MD02G1186700.v1.1

Protein of unknown function (DUF568)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002816
Physical Location & Seq
Forward (+)
17000537 .. 17001703
1167 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1186700.v1.1.491

Sequence Viewer

Length: 1167 bp
ATGGCACTTTCCAGGTTTATCTTTGTTATTTGCTTACTGTTTACCGTCTTCTCTTCAGCCGCCGCACAACCGCCATGCATGAGCCGTACCTTCTCCAACAAGAAAACCTTCGCCGCATGCAGTACTCTGCCCGTTTTGAACTCGACGATCCACTGGAATTACTACCCGTCGACAGCCACGGTTGACATTGCATTCACACAATCTGTGGTGAGTGACTCGAGATGGGTTGCATGGGCCATAAACCCTACGTCCACTGGCATGGTCGGTTCACAAGCCATTGTCGCTTACAAAAGAACCGACGGAACTATTACTGTTTACTCTTCACCAATTAAAAGTTACGGGACACATTTGGAACAAGGGAATGTAAGCTTCCCTTTGTACGACGTCTCTGCGGTTTTTGAGAACAACGAATTCATCATCTTTGCAACCATAGGCCTTCCGAACAATGTCAGCGTTGTGCACCACGTCTGGCAGCAAGGACCCATGTTTGGTAACACCCCCGGAATGCACTCGCTTTCAGGACCCAACGTGCAATCCTTTGGAACCTTGGATTTTCTTTCCGGGAAAATTGAAACAAGCAAGGGAAGTACTTCAACGTCCGCCTTAAAATATGCGCATGGAATTATCAATACCATCAGTTGGGGCATACTTATGCCCATCGGCATAATCGTTGCACGGTATCTCAAGACAGTCGAAGGGGCTGATCCGGCATGGTTTCATGCTCACAGAGGATGCCAAATGCTGGCTTTTCTTGGAGGGATTGCAGGGTGGGGAACTGGAATTTTTCTTGGCTCTAAATCTCCGGGGATTCAGTACAAAGGGCATAGATGCATCGGCATCACTCTGTTTGCCCTTGCCACGATTCAAGTAGCGGTAGCTCTTTGTTTGAGGCCGAAGAAGACAGACGAGAATAGGAAATATTGGAACTGGTTTCATTGGGTTGTGGGGTATGGAACAATCGTCCTAAGCATCGTCAATATTTTCAAAGGATTTGACATATTGGAGCCGGCCAACAAGTGGAAATTTGCTTACATTGCCATCATCGGTACTTTGGGATGCATCGCTGCAATCCTAGAGCTACGAAAACTAGGCTCTTTCCTTTCGAGGTTTAGAAGCAATCGAGCTGCAGGGCAAGAAATAGTCGAGAGTACTCAAACAATAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561 DUF568

Protein Analysis

389

Amino Acids

42.4

Weight (kDa)

9.41

Isoelectric Point (pI)

32.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF568 PF04526 87 - 185 8.4e-25 Protein of unknown function (DUF568)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 387
Acc16I TGCGCA 1 cut(s) 615
AccB7I CCANNNNNTGG 3 cut(s) 639, 742, 1017
AccI GTMKAC 1 cut(s) 170
AciI CCGC 7 cut(s) 60, 63, 71, 114, 392, 600, 872
AclWI GGATC 2 cut(s) 142, 698
AcoI YGGCCR 1 cut(s) 1008
AcsI RAATTY 3 cut(s) 410, 780, 1022
AcuI CTGAAG 1 cut(s) 39
AcyI GRCGYC 1 cut(s) 384
AfaI GTAC 7 cut(s) 88, 124, 380, 589, 815, 1048, 1150
AfiI CCNNNNNNNGG 4 cut(s) 488, 639, 742, 1017
AgsI TTSAA 5 cut(s) 139, 572, 594, 866, 985
AjiI CACGTC 1 cut(s) 466
AjnI CCWGG 1 cut(s) 11
AluBI AGCT 4 cut(s) 369, 878, 1078, 1124
AluI AGCT 4 cut(s) 369, 878, 1078, 1124
Alw21I GWGCWC 1 cut(s) 462
Alw26I GTCTC 1 cut(s) 391
Alw44I GTGCAC 1 cut(s) 458
AlwI GGATC 2 cut(s) 142, 698
Ama87I CYCGRG 1 cut(s) 217
AoxI GGCC 4 cut(s) 234, 433, 890, 1008
ApaLI GTGCAC 1 cut(s) 458
ApeKI GCWGC 3 cut(s) 472, 1064, 1124
ApoI RAATTY 3 cut(s) 410, 780, 1022
Asp700I GAANNNNTTC 2 cut(s) 107, 589
AspLEI GCGC 1 cut(s) 616
AspS9I GGNCC 3 cut(s) 234, 479, 521
AsuC2I CCSGG 3 cut(s) 501, 562, 804
AsuHPI GGTGA 2 cut(s) 220, 315
AvaI CYCGRG 1 cut(s) 217
AvaII GGWCC 2 cut(s) 479, 521
BaeGI GKGCMC 1 cut(s) 462
BbsI GAAGAC 2 cut(s) 40, 905
Bbv12I GWGCWC 1 cut(s) 462
BbvI GCAGC 3 cut(s) 484, 1051, 1111
BccI CCATC 4 cut(s) 216, 641, 665, 1046
BceAI ACGGC 1 cut(s) 69
BcgI CGANNNNNNTGC 2 cut(s) 371, 405
BciT130I CCWGG 1 cut(s) 13
BcnI CCSGG 3 cut(s) 501, 562, 804
BcoDI GTCTC 1 cut(s) 391
BfaI CTAG 2 cut(s) 1073, 1088
BfmI CTRYAG 1 cut(s) 1125
BisI GCNGC 6 cut(s) 60, 63, 114, 473, 1065, 1125
BlsI GCNGC 6 cut(s) 61, 64, 115, 474, 1066, 1126
BmcAI AGTACT 3 cut(s) 124, 589, 1150
Bme1390I CCNGG 4 cut(s) 13, 501, 562, 804
Bme18I GGWCC 2 cut(s) 479, 521
BmeT110I CYCGRG 1 cut(s) 217
BmgBI CACGTC 1 cut(s) 466
BmgT120I GGNCC 3 cut(s) 234, 479, 521
BmiI GGNNCC 4 cut(s) 481, 523, 544, 1005
BmrFI CCNGG 4 cut(s) 13, 501, 562, 804
BmsI GCATC 7 cut(s) 722, 818, 840, 846, 978, 1046, 1068
BpiI GAAGAC 2 cut(s) 40, 905
Bpu10I CCTNAGC 1 cut(s) 965
BpuEI CTTGAG 1 cut(s) 668
BpuMI CCSGG 3 cut(s) 501, 562, 804
BsaHI GRCGYC 1 cut(s) 384
BsaJI CCNNGG 4 cut(s) 177, 499, 546, 803
Bsc4I CCNNNNNNNGG 4 cut(s) 488, 639, 742, 1017
Bse118I RCCGGY 1 cut(s) 1006
Bse1I ACTGG 4 cut(s) 158, 259, 781, 932
Bse3DI GCAATG 2 cut(s) 186, 1032
BseBI CCWGG 1 cut(s) 13
BseDI CCNNGG 4 cut(s) 177, 499, 546, 803
BseGI GGATG 2 cut(s) 737, 1061
BseLI CCNNNNNNNGG 4 cut(s) 488, 639, 742, 1017
BseMI GCAATG 2 cut(s) 186, 1032
BseNI ACTGG 4 cut(s) 158, 259, 781, 932
BseSI GKGCMC 1 cut(s) 462
BseXI GCAGC 3 cut(s) 484, 1051, 1111
BshFI GGCC 4 cut(s) 236, 435, 892, 1010
BsiHKAI GWGCWC 1 cut(s) 462
BsiHKCI CYCGRG 1 cut(s) 217
BsiSI CCGG 5 cut(s) 501, 561, 707, 803, 1007
BslFI GGGAC 1 cut(s) 355
BslI CCNNNNNNNGG 4 cut(s) 488, 639, 742, 1017
BsmAI GTCTC 1 cut(s) 391
BsmBI CGTCTC 1 cut(s) 391
BsmFI GGGAC 1 cut(s) 355
BsmI GAATGC 2 cut(s) 191, 510
BsnI GGCC 4 cut(s) 236, 435, 892, 1010
BsoBI CYCGRG 1 cut(s) 217
Bsp1286I GDGCHC 1 cut(s) 462
Bsp143I GATC 2 cut(s) 147, 703
BspACI CCGC 7 cut(s) 60, 63, 71, 114, 392, 600, 872
BspANI GGCC 4 cut(s) 236, 435, 892, 1010
BspLI GGNNCC 4 cut(s) 481, 523, 544, 1005
BspMAI CTGCAG 1 cut(s) 1129
BspPI GGATC 2 cut(s) 142, 698
BsrDI GCAATG 2 cut(s) 186, 1032
BsrFI RCCGGY 1 cut(s) 1006
BsrI ACTGG 4 cut(s) 158, 259, 781, 932
BssAI RCCGGY 1 cut(s) 1006
BssECI CCNNGG 4 cut(s) 177, 499, 546, 803
BssMI GATC 2 cut(s) 147, 703
BssNI GRCGYC 1 cut(s) 384
BssT1I CCWWGG 1 cut(s) 546
Bst2UI CCWGG 1 cut(s) 13
Bst4CI ACNGT 6 cut(s) 39, 46, 181, 313, 678, 691
Bst6I CTCTTC 2 cut(s) 58, 325
BstACI GRCGYC 1 cut(s) 384
BstC8I GCNNGC 3 cut(s) 118, 744, 1008
BstDEI CTNAG 1 cut(s) 965
BstDSI CCRYGG 1 cut(s) 177
BstF5I GGATG 2 cut(s) 737, 1061
BstHHI GCGC 1 cut(s) 616
BstKTI GATC 2 cut(s) 150, 706
BstMAI GTCTC 1 cut(s) 391
BstMBI GATC 2 cut(s) 147, 703
BstMWI GCNNNNNNNGC 3 cut(s) 281, 707, 1034
BstNI CCWGG 1 cut(s) 13
BstNSI RCATGY 1 cut(s) 120
BstSCI CCNGG 4 cut(s) 11, 499, 560, 802
BstSFI CTRYAG 1 cut(s) 1125
BstSLI GKGCMC 1 cut(s) 462
BstV1I GCAGC 3 cut(s) 484, 1051, 1111
BstV2I GAAGAC 2 cut(s) 40, 905
BstXI CCANNNNNNTGG 1 cut(s) 259
BsuRI GGCC 4 cut(s) 236, 435, 892, 1010
BtgI CCRYGG 1 cut(s) 177
BtgZI GCGATG 1 cut(s) 1045
BtrI CACGTC 1 cut(s) 466
BtsCI GGATG 2 cut(s) 737, 1061
BtsIMutI CAGTG 2 cut(s) 151, 252
Cac8I GCNNGC 3 cut(s) 118, 744, 1008
CfoI GCGC 1 cut(s) 616
Cfr10I RCCGGY 1 cut(s) 1006
Cfr13I GGNCC 3 cut(s) 234, 479, 521
Csp6I GTAC 7 cut(s) 87, 123, 379, 588, 814, 1047, 1149
CviAII CATG 9 cut(s) 75, 79, 117, 231, 259, 484, 617, 711, 719
CviQI GTAC 7 cut(s) 87, 123, 379, 588, 814, 1047, 1149
DdeI CTNAG 1 cut(s) 965
DpnI GATC 2 cut(s) 149, 705
DpnII GATC 2 cut(s) 147, 703
EaeI YGGCCR 1 cut(s) 1008
Eam1104I CTCTTC 2 cut(s) 58, 325
EarI CTCTTC 2 cut(s) 58, 325
EciI GGCGGA 1 cut(s) 589
Eco130I CCWWGG 1 cut(s) 546
Eco147I AGGCCT 1 cut(s) 435
Eco47I GGWCC 2 cut(s) 479, 521
Eco57I CTGAAG 1 cut(s) 39
Eco88I CYCGRG 1 cut(s) 217
EcoO109I RGGNCCY 2 cut(s) 479, 521
EcoRI GAATTC 1 cut(s) 410
EcoRII CCWGG 1 cut(s) 11
EcoT14I CCWWGG 1 cut(s) 546
EcoT22I ATGCAT 3 cut(s) 80, 833, 1061
ErhI CCWWGG 1 cut(s) 546
Esp3I CGTCTC 1 cut(s) 391
FaeI CATG 9 cut(s) 78, 82, 120, 234, 262, 487, 620, 714, 722
FalI AAGNNNNNCTT 4 cut(s) 92, 124, 358, 390
FaqI GGGAC 1 cut(s) 355
FatI CATG 9 cut(s) 74, 78, 116, 230, 258, 483, 616, 710, 718
FblI GTMKAC 1 cut(s) 170
Fnu4HI GCNGC 6 cut(s) 60, 63, 114, 473, 1065, 1125
FokI GGATG 2 cut(s) 744, 1068
Fsp4HI GCNGC 6 cut(s) 60, 63, 114, 473, 1065, 1125
FspAI RTGCGCAY 1 cut(s) 615
FspBI CTAG 2 cut(s) 1073, 1088
FspI TGCGCA 1 cut(s) 615
GlaI GCGC 1 cut(s) 615
GluI GCNGC 6 cut(s) 60, 63, 114, 473, 1065, 1125
HaeIII GGCC 4 cut(s) 236, 435, 892, 1010
HapII CCGG 5 cut(s) 501, 561, 707, 803, 1007
HhaI GCGC 1 cut(s) 616
Hin1I GRCGYC 1 cut(s) 384
Hin1II CATG 9 cut(s) 78, 82, 120, 234, 262, 487, 620, 714, 722
Hin6I GCGC 1 cut(s) 614
HinP1I GCGC 1 cut(s) 614
HincII GTYRAC 2 cut(s) 171, 184
HindII GTYRAC 2 cut(s) 171, 184
HindIII AAGCTT 1 cut(s) 367
HinfI GANTC 3 cut(s) 215, 808, 862
HpaII CCGG 5 cut(s) 501, 561, 707, 803, 1007
HphI GGTGA 2 cut(s) 220, 315
Hpy166II GTNNAC 7 cut(s) 42, 171, 184, 252, 269, 316, 460
Hpy188I TCNGA 1 cut(s) 441
Hpy188III TCNNGA 4 cut(s) 219, 519, 685, 1144
Hpy8I GTNNAC 7 cut(s) 42, 171, 184, 252, 269, 316, 460
Hpy99I CGWCG 4 cut(s) 148, 172, 302, 386
HpyAV CCTTC 4 cut(s) 100, 118, 446, 689
HpyCH4III ACNGT 6 cut(s) 39, 46, 181, 313, 678, 691
HpyCH4IV ACGT 5 cut(s) 248, 384, 465, 528, 596
HpyF10VI GCNNNNNNNGC 3 cut(s) 281, 707, 1034
HpyF3I CTNAG 1 cut(s) 965
HpySE526I ACGT 5 cut(s) 248, 384, 465, 528, 596
Hsp92I GRCGYC 1 cut(s) 384
Hsp92II CATG 9 cut(s) 78, 82, 120, 234, 262, 487, 620, 714, 722
HspAI GCGC 1 cut(s) 614
KroI GCCGGC 1 cut(s) 1006
KroNI GCCGGC 1 cut(s) 1008
Kzo9I GATC 2 cut(s) 147, 703
LmnI GCTCC 1 cut(s) 1003
Lsp1109I GCAGC 3 cut(s) 484, 1051, 1111
LweI GCATC 7 cut(s) 722, 818, 840, 846, 978, 1046, 1068
MaeI CTAG 2 cut(s) 1073, 1088
MaeII ACGT 5 cut(s) 248, 384, 465, 528, 596
MaeIII GTNAC 3 cut(s) 212, 335, 491
MalI GATC 2 cut(s) 149, 705
MboI GATC 2 cut(s) 147, 703
MboII GAAGA 5 cut(s) 40, 45, 312, 907, 910
MhlI GDGCHC 1 cut(s) 462
MluCI AATT 7 cut(s) 157, 327, 410, 567, 621, 780, 1022
MlyI GAGTC 1 cut(s) 209
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 4 cut(s) 722, 749, 882, 1098
Mph1103I ATGCAT 3 cut(s) 80, 833, 1061
MroNI GCCGGC 1 cut(s) 1006
MroXI GAANNNNTTC 2 cut(s) 107, 589
MseI TTAA 2 cut(s) 330, 605
MslI CAYNNNNRTG 2 cut(s) 257, 650
MspI CCGG 5 cut(s) 501, 561, 707, 803, 1007
MspR9I CCNGG 4 cut(s) 13, 501, 562, 804
Mva1269I GAATGC 2 cut(s) 191, 510
MvaI CCWGG 1 cut(s) 13
MwoI GCNNNNNNNGC 3 cut(s) 281, 707, 1034
NaeI GCCGGC 1 cut(s) 1008
NciI CCSGG 3 cut(s) 501, 562, 804
NdeII GATC 2 cut(s) 147, 703
NgoMIV GCCGGC 1 cut(s) 1006
NlaIII CATG 9 cut(s) 78, 82, 120, 234, 262, 487, 620, 714, 722
NlaIV GGNNCC 4 cut(s) 481, 523, 544, 1005
NmuCI GTSAC 1 cut(s) 212
NsbI TGCGCA 1 cut(s) 615
NsiI ATGCAT 3 cut(s) 80, 833, 1061
NspI RCATGY 1 cut(s) 120
PaeI GCATGC 1 cut(s) 120
PaeR7I CTCGAG 1 cut(s) 217
PceI AGGCCT 1 cut(s) 435
PcsI WCGNNNNNNNCGW 1 cut(s) 666
PctI GAATGC 2 cut(s) 191, 510
PdiI GCCGGC 1 cut(s) 1008
PdmI GAANNNNTTC 2 cut(s) 107, 589
PfeI GAWTC 2 cut(s) 808, 862
PflMI CCANNNNNTGG 3 cut(s) 639, 742, 1017
PfoI TCCNGGA 1 cut(s) 560
PkrI GCNGC 6 cut(s) 61, 64, 115, 474, 1066, 1126
PleI GAGTC 1 cut(s) 209
PpsI GAGTC 1 cut(s) 209
PpuMI RGGWCCY 2 cut(s) 479, 521
Psp5II RGGWCCY 2 cut(s) 479, 521
Psp6I CCWGG 1 cut(s) 11
PspGI CCWGG 1 cut(s) 11
PspN4I GGNNCC 4 cut(s) 481, 523, 544, 1005
PspPI GGNCC 3 cut(s) 234, 479, 521
PspPPI RGGWCCY 2 cut(s) 479, 521
PstI CTGCAG 1 cut(s) 1129
RsaI GTAC 7 cut(s) 88, 124, 380, 589, 815, 1048, 1150
RsaNI GTAC 7 cut(s) 87, 123, 379, 588, 814, 1047, 1149
RseI CAYNNNNRTG 2 cut(s) 257, 650
SalI GTCGAC 1 cut(s) 169
SaqAI TTAA 2 cut(s) 330, 605
SatI GCNGC 6 cut(s) 60, 63, 114, 473, 1065, 1125
Sau3AI GATC 2 cut(s) 147, 703
Sau96I GGNCC 3 cut(s) 234, 479, 521
ScaI AGTACT 3 cut(s) 124, 589, 1150
SchI GAGTC 1 cut(s) 209
ScrFI CCNGG 4 cut(s) 13, 501, 562, 804
SduI GDGCHC 1 cut(s) 462
SfaNI GCATC 7 cut(s) 722, 818, 840, 846, 978, 1046, 1068
SfcI CTRYAG 1 cut(s) 1125
Sfr274I CTCGAG 1 cut(s) 217
SinI GGWCC 2 cut(s) 479, 521
SlaI CTCGAG 1 cut(s) 217
SmiMI CAYNNNNRTG 2 cut(s) 257, 650
SmlI CTYRAG 2 cut(s) 217, 683
SmoI CTYRAG 2 cut(s) 217, 683
SphI GCATGC 1 cut(s) 120
Sse9I AATT 7 cut(s) 157, 327, 410, 567, 621, 780, 1022
SseBI AGGCCT 1 cut(s) 435
SsiI CCGC 7 cut(s) 60, 63, 71, 114, 392, 600, 872
SspI AATATT 2 cut(s) 920, 979
SspMI CTAG 2 cut(s) 1073, 1088
StuI AGGCCT 1 cut(s) 435
StyD4I CCNGG 4 cut(s) 11, 499, 560, 802
StyI CCWWGG 1 cut(s) 546
TaaI ACNGT 6 cut(s) 39, 46, 181, 313, 678, 691
TaiI ACGT 5 cut(s) 251, 387, 468, 531, 599
TaqI TCGA 7 cut(s) 143, 170, 218, 693, 1103, 1120, 1143
TasI AATT 7 cut(s) 157, 327, 410, 567, 621, 780, 1022
TatI WGTACW 4 cut(s) 122, 587, 813, 1148
TauI GCSGC 3 cut(s) 62, 65, 116
TfiI GAWTC 2 cut(s) 808, 862
Tru1I TTAA 2 cut(s) 330, 605
Tru9I TTAA 2 cut(s) 330, 605
TscAI CASTG 2 cut(s) 158, 259
TseFI GTSAC 1 cut(s) 212
TseI GCWGC 3 cut(s) 472, 1064, 1124
Tsp45I GTSAC 1 cut(s) 212
TspDTI ATGAA 3 cut(s) 403, 707, 923
TspGWI ACGGA 1 cut(s) 315
TspRI CASTG 2 cut(s) 158, 259
Van91I CCANNNNNTGG 3 cut(s) 639, 742, 1017
VneI GTGCAC 1 cut(s) 458
VpaK11BI GGWCC 2 cut(s) 479, 521
XapI RAATTY 3 cut(s) 410, 780, 1022
XceI RCATGY 1 cut(s) 120
XhoI CTCGAG 1 cut(s) 217
XmiI GTMKAC 1 cut(s) 170
XmnI GAANNNNTTC 2 cut(s) 107, 589
XspI CTAG 2 cut(s) 1073, 1088
ZraI GACGTC 1 cut(s) 385
ZrmI AGTACT 3 cut(s) 124, 589, 1150
Zsp2I ATGCAT 3 cut(s) 80, 833, 1061
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.