pycom02g15060

Protein of unknown function (DUF568)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002816
Physical Location & Seq
Reverse (-)
11771761 .. 11772855
1095 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g15060.1

Sequence Viewer

Length: 1095 bp
ATGCACGACCCATGTCTTCTCCAACAAGAAAACCTTCGCCGCATGCAGTACTCTGCCCGTTTTGAACTCGACGATCGACCATGGAATTACTATCCGTCGACAGCCACGGTTGACATTGCATTCACACAATCTGTGGTGAGTGACTCGAGATGGGTTGCATGGGCCATAAACCCTACGTCTACTGGCATGGTCGGTTCACAAGCCATTGTCGCTTACAAAAGAACCGATGGAACTATGACGGTTTATTCTTCACCGATTAAAAGTTACGGTACTCATTTGGAACAAGGGAATGTAAGCTTCCCTTTGTACGACGTCTCCGCGGTTTATGAGAACAACGAATTCATCATCTTTGCGACCATAGGCCTTCCGAACAATGTCAGCGTTGTGCACCACGTCTGGCAGCAAGGACCCATGTTCGGGAACACCCCCGGAATGCACTCGCTTTCCGGACCCAATGTGCAATCTTTTGGAACCTTAGATTTTCTTTCCGGGAAAACTGAAACAAGCAAGGGAACTACTTCAACGTGCCCCTTAAAATATGCGCATGGTATTATCAATACCATCAGTTGGGGCATACTTATGCCCATCGGCATAATCGTGGCAAGGTATCTCAAGACGGTTGAAGGGGCCGATCCGGCATGGTTTCACGCTCATAGAGGATGCCAAATGCTGGCTTTTCTTGGAGGGATTGCAGGGTGGGGAACTGGAATTTTTCTTGGCTCTAAATCTCCGGGGATTCAGTACAAAGGGCATAGATGCATCGGCATCACTCTGTTTGTCCTTGCCACGATTCAAGTAGCGGTAGCTCTTTGTTTGAGGCCAAAGAAGACAGACGAGAATAGGAAATATTGGAACTGGTTTCATTGGGTTGTGGGGTATGGAACAATCGTCCTAAGCATCACCAATATTTTCAAAGGATTTGACATATTGGAGCCGGCAAACAAGTGGAAATTTGCTTACATTGCCATCATCGGTACTTTGGGATGCATCGCTGCAATCCTAGAGCTACGAAAACTGGGCTCTTTCCTTTCGAGGTTTAGAAGCAGTCGAGCTGCAGGGCCAGAAATCGTCGAGAGTACTCAAACAATAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561 DUF568

Protein Analysis

365

Amino Acids

40.25

Weight (kDa)

9.04

Isoelectric Point (pI)

30.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF568 PF04526 63 - 161 1.1e-25 Protein of unknown function (DUF568)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 315
Acc16I TGCGCA 1 cut(s) 543
AccB7I CCANNNNNTGG 2 cut(s) 567, 670
AccI GTMKAC 2 cut(s) 98, 179
AccII CGCG 1 cut(s) 320
AccIII TCCGGA 1 cut(s) 446
AciI CCGC 4 cut(s) 40, 318, 320, 800
AclWI GGATC 1 cut(s) 626
AcsI RAATTY 3 cut(s) 338, 708, 950
AcyI GRCGYC 1 cut(s) 312
AfaI GTAC 6 cut(s) 50, 271, 308, 743, 976, 1078
AfiI CCNNNNNNNGG 4 cut(s) 416, 417, 567, 670
AgsI TTSAA 5 cut(s) 65, 522, 623, 794, 913
AjiI CACGTC 1 cut(s) 394
AluBI AGCT 4 cut(s) 297, 806, 1006, 1052
AluI AGCT 4 cut(s) 297, 806, 1006, 1052
Alw21I GWGCWC 1 cut(s) 390
Alw26I GTCTC 1 cut(s) 319
Alw44I GTGCAC 1 cut(s) 386
AlwI GGATC 1 cut(s) 626
Ama87I CYCGRG 1 cut(s) 145
Aor13HI TCCGGA 1 cut(s) 446
AoxI GGCC 5 cut(s) 162, 361, 627, 818, 1058
ApaLI GTGCAC 1 cut(s) 386
ApeKI GCWGC 3 cut(s) 400, 992, 1052
ApoI RAATTY 3 cut(s) 338, 708, 950
Asp700I GAANNNNTTC 2 cut(s) 33, 517
AspLEI GCGC 1 cut(s) 544
AspS9I GGNCC 5 cut(s) 162, 407, 449, 627, 1058
AsuC2I CCSGG 3 cut(s) 429, 490, 732
AsuHPI GGTGA 3 cut(s) 148, 243, 892
AvaI CYCGRG 1 cut(s) 145
AvaII GGWCC 2 cut(s) 407, 449
BaeGI GKGCMC 2 cut(s) 390, 530
BanII GRGCYC 1 cut(s) 1022
BbsI GAAGAC 2 cut(s) 8, 833
Bbv12I GWGCWC 1 cut(s) 390
BbvI GCAGC 3 cut(s) 412, 979, 1039
BccI CCATC 5 cut(s) 144, 221, 569, 593, 974
BcnI CCSGG 3 cut(s) 429, 490, 732
BcoDI GTCTC 1 cut(s) 319
BfaI CTAG 1 cut(s) 1001
BfmI CTRYAG 1 cut(s) 1053
BglI GCCNNNNNGGC 1 cut(s) 635
BisI GCNGC 4 cut(s) 40, 401, 993, 1053
BlsI GCNGC 4 cut(s) 41, 402, 994, 1054
BmcAI AGTACT 2 cut(s) 50, 1078
Bme1390I CCNGG 3 cut(s) 429, 490, 732
Bme18I GGWCC 2 cut(s) 407, 449
BmeT110I CYCGRG 1 cut(s) 145
BmgBI CACGTC 1 cut(s) 394
BmgT120I GGNCC 5 cut(s) 162, 407, 449, 627, 1058
BmiI GGNNCC 5 cut(s) 409, 451, 472, 628, 933
BmrFI CCNGG 3 cut(s) 429, 490, 732
BmrI ACTGGG 1 cut(s) 1025
BmsI GCATC 7 cut(s) 650, 746, 768, 774, 906, 974, 996
BmuI ACTGGG 1 cut(s) 1025
BoxI GACNNNNGTC 1 cut(s) 12
BpiI GAAGAC 2 cut(s) 8, 833
Bpu10I CCTNAGC 1 cut(s) 893
BpuEI CTTGAG 1 cut(s) 596
BpuMI CCSGG 3 cut(s) 429, 490, 732
BsaHI GRCGYC 1 cut(s) 312
BsaJI CCNNGG 5 cut(s) 80, 105, 318, 427, 731
BsaWI WCCGGW 1 cut(s) 446
BsaXI ACNNNNNCTCC 2 cut(s) 299, 329
Bsc4I CCNNNNNNNGG 4 cut(s) 416, 417, 567, 670
Bse118I RCCGGY 1 cut(s) 934
Bse1I ACTGG 4 cut(s) 187, 709, 860, 1020
Bse3DI GCAATG 2 cut(s) 114, 960
BseAI TCCGGA 1 cut(s) 446
BseDI CCNNGG 5 cut(s) 80, 105, 318, 427, 731
BseGI GGATG 2 cut(s) 665, 989
BseLI CCNNNNNNNGG 4 cut(s) 416, 417, 567, 670
BseMI GCAATG 2 cut(s) 114, 960
BseNI ACTGG 4 cut(s) 187, 709, 860, 1020
BseSI GKGCMC 2 cut(s) 390, 530
BseXI GCAGC 3 cut(s) 412, 979, 1039
Bsh1236I CGCG 1 cut(s) 320
Bsh1285I CGRYCG 1 cut(s) 76
BshFI GGCC 5 cut(s) 164, 363, 629, 820, 1060
BsiEI CGRYCG 1 cut(s) 76
BsiHKAI GWGCWC 1 cut(s) 390
BsiHKCI CYCGRG 1 cut(s) 145
BsiSI CCGG 6 cut(s) 429, 447, 489, 635, 731, 935
BslI CCNNNNNNNGG 4 cut(s) 416, 417, 567, 670
BsmAI GTCTC 1 cut(s) 319
BsmBI CGTCTC 1 cut(s) 319
BsmI GAATGC 2 cut(s) 119, 438
BsnI GGCC 5 cut(s) 164, 363, 629, 820, 1060
BsoBI CYCGRG 1 cut(s) 145
Bsp1286I GDGCHC 3 cut(s) 390, 530, 1022
Bsp13I TCCGGA 1 cut(s) 446
Bsp143I GATC 2 cut(s) 73, 631
Bsp19I CCATGG 1 cut(s) 80
BspACI CCGC 4 cut(s) 40, 318, 320, 800
BspANI GGCC 5 cut(s) 164, 363, 629, 820, 1060
BspEI TCCGGA 1 cut(s) 446
BspFNI CGCG 1 cut(s) 320
BspLI GGNNCC 5 cut(s) 409, 451, 472, 628, 933
BspMAI CTGCAG 1 cut(s) 1057
BspPI GGATC 1 cut(s) 626
BsrDI GCAATG 2 cut(s) 114, 960
BsrFI RCCGGY 1 cut(s) 934
BsrI ACTGG 4 cut(s) 187, 709, 860, 1020
BssAI RCCGGY 1 cut(s) 934
BssECI CCNNGG 5 cut(s) 80, 105, 318, 427, 731
BssMI GATC 2 cut(s) 73, 631
BssNI GRCGYC 1 cut(s) 312
BssT1I CCWWGG 1 cut(s) 80
Bst4CI ACNGT 4 cut(s) 109, 241, 269, 619
BstACI GRCGYC 1 cut(s) 312
BstC8I GCNNGC 3 cut(s) 44, 672, 936
BstDEI CTNAG 2 cut(s) 475, 893
BstDSI CCRYGG 3 cut(s) 80, 105, 318
BstF5I GGATG 2 cut(s) 665, 989
BstFNI CGCG 1 cut(s) 320
BstHHI GCGC 1 cut(s) 544
BstKTI GATC 2 cut(s) 76, 634
BstMAI GTCTC 1 cut(s) 319
BstMBI GATC 2 cut(s) 73, 631
BstMCI CGRYCG 1 cut(s) 76
BstMWI GCNNNNNNNGC 3 cut(s) 209, 635, 962
BstNSI RCATGY 1 cut(s) 46
BstPAI GACNNNNGTC 1 cut(s) 12
BstSCI CCNGG 3 cut(s) 427, 488, 730
BstSFI CTRYAG 1 cut(s) 1053
BstSLI GKGCMC 2 cut(s) 390, 530
BstUI CGCG 1 cut(s) 320
BstV1I GCAGC 3 cut(s) 412, 979, 1039
BstV2I GAAGAC 2 cut(s) 8, 833
BsuRI GGCC 5 cut(s) 164, 363, 629, 820, 1060
BtgI CCRYGG 3 cut(s) 80, 105, 318
BtgZI GCGATG 1 cut(s) 973
BtrI CACGTC 1 cut(s) 394
BtsCI GGATG 2 cut(s) 665, 989
Cac8I GCNNGC 3 cut(s) 44, 672, 936
CfoI GCGC 1 cut(s) 544
Cfr10I RCCGGY 1 cut(s) 934
Cfr13I GGNCC 5 cut(s) 162, 407, 449, 627, 1058
Cfr42I CCGCGG 1 cut(s) 321
Csp6I GTAC 6 cut(s) 49, 270, 307, 742, 975, 1077
CviAII CATG 8 cut(s) 12, 43, 81, 159, 187, 412, 545, 639
CviQI GTAC 6 cut(s) 49, 270, 307, 742, 975, 1077
DdeI CTNAG 2 cut(s) 475, 893
DpnI GATC 2 cut(s) 75, 633
DpnII GATC 2 cut(s) 73, 631
Eco130I CCWWGG 1 cut(s) 80
Eco147I AGGCCT 1 cut(s) 363
Eco24I GRGCYC 1 cut(s) 1022
Eco47I GGWCC 2 cut(s) 407, 449
Eco88I CYCGRG 1 cut(s) 145
EcoO109I RGGNCCY 1 cut(s) 407
EcoRI GAATTC 1 cut(s) 338
EcoT14I CCWWGG 1 cut(s) 80
EcoT22I ATGCAT 2 cut(s) 761, 989
EcoT38I GRGCYC 1 cut(s) 1022
ErhI CCWWGG 1 cut(s) 80
Esp3I CGTCTC 1 cut(s) 319
FaeI CATG 8 cut(s) 15, 46, 84, 162, 190, 415, 548, 642
FalI AAGNNNNNCTT 4 cut(s) 18, 50, 286, 318
FatI CATG 8 cut(s) 11, 42, 80, 158, 186, 411, 544, 638
FblI GTMKAC 2 cut(s) 98, 179
Fnu4HI GCNGC 4 cut(s) 40, 401, 993, 1053
FokI GGATG 2 cut(s) 672, 996
FriOI GRGCYC 1 cut(s) 1022
Fsp4HI GCNGC 4 cut(s) 40, 401, 993, 1053
FspAI RTGCGCAY 1 cut(s) 543
FspBI CTAG 1 cut(s) 1001
FspI TGCGCA 1 cut(s) 543
GlaI GCGC 1 cut(s) 543
GluI GCNGC 4 cut(s) 40, 401, 993, 1053
HaeIII GGCC 5 cut(s) 164, 363, 629, 820, 1060
HapII CCGG 6 cut(s) 429, 447, 489, 635, 731, 935
HhaI GCGC 1 cut(s) 544
Hin1I GRCGYC 1 cut(s) 312
Hin1II CATG 8 cut(s) 15, 46, 84, 162, 190, 415, 548, 642
Hin6I GCGC 1 cut(s) 542
HinP1I GCGC 1 cut(s) 542
HincII GTYRAC 2 cut(s) 99, 112
HindII GTYRAC 2 cut(s) 99, 112
HindIII AAGCTT 1 cut(s) 295
HinfI GANTC 3 cut(s) 143, 736, 790
HpaII CCGG 6 cut(s) 429, 447, 489, 635, 731, 935
HphI GGTGA 3 cut(s) 148, 243, 892
Hpy166II GTNNAC 5 cut(s) 99, 112, 180, 197, 388
Hpy188I TCNGA 1 cut(s) 369
Hpy188III TCNNGA 5 cut(s) 147, 418, 447, 613, 1072
Hpy8I GTNNAC 5 cut(s) 99, 112, 180, 197, 388
Hpy99I CGWCG 4 cut(s) 74, 100, 314, 1073
HpyAV CCTTC 3 cut(s) 44, 374, 617
HpyCH4III ACNGT 4 cut(s) 109, 241, 269, 619
HpyCH4IV ACGT 4 cut(s) 176, 312, 393, 524
HpyF10VI GCNNNNNNNGC 3 cut(s) 209, 635, 962
HpyF3I CTNAG 2 cut(s) 475, 893
HpySE526I ACGT 4 cut(s) 176, 312, 393, 524
Hsp92I GRCGYC 1 cut(s) 312
Hsp92II CATG 8 cut(s) 15, 46, 84, 162, 190, 415, 548, 642
HspAI GCGC 1 cut(s) 542
Kpn2I TCCGGA 1 cut(s) 446
KroI GCCGGC 1 cut(s) 934
KroNI GCCGGC 1 cut(s) 936
KspI CCGCGG 1 cut(s) 321
Kzo9I GATC 2 cut(s) 73, 631
LmnI GCTCC 1 cut(s) 931
Lsp1109I GCAGC 3 cut(s) 412, 979, 1039
LweI GCATC 7 cut(s) 650, 746, 768, 774, 906, 974, 996
MaeI CTAG 1 cut(s) 1001
MaeII ACGT 4 cut(s) 176, 312, 393, 524
MaeIII GTNAC 2 cut(s) 140, 263
MalI GATC 2 cut(s) 75, 633
MboI GATC 2 cut(s) 73, 631
MboII GAAGA 3 cut(s) 8, 240, 838
MhlI GDGCHC 3 cut(s) 390, 530, 1022
MluCI AATT 4 cut(s) 85, 338, 708, 950
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 1 cut(s) 46
MnlI CCTC 4 cut(s) 650, 677, 810, 1026
Mph1103I ATGCAT 2 cut(s) 761, 989
MroI TCCGGA 1 cut(s) 446
MroNI GCCGGC 1 cut(s) 934
MroXI GAANNNNTTC 2 cut(s) 33, 517
MseI TTAA 2 cut(s) 258, 533
MslI CAYNNNNRTG 2 cut(s) 578, 596
MspA1I CMGCKG 1 cut(s) 320
MspI CCGG 6 cut(s) 429, 447, 489, 635, 731, 935
MspR9I CCNGG 3 cut(s) 429, 490, 732
Mva1269I GAATGC 2 cut(s) 119, 438
MvnI CGCG 1 cut(s) 320
MwoI GCNNNNNNNGC 3 cut(s) 209, 635, 962
NaeI GCCGGC 1 cut(s) 936
NciI CCSGG 3 cut(s) 429, 490, 732
NcoI CCATGG 1 cut(s) 80
NdeII GATC 2 cut(s) 73, 631
NgoMIV GCCGGC 1 cut(s) 934
NlaIII CATG 8 cut(s) 15, 46, 84, 162, 190, 415, 548, 642
NlaIV GGNNCC 5 cut(s) 409, 451, 472, 628, 933
NmuCI GTSAC 1 cut(s) 140
NsbI TGCGCA 1 cut(s) 543
NsiI ATGCAT 2 cut(s) 761, 989
NspI RCATGY 1 cut(s) 46
PaeI GCATGC 1 cut(s) 46
PaeR7I CTCGAG 1 cut(s) 145
PceI AGGCCT 1 cut(s) 363
PcsI WCGNNNNNNNCGW 1 cut(s) 594
PctI GAATGC 2 cut(s) 119, 438
PdiI GCCGGC 1 cut(s) 936
PdmI GAANNNNTTC 2 cut(s) 33, 517
PfeI GAWTC 2 cut(s) 736, 790
PflMI CCANNNNNTGG 2 cut(s) 567, 670
PfoI TCCNGGA 1 cut(s) 488
PkrI GCNGC 4 cut(s) 41, 402, 994, 1054
Ple19I CGATCG 1 cut(s) 76
PleI GAGTC 1 cut(s) 137
PpsI GAGTC 1 cut(s) 137
PpuMI RGGWCCY 1 cut(s) 407
PshAI GACNNNNGTC 1 cut(s) 12
Psp5II RGGWCCY 1 cut(s) 407
PspN4I GGNNCC 5 cut(s) 409, 451, 472, 628, 933
PspPI GGNCC 5 cut(s) 162, 407, 449, 627, 1058
PspPPI RGGWCCY 1 cut(s) 407
PstI CTGCAG 1 cut(s) 1057
PvuI CGATCG 1 cut(s) 76
RsaI GTAC 6 cut(s) 50, 271, 308, 743, 976, 1078
RsaNI GTAC 6 cut(s) 49, 270, 307, 742, 975, 1077
RseI CAYNNNNRTG 2 cut(s) 578, 596
SacII CCGCGG 1 cut(s) 321
SalI GTCGAC 1 cut(s) 97
SaqAI TTAA 2 cut(s) 258, 533
SatI GCNGC 4 cut(s) 40, 401, 993, 1053
Sau3AI GATC 2 cut(s) 73, 631
Sau96I GGNCC 5 cut(s) 162, 407, 449, 627, 1058
ScaI AGTACT 2 cut(s) 50, 1078
SchI GAGTC 1 cut(s) 137
ScrFI CCNGG 3 cut(s) 429, 490, 732
SduI GDGCHC 3 cut(s) 390, 530, 1022
SfaNI GCATC 7 cut(s) 650, 746, 768, 774, 906, 974, 996
SfcI CTRYAG 1 cut(s) 1053
Sfr274I CTCGAG 1 cut(s) 145
Sfr303I CCGCGG 1 cut(s) 321
SgrBI CCGCGG 1 cut(s) 321
SinI GGWCC 2 cut(s) 407, 449
SlaI CTCGAG 1 cut(s) 145
SmiMI CAYNNNNRTG 2 cut(s) 578, 596
SmlI CTYRAG 2 cut(s) 145, 611
SmoI CTYRAG 2 cut(s) 145, 611
SphI GCATGC 1 cut(s) 46
Sse9I AATT 4 cut(s) 85, 338, 708, 950
SseBI AGGCCT 1 cut(s) 363
SsiI CCGC 4 cut(s) 40, 318, 320, 800
SspI AATATT 2 cut(s) 848, 907
SspMI CTAG 1 cut(s) 1001
StuI AGGCCT 1 cut(s) 363
StyD4I CCNGG 3 cut(s) 427, 488, 730
StyI CCWWGG 1 cut(s) 80
TaaI ACNGT 4 cut(s) 109, 241, 269, 619
TaiI ACGT 4 cut(s) 179, 315, 396, 527
TaqI TCGA 7 cut(s) 69, 76, 98, 146, 1031, 1048, 1071
TasI AATT 4 cut(s) 85, 338, 708, 950
TatI WGTACW 3 cut(s) 48, 741, 1076
TauI GCSGC 1 cut(s) 42
TfiI GAWTC 2 cut(s) 736, 790
Tru1I TTAA 2 cut(s) 258, 533
Tru9I TTAA 2 cut(s) 258, 533
TseFI GTSAC 1 cut(s) 140
TseI GCWGC 3 cut(s) 400, 992, 1052
Tsp45I GTSAC 1 cut(s) 140
TspDTI ATGAA 2 cut(s) 331, 851
TspGWI ACGGA 1 cut(s) 84
Van91I CCANNNNNTGG 2 cut(s) 567, 670
VneI GTGCAC 1 cut(s) 386
VpaK11BI GGWCC 2 cut(s) 407, 449
XapI RAATTY 3 cut(s) 338, 708, 950
XceI RCATGY 1 cut(s) 46
XhoI CTCGAG 1 cut(s) 145
XmiI GTMKAC 2 cut(s) 98, 179
XmnI GAANNNNTTC 2 cut(s) 33, 517
XspI CTAG 1 cut(s) 1001
ZraI GACGTC 1 cut(s) 313
ZrmI AGTACT 2 cut(s) 50, 1078
Zsp2I ATGCAT 2 cut(s) 761, 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.