCCGCCGCCGCACAACCGCCATGCATGACCCGTATCTTCTCCAACAAGAAAACCTTCGCCGCATGCAGTACTCTGCCCGTTTTGAACTCGACGATCCACTGGAATTACTATCCGTTGACAGCCACGGTTGACATTGCATTCACACAATCTGTGGTGAGTGACTCGAGATGGGTTGCATGGGCCATAAACCCTACATCTACTGGCATGGTCGGTTCACAAGCCATTGTCGCTTACAAAAGAACCGATGGAACTATGACGGTTTATTCTTCACCAATTAAAAGTTACGGTACTCATTTAGAACAAGGGAATGTAAGCTTCCCTTTGTACGACGTCTCCGCGGTTTATGAGAACAACGAATTCATCATCTTTGCGACCATAGGCCTTCCGAACAATGTCAGCGTTGTGCACCACGTCTGGCAGCAAGGACCCATGTTCGGGAACACCCCCGGAATGCACTCGCTTTCTGGACCCAATGTGCAATCTTTTGGAACCTTAGATTTTCTTTCCGGGAAAACTGAAACAAGCAAGGGAAGTACTTCAACGTCCGCCTTAAAATATGCGCATGGAATTATCAATACCATCAGTTGGGGCATACTTATGCCCATTGGCATAATCGTGGCAAGGTATCTCAAGACGGTTGAAGGGGCCGATCCGGCATGGTTTCATGCTCATAGAGGATGCCAAATGCTGGCTTTTCTTGGAGGGATTGCAGGGTGGGGAACTGGAATTTTTCTTGGCTCAAAATCTCCAGGGATTCAGTACAAAGGGCATAAATGCATCGGCATCACTCTGTTTGTCCTTGCTACGATTCAAGTAGCGGTAGCTCTTTGTTTGAGGCCGAAGAAGACAGACGAGAATAGGAAATATTGGAACTGGTTTCATTGGGTTGTGGGGTATGGAACAATCGTCCTAAGCATCACCAATATTTTCAAAGGATTTGACATATTGGAGCCGGCCAACAAGTGGAAATTTGCTTACATTGCCATCATCGGCACTTTGGGATGCATCGCTGCAATCCTAGAGGTACGAAAACTAGGCTCTTTCCTTTCGAGGTTTAGAAGCAGTCGAGCTGCAGGGCCAGAAATCGTCGAGAGTACTCAAACAATAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561 DUF568

Protein Analysis

388

Amino Acids

42.17

Weight (kDa)

9.37

Isoelectric Point (pI)

29.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF568 PF04526 86 - 184 1.2e-25 Protein of unknown function (DUF568)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 384
Acc16I TGCGCA 1 cut(s) 612
AccB7I CCANNNNNTGG 3 cut(s) 636, 739, 1014
AccII CGCG 1 cut(s) 389
AciI CCGC 9 cut(s) 54, 57, 60, 68, 111, 387, 389, 597, 869
AclWI GGATC 2 cut(s) 139, 695
AcoI YGGCCR 1 cut(s) 1005
AcsI RAATTY 3 cut(s) 407, 777, 1019
AcyI GRCGYC 1 cut(s) 381
AfaI GTAC 7 cut(s) 121, 340, 377, 586, 812, 1077, 1147
AfiI CCNNNNNNNGG 5 cut(s) 485, 486, 636, 739, 1014
AgsI TTSAA 5 cut(s) 136, 591, 692, 863, 982
AjiI CACGTC 1 cut(s) 463
AjnI CCWGG 1 cut(s) 799
AjuI GAANNNNNNNTTGG 1 cut(s) 37
AluBI AGCT 3 cut(s) 366, 875, 1121
AluI AGCT 3 cut(s) 366, 875, 1121
Alw21I GWGCWC 1 cut(s) 459
Alw26I GTCTC 3 cut(s) 21, 52, 388
Alw44I GTGCAC 1 cut(s) 455
AlwI GGATC 2 cut(s) 139, 695
Ama87I CYCGRG 1 cut(s) 214
AoxI GGCC 6 cut(s) 231, 430, 696, 887, 1005, 1127
ApaLI GTGCAC 1 cut(s) 455
ApeKI GCWGC 3 cut(s) 469, 1061, 1121
ApoI RAATTY 3 cut(s) 407, 777, 1019
Asp700I GAANNNNTTC 2 cut(s) 104, 586
AspLEI GCGC 1 cut(s) 613
AspS9I GGNCC 5 cut(s) 231, 476, 518, 696, 1127
AsuC2I CCSGG 2 cut(s) 498, 559
AsuHPI GGTGA 3 cut(s) 217, 312, 961
AvaI CYCGRG 1 cut(s) 214
AvaII GGWCC 2 cut(s) 476, 518
BaeGI GKGCMC 1 cut(s) 459
BbsI GAAGAC 1 cut(s) 902
Bbv12I GWGCWC 1 cut(s) 459
BbvI GCAGC 3 cut(s) 481, 1048, 1108
BccI CCATC 4 cut(s) 213, 290, 638, 1043
BciT130I CCWGG 1 cut(s) 801
BcnI CCSGG 2 cut(s) 498, 559
BcoDI GTCTC 3 cut(s) 21, 52, 388
BfaI CTAG 2 cut(s) 1070, 1085
BfmI CTRYAG 1 cut(s) 1122
BglI GCCNNNNNGGC 1 cut(s) 704
BisI GCNGC 6 cut(s) 57, 60, 111, 470, 1062, 1122
BlsI GCNGC 6 cut(s) 58, 61, 112, 471, 1063, 1123
BmcAI AGTACT 3 cut(s) 121, 586, 1147
Bme1390I CCNGG 3 cut(s) 498, 559, 801
Bme18I GGWCC 2 cut(s) 476, 518
BmeT110I CYCGRG 1 cut(s) 214
BmgBI CACGTC 1 cut(s) 463
BmgT120I GGNCC 5 cut(s) 231, 476, 518, 696, 1127
BmiI GGNNCC 5 cut(s) 478, 520, 541, 697, 1002
BmrFI CCNGG 3 cut(s) 498, 559, 801
BmsI GCATC 6 cut(s) 719, 837, 843, 975, 1043, 1065
BpiI GAAGAC 1 cut(s) 902
BpmI CTGGAG 1 cut(s) 783
Bpu10I CCTNAGC 1 cut(s) 962
BpuEI CTTGAG 1 cut(s) 665
BpuMI CCSGG 2 cut(s) 498, 559
BsaHI GRCGYC 1 cut(s) 381
BsaJI CCNNGG 4 cut(s) 174, 387, 496, 800
BsaXI ACNNNNNCTCC 2 cut(s) 368, 398
Bsc4I CCNNNNNNNGG 5 cut(s) 485, 486, 636, 739, 1014
Bse118I RCCGGY 1 cut(s) 1003
Bse1I ACTGG 4 cut(s) 155, 256, 778, 929
Bse3DI GCAATG 2 cut(s) 183, 1029
BseBI CCWGG 1 cut(s) 801
BseDI CCNNGG 4 cut(s) 174, 387, 496, 800
BseGI GGATG 2 cut(s) 734, 1058
BseLI CCNNNNNNNGG 5 cut(s) 485, 486, 636, 739, 1014
BseMI GCAATG 2 cut(s) 183, 1029
BseNI ACTGG 4 cut(s) 155, 256, 778, 929
BseRI GAGGAG 1 cut(s) 40
BseSI GKGCMC 1 cut(s) 459
BseXI GCAGC 3 cut(s) 481, 1048, 1108
Bsh1236I CGCG 1 cut(s) 389
BshFI GGCC 6 cut(s) 233, 432, 698, 889, 1007, 1129
BsiHKAI GWGCWC 1 cut(s) 459
BsiHKCI CYCGRG 1 cut(s) 214
BsiSI CCGG 4 cut(s) 498, 558, 704, 1004
BslI CCNNNNNNNGG 5 cut(s) 485, 486, 636, 739, 1014
BsmAI GTCTC 3 cut(s) 21, 52, 388
BsmBI CGTCTC 1 cut(s) 388
BsmI GAATGC 2 cut(s) 188, 507
BsnI GGCC 6 cut(s) 233, 432, 698, 889, 1007, 1129
BsoBI CYCGRG 1 cut(s) 214
Bsp1286I GDGCHC 1 cut(s) 459
Bsp143I GATC 2 cut(s) 144, 700
BspACI CCGC 9 cut(s) 54, 57, 60, 68, 111, 387, 389, 597, 869
BspANI GGCC 6 cut(s) 233, 432, 698, 889, 1007, 1129
BspFNI CGCG 1 cut(s) 389
BspLI GGNNCC 5 cut(s) 478, 520, 541, 697, 1002
BspMAI CTGCAG 1 cut(s) 1126
BspPI GGATC 2 cut(s) 139, 695
BsrDI GCAATG 2 cut(s) 183, 1029
BsrFI RCCGGY 1 cut(s) 1003
BsrI ACTGG 4 cut(s) 155, 256, 778, 929
BssAI RCCGGY 1 cut(s) 1003
BssECI CCNNGG 4 cut(s) 174, 387, 496, 800
BssMI GATC 2 cut(s) 144, 700
BssNI GRCGYC 1 cut(s) 381
Bst2UI CCWGG 1 cut(s) 801
Bst4CI ACNGT 6 cut(s) 39, 46, 178, 310, 338, 688
Bst6I CTCTTC 1 cut(s) 26
BstACI GRCGYC 1 cut(s) 381
BstC8I GCNNGC 3 cut(s) 115, 741, 1005
BstDEI CTNAG 2 cut(s) 544, 962
BstDSI CCRYGG 2 cut(s) 174, 387
BstF5I GGATG 2 cut(s) 734, 1058
BstFNI CGCG 1 cut(s) 389
BstHHI GCGC 1 cut(s) 613
BstKTI GATC 2 cut(s) 147, 703
BstMAI GTCTC 3 cut(s) 21, 52, 388
BstMBI GATC 2 cut(s) 144, 700
BstMWI GCNNNNNNNGC 3 cut(s) 278, 704, 1031
BstNI CCWGG 1 cut(s) 801
BstNSI RCATGY 1 cut(s) 117
BstSCI CCNGG 3 cut(s) 496, 557, 799
BstSFI CTRYAG 1 cut(s) 1122
BstSLI GKGCMC 1 cut(s) 459
BstUI CGCG 1 cut(s) 389
BstV1I GCAGC 3 cut(s) 481, 1048, 1108
BstV2I GAAGAC 1 cut(s) 902
BsuRI GGCC 6 cut(s) 233, 432, 698, 889, 1007, 1129
BtgI CCRYGG 2 cut(s) 174, 387
BtgZI GCGATG 1 cut(s) 1042
BtrI CACGTC 1 cut(s) 463
BtsCI GGATG 2 cut(s) 734, 1058
BtsIMutI CAGTG 1 cut(s) 148
Cac8I GCNNGC 3 cut(s) 115, 741, 1005
CfoI GCGC 1 cut(s) 613
Cfr10I RCCGGY 1 cut(s) 1003
Cfr13I GGNCC 5 cut(s) 231, 476, 518, 696, 1127
Cfr42I CCGCGG 1 cut(s) 390
Csp6I GTAC 7 cut(s) 120, 339, 376, 585, 811, 1076, 1146
CviAII CATG 9 cut(s) 72, 76, 114, 228, 256, 481, 614, 708, 716
CviQI GTAC 7 cut(s) 120, 339, 376, 585, 811, 1076, 1146
DdeI CTNAG 2 cut(s) 544, 962
DpnI GATC 2 cut(s) 146, 702
DpnII GATC 2 cut(s) 144, 700
EaeI YGGCCR 1 cut(s) 1005
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
EciI GGCGGA 2 cut(s) 43, 586
Eco147I AGGCCT 1 cut(s) 432
Eco47I GGWCC 2 cut(s) 476, 518
Eco88I CYCGRG 1 cut(s) 214
EcoO109I RGGNCCY 1 cut(s) 476
EcoRI GAATTC 1 cut(s) 407
EcoRII CCWGG 1 cut(s) 799
EcoT22I ATGCAT 3 cut(s) 77, 830, 1058
Esp3I CGTCTC 1 cut(s) 388
FaeI CATG 9 cut(s) 75, 79, 117, 231, 259, 484, 617, 711, 719
FalI AAGNNNNNCTT 5 cut(s) 37, 89, 121, 355, 387
FatI CATG 9 cut(s) 71, 75, 113, 227, 255, 480, 613, 707, 715
Fnu4HI GCNGC 6 cut(s) 57, 60, 111, 470, 1062, 1122
FokI GGATG 2 cut(s) 741, 1065
Fsp4HI GCNGC 6 cut(s) 57, 60, 111, 470, 1062, 1122
FspAI RTGCGCAY 1 cut(s) 612
FspBI CTAG 2 cut(s) 1070, 1085
FspI TGCGCA 1 cut(s) 612
GlaI GCGC 1 cut(s) 612
GluI GCNGC 6 cut(s) 57, 60, 111, 470, 1062, 1122
GsuI CTGGAG 1 cut(s) 783
HaeIII GGCC 6 cut(s) 233, 432, 698, 889, 1007, 1129
HapII CCGG 4 cut(s) 498, 558, 704, 1004
HhaI GCGC 1 cut(s) 613
Hin1I GRCGYC 1 cut(s) 381
Hin1II CATG 9 cut(s) 75, 79, 117, 231, 259, 484, 617, 711, 719
Hin6I GCGC 1 cut(s) 611
HinP1I GCGC 1 cut(s) 611
HincII GTYRAC 2 cut(s) 168, 181
HindII GTYRAC 2 cut(s) 168, 181
HindIII AAGCTT 1 cut(s) 364
HinfI GANTC 3 cut(s) 212, 805, 859
HpaII CCGG 4 cut(s) 498, 558, 704, 1004
HphI GGTGA 3 cut(s) 217, 312, 961
Hpy166II GTNNAC 5 cut(s) 42, 168, 181, 266, 457
Hpy188I TCNGA 1 cut(s) 438
Hpy188III TCNNGA 5 cut(s) 216, 487, 516, 682, 1141
Hpy8I GTNNAC 5 cut(s) 42, 168, 181, 266, 457
Hpy99I CGWCG 3 cut(s) 145, 383, 1142
HpyAV CCTTC 3 cut(s) 115, 443, 686
HpyCH4III ACNGT 6 cut(s) 39, 46, 178, 310, 338, 688
HpyCH4IV ACGT 3 cut(s) 381, 462, 593
HpyF10VI GCNNNNNNNGC 3 cut(s) 278, 704, 1031
HpyF3I CTNAG 2 cut(s) 544, 962
HpySE526I ACGT 3 cut(s) 381, 462, 593
Hsp92I GRCGYC 1 cut(s) 381
Hsp92II CATG 9 cut(s) 75, 79, 117, 231, 259, 484, 617, 711, 719
HspAI GCGC 1 cut(s) 611
KroI GCCGGC 1 cut(s) 1003
KroNI GCCGGC 1 cut(s) 1005
KspI CCGCGG 1 cut(s) 390
Kzo9I GATC 2 cut(s) 144, 700
LmnI GCTCC 1 cut(s) 1000
Lsp1109I GCAGC 3 cut(s) 481, 1048, 1108
LweI GCATC 6 cut(s) 719, 837, 843, 975, 1043, 1065
MaeI CTAG 2 cut(s) 1070, 1085
MaeII ACGT 3 cut(s) 381, 462, 593
MaeIII GTNAC 2 cut(s) 209, 332
MalI GATC 2 cut(s) 146, 702
MboI GATC 2 cut(s) 144, 700
MboII GAAGA 5 cut(s) 13, 79, 309, 904, 907
MhlI GDGCHC 1 cut(s) 459
MluCI AATT 6 cut(s) 154, 324, 407, 618, 777, 1019
MlyI GAGTC 1 cut(s) 206
MmeI TCCRAC 1 cut(s) 117
MnlI CCTC 6 cut(s) 61, 719, 746, 879, 1066, 1095
Mph1103I ATGCAT 3 cut(s) 77, 830, 1058
MroNI GCCGGC 1 cut(s) 1003
MroXI GAANNNNTTC 2 cut(s) 104, 586
MseI TTAA 3 cut(s) 327, 602, 1162
MslI CAYNNNNRTG 2 cut(s) 647, 665
MspA1I CMGCKG 1 cut(s) 389
MspI CCGG 4 cut(s) 498, 558, 704, 1004
MspR9I CCNGG 3 cut(s) 498, 559, 801
Mva1269I GAATGC 2 cut(s) 188, 507
MvaI CCWGG 1 cut(s) 801
MvnI CGCG 1 cut(s) 389
MwoI GCNNNNNNNGC 3 cut(s) 278, 704, 1031
NaeI GCCGGC 1 cut(s) 1005
NciI CCSGG 2 cut(s) 498, 559
NdeII GATC 2 cut(s) 144, 700
NgoMIV GCCGGC 1 cut(s) 1003
NlaIII CATG 9 cut(s) 75, 79, 117, 231, 259, 484, 617, 711, 719
NlaIV GGNNCC 5 cut(s) 478, 520, 541, 697, 1002
NmuCI GTSAC 1 cut(s) 209
NsbI TGCGCA 1 cut(s) 612
NsiI ATGCAT 3 cut(s) 77, 830, 1058
NspI RCATGY 1 cut(s) 117
PaeI GCATGC 1 cut(s) 117
PaeR7I CTCGAG 1 cut(s) 214
PceI AGGCCT 1 cut(s) 432
PctI GAATGC 2 cut(s) 188, 507
PdiI GCCGGC 1 cut(s) 1005
PdmI GAANNNNTTC 2 cut(s) 104, 586
PfeI GAWTC 2 cut(s) 805, 859
PflMI CCANNNNNTGG 3 cut(s) 636, 739, 1014
PfoI TCCNGGA 1 cut(s) 557
PkrI GCNGC 6 cut(s) 58, 61, 112, 471, 1063, 1123
PleI GAGTC 1 cut(s) 206
PpsI GAGTC 1 cut(s) 206
PpuMI RGGWCCY 1 cut(s) 476
Psp5II RGGWCCY 1 cut(s) 476
Psp6I CCWGG 1 cut(s) 799
PspGI CCWGG 1 cut(s) 799
PspN4I GGNNCC 5 cut(s) 478, 520, 541, 697, 1002
PspPI GGNCC 5 cut(s) 231, 476, 518, 696, 1127
PspPPI RGGWCCY 1 cut(s) 476
PstI CTGCAG 1 cut(s) 1126
RsaI GTAC 7 cut(s) 121, 340, 377, 586, 812, 1077, 1147
RsaNI GTAC 7 cut(s) 120, 339, 376, 585, 811, 1076, 1146
RseI CAYNNNNRTG 2 cut(s) 647, 665
SacII CCGCGG 1 cut(s) 390
SaqAI TTAA 3 cut(s) 327, 602, 1162
SatI GCNGC 6 cut(s) 57, 60, 111, 470, 1062, 1122
Sau3AI GATC 2 cut(s) 144, 700
Sau96I GGNCC 5 cut(s) 231, 476, 518, 696, 1127
ScaI AGTACT 3 cut(s) 121, 586, 1147
SchI GAGTC 1 cut(s) 206
ScrFI CCNGG 3 cut(s) 498, 559, 801
SduI GDGCHC 1 cut(s) 459
SfaNI GCATC 6 cut(s) 719, 837, 843, 975, 1043, 1065
SfcI CTRYAG 1 cut(s) 1122
Sfr274I CTCGAG 1 cut(s) 214
Sfr303I CCGCGG 1 cut(s) 390
SgrBI CCGCGG 1 cut(s) 390
SinI GGWCC 2 cut(s) 476, 518
SlaI CTCGAG 1 cut(s) 214
SmiMI CAYNNNNRTG 2 cut(s) 647, 665
SmlI CTYRAG 2 cut(s) 214, 680
SmoI CTYRAG 2 cut(s) 214, 680
SphI GCATGC 1 cut(s) 117
Sse9I AATT 6 cut(s) 154, 324, 407, 618, 777, 1019
SseBI AGGCCT 1 cut(s) 432
SsiI CCGC 9 cut(s) 54, 57, 60, 68, 111, 387, 389, 597, 869
SspI AATATT 2 cut(s) 917, 976
SspMI CTAG 2 cut(s) 1070, 1085
StuI AGGCCT 1 cut(s) 432
StyD4I CCNGG 3 cut(s) 496, 557, 799
TaaI ACNGT 6 cut(s) 39, 46, 178, 310, 338, 688
TaiI ACGT 3 cut(s) 384, 465, 596
TaqI TCGA 5 cut(s) 140, 215, 1100, 1117, 1140
TasI AATT 6 cut(s) 154, 324, 407, 618, 777, 1019
TatI WGTACW 4 cut(s) 119, 584, 810, 1145
TauI GCSGC 3 cut(s) 59, 62, 113
TfiI GAWTC 2 cut(s) 805, 859
Tru1I TTAA 3 cut(s) 327, 602, 1162
Tru9I TTAA 3 cut(s) 327, 602, 1162
TscAI CASTG 1 cut(s) 155
TseFI GTSAC 1 cut(s) 209
TseI GCWGC 3 cut(s) 469, 1061, 1121
Tsp45I GTSAC 1 cut(s) 209
TspDTI ATGAA 3 cut(s) 400, 704, 920
TspGWI ACGGA 1 cut(s) 153
TspRI CASTG 1 cut(s) 155
Van91I CCANNNNNTGG 3 cut(s) 636, 739, 1014
VneI GTGCAC 1 cut(s) 455
VpaK11BI GGWCC 2 cut(s) 476, 518
XapI RAATTY 3 cut(s) 407, 777, 1019
XceI RCATGY 1 cut(s) 117
XhoI CTCGAG 1 cut(s) 214
XmnI GAANNNNTTC 2 cut(s) 104, 586
XspI CTAG 2 cut(s) 1070, 1085
ZraI GACGTC 1 cut(s) 382
ZrmI AGTACT 3 cut(s) 121, 586, 1147
Zsp2I ATGCAT 3 cut(s) 77, 830, 1058
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.