Rorug02G0143100

DOMON domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002816
Physical Location & Seq
Reverse (-)
12571712 .. 12580139
8428 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0143100.1

Sequence Viewer

Length: 3150 bp
ATGGAAACCGATAACTCAGCGGACCTGAAGGTGGAGGAGCTCTTGAAAGAGGTCCAGCTAGAATATTCACCTGCGCTCACCAAGTTCGTGGACGACGCCGTTTCATCGATCAAAGCAGCCATCGATACAATTCCACAAGACCTCAAGGTTACGGCTGATTTAGCTCCGGGATTCGTCAGAGATATTGGTGCAGACAAAGTTGAATTTGATTTCAAGAAGCCGAAATCTGTCAAAATTGGCGGCAGTTATGCTATAGAGTGCCTTGTAAAGCCTGAGTTCAATGTCGATCTTTTCGTTCAGTTGCCCAAGGAATGTTTTCATGAGAAAGACTATTTCAATTACCGGTACCATGCTAAAAGGTGCCTGTACCTTTGTGTGATTAAAAAGTACTTGATTTCGTCTTCGTTAGTTGGTAAGGTTGAATGGTCCACTTTCCAGAACGAAGTCCGGAAACCTGTGTTGATTGTGTATCCAGTCACGAAATTAGTTGCACTCCCTGGGTTCTTTGTGAGAATAATTCCCACAGCACCATCTCTTTTTAGCATTCCAAAATTGAACCTGCAGCGGAATAATGTTCGTGCTGTGAGTAAAGGGGGCATTCCTCAGTCTACTCCCAAGTACAATAGTAGTATTTTGGAAGACATGTTTATAGAAGATACAGAAGAACTTGTCAAGCAAACTTTTCTTGGGTCAAAGGAATTACGACAAGGTTTAGTTTTGCTGAAGGTCTGGGCTCGCCGCAGGATGCCAATATATGCTTATGATTGCCTGAATGGTTTTCTAATCTCTGTCATATTGGCATACCTTGTGGATAGGAACCATATCAATAAATCAATGAAGGCAATGCAAATATTTCGTGTTACAATGAAATTCATTGCTACTTCAGATTTGTGGAAACACGGGCTCTACTTCACACCAAAAGGCCAAAAAGCTGTTTCAAAAGAGGAGAGGTTGCCTTTTAAAGAATCATTTCCAATTGTTATATGTACTCCATCTAGAAGCTTTAATATAGCCTTAAGGGTAACAAGGGTTGGATTTCTCGAGCTGCAAAATGAATCTACAATGACATTGGCATGCATTGAGAAATGCAGAGATAGTGGATTTGAGGAAGTATTTATGACCAGGATTGACTATCCTGTAAAATATGACCATGTCATAAGGTTGAACTTAAAGGGGAAGAGTTCAGTTTATGGATCGGGATTTTGCTTGGATGATGAATGTTGGAGAGTGTATGAGCAGAAGGTTCATAATGTTTTAAGTCATGGGTTGAGTGACCGGGTGAAGACTGTACGTGTGACCTGGAAAAGCATGTTATCAGAATCCATTCTACAAAATGGATTGTCGTCACTCAATACAGAGCCACTGCTAATTGGAATTTCAGTGAGCTCTTTAGATAAAGCTTTAAGAATTGTTGATATTGGTCCTGATGCTGATAATAAGGAGGAGGCTCTCAAATTCCGTCAGTTCTGGGGGGATAAGGCTGAGCTTAGGAGATTCAAAGATGGCAAGATAGCAGAAAGCACAGTTTGGGAAACCGAGCAATGGAAAAGACATATCGTCATAAAAAAGATATCTGAGCATGTGCTTCTGCGACATCTTTCACTATTGAAAGAGAATATCATGCATATCGTGGATCAACTTGATTTCTCTTTACTATATGGTGCCGAAGATCCTATATCTTCTTCCGGGAGTTTGTTTGGGGCATTTGAAATTCTATCGAAGCGATTGCGCCTTATTGAAGACATTCCTCTAAAGGTTTCTACAGTGCAGGCCCTAGATTCGGCTTTCAGGTATTCATCTGTCTTCCCACCTGAACCTCATCCGCTAGCTAATGAAAAGGGTTCTTTCGTAAGACTAAATCAGTTCCCTCCTTCCTGCATCCGGCCGCTGGAAGTTATGATCCAGTTGGAAGGTTCTGGAAACTGGCCTATGGATGATGTAGCAATTGAGAAAACTAAATCAGCATTCCTTCTTAAAATCGGAGAGAGTCTTCAGAATAGTTGGGGGATGACATGTACAGCCACTGAGGATGATGTGGATGTTTTTGTCTCTGGATATGCCTTTCGTCTTAAAATTTGGCATGAGAGAGGCCTGACTTTGTTGAGACGAGAAACTGGAAATGAGCACGTGAAGAAGGTTTCTAATGTGGACAAGGAACTATACTTTCGCAGTCAACATTCCAGCATGATAAATGGATTCCAGACTTGTTACCCAGCATATGGGCCAGTTGTTAGGCTTGCAAAACGATGGGCTGCTTCACATCTATTTTCGGCTTGCTTGGTAGAGGAGGCAATTGAGCTATTGGTAGCATATATATTTTTGAAGCCTTTACCATTTAATGCTCCATGCTCACGGATCACTGGATTTTTAAGGTTCCTGCGATTACTATCTGATTATGACTGGACCTTTTCTGCATTAGTTGTTGACATAAATAATGATTTAACATCAAATGATGAGAAAGAGATAAGGGAGAACTTCATGTTAAGTAGAAAAGGTTATGAAGAAAATCCTCAAAATGTAAACTCAGCAATGTTCCTAGCTACATCCTACGACAAGGCATCTGAGGCTTGGACCAAATTTTCACCAAATTCAATGGAGCTAAAAAGGTTGATGGCTTATGCTGGAAGCAGTGCAAACTTGTTGATCAAACTCATATTGGAGGACCATAGTGATTCTTATAGATGGGAGTGCCTTTTTCGAACTCCTTTGAACAATTATGATGCTGTAATTCTTCTCCATAGAGAAAAATTACCTTATCCCCAACGCCTTCTCTTTCCATCAGAATTACATCAAGGGGTTCATGTGGCACGTGGGAATGCAAGCAAGGGTTTCCACCCCTTTCTTTTGCCTGGAGATTTTAAAGGAAGCTCAGAGGATCTAAGAAACAAAGTATTGGTAAACTTCGATCCGCTAAGGTGTTTTGTTGGGGATCTAGAGAAAGAGTACTCAAACACTTTCAAGCTATGGTATGATTCTCTGGGTGGTGATGCTGTTGGCATAACATGGGGAGGATGTATTTCAAAGAAACGGGGCCGGGAAGAAGCAGATGAGGAAGTAAAGGATCCAATTGATTTGTTAAAAGATGTTGGTAAAGTTGGAACAGGGTTCGTCAGGGGCATTTATCTTCTAAAAGCCCCAAGGAGGTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Cytochrom_B561 DUF568

Protein Analysis

1049

Amino Acids

119.54

Weight (kDa)

8.45

Isoelectric Point (pI)

42.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nrap PF03813 95 - 227 4.9e-34 Nrap protein domain 1
Nrap_D2 PF17403 234 - 375 1.4e-30 Nrap protein PAP/OAS-like domain
Nrap_D3 PF17404 380 - 534 6.3e-38 Nrap protein domain 3
Nrap_D4 PF17405 556 - 735 3e-45 Nrap protein nucleotidyltransferase domain 4
Nrap_D5 PF17406 737 - 889 1.5e-46 Nrap protein PAP/OAS1-like domain 5
Nrap_D6 PF17407 903 - 1010 2.5e-12 Nrap protein domain 6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 79
Acc36I ACCTGC 2 cut(s) 79, 567
Acc65I GGTACC 1 cut(s) 345
AccB1I GGYRCC 3 cut(s) 345, 360, 1661
AccB7I CCANNNNNTGG 1 cut(s) 2219
AccI GTMKAC 1 cut(s) 608
AccIII TCCGGA 1 cut(s) 447
AciI CCGC 7 cut(s) 20, 240, 565, 739, 1823, 1886, 2909
AcoI YGGCCR 1 cut(s) 1883
AcsI RAATTY 8 cut(s) 203, 869, 1374, 1454, 1710, 2073, 2576, 2587
AcuI CTGAAG 4 cut(s) 47, 743, 867, 1976
AcvI CACGTG 2 cut(s) 2128, 2810
AcyI GRCGYC 1 cut(s) 96
AfaI GTAC 8 cut(s) 347, 368, 389, 620, 988, 1290, 2017, 2945
AfiI CCNNNNNNNGG 7 cut(s) 31, 1542, 1780, 1881, 1888, 2219, 3141
AflII CTTAAG 1 cut(s) 1015
AflIII ACRYGT 3 cut(s) 642, 1291, 2012
AgeI ACCGGT 1 cut(s) 342
AhdI GACNNNNNGTC 1 cut(s) 1556
AjnI CCWGG 4 cut(s) 496, 1121, 1298, 2848
Alw21I GWGCWC 3 cut(s) 42, 1388, 2127
Alw26I GTCTC 2 cut(s) 2053, 2098
AlwNI CAGNNNCTG 1 cut(s) 2024
Ama87I CYCGRG 1 cut(s) 1040
Aor13HI TCCGGA 1 cut(s) 447
AoxI GGCC 7 cut(s) 922, 1770, 1883, 1925, 2089, 2222, 3031
ApeKI GCWGC 4 cut(s) 116, 562, 1045, 2252
ApoI RAATTY 8 cut(s) 203, 869, 1374, 1454, 1710, 2073, 2576, 2587
AsiGI ACCGGT 1 cut(s) 342
Asp700I GAANNNNTTC 4 cut(s) 315, 969, 1323, 1743
Asp718I GGTACC 1 cut(s) 345
AspLEI GCGC 2 cut(s) 76, 1731
AsuC2I CCSGG 4 cut(s) 168, 1277, 1687, 3035
AsuHPI GGTGA 5 cut(s) 60, 70, 1291, 2574, 2996
AsuII TTCGAA 1 cut(s) 2698
AsuNHI GCTAGC 1 cut(s) 1825
AvaI CYCGRG 1 cut(s) 1040
AvaII GGWCC 7 cut(s) 22, 52, 426, 1421, 2403, 2571, 2662
BamHI GGATCC 1 cut(s) 3061
BanI GGYRCC 3 cut(s) 345, 360, 1661
BanII GRGCYC 4 cut(s) 42, 736, 906, 1388
BbrPI CACGTG 2 cut(s) 2128, 2810
BbsI GAAGAC 6 cut(s) 393, 645, 1289, 1746, 1795, 1982
Bbv12I GWGCWC 3 cut(s) 42, 1388, 2127
BbvI GCAGC 4 cut(s) 128, 574, 1032, 2239
BccI CCATC 8 cut(s) 128, 538, 1000, 1496, 2241, 2605, 2676, 2785
BceAI ACGGC 2 cut(s) 83, 168
BcgI CGANNNNNNTGC 4 cut(s) 836, 870, 1708, 1742
BciT130I CCWGG 4 cut(s) 498, 1123, 1300, 2850
BciVI GTATCC 1 cut(s) 480
BclI TGATCA 1 cut(s) 2643
BcnI CCSGG 4 cut(s) 168, 1277, 1687, 3035
BcoDI GTCTC 2 cut(s) 2053, 2098
BfaI CTAG 6 cut(s) 59, 996, 1775, 1826, 2537, 2933
BfmI CTRYAG 3 cut(s) 252, 560, 1761
BfrI CTTAAG 1 cut(s) 1015
BfuAI ACCTGC 2 cut(s) 79, 567
BfuI GTATCC 1 cut(s) 480
BisI GCNGC 7 cut(s) 117, 241, 563, 739, 1046, 1886, 2253
BlpI GCTNAGC 1 cut(s) 1482
BlsI GCNGC 7 cut(s) 118, 242, 564, 740, 1047, 1887, 2254
BmcAI AGTACT 2 cut(s) 389, 2945
Bme1390I CCNGG 8 cut(s) 168, 498, 1123, 1277, 1300, 1687, 2850, 3035
Bme18I GGWCC 7 cut(s) 22, 52, 426, 1421, 2403, 2571, 2662
BmeRI GACNNNNNGTC 1 cut(s) 1556
BmeT110I CYCGRG 1 cut(s) 1040
BmiI GGNNCC 7 cut(s) 347, 362, 818, 1663, 2375, 3032, 3063
BmrFI CCNGG 8 cut(s) 168, 498, 1123, 1277, 1300, 1687, 2850, 3035
BmsI GCATC 6 cut(s) 735, 1417, 1887, 2567, 2710, 2977
BmtI GCTAGC 1 cut(s) 1829
BpiI GAAGAC 6 cut(s) 393, 645, 1289, 1746, 1795, 1982
BpmI CTGGAG 1 cut(s) 2871
Bpu10I CCTNAGC 2 cut(s) 1487, 2912
Bpu1102I GCTNAGC 1 cut(s) 1482
Bpu14I TTCGAA 1 cut(s) 2698
BpuEI CTTGAG 1 cut(s) 128
BpuMI CCSGG 4 cut(s) 168, 1277, 1687, 3035
Bsa29I ATCGAT 2 cut(s) 107, 123
BsaAI YACGTR 3 cut(s) 1292, 2128, 2810
BsaBI GATNNNNATC 1 cut(s) 2386
BsaHI GRCGYC 1 cut(s) 96
BsaJI CCNNGG 4 cut(s) 306, 496, 497, 3137
BsaWI WCCGGW 2 cut(s) 342, 447
Bsc4I CCNNNNNNNGG 7 cut(s) 31, 1542, 1780, 1881, 1888, 2219, 3141
Bse118I RCCGGY 1 cut(s) 342
Bse1I ACTGG 7 cut(s) 473, 1903, 1928, 2119, 2225, 2365, 2405
Bse3DI GCAATG 4 cut(s) 849, 873, 1547, 2535
Bse8I GATNNNNATC 1 cut(s) 2386
BseAI TCCGGA 1 cut(s) 447
BseBI CCWGG 4 cut(s) 498, 1123, 1300, 2850
BseCI ATCGAT 2 cut(s) 107, 123
BseDI CCNNGG 4 cut(s) 306, 496, 497, 3137
BseJI GATNNNNATC 1 cut(s) 2386
BseLI CCNNNNNNNGG 7 cut(s) 31, 1542, 1780, 1881, 1888, 2219, 3141
BseMI GCAATG 4 cut(s) 849, 873, 1547, 2535
BseMII CTCAG 9 cut(s) 30, 264, 617, 1473, 1566, 2016, 2538, 2553, 2883
BseNI ACTGG 7 cut(s) 473, 1903, 1928, 2119, 2225, 2365, 2405
BseRI GAGGAG 4 cut(s) 50, 959, 1457, 2300
BseX3I CGGCCG 1 cut(s) 1883
BseXI GCAGC 4 cut(s) 128, 574, 1032, 2239
BseYI CCCAGC 1 cut(s) 2212
BsgI GTGCAG 2 cut(s) 210, 1787
Bsh1285I CGRYCG 1 cut(s) 1886
BshFI GGCC 7 cut(s) 924, 1772, 1885, 1927, 2091, 2224, 3033
BshNI GGYRCC 3 cut(s) 345, 360, 1661
BshTI ACCGGT 1 cut(s) 342
BshVI ATCGAT 2 cut(s) 107, 123
BsiEI CGRYCG 1 cut(s) 1886
BsiHKAI GWGCWC 3 cut(s) 42, 1388, 2127
BsiHKCI CYCGRG 1 cut(s) 1040
BsiSI CCGG 7 cut(s) 167, 343, 448, 1276, 1686, 1882, 3034
BslI CCNNNNNNNGG 7 cut(s) 31, 1542, 1780, 1881, 1888, 2219, 3141
BsmAI GTCTC 2 cut(s) 2053, 2098
BsmBI CGTCTC 1 cut(s) 2098
BsmI GAATGC 4 cut(s) 543, 597, 1964, 2821
BsnI GGCC 7 cut(s) 924, 1772, 1885, 1927, 2091, 2224, 3033
BsoBI CYCGRG 1 cut(s) 1040
Bsp119I TTCGAA 1 cut(s) 2698
Bsp1286I GDGCHC 5 cut(s) 42, 736, 906, 1388, 2127
Bsp13I TCCGGA 1 cut(s) 447
Bsp1407I TGTACA 1 cut(s) 2015
Bsp1720I GCTNAGC 1 cut(s) 1482
BspACI CCGC 7 cut(s) 20, 240, 565, 739, 1823, 1886, 2909
BspANI GGCC 7 cut(s) 924, 1772, 1885, 1927, 2091, 2224, 3033
BspCNI CTCAG 9 cut(s) 29, 265, 616, 1474, 1567, 2017, 2537, 2554, 2882
BspDI ATCGAT 2 cut(s) 107, 123
BspEI TCCGGA 1 cut(s) 447
BspHI TCATGA 1 cut(s) 319
BspLI GGNNCC 7 cut(s) 347, 362, 818, 1663, 2375, 3032, 3063
BspMAI CTGCAG 1 cut(s) 564
BspMI ACCTGC 2 cut(s) 79, 567
BspOI GCTAGC 1 cut(s) 1829
BspT104I TTCGAA 1 cut(s) 2698
BspT107I GGYRCC 3 cut(s) 345, 360, 1661
BspTI CTTAAG 1 cut(s) 1015
BsrDI GCAATG 4 cut(s) 849, 873, 1547, 2535
BsrFI RCCGGY 1 cut(s) 342
BsrGI TGTACA 1 cut(s) 2015
BsrI ACTGG 7 cut(s) 473, 1903, 1928, 2119, 2225, 2365, 2405
BssAI RCCGGY 1 cut(s) 342
BssECI CCNNGG 4 cut(s) 306, 496, 497, 3137
BssNI GRCGYC 1 cut(s) 96
BssT1I CCWWGG 2 cut(s) 306, 3137
Bst2UI CCWGG 4 cut(s) 498, 1123, 1300, 2850
Bst4CI ACNGT 3 cut(s) 1288, 1525, 1765
Bst6I CTCTTC 1 cut(s) 1172
BstACI GRCGYC 1 cut(s) 96
BstAFI CTTAAG 1 cut(s) 1015
BstAUI TGTACA 1 cut(s) 2015
BstBAI YACGTR 3 cut(s) 1292, 2128, 2810
BstBI TTCGAA 1 cut(s) 2698
BstC8I GCNNGC 7 cut(s) 736, 1075, 1770, 1827, 2238, 2275, 2821
BstHHI GCGC 2 cut(s) 76, 1731
BstMAI GTCTC 2 cut(s) 2053, 2098
BstMCI CGRYCG 1 cut(s) 1886
BstMWI GCNNNNNNNGC 2 cut(s) 161, 2564
BstNI CCWGG 4 cut(s) 498, 1123, 1300, 2850
BstNSI RCATGY 5 cut(s) 646, 1077, 1312, 1583, 2016
BstSCI CCNGG 8 cut(s) 166, 496, 1121, 1275, 1298, 1685, 2848, 3033
BstSFI CTRYAG 3 cut(s) 252, 560, 1761
BstV1I GCAGC 4 cut(s) 128, 574, 1032, 2239
BstV2I GAAGAC 6 cut(s) 393, 645, 1289, 1746, 1795, 1982
BstX2I RGATCY 4 cut(s) 1669, 2875, 2929, 3061
BstXI CCANNNNNNTGG 1 cut(s) 88
BstYI RGATCY 4 cut(s) 1669, 2875, 2929, 3061
BstZI CGGCCG 1 cut(s) 1883
Bsu15I ATCGAT 2 cut(s) 107, 123
BsuI GTATCC 1 cut(s) 480
BsuRI GGCC 7 cut(s) 924, 1772, 1885, 1927, 2091, 2224, 3033
BsuTUI ATCGAT 2 cut(s) 107, 123
BtsI GCAGTG 2 cut(s) 1361, 2635
BtsIMutI CAGTG 6 cut(s) 1361, 1386, 1770, 2022, 2358, 2635
BveI ACCTGC 2 cut(s) 79, 567
Cac8I GCNNGC 7 cut(s) 736, 1075, 1770, 1827, 2238, 2275, 2821
CaiI CAGNNNCTG 1 cut(s) 2024
CciI TCATGA 1 cut(s) 319
CfoI GCGC 2 cut(s) 76, 1731
Cfr10I RCCGGY 1 cut(s) 342
ClaI ATCGAT 2 cut(s) 107, 123
CseI GACGC 1 cut(s) 104
Csp6I GTAC 8 cut(s) 346, 367, 388, 619, 987, 1289, 2016, 2944
CspAI ACCGGT 1 cut(s) 342
CviQI GTAC 8 cut(s) 346, 367, 388, 619, 987, 1289, 2016, 2944
DraI TTTAAA 2 cut(s) 961, 2860
DriI GACNNNNNGTC 1 cut(s) 1556
EaeI YGGCCR 1 cut(s) 1883
EagI CGGCCG 1 cut(s) 1883
Eam1104I CTCTTC 1 cut(s) 1172
Eam1105I GACNNNNNGTC 1 cut(s) 1556
EarI CTCTTC 1 cut(s) 1172
Ecl136II GAGCTC 2 cut(s) 40, 1386
EclXI CGGCCG 1 cut(s) 1883
Eco130I CCWWGG 2 cut(s) 306, 3137
Eco147I AGGCCT 1 cut(s) 2091
Eco24I GRGCYC 4 cut(s) 42, 736, 906, 1388
Eco32I GATATC 1 cut(s) 1572
Eco47I GGWCC 7 cut(s) 22, 52, 426, 1421, 2403, 2571, 2662
Eco52I CGGCCG 1 cut(s) 1883
Eco53kI GAGCTC 2 cut(s) 40, 1386
Eco57I CTGAAG 4 cut(s) 47, 743, 867, 1976
Eco72I CACGTG 2 cut(s) 2128, 2810
Eco88I CYCGRG 1 cut(s) 1040
EcoICRI GAGCTC 2 cut(s) 40, 1386
EcoO109I RGGNCCY 1 cut(s) 1771
EcoRII CCWGG 4 cut(s) 496, 1121, 1298, 2848
EcoRV GATATC 1 cut(s) 1572
EcoT14I CCWWGG 2 cut(s) 306, 3137
EcoT22I ATGCAT 2 cut(s) 1079, 1626
EcoT38I GRGCYC 4 cut(s) 42, 736, 906, 1388
ErhI CCWWGG 2 cut(s) 306, 3137
Esp3I CGTCTC 1 cut(s) 2098
FauNDI CATATG 1 cut(s) 2218
FbaI TGATCA 1 cut(s) 2643
FblI GTMKAC 1 cut(s) 608
Fnu4HI GCNGC 7 cut(s) 117, 241, 563, 739, 1046, 1886, 2253
FriOI GRGCYC 4 cut(s) 42, 736, 906, 1388
Fsp4HI GCNGC 7 cut(s) 117, 241, 563, 739, 1046, 1886, 2253
FspBI CTAG 6 cut(s) 59, 996, 1775, 1826, 2537, 2933
GlaI GCGC 2 cut(s) 75, 1730
GluI GCNGC 7 cut(s) 117, 241, 563, 739, 1046, 1886, 2253
GsaI CCCAGC 1 cut(s) 2216
GsuI CTGGAG 1 cut(s) 2871
HaeIII GGCC 7 cut(s) 924, 1772, 1885, 1927, 2091, 2224, 3033
HapII CCGG 7 cut(s) 167, 343, 448, 1276, 1686, 1882, 3034
HgaI GACGC 1 cut(s) 104
HhaI GCGC 2 cut(s) 76, 1731
Hin1I GRCGYC 1 cut(s) 96
Hin6I GCGC 2 cut(s) 74, 1729
HinP1I GCGC 2 cut(s) 74, 1729
HincII GTYRAC 2 cut(s) 2174, 2425
HindII GTYRAC 2 cut(s) 2174, 2425
HindIII AAGCTT 2 cut(s) 1000, 1398
HpaII CCGG 7 cut(s) 167, 343, 448, 1276, 1686, 1882, 3034
HphI GGTGA 5 cut(s) 60, 70, 1291, 2574, 2996
Hpy166II GTNNAC 8 cut(s) 91, 429, 609, 2149, 2174, 2425, 2521, 2899
Hpy8I GTNNAC 8 cut(s) 91, 429, 609, 2149, 2174, 2425, 2521, 2899
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 9 cut(s) 22, 718, 832, 1234, 1881, 1904, 1979, 2128, 2777
HpyCH4III ACNGT 3 cut(s) 1288, 1525, 1765
HpyCH4IV ACGT 3 cut(s) 1291, 2127, 2809
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 2564
HpySE526I ACGT 3 cut(s) 1291, 2127, 2809
Hsp92I GRCGYC 1 cut(s) 96
HspAI GCGC 2 cut(s) 74, 1729
Kpn2I TCCGGA 1 cut(s) 447
KpnI GGTACC 1 cut(s) 349
Ksp22I TGATCA 1 cut(s) 2643
LmnI GCTCC 4 cut(s) 37, 169, 2347, 2596
Lsp1109I GCAGC 4 cut(s) 128, 574, 1032, 2239
LweI GCATC 6 cut(s) 735, 1417, 1887, 2567, 2710, 2977
MaeI CTAG 6 cut(s) 59, 996, 1775, 1826, 2537, 2933
MaeII ACGT 3 cut(s) 1291, 2127, 2809
MaeIII GTNAC 8 cut(s) 148, 475, 859, 1021, 1271, 1294, 1344, 2207
MfeI CAATTG 4 cut(s) 975, 1944, 2292, 3066
MflI RGATCY 4 cut(s) 1669, 2875, 2929, 3061
MhlI GDGCHC 5 cut(s) 42, 736, 906, 1388, 2127
MlyI GAGTC 1 cut(s) 1996
MmeI TCCRAC 4 cut(s) 1012, 1202, 1887, 3076
Mph1103I ATGCAT 2 cut(s) 1079, 1626
MroI TCCGGA 1 cut(s) 447
MroXI GAANNNNTTC 4 cut(s) 315, 969, 1323, 1743
MslI CAYNNNNRTG 1 cut(s) 1072
MspA1I CMGCKG 3 cut(s) 20, 565, 1888
MspCI CTTAAG 1 cut(s) 1015
MspI CCGG 7 cut(s) 167, 343, 448, 1276, 1686, 1882, 3034
MspR9I CCNGG 8 cut(s) 168, 498, 1123, 1277, 1300, 1687, 2850, 3035
MunI CAATTG 4 cut(s) 975, 1944, 2292, 3066
Mva1269I GAATGC 4 cut(s) 543, 597, 1964, 2821
MvaI CCWGG 4 cut(s) 498, 1123, 1300, 2850
MwoI GCNNNNNNNGC 2 cut(s) 161, 2564
NciI CCSGG 4 cut(s) 168, 1277, 1687, 3035
NdeI CATATG 1 cut(s) 2218
NheI GCTAGC 1 cut(s) 1825
NlaIV GGNNCC 7 cut(s) 347, 362, 818, 1663, 2375, 3032, 3063
NmuCI GTSAC 4 cut(s) 475, 1271, 1294, 1344
NsiI ATGCAT 2 cut(s) 1079, 1626
NspI RCATGY 5 cut(s) 646, 1077, 1312, 1583, 2016
NspV TTCGAA 1 cut(s) 2698
PaeI GCATGC 1 cut(s) 1077
PaeR7I CTCGAG 1 cut(s) 1040
PagI TCATGA 1 cut(s) 319
PaqCI CACCTGC 1 cut(s) 79
PasI CCCWGGG 1 cut(s) 497
PceI AGGCCT 1 cut(s) 2091
PciI ACATGT 2 cut(s) 642, 2012
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PctI GAATGC 4 cut(s) 543, 597, 1964, 2821
PdmI GAANNNNTTC 4 cut(s) 315, 969, 1323, 1743
PfeI GAWTC 9 cut(s) 171, 965, 1055, 1319, 1494, 1778, 2196, 2672, 2972
PflFI GACNNNGTC 1 cut(s) 1151
PflMI CCANNNNNTGG 1 cut(s) 2219
PfoI TCCNGGA 2 cut(s) 166, 1685
PinAI ACCGGT 1 cut(s) 342
PkrI GCNGC 7 cut(s) 118, 242, 564, 740, 1047, 1887, 2254
PleI GAGTC 1 cut(s) 1995
PmaCI CACGTG 2 cut(s) 2128, 2810
PmlI CACGTG 2 cut(s) 2128, 2810
PpsI GAGTC 1 cut(s) 1995
Ppu21I YACGTR 3 cut(s) 1292, 2128, 2810
PscI ACATGT 2 cut(s) 642, 2012
Psp124BI GAGCTC 2 cut(s) 42, 1388
Psp6I CCWGG 4 cut(s) 496, 1121, 1298, 2848
PspCI CACGTG 2 cut(s) 2128, 2810
PspFI CCCAGC 1 cut(s) 2212
PspGI CCWGG 4 cut(s) 496, 1121, 1298, 2848
PspN4I GGNNCC 7 cut(s) 347, 362, 818, 1663, 2375, 3032, 3063
PstI CTGCAG 1 cut(s) 564
PstNI CAGNNNCTG 1 cut(s) 2024
PsuI RGATCY 4 cut(s) 1669, 2875, 2929, 3061
PsyI GACNNNGTC 1 cut(s) 1151
RsaI GTAC 8 cut(s) 347, 368, 389, 620, 988, 1290, 2017, 2945
RsaNI GTAC 8 cut(s) 346, 367, 388, 619, 987, 1289, 2016, 2944
RseI CAYNNNNRTG 1 cut(s) 1072
SacI GAGCTC 2 cut(s) 42, 1388
SatI GCNGC 7 cut(s) 117, 241, 563, 739, 1046, 1886, 2253
ScaI AGTACT 2 cut(s) 389, 2945
SchI GAGTC 1 cut(s) 1996
ScrFI CCNGG 8 cut(s) 168, 498, 1123, 1277, 1300, 1687, 2850, 3035
SduI GDGCHC 5 cut(s) 42, 736, 906, 1388, 2127
SfaNI GCATC 6 cut(s) 735, 1417, 1887, 2567, 2710, 2977
SfcI CTRYAG 3 cut(s) 252, 560, 1761
Sfr274I CTCGAG 1 cut(s) 1040
SfuI TTCGAA 1 cut(s) 2698
SinI GGWCC 7 cut(s) 22, 52, 426, 1421, 2403, 2571, 2662
SlaI CTCGAG 1 cut(s) 1040
SmiMI CAYNNNNRTG 1 cut(s) 1072
SmlI CTYRAG 3 cut(s) 143, 1015, 1040
SmoI CTYRAG 3 cut(s) 143, 1015, 1040
SphI GCATGC 1 cut(s) 1077
SseBI AGGCCT 1 cut(s) 2091
SsiI CCGC 7 cut(s) 20, 240, 565, 739, 1823, 1886, 2909
SspI AATATT 2 cut(s) 65, 852
SspMI CTAG 6 cut(s) 59, 996, 1775, 1826, 2537, 2933
SstI GAGCTC 2 cut(s) 42, 1388
StuI AGGCCT 1 cut(s) 2091
StyD4I CCNGG 8 cut(s) 166, 496, 1121, 1275, 1298, 1685, 2848, 3033
StyI CCWWGG 2 cut(s) 306, 3137
TaaI ACNGT 3 cut(s) 1288, 1525, 1765
TaiI ACGT 3 cut(s) 1294, 2130, 2812
TaqI TCGA 7 cut(s) 107, 123, 285, 1041, 1718, 2698, 2904
TatI WGTACW 5 cut(s) 387, 618, 986, 2015, 2943
TauI GCSGC 3 cut(s) 243, 741, 1888
TfiI GAWTC 9 cut(s) 171, 965, 1055, 1319, 1494, 1778, 2196, 2672, 2972
TscAI CASTG 6 cut(s) 1368, 1386, 1770, 2029, 2365, 2635
TseFI GTSAC 4 cut(s) 475, 1271, 1294, 1344
TseI GCWGC 4 cut(s) 116, 562, 1045, 2252
Tsp45I GTSAC 4 cut(s) 475, 1271, 1294, 1344
TspGWI ACGGA 2 cut(s) 1448, 2368
TspRI CASTG 6 cut(s) 1368, 1386, 1770, 2029, 2365, 2635
Tth111I GACNNNGTC 1 cut(s) 1151
Van91I CCANNNNNTGG 1 cut(s) 2219
Vha464I CTTAAG 1 cut(s) 1015
VpaK11BI GGWCC 7 cut(s) 22, 52, 426, 1421, 2403, 2571, 2662
XapI RAATTY 8 cut(s) 203, 869, 1374, 1454, 1710, 2073, 2576, 2587
XbaI TCTAGA 2 cut(s) 995, 2932
XceI RCATGY 5 cut(s) 646, 1077, 1312, 1583, 2016
XhoI CTCGAG 1 cut(s) 1040
XmiI GTMKAC 1 cut(s) 608
XmnI GAANNNNTTC 4 cut(s) 315, 969, 1323, 1743
XspI CTAG 6 cut(s) 59, 996, 1775, 1826, 2537, 2933
ZrmI AGTACT 2 cut(s) 389, 2945
Zsp2I ATGCAT 2 cut(s) 1079, 1626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.